CN104981477A - Device and method for performing blood thromboelastographic assays by magnetic sensing - Google Patents
Device and method for performing blood thromboelastographic assays by magnetic sensing Download PDFInfo
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- CN104981477A CN104981477A CN201380044224.6A CN201380044224A CN104981477A CN 104981477 A CN104981477 A CN 104981477A CN 201380044224 A CN201380044224 A CN 201380044224A CN 104981477 A CN104981477 A CN 104981477A
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Abstract
The invention provides a magnetic sensor elastometry device (MSED) and a method to perform the whole blood thromboelastography assay. It contains key components, including sample cuvette, detecting head, rotating disc, optical motion detector, and etc; and measures viscoelasticity of whole blood samples. The device optically monitors the physical motion of the magnetically driven rotating disc immersed in the blood sample. The thromboelastograph is recorded by the optical motion detector reading high pulse counts through a gated time window passing through the rotating disc. The device also includes a microcontroller and its embedded firmware to perform the functions of driving the rotating magnetic disc, generating high-frequency pulses, controlling the data pulse time window, as well as handling the user's interface, data analysis, and maintaining communication with an external computer.
Description
This application claims the right of priority that the sequence number submitted on August 22nd, 2012 is the U.S. Provisional Patent Application of 61/692,141.
Technical field
Present invention relates in general to blood coagulation elasticity and trace mensuration.More particularly, the present invention be a kind of for surveyed by magnetic strength perform whole blood blood coagulation elasticity trace measure so as to test that blood clot is formed, the equipment easily of retraction and/or cracking process and method.
Background technology
The accurate assessment of the efficiency of coagulation of blood (blood coagulation elasticity is traced) is hemorrhage for treatment, wound and be very important for various different anesthesia and surgical procedure.
Condensation is that wherein blood forms the process of clot.Coagulation of blood (i.e. thrombosis) is the result of a series of biochemical reactions by primary hemostatic and secondary hemostasis.Briefly, primary hemostatic relates to platelet adhesion reaction and gathering; Secondary hemostasis relates to blood plasma factor and to react each other and Fibrinogen changes into crosslinking polymerization scleroproein by some enzyme reactions.Coagulation of blood stops losing blood from the blood vessel damaged, the vessel wall wherein damaged covered by thrombocyte and fibre-bearing fibrin clot in case stop bleeding and repair the blood vessel damaged.The obstacle of this condensation may cause bleeding (hemorrhage) or the risk of obstructive blood coagulation (thrombosis) increases.
Condensation usually almost immediately in the damage to blood vessel in be lining in the endothelium of blood vessel after start.The vessel wall damaged is exposed to the startup that exposed wound tissue causes by the tissue factor being expressed as integral membrane proteins condensing.Tissue factor is tied to the enzyme reaction that blood plasma factor VIIa starts the short blood coagulation plasma proteins causing zymoplasm to be formed, causing platelet activation and urging coagulation complexes is subsequently assembled on platelet surface, causes change fibrinogen to become to strengthen the crosslinked fibrin grumeleuse of thrombocyte bolt.This condensation cascade reaction is done strictly to regulate and control in order to the various different endogenous antithrombotics of the balance between maintaining blood coagulation and bleeding by the different step in path.
Conventional condensation measures for be bled by wound and the hemorrhage operation caused and coagulopathy has poor precision of prediction.These measure usually in the external execution not having thrombocyte and other hemocytes; And hemostatic function can not be tested for hypothermia patient.Utilize the method for these routines, clearly can not distinguish the physiopathology of bleeding of associating with traumatic coagulopathy and a large amount of intraoperative blood loss, thus make the alternative of blood products be difficult.Wound is one of global major causes of death.Hemorrhagely account for 40% of the death that wound causes.The coagulopathy associated with severe injury makes the control from being complicated of bleeding, and is associated with the increase of the M & M of trauma patient.Quick diagnosis and have the intervention of guidance can reduce severe injury after preventible death.The origin cause of formation with the coagulopathy of the patient of severe trauma is multifactorial, comprises consumption and the dilution of thrombocyte and coagulation factor, and the dysfunction of thrombocyte and condensed system.Hypothermia, oxypathy and the dilution from standard resuscitation may worsen the coagulopathy that presents and continue to bleed.
Prevent from significant coagulopathy and effectively control the serious strategy of bleeding when there is coagulopathy reducing transfusion requirement, thus improve the clinical effectiveness with the patient of severe trauma.
In addition, the hemorrhage major cause remaining combat death in wound injures and deaths.Therefore, strong robust equipment of wishing a kind of overall hemostatic function assessed in the advance theater of war.Similarly, the peri-operation period of coagulation of blood diagnosis and monitor for understanding hemorrhage reason better, instruct hemostatic treatment and prediction continued narcosis or surgical procedure during the risk of bleeding be crucial.For experience major operation or be in such as wound, hemorrhage, apoplexy or septicemia and so on emergency situations under patient for, the coagulation of blood assessment of learning in time is determining that patient easily suffers from postoperative thrombotic complications or as early sepsis, especially about the use of blood products and guidance utilize the telltale of the treatment of hemostatic compositions in the urgent need to.
About the test of thromboelastography, usually little blood sample (typically 0.36ml) is placed in cuvette, this cuvette rotates 4 ° of 6 minutes cycling times of 45'(gently) to imitate Venous flow and activate condensation slowly.When sensor is inserted in sample, grumeleuse will be formed between cuvette and sensor.The speed that grumeleuse is formed and intensity can be measured in a variety of ways; And other factors depending on the activity of plasmatic coagulation system, platelet function, fibrinolytic and may affect by disease, environment and medication.If suspected due to medication or disease, blood is difficult to condense into block, so before immediately testing, blood sample is exposed to grumeleuse inductor (such as kaolin).
The first blood coagulation elasticity graphy method is introduced in 1948 by Hartert, and has made some amendments for many years to improve this technology.Hartert introduces the cylindrical member be arranged on rigidly in solid frame and the beaker being arranged on bar-like support upper end, and this bar-like support is installed in solid frame by means of round and elastic barrier film.The coil device of bar lower end produces rotary electromagnetic field, and gives bar track motion.Another core of diaphragm is used as the pick device of the trace of the changes in amplitude recording resiliency supported when grumeleuse is formed.This allows more in detail and accurately records grumeleuse forming process.Haemoscope company further improves this technology and the driving ring comprising moment of torsion sensing post and arrange around post main body.This device comprises the first guide shaft and glass stand that are fixed to driving ring rigidly further.This invention causes current Haemoscope TEG equipment.
Technical progress causes blood coagulation elasticity to trace some development of technology.
equipment (Haemoscope company, Illinois, the U.S.) and
equipment (PentapharmGmbh, Germany) the two measure grumeleuse visco-elasticity and provide the clinical grumeleuse that lower patient is set of peri-operation period to be formed and the speed of fibrinolytic process, strength and stability is especially to recover about the instruction of blood products.But, current
with
both technology all use frangible moving parts (rotating dog or rotating cup) and complicated mechanical component, and this makes equipment arrange lower in point-of care or be difficult to use in the theater of war under war condition.Current
with
the detection signal used in technology obtains by torsion wire or by the light change that kinetoscope reflects.Current
or
the shortcoming of technology also comprises low precision, muting sensitivity (signal to noise ratio) and comes the interference of self-excited oscillation, limited portability and process the difficulty of whole blood sample.
Also the other technologies of the blood Flexible change detected when grumeleuse is formed are developed.Wherein, Sonoclot analyser (Sienco company, A Wada, Colorado) uses the hollow open disposable plastic detector be arranged in transducer head, and wherein this detector vertically vibrates at test period.The change of the impedance for motion that the grumeleuse formed gradually applies is recorded.But Sonoclot tests trace and is considered to quite qualitatively in its clinical application.
And the maximum grumeleuse soundness of these technology is about 50-70mm.Consider that general level of sensitivity is about 2mm, higher test-test change is almost inevitable.This is especially a serious problem when measuring sample during the thrombocyte suppression for Fibrinogen exception is tested.
Up to now, do not exist a kind of clinician of permission bedside accurately manage massive transfusion and monitor haemostatic state in time so as effectively management association bleed and make that the best of correct diagnosis and blood products uses simple, reliably and diagnostic test fast.
Therefore, the object of this invention is to provide a kind of fast and be easy to trace mensuration for performing blood coagulation elasticity, especially measure blood clotting formation, the equipment of grumeleuse synthesis speed, clot strength and blood fibrinolytic degree and method.
Accompanying drawing explanation
Fig. 1 utilizes the exploded view detecting sub-component to depict a preferred embodiment of the present invention.
Fig. 2 depicts an embodiment of sample cuvette.
Fig. 3 depicts an embodiment of disposable detection head.
Fig. 4 depicts an embodiment of coupling/receiving cavity.
Fig. 5 depicts an embodiment of rotating disk.
Fig. 6 depicts an embodiment of the dynamo-electric rotating magnetic field generator based on step unit.
Fig. 7 depicts an embodiment of the dynamo-electric rotating magnetic field generator based on static coil.
Fig. 8 is isometric view of the present invention.
Fig. 9 is the block diagram of fundamental system and special electronic device.
Figure 10 is the oscillogram that demonstration detects the basic sequential in front end.
The typical trace of Figure 11 for tracing for the blood coagulation elasticity of " TEG level-1 ".
The typical trace of Figure 12 for tracing for the blood coagulation elasticity of " TEG level-2 ".
Figure 13 is the typical trace traced for the blood coagulation elasticity of normal people's whole blood.
Figure 14 is the typical trace traced for the blood coagulation elasticity of abnormal people's whole blood.
Embodiment
All explanations of drawing and description of the present invention, all for describing the object of selected version of the present invention, are not intended to limit scope of the present invention.
The present invention relates to a kind of equipment and the method for tracing mensuration for performing whole blood blood coagulation elasticity.It also relates to and is a kind ofly easy to use, accurately and fast for the handheld device at bedside, doctor's office, Operation theatre or test patient blood even afield.The present invention's (Magnetic Sensor elasticity measurement equipment (MSED)) measures the viscoelasticity property of whole blood.At test period, upon activation of condensation cascade reaction, no matter be by intrinsic pathway, extrinsic pathway or the combination of both, all form zymoplasm.Zymoplasm splits into fibrin monomer by former for soluble fibrin, and these monomers spontaneously aggregate into crosslinked fibrin network.The peculiar property of this network structure is, it shows as the rigid elastic solid of the distortion shear-stress can resisting fluid flow blood.
An object of the present invention is to provide a kind of for perform especially but be not limited to the beginning in clotting time, the blood coagulation elasticity of the degree of speed that grumeleuse is formed, clot strength, fibrinolytic process traces the equipment of mensuration.The medicine instructing the parameter of the use of blood products and/or intra-operative to use also is application of the present invention.
Another object of the present invention is the sample cup and the rotating disk that use coaxial orientation.The distortion shear-stress caused by the rotating disk immersed in test blood sample detects at himself (at rotating disk place) and measures.
Another object of the present invention is to provide the resistance of control and balance deformation stress and whole blood fibrin network to measure the device of true visco-elasticity change when blood clot is formed.
Another object of the present invention is to provide the device utilizing accurate sense technology to detect the resistance of blood clot with small mechanical disturbance, such as, use the optical pickup of the gating technology of the high impulse pattern being used for data counts.
The present invention includes be arranged in sample rack heated by heat block in case provide the sample cuvette of the constant temperature of 37 DEG C, rotating disk and the disposable detection head by the coupling of docking cavity, the magnetic field generator generator for rotating disk, the axle stop pin of dish axle and shaft collar, quartz-timer and with the optical motion detector of optical amplifier together with signal gate control circuit.
The disposable sensing head be made up of the plastics spring shape wing is inserted into by coupled chamber and detects in rotating disk.When handle component immerses in sample cuvette, add reagent to trigger hemostasis.The firmware of proprietary microcontroller and embedding thereof performs and drives spinning disk at a predetermined velocity back and forth, generate high-frequency impulse at fluorescence detector place, control time window for collecting pulse counting, read the optics gate data of autorotation disk to obtain the function of the trace that blood coagulation elasticity traces etc.Other expansion implementations of the present invention also allow the ability realizing simultaneously running multiple blood sample and/or mensuration.
With reference to Fig. 1-9, one embodiment of the present of invention comprise sample cuvette 101, disposable detection head 102, the coupling of sensing dish and receive cavity 103, sensing dish 104, magnetic field generator 902, and this magnetic field generator comprises the dynamo-electric rotating magnetic field generator 601 based on step unit and the dynamo-electric rotating magnetic field generator 701 based on static coil.Fig. 1 is the exploded view detecting sub-component, comprise: (1) cuvette 101 (holding the blood sample of test), (2) detection head 102, (3) coupling of sensing dish and receiving cavity 103, (4) rotating disk 104, (5) axle 105 is coiled, (6) axle stop pin 106, (7) optical motion detector 107, (8) optical amplifier and signal gate control circuit 108, (9) heat block 109 of the constant temperature of 37 DEG C is provided, (10) dynamo-electric rotating magnetic field generator 601 and 701 and (11) block well heater 110.
In the present invention, the visco-elasticity of hemostasis can be measured by being placed to by disposable detection head 102 to contact with the whole blood sample 901 being placed in sample cuvette 101.Blood heat maintains 37 DEG C by means of computer-controlled heating sampling cabin (bay).Detection head 102 is via coupling and receive the coupling mechanism temporary attachment of cavity 103 to magnetic rotating disk 104.So this dish is moved by the rotatingfield below cuvette accomodating unit.Originally, detection head/rotary disc assembly does not have appreciablely lingeringly to follow magnetic rotation campaign immediately.As time goes by, once coagulation reagent is added in sample, the visco-elasticity of blood sample changes during grumeleuse forming process, and detection head/rotary disc assembly motion response change, show the slow speed proportional with current blood visco elasticity.This process was continued by the regular hour, allowed the visco-elasticity change that blood reaches the highest, and only allowed detection head/rotating disk sub-component to follow magnetic rotation campaign.With this understanding, rotating disk 104 is by continuation campaign, but the visco-elasticity level that the blood sample with test reaches reduces by its movement velocity pro rata.
The combination of this equipment use cylindrical cup and coupling shear-induced dish, and employ allow the visco-elasticity when blood clot is formed change after carry out the optics gate assembly of precise signal collection.In one embodiment of the invention, system uses a kind of method of viscoelastic property of novel unique detection test sample book.This novel method make use of the physical change and electromechanical movement parameter of adding whole blood sample after reagent.When testing beginning, High speed pulse generator runs (Figure 10-A), and system microcontroller 906 provides energy to magnetic power driving mechanism 908, the increment rotary motion (Figure 10-B) of described driving mechanism generation dish 104 and detection head 102.Dish 104 has and comprises equidistant some angular movements (3 equal angles of such as each 15 degree are moved; Or the motion of each 7.5 degree 6 equal angles) miter angle degree rotating range.Meanwhile, microcontroller 906 allows the first step (Figure 10-C) realizing the pulse gate control logic allowing high-speed pulse to pass through.Optical motion detector 107 monitors rotating disk 104.Second gating circuit (Figure 10-D) of high-speed pulse is enabled or is forbidden in the output of amplifying; It will be read (Figure 10-E) by microcontroller 906.The time lag producing speed and the dish rotated with dish 104 is responded into " time window " of direct ratio by motion detector 107.Therefore, just when reagent being added to whole blood sample 901, this liquid mixture shows minimum visco-elasticity value; And the response of coiling 104 is almost instant, does not have appreciable time lag.Therefore, " gate duration window " is very short, and a small amount of high-speed pulse only allowing High speed pulse generator 904 to generate passes through.As time goes by, the visco-elasticity change of test sample book; And this forces dish 104 to make its speed of rotation slow down and starts to cause postpone time response.Therefore, among the multistage gate duration window for reading, the first gate duration window is enabled by microcontroller 906; And the second gate duration window is controlled by the angular velocity of rotation of rotating disk 104.As a result, " gate duration window " is elongated, and allows the pulse of larger quantity to pass through.When the present invention is simplified enforcement, some default conditions are set up, comprise the well width on the frequency of High speed pulse generator 904, rotating disk 104, the angle of light beam and width, distribute to microcontroller 906 to read the time of the pulse arrived, and be set to every incremental angle and move the best rotatingfield speed of 1875 milliseconds.
In addition, blood coagulation elasticity traces reagent for causing the controlled visco-elasticity change of the blood sample held in sample cuvette 101.Reagent is calcium chloride, a kind of reagent of freeze-drying or air-dry reagent.And blood coagulation elasticity traces reagent also can comprise kaolin, tissue factor, heparinase, platelet suppressant drug or Trypsin inhibitor,Trasylol.
In one embodiment of the invention, process electronic unit be shown in (Fig. 9) in system chart as follows: by external AC-DC power adapters feed-in and be equipped with electric pressure controlling device and distribute 911 power supply; Optical motion detector 107; Optical amplifier and signal gate 108; High speed pulse generator 904; Pulse gate control logic 905; The analog line driver 907 of well heater; Block well heater 110; Magnetic power driving mechanism 908 is gathered; The clock generator 912 of microcontroller; The system microcontroller 906 based on flash memory that 8 CMOS strengthen; Communication port 913; 12V DC input socket 909; Comprise ON/OFF power switch 910, lcd screen, the user interface 914 of hummer and keypad can be listened.Complete system also can comprise outside or inside computer.
When equipment is energized, system microcontroller 906 will perform following action in order: 1) in order to safety and minimizing power dissipation, forbid all analog line drivers immediately; 2) user interface initialize: LCD, hummer and keypad; 3) indicating meter initiation message; 4) in order to the built in self testing of integrity of firmware; 5) if find any mistake, then show self-detection result, and wait for the confirmation of operator; 6) welcome message and firmware version number is shown; 7) input of operator is waited for; 8) just suitable service temperature monitors cuvette cabin (heat block); 9) communication port is monitored; 10) when reaching presumptive test temperature, display " system is ready " prompting; 11) input command of operator is waited for; 12) operator is instructed to pass through test process via LCD message; 13) detection arm position is monitored; 14) run blood coagulation elasticity and trace test; 15) pick-up transducers raw data and transmit this data via communication port; 16) calculate, store and display result; And 17) wait for the input of operator.
In one embodiment of the invention, whole blood sample volume is 260 microlitres together with the reagent of 40 microlitres.Especially, follow these steps and perform the test of whole blood thrombus elasticity: 1) device power; 2) on LCD, welcome message and firmware version number is shown; 3) system cloud gray model automatic self; 4) just correct temperature checkout cabin (cuvette heat block); 5) once reach described temperature; Display " ready " message; 6) system instruction operator promotes detection arm; 7) disposable detection head is inserted in prompting; 8) cuvette is placed in test chamber; 9) whole blood sample is transported in cuvette by instruction; 10) wait for that blood sample reaches suitable probe temperature; 11) once sample reaches 37 DEG C, instruction operator delivery of therapeutic agents; 12) start test, collect data and send data to principal computer; 13) message of MSED equipment display instruction test end; 14) raw data of computer reception is stored for not visiting; 15) respective graphical curve is manifested on the computer screen; 16) post-processing algorithm (baseline subtraction, multiple spot smoothing filter and bipolar figure) is applied for evaluating further and final test analysis; And 17) total test duration can be set to 12,24,36 and 60 minutes.
With reference to Figure 11-14, give some illustrative examples.Detailed research discloses, when starting test " TEG level-1 " sample, umber of pulse typically is low (each about 70 of reading) and reaches maximum visco-elasticity along with by test, is elevated to higher umber of pulse (each about 470 of reading).Data processing algorithm using initial number as baseline, and reduce all data points by deducting those baseline amount from the data point of all collections, to produce the data and curves (Figure 11) of the value illustrated between 1 and 400, and reached maximum amplitude in 2 minute.Additional test uses " TEG level-2 " sample to perform.According to observations, initial value, slightly lower than those values utilizing level-1 sample to obtain, reads 55 approximately at every turn, and when reaching maximum visco-elasticity, each about 345 of reading.Once application baseline subtraction, then the curve obtained illustrates the value (Figure 12) between 1 and 290, and maximum amplitude detected in 6 minutes.
In the next one research using normal people's whole blood sample, initial baseline umber of pulse typically is low, approximately each reading 80.Reach maximum amplitude along with by test, the high impulse number of acquisition is about 280 pulses of each reading.Also baseline subtraction is applied.The curve obtained illustrates the value (Figure 13) between 1 and 200.Follow-up test uses abnormal people's whole blood sample to perform, and this sample has low blood plasma factor level and low platelet counting compared with normal human blood.According to observations, initial value, slightly lower than those values utilizing normal people's whole blood sample to obtain, reads 65 approximately at every turn.When reaching maximum amplitude, this value reaches each and reads about 165.After deducting baseline, the curve obtained illustrates the value (Figure 14) between 1 and 100.Because whole blood and TEGL1 and L2 sample have different matrix effects, current Setup Experiments not for whole blood sample optimization to reach peak response in this.In these tests, detection head and cuvette are all the disposable elements abandoned after each test separately.
The present invention is with its more high s/n ratio and more high precision and be better than prior art.Compare with pin-cup equipment with those line-cups, it is also robust, also provides wider test specification.In addition, it does not have the time-domain window of the optics gate of inherent noise measurement blood elasticity to eliminate with the change of adhesion properties some problems associated with previous equipment by using in practice.
Present invention utilizes the sensor comprising detection head and rotating disk sub-component.But rotary motion can be limited to the predetermined angular displacement not completing whole rotating range.And, monitor that optical window also can be realized by the other technologies means of such as numerical coding, angle reflection surveying, the comparative analysis of entire motion picture etc. and so on.Other embodiments can, by the combined ability of microcontroller and inner or outer computer in microcontroller or computer, make the use of both be unnecessary.
When making of the present invention, the firmware of microcontroller has (except running actual bioassay) and instructs operator/user by the whole process of execution whole blood test and the task of processing each step of the response/input of user.Also possible that all that step is eliminated to support " key is pressed " process that is full-automatic and that optimize.
Although explain the present invention about its preferred embodiment, should it is easily understood that other possible modifications and variations many can be made when not departing from the spirit and scope of the present invention as described below.
Claims (20)
1. tracing the Magnetic Sensor elasticity measurement equipment (MSED) of mensuration for performing whole blood blood coagulation elasticity for one kind, comprising
Sample cuvette;
Detection head;
The coupling of sensing dish and receiving cavity; And
Rotating disk.
2. the Magnetic Sensor elasticity measurement equipment (MSED) tracing mensuration for performing whole blood blood coagulation elasticity of claim 1, comprises
Optical motion detector;
Optical amplifier and signal gate control circuit;
Heat block;
Dynamo-electric rotating magnetic field generator; And
Block well heater.
3. the Magnetic Sensor elasticity measurement equipment (MSED) tracing mensuration for performing whole blood blood coagulation elasticity of claim 2, comprises
Dish axle; And
Axle stop pin.
4. the Magnetic Sensor elasticity measurement equipment (MSED) tracing mensuration for performing whole blood blood coagulation elasticity of claim 3, comprises
Described sample cuvette and the coaxial orientation of described rotating disk;
Described detection head and described rotating disk are in described sensing dish coupling and receive the coupling of cavity place; And
Described dish axle and described axle stop pin fix described rotating disk.
5. the Magnetic Sensor elasticity measurement equipment (MSED) tracing mensuration for performing whole blood blood coagulation elasticity of claim 3, comprises
Described sample cuvette is disposable;
Described detection head is disposable; And
Described detection head is made up of the plastics spring shape wing.
6. the Magnetic Sensor elasticity measurement equipment (MSED) tracing mensuration for performing whole blood blood coagulation elasticity of claim 3, comprises
Described dynamo-electric rotating magnetic field generator is the dynamo-electric rotating magnetic field generator based on step unit, or
Described dynamo-electric rotating magnetic field generator is the dynamo-electric rotating magnetic field generator based on static coil.
7. the Magnetic Sensor elasticity measurement equipment (MSED) tracing mensuration for performing whole blood blood coagulation elasticity of claim 3, comprises
High speed pulse generator;
Pulse gate control logic;
Microcontroller;
Heater power driving mechanism;
Magnetic power driving mechanism;
Described microprocessor control high-frequency impulse generates; And
Described microprocessor control pulse counting is collected.
8. the Magnetic Sensor elasticity measurement equipment (MSED) tracing mensuration for performing whole blood blood coagulation elasticity of claim 3, comprises
Power supply modulator and distribution;
Microcontroller clock producer;
Communication serial port;
User interface; And
Described user interface comprises lcd screen, hummer and keypad.
9. the Magnetic Sensor elasticity measurement equipment (MSED) tracing mensuration for performing whole blood blood coagulation elasticity of claim 3, comprises
Blood coagulation elasticity traces reagent;
Described blood coagulation elasticity traces the controlled visco-elasticity change that reagent causes blood sample;
It is be selected from one of the group comprising kaolin, tissue factor, heparinase, platelet suppressant drug, Trypsin inhibitor,Trasylol and calcium chloride or more than one that described blood coagulation elasticity traces reagent; And
Described blood coagulation elasticity traces the reagent that reagent is freeze-drying, or
It is air-dry reagent that described blood coagulation elasticity traces reagent.
10. tracing the Magnetic Sensor elasticity measurement equipment (MSED) of mensuration for performing whole blood blood coagulation elasticity for one kind, comprising
Sample cuvette;
Detection head;
The coupling of sensing dish and receiving cavity;
Rotating disk;
Blood coagulation elasticity traces reagent;
Described blood coagulation elasticity traces the controlled visco-elasticity change that reagent causes blood sample;
It is be selected from one of the group comprising kaolin, tissue factor, heparinase, platelet suppressant drug, Trypsin inhibitor,Trasylol and calcium chloride or more than one that described blood coagulation elasticity traces reagent; And
Described blood coagulation elasticity traces the reagent that reagent is freeze-drying, or
It is air-dry reagent that described blood coagulation elasticity traces reagent.
The Magnetic Sensor elasticity measurement equipment (MSED) tracing mensuration for performing whole blood blood coagulation elasticity of 11. claims 10, comprises
Optical motion detector;
Optical amplifier and signal gate control circuit;
Heat block;
Dynamo-electric rotating magnetic field generator;
Block well heater;
Dish axle; And
Axle stop pin.
The Magnetic Sensor elasticity measurement equipment (MSED) tracing mensuration for performing whole blood blood coagulation elasticity of 12. claims 11, comprises
Described sample cuvette and the coaxial orientation of described rotating disk;
Described detection head and described rotating disk are in described sensing dish coupling and receive the coupling of cavity place; And
Described dish axle and described axle stop pin fix described rotating disk.
The Magnetic Sensor elasticity measurement equipment (MSED) tracing mensuration for performing whole blood blood coagulation elasticity of 13. claims 11, comprises
Described sample cuvette is disposable;
Described detection head is disposable; And
Described detection head is made up of the plastics spring shape wing.
The Magnetic Sensor elasticity measurement equipment (MSED) tracing mensuration for performing whole blood blood coagulation elasticity of 14. claims 11, comprises
Described dynamo-electric rotating magnetic field generator is the dynamo-electric rotating magnetic field generator based on step unit, or
Described dynamo-electric rotating magnetic field generator is the dynamo-electric rotating magnetic field generator based on static coil.
The Magnetic Sensor elasticity measurement equipment (MSED) tracing mensuration for performing whole blood blood coagulation elasticity of 15. claims 11, comprises
High speed pulse generator;
Pulse gate control logic;
Microcontroller;
Heater power driving mechanism;
Magnetic power driving mechanism;
Described microprocessor control high-frequency impulse generates; And
Described microprocessor control pulse counting is collected.
The Magnetic Sensor elasticity measurement equipment (MSED) tracing mensuration for performing whole blood blood coagulation elasticity of 16. claims 11, comprises
Power supply modulator and distribution;
Microcontroller clock producer;
Communication serial port;
User interface; And
Described user interface comprises lcd screen, hummer and keypad.
17. 1 kinds of Magnetic Sensor elasticity measurement equipment (MSED) tracing mensuration for performing whole blood blood coagulation elasticity, comprise
Sample cuvette;
Detection head;
The coupling of sensing dish and receiving cavity;
Rotating disk;
Blood coagulation elasticity traces reagent;
Described blood coagulation elasticity traces the controlled visco-elasticity change that reagent causes blood sample;
Described sample cuvette is disposable;
Described detection head is disposable;
Described detection head is made up of the plastics spring shape wing;
It is calcium chloride that described blood coagulation elasticity traces reagent; And
Described blood coagulation elasticity traces the reagent that reagent is freeze-drying, or
It is air-dry reagent that described blood coagulation elasticity traces reagent;
The Magnetic Sensor elasticity measurement equipment (MSED) tracing mensuration for performing whole blood blood coagulation elasticity of 18. claims 17, comprises
Optical motion detector;
Optical amplifier and signal gate control circuit;
Heat block;
Dynamo-electric rotating magnetic field generator;
Block well heater;
Dish axle;
Axle stop pin;
Described sample cuvette and the coaxial orientation of described rotating disk;
Described detection head and described rotating disk are in described sensing dish coupling and receive the coupling of cavity place; And
Described dish axle and described axle stop pin fix described rotating disk.
The Magnetic Sensor elasticity measurement equipment (MSED) tracing mensuration for performing whole blood blood coagulation elasticity of 19. claims 18, comprises
Described dynamo-electric rotating magnetic field generator is the dynamo-electric rotating magnetic field generator based on step unit, or
Described dynamo-electric rotating magnetic field generator is the dynamo-electric rotating magnetic field generator based on static coil.
The Magnetic Sensor elasticity measurement equipment (MSED) tracing mensuration for performing whole blood blood coagulation elasticity of 20. claims 18, comprises
High speed pulse generator;
Pulse gate control logic;
Microcontroller;
Heater power driving mechanism;
Magnetic power driving mechanism;
Described microprocessor control high-frequency impulse generates;
Described microprocessor control pulse counting is collected;
Power supply modulator and distribution;
Microcontroller clock producer;
Communication serial port;
User interface; And
Described user interface comprises lcd screen, hummer and keypad.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261692141P | 2012-08-22 | 2012-08-22 | |
| US61/692,141 | 2012-08-22 | ||
| PCT/US2013/050670 WO2014031253A1 (en) | 2012-08-22 | 2013-07-16 | Device and method for performing blood thromboelastographic assays by magnetic sensing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104981477A true CN104981477A (en) | 2015-10-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201380044224.6A Pending CN104981477A (en) | 2012-08-22 | 2013-07-16 | Device and method for performing blood thromboelastographic assays by magnetic sensing |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP2888274A4 (en) |
| CN (1) | CN104981477A (en) |
| WO (1) | WO2014031253A1 (en) |
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| CN107957380A (en) * | 2018-01-12 | 2018-04-24 | 宝锐生物科技泰州有限公司 | Blood examination probe and blood examination device |
| CN107976382A (en) * | 2018-01-12 | 2018-05-01 | 宝锐生物科技泰州有限公司 | Detection probe and blood examination device |
| CN108508192A (en) * | 2018-04-03 | 2018-09-07 | 江苏锐汗德医疗科技有限公司 | A kind of thrombelastogram detection components and detector |
| CN109072166A (en) * | 2016-04-05 | 2018-12-21 | 通用电气公司 | Activated blood platelet composition with adjustable growth factor levels |
| CN110208511A (en) * | 2019-07-11 | 2019-09-06 | 北京森美希克玛生物科技有限公司 | Elastic force figure instrument and visualization elastic force figure equipment |
| CN110709698A (en) * | 2017-01-26 | 2020-01-17 | 埃尼科尔股份有限公司 | Apparatus and method for measuring viscoelastic changes of a sample |
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| WO2016073668A1 (en) | 2014-11-06 | 2016-05-12 | The Regents Of The University Of Colorado | Identification of novel disease states using viscoelastic analysis in the presence of a thrombolytic agent |
| US11187710B2 (en) | 2015-06-08 | 2021-11-30 | The Regents Of The University Of Colorado, A Body Corporate | Time independent viscoelastic analysis parameter for prediction of patient outcome |
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| CN106771108B (en) * | 2016-12-29 | 2023-06-02 | 山东朗伯光谱设备有限公司 | Method and device for automatically acquiring thromboelastography |
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| CN109072166A (en) * | 2016-04-05 | 2018-12-21 | 通用电气公司 | Activated blood platelet composition with adjustable growth factor levels |
| CN110709698A (en) * | 2017-01-26 | 2020-01-17 | 埃尼科尔股份有限公司 | Apparatus and method for measuring viscoelastic changes of a sample |
| CN110709698B (en) * | 2017-01-26 | 2021-06-01 | 埃尼科尔股份有限公司 | Apparatus and method for measuring viscoelastic changes of a sample |
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| CN107957380A (en) * | 2018-01-12 | 2018-04-24 | 宝锐生物科技泰州有限公司 | Blood examination probe and blood examination device |
| CN107976382A (en) * | 2018-01-12 | 2018-05-01 | 宝锐生物科技泰州有限公司 | Detection probe and blood examination device |
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| CN108508192A (en) * | 2018-04-03 | 2018-09-07 | 江苏锐汗德医疗科技有限公司 | A kind of thrombelastogram detection components and detector |
| CN110208511A (en) * | 2019-07-11 | 2019-09-06 | 北京森美希克玛生物科技有限公司 | Elastic force figure instrument and visualization elastic force figure equipment |
| CN110208511B (en) * | 2019-07-11 | 2024-03-08 | 北京森美希克玛生物科技有限公司 | Elastography instrument and visualized elastography equipment |
Also Published As
| Publication number | Publication date |
|---|---|
| EP2888274A4 (en) | 2016-03-16 |
| WO2014031253A1 (en) | 2014-02-27 |
| EP2888274A1 (en) | 2015-07-01 |
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