CN110192653A - A kind of preparation method of pregnant and lying-in women and children special-purpose probiotics - Google Patents
A kind of preparation method of pregnant and lying-in women and children special-purpose probiotics Download PDFInfo
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- 239000006041 probiotic Substances 0.000 title claims abstract description 59
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 59
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 238000000855 fermentation Methods 0.000 claims abstract description 25
- 230000004151 fermentation Effects 0.000 claims abstract description 23
- 238000004108 freeze drying Methods 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 11
- 238000013329 compounding Methods 0.000 claims abstract description 6
- 238000011081 inoculation Methods 0.000 claims abstract description 6
- 238000001694 spray drying Methods 0.000 claims abstract description 6
- 238000005303 weighing Methods 0.000 claims abstract description 6
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 20
- 239000000843 powder Substances 0.000 claims description 19
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- 229940082509 xanthan gum Drugs 0.000 claims description 18
- 235000010493 xanthan gum Nutrition 0.000 claims description 18
- 239000000230 xanthan gum Substances 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 17
- 241000894006 Bacteria Species 0.000 claims description 13
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 11
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 11
- 241000186604 Lactobacillus reuteri Species 0.000 claims description 11
- 238000007710 freezing Methods 0.000 claims description 11
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- 229940029339 inulin Drugs 0.000 claims description 10
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- 229940035034 maltodextrin Drugs 0.000 claims description 10
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- 239000000725 suspension Substances 0.000 claims description 10
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- 230000000529 probiotic effect Effects 0.000 abstract description 4
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- 102000004169 proteins and genes Human genes 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/147—Helveticus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/173—Reuteri
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of pregnant and lying-in women and the preparation methods of children special-purpose probiotics, include the following steps: weighing, compounding inoculation, fermentation, freeze-drying, primary embedding, secondary embedding, spray drying.Compared with prior art, what the present invention obtained has the beneficial effect that (1) proportion is scientific, full of nutrition, can effectively adjust intestinal health；(2) probiotic microcapsule embedding techniques make probiotics after double wrapped, are resistant to external environment destruction, effectively increase probiotic stability and number of viable.
Description
Technical field
The present invention relates to technical field of health care food, more particularly to a kind of pregnant and lying-in women and children special-purpose probiotics
Preparation method.
Background technique
Microflora in human body intestinal canal can synthesize a variety of growth in humans and develop required vitamin, moreover it is possible to utilize albumen
Matter residue synth essential amino acid, and the metabolism of carbohydrate and protein is participated in, while the mineral elements such as iron, magnesium, zinc can also be promoted
Absorption, these nutriments play a significant role the health of the mankind, once a variety of diseases can be caused by lacking.Except substance synthesizes
Outside metabolic function, the relationship between the microbial ecosystem and body immune system of enteron aisle complexity is also extremely close.Enteron aisle
The immunologic balance that microorganism maintains plays an important role during the prevention of body autoimmune disease, when certain factors cause
When intestinal flora changes, other immune systems of people can be further influenced.
A series of adaptability reconstruct will occur for pregnancy period parent physiological status, and intestinal bacilli illness is to lead to Pregnancy Complication
One of an important factor for, for children since immunity is incomplete, intestinal flora is easy imbalance, causes a series of intestines problems.And
Probiotics can exactly protect stomach health, enhance the anti-virus ability of resistance of human body and human body, and balanced immune is adjusted, prevented
Quick property disease；But after the mankind eat probiotics, the digestion of gastric juice is first passed around, then just can enter in enteron aisle, in gastric juice
The activity of most of probiotics is inhibited even to kill by gastric acid in digestion process, causes probiotics that can not breed in enteron aisle, causes
So that probiotics is lost the possibility being colonized in enteron aisle, reduces its regulating power to pregnant and lying-in women and children's enteron aisle.
Therefore, how to study it is a kind of suitable for pregnant woman and children taking, probiotics that stability is good be art technology
The problem of personnel's urgent need to resolve.
Summary of the invention
In view of this, the present invention provides a kind of pregnant and lying-in women and the preparation methods of children special-purpose probiotics.
To achieve the goals above, the present invention adopts the following technical scheme:
The preparation method of a kind of pregnant and lying-in women and children special-purpose probiotics, includes the following steps:
S1 weighing: 300-800 parts of raspberry juice, 100-120 parts of maltodextrin, 30-50 parts of momordica grosvenori sugar, inulin 10- are weighed
20 parts, 30-50 parts of oligofructose, 3-7 parts of vitamin C, 1-3 parts of lactobacillus acidophilus, 1-3 parts of lactobacillus reuteri and Switzerland's cream
It is 1-3 parts of bacillus, spare；
S2 compounding inoculation: above-mentioned maltodextrin, momordica grosvenori sugar, inulin, oligofructose and vitamin C are added to raspberry juice
In be uniformly mixed, inoculating lactobacillus acidophilus, lactobacillus reuteri and Lactobacillus helveticus, obtain mixed system after sterilization and cooling；
S3 fermentation: above-mentioned mixed system is at the uniform velocity stirred, and fermentation 36-72h must ferment under the conditions of 25-40 DEG C
Liquid；
S4 freeze-drying: Freeze-dry Powder of Probioctics is made in above-mentioned fermentation liquid is concentrated, freeze-drying；
Its activity is difficult to long-term preservation to probiotics in the liquid state, needs to make it into dormant state by being dried, from
And its activity is kept, being made into active bacteria formulation using Freeze Drying Technique is ideal store method.
S5 is once embedded: described Freeze-dry Powder of Probioctics 1-3 parts and xanthan gum solution 3-8 parts being uniformly mixed, pH value is adjusted
To 5.0, mixed liquor is made, 1 part of mixed liquor is added dropwise in 2-5 parts of chitosan solutions, 30-50min, mistake are continuously stirred
It filters, substance is retained on collection membrane, abandon filtrate, retained substance 3 times on sterilized normal saline flushing film, once embedded
Probiotics；
Chitosan and xanthan gum are natural large biological molecules, and good biocompatibility has no toxic side effect, and chitosan is dissolved in acid
Property aqueous solution in, amino acid is positively charged in an acidic solution, can pass through with negatively charged anion polysaccharide xanthan gum poly-
Electrolyte complexing forms cogelled, uses as wall material.
S6 bis- times embeddings: primary embedding probiotics is placed in the xanthan gum solution of concentration 0.2%, 20- is persistently stirred
40min obtains secondary embedding probiotics；
It is once embedded using xanthan gum and chitosan, the porous gel reticular structure formed can not be fully effective
Limitation small molecule harmful substance infiltration, keep it very limited to the protective effect of probiotics, using secondary embedding techniques, energy
Increase probiotics to the resilience of external environment, improves its stability and vigor.
S7 spray drying: secondary embedding probiotics is spray-dried up to pregnant and lying-in women and children special-purpose probiotics.
Preferably, the concrete operations process being freeze-dried in step S4 are as follows:
S41: the fermentation liquid after concentration is uniformly mixed with the skimmed milk powder that concentration is 3%, bacteria suspension, the concentration is made
The volume ratio of post-fermentation liquid and the skimmed milk powder is 1: 3；
S42: by bacteria suspension in -60 DEG C of precooling 5h, later in -20 DEG C of freezings 4h, 0 DEG C of freezing 2h, 10 DEG C of dry 2h, most
25 DEG C of dry 1h afterwards.
During vacuum refrigeration, the concentration of intracellular cytosol or the growth of ice crystal easily cause cell damage, the present invention
Technical solution joined protective agent skimmed milk powder in freeze-drying, reduces damage death of the thallus in freezing dry process, improves
Thallus survival rate.
Preferably, chitosan solution concentration described in step S5 is 0.4-0.8%, and the xanthan gum solution concentration is 0.3-
0.8%.
Preferably, 120-150 DEG C of inlet air temperature is controlled in step S7, sample introduction flow 700-800ml/h, atomisation pressure 0.1-
0.3MPa。
It can be seen via above technical scheme that compared with prior art, what the present invention obtained has the beneficial effect that (1) is prebiotic
Microbial inoculum proportion is scientific, full of nutrition, can effectively adjust intestinal health；(2) probiotics can be resisted and be resistant to and is outer after double wrapped
Boundary's environmental disruption, effectively increases probiotic stability and number of viable.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
Microorganism raw material sources in embodiment:
Lactobacillus acidophilus, CAS number: 2623, it is purchased from Xi'an Heng Ji Chemical Co., Ltd.；
Lactobacillus reuteri CICC6226 is purchased from Guangzhou Wei Jia Science and Technology Ltd.；
Lactobacillus helveticus CICC 20243 is purchased from Beijing Yu Wei Science and Technology Ltd..
Embodiment 1
S1 weighing: raspberry juice 300kg, maltodextrin 100kg, momordica grosvenori sugar 50kg, inulin 10kg, oligofructose are weighed
50kg, vitamin C 3kg, lactobacillus acidophilus 1kg, lactobacillus reuteri 3kg and Lactobacillus helveticus 3kg, it is spare；
S2 compounding inoculation: above-mentioned maltodextrin, momordica grosvenori sugar, inulin, oligofructose and vitamin C are added to raspberry juice
In be uniformly mixed, inoculating lactobacillus acidophilus, lactobacillus reuteri and Lactobacillus helveticus, obtain mixed system after sterilization and cooling；
S3 fermentation: above-mentioned mixed system is at the uniform velocity stirred, and fermentation 72h obtains fermentation liquid under the conditions of 25 DEG C；
S4 freeze-drying: the fermentation liquid after concentration is uniformly mixed at 1: 3 by volume with the skimmed milk powder that concentration is 3%, is made
Bacteria suspension；By bacteria suspension in -60 DEG C of precooling 5h, later in -20 DEG C of freezings 4h, 0 DEG C of freezing 2h, 10 DEG C of dry 2h, last 25
DEG C dry 1h, obtains Freeze-dry Powder of Probioctics；
S5 is once embedded: the Freeze-dry Powder of Probioctics 1kg and concentration the xanthan gum solution 3kg for being 0.3% is uniformly mixed,
PH value is adjusted to 5.0, mixed liquor is made, mixed liquor 1kg is added dropwise in the chitosan solution that 2kg concentration is 0.8%, even
Continuous stirring 30min, filters, substance is retained on collection membrane, abandon filtrate, with retention substance 3 on sterilized normal saline flushing film
It is secondary, it obtains once embedding probiotics；
S6 bis- times embeddings: primary embedding probiotics is placed in the xanthan gum solution of concentration 0.2%, probiotics is once embedded
It is 1: 5 with 0.2% xanthan gum solution volume ratio of concentration, persistently stirs 20min, obtain secondary embedding probiotics；
S7 spray drying: secondary embedding probiotics is spray-dried, and controls 120 DEG C of inlet air temperature, sample introduction flow
700ml/h, atomisation pressure 0.3MPa are to get pregnant and lying-in women and children special-purpose probiotics.
Embodiment 2
S1 weighing: raspberry juice 800kg, maltodextrin 120kg, momordica grosvenori sugar 30kg, inulin 20kg, oligofructose are weighed
30kg, vitamin C 7kg, lactobacillus acidophilus 3kg, lactobacillus reuteri 1kg and Lactobacillus helveticus 1kg, it is spare；
S2 compounding inoculation: above-mentioned maltodextrin, momordica grosvenori sugar, inulin, oligofructose and vitamin C are added to raspberry juice
In be uniformly mixed, inoculating lactobacillus acidophilus, lactobacillus reuteri and Lactobacillus helveticus, obtain mixed system after sterilization and cooling；
S3 fermentation: above-mentioned mixed system is at the uniform velocity stirred, and fermentation 36h obtains fermentation liquid under the conditions of 40 DEG C；
S4 freeze-drying: the fermentation liquid after concentration is uniformly mixed at 1: 3 by volume with the skimmed milk powder that concentration is 3%, is made
Bacteria suspension；By bacteria suspension in -60 DEG C of precooling 5h, later in -20 DEG C of freezings 4h, 0 DEG C of freezing 2h, 10 DEG C of dry 2h, last 25
DEG C dry 1h, obtains Freeze-dry Powder of Probioctics；
S5 is once embedded: the Freeze-dry Powder of Probioctics 3kg and concentration the xanthan gum solution 8kg for being 0.8% is uniformly mixed,
PH value is adjusted to 5.0, mixed liquor is made, mixed liquor 1kg is added dropwise in the chitosan solution that 5kg concentration is 0.4%, even
Continuous stirring 50min, filters, substance is retained on collection membrane, abandon filtrate, with retention substance 3 on sterilized normal saline flushing film
It is secondary, it obtains once embedding probiotics；
S6 bis- times embeddings: primary embedding probiotics is placed in the xanthan gum solution of concentration 0.2%, probiotics is once embedded
It is 1: 5 with 0.2% xanthan gum solution volume ratio of concentration, persistently stirs 40min, obtain secondary embedding probiotics；
S7 spray drying: secondary embedding probiotics is spray-dried, and controls 150 DEG C of inlet air temperature, sample introduction flow
800ml/h, atomisation pressure 0.1MPa are to get pregnant and lying-in women and children special-purpose probiotics.
Embodiment 3
S1 weighing: raspberry juice 500kg, maltodextrin 110kg, momordica grosvenori sugar 40kg, inulin 15kg, oligofructose are weighed
40kg, vitamin C 5kg, lactobacillus acidophilus 2kg, lactobacillus reuteri 2kg and Lactobacillus helveticus 2kg, it is spare；
S2 compounding inoculation: above-mentioned maltodextrin, momordica grosvenori sugar, inulin, oligofructose and vitamin C are added to raspberry juice
In be uniformly mixed, inoculating lactobacillus acidophilus, lactobacillus reuteri and Lactobacillus helveticus, obtain mixed system after sterilization and cooling；
S3 fermentation: above-mentioned mixed system is at the uniform velocity stirred, and fermentation 48h obtains fermentation liquid under the conditions of 37 DEG C；
S4 freeze-drying: the fermentation liquid after concentration is uniformly mixed at 1: 3 by volume with the skimmed milk powder that concentration is 3%, is made
Bacteria suspension；By bacteria suspension in -60 DEG C of precooling 5h, later in -20 DEG C of freezings 4h, 0 DEG C of freezing 2h, 10 DEG C of dry 2h, last 25
DEG C dry 1h, obtains Freeze-dry Powder of Probioctics；
S5 is once embedded: the Freeze-dry Powder of Probioctics 2kg and concentration the xanthan gum solution 6kg for being 0.5% is uniformly mixed,
PH value is adjusted to 5.0, mixed liquor is made, mixed liquor 1kg is added dropwise in the chitosan solution that 3kg concentration is 0.6%, even
Continuous stirring 40min, filters, substance is retained on collection membrane, abandons filtrate and is once wrapped with sterilized normal saline flushing 3 times
Bury probiotics；
S6 bis- times embeddings: primary embedding probiotics is placed in the xanthan gum solution of concentration 0.2%, probiotics is once embedded
It is 1: 5 with 0.2% xanthan gum solution volume ratio of concentration, persistently stirs 30min, obtain secondary embedding probiotics；
S7 spray drying: secondary embedding probiotics is spray-dried, and controls 130 DEG C of inlet air temperature, sample introduction flow
750ml/h, atomisation pressure 0.2MPa are to get pregnant and lying-in women and children special-purpose probiotics.
Comparative example 1
Concentration post-fermentation liquid is not mixed with skimmed milk powder and directly carries out frozen dried, remaining operation is the same as embodiment 3.
Comparative example 2
It is directly spray-dried after once embedding, without secondary embedding treatment, remaining operation is the same as embodiment 3.
Comparative example 3
Subsequent operation is directly carried out without freeze-drying after fermentation liquid concentration, remaining step is the same as embodiment 3.
Test 1 survival assays
It is prepared using embodiment 1, embodiment 2, embodiment 3, comparative example 1, comparative example 2, the technical solution of comparative example 3 prebiotic
Bacteria agent, and survival assays are carried out, test method is as follows: 0.5g probiotics microbial inoculum is added in the SGJ of 4.5ml (37 DEG C
Preheating), 10s fullys shake with turbula shaker, 2h is then incubated under 37 DEG C, 100r/min, uses high speed homogenization device pair later
Sample solution is crushed, and with ULTRA-TURRAX T8 homogenizer, spread plate is used in homogeneous 45s, sampling at 10000r/min
Method measures viable count, calculates survival rate (SY), and the calculation formula of survival rate (SY) is as follows:
SY (%)=N/N0× 100%；
N is the total number of cells of survival after treatment；
N0For cell total viable count before treatment.
Test result is as shown in table 1.
The survival rate of the different preparation method probiotics of table 1
| Processing | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 |
| Survival rate (%) | 74.6 | 76.8 | 80.5 | 54.3 | 62.8 | 49.6 |
Test result shows that the survival rate of the probiotics of the method for the present invention preparation is significantly larger than control group.
The pregnant mouse Immune Function test of 2 pairs of test
(1) experimental animal grouping and administration
It chooses age of mouse 2 months, the Kunming mouse of 20 ± 2g of weight, female mice and male mouse are with the same cage of 2: 1 ratios, the inspection of next day smear
Female mice vaginal fluid is looked into, gestation is set to if finding sperm the 0th.Pregnant mouse is randomly divided into 5 groups, wherein 1 group of blank group,
Test group is divided into 4 groups, and probiotic composition used in test group respectively corresponds embodiment 3, comparative example 1, comparative example 2 and comparative example 3,
Every group of pregnant mouse 20.Blank group gives 37 DEG C of warm water stomach-fillings 3 times daily；Test group uses embodiment 3, comparative example 1, comparison daily
Probiotics solution stomach-filling 3 times described in example 2 and comparative example 3, solution dosage are 100mg/kg, each stomach-filling 0.5ml.
(2) pregnant mouse peripheral blood immunocyte and peritoneal macrophage measurement
Pregnant mouse gestation the 18th day, eyeball is won, whole peripheral bloods (using mouse haemorrhagic shock as standard) is acquired, uses
Tris-NH4After the hypotonic destruction red blood cell of CI solution, immunocytochemistry is carried out.Then on clean bench after dissection blood sampling
Pregnant mouse, take peritoneal macrophage to be counted.Test result is as shown in table 2.
The pregnant mouse peripheral blood immunocyte of table 2 and peritoneal macrophage measurement result
The immune function that test result shows to take pregnant mouse after probiotics of the present invention increases, and peripheral blood is immune thin
Born of the same parents' quantity and peritoneal macrophage quantity are obviously improved.
Test the influence test of 3 pairs of mouse chow utilization rates
(1) experimental animal and grouping: weight is kind female mice 100, the SPF grade Kunming of 18g-22g, is randomly divided into 5
Group, wherein 1 group of control group, test group are divided into 4 groups, and probiotics used in test group respectively corresponds embodiment 3, comparative example 1, comparative example
2 and comparative example 3, control group is with isodose starch feeding.
(2) test method: entire test period is 8 weeks, wherein daily each group stomach-filling gives corresponding dosage tested material 2 weeks,
It observes again later 6 weeks.Each group mouse freely ingests and drinks water during test, and feeding mouse growth breeds pellet, claims weekly
Amount 1 time measures the food-intake of groups of animals daily, calculates each group mouse chow utilization rate.
Food utilization=body weight increase amount/food weight × 100%.
Test result is as shown in table 3.
3 mouse chow utilization rate measurement result of table
Test result shows that mouse chow utilization rate can be improved in probiotics of the invention.
Each embodiment in this specification is described in a progressive manner, the highlights of each of the examples are with other
The difference of embodiment, the same or similar parts in each embodiment may refer to each other.For device disclosed in embodiment
For, since it is corresponded to the methods disclosed in the examples, so description is relatively simple, related place is said referring to method part
It is bright.
The general principles defined herein can without departing from the spirit or scope of the present invention, in other realities
It applies in example and realizes.Therefore, the present invention is not intended to be limited to embodiment illustrated herein, and be to fit to it is disclosed herein
Principle and the consistent widest scope of features of novelty.
Claims (4)
1. the preparation method of a kind of pregnant and lying-in women and children special-purpose probiotics, which comprises the steps of:
S1 weighing: weigh 300-800 parts of raspberry juice, 100-120 parts of maltodextrin, 30-50 parts of momordica grosvenori sugar, 10-20 parts of inulin,
30-50 parts of oligofructose, 3-7 parts of vitamin C, 1-3 parts of lactobacillus acidophilus, 1-3 parts of lactobacillus reuteri and Lactobacillus helveticus 1-
It is 3 parts, spare；
S2 compounding inoculation: above-mentioned maltodextrin, momordica grosvenori sugar, inulin, oligofructose and vitamin C are added in raspberry juice and mixed
It closes uniformly, inoculating lactobacillus acidophilus, lactobacillus reuteri and Lactobacillus helveticus, obtain mixed system after sterilization and cooling；
S3 fermentation: above-mentioned mixed system is at the uniform velocity stirred, and the 36-72h that ferments under the conditions of 25-40 DEG C, obtains fermentation liquid；
S4 freeze-drying: Freeze-dry Powder of Probioctics is made in above-mentioned fermentation liquid is concentrated, freeze-drying；
S5 is once embedded: described Freeze-dry Powder of Probioctics 1-3 parts and xanthan gum solution 3-8 part are uniformly mixed, adjusting pH value to
5.0, mixed liquor is made；1 part of mixed liquor is added dropwise in 2-5 parts of chitosan solutions, 30-50min is continuously stirred, is filtered,
Substance is retained on collection membrane, filtrate is abandoned, is retained substance 3 times on sterilized normal saline flushing film, obtains once embedding benefit
Raw bacterium；
S6 bis- times embeddings: primary embedding probiotics being placed in the xanthan gum solution of concentration 0.2%, 20-40min is persistently stirred,
Obtain secondary embedding probiotics；
S7 spray drying: secondary embedding probiotics is spray-dried up to pregnant and lying-in women and children special-purpose probiotics.
2. the preparation method of a kind of pregnant and lying-in women as described in claim 1 and children special-purpose probiotics, which is characterized in that step S4
The detailed process of middle freeze-drying includes:
S41: the fermentation liquid after concentration is uniformly mixed with the skimmed milk powder that concentration is 3%, bacteria suspension is made, after the concentration
The volume ratio of fermentation liquid and the skimmed milk powder is 1: 3；
S42: by bacteria suspension in -60 DEG C of precooling 5h, later in -20 DEG C of freezings 4h, 0 DEG C of freezing 2h, 10 DEG C of dry 2h, last 25
DEG C dry 1h.
3. the preparation method of a kind of pregnant and lying-in women as described in claim 1 and children special-purpose probiotics, which is characterized in that step S5
Described in chitosan solution concentration be 0.4-0.8%, the xanthan gum solution concentration be 0.3-0.8%.
4. the preparation method of a kind of pregnant and lying-in women as described in claim 1 and children special-purpose probiotics, which is characterized in that step S7
120-150 DEG C of inlet air temperature of middle control, sample introduction flow 700-800ml/h, atomisation pressure 0.1-0.3MPa.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110638845A (en) * | 2019-11-08 | 2020-01-03 | 南昌诺汇医药科技有限公司 | Bacterium agent composition and application thereof |
| CN113521013A (en) * | 2020-04-20 | 2021-10-22 | 青岛海益康健康科技有限公司 | Embedded probiotic product with planting effect and preparation process thereof |
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2019
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110638845A (en) * | 2019-11-08 | 2020-01-03 | 南昌诺汇医药科技有限公司 | Bacterium agent composition and application thereof |
| CN113521013A (en) * | 2020-04-20 | 2021-10-22 | 青岛海益康健康科技有限公司 | Embedded probiotic product with planting effect and preparation process thereof |
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Application publication date: 20190903 |