CN110192096A - 生物体物质检测用设备和检测装置、离子电流的测定方法以及生物体物质的识别方法 - Google Patents
生物体物质检测用设备和检测装置、离子电流的测定方法以及生物体物质的识别方法 Download PDFInfo
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Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102000658A (zh) * | 2010-12-15 | 2011-04-06 | 西南交通大学 | 一种基于聚多巴胺的生物功能化改性方法 |
WO2013137209A1 (ja) * | 2012-03-13 | 2013-09-19 | 株式会社 東芝 | 一粒子解析装置および解析方法 |
US20130344229A1 (en) * | 2006-10-19 | 2013-12-26 | Northwestern University | Surface-independent, surface-modifying, multifunctional coatings and applications thereof |
CN104011077A (zh) * | 2011-09-30 | 2014-08-27 | 赛特瑞恩股份有限公司 | 从人b细胞产生的具有甲型流感病毒中和活性的结合分子 |
US20140255911A1 (en) * | 2013-03-07 | 2014-09-11 | Kabushiki Kaisha Toshiba | Sample detection apparatus and detection method |
US20140374695A1 (en) * | 2013-06-19 | 2014-12-25 | International Business Machines Corporation | Nanogap device with capped nanowire structures |
US20150204873A1 (en) * | 2012-06-18 | 2015-07-23 | Electonic Biosciences, Inc. | Cell-free assay device and methods of use |
US20150377830A1 (en) * | 2014-06-26 | 2015-12-31 | International Business Machines Corporation | Detection of translocation events using graphene-based nanopore assemblies |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3915849B2 (ja) * | 1997-05-09 | 2007-05-16 | 雪印乳業株式会社 | シアリルラクトース結合ポリスチレン誘導体及びその用途 |
US6171578B1 (en) * | 1999-04-14 | 2001-01-09 | Diatide, Inc. | Benzodiazepine derivatives for imaging thrombi |
US10006879B2 (en) * | 2011-04-12 | 2018-06-26 | Electronic Biosciences, Inc. | Site specific chemically modified nanopore devices |
CN104066850B (zh) | 2011-09-23 | 2017-11-10 | 牛津楠路珀尔科技有限公司 | 包含聚合物单元的聚合物的分析 |
JP2013090576A (ja) | 2011-10-24 | 2013-05-16 | Hitachi Ltd | 核酸分析デバイス及びそれを用いた核酸分析装置 |
WO2013125225A1 (en) * | 2012-02-23 | 2013-08-29 | Shimoide Yoshiteru | Composition for preventing or treating allergic disease or major depressive disorder (depression) |
US20130256139A1 (en) * | 2012-03-30 | 2013-10-03 | International Business Machines Corporation | Functionalized graphene or graphene oxide nanopore for bio-molecular sensing and dna sequencing |
JP2014210245A (ja) * | 2013-04-22 | 2014-11-13 | 株式会社日立ハイテクノロジーズ | ナノポア作製方法、ナノポア作製装置、及びナノポアを用いた分析装置 |
JP6189231B2 (ja) | 2014-02-26 | 2017-08-30 | 株式会社東芝 | 微粒子検査装置と微粒子検査システム及び微粒子検査方法 |
WO2016077263A1 (en) * | 2014-11-12 | 2016-05-19 | Arizona Board Of Regents On Behalf Of Arizona State University | System and method for nucleotide sequencing |
US9551707B2 (en) * | 2015-04-30 | 2017-01-24 | International Business Machines Corporation | Gold ion beam drilled nanopores modified with thiolated DNA origamis |
-
2017
- 2017-04-21 JP JP2018513228A patent/JP6638913B2/ja active Active
- 2017-04-21 CN CN201780024754.2A patent/CN110192096B/zh active Active
- 2017-04-21 US US16/095,340 patent/US10877021B2/en active Active
- 2017-04-21 WO PCT/JP2017/016041 patent/WO2017183716A1/ja active Application Filing
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20130344229A1 (en) * | 2006-10-19 | 2013-12-26 | Northwestern University | Surface-independent, surface-modifying, multifunctional coatings and applications thereof |
CN102000658A (zh) * | 2010-12-15 | 2011-04-06 | 西南交通大学 | 一种基于聚多巴胺的生物功能化改性方法 |
CN104011077A (zh) * | 2011-09-30 | 2014-08-27 | 赛特瑞恩股份有限公司 | 从人b细胞产生的具有甲型流感病毒中和活性的结合分子 |
WO2013137209A1 (ja) * | 2012-03-13 | 2013-09-19 | 株式会社 東芝 | 一粒子解析装置および解析方法 |
US20150204873A1 (en) * | 2012-06-18 | 2015-07-23 | Electonic Biosciences, Inc. | Cell-free assay device and methods of use |
US20140255911A1 (en) * | 2013-03-07 | 2014-09-11 | Kabushiki Kaisha Toshiba | Sample detection apparatus and detection method |
US20140374695A1 (en) * | 2013-06-19 | 2014-12-25 | International Business Machines Corporation | Nanogap device with capped nanowire structures |
US20150377830A1 (en) * | 2014-06-26 | 2015-12-31 | International Business Machines Corporation | Detection of translocation events using graphene-based nanopore assemblies |
Non-Patent Citations (1)
Title |
---|
张巧玲 等: "《化工工艺学》", 31 July 2015, 国防工业出版社 * |
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