CN110150332B - Carvacrol-containing composition, bactericide, preparation method and application thereof - Google Patents
Carvacrol-containing composition, bactericide, preparation method and application thereof Download PDFInfo
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- CN110150332B CN110150332B CN201910584167.XA CN201910584167A CN110150332B CN 110150332 B CN110150332 B CN 110150332B CN 201910584167 A CN201910584167 A CN 201910584167A CN 110150332 B CN110150332 B CN 110150332B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
Abstract
The invention provides a carvacrol-containing composition, a bactericide, a preparation method and an application thereof, and relates to the technical field of pesticides. The composition containing carvacrol comprises carvacrol and chrysanthemum indicum essential oil, wherein the volume ratio of the carvacrol to the chrysanthemum indicum essential oil is (1-10): (10-1). According to the plant source composition provided by the invention, carvacrol and chrysanthemum indicum essential oil are compounded, so that the synergistic effect is achieved, and the plant source composition has good control effects on tobacco bacterial wilt and black shank. The bactericide prepared by the composition has the prevention effect on tobacco bacterial wilt of more than 50 percent and the prevention effect on tobacco black shank of more than 65 percent, and has comprehensive prevention and control effect on two diseases.
Description
Technical Field
The invention relates to the technical field of pesticides, and particularly relates to a carvacrol-containing composition, a bactericide, a preparation method and an application thereof.
Background
Bacterial wilt of plants caused by Laurella solanacearum is a worldwide disease widely distributed in tropical, subtropical and some temperate regions, is a main cause of yield reduction of various crops, and is particularly more harmful to tobacco. The bacterial wilt is spread rapidly and has wide host range, so that it is very difficult to prevent and cure, so that it is called "plant cancer".
Phytophthora nicotianae, a typical soil-borne pathogen, is now considered to be one of the most devastating oomycete diseases, and is capable of infecting over 255 plants, including severely compromised tobacco (Nicotiana spp.), tomatoes (Lycopersicum esculentum), Citrus (Citrus spp.), and the like, and is particularly responsible for the severe black shank hazard on tobacco. Due to the diversity of hosts and the wide distribution of ecological regions, the huge loss caused by the hosts is difficult to estimate and is a continuous challenge for controlling plant diseases.
The prevention and control of the tobacco bacterial wilt mainly depend on agricultural streptomycin sulfate, and along with the production stoppage of agricultural streptomycin sulfate in China, a substitute medicament is urgently needed in production. The existing prevention and treatment agent is mainly a copper agent, has poor prevention and treatment effect and pollutes the environment. The tobacco black shank prevention and control mainly comprises chemical agents such as metalaxyl, dimethomorph and the like, and is easy to cause negative effects such as pesticide residue, environmental pollution, pathogenic bacteria drug resistance and the like. Because the two diseases are frequently mixed in the field, the pesticide with the control effect on the two diseases is lacked in production, and a single pesticide is only effective on one disease, so that the control effect is poor, and the pesticide application cost is high.
Disclosure of Invention
The invention aims to provide a carvacrol-containing composition, a bactericide, a preparation method and an application thereof. The composition provided by the invention has good control effects on tobacco bacterial wilt and black shank. The bactericide prepared by the composition has the control effect on tobacco bacterial wilt of more than 50 percent and the control effect on tobacco black shank of more than 65 percent.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a carvacrol-containing composition which comprises carvacrol and chrysanthemum indicum essential oil, wherein the volume ratio of the carvacrol to the chrysanthemum indicum essential oil is (1-10) to (10-1).
Preferably, the volume ratio of the carvacrol to the wild chrysanthemum essential oil is (7-9): (3-2).
The invention also provides a bactericide comprising the composition of the technical scheme, which is prepared from the following raw materials in parts by mass: 15-25 parts of the composition, 3.5-6.5 parts of an emulsifier, 1.5-2.5 parts of a co-emulsifier, 2-4 parts of an anti-freezing agent, 0.5-1.5 parts of an antioxidant and 51-87 parts of water.
Preferably, the emulsifier comprises one or more of polyether phosphate, phenol ether phosphate, phenethyl phenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene, nonylphenol polyoxyethylene ether phosphate and calcium dodecylbenzene sulfonate.
Preferably, the co-emulsifier comprises one or more of n-butanol, n-hexanol and tridecanol.
Preferably, the antifreeze agent comprises ethylene glycol and/or glycerol.
Preferably, the antioxidant comprises dibutylhydroxytoluene.
The invention also provides application of the composition in the technical scheme in preventing and treating bacterial wilt diseases.
The invention also provides application of the composition in the technical scheme in prevention and treatment of black shank diseases.
The invention also provides application of the bactericide in the technical scheme in preventing and treating bacterial wilt and/or black shank diseases.
The invention provides a carvacrol-containing composition, a bactericide, a preparation method and an application thereof. The composition provided by the invention has a synergistic effect by compounding the carvacrol and the chrysanthemum indicum essential oil, and has good control effects on tobacco bacterial wilt and black shank. The bactericide prepared by the composition has the prevention effect on tobacco bacterial wilt of more than 50 percent and the prevention effect on tobacco black shank of more than 65 percent, and has comprehensive prevention and control effect on two diseases.
Detailed Description
The invention provides a carvacrol-containing composition which comprises carvacrol and chrysanthemum indicum essential oil, wherein the volume ratio of the carvacrol to the chrysanthemum indicum essential oil is (1-10) - (10-1), and preferably (7-9) - (3-2). The sources of the carvacrol and the wild chrysanthemum essential oil are not particularly limited, and the pure products sold in the market are adopted.
The invention also provides a bactericide comprising the composition of the technical scheme, which is prepared from the following raw materials in parts by mass: 15-25 parts of the composition, 3.5-6.5 parts of an emulsifier, 1.5-2.5 parts of a co-emulsifier, 2-4 parts of an anti-freezing agent, 0.5-1.5 parts of an antioxidant and 51-87 parts of water.
The bactericide provided by the invention comprises 15-25 parts by mass of the composition, and preferably 20 parts by mass of the composition. In the invention, the composition has control effects on tobacco bacterial wilt and black shank.
The bactericide provided by the invention comprises 3.5-6.5 parts by mass of an emulsifier, preferably 5 parts by mass. The type of the emulsifier is not particularly limited, and one or more of polyether phosphate, phenol ether phosphate, phenethyl phenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene, nonylphenol polyoxyethylene ether phosphate and calcium dodecylbenzene sulfonate are preferably selected. The source of the emulsifier is not particularly limited in the invention, and the emulsifier can be obtained by adopting a conventional commercial product. In the present invention, the emulsifier has the effects of emulsification, wetting, dispersion, viscosity adjustment, and the like.
The bactericide provided by the invention comprises 1.5-2.5 parts by mass of co-emulsifier, and preferably 2 parts by mass. The kind of the co-emulsifier is not particularly limited in the present invention, and one or more of n-butanol, n-hexanol and tridecanol are preferred. The source of the co-emulsifier is not particularly limited in the invention, and the co-emulsifier can be obtained by adopting a conventional commercial product. In the invention, the co-emulsifier has the functions of adjusting the hydrophilic-lipophilic balance value of the emulsifier and the like.
The bactericide provided by the invention comprises 2-4 parts by mass of an antifreezing agent, and preferably 3 parts by mass. In the present invention, the kind of the antifreeze is not particularly limited, and ethylene glycol and/or glycerin are preferable. The source of the antifreeze is not particularly limited, and the antifreeze can be obtained by adopting a conventional commercial product. In the invention, the antifreezing agent has the functions of improving the disease resistance, low temperature resistance and freezing resistance of crops.
The bactericide provided by the invention comprises 0.5-1.5 parts by mass of antioxidant, and preferably 1 part by mass. In the present invention, the kind of the antioxidant is not particularly limited, and dibutylhydroxytoluene is preferable. The source of the antioxidant is not particularly limited in the present invention, and a conventional commercially available product may be used. In the present invention, the antioxidant has an antioxidant effect.
The invention also provides a preparation method of the bactericide in the technical scheme, which preferably comprises the following steps: mixing the emulsifier, the co-emulsifier, the antifreeze, the antioxidant and water to obtain a water phase; and mixing the composition with the obtained water to obtain the bactericide. The order of mixing the composition and the aqueous phase is not particularly limited in the present invention, and the composition may be added to the aqueous phase or the aqueous phase may be added to the composition. The mixing mode is not particularly limited, a shear homogenizing mixing method is preferably adopted, and the mixing time is preferably 25-35 min.
The invention also provides application of the composition in the technical scheme in preventing and treating bacterial wilt diseases. In the present invention, the bacterial wilt disease is preferably tobacco bacterial wilt.
The invention also provides application of the composition in the technical scheme in prevention and treatment of black shank diseases. In the invention, the black shank disease is preferably tobacco black shank.
The invention also provides application of the bactericide in the technical scheme in preventing and treating bacterial wilt and black shank diseases.
In the invention, when the bactericide is used for preventing and treating tobacco bacterial wilt, 500 times of liquid is preferably used for root irrigation, 100mL of the bactericide is applied per plant, more preferably, the bactericide is applied 1 month after transplantation, and the bactericide is continuously applied for 2-3 times at intervals of 7-10 days.
In the invention, when the bactericide is used for preventing and treating tobacco black shank, 500 times of liquid is preferably used for stem drenching and root irrigation, 100mL of the bactericide is applied per plant, more preferably, the bactericide is continuously applied for 2-3 times when sporadic plants appear in the field, and the interval is 7-10 days each time.
The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The preparation method of the bactericide comprises the following steps: mixing 5g of polyether phosphate, 2g of n-butanol, 3g of ethylene glycol, 1g of butylated hydroxytoluene and 69g of water to prepare a water phase, adding the water phase into 20g of plant source composition containing carvacrol under the condition of high-speed shearing, and shearing and homogenizing for 30min to obtain the bactericide. Wherein the mass of carvacrol in the composition is 18g, and the mass of chrysanthemum indicum essential oil is 2 g; the emulsification adopts a conventional high-speed shearing emulsifying machine for emulsification.
Example 2
The preparation method of the bactericide comprises the following steps: 5g of phenol ether phosphate, 2g of tridecanol, 3g of glycerol, 1g of butylated hydroxytoluene and 69g of water are mixed to prepare a water phase, and the water phase is added into 20g of plant source composition containing carvacrol under the condition of high-speed shearing, and is sheared and homogenized for 30min to obtain the bactericide. Wherein the mass of carvacrol in the composition is 16g, and the mass of chrysanthemum indicum essential oil is 4 g; the emulsification adopts a conventional high-speed shearing emulsifying machine for emulsification.
Comparative example 1
Determination of antibacterial activity of carvacrol
Materials and methods
1.1 test strains
Tobacco bacterial wilt pathogen (Ralstonia solanacearum) and tobacco black shank (Phytophthora nicotianae).
1.2 Activity of carvacrol in inhibiting Ralstonia solanacearum
Minimum Inhibitory Concentration (MIC) assay. The antibacterial activity of carvacrol on ralstonia solanacearum is measured on a 96-hole cell culture plate. Washing ralstonia solanacearum activated for 12h on NA slant culture medium with sterile water, and diluting to bacteria content of 108CFU/mL, respectively adding 190 μ L of ralstonia solanacearum into 10 μ L of culture wells containing carvacrol with final concentrations of 50, 100, 150, 180 and 200 μ L/L, and performing constant temperature shaking culture at 150r/min and 30 ℃ for 24h, wherein the minimum inhibitory concentration is defined as the concentration at which ralstonia solanacearum cannot grow.
Determination of concentration in inhibition. The antibacterial activity of carvacrol on ralstonia solanacearum is measured on a 96-well cell culture plate. The ralstonia solanacearum activated for 12h on the NA slant culture medium is washed by sterile water, diluted to the bacterial content of about 108CFU/mL, respectively added into culture wells containing carvacrol with final concentrations of 10, 20, 40, 60, 80, 100 and 120 mul/L respectively, subjected to shaking culture at the constant temperature of 30 ℃ for 24h at 150r/min, and the absorbance value of the bacterial liquid is measured at 600 nm. The control was the inoculum without eugenol, and 3 replicates of each treatment were used. EC50 values were calculated.
1.3 Cochinol inhibits the activity of Heiyiphora nigra
A hypha growth rate method is adopted. Carvacrol was plated in DMSO (0.5%, v/v) at various concentrations on conventional oat medium (at about45 ℃) to final concentrations of 20, 25, 30, 35, 40 and 45. mu.L/L. Inoculating phytophthora nicotianae cakes (5mm) to a conventional oat culture medium containing carvacrol at different concentrations, culturing at 28 ℃ for 5 days, taking DMSO with the same dilution ratio as a control, measuring the colony diameter by a cross method, and calculating the hypha growth inhibition rate and the EC50 value.
Results and analysis
2.1 inhibiting the activity of tobacco Ralstonia solanacearum by carvacrol
The MIC determination result shows that no ralstonia solanacearum growth is found in the carvacrol treated at the concentration higher than 180 mul/L, which indicates that the MIC of carvacrol to ralstonia solanacearum is 180 mul/L. As shown in Table 1, carvacrol has a good inhibition effect on tobacco ralstonia solanacearum, the toxicity equation is that y is 1.3483+2.4634x, R2 is 0.9922, the EC50 value is 30.667 mu L/L, and the 95% confidence interval is 27.3280-33.7432 mu L/L.
TABLE 1 determination of the bacteriostatic effect of carvacrol on tobacco ralstonia solanacearum
2.2 carvacrol inhibits the activity of tobacco black shank bacteria
As shown in table 2, carvacrol has a good bacteriostatic effect on phytophthora parasitica, and the toxicity equation is that the regression equation of the toxicity is y (8.8829 x) -8.0402(R2 (0.9862)), and Ec50 (29.38 μ L/L).
TABLE 2 measurement results of the carvacrol inhibition of tobacco black shank
Example 3
The carvacrol-containing composition has bacteriostatic activity on tobacco bacterial wilt pathogenic bacteria (Ralstonia solanacearum).
Materials and methods
1.1 test strains
Tobacco bacterial wilt pathogen (Ralstonia solanacearum).
1.2 the composition of the invention inhibits the activity of ralstonia solanacearum
Mixing carvacrol and wild chrysanthemum essential oil according to the volume ratio of 1-10: 10-1, and measuring the bacterial inhibition activity of different combinations on ralstonia solanacearum on a 96-hole cell culture plate. Washing ralstonia solanacearum activated for 12h on NA slant culture medium with sterile water, and diluting to bacteria content of 108CFU/mL, respectively adding 190 μ L of ralstonia solanacearum into 10 μ L culture wells containing carvacrol and chrysanthemum indicum essential oil compounds with different concentrations, performing shaking culture at constant temperature of 30 ℃ for 24h at 150r/min, and measuring the absorbance value of the bacterial liquid at 600 nm. Each treatment was repeated 3 times, using the inoculum solution without the drug as a control. Calculation of EC50And (4) calculating the co-toxicity coefficient of the two medicaments by adopting a Sun Yunpei method, and determining the synergistic ratio.
2. Results and analysis
2.1 the composition of the invention inhibits the activity of Ralstonia solanacearum
The results in table 3 show that the volume ratio of carvacrol to chrysanthemum indicum essential oil is 1-10: 10-1, the synergistic effect is obvious, and the highest co-toxicity coefficient is 199.79, especially when the volume ratio is 9: 1-7: 3.
TABLE 3 combination virulence assay results for carvacrol and wild chrysanthemum essential oil composition against ralstonia solanacearum
| Medicament | EC50 | TTI | ATI | CTC |
| Carvacrol (A) | 29.38 | 100.00 | ||
| Wild chrysanthemum essential oil (B) | 190.265 | 620.42 | ||
| A:B9:1 | 24.517 | 152.04 | 79.95 | 190.18 |
| A:B8:2 | 31.326 | 204.08 | 102.15 | 199.79 |
| A:B7:3 | 46.884 | 256.13 | 152.88 | 167.53 |
| A:B6:4 | 59.297 | 308.17 | 193.36 | 159.38 |
| A:B5:5 | 72.314 | 360.21 | 235.80 | 152.76 |
| A:B4:6 | 79.811 | 412.25 | 260.25 | 158.41 |
| A:B3:7 | 95.243 | 464.30 | 310.57 | 149.50 |
| A:B2:8 | 119.114 | 516.34 | 388.41 | 132.94 |
| A:B1:9 | 123.285 | 568.38 | 402.01 | 141.38 |
Example 4
The carvacrol-containing composition of the invention is effective against Phytophthora nicotianae (Phytophthora nicotiana).
Materials and methods
1.1 test strains
Tobacco Phytophthora nicotianae (Phytophthora nicotianae).
1.2 the inventive compositions inhibit the activity of the bacterium melanogaster
A hypha growth rate method is adopted. Mixing carvacrol and wild chrysanthemum essential oil according to the volume ratio of 1-10: 10-1, preparing conventional oat culture media (at about45 ℃) plates with different concentrations by using DMSO (0.5%, v/v), inoculating phytophthora nicotianae cakes (5mm) to the conventional oat culture media with different concentrations, culturing for 5 days at 28 ℃, taking DMSO with the same dilution ratio as a control, measuring the colony diameter by a cross method, and calculating the hypha growth inhibition rate and EC50The value is obtained. Calculation of EC50And (4) calculating the co-toxicity coefficient of the two medicaments by adopting a Sun Yunpei method, and determining the synergistic ratio.
2. Results and analysis
2.1 compounding carvacrol and wild chrysanthemum essential oil to inhibit activity of phytophthora parasitica
The results in table 4 show that the volume ratio of carvacrol to chrysanthemum indicum essential oil is 1-10: 10-1, the synergistic effect is obvious, and the highest co-toxicity coefficient is 199.79, especially when the volume ratio is 9: 1-7: 3.
TABLE 4 combination virulence assay results for carvacrol and wild chrysanthemum essential oil compositions against Nicotiana tabacum
| Medicament | EC50 | TTI | ATI | CTC |
| Carvacrol (A) | 29.38 | 100.00 | ||
| Wild chrysanthemum essential oil (B) | 48.4531 | 158.00 | ||
| A:B9:1 | 20.662 | 106.49 | 70.33 | 151.42 |
| A:B8:2 | 21.537 | 112.98 | 73.30 | 154.13 |
| A:B7:3 | 22.056 | 119.48 | 75.07 | 159.15 |
| A:B6:4 | 26.329 | 125.97 | 89.62 | 140.56 |
| A:B5:5 | 28.913 | 132.46 | 98.41 | 134.60 |
| A:B4:6 | 30.824 | 138.95 | 104.91 | 132.44 |
| A:B3:7 | 32.731 | 145.44 | 111.41 | 130.55 |
| A:B2:8 | 34.579 | 151.93 | 117.70 | 129.09 |
| A:B1:9 | 35.057 | 158.43 | 119.32 | 132.77 |
Example 5
The fungicide prepared in example 1 was subjected to a pharmacodynamic experiment for controlling tobacco bacterial wilt in a tobacco field of Nanping, Fujian province, a control agent was 20% Thiodiazole copper suspending agent, and the test results are shown in Table 5. The test agents are diluted by 500 times and irrigated into roots, and each plant is 100 mL. The test was set up in triplicate, three rows of zones, 15 cigarettes per row, 45 cigarettes per cell. The application is carried out for 1 time in the tobacco agglomeration period (1 month after transplanting), and the application is carried out for 2 times continuously at intervals of 10 days, and the application is carried out for 3 times in total.
As shown in Table 5, the bactericide in example 1 has a certain control effect on tobacco bacterial wilt, and the control effect is more than 50% 10 days after application, which is equivalent to the control effect of a control medicament of 20% of thiabendazole copper suspending agent.
TABLE 5 field test results of efficacy of fungicide against tobacco bacterial wilt in example 1 (Fujian Nanping)
| Medicament | Dosage (ml/mu) | Average controlling effect (%) |
| Carvacrol | 100 | 51.3 |
| Example 1 | 100 | 55.7 |
| 20% Thiodiazole copper suspension | 100 | 50.8 |
Example 6
The fungicide prepared in example 2 was subjected to a pharmacodynamic experiment for controlling tobacco bacterial wilt in a tobacco field of Nanping, Fujian province, a control agent was 20% of thiediazole copper suspending agent, and the test results are shown in Table 6. The test agents are diluted by 500 times and irrigated into roots, and each plant is 100 mL. The test was set up in triplicate, three rows of zones, 15 cigarettes per row, 45 cigarettes per cell. The application is carried out for 1 time in the tobacco agglomeration period (1 month after transplanting), and the application is carried out for 2 times continuously at intervals of 10 days, and the application is carried out for 3 times in total.
As shown in Table 6, the bactericide in example 2 has a certain control effect on tobacco bacterial wilt, and the control effect is more than 50% 10 days after application, which is equivalent to the control effect of a control medicament of 20% of thiabendazole copper suspending agent.
TABLE 6 field test results of efficacy of fungicide against tobacco bacterial wilt in example 2 (Fujian Nanping)
| Medicament | Dosage (ml/mu) | Average controlling effect (%) |
| Carvacrol | 100 | 51.3 |
| Example 2 | 100 | 53.2 |
| 20% Thiodiazole copper suspension | 100 | 50.8 |
Example 7
Uniformly mixing 1kg of soil and 2-3 g of phytophthora parasitica strain grains per pot, selecting tobacco seedlings with consistent growth, transplanting, adding 50ml of prepared diluent (500-time diluent, diluted and dissolved by 5% DMSO) of the bactericide in the embodiment 1 into each pot, and performing root irrigation treatment for 3 pots for each treatment. 58% metalaxyl manganese zinc was used as a control. Every 10 th day after inoculation, the incidence of black shank of each treatment is investigated by taking plants as units after noon in sunny days, and the disease index and the relative prevention and treatment effect are calculated.
As shown in table 7, the fungicide of example 1 has a good control effect on tobacco black shank, and the control effect reaches more than 70% 10 days after application, which is similar to that of a control medicament 58% metalaxyl manganese zinc.
TABLE 7 example 1 potted plant test results of fungicide control of tobacco black shank
| Medicament | Dosage of | Average controlling effect (%) |
| Carvacrol | 500 times liquid | 72.3 |
| Example 1 | 500 times liquid | 78.1 |
| 58% metalaxyl manganese zinc wettable powder | 500 times liquid | 82.5 |
Example 8
Uniformly mixing 1kg of soil and 2-3 g of phytophthora parasitica strain grains per pot, selecting tobacco seedlings with consistent growth, transplanting, adding 50ml of prepared diluent (500-time diluent, diluted and dissolved by 5% DMSO) of the bactericide in the embodiment 2 into each pot, and performing root irrigation treatment for 3 pots of soil for each treatment. 58% metalaxyl manganese zinc was used as a control. Every 10 th day after inoculation, the incidence of black shank of each treatment is investigated by taking plants as units after noon in sunny days, and the disease index and the relative prevention and treatment effect are calculated.
As shown in table 8, the fungicide of example 2 has a good control effect on tobacco black shank, and the control effect reaches more than 70% 10 days after application, which is similar to that of a control medicament 58% metalaxyl manganese zinc.
TABLE 8 example 2 potted plant test results of fungicide control of tobacco black shank
| Medicament | Dosage of | Average controlling effect (%) |
| Carvacrol | 500 times liquid | 72.3 |
| Example 2 | 500 times liquid | 73.9 |
| 58% metalaxyl manganese zinc wettable powder | 500 times liquid | 82.5 |
Example 9
The test was carried out in tobacco black shank disease nursery of tobacco institute of agriculture academy of sciences of china, and china, province, Shandong province. The tobacco variety was a susceptible variety, little gold 1025, with a total of 6 treatments (table 7) set for the trial, with 3 replicates per treatment. Each test cell had 45 cigarettes, 3 rows of zones, 15 cigarettes per row, and random block arrangements. The pesticide is applied for 1 time during transplanting, and is applied for 1 time every 7-10 days when diseased plants appear in the field, and is continuously applied for 2 times. The application method comprises irrigating the root of stem with 100mL of medicinal liquid per plant. Disease index was investigated 10 days after the last application, disease grade was investigated in units of plants, grade 0: the whole plant is disease-free; level 1: the stem lesion does not exceed 1/3 of the stem circumference, or the leaf below 1/3 withers; and 3, level: the stem scabs surround the stem circumference 1/3-1/2, or 1/3-1/2 leaves are slightly withered, or a few lower leaves have scabs; and 5, stage: stem lesions exceed 1/2 of the stem girth but do not completely encircle the stem girth, or 1/2-2/3 leaves wither; and 7, stage: the stem lesions all encircle the stem circumference, or the leaves above 2/3 wither; and 9, stage: the diseased plants die basically.
Disease index is 100 × Σ (number of diseased plants at each stage × representative value at each stage)/(total number of investigated plants × 9); relative prevention and treatment effect (%) (control disease index-treatment disease index)/control disease index × 100.
The results are shown in table 9, the bactericide of example 1 has a good control effect on tobacco black shank, and the control effect reaches more than 65% 10 days after the last application, which is equivalent to 58% of metalaxyl manganese zinc of a control medicament.
Table 9 results of field test for controlling tobacco black shank with the fungicide of example 1 (Qingdao instant ink)
| Medicament | Dosage of | Average controlling effect (%) |
| Carvacrol | 500 times liquid | 65.5 |
| Example 1 | 500 times liquid | 68.3 |
| 58% metalaxyl manganese zinc wettable powder | 500 times liquid | 70.7 |
Example 10
The test was carried out in tobacco black shank disease nursery of tobacco institute of agriculture academy of sciences of china, and china, province, Shandong province. The tobacco variety was a susceptible variety, little gold 1025, with a total of 6 treatments (table 7) set for the trial, with 3 replicates per treatment. Each test cell had 45 cigarettes, 3 rows of zones, 15 cigarettes per row, and random block arrangements. The pesticide is applied for 1 time during transplanting, and is applied for 1 time every 7-10 days when diseased plants appear in the field, and is continuously applied for 2 times. The application method comprises irrigating the root of stem with 100mL of medicinal liquid per plant. Disease index was investigated 10 days after the last application, disease grade was investigated in units of plants, grade 0: the whole plant is disease-free; level 1: the stem lesion does not exceed 1/3 of the stem circumference, or the leaf below 1/3 withers; and 3, level: the stem scabs surround the stem circumference 1/3-1/2, or 1/3-1/2 leaves are slightly withered, or a few lower leaves have scabs; and 5, stage: stem lesions exceed 1/2 of the stem girth but do not completely encircle the stem girth, or 1/2-2/3 leaves wither; and 7, stage: the stem lesions all encircle the stem circumference, or the leaves above 2/3 wither; and 9, stage: the diseased plants die basically.
Disease index is 100 × Σ (number of diseased plants at each stage × representative value at each stage)/(total number of investigated plants × 9); relative prevention and treatment effect (%) (control disease index-treatment disease index)/control disease index × 100.
The results are shown in table 10, the bactericide of example 2 has a good control effect on tobacco black shank, and the control effect reaches more than 65% 10 days after the last application, and is equivalent to 58% of metalaxyl manganese zinc serving as a control medicament.
TABLE 10 example 2 Fungicide field test results for control of tobacco Black shank (Qingdao instant ink)
| Medicament | Dosage of | Average controlling effect (%) |
| Carvacrol | 500 times liquid | 65.5 |
| Example 2 | 500 times liquid | 66.4 |
| 58% metalaxyl manganese zinc wettable powder | 500 times liquid | 70.7 |
The embodiments show that the plant source composition provided by the invention has good control effects on tobacco bacterial wilt and black shank. The bactericide prepared by the composition has the control effect on tobacco bacterial wilt of more than 50 percent and the control effect on tobacco black shank of more than 65 percent.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (12)
1. The sterilizing composition containing carvacrol and chrysanthemum indicum essential oil is characterized by comprising carvacrol and chrysanthemum indicum essential oil, wherein the volume ratio of carvacrol to chrysanthemum indicum essential oil is (9-1) to (1-9).
2. The bactericidal composition of claim 1, wherein the volume ratio of the carvacrol to the wild chrysanthemum essential oil is (7-9): (3-2).
3. The bactericide is characterized by comprising the following raw materials in parts by mass: 15-25 parts of the bactericidal composition as set forth in claim 1 or 2, 3.5-6.5 parts of an emulsifier, 1.5-2.5 parts of a co-emulsifier, 2-4 parts of an antifreeze, 0.5-1.5 parts of an antioxidant and 51-87 parts of water.
4. The bactericide according to claim 3, wherein the emulsifier comprises one or more of polyether phosphate, phenol ether phosphate, alkylphenol ethoxylate and calcium dodecylbenzenesulfonate.
5. The fungicide according to claim 4, wherein said phenol ether phosphate ester comprises one or both of phenethylphenol polyoxyethylene ether phosphate ester and nonylphenol polyoxyethylene ether phosphate ester.
6. The fungicide according to claim 3, wherein said co-emulsifier comprises one or several of n-butanol, n-hexanol and tridecanol.
7. The bactericide according to claim 3, wherein the antifreezing agent comprises ethylene glycol and/or glycerin.
8. The biocide of claim 3, wherein said antioxidant comprises dibutylhydroxytoluene.
9. The use of the bactericidal composition according to claim 1 or 2 for controlling bacterial wilt diseases, wherein the volume ratio of carvacrol to chrysanthemum indicum essential oil is 9: 1.
10. The use of the fungicidal composition of claim 1 or 2 for the control of blackleg disease.
11. The use of the fungicide according to any one of claims 3 to 8 for controlling bacterial wilt diseases; the volume ratio of the carvacrol to the wild chrysanthemum essential oil is 9: 1.
12. The use of a fungicide according to any one of claims 3 to 8 for the control of phytophthora parasitica.
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