CN110146615A - 一种同时测血清中尼古丁、3-(吡咯烷-2-基)吡啶、吡啶吡咯酮以及睾酮含量的方法 - Google Patents
一种同时测血清中尼古丁、3-(吡咯烷-2-基)吡啶、吡啶吡咯酮以及睾酮含量的方法 Download PDFInfo
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- CN110146615A CN110146615A CN201910482380.XA CN201910482380A CN110146615A CN 110146615 A CN110146615 A CN 110146615A CN 201910482380 A CN201910482380 A CN 201910482380A CN 110146615 A CN110146615 A CN 110146615A
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- pyridine
- nicotine
- pyrrolidin
- ketone
- serum
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- 238000000034 method Methods 0.000 title claims abstract description 68
- MYKUKUCHPMASKF-UHFFFAOYSA-N nornicotine Chemical compound C1CCNC1C1=CC=CN=C1 MYKUKUCHPMASKF-UHFFFAOYSA-N 0.000 title claims abstract description 58
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 title claims abstract description 38
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 title claims abstract description 38
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- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 54
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- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 6
- 239000012086 standard solution Substances 0.000 claims description 6
- -1 pyrrole Pyridine pyrrolones Chemical class 0.000 claims description 5
- 239000000758 substrate Substances 0.000 claims description 4
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 3
- 238000010828 elution Methods 0.000 claims description 3
- 235000019253 formic acid Nutrition 0.000 claims description 3
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- 206010011224 Cough Diseases 0.000 claims description 2
- 230000005526 G1 to G0 transition Effects 0.000 claims 1
- RDRCCJPEJDWSRJ-UHFFFAOYSA-N pyridine;1h-pyrrole Chemical compound C=1C=CNC=1.C1=CC=NC=C1 RDRCCJPEJDWSRJ-UHFFFAOYSA-N 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 25
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- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 6
- KAESVJOAVNADME-UHFFFAOYSA-N Pyrrole Chemical compound C=1C=CNC=1 KAESVJOAVNADME-UHFFFAOYSA-N 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 230000000391 smoking effect Effects 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 4
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- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 3
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 2
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- AQCRXZYYMOXFAN-UHFFFAOYSA-N 2-(1-methyl-2-pyrrolidinyl)-pyridine Chemical compound CN1CCCC1C1=CC=CC=N1 AQCRXZYYMOXFAN-UHFFFAOYSA-N 0.000 description 1
- XVMSFILGAMDHEY-UHFFFAOYSA-N 6-(4-aminophenyl)sulfonylpyridin-3-amine Chemical compound C1=CC(N)=CC=C1S(=O)(=O)C1=CC=C(N)C=N1 XVMSFILGAMDHEY-UHFFFAOYSA-N 0.000 description 1
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- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
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CN201910482380.XA CN110146615B (zh) | 2019-06-04 | 2019-06-04 | 一种同时测血清中尼古丁、3-(吡咯烷-2-基)吡啶、吡啶吡咯酮以及睾酮含量的方法 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN116622792A (zh) * | 2022-10-19 | 2023-08-22 | 修实生物医药(南通)有限公司 | 一种使用酶催化合成(s)-烟碱的方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102590386A (zh) * | 2012-02-16 | 2012-07-18 | 广西中烟工业有限责任公司 | 一种吸烟者尿液样品中烟碱及其代谢物的检测方法 |
CN103776928A (zh) * | 2014-01-13 | 2014-05-07 | 红云红河烟草(集团)有限责任公司 | 一种检测尿液中3-羟基可替宁的方法 |
CN104020241A (zh) * | 2014-06-11 | 2014-09-03 | 中国烟草总公司郑州烟草研究院 | 动物血、脑样品中痕量烟碱及其主要代谢物的同步分析方法 |
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- 2019-06-04 CN CN201910482380.XA patent/CN110146615B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102590386A (zh) * | 2012-02-16 | 2012-07-18 | 广西中烟工业有限责任公司 | 一种吸烟者尿液样品中烟碱及其代谢物的检测方法 |
CN103776928A (zh) * | 2014-01-13 | 2014-05-07 | 红云红河烟草(集团)有限责任公司 | 一种检测尿液中3-羟基可替宁的方法 |
CN104020241A (zh) * | 2014-06-11 | 2014-09-03 | 中国烟草总公司郑州烟草研究院 | 动物血、脑样品中痕量烟碱及其主要代谢物的同步分析方法 |
Non-Patent Citations (4)
Title |
---|
P. SEEMA等: "Protective Effect of Selenium on Nicotine-Induced Testicular Toxicity in Rats", 《BIOL TRACE ELEM RES》 * |
TERESA R. GRAY等: "Quantification of nicotine, cotinine, trans-3 -hydroxycotinine, nornicotine and norcotinine in human meconium by liquid chromatography/tandem mass spectrometry", 《JOURNAL OF CHROMATOGRAPHY B》 * |
周宛虹等: "LC-MS/MS 法快速高效检测尿液中尼古丁及其 9 种代谢物", 《化学学报》 * |
毛健 等: "微透析-UPLC-MS/MS法同时测定活体大鼠脑内烟碱及其代谢物", 《烟草科技》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116622792A (zh) * | 2022-10-19 | 2023-08-22 | 修实生物医药(南通)有限公司 | 一种使用酶催化合成(s)-烟碱的方法 |
CN116622792B (zh) * | 2022-10-19 | 2024-03-08 | 修实生物医药(南通)有限公司 | 一种使用酶催化合成(s)-烟碱的方法 |
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Inventor after: Zhang Weiyi Inventor after: He Ruyi Inventor after: Gao Qingying Inventor after: Chen Jingjing Inventor after: Zheng Minghua Inventor after: Liang Xiumei Inventor after: Xu Jing Inventor after: Xu Renai Inventor after: Zhang Jing Inventor after: Xie Shibing Inventor after: Zhang Hui Inventor after: Lin Dingpeng Inventor before: Zhang Weiyi Inventor before: He Ruyi Inventor before: Gao Qingying Inventor before: Chen Jingjing Inventor before: Zheng Minghua Inventor before: Xu Jing Inventor before: Xu Renai Inventor before: Liang Xiumei Inventor before: Zhang Jing Inventor before: Xie Shibing Inventor before: Zhang Hui Inventor before: Lin Dingpeng |
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