CN110122426A - A method of trapping coccinella septempunctata oviposition - Google Patents
A method of trapping coccinella septempunctata oviposition Download PDFInfo
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- CN110122426A CN110122426A CN201910403938.0A CN201910403938A CN110122426A CN 110122426 A CN110122426 A CN 110122426A CN 201910403938 A CN201910403938 A CN 201910403938A CN 110122426 A CN110122426 A CN 110122426A
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- oviposition
- coccinella septempunctata
- trapping
- trap
- pea
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- 230000017448 oviposition Effects 0.000 title claims abstract description 95
- 241000131068 Coccinella septempunctata Species 0.000 title claims abstract description 57
- 238000000034 method Methods 0.000 title claims abstract description 33
- 241000219843 Pisum Species 0.000 claims abstract description 33
- 241001124076 Aphididae Species 0.000 claims abstract description 20
- 239000000463 material Substances 0.000 claims abstract description 17
- 241000238631 Hexapoda Species 0.000 claims abstract description 8
- 241000196324 Embryophyta Species 0.000 claims abstract description 6
- 241000255749 Coccinellidae Species 0.000 claims description 11
- 229920000742 Cotton Polymers 0.000 claims description 11
- 239000003755 preservative agent Substances 0.000 claims description 5
- 230000002335 preservative effect Effects 0.000 claims description 5
- 230000001419 dependent effect Effects 0.000 claims description 4
- 238000007654 immersion Methods 0.000 claims description 4
- 210000005239 tubule Anatomy 0.000 claims description 3
- 235000013601 eggs Nutrition 0.000 abstract description 8
- 238000011084 recovery Methods 0.000 abstract description 5
- 102000002322 Egg Proteins Human genes 0.000 abstract description 4
- 108010000912 Egg Proteins Proteins 0.000 abstract description 4
- 210000004681 ovum Anatomy 0.000 abstract description 4
- 230000002776 aggregation Effects 0.000 abstract description 2
- 238000004220 aggregation Methods 0.000 abstract description 2
- 230000029305 taxis Effects 0.000 abstract description 2
- 241000872198 Serjania polyphylla Species 0.000 abstract 1
- 239000000843 powder Substances 0.000 description 14
- 244000269722 Thea sinensis Species 0.000 description 12
- 239000011159 matrix material Substances 0.000 description 12
- 229920002472 Starch Polymers 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- 235000019698 starch Nutrition 0.000 description 10
- 239000008107 starch Substances 0.000 description 10
- 235000013616 tea Nutrition 0.000 description 10
- 238000005192 partition Methods 0.000 description 8
- 235000013339 cereals Nutrition 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 229920001817 Agar Polymers 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 230000003020 moisturizing effect Effects 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241001600407 Aphis <genus> Species 0.000 description 2
- 229920002261 Corn starch Polymers 0.000 description 2
- 239000008120 corn starch Substances 0.000 description 2
- 229940099112 cornstarch Drugs 0.000 description 2
- 235000009569 green tea Nutrition 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 239000012780 transparent material Substances 0.000 description 2
- 235000020334 white tea Nutrition 0.000 description 2
- 241001425390 Aphis fabae Species 0.000 description 1
- 241000254173 Coleoptera Species 0.000 description 1
- 241000223924 Eimeria Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 235000005072 Vigna sesquipedalis Nutrition 0.000 description 1
- 244000090207 Vigna sesquipedalis Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000000443 biocontrol Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000021278 navy bean Nutrition 0.000 description 1
- 239000010899 old newspaper Substances 0.000 description 1
- 244000062645 predators Species 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention belongs to insect Cultivating techniques fields, and in particular to a method of trap coccinella septempunctata oviposition.The method of the present invention for trapping coccinella septempunctata oviposition, there is the pea seedlings of sufficient pea tail reparing aphid as trap material to plant, using coccinella septempunctata to the taxis of aphid, coccinella septempunctata is placed in oviposition container with the pea seedlings for being loaded with sufficient pea tail reparing aphid and carries out trapping oviposition, effectiveness is trapped with apparent, coccinella septempunctata can be trapped together, be conducive to its aggregation oviposition；And with high smooth PE bags of black with a thickness of 0.1-0.2mm of quantity of laying eggs be oviposition carrier, effectively increase the spawning rate and the rate of recovery of coccinella septempunctata, it efficiently solves the problems, such as that coccinella septempunctata egg laying amount and the ovum rate of recovery be not high, guarantees that its population colonization plays a significant role for it.
Description
Technical field
The invention belongs to insect Cultivating techniques fields, and in particular to a method of trap coccinella septempunctata oviposition.
Background technique
Aphid is a kind of agriculturally very harmful pest, all brings irreversible damage to plant and environment, no matter
Greenhouse all once caused no small disaster still in crop field.Currently, to the prevention and treatment of aphid mainly based on pesticide control, although
Significant effect but inevitably cause due to chemical pesticide dosage is excessively high and bring great chemical drug pollution, cause
Secondary hazards.
Coccinella septempunctata (Coccinella septempunctata L.) is one of Dominant Natural Enemies insect of aphid, seven-star wooden dipper
Eimeria is on the chief crops such as wheat, cotton, oil plant and vegetables in coleoptera Coleoptera, Coccinellidae Coccinellidae
The important predator of aphid.Research both at home and abroad is it was demonstrated that being eliminated aphis using coccinella septempunctata is that a kind of fabulous biology is anti-
Control mode.Application chemical pesticide can be effectively reduced with the bio-control method that worm controls worm to pollute to environment bring and to personal strong
The harm of health bring, has important ecological significance.With the raising of biological control commercial applications degree, about predatism wooden dipper
The research of worm growth and development, population large-scale breeding and application security etc. has become new research hotspot.
In the indoors artificial method for breeding of existing coccinella septempunctata, mature system method can not enable coccinella septempunctata steady
Fixed, the good character of holding successive propagation gets off.Be constantly present in current method coccinella septempunctata can complete the history of life,
But the problem of fecundity is not strong, lacks the research to egg laying amount method, can not achieve a large amount of continuously rearings.The test worm of coccinella septempunctata
It is acquired by field, without the stable worm sources of artificial continuous multi-generation raising, limits the research work to it, also limit it
Release application is carried out in field as natural enemy insect.
Early-stage study shows that it is a kind of relatively good breeding seven-star that black bean aphid raising coccinella septempunctata is cultivated using indoors artificial
The method of ladybug.Wherein, the factors such as environment photoperiod, varying environment color have the spawning habit and egg laying amount of coccinella septempunctata
It significantly affects.And the method that coccinella septempunctata is effective, concentrates oviposition can be trapped, the extensive artificial propagation for coccinella septempunctata
It plays an important role, provides premise for Field information coccinella septempunctata.
Summary of the invention
For this purpose, technical problem to be solved by the present invention lies in provide a kind of method for trapping coccinella septempunctata oviposition.
In order to solve the above technical problems, a kind of method for trapping coccinella septempunctata oviposition of the present invention, that is, include that will lure
Acquisition means, which are placed in coccinella septempunctata dependent insect cage, carries out the step of trapping oviposition；
Described to trap device, including open oviposition container, the oviposition container is internally provided with the partition with aperture, makes
The oviposition container is separated into upper layer and traps space and lower layer and lays eggs space；
The upper layer traps and is provided with funnel-form trap in space, and the open end of the trap, which is provided with to contain, traps material
The lure of material, the tubule end of the trap pass through the aperture and protrude into the oviposition space；
Lower layer's oviposition is equipped with oviposition carrier in space.
The trap material is the pea seedlings for being loaded with pea tail reparing aphid.
The root of the pea seedlings is provided with immersion absorbent cotton.
The external sheath of the immersion absorbent cotton has preservative film.
The quantity of the pea tail reparing aphid is 180-220 heads/plant of pea seedlings.
The oviposition carrier is PE bags of dark color.
The oviposition carrier is black PE bags.
The oviposition carrier is that black is PE bags smooth.
It is described oviposition carrier with a thickness of 0.1-0.2mm.
The injected volume of the pea tail reparing aphid is 500-600:1 with the quantity ratio wait trap oviposition ladybug.
In order to further increase the gathering effect to coccinella septempunctata oviposition, lure of the present invention containing described in addition to trapping
Outside material, it is additionally provided with and traps matrix；
Specifically, the matrix that traps includes tea powder, cereal starch, water and agar, the tea powder, cereal starch, water and
The mass ratio of agar is 10-15:8-12:100:2.
Preferably, the mass ratio of the tea powder, cereal starch and water is 12:10:100:2.
Specifically, the tea powder includes green tea powder, white tea powder and/or olong tea powder.
More preferably, the tea powder includes green tea powder, white tea powder and the olong tea powder that mass ratio is 2:1:1.
Specifically, the cereal starch includes cornstarch and/or wheaten starch.
More preferably, the cereal starch includes the cornstarch and wheaten starch that mass ratio is 1:1.
The method for trapping matrix is prepared the invention also discloses a kind of, that is, includes taking a selected amount of tea powder, paddy
The step of object starch, water and agar are mixed.
The method of the present invention for trapping coccinella septempunctata oviposition is to trap material to be loaded with the pea seedlings of sufficient pea tail reparing aphid
Material places it in oviposition container using coccinella septempunctata to the taxis of aphid and carries out trapping oviposition, have and significantly trap effect
With, coccinella septempunctata can be trapped together, be conducive to its aggregation oviposition；And with high smooth with a thickness of 0.1- of quantity of laying eggs
PE bags of black of 0.2mm are oviposition carrier, effectively increase the spawning rate and the rate of recovery of coccinella septempunctata, efficiently solve seven-star wooden dipper
Worm egg laying amount and the not high problem of the ovum rate of recovery, guarantee it its population colonization plays a significant role.
The lure material of the present invention for trapping device, it is contemplated that number of the growth conditions of pea seedlings for pea tail reparing aphid
Amount and survival rate have great influence, and pea tail reparing aphid quantity influences the congregational rate and egg laying amount of ladybug, in view of wet
Spending has decisive role to the fresh of holding pea seedlings, therefore is soaked in the pea seedlings of trap material with absorbent cotton in the present invention
The method for surrounding root afterwards, being then coated with one layer of preservative film again prevents moisture on absorbent cotton from evaporating, to guarantee the health of pea seedlings
Growth, to guarantee that aphis population is stablized on pea seedlings.
In order to improve the gathering effect of described device, it further joined to help to improve in lure of the present invention and trap
Effect traps matrix, and the matrix that traps adds water and agar that solid state substrate is made using selected tea powder, cereal starch as raw material
Shape effectively increases the gathering effect of coccinella septempunctata using the smell effect of Adult Coccinella Septempunctata.
The method of the present invention for trapping coccinella septempunctata oviposition combines become chromatic effect and the smell effect of Adult Coccinella Septempunctata
It should propose and new trap oviposition method, it is ensured that all adults can be laid eggs by concentrating, and lay eggs mediator and adult trap
Device is used cooperatively, and reduces ladybug and ovum is produced in other positions, and the especially hidden, position that is not easily recycled is improved into
It is scarce with taking a lot of work, cannot reuse to efficiently solve currently used coccinella septempunctata oviposition method for the rate of recovery of worm oviposition
Point, to realize that the factorial production ladybug provides a new approach.
Detailed description of the invention
In order to make the content of the present invention more clearly understood, it below according to specific embodiments of the present invention and combines
Attached drawing, the present invention is described in further detail, wherein
Fig. 1 is the schematic perspective view of the device of the present invention for trapping coccinella septempunctata oviposition；
Fig. 2 is the top view of the device of the present invention for trapping coccinella septempunctata oviposition；
Fig. 3 is the A-A sectional view in Fig. 2；
Fig. 4 is the stereochemical structure perspective view of the device of the present invention for trapping coccinella septempunctata oviposition；
Fig. 5 is the main view of the device of the present invention for trapping coccinella septempunctata oviposition；
Appended drawing reference indicates in figure are as follows: 1- oviposition container, 2- partition, 3- aperture, 4- trap, 5- lure, 6- trap sky
Between, 7- oviposition space, 8- oviposition carrier.
Specific embodiment
Structure as shown in Figure 1, the device of the present invention for trapping coccinella septempunctata oviposition, produces including open transparent material
Ovum container 1, the oviposition container 1 is internally provided with the partition 2 with aperture 3, so that the oviposition container 1 is separated into upper layer and lures
Collect space 6 and lower layer's oviposition space 7；The partition 2 is preferably transparent material partition, and the partition 2 is preferably provided at institute
The place by the upper position of oviposition container 1 is stated, laying eggs for lower layer, there are enough spaces in space 7.The aperture 3 of the partition 2 is preferred
It is set to the center position of the partition 2.
The upper layer traps and is provided with funnel-form trap 4 in space 6, and the open end of the trap 4 is provided with to contain and lure
Collect the lure 5 of material, the tubule end of the trap 4 passes through the aperture 3 and protrudes into the oviposition space；The aperture 3
Aperture is advisable with the thin tube end diameter for being suitable for the trap 4.The lure 5 can pass through bracket or hook, iron wire etc.
The funnel-form that mode is fixed on the trap 4 is hollow interior, and in structure as shown in Figure 1, the lure 5 is to be tied up by iron wire
Tight mode, be fixed in trap 4 funnel-form it is hollow in, it is empty that effective guarantee coccinella septempunctata enters lower layer oviposition
Between 7 space.
The trap material is preferably the fresh pea seedlings for being loaded with pea tail reparing aphid, chooses the growing way for growing to 10cm or so
Preferable fresh pea seedlings proportionally (every plant of pea seedlings about 200) is chosen aphid to uprooping on pea seedlings with writing brush
Afterwards, it is soaked with absorbent cotton and surrounds pea seedlings root, and cover preservative film moisturizing on absorbent cotton, trap material is made.The pea
Beans repair the injected volume of tail aphid and the quantity ratio wait trap oviposition ladybug is 500-600:1.
In order to further increase the gathering effect to coccinella septempunctata oviposition, lure of the present invention containing described in addition to trapping
Outside material, it is additionally provided with and traps matrix；Specifically, described trap matrix to be the selected of 10-15:8-12:100:2 by mass ratio
Gel state solid matrix made from the tea powder of type, cereal starch, water and agar, it is described trap perforation is opened up in matrix after will
After the root of the pea seedlings passes through the perforation of the solid matrix, then is soaked with absorbent cotton and surround pea seedlings root, and de-
Preservative film moisturizing is covered on rouge cotton, and trap material is made.
Of the present invention to trap in oviposition device, lower layer's oviposition is equipped with oviposition carrier 8 in space 7.The oviposition
Carrier 8 is preferably PE bags of dark color, and more preferably black is PE bags smooth, and preferably laying depth is 0.1-0.2mm.
Embodiment 1-6
In the following embodiments of the present invention, different trap materials are selected respectively and trap the lure (see the table below 1) of matrix, utilize
It is shown in FIG. 1 trap device to coccinella septempunctata carry out oviposition trap.The device that traps is placed in equipped with 50 pairs of 7d after sprouting wings
It in the dependent insect cage of Adult Coccinella Septempunctata, is flushed with ground, and cleans up the food of ladybug.
After 24 hours, the oviposition carrier for being placed in dependent insect cage bottom is taken out, the PE bag for adhering to pieces of an egg is cut with scissors, is placed in
In culture dish with filter paper, add water moisturizing, culture hatching is carried out under the conditions of 25 DEG C, relative humidity 60-80%, statistics oviposition carries
The quantity of Ladybird egg on body records statistical result in the following table 1.
1 embodiment ladybug of table oviposition quantity
Comparative example 1
The structure that oviposition device is trapped described in this comparative example is same as Example 2, and difference is only that the oviposition carries
Body is black print paper.After measured, egg laying amount is 378.
Comparative example 2
The structure that oviposition device is trapped described in this comparative example is same as Example 2, and difference is only that the oviposition carries
Body is old newspaper.After measured, egg laying amount is 332.
From upper table data it is found that the device of the present invention for trapping coccinella septempunctata oviposition, can effectively trap seven-star wooden dipper
Worm oviposition, especially traps the setting of matrix, more effectively improves the egg laying amount of coccinella septempunctata.
Obviously, the above embodiments are merely examples for clarifying the description, and does not limit the embodiments.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or
It changes.There is no necessity and possibility to exhaust all the enbodiments.And it is extended from this it is obvious variation or
It changes still within the protection scope of the invention.
Claims (10)
1. a kind of method for trapping coccinella septempunctata oviposition, which is characterized in that be placed in coccinella septempunctata dependent insect cage including device will be trapped
In carry out the step of trapping oviposition；
The device that traps includes open oviposition container (1), oviposition container (1) be internally provided with aperture (3) every
Plate (2), so that the oviposition container (1) is separated into upper layer and traps space (6) and lower layer's oviposition space (7)；
The upper layer traps to be provided with funnel-form trap (4) in space (6), and the open end of the trap (4), which is provided with, to be contained
The lure (5) of trap material, the tubule end of the trap (4) pass through the aperture (3) and protrude into the oviposition space；
Oviposition carrier (8) is equipped in lower layer's oviposition space (7).
2. the method according to claim 1 for trapping coccinella septempunctata oviposition, which is characterized in that the trap material is to be loaded with
The pea seedlings of pea tail reparing aphid.
3. the method according to claim 2 for trapping coccinella septempunctata oviposition, which is characterized in that the root of the pea seedlings is set
It is equipped with immersion absorbent cotton.
4. the method according to claim 3 for trapping coccinella septempunctata oviposition, which is characterized in that outside the immersion absorbent cotton
Layer is coated with preservative film.
5. according to the described in any item methods for trapping coccinella septempunctata oviposition of claim 2-4, which is characterized in that the pea is repaired
The quantity of tail aphid is 180-220 heads/plant of pea seedlings.
6. the method according to claim 1-5 for trapping coccinella septempunctata oviposition, which is characterized in that the oviposition carries
Body (8) is PE bags of dark color.
7. the method according to claim 6 for trapping coccinella septempunctata oviposition, which is characterized in that the oviposition carrier (8) is
PE bags of black.
8. the method according to claim 6 or 7 for trapping coccinella septempunctata oviposition, which is characterized in that the oviposition carrier (8)
It is PE bags smooth for black.
9. according to the described in any item methods for trapping coccinella septempunctata oviposition of claim 6-8, which is characterized in that the oviposition carries
Body (8) with a thickness of 0.1-0.2mm.
10. -9 described in any item methods for trapping coccinella septempunctata oviposition according to claim 1, which is characterized in that the pea
The injected volume and the quantity ratio wait trap oviposition ladybug for repairing tail aphid are 500-600:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910403938.0A CN110122426B (en) | 2019-05-15 | 2019-05-15 | Method for trapping coccinella septempunctata to lay eggs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910403938.0A CN110122426B (en) | 2019-05-15 | 2019-05-15 | Method for trapping coccinella septempunctata to lay eggs |
Publications (2)
| Publication Number | Publication Date |
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| CN110122426A true CN110122426A (en) | 2019-08-16 |
| CN110122426B CN110122426B (en) | 2021-11-26 |
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| CN201910403938.0A Expired - Fee Related CN110122426B (en) | 2019-05-15 | 2019-05-15 | Method for trapping coccinella septempunctata to lay eggs |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112243770A (en) * | 2020-10-20 | 2021-01-22 | 四川省烟草公司凉山州公司 | Method for preventing myzus persicae by using field cultivation system |
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| Publication number | Publication date |
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| CN110122426B (en) | 2021-11-26 |
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