CN107279035A - A kind of research method of Predatory Mites to fragrant-flowered garlic maggot predation - Google Patents
A kind of research method of Predatory Mites to fragrant-flowered garlic maggot predation Download PDFInfo
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
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Abstract
The invention discloses a kind of research method of Predatory Mites to fragrant-flowered garlic maggot predation, it is related to plant protection art；By in Predatory Mites and fragrant-flowered garlic maggot access predation test room, carry out the research of predation, and Predatory Mites have been carried out emphatically to the different worm state preferences of fragrant-flowered garlic maggot, functional response of the Predatory Mites to fragrant-flowered garlic maggot, research of the Predatory Mites to the predatory number and Predatory Mites of fragrant-flowered garlic maggot to fragrant-flowered garlic maggot larva predation, predation data of the Predatory Mites to fragrant-flowered garlic maggot are obtained by initiative contrived experiment, and related data is handled, set up predation correlation model, intergrate with practice the accuracy of data verification correlation model, for using microorganism predation mite as medium, biological green prevention and control are carried out to fragrant-flowered garlic maggot valuable reference and premise are provided.Once implementing, not only avoid producing food-safety problem and cause environmental pollution but also use manpower and material resources sparingly, only need to suitably discharge Predatory Mites into mite.
Description
Technical field
The invention discloses a kind of research method of Predatory Mites to fragrant-flowered garlic maggot predation, it is related to plant protection art.
Background technology
Fragrant-flowered garlic maggot, insect name, belongs to Diptera, eye Sciaridae.The lilies such as main harm leek, shallot, onion, spring onion, garlic
Section vegetables, also harm lettuce, green vegetables, celery etc. once in a while are distributed in Beijing, Tianjin, Shandong, Shanxi, Liaoning, Jiangxi, Ningxia, interior
The ground such as Mongolia, Zhejiang, Taiwan, are one of primary pests of allium vegetables.Worm state has adult, ovum, larva, pupa, poly- with larva
Collection is caused harm in the bulb of leek underground part and tender stem.Newly hatched larvae is first caused harm the upper end of leek leaf sheath base and bulb.
Mainly the cause harm young stem of leek of spring, season in autumn two causes rotten, makes fragrant-flowered garlic leaf withered and yellow and dead.The downward activity of summer larva is eaten into bulb,
Severe one bulb rots, and whole pier leek is dead.Existing fragrant-flowered garlic maggot prevention and controls are mainly based on chemical pesticide pouring root, in order to obtain
Good prevention effect, peasant largely uses agricultural chemicals, not only easily produces food-safety problem, is also easy to cause environmental pollution.
And the present invention provides a kind of research method of Predatory Mites to fragrant-flowered garlic maggot predation, using microorganism predation mite as medium, fragrant-flowered garlic maggot is carried out
Predation experiment, determines hobby and predation feature of the Predatory Mites to the different worm states of fragrant-flowered garlic maggot, and carrying out biological green prevention and control for fragrant-flowered garlic maggot provides
Valuable reference and premise, and control effect is expected preferable, implements to save manpower, only need to suitably discharge Predatory Mites into mite i.e.
Can.
The content of the invention
The present invention provides a kind of research method of Predatory Mites to fragrant-flowered garlic maggot predation, and research and utilization Predatory Mites biological control is
It is main, and cultural control, physical control, the fragrant-flowered garlic maggot green prevention and control technology supplemented by chemical prevention can be added, reach green preventing and treating fragrant-flowered garlic maggot
Purpose.
Concrete technical scheme of the present invention is as follows：
A kind of research method of Predatory Mites to fragrant-flowered garlic maggot predation：
Prepare predation test room, by Predatory Mites into mite and fragrant-flowered garlic maggot access predation test room, carried for predation test room
Watering, carries out following study：
Predatory Mites are obtained into mite to the different worm state preference data of fragrant-flowered garlic maggot, the data to acquisition are handled, preyed on
Mite is into hobby sex index of the mite to the different worm state of fragrant-flowered garlic maggot；
Obtain Predatory Mites into functional response data of the mite to fragrant-flowered garlic maggot, the data to acquisition are handled, set up Predatory Mites into
Functional response models of the mite to fragrant-flowered garlic maggot；
Obtain Predatory Mites into predation rate data of the mite to fragrant-flowered garlic maggot, the data to acquisition are handled, set up Predatory Mites into
Mite obtains predation rate to the predatory number model of fragrant-flowered garlic maggot；
Predatory Mites are recorded into mite to fragrant-flowered garlic maggot larva predation, corresponding analysis record is carried out.
Predatory Mites are into acquisition of the mite to fragrant-flowered garlic maggot preference data in described method：By fragrant-flowered garlic maggot according to the classification point of different worm states
Criticizing access, each preys on test room, and each predation test room one starvation time identical Predatory Mites of access are above-mentioned into mite
Each predation test room is put into incubator culture, and the quantity of the different worm states of timing observation fragrant-flowered garlic maggot obtains the different worm state numbers of fragrant-flowered garlic maggot
Measure data.
Fragrant-flowered garlic maggot is divided into six classes according to different worm states in described method：Ovum and first-instar young, ovum and second instar larvae, ovum and
Pupa, first-instar young and second instar larvae, first-instar young and pupa, second instar larvae and pupa.
Predatory Mites are into acquisition of the mite to the functional response data of fragrant-flowered garlic maggot in described method：By fragrant-flowered garlic maggot ovum, first-instar young and
Second instar larvae, sets quantity gradient respectively, each predation test room is accessed, when each predation test room accesses a starvation
Between identical Predatory Mites into mite, while each gradient sets control predation test room, worm state and quantity are answered wherein being only rivals in a contest
Identical fragrant-flowered garlic maggot, incubator culture is put into by each above-mentioned predation test room, and timing observation fragrant-flowered garlic maggot quantity obtains fragrant-flowered garlic maggot quantity
Data.
Fragrant-flowered garlic maggot incremental data is fitted in described method and sets up Functional response models, is obtained according to Functional response models
Predatory Mites are obtained to catch to the daily maximum predatory number of the theory of the different worm states of fragrant-flowered garlic maggot, and using the parameter determination of Functional response models into mite
Mite is eaten into hunting efficiency of the mite to the different worm states of fragrant-flowered garlic maggot.
Predatory Mites are into acquisition of the mite to the predation rate data of fragrant-flowered garlic maggot in described method：By fragrant-flowered garlic maggot ovum, first-instar young, two
Instar larvae is respectively connected in different predation test rooms according to more than the described daily maximum predatory number of theory, each predation experiment
Cell accesses a starvation time identical Predatory Mites into mite, while control predation test room is set, wherein being only rivals in a contest answers worm
State and quantity identical fragrant-flowered garlic maggot, each above-mentioned predation test room are put into incubator culture, setting time gradient observation fragrant-flowered garlic maggot number
Amount, obtains fragrant-flowered garlic maggot incremental data.
Described method is set up and caught according to predatory number, the initial fragrant-flowered garlic maggot quantity accessed is added up in predation time, predation time
Appetite model.
Described method will prey on test room and be placed on and can access one in predation test room under video microscope
Hungry Predatory Mites record predation into mite and fragrant-flowered garlic maggot larva, carry out corresponding analysis record.
The predation test room, is sequentially placed sheet glass, filter paper, the plastic plate for opening up through hole, sheet glass from the bottom up,
Each layer two ends integral retaining is fixed, the via bottoms of plastic plate are completely covered in wherein filter paper and bottom glass piece, and have filter paper
Expose absorption water tariff collection cell humidity in edge.
It is into the process of mite or fragrant-flowered garlic maggot access predation test room by Predatory Mites：Unclamp the folder of predation test room one end
Hold, finger pushes down predation test room top layer sheet glass, slowly level is pushed open, cell portion space is opened, with the writing brush of moistening
Predatory Mites are dipped into mite or fragrant-flowered garlic maggot, quickly chooses into cell, rapidly pushes back top layer sheet glass, cell are closed, then grip
The predation test room one end firmly unclamped.
Usefulness of the present invention：
The present invention provides a kind of research method of Predatory Mites to fragrant-flowered garlic maggot predation, and Predatory Mites and the access predation of fragrant-flowered garlic maggot are real
Test in cell, carry out the research of predation, and carried out Predatory Mites emphatically to the different worm state preferences of fragrant-flowered garlic maggot, Predatory Mites to fragrant-flowered garlic
Research of the functional response, Predatory Mites of maggot to the predatory number and Predatory Mites of fragrant-flowered garlic maggot to fragrant-flowered garlic maggot larva predation, passes through initiative
Contrived experiment obtains predation data of the Predatory Mites to fragrant-flowered garlic maggot, and related data is handled, and sets up predation correlation model, contact
Real data verifies the accuracy of correlation model, is, using microorganism predation mite as medium, biological green prevention and control offer to be carried out to fragrant-flowered garlic maggot
Valuable reference and premise.Once implementing, not only avoid producing food-safety problem and cause environmental pollution but also save people
Power material resources, only need to suitably discharge Predatory Mites into mite.
Brief description of the drawings
Test room structural representation is preyed in Fig. 1 present invention；
Predatory number line chart of Fig. 2 swords hair handkerchief laelaps to the different worm states of fragrant-flowered garlic maggot；
Hunting efficiency line chart of Fig. 3 swords hair handkerchief laelaps to the different worm states of fragrant-flowered garlic maggot；
The schematic flow sheet of Fig. 4 the inventive method.
Brief description of the drawings：1 top layer sheet glass, 2 plastic plates, 3 through holes, 4 filter paper, 5 bottom glass pieces.
Embodiment
The present invention provides a kind of research method of Predatory Mites to fragrant-flowered garlic maggot predation：
Prepare predation test room, by Predatory Mites into mite and fragrant-flowered garlic maggot access predation test room, carried for predation test room
Watering, carries out following study：
Predatory Mites are obtained into mite to the different worm state preference data of fragrant-flowered garlic maggot, the data to acquisition are handled, preyed on
Mite is into hobby sex index of the mite to the different worm state of fragrant-flowered garlic maggot；
Obtain Predatory Mites into functional response data of the mite to fragrant-flowered garlic maggot, the data to acquisition are handled, set up Predatory Mites into
Functional response models of the mite to fragrant-flowered garlic maggot；
Obtain Predatory Mites into predation rate data of the mite to fragrant-flowered garlic maggot, the data to acquisition are handled, set up Predatory Mites into
Mite obtains predation rate to the predatory number model of fragrant-flowered garlic maggot；
Predatory Mites are recorded into mite to fragrant-flowered garlic maggot larva predation, corresponding analysis record is carried out.
With reference to accompanying drawing and specific implementation experimental data, the present invention will be further described.
Research material：
Predatory Mites are from sword hair handkerchief laelaps：The Ministry of Agriculture of Plant Protection institute, Chinese Academy of Agricultral Sciences crop harmful organism is comprehensive
Close and key lab's offer is provided, whole research process all uses into mite；
Fragrant-flowered garlic maggot：The different worm state fragrant-flowered garlic maggots of laboratory rearing.Select representative Dezhou City academy of agricultural sciences experimental plot leek shed
With outdoor cultivation plot, occur more serious spring, Qiu Liangji in annual fragrant-flowered garlic maggot, serious plot occurs for fragrant-flowered garlic maggot, take leek plant
Strain, takes back laboratory with roots and soil.Cultivated, obtain the different worm states of fragrant-flowered garlic maggot.
Each worm state of fragrant-flowered garlic maggot：
Adult, body is elongated, dark brown, and female adult is slightly larger than male worm, and male worm is more active than female adult.
Ovum, ellipse, is initially bright white, slowly becomes milky, have one end to have blackhead appearance when will hatch.
Larva, body is elongated, and nearly maturation larva body is up to 5~7mm, pointed at both ends, the visible alimentary canal of body.
Pupa, exarate pupa 2~3mm of length starts milky, after be changed into yellowish-brown, be grey black before sprouting wings.
Fragrant-flowered garlic maggot larval phase, is most long, and larva is the arch-criminal for endangering plant.
Prepare predation test room：Prepare 30mm × 20mm × 3mm plastic plates 2, the circle of an a diameter of 10mm is made a call in centre
Through hole 3,25mm × 20mm × 1.5mm sheet glass is some, and filter paper 4 is cut into 30mm × 40mm sizes；Predation test room is made,
Bottom glass piece 5, filter paper 4, the plastic plate 2 with manhole 3, top layer sheet glass 1 are sequentially placed from the bottom up, by each layer two ends
Overall to be gripped with sizeable dovetail clip, wherein filter paper 4 and bottom glass piece 5 are completely covered the circle of plastic plate 2 and led to
The bottom of hole 3, the leading edge of filter paper 4 can be flushed with plastic plate 2, and back edge exposes absorption source water and keeps predation test room humidity.
With reference to Fig. 1, it is defined by direction of observation.
Plastic plate, sheet glass, filter paper can adjust shape according to actual conditions, and the through hole of plastic plate can also be opened up as needed
Into other shapes and size, such as through hole opens up the shape for round platform, or top surface section and bottom surface section different shape, such as pushes up
Face section triangle, bottom surface section is circular or ellipse etc..
During by Predatory Mites into mite or fragrant-flowered garlic maggot access predation test room, the dovetail clip of predation test room one end, hand are unclamped
Finger pressure lives to prey on the top layer sheet glass 1 of test room, and slowly level is pushed open, opens cell portion space, is dipped in the writing brush of moistening
Take Predatory Mites into mite or fragrant-flowered garlic maggot, quickly choose into cell, rapidly push back top layer sheet glass 1, close cell, then pressed from both sides with dovetail clip
Hold the predation test room one end for fixing release.
Predatory Mites are into acquisition of the mite to fragrant-flowered garlic maggot preference data：By each 10 of the ovum of fragrant-flowered garlic maggot and first-instar young, access predation
Test room, one starvation of the access hair handkerchief laelaps of the sword of 24 hours is put into the sponge after water suction in crisper into mite, will accessed
The predation test room of fragrant-flowered garlic maggot and mite is placed on sponge, is as preyed on test room and is provided water source, crisper is put into 25 DEG C of trainings
Support in case, the different worm state quantity of fragrant-flowered garlic maggot are observed after 24 hours, aforesaid operations are repeated 10 times.Surveyed with identical method and quantity of sampling quantity
Try sword hair handkerchief laelaps to ovum and second instar larvae, ovum and pupa, first-instar young and second instar larvae, first-instar young and pupa, second instar larvae and
The selectivity of pupa.
Data processing：
Hobby sex index C of the predator to different foods when being coexisted according to a variety of preysiValue judges the hostile prey in day
Preference, formula isQi is predation ratio of the predator to i-th kind of prey in formula, and Fi is i-th kind of prey
Ratio, Ci is predator to the preference of i-th kind of prey.Ci=0 represents that predator does not have preference to i-th kind of prey；0 ＜
Ci ＜ 1 represent that predator has positive preference to i-th kind of prey；- 1 ＜ Ci ＜ 0 represent that predator has negative hobby to i-th kind of prey
Property.
Hobby sex index of the 1 sword hair handkerchief laelaps to each worm state of fragrant-flowered garlic maggot is shown in Table to data above result：
Table 1
Compare hobby sex index (table 1) of the sword hair handkerchief laelaps to fragrant-flowered garlic maggot ovum, first-instar young, second instar larvae and pupa, as a result table
Bright sword hair handkerchief laelaps is maximum to fragrant-flowered garlic maggot first-instar young preference, in the combination of each worm state, in the presence of having first-instar young, to one
Instar larvae predation is more, and other worm states are all shown with negative preference；When ovum and second instar larvae or pupa are combined, to ovum all
Positive preference is shown, second instar larvae is preyed on less, pupa is not preyed on；In the presence of second instar larvae and pupa, to second instar larvae table
Now positive preference, is not preyed on to pupa.Therefore, whole experiment does not find that sword hair handkerchief laelaps preys on the phenomenon of fragrant-flowered garlic maggot pupa, and to other
Three kinds of worm states take food, and predation preference is：First-instar young>Ovum>Second instar larvae>Pupa.
Predatory Mites are into acquisition of the mite to the functional response data of fragrant-flowered garlic maggot：Access fragrant-flowered garlic maggot ovum, an age in predation test room
Larva and second instar larvae, setting gradient is 1,3,5,10,15,20/predation test room, and each predation test room is put into 1
Head starvation 24h sword hair handkerchief laelaps is into mite, while each gradient sets only to be rivals in a contest and answers the fragrant-flowered garlic maggot of worm state to compare, predation experiment is small
Room is placed on the sponge after water suction, is put into crisper, then is placed in 25 DEG C of incubator, and the fragrant-flowered garlic maggot number of predation is observed after 24h
Amount, repeats aforesaid operations 10 times.
Data processing：
Data are fitted using the type disc equations of Holling II：Na=aTtN/(1+aThN), N in formulaaHunted for prey
The quantity of thing；A is attack coefficient；TtThe time for finding prey, i.e. duration of experiment are can be used in for Predatory Mites, in this experiment
Tt=1d, i.e., 24 hour；N is the initial number of prey；ThFor the time needed for 1 prey of disposal；As N → ∞, 1/ThFor reason
By daily maximum predatory number.Attack coefficient a passes through Na=aTtN/(1+aThN) calculate and obtain, set up Functional response models.Wherein
Predatory Mites are N to the Functional response models of fragrant-flowered garlic maggot ovuma=1.0841N/ (1+0.0675N)；First-instar young of the Predatory Mites to fragrant-flowered garlic maggot
Functional response models are Na=1.0735N/ (1+0.0572N)；Predatory Mites are Na to the second instar larvae Functional response models of fragrant-flowered garlic maggot
=1.3173N/ (1+0.4848N)；With reference to Fig. 2 and table 2 sword hair handkerchief laelaps Functional response models and parameter to each worm state of fragrant-flowered garlic maggot.
Wherein Fig. 2 is day predatory number of the sword hair handkerchief laelaps to the different worm states of fragrant-flowered garlic maggot under different fragrant-flowered garlic maggot density, and table 2 is sword hair
Handkerchief laelaps to fragrant-flowered garlic maggot ovum, first-instar young, second instar larvae predation fitting result, it can be seen that sword hair handkerchief laelaps predation
Amount increases with the increase of fragrant-flowered garlic maggot density, when BPH density reaches 10~15, and predatory number increase speed starts to slow down.To table
Each equation carries out Chi-square Test in 2, and it is 0.7538~1.8906, χ 2 to draw χ 2<χ 2 (0.05,4)=9.488, shows theoretical value
Not notable with observation difference, the type disc equations of Holling II of fitting can preferably describe experimental data.Sword hair handkerchief laelaps
Theoretical daily maximum predatory number (1/Th) to fragrant-flowered garlic maggot ovum is 16.05, and the daily maximum of theory to fragrant-flowered garlic maggot first-instar young is preyed on
Measure as 18.76, the daily maximum predatory number of theory to fragrant-flowered garlic maggot second instar larvae is 2.72.
Table 2
Utilize parameter a, T of Functional response modelshCalculate hunting efficiency S, S=of the sword hair handkerchief laelaps to different worm state fragrant-flowered garlic maggots
a/(1+aThN), S is that other specification is identical with fit equation in hunting efficiency, formula.With reference to Fig. 3, sword hair handkerchief laelaps is to fragrant-flowered garlic maggot
The hunting efficiency of different worm states is all reduced with the increase of BPH density.
Predatory Mites are into acquisition of the mite to the predation rate data of fragrant-flowered garlic maggot：Drawn in predation test room according to the above method
The daily maximum predatory number of theory, the fragrant-flowered garlic maggot ovum 20 more than theoretical daily maximum predatory number is respectively put into predation test room
Grain, first-instar young 20, second instar larvae 20, each predation test room are put into 1 starvation 24h sword hair handkerchief laelaps, simultaneously
Answer the predation test room of fragrant-flowered garlic maggot state as control to be only rivals in a contest, each predation test room is placed on the sponge after water suction, is put
Enter in crisper, then be placed in 25 DEG C of incubator, observed respectively after 1h, 3h, 6h, 12h, 18h, 24h in predatory number, repetition
State operation 10 times.
It is i-th hour, adds up predatory number N in the predation time according to predation time taThe as accumulative predation of i-th hour
Amount, the initial fragrant-flowered garlic maggot quantity k=20 of access, set up predatory number model.To the different worms of fragrant-flowered garlic maggot in the sword of reference table 3 hair handkerchief laelaps 24h
The predatory number of state.
Table 3
Predation in sword hair handkerchief laelaps worm state 24h each to fragrant-flowered garlic maggot the results are shown in Table 3, and sword hair handkerchief is calculated by data in table
Laelaps is N to the model of fragrant-flowered garlic maggot ovum predatory number into mitea=20/ (1+e-0.09102t+2.3626), to the mould of fragrant-flowered garlic maggot first-instar young predatory number
Type is Na=20/ (1+e-0.0839t+2.1803), the model to fragrant-flowered garlic maggot second instar larvae predatory number is Na=20/ (1+e-0.1171t+4.0825)。
Table 4 illustrates the predation rate to each worm state of fragrant-flowered garlic maggot in sword hair handkerchief laelaps 24h.
Predatory Mites are all maximum for fragrant-flowered garlic maggot ovum and first-instar young predatory number wherein in 0~3h, predation rate also highest, and
Predatory number and speed to fragrant-flowered garlic maggot second instar larvae are all in this period highest of 3~6h.
Table 4
Predatory Mites are into mite to fragrant-flowered garlic maggot larva predation：Predation test room is placed on can be under video microscope, in predation
The starvation Predatory Mites of 24 hours are accessed in test room into mite and 10 fragrant-flowered garlic maggot larvas, continuous recording predation 24 is small
When, observation sword hair handkerchief laelaps repeats aforesaid operations 5 times to the predation of larva.
Sword hair handkerchief laelaps is placed into behind cell, is that surrounding environment is soundd out first, is typically to be climbed around small chamber interior walls
Row or cell bottom filter paper and top sheet glass Quick-climbing back and forth.After environment is familiar with, slow down creep speed, in small interior
Search of food.When touching fragrant-flowered garlic maggot larva, fragrant-flowered garlic maggot is vised with anterior chelicera, one, which stretches a contracting, assists mouthpart to suck fragrant-flowered garlic maggot larva
Body fluid, fragrant-flowered garlic maggot body can slowly reduce, until being only left black head and body crust.
During above-mentioned utilization the inventive method, for obtain that data carry out repeat, various periods and time
The setting of node can be adjusted according to actual conditions, and said process is only to illustrate technical scheme and carry out
Specific setting, the technical scheme and application of the inventive method can not be limited.
In addition, removing claimed technical solution of the invention, the technology utilized in the present invention is prior art.
Claims (10)
1. a kind of Predatory Mites are to the research method of fragrant-flowered garlic maggot predation, it is characterized in that
Prepare predation test room, by Predatory Mites into mite and fragrant-flowered garlic maggot access predation test room, water is provided for predation test room
Source, carries out following study：
Predatory Mites are obtained into mite to the different worm state preference data of fragrant-flowered garlic maggot, the data to acquisition are handled, obtain Predatory Mites into
Hobby sex index of the mite to the different worm states of fragrant-flowered garlic maggot；
Predatory Mites are obtained into functional response data of the mite to fragrant-flowered garlic maggot, the data to acquisition are handled, and set up Predatory Mites into mite pair
The Functional response models of fragrant-flowered garlic maggot；
Predatory Mites are obtained into predation rate data of the mite to fragrant-flowered garlic maggot, the data to acquisition are handled, and set up Predatory Mites into mite pair
The predatory number model of fragrant-flowered garlic maggot, obtains predation rate；
Predatory Mites are recorded into mite to fragrant-flowered garlic maggot larva predation, corresponding analysis record is carried out.
2. according to the method described in claim 1, it is characterized in that
Predatory Mites are into acquisition of the mite to fragrant-flowered garlic maggot preference data：Fragrant-flowered garlic maggot is accessed into each predation in fact in batches according to the classification of different worm states
Cell is tested, each predation test room accesses a starvation time identical Predatory Mites into mite, each above-mentioned predation test room
Incubator culture is put into, the quantity of the different worm states of timing observation fragrant-flowered garlic maggot obtains the different worm state incremental datas of fragrant-flowered garlic maggot.
3. method according to claim 2, it is characterized in that
Fragrant-flowered garlic maggot is divided into six classes according to different worm states：Ovum and first-instar young, ovum and second instar larvae, ovum and pupa, first-instar young and two
Instar larvae, first-instar young and pupa, second instar larvae and pupa.
4. the method according to claim 1 or 3, it is characterized in that
Predatory Mites are into acquisition of the mite to the functional response data of fragrant-flowered garlic maggot：By fragrant-flowered garlic maggot ovum, first-instar young and second instar larvae, set respectively
Quantity gradient, accesses each predation test room, each predation test room access a starvation time identical Predatory Mites into
Mite, while each gradient sets control predation test room, worm state and quantity identical fragrant-flowered garlic maggot are answered wherein being only rivals in a contest, will be above-mentioned
Each predation test room is put into incubator culture, and timing observation fragrant-flowered garlic maggot quantity obtains fragrant-flowered garlic maggot incremental data.
5. method according to claim 4, it is characterized in that
Fragrant-flowered garlic maggot incremental data is fitted and sets up Functional response models, Predatory Mites are obtained into mite to fragrant-flowered garlic according to Functional response models
The daily maximum predatory number of theory of maggot difference worm state, and using Functional response models parameter determine Predatory Mites into mite to fragrant-flowered garlic maggot not
With the hunting efficiency of worm state.
6. method according to claim 5, it is characterized in that
Predatory Mites are into acquisition of the mite to the predation rate data of fragrant-flowered garlic maggot：By fragrant-flowered garlic maggot ovum, first-instar young, second instar larvae according to more than institute
The daily maximum predatory number of theory stated is respectively connected in different predation test rooms, and each predation test room accesses a starvation
Time identical Predatory Mites, while setting control predation test room, worm state and quantity identical fragrant-flowered garlic are answered wherein being only rivals in a contest into mite
Maggot, each above-mentioned predation test room is put into incubator culture, and setting time gradient observation fragrant-flowered garlic maggot quantity obtains fragrant-flowered garlic maggot quantity number
According to.
7. method according to claim 6, it is characterized in that according to accumulative predatory number, access in predation time, predation time
Initial fragrant-flowered garlic maggot quantity set up predatory number model.
8. according to any described method of claim 1-3 or 5-7, it is characterized in that will predation test room be placed on can record a video it is aobvious
Under micro mirror, one hungry Predatory Mites of access record predation into mite and fragrant-flowered garlic maggot larva in predation test room, carry out phase
Record should be analyzed.
9. the method according to claim 1-8, it is characterized in that the predation test room for preparing is：Put successively from the bottom up
Sheet glass, filter paper, the plastic plate for opening up through hole, sheet glass are put, each layer two ends integral retaining is fixed, wherein filter paper and bottom glass
The via bottoms of plastic plate are completely covered in glass piece, and have filter paper edge to expose absorption water tariff collection cell humidity.
10. method according to claim 9, it is characterized in that Predatory Mites to be preyed on to the mistake of test room into mite or the access of fragrant-flowered garlic maggot
Cheng Wei：The clamping of predation test room one end is unclamped, finger pushes down predation test room top layer sheet glass, and slowly level is pushed open,
Cell portion space is opened, Predatory Mites are dipped with the writing brush of moistening into mite or fragrant-flowered garlic maggot, are quickly chosen into cell, rapidly by top layer glass
Glass piece is pushed back, close cell, then the release that is clamped predation test room one end.
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