CN110115328A - It is a kind of to contain euglena, turmeric and beverage of natural inulin and preparation method thereof - Google Patents

It is a kind of to contain euglena, turmeric and beverage of natural inulin and preparation method thereof Download PDF

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Publication number
CN110115328A
CN110115328A CN201810117425.9A CN201810117425A CN110115328A CN 110115328 A CN110115328 A CN 110115328A CN 201810117425 A CN201810117425 A CN 201810117425A CN 110115328 A CN110115328 A CN 110115328A
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China
Prior art keywords
euglena
beverage
turmeric
pure water
natural inulin
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CN201810117425.9A
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Chinese (zh)
Inventor
蔡冠群
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Langzhi Biotechnology Group
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Langzhi Biotechnology Group
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Priority to CN201810117425.9A priority Critical patent/CN110115328A/en
Publication of CN110115328A publication Critical patent/CN110115328A/en
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38—Other non-alcoholic beverages
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
    • A23L33/22—Comminuted fibrous parts of plants, e.g. bagasse or pulp
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Non-Alcoholic Beverages (AREA)

Abstract

The invention belongs to food processing technology fields, and in particular to a kind of to contain euglena, turmeric and beverage of natural inulin and preparation method thereof.The beverage is to be mixed and stirred for uniformly, by said mixture clarification, removing slag, then be made up of sterilization and sterile filling by euglena mixing magma and pure water by batching step according to formula rate.Beverage color containing euglena, turmeric and natural inulin of the invention is bright, low in calories, low fat, high protein, contains small molecule microelement, is safe and non-toxic, has the effects that anti-oxidant, liver protection protects kidney, relieves the effect of alcohol, solves constipation and quickly regain one's strength.The present invention is prepared for a kind of beverage containing euglena, turmeric and natural inulin by simple method, this beverage had not only remained herbal nutritional ingredient but also had conveniently drunk, and safety, green non-pollution, there is good healthcare function, for being daily drunk by people.

Description

It is a kind of to contain euglena, turmeric and beverage of natural inulin and preparation method thereof
Technical field
The invention belongs to food processing technology fields, and in particular to a kind of drink containing euglena, turmeric and natural inulin Material and preparation method thereof.
Background technique
Euglena is in Euglenophyta, euglena section.Frond be it is unicellular, spindle shape or cylinder and it is slightly flat, by Front end dolly dimple bears elongated flagellum one, moves about whereby.Flagellum base portion nearby has dots in red, can be photosensitive, claims " eyespot ".Its Rich in the substances such as phytoprotein, lipid, acidic polysaccharose, vitamin etc., especially glycoprotein, polysaccharide and nucleic acid.With adding Fast growth and development, enhancing immunity of organisms, the invasion for resisting exotic diseases, promotes personal injury's tissue to repair at activated lymphocyte Again, the effects of cancer-resisting.Taking the euglena drink containing CGF for a long time can mitigate because of the huge sum of money such as the organic matters such as drug, lead, arsenic It is poisoned caused by category, restores the function of detoxification of the internal organs such as liver,spleen,kidney, and gastric ulcer, hypertension and cardiovascular and cerebrovascular can be prevented and treated etc. slow Property disease.
Turmeric also known as Radix Curcumae belong to Zingiber, turmeric, and effect is effect for reducing blood fat, antitumor action, anti-inflammatory and choleretic are anti- Oxidation etc..
Natural inulin is derived from jerusalem artichoke or witloof, is a kind of natural oligofructose and a kind of soluble dietary fiber. Its effect is to control blood lipid, the absorption for reducing blood glucose, promoting minerals, increase intestinal bifidobacteria.
Summary of the invention
The problem to be solved by the present invention is that it is pure and fresh, unique in taste while having to provide a kind of no fishy smell, smell very much again Euglena, turmeric and beverage of natural inulin of high nutritive value and preparation method thereof.The beverage can play healthcare function and Can long-term drinking, be the natural plant beverage that a kind of pair of human body gains everything and lose nothing.Its with anti-oxidant, liver protection shield kidney, relieve the effect of alcohol, solve Constipation and quick the effects of regaining one's strength.
The present invention, which is that the following technical solution is employed, to be realized:
A kind of beverage containing euglena, turmeric and natural inulin, the raw material of the beverage include: euglena powder, ginger Yellow, natural inulin, sweetener, pure water and other food additives.
Further, the mass fraction of the beverage is euglena powder 2%, turmeric 1%, natural inulin 1%, sweetener 5~15%, pure water 80%~90%, other food additives 1%.
Further, the sweetener be one of honey, galactose oligosaccharides, fructose, malic acid, vitamin C or It is a variety of.
Further, the pH value of the pure water is 5.0~7.0.
A kind of preparation method of the beverage as described above containing euglena, turmeric and natural inulin, including following step It is rapid:
(1) deactivation pretreatment: euglena powder, turmeric, natural inulin and pure water are respectively put into container, use is micro- Wave sterilization microwave action 20 seconds or more of 2450 megahertzs, can will be thin when liquid temperature reaches 140 DEG C ± 2 through 3-5 kw of power Born of the same parents bacterium is all killed；
(2) ingredient: by the resulting euglena powder of step (1), turmeric and natural inulin and sweetener and other food additives Add agent to mix, dissolves the effective component in raw material sufficiently by heating and/or stirring means, then insoluble matter is removed, is obtained Magma is mixed to clear euglena；Then pure water is slowly added into euglena mixing magma and stir evenly；
(3) it sterilizes: 135-150 DEG C being carried out to the resulting mixture of step (2), the sterilization of 4-7Secs UHT TRANSIENT HIGH TEMPERATURE；
(4) filling: in purification standard workshop, the resulting mixture of step (3) being subjected to sterile filling.
Further, if generated during euglena mixing magma and pure water mix again in the step (2) Precipitating, also needs further to clarify, precipitating is filtered out.
Further, Peracetic acid sterilization is carried out to packing container in the step (4), then carries out sterile filling.
Further, the packaged form of the beverage can be plastic bottle or glass bottle.
The beneficial effects of the present invention are:
1, the beverage color of the invention containing euglena, turmeric and natural inulin is bright, low in calories, low fat, high egg It is white, containing small molecule microelement, safe and non-toxic, have it is anti-oxidant, liver protection shield kidney, relieve the effect of alcohol, solve constipation and fast quick-recovery body The effects of power.
2, the present invention is prepared for a kind of beverage containing euglena, turmeric and natural inulin by simple method, this Beverage had not only remained herbal nutritional ingredient but also had conveniently drunk, and safety, green non-pollution, had good health care function Can, for being daily drunk by people.
Specific embodiment
In order to which the object of the invention, technical solution is more clearly understood, below invention is further described in detail.
Embodiment one:
(1) deactivation pretreatment: euglena powder, turmeric, natural inulin and pure water are respectively put into container, use is micro- Wave sterilization microwave action 20 seconds or more of 2450 megahertzs, can will be thin when liquid temperature reaches 140 DEG C ± 2 through 3-5 kw of power Born of the same parents bacterium is all killed；
(2) ingredient: by step (1) resulting 2% euglena powder, 1% turmeric and 1% natural inulin and 5% Sweetener and 1% other food additives mix, the sweetener be honey, galactose oligosaccharides, fructose, malic acid, One of vitamin C is a variety of, dissolves the effective component in raw material sufficiently by heating and/or stirring means, then will Insoluble matter removal obtains clear euglena mixing magma；Then being slowly added to 90%, pH value into euglena mixing magma is 5.0~7.0 pure water simultaneously stirs evenly；If producing precipitating again in blending process, also needs further to clarify, will precipitate It filters out；
(3) it sterilizes: 135-150 DEG C being carried out to the resulting mixture of step (2), the sterilization of 4-7Secs UHT TRANSIENT HIGH TEMPERATURE；
(4) in purification standard workshop, the resulting mixture of step (3) is subjected to sterile filling.Wherein, to packing container into The sterilization of row Peracetic acid, then carry out sterile filling；The packaged form of the beverage is unlimited, can be plastic bottle, is also possible to Glass bottle.
Embodiment two:
(1) deactivation pretreatment: euglena powder, turmeric, natural inulin and pure water are respectively put into container, use is micro- Wave sterilization microwave action 20 seconds or more of 2450 megahertzs, can will be thin when liquid temperature reaches 140 DEG C ± 2 through 3-5 kw of power Born of the same parents bacterium is all killed；
(2) ingredient: by step (1) resulting 2% euglena powder, 1% turmeric and 1% natural inulin and 10% Sweetener and 1% other food additives mix, the sweetener be honey, galactose oligosaccharides, fructose, malic acid, One of vitamin C is a variety of, dissolves the effective component in raw material sufficiently by heating and/or stirring means, then will Insoluble matter removal obtains clear euglena mixing magma；Then being slowly added to 85%, pH value into euglena mixing magma is 5.0~7.0 pure water simultaneously stirs evenly；If producing precipitating again in blending process, also needs further to clarify, will precipitate It filters out；
(3) it sterilizes: 135-150 DEG C being carried out to the resulting mixture of step (2), the sterilization of 4-7Secs UHT TRANSIENT HIGH TEMPERATURE；
(4) in purification standard workshop, the resulting mixture of step (3) is subjected to sterile filling.Wherein, to packing container into The sterilization of row Peracetic acid, then carry out sterile filling；The packaged form of the beverage is unlimited, can be plastic bottle, is also possible to Glass bottle.
Embodiment three:
(1) deactivation pretreatment: euglena powder, turmeric, natural inulin and pure water are respectively put into container, use is micro- Wave sterilization microwave action 20 seconds or more of 2450 megahertzs, can will be thin when liquid temperature reaches 140 DEG C ± 2 through 3-5 kw of power Born of the same parents bacterium is all killed；
(2) ingredient: by step (1) resulting 2% euglena powder, 1% turmeric and 1% natural inulin and 15% Sweetener and 1% other food additives mix, the sweetener be honey, galactose oligosaccharides, fructose, malic acid, One of vitamin C is a variety of, dissolves the effective component in raw material sufficiently by heating and/or stirring means, then will Insoluble matter removal obtains clear euglena mixing magma；Then being slowly added to 80%, pH value into euglena mixing magma is 5.0~7.0 pure water simultaneously stirs evenly；If producing precipitating again in blending process, also needs further to clarify, will precipitate It filters out；
(3) it sterilizes: 135-150 DEG C being carried out to the resulting mixture of step (2), the sterilization of 4-7Secs UHT TRANSIENT HIGH TEMPERATURE；
(4) in purification standard workshop, the resulting mixture of step (3) is subjected to sterile filling.Wherein, to packing container into The sterilization of row Peracetic acid, then carry out sterile filling；The packaged form of the beverage is unlimited, can be plastic bottle, is also possible to Glass bottle.
1, experimental material
1.1 test medicines: euglena mixes magma.
1.2 control drugs: lamivudine, oleanolic acid, iron holly bark medicinal substances extract.
1.3 In vitro cell models: the 2.2.15 cell strain (HepG2.2.15 cell) of hepatitis B is transfected.It will HepG2.2.15 cell inoculation (contains 10% fetal calf serum, 380 μ g/mlG418,0.03% glutamine, blueness in DMEM culture solution Mycin and each 100 μ g/ml of streptomysin), set 5%CO2, cultivate in 37 DEG C of incubators.
Above-mentioned control drug saves backup for -20 DEG C through dimethyl sulfoxide (DMSO) after completely dissolution.
2. reagent and key instrument
Dimethyl sulfoxide (DMSO, AR), MTT (thiazolyl blue, AR), hepatitis B virus surface antigen detection kit and B-type hepatitis Malicious e antigen detection kit is purchased from Shanghai Shiye Kehua Biotechnology Co., Ltd.
3. experimental method
The HepG2.2.15 cell of logarithmic growth phase is selected, after tryptic digestive juice digests, diluted concentration is extremely 105Cells/ml, is inoculated in 96 orifice plates, and every 200 μ L of hole after culture for 24 hours, changes the DMEM culture of the mixing magma containing euglena into Liquid, while setting cell controls group without medical fluid culture and drug control group (including lamivudine, oleanolic acid, iron holly bark medicine Material extract), every kind of drug sets 4 parallel holes.Replacement sample liquid is primary within every 3 days, and continuous culture 9 days takes supernatant respectively, makes It is surveyed with hepatitis b virus s antigen diagnostic kit and hepatitis B virus e antigen diagnostic kit according to ELISA method HBsAg and HBeAg content in cell supernatant is determined, while with above-mentioned cell strain, with the cytotoxicity of mtt assay measurement drug.
4. experiment conclusion
As shown in table 1, using HepG2.2.15 cell model, euglena mixing magma of the present invention is able to suppress second The secretion of hepatovirus surface antigen and Hepatitis B virus e antigen has good Anti-HBV activity effect, and effective concentration is low, cytotoxicity It is smaller.In addition, euglena mixing magma is respectively 34.8% He to the inhibiting rate of HBsAg and HBeAg in 100 μ g/ml 35.2%, positive control lamivudine and oleanolic acid and iron holly bark medicinal substances extract are better than to the inhibiting effect of HBV.
Toxicity profile of the table 1 to HepG2.2.15 cell and the inhibitory effect to HBsAg and HBeAg
Note :-: " having no overt toxicity ", which means, detects cell survival rate >=75% with mtt assay；+: " display toxicity " is meant Cell survival rate < 75%.

Claims (8)

1. a kind of beverage containing euglena, turmeric and natural inulin, which is characterized in that the raw material of the beverage includes: a worm Algae powder, turmeric, natural inulin, sweetener, pure water and other food additives.
2. beverage according to claim 1, which is characterized in that the mass fraction of the beverage is euglena powder 2%, ginger Yellow 1%, natural inulin 1%, sweetener 5~15%, pure water 80%~90%, other food additives 1%.
3. beverage according to claim 1, which is characterized in that the sweetener be honey, galactose oligosaccharides, fructose, One of malic acid, vitamin C are a variety of.
4. beverage according to claim 1, which is characterized in that the pH value of the pure water is 5.0~7.0.
5. a kind of preparation side of the beverage containing euglena, turmeric and natural inulin as described in claim 1-4 any one Method, which comprises the following steps:
(1) deactivation pretreatment: euglena powder, turmeric, natural inulin and pure water are respectively put into container, killed using microwave Bacterium microwave action 20 seconds or more of 2450 megahertzs, can be thin by cell when liquid temperature reaches 140 DEG C ± 2 through 3-5 kw of power Bacterium is all killed；
(2) ingredient: by the resulting euglena powder of step (1), turmeric and natural inulin and sweetener and other food additives It mixes, dissolves the effective component in raw material sufficiently by heating and/or stirring means, then insoluble matter is removed, is obtained clear Clear euglena mixes magma；Then pure water is slowly added into euglena mixing magma and stir evenly；
(3) it sterilizes: 135-150 DEG C being carried out to the resulting mixture of step (2), the sterilization of 4-7Secs UHT TRANSIENT HIGH TEMPERATURE；
(4) filling: in purification standard workshop, the resulting mixture of step (3) being subjected to sterile filling.
6. preparation method according to claim 5, which is characterized in that if mixed in euglena former in the step (2) Slurry produces precipitating again during mixing with pure water, also needs further to clarify, precipitating is filtered out.
7. preparation method according to claim 5, which is characterized in that carry out peroxide to packing container in the step (4) Acetic acid sterilization, then carry out sterile filling.
8. preparation method according to claim 7, which is characterized in that the packaged form of the beverage is plastic bottle or glass Glass is bottled.
CN201810117425.9A 2018-02-06 2018-02-06 It is a kind of to contain euglena, turmeric and beverage of natural inulin and preparation method thereof Pending CN110115328A (en)

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CN201810117425.9A CN110115328A (en) 2018-02-06 2018-02-06 It is a kind of to contain euglena, turmeric and beverage of natural inulin and preparation method thereof

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Application Number Priority Date Filing Date Title
CN201810117425.9A CN110115328A (en) 2018-02-06 2018-02-06 It is a kind of to contain euglena, turmeric and beverage of natural inulin and preparation method thereof

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104997824A (en) * 2015-08-04 2015-10-28 陈志高 Euglena health care medicine, euglena health care capsule and preparation method thereof
CN106107267A (en) * 2016-06-24 2016-11-16 湖北丽生堂生物科技有限公司 A kind of composite plant beverage of relieving alcoholic intoxication and supplementary liver nutrition and preparation method thereof
CN106360735A (en) * 2016-10-21 2017-02-01 烟台燕园科玛健康产业有限公司 Food composition assisting in reducing blood sugar and blood fat
CN106387593A (en) * 2016-09-08 2017-02-15 深圳万和制药有限公司 Protein dietary fiber solid beverage
WO2017075262A1 (en) * 2015-10-28 2017-05-04 Algal Scientific Corporation Use of beta-1,3-glucan for modulating immune function and treating intestinal inflammation
US20170290853A1 (en) * 2016-04-12 2017-10-12 Kemin Industries, Inc. Methods to facilitate the solubilization of beta-1,3-glucan and enhance immune function and other related uses
US20170354699A1 (en) * 2016-06-09 2017-12-14 Algaeon, Inc. Euglena lysate composition

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104997824A (en) * 2015-08-04 2015-10-28 陈志高 Euglena health care medicine, euglena health care capsule and preparation method thereof
WO2017075262A1 (en) * 2015-10-28 2017-05-04 Algal Scientific Corporation Use of beta-1,3-glucan for modulating immune function and treating intestinal inflammation
US20170290853A1 (en) * 2016-04-12 2017-10-12 Kemin Industries, Inc. Methods to facilitate the solubilization of beta-1,3-glucan and enhance immune function and other related uses
US20170354699A1 (en) * 2016-06-09 2017-12-14 Algaeon, Inc. Euglena lysate composition
CN106107267A (en) * 2016-06-24 2016-11-16 湖北丽生堂生物科技有限公司 A kind of composite plant beverage of relieving alcoholic intoxication and supplementary liver nutrition and preparation method thereof
CN106387593A (en) * 2016-09-08 2017-02-15 深圳万和制药有限公司 Protein dietary fiber solid beverage
CN106360735A (en) * 2016-10-21 2017-02-01 烟台燕园科玛健康产业有限公司 Food composition assisting in reducing blood sugar and blood fat

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Application publication date: 20190813

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