CN100484531C - Donkey-hide gelatin oral preparation and its making method - Google Patents
Donkey-hide gelatin oral preparation and its making method Download PDFInfo
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- CN100484531C CN100484531C CNB2005100217739A CN200510021773A CN100484531C CN 100484531 C CN100484531 C CN 100484531C CN B2005100217739 A CNB2005100217739 A CN B2005100217739A CN 200510021773 A CN200510021773 A CN 200510021773A CN 100484531 C CN100484531 C CN 100484531C
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Abstract
An orally taken donkey-hide gelatin product is prepared from donkey-hide gelatin through enzymolyzing to obtain polypeptide, and mixing with the pharmacologically acceptable auxiliary.
Description
Technical field
The present invention relates to a kind of donkey-hide gelatin oral preparation, specifically, is to be the oral formulations that feedstock production forms with the Colla Corii Asini.
Background technology
Colla Corii Asini derives from the plucked skin of equine species donkey Equus asinus Linnaeus through decocting, and concentrates the solid gum of making.Main product in Shandong, Zhejiang, Hubei, Beijing, Tianjin, Liaoning.Gather the whole year.Its traditional preparation process method is that donkey skin is floated bubble, and unhairing is cut into small pieces, and floats bubble again and cleans, and the gradation decocting filters, and merging filtrate concentrates (or adding an amount of yellow wine, crystal sugar, Oleum Glycines) to the thick paste shape with slow fire, condensation, and stripping and slicing is dried in the shade.Be rectangle or square bulk, pitchy is glossy.Matter is hard and crisp, and section light, fragment are looked illumination and be brown, and be translucent.With pitch-black, bright, transparent, the no fish-stench of color, not soft person is good through summer.Nature and flavor and function: sweet, flat.Return lung, liver, kidney channel.The YIN nourishing of enriching blood, antiabortive, moisturize hemostasis.Be used for blood deficiency and yellow complexion, dizzy cardiopalmus, the flesh flaccidity is unable, dysphoria and insomnia, stirring-up of pathogenic wind in the interior resulting from deficiency, xeropulmonary cough, chronic cough spitting of blood, haematemesis hematuria, the metrorrhagia of having blood in stool, vaginal bleeding during pregnancy.Usage and dosage is: go into decoction, and 5～15g, molten is converted clothes.
Because the Colla Corii Asini taste is greasy, hinders digestion, the weak careful usefulness of loose stool person of stomach.The preparation that contains Colla Corii Asini at present is more, and many big prescriptions that made up by Colla Corii Asini and other medical material of raw material wherein are as the Colla Corii Asini nourishing blood oral liquid.Because Colla Corii Asini derives from animal skins, its mouthfeel is difficult to accept, therefore, though it is more with the product that Colla Corii Asini single raw material is used as medicine, substantially there is not the oral liquid of directly drinking, in order to overcome its sensory issues, there is report that the Colla Corii Asini crude drug is directly encapsulated, as good fortune board Colla Corii Asini capsule (Shandong Fu A Group Co.,Ltd), it is the capsule that forms through modified form on the basis of instant e-jiao granules, has kept all compositions and whole curative effect of Colla Corii Asini, Colla Corii Asini content 100%, and stable in properties, dosage is accurate, taking convenience, removing solid E-gelatin from smashes, the trouble that molten is taken, and having overcome the limitation of part consumer to instant ass-hide gelatin mouthfeel discomfort, curative effect is rapid, and is safe and reliable.Usage and dosage: oral, one time 3～9,2 times on the one.Taking dose is bigger, and because dosage form is single, can not satisfy clinical demand.The present Colla Corii Asini raw material of still having no way of sets out and solves the report of its sensory issues.
Summary of the invention
In order to address the above problem, technical scheme to be solved by this invention has provided a kind of donkey-hide gelatin oral preparation, specifically, is to be the oral formulations that feedstock production forms with the Colla Corii Asini.Another technical scheme of the present invention has provided the preparation method of this oral formulations.
The invention provides a kind of donkey-hide gelatin oral preparation, it is that containing by Colla Corii Asini is raw material, adds protease hydrolyzed, and the enzymatic hydrolysate of gained is an active component, add the oral formulations that acceptable accessories or complementary composition are prepared from, wherein enzymatic hydrolysate mainly is the following polypeptide of 10KD; The method of Colla Corii Asini enzymolysis is: after Colla Corii Asini is dissolved in phosphate buffer, adjust pH to 7.0-7.5, add the alkaline protease enzymolysis, heated and boiled, adjust pH to 5.0-5.5 acid protease again, enzymolysis, reaction finish to boil, cool off afterreaction liquid successively by filter paper, 0.45 μ m microporous filter membrane, 0.1 μ m microporous filter membrane, the 10KD ultrafilter membrane is adjusted pH to 6.0-7.0, promptly gets the Colla Corii Asini enzymatic hydrolysate.
Wherein, described raw material also comprises Cordyceps mycelium.
Described acid protease is: a kind of or its mixing in pepsin, papain, the aspergillus niger protease; Described alkaline protease is: a kind of or its mixing in trypsin, chymase, the subtilisin.
Described oral formulations is oral liquid, tablet, buccal tablet, capsule, pill.
Described oral liquid pH6.0-7.0.Described oral liquid contains polypeptides matter, its content of peptides〉20mg/ml, total nitrogen content〉8mg/ml, free aminoacid content〉30mg/ml.
The present invention also provides the preparation method of this donkey-hide gelatin oral preparation, and it comprises the steps:
After Colla Corii Asini is dissolved in phosphate buffer, adjust pH to 7.0-7.5, add the alkaline protease enzymolysis, heated and boiled, adjust pH to 5.0-5.5 acid protease again, enzymolysis, reaction finishes to boil, cool off afterreaction liquid and passes through filter paper successively, 0.45 μ m microporous filter membrane, 0.1 μ m microporous filter membrane, the 10KD ultrafilter membrane is adjusted pH to 6.0-7.0, promptly get enzymatic hydrolysate, add the oral formulations that acceptable accessories or complementary composition are prepared from.
Wherein, described acid protease is: a kind of or its mixing in pepsin, papain, the aspergillus niger protease; Described alkaline protease is: a kind of or its mixing in trypsin, chymase, the subtilisin.
Protein raw materials adopts the method for biological enzymolysis such as the relatively poor protease of specificity such as pepsin, trypsin, chymase, papain that collagen protein contained in Cordyceps albumen in the Cordyceps mycelium and the Colla Corii Asini is further degraded among the present invention, and being hydrolyzed into does not have antigenic functional small peptide and aminoacid.Enzymolysis solution can be removed the relatively poor macromole peptide section of absorbability by behind the cross-flow ultrafiltration film bag of molecular cut off 10KD, has preserved the bioactive ingredients less than 10KD: small peptide, aminoacid, polysaccharide, nucleotides and various trace elements.Adopt nanofiltration that enzymolysis solution is concentrated, promptly guaranteed the activity of active component in the solution, again can be according to different size, different content need dilute the high concentration concentrated solution after the nanofiltration, and convenient preparation helps deposit simultaneously again and preserves.Oral formulations of the present invention is especially at child and mid-aged population old, weakness due to chronic disease, the person that is not engaged in mental work for a long time, and the student of nervous study and being in for a long time under the various pressure is in the crowd of sub-health state.Improve immunity, alleviating physical fatigue and raising oxytolerant tolerance, and can promote the skin collagen metabolism, improve moisture of skin.
Oral formulations of the present invention is compared with traditional Colla Corii Asini, adopts enzymolysis, process for purification, has preserved the effect identical with Colla Corii Asini, has overcome the sensory issues of Colla Corii Asini simultaneously, has improved controllability simultaneously, is convenient to clinical use, provides a kind of new selection for clinical.
Obviously, according to foregoing of the present invention,,, can also make modification, replacement or the change of other various ways not breaking away under the above-mentioned basic fundamental thought of the present invention prerequisite according to the ordinary skill knowledge and the customary means of this area.
The specific embodiment of form is described in further detail foregoing of the present invention again by the following examples.But this should be interpreted as that the scope of the above-mentioned theme of the present invention only limits to following example.All technology that realizes based on foregoing of the present invention all belong to scope of the present invention.
The specific embodiment
The preparation of embodiment 1 ass glue oral liquid of the present invention
After the Colla Corii Asini of 1kg is dissolved in the 10L phosphate buffer, adjust pH to 7.0-7.5, add 2% alkaline protease, constantly stir during the enzymolysis and keep pH constant in 55 ℃ of following enzymolysis 4 hours.The 1st enzymolysis finishes post-heating and boils 5 minutes to stop enzyme digestion reaction for the first time.Adjust pH to 5.0-5.5 and also add 2% acid protease, 55 ℃ of reaction 4h, during constantly stir, and keep pH constant.Reaction was boiled 20 minutes after finishing, and the cooling back adds 0.2% Aspartame and stirs, and reactant liquor passes through filter paper successively, 0.45 μ m microporous filter membrane, 0.1 μ m microporous filter membrane, 10KD ultrafilter membrane (millpore company product, the U.S.), adjust pH to 6.8, bottling, 115 ℃ of steam sterilizations.
The preparation of embodiment 2 ass glue oral liquids of the present invention
The 500g Cordyceps mycelium is crushed to 100 orders, adds the 10L phosphate buffer, regulate pH to 8.0-8.5, add 1% trypsin, constantly stir during the enzymolysis and keep pH constant in 35 ℃ of following enzymolysis 5 hours.Finish post-heating and boil 5 minutes with cessation reaction.Be dissolved in the 1kg Colla Corii Asini in this enzymolysis solution and adjust pH to 7.0-7.5, add 2% alkaline protease, constantly stir during the enzymolysis and keep pH constant in 55 ℃ of following enzymolysis 4 hours.The 1st enzymolysis finishes post-heating and boils 5 minutes to stop enzyme digestion reaction for the first time.Adjust pH to 5.0-5.5 and also add 2% acid protease, 55 ℃ of reaction 4h, during constantly stir, and keep pH constant.Reaction was boiled 20 minutes after finishing, and the cooling back adds 0.2% Aspartame and stirs, and reactant liquor passes through filter paper successively, 0.45 μ m microporous filter membrane, 0.1 μ m microporous filter membrane, 10KD ultrafilter membrane (millpore company product, the U.S.), adjust pH to 6.8, bottling, 115 ℃ of steam sterilizations.
The preparation of embodiment 3 medicinal tablets of the present invention
After making reactant liquor by the method for the embodiment of the invention 2, make pale yellow powder by spray dryer, with the 150g powder, add 70g lactose, 5g carboxymethyl starch sodium mix homogeneously, add the 45ml2% carboxylic third methylcellulose aqueous solution and make soft material, granulate 65 ℃ of dryings with 14 mesh sieves, 30 minutes drying times, 16 order granulate add 5g carboxymethyl starch sodium and 1g magnesium stearate, mix homogeneously simultaneously, after measuring content, tabletting.
The preparation of embodiment 4 medicament capsules of the present invention
After making reactant liquor by the method for the embodiment of the invention 2, make pale yellow powder,, add 70g lactose, 10g micropowder silica gel, pack 1 after the mixing into the 150g powder by spray dryer
#In the capsule.
The method of quality control of embodiment 5 ass glue oral liquids of the present invention
The ass glue oral liquid of embodiment 1 preparation is carried out determination of total nitrogen content, and wherein, determination of total nitrogen content adopts the semimicro Kjeldahl; And content of peptides is:
Content of peptides=total nitrogen content * 6.25 one free aminoacid contents
Measurement result is: content of peptides〉20mg/ml, total nitrogen content〉8mg/ml, free aminoacid content〉30mg/ml.
Embodiment 6 drug molecule quantitative determinations of the present invention
The ass glue oral liquid 1ml that gets embodiment 1,2 preparations places the 100ml volumetric flask, add phosphate buffer (pH6.8) to scale, get 100 μ l, SDS one polyacrylate hydrogel electrophoretic determination maximum molecular weight, coomassie brilliant blue R250 dyeing, with the comparison of system standard curve, the result shows that the small peptide maximum molecular weight is 9.8KD in this oral liquid.
Below prove beneficial effect of the present invention by pharmacodynamics test.
Test example 1 ass glue oral liquid of the present invention is to mouse immune organ weight's influence (improving the immunity experiment)
One, test objective
Mice gives oral liquid of the present invention through repeatedly irritating stomach, observes the influence of this medicine to the mouse immune organ weights.
Two, test material
1, tried thing
Oral liquid of the present invention is faint yellow clear liquid, specification: 250mg:20ml, and lot number: 040213, press the preparation of embodiment 1 method, provide by the Yaoyou Science and Technology Development Co., Ltd., Chendu City.
The Colla Corii Asini crude drug, specification: the 500g/ box, lot number: 040215, produce by Donga, Shandong Colla Corii Asini group.
E-jiao hematinic ointment is tan semifluid, specification: the 200g/ bottle, and lot number: 040124, pharmaceutcal corporation, Ltd provides by the four directions, Henan Province.
Artificial cordyceps mycelia's extracting solution is faint yellow clear liquid, specification: the 1g/ bottle, and lot number: 040211, provide by the Yaoyou Science and Technology Development Co., Ltd., Chendu City.
Oral liquid of the present invention, e-jiao hematinic ointment, artificial cordyceps mycelia's extracting solution, facing the time spent is the test desired concn with the normal saline dilution all.The Colla Corii Asini crude drug is pulverized with pulverizer, sieves, and facing the time spent is the test desired concn with the normal saline dilution.
2, experimental animal
Kunming mouse, body weight 18～22g, totally 70, male and female half and half are provided by Sichuan Academy of Medical Sciences institute of lab animals, laboratory animal occupancy permit numbers 119.
3, instrument
Electronic balance
Three, method and result
1, method
Mice is divided into 7 groups at random, 10 every group, male and female half and half.Grouping and dosage see Table 1.Pressed table 1 successive administration 14 days, 1h after the last administration takes off cervical vertebra with mice and puts to death, and opens abdominal cavity and thoracic cavity and wins spleen and thymus respectively, and scales/electronic balance weighing calculates organ index.
Table 1 test grouping and administration
2, result
Result of the test sees Table 2.
The table 2 pair exponential influence of mouse immune organ weights (x ± s)
Annotate: compare * P＜0.05, * * P＜0.01 with the normal control group
Four, conclusion
Oral liquid of the present invention has obvious effect of gain to the mouse immune organ, relatively has utmost point significant difference with the normal control group, with the increase of dosage enhancing trend is arranged, and is certain dose-effect relationship.Oral liquid of the present invention and Colla Corii Asini crude drug compare, and both indifference opposite sex illustrate Colla Corii Asini through enzymolysis, refining, and effective ingredient keeps fully, has brought into play identical effect.
Embodiment 2 ass glue oral liquids of the present invention are to the influence (resisting fatigue experiment) of mice swimming time
One, test objective
Mice gives oral liquid of the present invention through repeatedly irritating stomach, with the influence of medicine to the mice swimming time, observes the antifatigue effect of oral liquid of the present invention.
Two, test material
1, tried thing
Oral liquid of the present invention is faint yellow clear liquid, specification: 250mg:20ml, and lot number: 040213, by embodiment 1 preparation.
The Colla Corii Asini crude drug, specification: the 500g/ box, lot number: 040215, produce by Donga, Shandong Colla Corii Asini group.
E-jiao hematinic ointment is tan semifluid, specification: the 200g/ bottle, and lot number: 040124, pharmaceutcal corporation, Ltd provides by the four directions, Henan Province.
Artificial cordyceps mycelia's extracting solution is faint yellow clear liquid, specification: the 1g/ bottle, and lot number: 040211, provide by the Yaoyou Science and Technology Development Co., Ltd., Chendu City.
Oral liquid of the present invention, e-jiao hematinic ointment, artificial cordyceps mycelia's extracting solution, facing the time spent is the test desired concn with the normal saline dilution all.The Colla Corii Asini crude drug is pulverized with pulverizer, sieves, and facing the time spent is the test desired concn with the normal saline dilution.
2, experimental animal
Kunming mouse, body weight 18～22g, totally 70, male and female half and half are provided by Sichuan Academy of Medical Sciences institute of lab animals, laboratory animal occupancy permit numbers 119.
3, instrument
The glass jar of 50cm * 30cm * 25cm, heavy burden thing (each 2g), thermometer.
Three, method and result
1, method
Mice is divided into 7 groups at random, 10 every group, male and female half and half.Grouping and dosage see Table 3.Pressed table 3 successive administration 14 days, 1h after the last administration, and at the weight of afterbody bundle one 2g.With mice put into respectively glass jar swimming (glass jar adds water, depth of water 20cm, water temperature remains on 20 ± 0.5 ℃.), and reclocking.Can not the person of emerging be muscle power and exhaust that timing has at once exhausted that to muscle power the used time is the mice swimming time from putting into mice when 10 seconds of mouse head submerged.
Table 3 test grouping and administration
2, result
Result of the test sees Table 4.
The influence of table 4 pair mice swimming time (x ± s)
Annotate: compare * P＜0.05, * * P＜0.01 with the normal control group
Four, conclusion
Oral liquid significant prolongation mice swimming time of the present invention, each dosage group and normal control group relatively have utmost point significance and significant difference, obviously prolong with the increase swimming time of dosage, illustrate that oral liquid of the present invention has antifatigue effect.Oral liquid of the present invention and Colla Corii Asini crude drug compare, and both indifference opposite sex illustrate Colla Corii Asini through enzymolysis, refining, and effective ingredient keeps fully, has brought into play identical effect.
Embodiment 3 ass glue oral liquids of the present invention are to the effect of mice normal pressure resistant anoxia (anti-stress experiment)
One, test objective
Mice gives 10KD oral liquid of the present invention through repeatedly irritating stomach, is index with medicine to mice respiratory arrest death time under the normobaric hypoxia condition, observes the resisting oxygen lack of oral liquid of the present invention.
Two, test material
1, tried thing
Oral liquid of the present invention is faint yellow clear liquid, specification: 250mg:20ml, and lot number: 040213, press the preparation of embodiment 1 method, provide by the Yaoyou Science and Technology Development Co., Ltd., Chendu City.
The Colla Corii Asini crude drug, specification: the 500g/ box, lot number: 040215, produce by Donga, Shandong Colla Corii Asini group.
E-jiao hematinic ointment is tan semifluid, specification: the 200g/ bottle, and lot number: 040124, pharmaceutcal corporation, Ltd provides by the four directions, Henan Province.
Artificial cordyceps mycelia's extracting solution is faint yellow clear liquid, specification: the 1g/ bottle, and lot number: 040211, provide by the Yaoyou Science and Technology Development Co., Ltd., Chendu City.
Colla Corii Asini enzymolysis oral liquid, e-jiao hematinic ointment, artificial cordyceps mycelia's extracting solution, facing the time spent is the test desired concn with the normal saline dilution all.The Colla Corii Asini crude drug is pulverized with pulverizer, sieves, and facing the time spent is the test desired concn with the normal saline dilution.
2, experimental animal
Kunming mouse, body weight 18～22g, totally 70, male and female half and half are provided by Sichuan Academy of Medical Sciences institute of lab animals, laboratory animal occupancy permit numbers 119.
3, instrument
200ml ground wide mouthed bottle, stopwatch, sodica calx, vaseline.
Three, method and result
1, method
Mice is divided into 7 groups at random, 10 every group, male and female half and half.Grouping and dosage see Table 5.Pressed table 5 successive administration 14 days, 1h after the last administration puts into the wide mouthed bottle (only putting 1 mice for every bottle) that fills the 15g sodica calx respectively with mice, and is tight with vaseline applicating bottle flap, makes it air tight, immediately reclocking.With the respiratory arrest is index, observes mice because of the dead time of anoxia.
Table 5 test grouping and administration
2, result
Result of the test sees Table 6.
Table 6 pair mice normal pressure resistant anoxia effect (x ± s)
Annotate: compare * P＜0.05, * * P＜0.01 with the normal control group
Four, conclusion
But the time-to-live of oral liquid significant prolongation mice under the normobaric hypoxia condition of the present invention, each dosage group and normal control group relatively have utmost point significance and significant difference, and the increase time-to-live with dosage obviously prolongs, and illustrates that oral liquid of the present invention has the effect that strengthens mice normal pressure resistant anoxia ability.Oral liquid of the present invention and Colla Corii Asini crude drug compare, and both indifference opposite sex illustrate Colla Corii Asini through enzymolysis, refining, and effective ingredient keeps fully, has brought into play identical effect.
The mouthfeel contrast experiment of experimental example 4 oral liquids of the present invention and raw medicinal material Colla Corii Asini
" Donga " board Colla Corii Asini is with the high-quality pure donkey skin, and advantageous Donga groundwater quality adopts consummate scientific technology to boil concentrated forming.According to the Materia Medica of Decoction record, Colla Corii Asini, the thin gas of flavor is thick.Weakness of the spleen and stomach, dyspepsia person causes the loose stool easily.
Oral liquid of the present invention:, add the tender flavor of a small amount of Aspartame and be prepared from through the inventive method enzymolysis, refining.This taste is fresh and sweet, feeble QI perfume (or spice).
Select 20 routine volunteers, be divided into 2 groups at random, every group 10 example.Oral oral liquid of the present invention and Colla Corii Asini (molten is converted clothes with eliminating cold for resuscitation water) carry out the mouthfeel contrast respectively, continuous oral 7 days, and the summary contrast is as follows:
The Colla Corii Asini group: 7/10 volunteer should not accept when oral, and it is heavier mainly to show as abnormal smells from the patient fishy smell, and pharyngeal discomfort is prone to nauseating, regurgitation, vomiting after oral; 3/10 volunteer oral diarrhoea, loose stool occurred after 4 days.
Oral liquid group of the present invention: 10 routine volunteers all easily accept, and think that the little perfume (or spice) of abnormal smells from the patient is fresh and sweet, do not have stench sense.After oral 7 days, gastrointestinal reaction appears in none example.
By functional study and mouthfeel contrast experiment, prove oral liquid determined curative effect of the present invention, orally be accepted easily, and can overcome the defective of traditional Colla Corii Asini, for consumer provides a kind of new selection.
Claims (8)
1, a kind of donkey-hide gelatin oral preparation, it is characterized in that: it is that containing by Colla Corii Asini is raw material, add protease hydrolyzed, the enzymatic hydrolysate of gained is an active component, add the oral formulations that acceptable accessories or complementary composition are prepared from, wherein enzymatic hydrolysate mainly is the following polypeptide of 10KD;
The method of Colla Corii Asini enzymolysis is: after Colla Corii Asini is dissolved in phosphate buffer, adjust pH to 7.0-7.5, add the alkaline protease enzymolysis, heated and boiled, adjust pH to 5.0-5.5 acid protease again, enzymolysis, reaction finish to boil, cool off afterreaction liquid successively by filter paper, 0.45 μ m microporous filter membrane, 0.1 μ m microporous filter membrane, the 10KD ultrafilter membrane is adjusted pH to 6.0-7.0, promptly gets the Colla Corii Asini enzymatic hydrolysate.
2, donkey-hide gelatin oral preparation according to claim 1 is characterized in that: described raw material also comprises Cordyceps mycelium.
3, donkey-hide gelatin oral preparation according to claim 1 and 2 is characterized in that: described acid protease is: a kind of or its mixing in pepsin, papain, the aspergillus niger protease; Described alkaline protease is: a kind of or its mixing in trypsin, chymase, the subtilisin.
4, donkey-hide gelatin oral preparation according to claim 1 and 2 is characterized in that: described oral formulations is oral liquid, tablet, capsule or pill.
5, donkey-hide gelatin oral preparation according to claim 4 is characterized in that: described oral liquid pH6.0-7.0.
6, donkey-hide gelatin oral preparation according to claim 5 is characterized in that: the content of peptides of described oral liquid〉20mg/ml, total nitrogen content〉8mg/ml, free aminoacid content〉30mg/ml.
7, donkey-hide gelatin oral preparation according to claim 4 is characterized in that: described tablet is a buccal tablet.
8, a kind of method for preparing the described donkey-hide gelatin oral preparation of claim 1, it comprises the steps:
After Colla Corii Asini is dissolved in phosphate buffer, adjust pH to 7.0-7.5, add the alkaline protease enzymolysis, heated and boiled, adjust pH to 5.0-5.5 acid protease again, enzymolysis, reaction finishes to boil, cool off afterreaction liquid and passes through filter paper successively, 0.45 μ m microporous filter membrane, 0.1 μ m microporous filter membrane, the 10KD ultrafilter membrane is adjusted pH to 6.0-7.0, promptly get enzymatic hydrolysate, add the oral formulations that acceptable accessories or complementary composition are prepared from.
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| CNB2005100217739A CN100484531C (en) | 2005-09-29 | 2005-09-29 | Donkey-hide gelatin oral preparation and its making method |
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| CN100484531C true CN100484531C (en) | 2009-05-06 |
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| CN101285087B (en) * | 2008-04-30 | 2011-12-07 | 北京华达杰瑞生物技术有限公司 | Polypeptides with donkey hide gelatin and method for preparing same |
| CN108391812A (en) * | 2018-03-06 | 2018-08-14 | 青岛霍中医生物科技有限公司 | A kind of oligomeric ass hide gelatin preparation, preparation method and application |
| CN108338365A (en) * | 2018-04-17 | 2018-07-31 | 青岛霍中医生物科技有限公司 | A kind of donkey-hide gelatin low molecular peptide, preparation method and application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1095902A (en) * | 1994-01-09 | 1994-12-07 | 河南省漯河市第一制药厂 | Cordyceps sinensis and donkey-hide gelatin nutrient solution |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1095902A (en) * | 1994-01-09 | 1994-12-07 | 河南省漯河市第一制药厂 | Cordyceps sinensis and donkey-hide gelatin nutrient solution |
Non-Patent Citations (4)
| Title |
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| 中药口服液近年的概况与展望. 刘文成，胡子全.中国中药杂志，第16卷第8期. 1991 |
| 中药口服液近年的概况与展望. 刘文成，胡子全.中国中药杂志，第16卷第8期. 1991 * |
| 蛋白酶水解驴皮制备多肽口服液的研究. 陆晓滨，董贝磊，赵祥忠，刘庆军.中国食品添加剂，第1期. 2003 |
| 蛋白酶水解驴皮制备多肽口服液的研究. 陆晓滨，董贝磊，赵祥忠，刘庆军.中国食品添加剂，第1期. 2003 * |
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