CN105434895A - Gastrodia elata rhodiola rosea cream and preparation method thereof - Google Patents
Gastrodia elata rhodiola rosea cream and preparation method thereof Download PDFInfo
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Abstract
The present invention discloses gastrodia elata rhodiola rosea cream for enhancing immunity and a preparation method thereof, the gastrodia elata rhodiola rosea cream includes gastrodia elata, rhodiola rosea and fructose. Functional experiments in mice show that the gastrodia elata rhodiola rosea cream has the effect of enhancing the immunity. Compared with the prior art: the gastrodia elata rhodiola rosea cream is safe and effective, simple in prescription, easy in production process and suitable for industrial production.
Description
Technical field
The present invention relates to a kind of Rhizoma Gastrodiae Herba hylotelephii erythrosticti cream and preparation method thereof, belong to field of health care products.
Background technology
Immunity is generally also resistance, the body immunity usually said be exactly health when being subject to external infringement, such as when antibacterial, poisoning intrusion human body, the ability of body resistance exotic invasive.Modern medicine thinks that immune function has three aspects: first is immunity epidemic prevention, immunne response is there is in him to antigenic substance (pathogenic microorganism, vaccine, toxoid, foreign protein etc.), final elimination antigenic substance, play the effect of infection immunity, body immunity carrys out self-immunity systems, and when human body lacks effectively exercise time, immunity declines unavoidably, cause easily often feeling tired, show that work is often felt tired spiritless; Catch a cold, wound easily infects, and gastrointestinal function declines, easily by infectious disease attack etc.Along with the progress of society, the quickening of people's rhythm of life, the change of living environment, and the increasing pressure of each side is large, causes the crowd of immunity degradation to get more and more.In the case, very easily cause the infection such as antibacterial, virus, fungus, therefore hypoimmunity the most directly shows is exactly liable to illness.Because of often ill, increased the weight of the consumption of body, thus generally have a delicate constitution, the reduction of malnutrition, lethargy, fatigue and weak, appetite, the performance such as sleep disorder, sick, have an injection and take medicine the normal potluck that just gets married.At every turn sickly all to could to recover for a long time, and usually recurrent exerbation.If things go on like this can cause health and intelligent development bad, also easily bring out major disease.Therefore, need to carry out enhancing immunity by external food or health product.Food enhancing immunity is not good.
At present, more people select to adopt tcm product enhancing immunity, with for enanthrope in product, by regulating the therapeutic modality of human body general body state comparatively effective.The patent No. be CN201210506240.X disclose a kind ofly adopt Radix Rhodiolae, Rhizoma Chuanxiong, Rhizoma Gastrodiae, Radix Notoginseng 4 kinds of Chinese drug preparations can the health product of enhancing immunity, its Chinese crude drug kind used is relatively many, the interaction do not expected between medicine may be caused, the extraction comparatively caused complicated process of preparation of difficulty of active substance, cost increase.
Summary of the invention
Object of the present invention provides a kind of unguentum of enhancing immunity, and described unguentum determined curative effect, prescription is simple, processing technology is easy, be suitable for industrialized great production.
Particularly, the unguentum of described a kind of enhancing immunity, calculates according to composition by weight, is prepared from by following raw material medicaments and adjuvant:
100 ~ 405 parts, Rhizoma Gastrodiae, Radix Rhodiolae 135 ~ 450 parts, 80 ~ 210 parts, fructose.
Preferably, calculate according to composition by weight, be prepared from by following raw material medicaments and adjuvant:
185 ~ 300 parts, Rhizoma Gastrodiae, Radix Rhodiolae 200 ~ 350 parts, 100 ~ 180 parts, fructose.
More preferably, calculate according to composition by weight, be prepared into unguentum by following raw material medicaments and adjuvant:
220 parts, Rhizoma Gastrodiae, Radix Rhodiolae 250 parts, 145 parts, fructose.
Another object of the present invention provides a kind of unguentum preparation method of enhancing immunity, it is characterized in that, comprises the following steps:
(1) Rhizoma Gastrodiae, Radix Rhodiolae raw material powder are broken into coarse powder, cross 20-50 mesh sieve, mix homogeneously, load extraction pot, add raw material 8-12 95% food grade ethanol doubly;
(2) open microwave source when being preheating to about 40 DEG C, be adjusted to required microwave power, make coarse powder accept microwave radiation in flow process;
(3) filter, filtering residue can extract repeatedly; Extract complete, after filtrate reduced in volume, obtain thick product;
(4) by above-mentioned aqueous extract 70-80 DEG C, vacuum be evaporated to 60 DEG C under being 0.08Mpa condition time relative density be 1.20-1.30, obtain extractum;
(5) in 100,000 grades of clean control zones, getting fructose adds in above-mentioned extractum, and 60-70 DEG C is uniformly mixed 30 minutes, to dissolving mix homogeneously completely, obtains semi-finished product cream, semi-finished product cream 60-70 DEG C heat is loaded, specification 60g/ bottle, 105 DEG C of sterilizings 45 minutes, cooling, inspection, to obtain final product.
Beneficial effect of the present invention:
Rhizoma Gastrodiae: sweet in the mouth, flat, return Liver Channel.Endogenous wind stopping relieving convulsion, suppressing liver-YANG, dispelling wind and removing obstruction in the collateral.Dizzy for having a headache, numb limbs and tense tendons, infantile convulsion, epilepsy clonus, tetanus.In Rhizoma Gastrodiae, gastrodia elata polysaccharide can enhancing human body immunity power, improves the nutrition of cardiac muscle and brain, raising hypoxia-bearing capability.
Radix Rhodiolae: sweet is bitter, flat.Return lung, heart channel.For benefiting QI for activating blood circulation, promote blood circulation and relieving asthma.Blood stasis due to qi deficiency, obstruction of qi in the chest and cardialgia, apoplectic hemiplegia, asthenia is panted.Radix Rhodiolae has enhancing cerebral blood flow, the effect improving immunity of organisms and the psychological effect of increasing work efficiency.
Fructose: fructose mainly uses as sweeting agent in food.Its sugariness is 1.8 times of sucrose, is the sugar that in all natural sugar, sugariness is the highest, so under same sweetness standard, the intake of fructose is only the half of sucrose.And with synthesis for compared with sugar, fructose is then more reliable on trophism and safety.Can say, fructose is one of current safe, the most healthy known in the world sugar.
More than comprehensive, the 2 taste Chinese crude drugs that it is monarch drug that unguentum of the present invention only uses with Rhizoma Gastrodiae and Radix Rhodiolae, utilize the nutrition of the improvement cardiac muscle of Rhizoma Gastrodiae and brain, improve hypoxia-bearing capability, with the strong cerebral blood flow of Radix Rhodiolae, the effect improving immunity of organisms and the psychological effect of increasing work efficiency, the mutual synergism of active component of each Chinese medicine, enhancing human body immunity power effectively, fructose taste masking is granulated in addition, makes this product assurance mouthfeel and is easy to produce.
In order to make those of ordinary skill in the art better understand the present invention, the applicant has carried out series of animal experiments research, to prove effect of the present invention:
One, pharmacodynamic study
Experimental example 1 Rhizoma Gastrodiae Herba hylotelephii erythrosticti of the present invention cream improves the animal experiment of immunity of organisms
1, material:
Medicine:
Treatment group medicine: Rhizoma Gastrodiae Herba hylotelephii erythrosticti cream A1, A2, A3, A4, A5, A6, A7 of preparing according to method described in embodiment 1-7.
Contrast 1 group of medicine: happy family spreads Rhizoma Gastrodiae Capsulae Radix Panacis Quinquefolii (Nanjing Tongrentang Pharmaceutical Co., Ltd.) always.
Contrast 2 groups of medicines: according to the patent No. for method described in CN201210506240.X obtains unguentum.
Blank group: normal saline.
Animal: 480 Healthy female ICR mices, body weight 18-22g, tests mice original body mass between each group balanced.
2, metering is selected and is given tested material approach:
Take A1-A72.0g, contrast 1 group of medicine 0.5g, contrast 2 groups of medicine 1.0g and be assigned to 20mL with distilled water respectively, each group of mice all presses the continuous per os gavage of 0.1mL/10g dosage every day, blank group gavage equal-volume normal saline, after 30d, surveys every immune indexes.
3, method:
Test mice is divided into 4 groups, and immune one group is carried out carbonic clearance experiment; Internal organs/body weight ratio, delayed allergy (DTH) are carried out in immunity two groups, antibody-producting cell detects and serum hemolysin measures; Immunity three groups is carried out Turnover of Mouse Peritoneal Macrophages and is engulfed chicken red blood cell experiment; Mouse lymphocyte transformation experiment and the experiment of NK cytoactive detection of ConA induction are carried out in immunity four groups.Each immune group mice is divided into treatment group (A1, A2, A3, A4, A5, A6, A7), contrasts 1 group, contrasts 2 groups, blank group, totally 10 groups, mice needed for every a small group 12.
3.1 immune one group:
On mice carbonic clearance impact test: the india ink diluted mouse tail vein injection 1:3, treat that prepared Chinese ink injects timing immediately, to inject after prepared Chinese ink 2,10min, get blood 20 μ L from angular vein clump respectively, and be added to 2mLNa
2cO
3in solution, sentence Na with ultraviolet-uisible spectrophotometer at 600nm wavelength
2cO
3solution makes blank densitometric value (OD).By sacrifice, get liver and spleen is weighed, calculate phagocytic index.
3.2 immune two groups:
3.2.1 to mice organs/body weight ratios affect experiment: put to death animal after mice organs injection SRBC, 5d, get thymus, spleen is weighed, calculate internal organs/body weight ratio.Prepare splenocyte suspension simultaneously, carry out antibody-producting cell mensuration.
3.2.2 mice delayed allergy is tested: mouse peritoneal injection 2% (v/v) SRBC, after sensitization, 4d measures left back sufficient sole of the foot thickness, then at measuring point subcutaneous injection 20% (v/v) SRBC, every Mus injects 20 μ L, after injection, 24h measures left back sufficient sole of the foot portion thickness, same position measures 3 times, gets average.To attack the degree that the sufficient sole of the foot thickness difference (swelling degree of the paw) in front and back represents DTH.
3.2.3 to mouse antibodies cellulation test experience: get spleen, make cell suspension.Hanks liquid double with equivalent after the culture medium heating for dissolving of top layer is mixed, subpackage small test tube, often pipe 0.5ml, 50 μ L10%SRBC (v/v are added again in pipe, with SA liquid preparation), 20 μ L splenocyte suspensions, after rapid mixing, be poured on the slide of brush agarose thin layer, after agar solidification, fragmentation level button is placed on slide frame, puts into CO2 incubator incubation 1.5h, then join in slide frame groove with the complement (1:8) of SA liquid dilution, after continuing incubation 1.5h, counting hemolysis plaque number.
3.2.4 Mouse Blood is purified the blood lysin test experience: after mice organs injection SRBC5d, pluck eyeball and get blood, centrifuging and taking serum-dilution 100 times, carries out HD50 pH-value determination pH.Then put to death animal, get thymus, spleen is weighed, calculate internal organs/body weight ratio.Prepare splenocyte suspension simultaneously, carry out antibody-producting cell mensuration.
3.3 immune three groups:
3.3.1 Turnover of Mouse Peritoneal Macrophages engulfs chicken red blood cell test: mouse peritoneal injects 20% chicken erythrocyte suspension 1mL, and interval 30min puts to death, and is fixed on Mus plate, cut off abdominal skin, injecting normal saline 2mL, rotate Mus plate 1min, sucking-off abdominal cavity washing liquid 1mL, divides and drips on 2 slides, 37 DEG C of incubation 30min, use normal saline rinsing, dry, fix with 1:1 acetone methanol solution, Giemsa dye liquor dyeing 3min, dry with distilled water rinsing, with oily mirror microscopy, calculate phagocytic rate and phagocytic index.
3.4 immune four groups:
3.4.1 mouse spleen lymphocyte transformations affect is tested: asepticly get spleen, prepare splenocyte suspension, wash 2 times with Hanks liquid, each centrifugal 10min (1000r/min), by cell suspension in 1mLRPMI1640 complete culture solution, adjustment cell concentration is 3 × 10
6individual/mL.Add in 24 well culture plates by a cell suspension point holes, every hole 1mL, a hole adds 75 μ LConA liquid, and 5%CO in contrast, is put in another hole
2, cultivate 72h in 37 DEG C of incubators.Cultivation terminates front 4h, every hole Aspirate supernatant 0.7mL, adds 0.7mL not containing the RPMI1640 complete culture solution of calf serum, adds MTT50 μ L simultaneously, continues to cultivate 4h.After cultivation terminates, every hole adds 1mL acid isopropyl alcohol, and piping and druming evenly, makes purple crystal dissolve completely, measures OD value, deduct the OD value not adding ConA hole represent lymphopoiesis ability with the OD value adding ConA hole at 570nm wavelength place.
3.4.2 to NK cells in mice active function: before experiment, 24h is by target cell Secondary Culture, washing 3 times before application with Hanks liquid, is 4 × 10 with RPMI1640 complete culture solution adjustment cell concentration
5individual/mL.Mice cervical dislocation is put to death, and asepticly gets spleen, prepares splenocyte suspension, washes 2 times with Hanks liquid, each centrifugal 10min (1000r/min).Abandon supernatant cytoplasm is upspring, add 0.5mL aquesterilisa splitting erythrocyte, 0.5mL2 times of Hanks liquid and 8mLHanks liquid is added again after 20s, centrifugal 10min (1000r/min), resuspended containing the RPMI1640 complete culture solution of 10% calf serum with 1mL, with counting after 1% glacial acetic acid dilution, adjustment cell concentration is 2 × 10
7individual/mL.Target cell is added 96 well culture plates, every hole 100 μ L, test hole adds 100 μ L splenocytes (effect target is than 50:1), and Spontaneous release hole adds 100 μ L culture fluid, maximum release aperture adds 100 μ L1%NP40, cultivate 4h for 37 DEG C, centrifugal, get supernatant 100 μ L and put in 96 hole ELISA Plate, add LDH matrix liquid 100 μ L, reaction 6min, with the HCL cessation reaction of 1mol/L, in microplate reader 490nm place measured value OD value.
4 results:
4.1 body weight change situations:
Before testing, all mices all take body weight value; Mice to accept after tested material 30 days, all mices all take body weight value again, because mice original body mass is balanced, after accepting tested material, body weight is also without significant difference, treatment group (A1, A2, A3, A4, A5, A6, A7) compares with blank group, P>0.05, thus invention formulation on Mouse Weight without impact.
4.2 immunity measurement results:
According to " health-care food evaluation and technical specification " relevant criterion, now experiment acquired results is divided into: internal organs/weight ratio pH-value determination pH, comprises spleen/body weight ratio, thymus thymus/body weight ratio, the results are shown in Table 1; Cellular immune function assay, comprises the experiment of mouse spleen lymphocyte transformations affect, to mice delayed allergy experiment, the results are shown in Table 2; Humoral immune function measures, and comprises mouse antibodies cellulation test experience, Mouse Blood purifies the blood lysin test experience, the results are shown in Table 3; Monocytes/macrophages functional examination, comprises and engulfs chicken red blood cell test to mice carbonic clearance impact test, Turnover of Mouse Peritoneal Macrophages, the results are shown in Table 4; NK cytoactive detection, the results are shown in Table 5.
Each group of table 1 is on the impact of mice organs/body weight ratio
| Group | Spleen/body weight ratio | Thymus/body weight ratio |
| A1 group | 0.50±0.07 | 0.23±0.05 |
| A2 group | 0.52±0.05 | 0.23±0.01 |
| A3 group | 0.52±0.08 | 0.24±0.03 |
| A4 group | 0.50±0.03 | 0.22±0.02 |
| A5 group | 0.52±0.01 | 0.23±0.05 |
| A6 group | 0.51±0.06 | 0.22±0.05 |
| A7 group | 0.53±0.05 | 0.23±0.07 |
| Matched group 1 | 0.53±0.02 | 0.25±0.08 |
| Matched group 2 | 0.53±0.09 | 0.24±0.05 |
| Blank group | 0.51±0.01 | 0.23±0.06 |
Result: each group of impact on mouse spleen/body weight ratio: invention formulation group (A1-A5) compares with blank group, P>0.05; With contrast 1 group and compare, P>0.05; With contrast 2 groups and compare, P>0.05.
The each group of impact on mouse thymus/body weight ratio: invention formulation group (A1-A5) compares with blank group, P>0.05; With contrast 1 group and compare, P>0.05; With contrast 2 groups and compare, P>0.05.
Conclusion: invention formulation on mice organs/body weight ratio without impact.
Each group of table 2 is to mouse cell immunity function result
| Group | Lymphocyte proliferation ability (OD difference) | Swelling degree of the paw |
| A1 group | 0.56±0.08#* | 0.37mm±0.04mm#* |
| A2 group | 0.62±0.03#* | 0.41mm±0.03mm#* |
| A3 group | 0.64±0.06#* | 0.43mm±0.06mm#* |
| A4 group | 0.58±0.08#* | 0.37mm±0.05mm#* |
| A5 group | 0.54±0.09#* | 0.39mm±0.08mm#* |
| A6 group | 0.42±0.09△ | 0.27mm±0.03mm△ |
| A7 group | 0.65±0.09#* | 0.45mm±0.08mm#* |
| Contrast 1 group | 0.46±0.12 | 0.28mm±0.19mm |
| Contrast 2 groups | 0.48±0.15 | 0.29mm±0.23mm |
| Blank group | 0.27±0.09 | 0.21mm±0.13mm |
Remarks: lymphocyte proliferation ability: invention formulation group (A1-A5) compares with blank group, #P < 0.01, △ P < 0.05; With contrast 1,2 group and compare, * P < 0.05.
Swelling degree of the paw: invention formulation group (A1-A5) compares with blank group, #P < 0.01; With contrast 1,2 group and compare, * P < 0.05.
Conclusion: invention formulation is positive to mouse cell immunity test result, effect is better than contrast 1,2 groups.In addition, adopt the A6 group of low component formula, its therapeutic effect no significant difference compared with matched group, and high component A7 group effect compared with A1-A5 group of the present invention is not significantly increased, show to adopt amount ranges of the present invention, respond well in enhancing mouse cell immunologic function, wherein best composition is A3 group.
Each group of table 3 is to mouse humoral immune function result
| Group | Hemolysis plaque number (× 10 3/ full spleen) | Half hemolysis value (HC 50) |
| A1 group | 25±3# | 59±11# |
| A2 group | 32±2#* | 64±13#* |
| A3 group | 31±5#* | 63±15#* |
| A4 group | 26±3#* | 61±14#* |
| A5 group | 28±5#* | 60±13#* |
| A6 group | 19±2△ | 54±11△ |
| A7 group | 31±5#* | 63±16#* |
| Contrast 1 group | 22±7 | 56±19 |
| Contrast 2 groups | 20±9 | 48±21 |
| Blank group | 14±6 | 40±14 |
Remarks: hemolysis plaque number: invention formulation group (A1-A5) compares with blank group, #P < 0.01, △ P < 0.05; With contrast 1,2 group and compare, * P < 0.05.
Half hemolysis value: invention formulation group (A1-A5) compares with blank group, #P < 0.01, △ P < 0.05; With contrast 1,2 group and compare, * P < 0.05.
Conclusion: the present invention is positive to mouse humoral immune result of the test, effect is better than contrast 1,2 groups.In addition, adopt the A6 group of low component formula, its therapeutic effect no significant difference compared with matched group, and high component A7 group effect compared with A1-A5 group of the present invention is not significantly increased, show to adopt amount ranges of the present invention, respond well in enhancing mouse humoral immune function aspects, wherein best composition is A2 group.
Each group of table 4 affects result to mouse monokaryon-macrophage function
Remarks: carbonic clearance ability phagocytic index: invention formulation group (A1-A5) compares with blank group, #P < 0.01, △ P < 0.05; With contrast 1,2 group and compare, * P < 0.05.
Half hemolysis value: engulf chicken red blood cell ability phagocytic percentage and compare with blank group with phagocytic index (A1-A5), #P < 0.01, △ P < 0.05; With contrast 1,2 group and compare, * P < 0.05.
Conclusion: the present invention is positive to mouse monokaryon-macrophage function result of the test, effect is better than contrast 1,2 groups.In addition, adopt the A6 group of low component formula, its therapeutic effect no significant difference compared with matched group, and high component A7 group effect compared with A1-A5 group of the present invention is not significantly increased, show to adopt amount ranges of the present invention, respond well in enhancing mouse monokaryon-macrophage function, wherein best composition is A2 group.
Each group of table 5 affects result to NK cells in mice activity
| Group | NK cytoactive (%) |
| A1 group | 56.7±10.8 |
| A2 group | 70.4±10.3#*△ |
| A3 group | 68.9±12.0#*△ |
| A4 group | 63.3±11.2#△ |
| A5 group | 65.8±11.7#*△ |
| A6 group | 54.1±11.3 |
| A7 group | 68.3±11.2#*△ |
| Contrast 1 group | 55.3±13.8 |
| Contrast 2 groups | 51.3±12.3 |
| Blank group | 47.1±10.8 |
Result: invention formulation group (A1-A5) compares with blank group, #P < 0.01, △ P < 0.05; With contrast 1,2 group and compare, * P < 0.05.
Conclusion: the present invention is positive to NK cells in mice activity influence result of the test, effect is better than contrast 1,2 groups.In addition, adopt the A6 group of low component formula, its therapeutic effect no significant difference compared with matched group, and high component A7 group effect compared with A1-A5 group of the present invention is not significantly increased, show to adopt amount ranges of the present invention, respond well in enhancing NK cells in mice is active, wherein best composition is A2 group.
Overall experimental result shows, Rhizoma Gastrodiae Herba hylotelephii erythrosticti cream can improve mice spleen lymphocyte proliferation ability, increase antibody-producting cell number, improve serum hemolysin, strengthen that Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell ability, strengthened mouse monokaryon-macrophage phagocytic function, to strengthen NK cells in mice active; On spleen/body weight ratio, thymus/body weight ratio, swelling degree of the paw without impact; And successful is better than contrast 1 group and contrasts 2 groups, illustrate that the action effect of Rhizoma Gastrodiae Herba hylotelephii erythrosticti cream enhancing immunity is good.
The stability of experimental example 2 Rhizoma Gastrodiae Herba hylotelephii erythrosticti of the present invention cream and toxicity test
2.1, accelerated stability test: get the sample A1-A5 that embodiment of the present invention 1-5 is obtained; Temperature 40 DEG C ± 2 DEG C, place under the condition of relative humidity 75% ± 5%, once investigate in duration of test 1st month, 2 months, 3 months, 6 samplings at the end of month respectively.With outward appearance, denseness, abnormal smells from the patient, with or without offscum and sand return, effective active component content, relative density, insoluble matter and health examination for index, check the stability of product, result shows: compound recipe microemulsion prepared by the present invention is stored under accelerated test condition, each investigation project is showed no exception, there is not significant change in active constituent content yet, its relative density, insoluble matter and health examination all should meet the pertinent regulations under existing " Chinese Pharmacopoeia " (one) annex soft extract item, all meet quality criteria requirements.
2.2, sample A1-A5 obtained for embodiment of the present invention 1-5 is carried out toxicological experiment research, through zoopery, body weight without significant change, be a cup too low depressed, movable to reduce or other symptom occurs.Prove that unguentum agent toxic and side effects of the present invention is little, be very beneficial for Clinical practice.
Test Summary: test shows that preparation of the present invention is nontoxic.
To sum up, with the Rhizoma Gastrodiae Herba hylotelephii erythrosticti cream that the inventive method is obtained, through zoopery checking, result shows medicine enhancing immunity determined curative effect of the present invention, and safety is good.
Two, technical study
Experimental example 3 extracting method is selected
Get Rhizoma Gastrodiae 220g, Radix Rhodiolae 260g, be pulverized and mixed, adopt that water extract-alcohol precipitation, ethanol extraction, water extraction leave standstill, microwave extraction four kinds of methods extract respectively, with Determination of Salidroside, extracting solution color and luster, clarity, filtration complexity in the gastrodin content in Rhizoma Gastrodiae, Radix Rhodiolae for index, investigate the extraction ratio of four kinds of methods, the results are shown in following table:
Table 6 extracting method is selected
| Method | Evaluation index | Gastrodin content | Determination of Salidroside | Clarity | Filter difficulty or ease |
| 1 | Water extract-alcohol precipitation | 5.85mg/g | 4.49mg/g | Clear and bright | Easily |
| 2 | Ethanol extraction precipitate with ethanol | 5.36mg/g | 5.47mg/g | Clear and bright | Difficult |
| 3 | Water extraction leaves standstill | 5.14mg/g | 3.86mg/g | Clear and bright | Easily |
| 4 | Microwave extraction | 6.87mg/g | 11.73mg/g | Clear and bright | Easily |
Experimental result shows, four kinds of method extracting solution are all more transparent, adopts that water extract-alcohol precipitation, water extraction leave standstill, the method for microwave extraction is filtered and is all easier to, and the effective ingredient wherein obtained with the method for microwave extraction is the highest, extracts more complete.Therefore the extracting method of preferred microwave extraction.
The adjuvant of experimental example 4 unguentum is selected
The choice experiment of 4.1 filleies
(1) because oral pastes require that mouthfeel is good, be easy to preparation, the adjuvant that general unguentum is selected has xylitol, motosada sugar, fructose, brown sugar etc., we are by the research of the inspection target such as mouthfeel, preparation situation, cost effectiveness to the product after obtaining through these five kinds of filleies, select one or more mixture most suitable.The results are shown in following table:
Table 7 filler Selection experiment
| Adjuvant | Mouthfeel | Be easy to unguentum processed | Economical |
| Xylitol | Better | Easier | More expensive |
| Motosada sugar | Good | Easier | Expensive |
| Fructose | Good | Easily | Cheaply |
| Brown sugar | Better | Not easily | Cheaply |
By experiment, after considering indices, can find out that fructose melting is good, be easy to granulate, economy is high.
Below, enumerate embodiment and the present invention is further described, but the present invention is not limited to following embodiment.
Detailed description of the invention:
Embodiment 1: the preparation of Rhizoma Gastrodiae Herba hylotelephii erythrosticti cream
(1) raw material is taken according to the following ratio: Rhizoma Gastrodiae 100g, Radix Rhodiolae 135g, fructose 80g
(2) Rhizoma Gastrodiae, Radix Rhodiolae raw material powder are broken into coarse powder, cross 40 mesh sieves, mix homogeneously, load extraction pot, add 95% food grade ethanol of raw material 8 times, when being preheating to 40 DEG C, open microwave source, be adjusted to required microwave power, make coarse powder accept microwave radiation in flow process, filter, filtering residue can extract repeatedly; Extract complete, after filtrate reduced in volume, obtain thick product, be 1.25 (60 DEG C of mensuration) by above-mentioned aqueous extract concentrating under reduced pressure (70 DEG C, vacuum is 0.08Mpa) to relative density, obtain extractum;
(3) getting fructose adds in above-mentioned extractum, and 70 DEG C are uniformly mixed 30 minutes, to dissolving mix homogeneously completely, obtains semi-finished product cream.Semi-finished product cream 70 DEG C heat loaded, 105 DEG C of sterilizings 45 minutes, cooling, product external packaging after loading, makes 1000 bottles, 60g/ bottle altogether.
Usage and dosage: 1 time on the one, each 6g.
Embodiment 2: the preparation of Rhizoma Gastrodiae Herba hylotelephii erythrosticti cream
(1) raw material is taken according to the following ratio: Rhizoma Gastrodiae 220g, Radix Rhodiolae 250g, fructose 145g
(2) Rhizoma Gastrodiae, Radix Rhodiolae raw material powder are broken into coarse powder, cross 40 mesh sieves, mix homogeneously, load extraction pot, add 95% food grade ethanol of raw material 10 times, when being preheating to 45 DEG C, open microwave source, be adjusted to required microwave power, make coarse powder accept microwave radiation in flow process, filter, filtering residue can extract repeatedly; Extract complete, after filtrate reduced in volume, obtain thick product, be 1.25 (60 DEG C of mensuration) by above-mentioned aqueous extract concentrating under reduced pressure (80 DEG C, vacuum is 0.08Mpa) to relative density, obtain extractum;
(3) getting fructose adds in above-mentioned extractum, and 70 DEG C are uniformly mixed 30 minutes, to dissolving mix homogeneously completely, obtains semi-finished product cream.Semi-finished product cream 70 DEG C heat loaded, 105 DEG C of sterilizings 45 minutes, cooling, product external packaging after loading, makes 1000 bottles, 60g/ bottle altogether.
Usage and dosage: 1 time on the one, each 6g.
Embodiment 3: the preparation of Rhizoma Gastrodiae Herba hylotelephii erythrosticti cream
(1) raw material is taken according to the following ratio: Rhizoma Gastrodiae 405g, Radix Rhodiolae 450g, fructose 210g
(2) Rhizoma Gastrodiae, Radix Rhodiolae raw material powder are broken into coarse powder, cross 50 mesh sieves, mix homogeneously, load extraction pot, add 95% food grade ethanol of raw material 12 times, when being preheating to 38 DEG C, open microwave source, be adjusted to required microwave power, make coarse powder accept microwave radiation in flow process, filter, filtering residue can extract repeatedly; Extract complete, after filtrate reduced in volume, obtain thick product, be 1.3 (60 DEG C of mensuration) by above-mentioned aqueous extract concentrating under reduced pressure (70 DEG C, vacuum is 0.08Mpa) to relative density, obtain extractum;
(3) getting fructose adds in above-mentioned extractum, and 65 DEG C are uniformly mixed 30 minutes, to dissolving mix homogeneously completely, obtains semi-finished product cream.Semi-finished product cream 70 DEG C heat loaded, 105 DEG C of sterilizings 45 minutes, cooling, product external packaging after loading, makes 1000 bottles, 60g/ bottle altogether.
Usage and dosage: 1 time on the one, each 6g.
Embodiment 4: the preparation of Rhizoma Gastrodiae Herba hylotelephii erythrosticti cream
(1) raw material is taken according to the following ratio: Rhizoma Gastrodiae 100g, Radix Rhodiolae 450g, fructose 145g
(2) Rhizoma Gastrodiae, Radix Rhodiolae raw material powder are broken into coarse powder, cross 50 mesh sieves, mix homogeneously, load extraction pot, add 95% food grade ethanol of raw material 10 times, when being preheating to 40 DEG C, open microwave source, be adjusted to required microwave power, make coarse powder accept microwave radiation in flow process, filter, filtering residue can extract repeatedly; Extract complete, after filtrate reduced in volume, obtain thick product, be 1.3 (60 DEG C of mensuration) by above-mentioned aqueous extract concentrating under reduced pressure (75 DEG C, vacuum is 0.08Mpa) to relative density, obtain extractum;
(3) getting fructose adds in above-mentioned extractum, and 65 DEG C are uniformly mixed 30 minutes, to dissolving mix homogeneously completely, obtains semi-finished product cream.Semi-finished product cream 70 DEG C heat loaded, 105 DEG C of sterilizings 45 minutes, cooling, product external packaging after loading, makes 1000 bottles, 60g/ bottle altogether.
Usage and dosage: 1 time on the one, each 6g.
Embodiment 5: the preparation of Rhizoma Gastrodiae Herba hylotelephii erythrosticti cream
(1) raw material is taken according to the following ratio: Rhizoma Gastrodiae 405g, Radix Rhodiolae 135g, fructose 145g
(2) Rhizoma Gastrodiae, Radix Rhodiolae raw material powder are broken into coarse powder, cross 40 mesh sieves, mix homogeneously, load extraction pot, add 95% food grade ethanol of raw material 10 times, when being preheating to 35 DEG C, open microwave source, be adjusted to required microwave power, make coarse powder accept microwave radiation in flow process, filter, filtering residue can extract repeatedly; Extract complete, after filtrate reduced in volume, obtain thick product, be 1.3 (60 DEG C of mensuration) by above-mentioned aqueous extract concentrating under reduced pressure (70 DEG C, vacuum is 0.08Mpa) to relative density, obtain extractum;
(3) getting fructose adds in above-mentioned extractum, and 65 DEG C are uniformly mixed 30 minutes, to dissolving mix homogeneously completely, obtains semi-finished product cream.Semi-finished product cream 70 DEG C heat loaded, 105 DEG C of sterilizings 45 minutes, cooling, product external packaging after loading, makes 1000 bottles, 60g/ bottle altogether.
Usage and dosage: 1 time on the one, each 6g.
Embodiment 6: the preparation of Rhizoma Gastrodiae Herba hylotelephii erythrosticti cream
(1) raw material is taken according to the following ratio: Rhizoma Gastrodiae 80g, Radix Rhodiolae 115g, fructose 70g
(2) Rhizoma Gastrodiae, Radix Rhodiolae raw material powder are broken into coarse powder, cross 40 mesh sieves, mix homogeneously, load extraction pot, add 95% food grade ethanol of raw material 8 times, when being preheating to 40 DEG C, open microwave source, be adjusted to required microwave power, make coarse powder accept microwave radiation in flow process, filter, filtering residue can extract repeatedly; Extract complete, after filtrate reduced in volume, obtain thick product, be 1.3 (60 DEG C of mensuration) by above-mentioned aqueous extract concentrating under reduced pressure (75 DEG C, vacuum is 0.08Mpa) to relative density, obtain extractum;
(3) getting fructose adds in above-mentioned extractum, and 70 DEG C are uniformly mixed 30 minutes, to dissolving mix homogeneously completely, obtains semi-finished product cream.Semi-finished product cream 70 DEG C heat loaded, 105 DEG C of sterilizings 45 minutes, cooling, product external packaging after loading, makes 1000 bottles, 60g/ bottle altogether.
Usage and dosage: 1 time on the one, each 6g.
Embodiment 7: the preparation of Rhizoma Gastrodiae Herba hylotelephii erythrosticti cream
(1) raw material is taken according to the following ratio: Rhizoma Gastrodiae 430g, Radix Rhodiolae 480g, fructose 220g
(2) Rhizoma Gastrodiae, Radix Rhodiolae raw material powder are broken into coarse powder, cross 40 mesh sieves, mix homogeneously, load extraction pot, add 95% food grade ethanol of raw material 12 times, when being preheating to 35 DEG C, open microwave source, be adjusted to required microwave power, make coarse powder accept microwave radiation in flow process, filter, filtering residue can extract repeatedly; Extract complete, after filtrate reduced in volume, obtain thick product, be 1.25 (60 DEG C of mensuration) by above-mentioned aqueous extract concentrating under reduced pressure (70 DEG C, vacuum is 0.08Mpa) to relative density, obtain extractum;
(3) getting fructose adds in above-mentioned extractum, and 65 DEG C are uniformly mixed 30 minutes, to dissolving mix homogeneously completely, obtains semi-finished product cream.Semi-finished product cream 70 DEG C heat loaded, 105 DEG C of sterilizings 45 minutes, cooling, product external packaging after loading, makes 1000 bottles, 60g/ bottle altogether.
Usage and dosage: 1 time on the one, each 6g.
Claims (5)
1. a Rhizoma Gastrodiae Herba hylotelephii erythrosticti cream, is characterized in that: calculate according to composition by weight, it is prepared from by following raw material medicaments and adjuvant:
100 ~ 405 parts, Rhizoma Gastrodiae, Radix Rhodiolae 135 ~ 450 parts, 80 ~ 210 parts, fructose.
2. according to Rhizoma Gastrodiae Herba hylotelephii erythrosticti cream according to claim 1, it is characterized in that: calculate according to composition by weight, it is prepared from by following raw material medicaments and adjuvant:
185 ~ 300 parts, Rhizoma Gastrodiae, Radix Rhodiolae 200 ~ 350 parts, 100 ~ 180 parts, fructose.
3. according to Rhizoma Gastrodiae Herba hylotelephii erythrosticti cream according to claim 1, it is characterized in that: calculate according to composition by weight, it is prepared from by following raw material medicaments and adjuvant: 220 parts, Rhizoma Gastrodiae, Radix Rhodiolae 250 parts, 145 parts, fructose.
4. the Rhizoma Gastrodiae Herba hylotelephii erythrosticti cream as described in as arbitrary in claim 1-3, is characterized in that: preparation method is:
(1) Rhizoma Gastrodiae, Radix Rhodiolae raw material powder are broken into coarse powder, cross 20-50 mesh sieve, mix homogeneously, load extraction pot, add raw material 8-12 95% food grade ethanol doubly;
(2) open microwave source when being preheating to about 40 DEG C, be adjusted to required microwave power, make coarse powder accept microwave radiation in flow process;
(3) filter, filtering residue can extract repeatedly; Extract complete, after filtrate reduced in volume, obtain thick product;
(4) by above-mentioned aqueous extract 70-80 DEG C, vacuum be evaporated to 60 DEG C under being 0.08Mpa condition time relative density be 1.20-1.30, obtain extractum;
(5) in 100,000 grades of clean control zones, getting fructose adds in above-mentioned extractum, and 60-70 DEG C is uniformly mixed 30 minutes, to dissolving mix homogeneously completely, obtains semi-finished product cream, semi-finished product cream 60-70 DEG C heat is loaded, specification 60g/ bottle, 105 DEG C of sterilizings 45 minutes, cooling, inspection, to obtain final product.
5. the application of Rhizoma Gastrodiae Herba hylotelephii erythrosticti cream in the medicine preparing enhancing immunity as described in as arbitrary in claim 1-3.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108653585A (en) * | 2018-06-19 | 2018-10-16 | 泸州德高生物科技有限公司 | The preparation method of Rhizoma Gastrodiae paste nourishing agent |
| CN114073682A (en) * | 2021-09-17 | 2022-02-22 | 曲阜师范大学 | Microcapsule decoction piece embedded with components for preventing and improving non-alcoholic fatty liver and preparation method thereof |
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| CN108653585A (en) * | 2018-06-19 | 2018-10-16 | 泸州德高生物科技有限公司 | The preparation method of Rhizoma Gastrodiae paste nourishing agent |
| CN114073682A (en) * | 2021-09-17 | 2022-02-22 | 曲阜师范大学 | Microcapsule decoction piece embedded with components for preventing and improving non-alcoholic fatty liver and preparation method thereof |
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Application publication date: 20160330 |