CN105754000B - Hijiki polysaccharide extraction process - Google Patents
Hijiki polysaccharide extraction process Download PDFInfo
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- CN105754000B CN105754000B CN201610243809.6A CN201610243809A CN105754000B CN 105754000 B CN105754000 B CN 105754000B CN 201610243809 A CN201610243809 A CN 201610243809A CN 105754000 B CN105754000 B CN 105754000B
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- 238000000605 extraction Methods 0.000 title claims abstract description 40
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 26
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 26
- 150000004676 glycans Chemical class 0.000 title claims abstract 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 55
- 239000007788 liquid Substances 0.000 claims abstract description 43
- 239000000284 extract Substances 0.000 claims abstract description 31
- 238000005119 centrifugation Methods 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 238000003756 stirring Methods 0.000 claims abstract description 20
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- 239000000047 product Substances 0.000 claims abstract description 16
- 238000001556 precipitation Methods 0.000 claims abstract description 14
- 239000012141 concentrate Substances 0.000 claims abstract description 13
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- 235000011114 ammonium hydroxide Nutrition 0.000 claims abstract description 7
- 238000001914 filtration Methods 0.000 claims abstract description 7
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 7
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 7
- 239000002994 raw material Substances 0.000 claims abstract description 7
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- 235000020710 ginseng extract Nutrition 0.000 description 1
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- 235000013402 health food Nutrition 0.000 description 1
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- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 235000006479 iodine deficiency Nutrition 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000003907 kidney function Effects 0.000 description 1
- 230000003908 liver function Effects 0.000 description 1
- 231100001252 long-term toxicity Toxicity 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 229960001855 mannitol Drugs 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
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- 235000020232 peanut Nutrition 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
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- 150000003722 vitamin derivatives Chemical class 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
A kind of Hijiki polysaccharide extraction process, 1) 3 tons of water of past No. 1 tank injection；2) 300kg raw materials, stirring extraction are added toward No. 3 tanks；3) extract solution released in No. 3 tanks squeezes into No. 2 tanks；4) enzyme is applied directly in No. 2 tanks, is allowed to dissolve, place 14 hours question responses；5) liquid in No. 2 tanks is squeezed into No. 1 tank, stops when making the liquid concentration in No. 1 tank to 700kg, start to centrifuge；Liquid after centrifugation squeezes into No. 2 tanks, after centrifugation, starts second of concentration, centrifugation；6) concentrate in No. 2 tanks is squeezed into bleacher, adjusts pH to 9 with ammoniacal liquor, add hydrogen peroxide, until becoming yellow；7) after the completion of decolourizing, Alcohol-settling tank is squeezed into, ethanol is added, is stood after stirring；8) precipitation solution of ethanol is filtered, the sediment after filtering adds water, redissolved in de- albumen tank, and volatilize ethanol；9) solution of dissolving filters in centrifuge, and the supernatant of recovery is sent to spray drying post；10) it is spray-dried, the content and protein of product is detected.
Description
Technical field
The present invention relates to technical field of bioengineering, and in particular to a kind of Hijiki polysaccharide extraction process.
Background technology
Hizikia Phaeophyta, dumpling made of glutinous rice flour guiding principle, Fucales, Sargassaceae, Sargassum.Frond yellowish-brown, plump succulence,
It is high 15~40 centimetres, up to more than 2 meters.Frond is made up of rhizoid, stem, blade and air bag.Rhizoid is the base portion set of sucker shape
Device, stem are the major branch of right circular cylinder shape, and blade, air bag, the north is serrated；It is southern then in linear or bar-shaped.Frond is fresh in Huang
Brown, dry product are in black.The variation of leaf is very big, and shape is a lot.It is grown on low tide band rock, is distributed China coast more, North gets
Liaodong Peninsula, south are distributed to the Lezhou Peninsula, and wherein Along Zhejiang Coast is most.Sargassum fusifome is acknowledged as most having edible valency now
The marine algae of value, it is infant and the elderly preferably natural good merchantable brand.Japan is called " longevity greens/mustard green ", every year largely from China
Import.
Contained abundant polysaccharide, eatable cellulose, B family vitamin, alginic acid, mannitol and human body are necessary in sargassum fusifome
Mineral matter and trace element；Its polysaccharide has antitumor action, has the effect of promoting hematopoiesis function and enhancing immunologic function, prevents
Thrombosis, reduce cholesterol and prevent hypertension；Eatable cellulose has obvious benefit to constipation, effectively prevents anal intestine
The generation of disease, there is special efficacy to hypertension, colorectal cancer, anaemia, osteoporosis, diabetes, obesity, artery sclerosis；And
Its contained abundant calcium, iodine, iron, potassium, zinc etc., the even more necessary material of people's physical fitness；The amount of iodine of sargassum fusifome is very high, energy
Prevent the generation of the disease such as Thyroid Gland Swell and children mental retardation, dementia caused by iodine deficiency；Due to the heat contained by sargassum fusifome
It is relatively low, it is this or a kind of fabulous low-heat diet food.
The equal edible sargassum fusifome of population, it is particularly suitable to suffer from goitre, lymphonodi cervicales swollen, edema, tinea pedis, high blood
The disease such as pressure, colorectal cancer, anaemia, osteoporosis, diabetes, obesity, artery sclerosis crowd eats more；Deficiency-cold in spleen and stomach person avoid it is edible,
Sargassum fusifome is cold in nature, sweet-salty；With softening and resolving hard mass, inducing diuresis for removing edema, expel the heat-evil resolving sputum the effect of, for goitre, lymphonodi cervicales
Swollen, edema, tinea pedis etc..
Hijiki polysaccharide has important application value in health products, medicine and cosmetic industry.It mainly applies to resist
The facial mask and face cream skin care item of oxidative function, having prevents Dermatochalasia and wrinkle from producing, and improves UV-induced skin-color
Element precipitation, the desalination effect such as senile plaque expelling and other pigmented spots.
In recent years, the extraction of polysaccharide and purification process have rapid development, and new method also continues to bring out, including HPLC
Method, enzyme process, membrane separation process etc..But due to each side, these methods are not widely used.At present in more uses
Property Hot water extraction or acidleach formulation.Various nutritional ingredients based on Hijiki polysaccharide are largely water soluble substances, because
This can utilize the method for hot water extraction to be extracted；This extracting method simple possible, it is easy to operate, to equipment requirement not
Height, but its production time length, polysaccharide yield is not high, wastes serious.And acidleach formulation then has in destructible polysaccharide with immunocompetence
The specific structure of pass, both of which have its weak point.Also once had been reported that using the method for enzymolysis and extraction polysaccharide, but dwelt in sheep
When dish extracts, cellulase and pectase are added, the institutional framework of sargassum fusifome can be sufficiently destroyed, polysaccharide extract rate can be improved, with
The above two are compared, and have certain advantage.The active ingredient of extract obtained by different process contained by it is different, therefore its
Application field and effect also differ.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of utilization rate height, the controllable Hijiki polysaccharide of product quality
Extraction process.
The technical problems to be solved by the invention are realized using following technical scheme：
A kind of Hijiki polysaccharide extraction process, specific method step are as follows：
(1) 3000kg water is injected toward No. 1 multi-function extractor, temperature regulating is to 90 DEG C；
(2) 300kg sargassum fusifome raw materials being added toward No. 3 multi-function extractors, starts stirring extraction, extraction time is 5 hours,
During, maintain the temperature between 85-90 DEG C, extraction terminates, and samples the content of Detection and Extraction liquid；
(3) extract solution in No. 3 multi-function extractors is released, No. 2 multi-function extractors, temperature dimension are squeezed into after crossing vibratory sieve
Hold at 60-65 DEG C, extracting liq 2200kg, enzyme is weighed by the part by weight of extract solution 8/1000ths；
(4) enzyme is applied directly in No. 2 multi-function extractors, stirring is allowed to dissolve for 30 minutes, is stirred for 3-4 hours, so
14 hours question responses, second day processing enzymolysis liquid, the content of sampling detection enzymolysis liquid after reaction terminates are placed afterwards；
(5) liquid in No. 2 multi-function extractors is squeezed into No. 1 multi-function extractor, two inspissators start simultaneously at concentration,
Stop when making the liquid concentration in No. 1 multi-function extractor to 700kg, start to centrifuge, the sediment of centrifugation is abandoned, sampling detection
Content；
Inspissator is cleaned simultaneously, the liquid after centrifugation squeezes into No. 2 multi-function extractors, starts to centrifuge, and after centrifugation, opens
Begin to concentrate for second, be concentrated into 300kg, then centrifuge once；
(6) concentrate in No. 2 multi-function extractors is squeezed into bleacher, adjusts pH to 9 with ammoniacal liquor, dosage about exists
500ml, temperature maintain 60-65 DEG C, after adding hydrogen peroxide 100kg, 1h, check color, such as unchanged yellow, and it is double to add 40kg
Oxygen water, after 1h, then observation is sampled, and so on, until becoming yellow, about in 150kg；
(7) after the completion of decolourizing, release and weigh weight, squeeze into Alcohol-settling tank, 5-6 times by weight adds 95% ethanol, stirring 20
12h is stood after minute, detects the ethanol content after alcohol precipitation,；
(8) precipitation solution of ethanol is filtered, the sediment after filtering adds 300kg water, redissolved in de- albumen tank, second of volatilizing
Alcohol；
(9) solution of dissolving filters in sedimentation-type centrifuge, removes impurity, and the supernatant of recovery is sent to spray drying hilllock
Position；
(10) solution is spray-dried, and the content and protein of product are detected.
Enzyme described in step (3) is cellulase, hemicellulase, one kind in zytase or combination of two or three kinds
Together, the enzyme activity of three kinds of enzymes is 50,000 U/g.
The addition that enzyme liquid is activated described in step (3) is that substep adds a kind of activation enzyme liquid or added simultaneously two or three
Multiplicity reactivation enzyme liquid.
A kind of nutrient and healthcare products containing above-mentioned Hijiki polysaccharide, are made up of the component of following weight：Hijiki polysaccharide
100g, Herb Gynostemmae Pentaphylli extract 15g, ginseng extract 5g, Astragalus Root P.E 5g, eucommia ulmoides extracts 5g, purple potato leaf extract 5g, oil
Cauliflower extract 5g, white mushroom extract 5g, gumbo extract 4g, peanut leaf extract 5g, lycium ruthenicum extract 5g, chrysanthemum carry
Take thing 5g, cimicifuga foetida extract 5g, Fructus Jujubae extract 5g, Herba Duchesneae Indicae extract 5g.
In order to verify the security of above-mentioned health products, substantial amounts of animal acute toxicity test, Long term Animal toxicity have been carried out
Experiment and a large amount of clinical tests for the treatment of disease, the use link that treatment disease is put into the medicine provide science and objective
Foundation.Its test method and result of the test difference are as follows：
(1) animal acute toxicity test is carried out to health products prepared by the present invention：
Medicine：Bulk drug used in the present invention is commercially available from common Chinese shop, and its specification meets national Chinese medicine mark
It is accurate.
Experimental animal：Small white mouse, body weight are randomly choosed, bought by market in 50g or so, male and female.
Method and result：Small white mouse 30 is taken, it is hungry 20 hours or so, 50g is fed several times daily by every, it is continuous to feed
One week, observe 10 days, mouse activity is normal, non-toxic reaction, no death.Show oral liquid without acute toxicity, clinical application agent
Amount safety.
(2) long-term toxicity test for animals is carried out to health products prepared by the present invention：
Experimental animal：Big white mouse, body weight are randomly choosed, bought by market in 120-130g, male and female.
Method and result：Big white mouse 20 is taken, is divided into 2 groups, every group 10, wherein the 1st group is used as administration group, it is every by every
It feeds 40-50g several times, and the 2nd group of diet as a control group, once a day, is continuously fed 90 days, body weight is surveyed, in most
24 hours sacrificed by decapitation after single administration afterwards, blood, routine urinalysis, liver function, renal function are surveyed, cored, the internal organ such as kidney, tissues observed shape
The change of state, observes the effect of dosage, observation diet, the change of activity, and experiment proves administration group compared with control group without significantly
Change, equal non-toxic reaction, is a kind of safe drugs.
The beneficial effects of the invention are as follows：
1. polyoses content is apparently higher than traditional water body, alcohol-extracting technology in the extract solution made by this technique.
2. the present invention effectively eliminates the fishy smell and bitter taste of simple sargassum fusifome extract solution, the sargassum fusifome extract solution battalion of preparation
Support and enrich, base-material is provided further to prepare sargassum fusifome food.
4. present invention process operating unit is apparent, required equipment is simple, suitable for industrialized production.
Embodiment
In order that the technical means, the inventive features, the objects and the advantages of the present invention are easy to understand, tie below
Specific embodiment is closed, the present invention is expanded on further.
Embodiment 1
A kind of Hijiki polysaccharide extraction process, specific method step are as follows：
(1) 3000kg water is injected toward No. 1 multi-function extractor, temperature regulating is to 90 DEG C；
(2) 300kg sargassum fusifome raw materials being added toward No. 3 multi-function extractors, starts stirring extraction, extraction time is 5 hours,
During, maintain the temperature between 85-90 DEG C, extraction terminates, and samples the content of Detection and Extraction liquid；
(3) extract solution in No. 3 multi-function extractors is released, No. 2 multi-function extractors, temperature dimension are squeezed into after crossing vibratory sieve
Hold at 60-65 DEG C, extracting liq 2200kg, cellulase is weighed by the part by weight of extract solution 8/1000ths；
(4) cellulase is applied directly in No. 2 multi-function extractors, stirring is allowed to dissolve for 30 minutes, and it is small to be stirred for 3-4
When, then place 14 hours question responses, second day processing enzymolysis liquid, the content of sampling detection enzymolysis liquid after reaction terminates；
(5) liquid in No. 2 multi-function extractors is squeezed into No. 1 multi-function extractor, two inspissators start simultaneously at concentration,
Stop when making the liquid concentration in No. 1 multi-function extractor to 700kg, start to centrifuge, the sediment of centrifugation is abandoned, sampling detection
Content；
Inspissator is cleaned simultaneously, the liquid after centrifugation squeezes into No. 2 multi-function extractors, starts to centrifuge, and after centrifugation, opens
Begin to concentrate for second, be concentrated into 300kg, then centrifuge once；
(6) concentrate in No. 2 multi-function extractors is squeezed into bleacher, adjusts pH to 9 with ammoniacal liquor, dosage about exists
500ml, temperature maintain 60-65 DEG C, after adding hydrogen peroxide 100kg, 1h, check color, such as unchanged yellow, and it is double to add 40kg
Oxygen water, after 1h, then observation is sampled, and so on, until becoming yellow, about in 150kg；
(7) after the completion of decolourizing, release and weigh weight, squeeze into Alcohol-settling tank, 5-6 times by weight adds 95% ethanol, stirring 20
12h is stood after minute, detects the ethanol content after alcohol precipitation,；
(8) precipitation solution of ethanol is filtered, the sediment after filtering adds 300kg water, redissolved in de- albumen tank, second of volatilizing
Alcohol；
(9) solution of dissolving filters in sedimentation-type centrifuge, removes impurity, and the supernatant of recovery is sent to spray drying hilllock
Position；
(10) solution is spray-dried, and the content and protein of product are detected.
Implement 2
A kind of Hijiki polysaccharide extraction process, specific method step are as follows：
(1) 3000kg water is injected toward No. 1 multi-function extractor, temperature regulating is to 90 DEG C；
(2) 300kg sargassum fusifome raw materials being added toward No. 3 multi-function extractors, starts stirring extraction, extraction time is 5 hours,
During, maintain the temperature between 85-90 DEG C, extraction terminates, and samples the content of Detection and Extraction liquid；
(3) extract solution in No. 3 multi-function extractors is released, No. 2 multi-function extractors, temperature dimension are squeezed into after crossing vibratory sieve
Hold at 60-65 DEG C, extracting liq 2200kg, hemicellulase is weighed by the part by weight of extract solution 8/1000ths；
(4) hemicellulase is applied directly in No. 2 multi-function extractors, stirring is allowed to dissolve for 30 minutes, is stirred for 3-4
Hour, then place 14 hours question responses, second day processing enzymolysis liquid, the content of sampling detection enzymolysis liquid after reaction terminates；
(5) liquid in No. 2 multi-function extractors is squeezed into No. 1 multi-function extractor, two inspissators start simultaneously at concentration,
Stop when making the liquid concentration in No. 1 multi-function extractor to 700kg, start to centrifuge, the sediment of centrifugation is abandoned, sampling detection
Content；
Inspissator is cleaned simultaneously, the liquid after centrifugation squeezes into No. 2 multi-function extractors, starts to centrifuge, and after centrifugation, opens
Begin to concentrate for second, be concentrated into 300kg, then centrifuge once；
(6) concentrate in No. 2 multi-function extractors is squeezed into bleacher, adjusts pH to 9 with ammoniacal liquor, dosage about exists
500ml, temperature maintain 60-65 DEG C, after adding hydrogen peroxide 100kg, 1h, check color, such as unchanged yellow, and it is double to add 40kg
Oxygen water, after 1h, then observation is sampled, and so on, until becoming yellow, about in 150kg；
(7) after the completion of decolourizing, release and weigh weight, squeeze into Alcohol-settling tank, 5-6 times by weight adds 95% ethanol, stirring 20
12h is stood after minute, detects the ethanol content after alcohol precipitation,；
(8) precipitation solution of ethanol is filtered, the sediment after filtering adds 300kg water, redissolved in de- albumen tank, second of volatilizing
Alcohol；
(9) solution of dissolving filters in sedimentation-type centrifuge, removes impurity, and the supernatant of recovery is sent to spray drying hilllock
Position；
(10) solution is spray-dried, and the content and protein of product are detected.
Embodiment 3
A kind of Hijiki polysaccharide extraction process, specific method step are as follows：
(1) 3000kg water is injected toward No. 1 multi-function extractor, temperature regulating is to 90 DEG C；
(2) 300kg sargassum fusifome raw materials being added toward No. 3 multi-function extractors, starts stirring extraction, extraction time is 5 hours,
During, maintain the temperature between 85-90 DEG C, extraction terminates, and samples the content of Detection and Extraction liquid；
(3) extract solution in No. 3 multi-function extractors is released, No. 2 multi-function extractors, temperature dimension are squeezed into after crossing vibratory sieve
Hold at 60-65 DEG C, extracting liq 2200kg, zytase is weighed by the part by weight of extract solution 8/1000ths；
(4) zytase is applied directly in No. 2 multi-function extractors, stirring is allowed to dissolve for 30 minutes, and it is small to be stirred for 3-4
When, then place 14 hours question responses, second day processing enzymolysis liquid, the content of sampling detection enzymolysis liquid after reaction terminates；
(5) liquid in No. 2 multi-function extractors is squeezed into No. 1 multi-function extractor, two inspissators start simultaneously at concentration,
Stop when making the liquid concentration in No. 1 multi-function extractor to 700kg, start to centrifuge, the sediment of centrifugation is abandoned, sampling detection
Content；
Inspissator is cleaned simultaneously, the liquid after centrifugation squeezes into No. 2 multi-function extractors, starts to centrifuge, and after centrifugation, opens
Begin to concentrate for second, be concentrated into 300kg, then centrifuge once；
(6) concentrate in No. 2 multi-function extractors is squeezed into bleacher, adjusts pH to 9 with ammoniacal liquor, dosage about exists
500ml, temperature maintain 60-65 DEG C, after adding hydrogen peroxide 100kg, 1h, check color, such as unchanged yellow, and it is double to add 40kg
Oxygen water, after 1h, then observation is sampled, and so on, until becoming yellow, about in 150kg；
(7) after the completion of decolourizing, release and weigh weight, squeeze into Alcohol-settling tank, 5-6 times by weight adds 95% ethanol, stirring 20
12h is stood after minute, detects the ethanol content after alcohol precipitation,；
(8) precipitation solution of ethanol is filtered, the sediment after filtering adds 300kg water, redissolved in de- albumen tank, second of volatilizing
Alcohol；
(9) solution of dissolving filters in sedimentation-type centrifuge, removes impurity, and the supernatant of recovery is sent to spray drying hilllock
Position；
(10) solution is spray-dried, and the content and protein of product are detected.
Embodiment 4
A kind of Hijiki polysaccharide extraction process, specific method step are as follows：
(1) 3000kg water is injected toward No. 1 multi-function extractor, temperature regulating is to 90 DEG C；
(2) 300kg sargassum fusifome raw materials being added toward No. 3 multi-function extractors, starts stirring extraction, extraction time is 5 hours,
During, maintain the temperature between 85-90 DEG C, extraction terminates, and samples the content of Detection and Extraction liquid；
(3) extract solution in No. 3 multi-function extractors is released, No. 2 multi-function extractors, temperature dimension are squeezed into after crossing vibratory sieve
Hold at 60-65 DEG C, extracting liq 2200kg, hemicellulase and zytase are weighed by the part by weight of extract solution 8/1000ths
Mixed enzyme；
(4) hemicellulase and zytase are applied directly in No. 2 multi-function extractors, stirring is allowed to molten in 30 minutes
Solution, is stirred for 3-4 hours, then places 14 hours question responses, second day processing enzymolysis liquid, sampling detection enzymolysis after reaction terminates
The content of liquid；
(5) liquid in No. 2 multi-function extractors is squeezed into No. 1 multi-function extractor, two inspissators start simultaneously at concentration,
Stop when making the liquid concentration in No. 1 multi-function extractor to 700kg, start to centrifuge, the sediment of centrifugation is abandoned, sampling detection
Content；
Inspissator is cleaned simultaneously, the liquid after centrifugation squeezes into No. 2 multi-function extractors, starts to centrifuge, and after centrifugation, opens
Begin to concentrate for second, be concentrated into 300kg, then centrifuge once；
(6) concentrate in No. 2 multi-function extractors is squeezed into bleacher, adjusts pH to 9 with ammoniacal liquor, dosage about exists
500ml, temperature maintain 60-65 DEG C, after adding hydrogen peroxide 100kg, 1h, check color, such as unchanged yellow, and it is double to add 40kg
Oxygen water, after 1h, then observation is sampled, and so on, until becoming yellow, about in 150kg；
(7) after the completion of decolourizing, release and weigh weight, squeeze into Alcohol-settling tank, 5-6 times by weight adds 95% ethanol, stirring 20
12h is stood after minute, detects the ethanol content after alcohol precipitation,；
(8) precipitation solution of ethanol is filtered, the sediment after filtering adds 300kg water, redissolved in de- albumen tank, second of volatilizing
Alcohol；
(9) solution of dissolving filters in sedimentation-type centrifuge, removes impurity, and the supernatant of recovery is sent to spray drying hilllock
Position；
(10) solution is spray-dried, and the content and protein of product are detected.
Detection method according to polysaccharide in the health food inside health products specification is detected, and by the above-mentioned work of the present invention
Compared with skill is extracted with single enzyme, as a result it see the table below, the results showed that enzymolysis and extraction effect of the present invention is good, polysaccharide extract rate
It is high.
The general principle and principal character and advantages of the present invention of the present invention has been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the simply explanation described in above-described embodiment and specification is originally
The principle of invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (2)
1. a kind of Hijiki polysaccharide extraction process, it is characterised in that specific method step is as follows：
(1) 3000kg water is injected toward No. 1 multi-function extractor, temperature regulating is to 90 DEG C；
(2) 300kg sargassum fusifome raw materials are added toward No. 3 multi-function extractors, starts stirring extraction, extraction time is 5 hours, process
In, maintain the temperature between 85-90 DEG C, extraction terminates, and samples the content of Detection and Extraction liquid；
(3) extract solution in No. 3 multi-function extractors is released, squeezes into No. 2 multi-function extractors after crossing vibratory sieve, temperature maintains
60-65 DEG C, extracting liq 2200kg, enzyme is weighed by the part by weight of extract solution 8/1000ths；
(4) enzyme is applied directly in No. 2 multi-function extractors, stirring is allowed to dissolve for 30 minutes, is stirred for 3-4 hours, Ran Houfang
Put 14 hours question responses, second day processing enzymolysis liquid, the content of sampling detection enzymolysis liquid after reaction terminates；
(5) liquid in No. 2 multi-function extractors is squeezed into No. 1 multi-function extractor, two inspissators start simultaneously at concentration, make 1
Stop when liquid concentration in number multi-function extractor is to 700kg, start to centrifuge, the sediment of centrifugation is abandoned, and sampling detection contains
Amount；Liquid after centrifugation squeezes into No. 2 multi-function extractors, starts to centrifuge, and after centrifugation, starts second and concentrates, be concentrated into
300kg, then centrifuge once；
(6) concentrate in No. 2 multi-function extractors is squeezed into bleacher, adjusts pH to 9 with ammoniacal liquor, dosage is about in 500ml, temperature
Degree maintains 60-65 DEG C, adds hydrogen peroxide 100kg, after 1 hour, checks color, such as unchanged yellow, adds 40kg hydrogen peroxide,
After 1 hour, then observation is sampled, and so on, until becoming yellow；
(7) after the completion of decolourizing, release and weigh weight, squeeze into Alcohol-settling tank, 5-6 times by weight adds 95% ethanol, stirs 20 minutes
12 hours are stood afterwards, detects the ethanol content after alcohol precipitation；
(8) precipitation solution of ethanol is filtered, the sediment after filtering adds 300kg water, redissolved in de- albumen tank, and volatilize ethanol；
(9) solution of dissolving filters in sedimentation-type centrifuge, removes impurity, and the supernatant of recovery is sent to spray drying post；
(10) solution is spray-dried, and obtains Hijiki polysaccharide product, and the content and protein of product are detected.
2. Hijiki polysaccharide extraction process according to claim 1, it is characterised in that enzyme described in step (3) is fiber
Together, the enzyme activity of three kinds of enzymes is 50,000 U/g for one kind or combination of two in plain enzyme, hemicellulase, zytase or three kinds.
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| CN109369819A (en) * | 2018-10-22 | 2019-02-22 | 温州科技职业学院 | A kind of extracting method of Hijiki polysaccharide |
| CN110396138B (en) | 2019-08-29 | 2021-02-19 | 华南理工大学 | Method for decoloring and deproteinizing brown algae polysaccharide |
| CN114107410A (en) * | 2021-12-02 | 2022-03-01 | 安徽坤大生物工程技术有限公司 | Method for extracting and fermenting sargassum fusiforme polysaccharide |
| CN115363208A (en) * | 2022-06-01 | 2022-11-22 | 广东省农业科学院蚕业与农产品加工研究所 | Preparation method of sargassum fusiforme aqueous extract for treating constipation |
| CN115537437A (en) * | 2022-09-22 | 2022-12-30 | 青岛农业大学 | Low-molecular-weight sargassum fusiforme enzymolysis product extract, and preparation method and application thereof |
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| WO2005016028A1 (en) * | 2003-08-18 | 2005-02-24 | Sealight Corporation Company | Method for manufacturing a biological imitation rice mixed with polysaccharide from brown seaweed and a polysaccharide paste from brown seaweed |
| CN1962699A (en) * | 2006-11-25 | 2007-05-16 | 中国烟草总公司郑州烟草研究院 | Hijiki polysaccharide and its uses in tobacco |
| CN101250232A (en) * | 2008-03-27 | 2008-08-27 | 钱国英 | Extraction technique of sargassum fusiform active polysaccharides |
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|---|---|---|---|---|
| WO2005016028A1 (en) * | 2003-08-18 | 2005-02-24 | Sealight Corporation Company | Method for manufacturing a biological imitation rice mixed with polysaccharide from brown seaweed and a polysaccharide paste from brown seaweed |
| CN1962699A (en) * | 2006-11-25 | 2007-05-16 | 中国烟草总公司郑州烟草研究院 | Hijiki polysaccharide and its uses in tobacco |
| CN101250232A (en) * | 2008-03-27 | 2008-08-27 | 钱国英 | Extraction technique of sargassum fusiform active polysaccharides |
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