CN114107410A - Method for extracting and fermenting sargassum fusiforme polysaccharide - Google Patents
Method for extracting and fermenting sargassum fusiforme polysaccharide Download PDFInfo
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- CN114107410A CN114107410A CN202111465813.4A CN202111465813A CN114107410A CN 114107410 A CN114107410 A CN 114107410A CN 202111465813 A CN202111465813 A CN 202111465813A CN 114107410 A CN114107410 A CN 114107410A
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- 241000264279 Sargassum fusiforme Species 0.000 title claims abstract description 45
- 150000004676 glycans Chemical class 0.000 title claims abstract description 34
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 34
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 32
- 238000000855 fermentation Methods 0.000 claims abstract description 36
- 230000004151 fermentation Effects 0.000 claims abstract description 36
- 239000000706 filtrate Substances 0.000 claims abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 18
- 230000001954 sterilising effect Effects 0.000 claims abstract description 18
- 238000001914 filtration Methods 0.000 claims abstract description 15
- 229910001385 heavy metal Inorganic materials 0.000 claims abstract description 15
- 239000012535 impurity Substances 0.000 claims abstract description 12
- 230000007246 mechanism Effects 0.000 claims abstract description 12
- 239000002244 precipitate Substances 0.000 claims abstract description 12
- 238000004537 pulping Methods 0.000 claims abstract description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 12
- 238000001035 drying Methods 0.000 claims abstract description 11
- 238000000926 separation method Methods 0.000 claims abstract description 10
- 239000008213 purified water Substances 0.000 claims abstract description 9
- 239000002002 slurry Substances 0.000 claims abstract description 9
- 238000001179 sorption measurement Methods 0.000 claims abstract description 9
- 238000003756 stirring Methods 0.000 claims abstract description 9
- 239000000047 product Substances 0.000 claims abstract description 8
- 238000001291 vacuum drying Methods 0.000 claims abstract description 7
- 238000005406 washing Methods 0.000 claims abstract description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000004140 cleaning Methods 0.000 claims abstract description 6
- 238000007710 freezing Methods 0.000 claims abstract description 6
- 230000008014 freezing Effects 0.000 claims abstract description 6
- 238000000227 grinding Methods 0.000 claims abstract description 6
- 239000001963 growth medium Substances 0.000 claims abstract description 6
- 230000001376 precipitating effect Effects 0.000 claims abstract description 6
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 4
- 240000006439 Aspergillus oryzae Species 0.000 claims description 3
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims description 3
- 244000063299 Bacillus subtilis Species 0.000 claims description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 3
- 241000186000 Bifidobacterium Species 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 3
- 238000000605 extraction Methods 0.000 abstract description 8
- 230000000694 effects Effects 0.000 description 4
- 238000002386 leaching Methods 0.000 description 3
- 241001474374 Blennius Species 0.000 description 2
- 241001466452 Laminariaceae Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 208000037259 Amyloid Plaque Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 208000012641 Pigmentation disease Diseases 0.000 description 1
- 241000195475 Sargassaceae Species 0.000 description 1
- 241000195474 Sargassum Species 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000036782 biological activation Effects 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 230000001815 facial effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000005965 immune activity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Genetics & Genomics (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention discloses an extraction and fermentation method of sargassum fusiforme polysaccharide, which comprises the following steps: s1: cleaning: removing diseased residual roots and fibrous roots, and washing with clear water to remove residual impurities on the surface of the sargassum fusiforme; s2: crushing and pulping: crushing and grinding the cleaned sargassum fusiforme; adding purified water for pulping; s3: fermentation: adding a fermentation culture medium into the slurry obtained in the step S2, and adding a fermentation strain for fermentation; s4: and (3) filtering: filtering the fermentation liquor to remove impurities to obtain filtrate; s5: removing heavy metals: removing heavy metals in the filtrate by an activated carbon adsorption mechanism; s6: and (3) sterilization: sterilizing the filtrate obtained in step S5; s7: concentrating and precipitating: adding absolute ethyl alcohol into the filtrate, stirring, standing for a period of time, performing centrifugal separation, and collecting precipitate; s8: and (3) vacuum drying: and (5) freezing and drying in vacuum to obtain the polysaccharide product. The invention is convenient for extracting the polysaccharide, and can improve the extraction efficiency and the purity of the polysaccharide.
Description
Technical Field
The invention relates to the technical field of sargassum fusiforme polysaccharide extraction, in particular to a sargassum fusiforme polysaccharide extraction and fermentation method.
Background
Sargassum fusiforme is a perennial warm-temperate seaweed belonging to the genus Sargassum of the family Sargassaceae, the family Laminariaceae, and the family Laminariaceae. Mainly distributed in the Liaodong peninsula in North and the peninsula in south to Rezhou, which are the most distributed in the Zhejiang coastal areas. A large number of data researches show that the sargassum fusiforme polysaccharide extracted from the seaweed has good anticancer and hypertension effects besides being widely applied to food and health care industries, and also has good skin care effects by being added as a cosmetic raw material, can relieve skin thirst, recover water balance, and achieve the effects of moisturizing, strengthening and firming skin through biological activation. The sargassum fusiforme polysaccharide has important application value in the industries of health care products, medicines and cosmetics. It is mainly used in antioxidant facial mask and cream skin care product, and has effects of preventing skin relaxation and wrinkle, improving skin pigmentation caused by ultraviolet, and removing senile plaque and other pigmented spots.
In recent years, methods for extracting and purifying polysaccharides have been rapidly developed, and new methods including HPLC methods, enzymatic methods, membrane separation methods, and the like have been increasingly developed. However, for various reasons, these methods have not been widely used. At present, a neutral hot water leaching method or an acid leaching method is mostly adopted. Most of various nutrient components mainly containing sargassum fusiforme polysaccharide are water-soluble substances, so that the sargassum fusiforme polysaccharide can be extracted by a hot water extraction method; the extraction method is simple and feasible, convenient to operate and low in equipment requirement, but the production time is long, the polysaccharide yield is not high, and the waste is serious. Acid leaching tends to destroy specific structures in the polysaccharide that are associated with immune activity, and both methods have their disadvantages.
Disclosure of Invention
The invention aims to provide an extraction and fermentation method of sargassum fusiforme polysaccharide, which aims to solve the problems in the prior art.
In order to achieve the purpose, the invention provides the following technical scheme: a method for extracting and fermenting sargassum fusiforme polysaccharide comprises the following steps:
s1: cleaning: removing diseased residual roots and fibrous roots, and washing with clear water to remove residual impurities on the surface of the sargassum fusiforme;
s2: crushing and pulping: crushing and grinding the cleaned sargassum fusiforme; adding purified water for pulping;
s3: fermentation: adding a fermentation culture medium into the slurry obtained in the step S2, and adding a fermentation strain for fermentation;
s4: and (3) filtering: filtering the fermentation liquor to remove impurities to obtain filtrate;
s5: removing heavy metals: removing heavy metals in the filtrate by an activated carbon adsorption mechanism;
s6: and (3) sterilization: sterilizing the filtrate obtained in step S5;
s7: concentrating and precipitating: adding absolute ethyl alcohol into the filtrate, stirring, standing for a period of time, performing centrifugal separation, and collecting precipitate;
s8: and (3) vacuum drying: and (5) freezing and drying in vacuum to obtain the polysaccharide product.
Preferably, the step S1 further includes drying the washed sargassum fusiforme, and drying the water of the washed sargassum fusiforme.
Preferably, the Sargassum fusiforme crushed in the step S2 needs to be screened by a screen of 40-100 meshes; the proportion of sargassum fusiforme and purified water is 1: 2-1: 4 in the middle.
Preferably, the fermentation in step S3 is to add complex enzyme liquid into the slurry for preliminary enzymolysis; after enzymolysis, adding aspergillus oryzae, bifidobacterium and bacillus subtilis to stir and ferment; finally, the compound enzyme solution is added again for enzymolysis again.
Preferably, the mesh number of the filter used for the filtering in step S4 needs to be less than 100 meshes.
Preferably, the step S4 further includes centrifuging the filtrate to remove a precipitate formed by centrifugation.
Preferably, the step S5 of removing heavy metals is to pass the filtrate through an activated carbon adsorption mechanism.
Preferably, the sterilization in step S6 is performed by high temperature sterilization.
Preferably, the rotation speed of the centrifugal separation in the step S7 is 2500-; the precipitate was collected by a filter mechanism having a pore size of 0.45 μm.
Compared with the prior art, the invention has the beneficial effects that: s1: cleaning: removing diseased residual roots and fibrous roots, and washing with clear water to remove residual impurities on the surface of the sargassum fusiforme; s2: crushing and pulping: crushing and grinding the cleaned sargassum fusiforme; adding purified water for pulping; s3: fermentation: adding a fermentation culture medium into the slurry obtained in the step S2, and adding a fermentation strain for fermentation; s4: and (3) filtering: filtering the fermentation liquor to remove impurities to obtain filtrate; s5: removing heavy metals: removing heavy metals in the filtrate by an activated carbon adsorption mechanism; s6: and (3) sterilization: sterilizing the filtrate obtained in step S5; s7: concentrating and precipitating: adding absolute ethyl alcohol into the filtrate, stirring, standing for a period of time, performing centrifugal separation, and collecting precipitate; s8: and (3) vacuum drying: freezing and vacuum drying to obtain polysaccharide product; the extraction of polysaccharide is convenient for this application, and can improve extraction efficiency and the purity of polysaccharide.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
fig. 1 is a schematic structural view of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Referring to fig. 1, in an embodiment of the present invention, a method for extracting and fermenting sargassum fusiforme polysaccharide includes the following steps:
s1: cleaning: removing diseased residual roots and fibrous roots, and washing with clear water to remove residual impurities on the surface of the sargassum fusiforme;
s2: crushing and pulping: crushing and grinding the cleaned sargassum fusiforme; adding purified water for pulping;
s3: fermentation: adding a fermentation culture medium into the slurry obtained in the step S2, and adding a fermentation strain for fermentation;
s4: and (3) filtering: filtering the fermentation liquor to remove impurities to obtain filtrate;
s5: removing heavy metals: removing heavy metals in the filtrate by an activated carbon adsorption mechanism;
s6: and (3) sterilization: sterilizing the filtrate obtained in step S5;
s7: concentrating and precipitating: adding absolute ethyl alcohol into the filtrate, stirring, standing for a period of time, performing centrifugal separation, and collecting precipitate;
s8: and (3) vacuum drying: and (5) freezing and drying in vacuum to obtain the polysaccharide product.
Preferably, the step S1 further includes drying the washed sargassum fusiforme, and drying the water of the washed sargassum fusiforme.
Preferably, the Sargassum fusiforme crushed in the step S2 needs to be screened by a screen of 40-100 meshes; the proportion of sargassum fusiforme and purified water is 1: 2-1: 4 in the middle.
Preferably, the fermentation in step S3 is to add complex enzyme liquid into the slurry for preliminary enzymolysis; after enzymolysis, adding aspergillus oryzae, bifidobacterium and bacillus subtilis to stir and ferment; finally, the compound enzyme solution is added again for enzymolysis again.
Preferably, the mesh number of the filter used for the filtering in step S4 needs to be less than 100 meshes.
Preferably, the step S4 further includes centrifuging the filtrate to remove a precipitate formed by centrifugation.
Preferably, the step S5 of removing heavy metals is to pass the filtrate through an activated carbon adsorption mechanism.
Preferably, the sterilization in step S6 is performed by high temperature sterilization.
Preferably, the rotation speed of the centrifugal separation in the step S7 is 2500-; the precipitate was collected by a filter mechanism having a pore size of 0.45 μm.
The working principle of the invention is as follows: s1: cleaning: removing diseased residual roots and fibrous roots, and washing with clear water to remove residual impurities on the surface of the sargassum fusiforme; s2: crushing and pulping: crushing and grinding the cleaned sargassum fusiforme; adding purified water for pulping; s3: fermentation: adding a fermentation culture medium into the slurry obtained in the step S2, and adding a fermentation strain for fermentation; s4: and (3) filtering: filtering the fermentation liquor to remove impurities to obtain filtrate; s5: removing heavy metals: removing heavy metals in the filtrate by an activated carbon adsorption mechanism; s6: and (3) sterilization: sterilizing the filtrate obtained in step S5; s7: concentrating and precipitating: adding absolute ethyl alcohol into the filtrate, stirring, standing for a period of time, performing centrifugal separation, and collecting precipitate; s8: and (3) vacuum drying: and (5) freezing and drying in vacuum to obtain the polysaccharide product.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. A method for extracting and fermenting sargassum fusiforme polysaccharide is characterized by comprising the following steps: the method comprises the following steps:
s1: cleaning: removing diseased residual roots and fibrous roots, and washing with clear water to remove residual impurities on the surface of the sargassum fusiforme;
s2: crushing and pulping: crushing and grinding the cleaned sargassum fusiforme; adding purified water for pulping;
s3: fermentation: adding a fermentation culture medium into the slurry obtained in the step S2, and adding a fermentation strain for fermentation;
s4: and (3) filtering: filtering the fermentation liquor to remove impurities to obtain filtrate;
s5: removing heavy metals: removing heavy metals in the filtrate by an activated carbon adsorption mechanism;
s6: and (3) sterilization: sterilizing the filtrate obtained in step S5;
s7: concentrating and precipitating: adding absolute ethyl alcohol into the filtrate, stirring, standing for a period of time, performing centrifugal separation, and collecting precipitate;
s8: and (3) vacuum drying: and (5) freezing and drying in vacuum to obtain the polysaccharide product.
2. The method for extracting and fermenting sargassum fusiforme polysaccharide as claimed in claim 1, wherein the method comprises the following steps: the step S1 further includes drying after washing, and drying the water of the washed sargassum fusiforme.
3. The method for extracting and fermenting sargassum fusiforme polysaccharide as claimed in claim 1, wherein the method comprises the following steps: in the step S2, the sargassum fusiforme is crushed and then screened by a screen of 40-100 meshes; the proportion of sargassum fusiforme and purified water is 1: 2-1: 4 in the middle.
4. The method for extracting and fermenting sargassum fusiforme polysaccharide as claimed in claim 1, wherein the method comprises the following steps: the fermentation in the step S3 is to add compound enzyme liquid into the slurry for enzymolysis in advance; after enzymolysis, adding aspergillus oryzae, bifidobacterium and bacillus subtilis to stir and ferment; finally, the compound enzyme solution is added again for enzymolysis again.
5. The method for extracting and fermenting sargassum fusiforme polysaccharide as claimed in claim 1, wherein the method comprises the following steps: the mesh number of the filter used for the filtering in the step S4 needs to be less than 100 meshes.
6. The method for extracting and fermenting sargassum fusiforme polysaccharide as claimed in claim 1 or 5, wherein: the step S4 further includes centrifuging the filtrate to remove precipitates formed by centrifugation.
7. The method for extracting and fermenting sargassum fusiforme polysaccharide as claimed in claim 1, wherein the method comprises the following steps: in the step S5, the removing of the heavy metals is to pass the filtrate through an activated carbon adsorption mechanism.
8. The method for extracting and fermenting sargassum fusiforme polysaccharide as claimed in claim 1, wherein the method comprises the following steps: the sterilization in the step S6 is performed by high temperature sterilization.
9. The method for extracting and fermenting sargassum fusiforme polysaccharide as claimed in claim 1, wherein the method comprises the following steps: the rotation speed of the centrifugal separation in the step S7 is 2500-3000r/min, and the processing time is 15-20 min; the precipitate was collected by a filter mechanism having a pore size of 0.45 μm.
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Cited By (1)
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CN114621980A (en) * | 2022-03-10 | 2022-06-14 | 温州大学 | Method for improving hypoglycemic activity of sargassum fusiforme extract |
Citations (3)
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CN105754000A (en) * | 2016-04-18 | 2016-07-13 | 安徽坤大生物工程技术有限公司 | Hizikia fusiforme polysaccharide extracting technology |
CN109097403A (en) * | 2018-08-10 | 2018-12-28 | 青岛海莱美生物科技有限公司 | A kind of preparation method of brown alga extract |
CN109369819A (en) * | 2018-10-22 | 2019-02-22 | 温州科技职业学院 | A kind of extracting method of Hijiki polysaccharide |
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105754000A (en) * | 2016-04-18 | 2016-07-13 | 安徽坤大生物工程技术有限公司 | Hizikia fusiforme polysaccharide extracting technology |
CN109097403A (en) * | 2018-08-10 | 2018-12-28 | 青岛海莱美生物科技有限公司 | A kind of preparation method of brown alga extract |
CN109369819A (en) * | 2018-10-22 | 2019-02-22 | 温州科技职业学院 | A kind of extracting method of Hijiki polysaccharide |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN114621980A (en) * | 2022-03-10 | 2022-06-14 | 温州大学 | Method for improving hypoglycemic activity of sargassum fusiforme extract |
CN114621980B (en) * | 2022-03-10 | 2023-04-25 | 温州大学 | Method for improving hypoglycemic activity of sargassum fusiforme extract |
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