CN108239177A - A kind of method that purifying prepares high-quality biological polysaccharide - Google Patents
A kind of method that purifying prepares high-quality biological polysaccharide Download PDFInfo
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- CN108239177A CN108239177A CN201611215234.3A CN201611215234A CN108239177A CN 108239177 A CN108239177 A CN 108239177A CN 201611215234 A CN201611215234 A CN 201611215234A CN 108239177 A CN108239177 A CN 108239177A
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
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- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
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Abstract
The invention discloses a kind of methods that purifying prepares high-quality biological polysaccharide.The preparation method of beta glucan provided by the invention includes the following steps：(1) schizophyllary fermentating liquid with water is mixed and carries out digestion, obtain digestion liquid；(2) separation of solid and liquid is carried out to step (1) the digestion liquid, takes clear liquid；(3) activated carbon is added in the clear liquid obtained in step (2), adsorption treatment is carried out, obtains containing carbon mixture；Separation of solid and liquid is carried out containing carbon mixture to described, takes clear liquid；(4) clear liquid for obtaining step (3) is mixed with ethyl alcohol carries out alcohol analysis, collects the solid of precipitation；(5) solid described in step (4) is dried, you can obtain the beta glucan.Enzymolysis process is not used in the method for the present invention, and cost is relatively low.Acidolysis or alkaline hydrolysis technique is not used, the functional structure of beta glucan will not be damaged.Beta glucan solution viscosity obtained is big, and light transmittance is high, suitable for various applications.
Description
Technical field
The present invention relates to the preparation methods of beta glucan, and in particular to a kind of purifying prepares the side of high-quality biological polysaccharide
Method.
Background technology
Polysaccharide refers to a kind of natural polymer being polymerized by 10 or more monosaccharide molecules, is distributed widely in dynamic
In object, plant, microorganism (bacterium and fungi) and algae lichens.Polysaccharide, which has to adjust as important bioactive substance, exempts from
Epidemic disease, antitumor, reduction glycolipid, anti-aging and other effects have wide answer in fields such as health care, food, animal-breedings
Use prospect.
Beta glucan is a kind of natural polysaccharide.The beta glucan of suitable multiple types can be found in natural environment, usually
It is present in the cell wall of the bacterium of Special Category, saccharomycete, fungi (ganoderma lucidum), also is present in the coating of higher plant seed
In.Mushroom glucan wherein using β -1,3- glycosidic bonds as skeleton has the work(such as strengthen immunity, antitumor, antibacterial, antiviral
Can, in moisturizing, crease-resistant, anti-oxidant, uvioresistant, promote wound healing etc. that also there is remarkable result, in addition, with traditional ferment
Female or cereal glucan is compared, and mushroom glucan has the structure of more stable homogeneous, higher molecular mass and more excellent
It is water-soluble.Therefore the mushroom beta glucan solution of high viscosity, high transparency is widely used in food, cosmetics and pharmaceutical field,
Prepare high viscosity, the mushroom beta glucan solution of high transparency has great importance.
At present, there are mainly two types of the extracting methods of beta glucan, one is true from the fructifications such as the cereal such as oat or mushroom
It is directly extracted in bacterium；The second is by the liquid fermentation of fungi or bacterium, via extracting processing to zymotic fluid, with obtain β-
Glucan.Fermentation method prepares beta glucan, and when purifying needs to remove the impurity such as the nucleic acid generated in fermentation process and protein, passes
System method removes nucleic acid and protein using soda acid processing or enzymatic treatment, and the processing of with high costs and soda acid can cause glucan
It destroys.
Invention content
The object of the present invention is to provide a kind of method for purifying and preparing high-quality beta glucan, the method for the present invention operation letters
Just, without through soda acid and enzymolysis processing, preparation-obtained beta glucan solution has high viscosity, high transparency.
A kind of preparation method of beta glucan provided by the invention, includes the following steps：
(1) schizophyllary fermentating liquid and water are mixed and carries out digestion, obtain digestion liquid；
(2) separation of solid and liquid is carried out to step (1) the digestion liquid, takes clear liquid；
(3) activated carbon is added in the clear liquid obtained in step (2), adsorption treatment is carried out, obtains containing carbon mixture；Contain to described
Carbon mixture carries out separation of solid and liquid, takes clear liquid；
(4) clear liquid for obtaining step (3) is mixed with ethyl alcohol carries out alcohol analysis, collects the solid of precipitation；
(5) solid described in step (4) is dried, you can obtain the beta glucan.
In above-mentioned preparation method, in step (1), conventional liq fermentation process system can be used in the schizophyllary fermentating liquid
It is standby, can be common fermentation condition prepare or fermentation condition optimization after the schizophyllum commune (Schizophyllum that is prepared
Commune Fr.) zymotic fluid.It can specifically be made by the steps to obtain：
(a) schizophyllum commune actication of culture：By potato 200g/L, 20~30g/L of glucose, 10~20g/L of sodium chloride, agar
Plating medium is made in 15~20g/L, and schizophyllum commune strain is inoculated on the plating medium, in 20 DEG C~30 DEG C constant temperature incubations
Culture obtains tablet mycelium in 4~10 days in case；
(b) seed activation：By 50~200g/L of potato starch, 20~60g/L of glucose, 1~10g/L of yeast extract,
Fluid nutrient medium made of 1~10g/L of yeast extract, water is packed into shaking flask, and liquid amount is 1/5~1/3, is taken obtained by step (a)
Tablet mycelium is inoculated with the shaking flask, in 100~220rpm shake cultures 4~10 days in 20 DEG C~30 DEG C constant-temperature tables, as kind
Sub- liquid；
(c) fermented and cultured：By 20~80g/L of glucose, 50~100g/L of sucrose, 5~30g/L of analysis for soybean powder, yeast extract 1
~10g/L, 0.1~2g/L of potassium dihydrogen phosphate, 0.1~2g/L of bitter salt, 0.1~2g/L of ammonium sulfate, potassium nitrate 1~
Fermentation medium made of 10g/L, water is added in fermentation tank and is sterilized 15~30 minutes for 115~128 DEG C, then will be obtained by step (b)
Seed liquor be inoculated in above-mentioned fermentation tank, 20 DEG C~30 DEG C 100~600rpm of constant temperature stirring 2~10Lpm aerobic fementations cultures 5~
10 days.
The volume ratio of the schizophyllary fermentating liquid and water can be 1：(0.1~100), concretely 1：(1~10), 1：(1~
5)、1：(1~4), 1：(3~5), 1：(3~4) 1：1、1：3、1：5 or 1：10；The temperature of the digestion can be 30~121 DEG C, tool
Body can be 50~90 DEG C, 50~70 DEG C, 60~90 DEG C, 50 DEG C, 60 DEG C, 70 DEG C or 90 DEG C, and the time can be 0.2~120h, specifically
Can be 0.5~8h, 0.5~4h, 2~8h, 0.5h, 2h, 4h or 8h.
In above-mentioned preparation method, in step (2), the method that the separation of solid and liquid can use can be centrifugation, just press through
Any one of filter and at least one of negative pressure filtration, concretely following 1) -3)：1) in 500~13000rpm (such as
1~120min (such as 5min) is centrifuged under 4000rpm)；2) negative pressure leaching is carried out with the filter cloth of 100~1500 mesh (such as 300 mesh)；3)
3~60min (such as 5min) is first centrifuged under 500~13000rpm (such as 4000rpm), then again with 100~1500 mesh (such as 300
Mesh) filter cloth carry out negative pressure leaching.The suction filtration can add or be not added with filter aid.Such as plus filter aid, can be the activity of 0.1-10%
Charcoal, diatomite, perlite, cellulose etc..
In above-mentioned preparation method, in step (3), the activated carbon can be wood activated charcoal and/or active fruit shell carbon, institute
State active fruit shell carbon concretely cocoanut active charcoal；The addition volume of the wood activated charcoal and the active fruit shell carbon is institute
State the 0.1%~10% of clear liquid, concretely 0.75%~2.5%, 0.75%~2%, 1%~2.5%, 0.75%, 1%,
2% or 2.5%；The granularity of the activated carbon can be 8~400 mesh, and specifically, the granularity of the wood activated charcoal can be 8~300
Mesh, 8~30 mesh, 20~60 mesh, 200 mesh or 300 mesh；The granularity of the active fruit shell carbon can be 8~200 mesh, concretely 8~
16 mesh, 40~60 mesh.
The temperature of the adsorption treatment can be 4~99 DEG C, concretely 4 DEG C~80 DEG C, 4 DEG C, 40 DEG C, 50 DEG C or 80 DEG C,
Processing time can be 0.1~for 24 hours, concretely 4~16h, 4h or 16h.The adsorption treatment can be accompanied by stirring, including three kinds
Suction type, i.e. 1) standing adsorption after only stirring and evenly mixing, continue standing adsorption；2) continue stirring and adsorbing；3) intermittent stirring is inhaled
Attached, after only stirring and evenly mixing, every 10~60min (such as 30min) intermittent stirring is primary.The rotating speed of the stirring can be 1~
1000rpm, specifically can 300~350rpm, 300rpm or 350rpm.
The method that the separation of solid and liquid can use can be at least one of centrifugation, positive press filtration and negative pressure filtration, have
Body can be to carry out negative pressure leaching with the filter device of 100~30000 mesh (such as 300 mesh, 5000 mesh), such as be filtered successively using 300 mesh
Cloth and 5000 mesh filter plates carry out negative pressure leaching.
Above-mentioned preparation method, in step (4), the volume ratio of the clear liquid and ethyl alcohol can be 1：(0.1~100), specifically
Can be 1：(1~10), 1：3、1：1、1：5 or 1：10.The ethyl alcohol is using volumetric concentration as the aqueous solution of 70%~100% ethyl alcohol
The form of (edible ethanol that such as volumetric concentration is 95%) is added.The alcohol analysis can be carried out more than primary, and such as the alcohol is analysed
It may also include and the solid is redissolved in water, obtain beta glucan solution, the beta glucan solution is mixed with ethyl alcohol
The step of carrying out alcohol analysis again, collecting alcohol analysis solid again.In the analysis of alcohol again, the volume ratio of beta glucan solution and ethyl alcohol
Can be 1：(0.1~100), concretely 1：2、1：1；The ethyl alcohol is using volumetric concentration as the water-soluble of 70%~100% ethyl alcohol
The form of liquid (edible ethanol that such as volumetric concentration is 90% or 95%) is added.
Above-mentioned preparation method, in step (5), the available method of drying is heated drying, is dried under reduced pressure, is spray-dried
And any one of low temperature freeze-drying, concretely it is dried under 30~130 DEG C (such as 40~60 DEG C, 40 DEG C, 50 DEG C or 60 DEG C)
Constant weight.
The beta glucan being prepared by above-mentioned preparation method, also within the scope of the present invention.The beta glucan
Manufactured aqueous solution has high viscosity, high transparency.
The present invention also provides the preparation methods of beta glucan solution, include the following steps：The beta glucan is crushed
After be dissolved in the water, be filtered after stirring, collect clear liquid, you can obtain the beta glucan solution.
In above-mentioned preparation method, the dissolving can carry out under room temperature (15~30 DEG C) or heated condition, heating temperature
Degree can be 30~121 DEG C, concretely 40~80 DEG C, 40 DEG C, 70 DEG C or 80 DEG C.The stirring can be 1) not stir persistently；
2) lasting stirring, rotating speed can be 1~1000rpm (such as 600~800rpm, 600rpm or 800rpm), and the time can be 0.2~8h
(such as 2~6h, 2h, 4h or 6h).
The method that the filtering can use is at least one of positive press filtration, negative pressure filtration and membrane filtration, such as uses 1
The filter membrane of~150 μm (such as 5~10 μm, 5 μm or 10 μm) carries out negative pressure filtration or using 100~30000 mesh (such as 300 mesh)
Filter cloth or filter plate carry out negative pressure filtration.
The concentration of the beta glucan solution can be 0.01~10.0%, concretely 0.5~5.0%, 0.5%,
0.8%th, 1.0%, 2.0% or 5.0%.
The beta glucan solution being prepared by above-mentioned preparation method, also within the scope of the present invention.Wherein,
The beta glucan solution viscosity of 0.5% concentration can reach 600mPas, and light transmittance can reach more than 90%；0.8% concentration
Beta glucan solution viscosity can reach 1600mPas, light transmittance can reach more than 85%.
The present invention has the advantages that：
(1) enzymolysis process is not used, cost is relatively low.
(2) acidolysis or alkaline hydrolysis technique is not used, the functional structure of beta glucan will not be damaged.
(3) equipment and material are simply common, and simple process is easy, suitable for industrialization.
(4) beta glucan solution viscosity is big made from this law, and light transmittance is high, suitable for various applications.
(5) beta glucan dry product made from this law is water-soluble strong, and the beta glucan solution viscosity obtained after dissolving is big, light transmission
Rate is high.
Specific embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Zymotic fluid in following embodiments is made by the steps to obtain：
(1) schizophyllum commune actication of culture：By potato 200g/L, glucose 30g/L, sodium chloride 10g/L, agar 20g/L systems
Into plating medium, schizophyllum commune strain is inoculated on the plating medium, is put down in being cultivated 7 days in 25 DEG C of constant incubators
Plate mycelium；
(2) seed activation：By potato starch 100g/L, glucose 40g/L, yeast extract 2g/L, yeast extract 2g/L,
Fluid nutrient medium made of water is packed into shaking flask, and liquid amount 1/3 takes the tablet mycelium obtained by step (1) to be inoculated with the shaking flask, in
160rpm shake cultures culture 7 days in 25 DEG C of constant-temperature tables, as seed liquor；
(3) fermented and cultured：By glucose 50g/L, sucrose 50g/L, analysis for soybean powder 5g/L, yeast extract 2g/L, biphosphate
Potassium 0.5g/L, bitter salt 0.5g/L, ammonium sulfate 0.5g/L, potassium nitrate 6g/L, fermentation medium made of water add in hair
It sterilizes 15 minutes for 121 DEG C, then the seed liquor obtained by step (2) is inoculated in above-mentioned fermentation tank, 25 DEG C of constant temperature 300rpm in fermentation tank
Stir 4Lpm aerobic fementations culture 8 days.
Negative pressure filtration machine vacuum pump in following embodiments is purchased from Auto Science, model AP-9925.SCP-321#
Filter plate is purchased from Shenyang Great Wall filter board Co., Ltd.
In following embodiments, the measure of the concentration of beta glucan solution uses conventional sulfuric acid phynol method and Megazyme companies
Beta glucan dedicated test kit (β-Glucan Assay Kit (Yeast＆Mushroom)).
The assay method of the light transmittance of beta glucan solution is as follows in following embodiments：
(1) beta glucan solution example is taken to be placed in glass cuvette；
(2) using spectrophotometer, Detection wavelength is adjusted to 600nm；
(3) light transmittance of detection beta glucan solution example at that wavelength；
The assay method of dynamic viscosity is as follows：
(1) 200ml beta glucan solution examples is taken to be placed in 250ml beakers；
(2) beaker for filling beta glucan solution example is placed in water-bath, 25 DEG C of heat preservations；
(3) using rotational viscometer, respective rotor is selected according to beta glucan solution example concentration, it is dynamic at 25 DEG C of detection
Power viscosity.
Embodiment 1, purifying prepare high viscosity, high transparency beta glucan solution
In accordance with the following steps purifying prepare high viscosity, high transparency beta glucan solution：
(1) schizophyllary fermentating liquid is mixed with the distilled water of 4 times of volumes, digestion 8h at 60 DEG C.
(2) digestion liquid is centrifuged into 5min in 4000rpm, takes supernatant；By the filter cloth negative pressure filtration of 300 mesh of supernatant,
Take that filter out clear liquid for use.
(3) the digestion liquid after step (2) is filtered is heated to 50 DEG C, adds in the wood activated charcoal of 200 mesh simultaneously thereto
And 8~16 mesh cocoanut active charcoal, the addition volume of each activated carbon is the 1% of digesting liquor product.In 50 DEG C, 350rpm conditions
4h is persistently stirred down, it is for use after cooling；By the digestion clear liquid for being mixed with activated carbon that above-mentioned cooling is for use, successively using 300 mesh
Filter cloth and SCP-321# filter plates (about 1.5 μm of pore size) carry out negative pressure leaching, take the clear liquid after filtering for use.
(4) clear liquid after step (3) is filtered mixes (volume ratio 1 with the edible ethanol of 95% concentration：3) it, stirs, directly
It is precipitated to beta glucan.
(5) precipitate that step (4) obtains is placed in pallet with holes, is dried for 40 DEG C using Electric heat oven, until constant weight,
Obtain dry beta glucan.
(6) the beta glucan dried object for obtaining step (5) crushes, and weighs crushed material 5g, is dissolved in 1000ml ultra-pure waters
In, 600rpm stirring 2h are maintained, until beta glucan fully dissolves, obtain beta glucan solution；Above-mentioned beta glucan solution is made
Negative pressure leaching is carried out with 5 μm of filter membranes, you can obtains a concentration of 0.5% high viscosity, the beta glucan solution of high transparency.
Light transmittance of the beta glucan solution that the mass concentration is 0.5% under 600nm wavelength can reach 92.2%, move
Power viscosity can reach 710mPas (2# rotor 30rpm).
Embodiment 2, purifying prepare high viscosity, high transparency beta glucan solution
In accordance with the following steps purifying prepare high viscosity, high transparency beta glucan solution：
(1) schizophyllary fermentating liquid is mixed with the distilled water of 1 times of volume, high temperature digestion 0.5h at 90 DEG C.
(2) the schizophyllum commune fermentation digestion liquid 3000rpm centrifugation 60min obtained step (1), take supernatant；In supernatant
After adding in 2% diatomite, with the filter cloth negative pressure filtration of 300 mesh, take that filter out clear liquid for use.
(3) in the digestion liquid after step (2) filtering, 2% 200 mesh wood activated charcoals of digesting liquor product are added in,
5min is stirred at room temperature in 300rpm, until after stirring evenly, 16h is stood in 4 DEG C；By the above-mentioned mixed liquor containing activated carbon, make successively
Negative pressure leaching is carried out with 300 mesh filter clothes and SCP-321# (about 1.5 μm of pore size) filter plate, takes the clear liquid after filtering for use.
(4) clear liquid after step (3) is filtered mixes (volume ratio 1 with the edible ethanol of 95% concentration：1) it, and stirs,
Until beta glucan is precipitated.Appropriate pure water is added in into above-mentioned precipitate and is supplemented to the body that total volume reaches clear liquid before alcohol analysis
Product is stirred continuously until that alcohol analysis object fully dissolves.By the dissolved beta glucan solution edible ethanol with 95% concentration again
Mix (volume ratio 1：2) it, stirs, until beta glucan is precipitated.Beta glucan precipitate is transferred to 95% new concentration
In edible ethanol, soaked overnight obtains the beta glucan precipitate after ethyl alcohol washing.
(5) precipitate that step (4) obtains is placed in pallet, is placed in 40 DEG C of dryings in Electric heat oven, until constant weight, obtains
Beta glucan dried object.
(6) the beta glucan dried object for obtaining step (5) crushes, and weighs crushed material 8g, is dissolved in 1000ml preheatings
Into 40 DEG C of ultra-pure waters, 600rpm stirring 4h are maintained, until beta glucan fully dissolves, obtain beta glucan solution；Use 10 μ
M filter membranes carry out negative pressure leaching, you can obtain a concentration of 0.8% high viscosity, the beta glucan solution of high transparency.
Light transmittance of the beta glucan solution that the mass concentration is 0.8% under 600nm wavelength can reach 90.0%, move
Power viscosity can reach 2480mPas (4# rotor 30rpm).
Embodiment 3, purifying prepare high viscosity, high transparency beta glucan solution
In accordance with the following steps purifying prepare high viscosity, high transparency beta glucan solution：
(1) schizophyllary fermentating liquid is mixed with the tap water of 5 times of volumes, high temperature digestion 4h at 70 DEG C.
(2) the schizophyllum commune fermentation digestion liquid obtained step (1), carries out negative pressure filtrations using 8 layers of gauze, takes and filter out clearly
Liquid.
(3) wood activated charcoal and 8~16 purposes that 200 mesh are added in the digestion liquid after step (2) filtering are coconut activated
Charcoal, the addition volume of each activated carbon are the 0.75% of digesting liquor product.It is equal to activated carbon distribution that 5min is stirred at room temperature in 300rpm
After even, mixed liquor is heated to 50 DEG C, and stand 4h in 50 DEG C of heat preservations；It is filtered successively using 300 mesh filter clothes and SCP-321# later
Plate (about 1.5 μm of pore size) carries out negative pressure leaching, takes the clear liquid after filtering for use.
(4) clear liquid after step (3) is filtered mixes (volume ratio 1 with the edible ethanol of 95% concentration：5) it, stirs, directly
It is precipitated, precipitate is picked up, for use to beta glucan.
(5) precipitate that step (4) obtains is placed in pallet with holes, is dried for 50 DEG C using Electric heat oven, until constant weight,
Obtain dry beta glucan.
(6) the beta glucan dried object for obtaining step (5) crushes, and weighs 10g, is dissolved in 1000mL ultra-pure waters, is made
Beta glucan solution then in 121 DEG C of high pressure steam sterilization 30min, stands cooling, for use；β-Portugal after above-mentioned sterilizing is gathered
Sugar juice water proof is heated to 80 DEG C, 600rpm stirring 6h, until beta glucan fully dissolves；300 mesh filter clothes are reused to be born
Pressure filters, you can obtains a concentration of 1.0% high viscosity, the beta glucan solution of high transparency.
Light transmittance of the beta glucan solution that the mass concentration is 1.0% under 600nm wavelength can reach 81.1%, move
Power viscosity can reach 3100mPas (4# rotor 30rpm).
Embodiment 4, purifying prepare high viscosity, high transparency beta glucan solution
In accordance with the following steps purifying prepare high viscosity, high transparency beta glucan solution：
(1) schizophyllary fermentating liquid is mixed with the tap water of 10 times of volumes, high temperature digestion 2h at 50 DEG C.
(2) the schizophyllary fermentating liquid digestion liquid for obtaining step (1), 5000rpm centrifugation 10min, takes supernatant for use.
(3) activated carbon of 300 mesh is added in the digestion liquid after step (2) centrifugation, addition volume is digesting liquor
Long-pending 2.5%.300rpm after 5min is stirred at room temperature, is heated to 80 DEG C, and stand 4h in 80 DEG C of heat preservations, during which the interval per 30min
Stirring is primary；0.5% diatomite into the above-mentioned digestion clear liquid for being mixed with activated carbon, will be added in, 300 mesh are used successively after mixing
Filter cloth and SCP-321# filter plates (about 1.5 μm of pore size) carry out negative pressure leaching, take the clear liquid after filtering for use.
(4) clear liquid after step (3) is filtered mixes (volume ratio 1 with the edible ethanol of 95% concentration：10) it, stirs,
Until beta glucan is precipitated.Above-mentioned precipitate is dissolved in in the pure water of precipitate same volume, being stirred continuously until at 80 DEG C
Alcohol analysis object fully dissolves.Edible ethanol of the dissolved beta glucan solution again with 95% concentration is mixed into (volume ratio 1：
1) it, stirs, until beta glucan is precipitated.
(5) precipitate that step (4) obtains is placed in pallet with holes, using 60 DEG C of dryings of Electric heat oven, until constant weight,
Obtain dry beta glucan.
(6) the beta glucan dried object for obtaining step (5) crushes, and weighs crushed material 5g and 8g, is dissolved in 1000mL respectively
In ultra-pure water, then in 115 DEG C of high pressure steam sterilization 20min；Beta glucan solution water proof after above-mentioned sterilizing is heated to 70
DEG C, 800rpm stirring 4h are maintained, until beta glucan fully dissolves；It reuses 10 μm of filter membranes to be filtered by vacuum, you can respectively
Obtain a concentration of 0.5% and 0.8% high viscosity, the beta glucan solution of high transparency.
Light transmittance of the beta glucan solution that the mass concentration is 0.5% under 600nm wavelength can reach 93.1%, move
Power viscosity can reach 740mPas (2# rotor 30rpm)；The beta glucan solution that mass concentration is 0.8% is in 600nm wavelength
Under light transmittance can reach 87.5%, dynamic viscosity can reach 2150mPas (2# rotor 30rpm).
Claims (10)
1. a kind of preparation method of beta glucan, includes the following steps：
(1) schizophyllary fermentating liquid with water is mixed and carries out digestion, obtain digestion liquid；
(2) separation of solid and liquid is carried out to step (1) the digestion liquid, takes clear liquid；
(3) activated carbon is added in the clear liquid obtained in step (2), adsorption treatment is carried out, obtains containing carbon mixture；It is mixed to described containing charcoal
It closes object and carries out separation of solid and liquid, take clear liquid；
(4) clear liquid for obtaining step (3) is mixed with ethyl alcohol carries out alcohol analysis, collects the solid of precipitation；
(5) solid described in step (4) is dried, you can obtain the beta glucan.
2. preparation method according to claim 1, it is characterised in that：In step (1), the schizophyllary fermentating liquid and water
Volume ratio is 1：(0.1~100)；The temperature of the digestion is 30~121 DEG C, and the time is 0.2~120h.
3. preparation method according to claim 1 or 2, it is characterised in that：In step (2), the separation of solid and liquid for centrifugation,
At least one of positive press filtration and negative pressure filtration.
4. according to the preparation method described in claim 1-3, it is characterised in that：In step (3), the activated carbon is wooden activity
Charcoal and active fruit shell carbon；The addition volume of the wood activated charcoal and the active fruit shell carbon be the clear liquid 0.1%~
10%；The granularity of the activated carbon is 8~400 mesh；And/or
The temperature of the adsorption treatment is 4~99 DEG C, the time for 0.1~for 24 hours；And/or
The separation of solid and liquid is at least one of centrifugation, positive press filtration and negative pressure filtration.
5. according to the preparation method described in any one of claim 1-4, it is characterised in that：In step (4), the clear liquid and second
The volume ratio of alcohol is 1：(0.1~100)；The ethyl alcohol is in the form of the aqueous solution for the ethyl alcohol that volumetric concentration is 75%~100%
It is added；The alcohol analysis is carried out more than primary.
6. preparation method according to any one of claims 1-5, it is characterised in that：In step (5), the drying is heat
Dry, be dried under reduced pressure, being spray-dried and low temperature freeze-drying any one of.
7. the beta glucan that the preparation method described in any one of claim 1-6 is prepared.
8. a kind of preparation method of beta glucan solution, it includes the following steps：Water is dissolved in after the beta glucan is crushed
In, it is filtered after stirring, collects clear liquid, you can obtain the beta glucan solution.
9. preparation method according to claim 8, it is characterised in that：It is described to be filtered into centrifugation, positive press filtration and negative pressure mistake
At least one of filter.
10. the beta glucan solution that the preparation method described in claim 8 or 9 is prepared.
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