CN110073000A - 遗传擦除子 - Google Patents

遗传擦除子 Download PDF

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Publication number
CN110073000A
CN110073000A CN201780057742.XA CN201780057742A CN110073000A CN 110073000 A CN110073000 A CN 110073000A CN 201780057742 A CN201780057742 A CN 201780057742A CN 110073000 A CN110073000 A CN 110073000A
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cells
cell
genetic construct
engineered genetic
recombinase
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CN201780057742.XA
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Chinese (zh)
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T·K-T·卢
R·王
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Senti Biosciences Inc
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Senti Biosciences Inc
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  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CN201780057742.XA 2016-07-26 2017-07-26 遗传擦除子 Pending CN110073000A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662366755P 2016-07-26 2016-07-26
US62/366,755 2016-07-26
PCT/US2017/043931 WO2018022747A1 (en) 2016-07-26 2017-07-26 Genetic erasers

Publications (1)

Publication Number Publication Date
CN110073000A true CN110073000A (zh) 2019-07-30

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Application Number Title Priority Date Filing Date
CN201780057742.XA Pending CN110073000A (zh) 2016-07-26 2017-07-26 遗传擦除子
CN201780058989.3A Pending CN110088285A (zh) 2016-07-26 2017-07-26 时空调节子

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Application Number Title Priority Date Filing Date
CN201780058989.3A Pending CN110088285A (zh) 2016-07-26 2017-07-26 时空调节子

Country Status (8)

Country Link
US (2) US20190169634A1 (https=)
EP (2) EP3491138A1 (https=)
JP (2) JP2019521717A (https=)
KR (2) KR20190053179A (https=)
CN (2) CN110073000A (https=)
AU (2) AU2017302589A1 (https=)
CA (2) CA3031673A1 (https=)
WO (2) WO2018022747A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025228035A1 (zh) * 2024-04-30 2025-11-06 深圳市深研生物科技有限公司 利用膜蛋白进行细胞分选的方法

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11446398B2 (en) 2016-04-11 2022-09-20 Obsidian Therapeutics, Inc. Regulated biocircuit systems
EP3235908A1 (en) 2016-04-21 2017-10-25 Ecole Normale Superieure De Lyon Methods for selectively modulating the activity of distinct subtypes of cells
CA3062426A1 (en) 2017-05-08 2018-11-15 Flagship Pioneering Innovations V, Inc. Compositions for facilitating membrane fusion and uses thereof
SG11202011015QA (en) * 2018-05-15 2020-12-30 Flagship Pioneering Innovations V Inc Fusosome compositions and uses thereof
WO2019241315A1 (en) 2018-06-12 2019-12-19 Obsidian Therapeutics, Inc. Pde5 derived regulatory constructs and methods of use in immunotherapy
CN112955174A (zh) 2018-07-09 2021-06-11 旗舰先锋创新V股份有限公司 融合剂脂质体组合物和其用途
US20210386788A1 (en) 2018-10-24 2021-12-16 Obsidian Therapeutics, Inc. Er tunable protein regulation
CN113631718A (zh) 2018-11-14 2021-11-09 旗舰先锋创新V股份有限公司 用于特定隔室货物递送的组合物和方法
WO2021100034A1 (en) * 2019-11-19 2021-05-27 Protalix Ltd. Removal of constructs from transformed cells
GB202006462D0 (en) * 2020-05-04 2020-06-17 Mote Res Limited Modifying genomes with integrase
KR20240028975A (ko) 2021-04-08 2024-03-05 사나 바이오테크놀로지, 인크. Cd8-특이적 항체 구조체들 및 이의 조성물
CN121693564A (zh) 2023-06-26 2026-03-17 夏威夷大学 进化的整合酶和其用于基因组编辑的方法
CN118813656B (zh) * 2024-06-19 2025-06-03 山东大学 一种大片段dna级联组装的方法、系统及应用
GB2642222A (en) * 2024-06-26 2026-01-07 E V A Biosystems Ltd A gene cassette and uses therefor

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6537805B1 (en) * 1998-07-30 2003-03-25 Harald Von Melchner Self-deleting vectors
WO2008019052A2 (en) * 2006-08-03 2008-02-14 Numira Biosciences, Inc. Methods and compositions for identifying biomarkers
US20100229254A1 (en) * 2009-03-04 2010-09-09 Kmita Marie Method for targeted cell ablation
WO2014093852A1 (en) * 2012-12-13 2014-06-19 Massachusetts Institute Of Technology Recombinase-based logic and memory systems

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7189506B1 (en) * 1999-03-03 2007-03-13 Genelabs Technologies, Inc. DNA binding compound-mediated molecular switch system
US20060265771A1 (en) * 2005-05-17 2006-11-23 Lewis David L Monitoring microrna expression and function
CA2609142C (en) * 2005-05-27 2016-02-09 Fondazione Centro San Raffaele Del Monte Tabor Therapeutic gene vectors comprising mirna target sequences
WO2006128245A1 (en) * 2005-06-03 2006-12-07 Southern Adelaide Health Service-Flinders Medical Centre Targeting cells with altered microrna expression
US20070054872A1 (en) * 2005-08-24 2007-03-08 Mirus Bio Corporation Regulatable or conditional expression systems
US20090286242A1 (en) * 2007-12-10 2009-11-19 Cold Spring Harbor Laboratory MicroRNA Expression Profiling and Uses Thereof
CN102099472A (zh) * 2008-05-19 2011-06-15 新加坡科技研究局 使基因表达靶向于神经胶质瘤的核酸分子和方法
EP3444346B1 (en) * 2010-04-23 2022-07-27 University of Massachusetts Aav-based treatment of cholesterol-related disorders
WO2012056440A1 (en) * 2010-10-28 2012-05-03 Nanodoc Ltd. COMPOSITIONS AND METHODS FOR ACTIVATING EXPRESSION BY A SPECIFIC ENDOGENOUS miRNA
CN102719556A (zh) * 2011-03-29 2012-10-10 北京五加和分子医学研究所有限公司 基于AAV载体的高通量miRNA活性检测方法及其应用
WO2012131048A1 (en) * 2011-03-31 2012-10-04 Royal College Of Surgeons In Ireland Treatment and prognosis of solid tumour cancers
CN102358901A (zh) * 2011-11-11 2012-02-22 天津托普泰克生物科技有限公司 Has-miR-520e的抗肿瘤用途
EP2684962A1 (en) * 2012-07-10 2014-01-15 Commissariat A L'energie Atomique Et Aux Energies Alternatives Vector for the selective silencing of a gene in astrocytes
EP2948550A1 (en) * 2013-01-28 2015-12-02 Council of Scientific & Industrial Research METHOD FOR INHIBITING TUMOR GROWTH THROUGH RNA-INTERFERENCE USING LIPOSOMALLY ASSOCIATED CDC20 siRNA
FR3004463A1 (fr) * 2013-04-11 2014-10-17 Genethon Systeme d'expression pour une therapie genique selective
CN107073051B (zh) * 2014-10-21 2021-08-24 马萨诸塞大学 重组aav变体及其用途
JP2016088884A (ja) * 2014-11-04 2016-05-23 国立大学法人岐阜大学 腫瘍治療用組成物
CN108350445A (zh) 2015-06-19 2018-07-31 麻省理工学院 肿瘤免疫治疗
WO2017096363A1 (en) * 2015-12-04 2017-06-08 Board Of Regents, The University Of Texas System Reporter system for detecting and targeting activated cells

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6537805B1 (en) * 1998-07-30 2003-03-25 Harald Von Melchner Self-deleting vectors
WO2008019052A2 (en) * 2006-08-03 2008-02-14 Numira Biosciences, Inc. Methods and compositions for identifying biomarkers
US20100229254A1 (en) * 2009-03-04 2010-09-09 Kmita Marie Method for targeted cell ablation
WO2014093852A1 (en) * 2012-12-13 2014-06-19 Massachusetts Institute Of Technology Recombinase-based logic and memory systems

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SHIGEMI HAYASHI等: "Efficient Recombination in Diverse Tissues by a Tamoxifen-Inducible Form of Cre: A Tool for Temporally Regulated Gene Activation/Inactivation in the Mouse", 《DEV BIOL》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025228035A1 (zh) * 2024-04-30 2025-11-06 深圳市深研生物科技有限公司 利用膜蛋白进行细胞分选的方法

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