CN110073000A - 遗传擦除子 - Google Patents
遗传擦除子 Download PDFInfo
- Publication number
- CN110073000A CN110073000A CN201780057742.XA CN201780057742A CN110073000A CN 110073000 A CN110073000 A CN 110073000A CN 201780057742 A CN201780057742 A CN 201780057742A CN 110073000 A CN110073000 A CN 110073000A
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- Prior art keywords
- cells
- cell
- genetic construct
- engineered genetic
- recombinase
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C12N15/09—Recombinant DNA-technology
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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- C12N5/06—Animal cells or tissues; Human cells or tissues
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- C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
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- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C12N2310/00—Structure or type of the nucleic acid
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
- C12N2310/141—MicroRNAs, miRNAs
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
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- C12N2310/3513—Protein; Peptide
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- C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Hematology (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662366755P | 2016-07-26 | 2016-07-26 | |
| US62/366,755 | 2016-07-26 | ||
| PCT/US2017/043931 WO2018022747A1 (en) | 2016-07-26 | 2017-07-26 | Genetic erasers |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110073000A true CN110073000A (zh) | 2019-07-30 |
Family
ID=59523304
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201780057742.XA Pending CN110073000A (zh) | 2016-07-26 | 2017-07-26 | 遗传擦除子 |
| CN201780058989.3A Pending CN110088285A (zh) | 2016-07-26 | 2017-07-26 | 时空调节子 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201780058989.3A Pending CN110088285A (zh) | 2016-07-26 | 2017-07-26 | 时空调节子 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20190169634A1 (https=) |
| EP (2) | EP3491138A1 (https=) |
| JP (2) | JP2019521717A (https=) |
| KR (2) | KR20190053179A (https=) |
| CN (2) | CN110073000A (https=) |
| AU (2) | AU2017302589A1 (https=) |
| CA (2) | CA3031673A1 (https=) |
| WO (2) | WO2018022747A1 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025228035A1 (zh) * | 2024-04-30 | 2025-11-06 | 深圳市深研生物科技有限公司 | 利用膜蛋白进行细胞分选的方法 |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11446398B2 (en) | 2016-04-11 | 2022-09-20 | Obsidian Therapeutics, Inc. | Regulated biocircuit systems |
| EP3235908A1 (en) | 2016-04-21 | 2017-10-25 | Ecole Normale Superieure De Lyon | Methods for selectively modulating the activity of distinct subtypes of cells |
| CA3062426A1 (en) | 2017-05-08 | 2018-11-15 | Flagship Pioneering Innovations V, Inc. | Compositions for facilitating membrane fusion and uses thereof |
| SG11202011015QA (en) * | 2018-05-15 | 2020-12-30 | Flagship Pioneering Innovations V Inc | Fusosome compositions and uses thereof |
| WO2019241315A1 (en) | 2018-06-12 | 2019-12-19 | Obsidian Therapeutics, Inc. | Pde5 derived regulatory constructs and methods of use in immunotherapy |
| CN112955174A (zh) | 2018-07-09 | 2021-06-11 | 旗舰先锋创新V股份有限公司 | 融合剂脂质体组合物和其用途 |
| US20210386788A1 (en) | 2018-10-24 | 2021-12-16 | Obsidian Therapeutics, Inc. | Er tunable protein regulation |
| CN113631718A (zh) | 2018-11-14 | 2021-11-09 | 旗舰先锋创新V股份有限公司 | 用于特定隔室货物递送的组合物和方法 |
| WO2021100034A1 (en) * | 2019-11-19 | 2021-05-27 | Protalix Ltd. | Removal of constructs from transformed cells |
| GB202006462D0 (en) * | 2020-05-04 | 2020-06-17 | Mote Res Limited | Modifying genomes with integrase |
| KR20240028975A (ko) | 2021-04-08 | 2024-03-05 | 사나 바이오테크놀로지, 인크. | Cd8-특이적 항체 구조체들 및 이의 조성물 |
| CN121693564A (zh) | 2023-06-26 | 2026-03-17 | 夏威夷大学 | 进化的整合酶和其用于基因组编辑的方法 |
| CN118813656B (zh) * | 2024-06-19 | 2025-06-03 | 山东大学 | 一种大片段dna级联组装的方法、系统及应用 |
| GB2642222A (en) * | 2024-06-26 | 2026-01-07 | E V A Biosystems Ltd | A gene cassette and uses therefor |
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| US6537805B1 (en) * | 1998-07-30 | 2003-03-25 | Harald Von Melchner | Self-deleting vectors |
| WO2008019052A2 (en) * | 2006-08-03 | 2008-02-14 | Numira Biosciences, Inc. | Methods and compositions for identifying biomarkers |
| US20100229254A1 (en) * | 2009-03-04 | 2010-09-09 | Kmita Marie | Method for targeted cell ablation |
| WO2014093852A1 (en) * | 2012-12-13 | 2014-06-19 | Massachusetts Institute Of Technology | Recombinase-based logic and memory systems |
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| US7189506B1 (en) * | 1999-03-03 | 2007-03-13 | Genelabs Technologies, Inc. | DNA binding compound-mediated molecular switch system |
| US20060265771A1 (en) * | 2005-05-17 | 2006-11-23 | Lewis David L | Monitoring microrna expression and function |
| CA2609142C (en) * | 2005-05-27 | 2016-02-09 | Fondazione Centro San Raffaele Del Monte Tabor | Therapeutic gene vectors comprising mirna target sequences |
| WO2006128245A1 (en) * | 2005-06-03 | 2006-12-07 | Southern Adelaide Health Service-Flinders Medical Centre | Targeting cells with altered microrna expression |
| US20070054872A1 (en) * | 2005-08-24 | 2007-03-08 | Mirus Bio Corporation | Regulatable or conditional expression systems |
| US20090286242A1 (en) * | 2007-12-10 | 2009-11-19 | Cold Spring Harbor Laboratory | MicroRNA Expression Profiling and Uses Thereof |
| CN102099472A (zh) * | 2008-05-19 | 2011-06-15 | 新加坡科技研究局 | 使基因表达靶向于神经胶质瘤的核酸分子和方法 |
| EP3444346B1 (en) * | 2010-04-23 | 2022-07-27 | University of Massachusetts | Aav-based treatment of cholesterol-related disorders |
| WO2012056440A1 (en) * | 2010-10-28 | 2012-05-03 | Nanodoc Ltd. | COMPOSITIONS AND METHODS FOR ACTIVATING EXPRESSION BY A SPECIFIC ENDOGENOUS miRNA |
| CN102719556A (zh) * | 2011-03-29 | 2012-10-10 | 北京五加和分子医学研究所有限公司 | 基于AAV载体的高通量miRNA活性检测方法及其应用 |
| WO2012131048A1 (en) * | 2011-03-31 | 2012-10-04 | Royal College Of Surgeons In Ireland | Treatment and prognosis of solid tumour cancers |
| CN102358901A (zh) * | 2011-11-11 | 2012-02-22 | 天津托普泰克生物科技有限公司 | Has-miR-520e的抗肿瘤用途 |
| EP2684962A1 (en) * | 2012-07-10 | 2014-01-15 | Commissariat A L'energie Atomique Et Aux Energies Alternatives | Vector for the selective silencing of a gene in astrocytes |
| EP2948550A1 (en) * | 2013-01-28 | 2015-12-02 | Council of Scientific & Industrial Research | METHOD FOR INHIBITING TUMOR GROWTH THROUGH RNA-INTERFERENCE USING LIPOSOMALLY ASSOCIATED CDC20 siRNA |
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| WO2017096363A1 (en) * | 2015-12-04 | 2017-06-08 | Board Of Regents, The University Of Texas System | Reporter system for detecting and targeting activated cells |
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- 2017-07-26 EP EP17754865.8A patent/EP3491138A1/en not_active Withdrawn
- 2017-07-26 JP JP2019526195A patent/JP2019521717A/ja active Pending
- 2017-07-26 US US16/319,786 patent/US20190169634A1/en not_active Abandoned
- 2017-07-26 CN CN201780057742.XA patent/CN110073000A/zh active Pending
- 2017-07-26 KR KR1020197005575A patent/KR20190053179A/ko not_active Withdrawn
- 2017-07-26 AU AU2017302589A patent/AU2017302589A1/en not_active Abandoned
- 2017-07-26 WO PCT/US2017/043938 patent/WO2018022749A1/en not_active Ceased
- 2017-07-26 KR KR1020197005579A patent/KR20190053180A/ko not_active Ceased
- 2017-07-26 CA CA3031673A patent/CA3031673A1/en not_active Abandoned
- 2017-07-26 CA CA3031670A patent/CA3031670A1/en active Pending
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- 2017-07-26 CN CN201780058989.3A patent/CN110088285A/zh active Pending
- 2017-07-26 US US16/319,791 patent/US20190233844A1/en not_active Abandoned
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| WO2025228035A1 (zh) * | 2024-04-30 | 2025-11-06 | 深圳市深研生物科技有限公司 | 利用膜蛋白进行细胞分选的方法 |
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| KR20190053180A (ko) | 2019-05-17 |
| AU2017302587A1 (en) | 2019-02-07 |
| US20190233844A1 (en) | 2019-08-01 |
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| JP2019521717A (ja) | 2019-08-08 |
| KR20190053179A (ko) | 2019-05-17 |
| CA3031670A1 (en) | 2018-02-01 |
| US20190169634A1 (en) | 2019-06-06 |
| EP3491138A1 (en) | 2019-06-05 |
| AU2017302589A1 (en) | 2019-02-28 |
| WO2018022747A1 (en) | 2018-02-01 |
| CN110088285A (zh) | 2019-08-02 |
| WO2018022749A1 (en) | 2018-02-01 |
| EP3491137A1 (en) | 2019-06-05 |
| CA3031673A1 (en) | 2018-02-01 |
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