CN110066730A - 切向流动灌注系统 - Google Patents
切向流动灌注系统 Download PDFInfo
- Publication number
- CN110066730A CN110066730A CN201811359986.6A CN201811359986A CN110066730A CN 110066730 A CN110066730 A CN 110066730A CN 201811359986 A CN201811359986 A CN 201811359986A CN 110066730 A CN110066730 A CN 110066730A
- Authority
- CN
- China
- Prior art keywords
- bioreactor
- inlet
- outlet
- retentate
- reciprocating pump
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000012465 retentate Substances 0.000 claims abstract description 71
- 239000012466 permeate Substances 0.000 claims abstract description 31
- 230000010412 perfusion Effects 0.000 claims abstract description 27
- 230000001954 sterilising effect Effects 0.000 claims abstract description 14
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 14
- 239000012530 fluid Substances 0.000 claims description 45
- 238000000034 method Methods 0.000 claims description 29
- 239000012528 membrane Substances 0.000 claims description 21
- 230000003204 osmotic effect Effects 0.000 claims description 20
- 239000012510 hollow fiber Substances 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 5
- 239000006143 cell culture medium Substances 0.000 claims description 4
- 238000001471 micro-filtration Methods 0.000 claims description 4
- 239000013076 target substance Substances 0.000 claims description 4
- 238000000108 ultra-filtration Methods 0.000 claims description 4
- 238000013019 agitation Methods 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 2
- 239000012531 culture fluid Substances 0.000 description 8
- 239000007788 liquid Substances 0.000 description 8
- 239000000835 fiber Substances 0.000 description 6
- 238000001914 filtration Methods 0.000 description 5
- 238000004113 cell culture Methods 0.000 description 4
- 230000001276 controlling effect Effects 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 238000009295 crossflow filtration Methods 0.000 description 3
- 230000002706 hydrostatic effect Effects 0.000 description 3
- 230000033001 locomotion Effects 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- 238000011001 backwashing Methods 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 229940072221 immunoglobulins Drugs 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 229920002492 poly(sulfone) Polymers 0.000 description 2
- -1 polypropylene Polymers 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 239000004696 Poly ether ether ketone Substances 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 238000009455 aseptic packaging Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- JUPQTSLXMOCDHR-UHFFFAOYSA-N benzene-1,4-diol;bis(4-fluorophenyl)methanone Chemical compound OC1=CC=C(O)C=C1.C1=CC(F)=CC=C1C(=O)C1=CC=C(F)C=C1 JUPQTSLXMOCDHR-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 229920006351 engineering plastic Polymers 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229920001038 ethylene copolymer Polymers 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000013206 minimal dilution Methods 0.000 description 1
- 238000010943 off-gassing Methods 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- 229920002530 polyetherether ketone Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000004945 silicone rubber Substances 0.000 description 1
- 238000004513 sizing Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000013077 target material Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/02—Percolation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/18—Apparatus therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/22—Controlling or regulating
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
- B01D65/02—Membrane cleaning or sterilisation ; Membrane regeneration
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/12—Pulsatile flow
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/16—Hollow fibers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B23/00—Pumping installations or systems
- F04B23/02—Pumping installations or systems having reservoirs
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B43/00—Machines, pumps, or pumping installations having flexible working members
- F04B43/0009—Special features
- F04B43/0081—Special features systems, control, safety measures
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B43/00—Machines, pumps, or pumping installations having flexible working members
- F04B43/02—Machines, pumps, or pumping installations having flexible working members having plate-like flexible members, e.g. diaphragms
- F04B43/06—Pumps having fluid drive
- F04B43/073—Pumps having fluid drive the actuating fluid being controlled by at least one valve
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B53/00—Component parts, details or accessories not provided for in, or of interest apart from, groups F04B1/00 - F04B23/00 or F04B39/00 - F04B47/00
- F04B53/20—Filtering
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2313/00—Details relating to membrane modules or apparatus
- B01D2313/24—Specific pressurizing or depressurizing means
- B01D2313/243—Pumps
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2313/00—Details relating to membrane modules or apparatus
- B01D2313/44—Cartridge types
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2321/00—Details relating to membrane cleaning, regeneration, sterilization or to the prevention of fouling
- B01D2321/04—Backflushing
Landscapes
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Water Supply & Treatment (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mechanical Engineering (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE1251091-3 | 2012-09-27 | ||
| SE1251091 | 2012-09-27 | ||
| CN201380050422.3A CN104640973A (zh) | 2012-09-27 | 2013-09-25 | 切向流动灌注系统 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201380050422.3A Division CN104640973A (zh) | 2012-09-27 | 2013-09-25 | 切向流动灌注系统 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110066730A true CN110066730A (zh) | 2019-07-30 |
Family
ID=50388729
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811359986.6A Pending CN110066730A (zh) | 2012-09-27 | 2013-09-25 | 切向流动灌注系统 |
| CN201380050422.3A Pending CN104640973A (zh) | 2012-09-27 | 2013-09-25 | 切向流动灌注系统 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201380050422.3A Pending CN104640973A (zh) | 2012-09-27 | 2013-09-25 | 切向流动灌注系统 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US9663753B2 (https=) |
| EP (1) | EP2900804B1 (https=) |
| JP (1) | JP6321661B2 (https=) |
| CN (2) | CN110066730A (https=) |
| IN (1) | IN2015DN01067A (https=) |
| WO (1) | WO2014051503A1 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114616044A (zh) * | 2019-10-10 | 2022-06-10 | 伊康斯博尔公司 | 一种具有内置阀门的膜式气体分离器及其打开和关闭方法 |
Families Citing this family (53)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10711238B2 (en) | 2012-10-02 | 2020-07-14 | Repligen Corporation | Method for proliferation of cells within a bioreactor using a disposable pumphead and filter assembly |
| BR112016005545B1 (pt) * | 2013-09-16 | 2021-06-01 | Genzyme Corporation | Método de processamento de uma cultura de células de mamífero |
| JP6530171B2 (ja) * | 2014-09-09 | 2019-06-12 | 旭化成メディカル株式会社 | 培養産生物の回収方法 |
| EP3215261B1 (en) * | 2014-11-07 | 2021-12-15 | Genesis Technologies, LLC | Linear reciprocating actuator |
| KR20230156168A (ko) * | 2015-08-08 | 2023-11-13 | 스토베 게엠베하 | 생물학적 활동을 지원하는 일회용 생물 공정 시스템 |
| US11110398B2 (en) | 2015-08-20 | 2021-09-07 | Cytiva Sweden Ab | Method for enhancing filtration yields in tangential flow filtration system |
| GB201518767D0 (en) | 2015-10-22 | 2015-12-09 | Univ Newcastle | Cell culture |
| US11156219B2 (en) * | 2015-11-10 | 2021-10-26 | Repligen Corporation | Disposable alternating tangential flow filtration units |
| EP3386613B1 (en) | 2015-12-07 | 2025-05-21 | Cytiva Sweden AB | Unit for treatment of a bioprocess liquid with disposable flow path elements |
| ITUB20160272A1 (it) * | 2016-01-22 | 2017-07-22 | Univ Degli Studi Di Palermo | Bioreattore a perfusione autosufficiente monouso per crescite cellulari 3D |
| US12169034B2 (en) * | 2016-02-26 | 2024-12-17 | Cytiva Sweden Ab | Method for pressure control in crossflow filtration |
| CN105950468B (zh) * | 2016-05-23 | 2018-03-02 | 西安交通大学 | 一种用于灌流的生物反应器以及用于渗透的生物反应器 |
| US11473047B2 (en) | 2016-07-19 | 2022-10-18 | The Automation Partnership (Cambridge) Limited | Liquid filtration and pump system |
| CN109415669B (zh) * | 2016-07-19 | 2023-01-31 | 自动化合作关系（剑桥）有限公司 | 可逆液体过滤系统 |
| JP6890389B2 (ja) * | 2016-08-29 | 2021-06-18 | 株式会社日立製作所 | 送液装置、及びそれを用いた細胞培養装置 |
| DK179641B1 (en) * | 2017-02-10 | 2019-03-06 | Sani Membranes Aps | Vibrating filter plate assembly device |
| TWI675696B (zh) * | 2017-06-01 | 2019-11-01 | 美商Ｅｍｄ密理博公司 | 用於灌注應用之切向流過濾裝置 |
| US10774297B2 (en) * | 2017-08-03 | 2020-09-15 | Repligen Corporation | Method of actuation of an alternating tangential flow diaphragm pump |
| JP2020534829A (ja) * | 2017-09-27 | 2020-12-03 | ユニバーセルズ エス．エー．Ｕｎｉｖｅｒｃｅｌｌｓ Ｓ．Ａ． | ウイルスワクチンなどの生体分子の製造システムおよび製造方法 |
| EP3700505A4 (en) * | 2017-10-26 | 2021-01-06 | Repligen Corporation | MICRO-ALTERNATIVE TANGENTIAL FLOW INFUSION FILTER, PROCESSING CONTAINER, AND METHODS OF USE THEREOF |
| US10792594B2 (en) | 2017-12-28 | 2020-10-06 | Replegin Corporation | Dual pumping arrangement for a hollow fiber filter |
| WO2019139842A1 (en) * | 2018-01-12 | 2019-07-18 | Formulatrix, Inc. | Tangential flow filtration (tff) system and disposable tff unit that includes an integrated pump apparatus |
| US12077743B2 (en) | 2018-02-09 | 2024-09-03 | Global Life Sciences Solutions Usa Llc | Apparatus for fluid line management in a bioprocessing system |
| JP7520322B2 (ja) * | 2018-02-09 | 2024-07-23 | グローバル・ライフ・サイエンシズ・ソリューションズ・ユーエスエー・エルエルシー | 細胞療法のためのバイオプロセッシング方法 |
| US11932842B2 (en) | 2018-02-09 | 2024-03-19 | Global Life Sciences Solutions Usa Llc | Bioprocessing apparatus |
| US11920119B2 (en) | 2018-02-09 | 2024-03-05 | Global Life Sciences Solutions Usa Llc | Systems and methods for bioprocessing |
| US12252682B2 (en) | 2018-02-09 | 2025-03-18 | Global Life Sciences Solutions Usa Llc | System and method for fluid flow management in a bioprocessing system |
| US10889792B2 (en) | 2018-02-09 | 2021-01-12 | Global Life Sciences Solutions Usa Llc | Cell expansion vessel systems and methods |
| SG11202006223PA (en) * | 2018-02-27 | 2020-07-29 | Mitsubishi Electric Corp | Aeration amount control system and aeration amount control method |
| KR102930184B1 (ko) * | 2018-07-27 | 2026-02-23 | 유니버셀스 테크놀로지스 에스.에이. | 생체분자 생산 시스템 및 방법 |
| US12565635B2 (en) * | 2018-08-29 | 2026-03-03 | Cytiva Sweden Ab | Method of operation of a perfusion system |
| GB201816897D0 (en) * | 2018-10-17 | 2018-11-28 | Ge Healthcare Bio Sciences Corp | A Bioreactor System |
| CN109735448A (zh) * | 2019-02-02 | 2019-05-10 | 上海天引生物科技有限公司 | 一种细胞体外流体剪切力刺激培养装置及其方法 |
| WO2020163454A1 (en) * | 2019-02-08 | 2020-08-13 | Lonza Walkersville, Inc. | Cell concentration methods and devices for use in automated bioreactors |
| US12509654B2 (en) | 2019-05-02 | 2025-12-30 | Global Life Sciences Solutions Usa Llc | Bioprocessing system and consumable bag for a bioprocessing system |
| GB201908612D0 (en) | 2019-06-17 | 2019-07-31 | Ge Healthcare Bio Sciences Ab | A method for separating biomolecules |
| JP7340312B2 (ja) * | 2019-08-05 | 2023-09-07 | レプリゲン・コーポレーション | 交互接線流を用いた連続処理のための装置、システム、および方法 |
| EP4013845A1 (en) * | 2019-08-12 | 2022-06-22 | Cytiva Sweden AB | Systems and methods for perfusion control in bioreactors |
| EP3835402B1 (de) * | 2019-12-09 | 2025-10-15 | Sartorius Stedim Biotech GmbH | Modulares prozessiersystem |
| EP4087910A1 (en) * | 2020-01-07 | 2022-11-16 | Cytiva Sweden AB | Systems and method for controlling fluid flow in bioreactors |
| US20230120489A1 (en) * | 2020-03-06 | 2023-04-20 | Yukuan Shang | Perfusion module and perfusion culture system |
| CN111534411A (zh) * | 2020-04-03 | 2020-08-14 | 利穗科技(苏州)有限公司 | 一种交替切向流罐装培养装置及其控制方法 |
| CN112322496B (zh) * | 2021-01-05 | 2021-04-23 | 北京百普赛斯生物科技股份有限公司 | 一种新冠病毒s1蛋白的灌流生产系统及方法 |
| EP4291323A1 (en) * | 2021-02-11 | 2023-12-20 | Sani Membranes A/S | Filtration device |
| EP4313356A4 (en) | 2021-04-16 | 2025-02-19 | Repligen Corporation | Filtration system and method |
| JP7767029B2 (ja) * | 2021-05-28 | 2025-11-11 | ダイセン・メンブレン・システムズ株式会社 | 細胞外小胞の分離精製方法 |
| JP7716233B2 (ja) * | 2021-05-28 | 2025-07-31 | ダイセン・メンブレン・システムズ株式会社 | 微小有用物質の分離精製方法と分離精製装置 |
| CN113265330B (zh) * | 2021-06-04 | 2022-04-01 | 河南大学 | 一种适用于疫苗及抗体高效生产的动物细胞高密度培养系统 |
| CN114107045B (zh) * | 2021-10-12 | 2023-08-11 | 江苏蒙彼利生物科技有限公司 | 一种无血清干细胞培养灌流装置及灌流方法 |
| CN119630470A (zh) * | 2022-07-28 | 2025-03-14 | 株式会社大赛璐 | 包含微小有用物质的液体的纯化浓缩装置、以及使用了该装置的包含微小有用物质的纯化浓缩液的制造方法 |
| AU2023338692A1 (en) * | 2022-09-06 | 2025-02-20 | Amgen Inc. | Large scale bioreactor system and method |
| AU2024294028A1 (en) * | 2023-07-11 | 2026-01-22 | Amgen Inc. | Alternating recirculating tangential flow (artf) filtration for cell culture media perfusion and bioreactor harvesting |
| WO2026019782A2 (en) | 2024-07-16 | 2026-01-22 | Amgen Inc. | Methods for reducing filter fouling in perfusion cell cultures with recirculating tangential flow filtration |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2225789A (en) * | 1986-03-26 | 1990-06-13 | Takeshi Kobayashi | Culture apparatus |
| US6544424B1 (en) * | 1999-12-03 | 2003-04-08 | Refined Technology Company | Fluid filtration system |
| US20110111486A1 (en) * | 2004-01-12 | 2011-05-12 | Mayfair Technology Limited Liability Company d/b/a/ PendoTECH | Bioreactor systems and disposable bioreactor |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2004425A (en) | 1933-04-05 | 1935-06-11 | William H Bain | Shoe and process of making same |
| US6511124B2 (en) | 2001-01-23 | 2003-01-28 | Mark Ellis Combs | Tray table for a child's car seat and associated methods |
| EP1354941A1 (en) | 2002-04-19 | 2003-10-22 | Computer Cell Culture Center S.A. | Apparatus and method for a cell culture in a bioreactor at high cell concentration |
| EP1648586A4 (en) | 2003-06-17 | 2007-09-05 | Centocor Inc | METHOD AND APPARATUS FOR FILTRATION OF RECOMBINANT BIOREACTOR PROTEINS |
| CA2557046A1 (en) | 2004-03-05 | 2005-10-13 | John Crowley | Process for cell culturing by continuous perfusion and alternating tangential flow |
| ES2657055T3 (es) | 2007-08-09 | 2018-03-01 | Wyeth Llc | Uso de perfusión para mejorar la producción de un cultivo de células alimentado por lotes en biorreactores |
| US20130270165A1 (en) | 2010-08-25 | 2013-10-17 | Jerry Shevitz | Fluid Filtration Systems |
| AU2011339328A1 (en) | 2010-12-09 | 2013-07-04 | Toray Industries, Inc. | Method for producing chemical by continuous fermentation |
-
2013
- 2013-09-25 EP EP13840366.2A patent/EP2900804B1/en active Active
- 2013-09-25 JP JP2015534432A patent/JP6321661B2/ja active Active
- 2013-09-25 WO PCT/SE2013/051110 patent/WO2014051503A1/en not_active Ceased
- 2013-09-25 CN CN201811359986.6A patent/CN110066730A/zh active Pending
- 2013-09-25 US US14/430,346 patent/US9663753B2/en active Active
- 2013-09-25 CN CN201380050422.3A patent/CN104640973A/zh active Pending
-
2015
- 2015-02-10 IN IN1067DEN2015 patent/IN2015DN01067A/en unknown
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2225789A (en) * | 1986-03-26 | 1990-06-13 | Takeshi Kobayashi | Culture apparatus |
| US6544424B1 (en) * | 1999-12-03 | 2003-04-08 | Refined Technology Company | Fluid filtration system |
| US20110111486A1 (en) * | 2004-01-12 | 2011-05-12 | Mayfair Technology Limited Liability Company d/b/a/ PendoTECH | Bioreactor systems and disposable bioreactor |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114616044A (zh) * | 2019-10-10 | 2022-06-10 | 伊康斯博尔公司 | 一种具有内置阀门的膜式气体分离器及其打开和关闭方法 |
| CN114616044B (zh) * | 2019-10-10 | 2024-10-18 | 伊康斯博尔公司 | 一种具有内置阀门的膜式气体分离器及其打开和关闭方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP2900804B1 (en) | 2019-09-18 |
| US20150247114A1 (en) | 2015-09-03 |
| JP2015530110A (ja) | 2015-10-15 |
| US9663753B2 (en) | 2017-05-30 |
| IN2015DN01067A (https=) | 2015-06-26 |
| WO2014051503A1 (en) | 2014-04-03 |
| JP6321661B2 (ja) | 2018-05-09 |
| EP2900804A4 (en) | 2015-10-07 |
| CN104640973A (zh) | 2015-05-20 |
| EP2900804A1 (en) | 2015-08-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9663753B2 (en) | Tangential flow perfusion system | |
| US12110484B2 (en) | Disposable bioprocess system supporting biological activity | |
| US10760041B2 (en) | Cell culture bag with internal dialysis membrane | |
| JP5893051B2 (ja) | 交互空気圧膜式細胞分離システム | |
| KR101990651B1 (ko) | 유체 여과 시스템 | |
| KR102718795B1 (ko) | 정유량 펌프/튜빙 시스템이 있는 고정층 바이오 리액터 | |
| US20230056468A1 (en) | Retention system | |
| US20190240622A1 (en) | Single-use process vessel with integrated filter module | |
| US20230042475A1 (en) | Disposable bioprocess system supporting biological activity | |
| CN119499880A (zh) | 一种过滤系统及其方法 | |
| KR20080018161A (ko) | 세포 배양 모듈에 대한 배지 제공 장치 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: uppsala Applicant after: Stoivan Sweden Limited Address before: uppsala Applicant before: GE HEALTHCARE BIO-SCIENCES AB |
|
| CB02 | Change of applicant information |