CN110050872A - A kind of enzyme process prepares the industrialized preparing process of oyster peptide - Google Patents
A kind of enzyme process prepares the industrialized preparing process of oyster peptide Download PDFInfo
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- CN110050872A CN110050872A CN201910384655.6A CN201910384655A CN110050872A CN 110050872 A CN110050872 A CN 110050872A CN 201910384655 A CN201910384655 A CN 201910384655A CN 110050872 A CN110050872 A CN 110050872A
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- 241000237502 Ostreidae Species 0.000 title claims abstract description 145
- 235000020636 oyster Nutrition 0.000 title claims abstract description 145
- 238000000034 method Methods 0.000 title claims abstract description 57
- 230000008569 process Effects 0.000 title claims abstract description 54
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 53
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 22
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 22
- 230000006835 compression Effects 0.000 claims abstract description 22
- 238000007906 compression Methods 0.000 claims abstract description 22
- 235000013372 meat Nutrition 0.000 claims abstract description 16
- 239000000796 flavoring agent Substances 0.000 claims abstract description 15
- 235000019634 flavors Nutrition 0.000 claims abstract description 15
- 239000002781 deodorant agent Substances 0.000 claims abstract description 11
- 238000002203 pretreatment Methods 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims description 50
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 32
- 108091005804 Peptidases Proteins 0.000 claims description 19
- 239000004365 Protease Substances 0.000 claims description 19
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 19
- 239000002002 slurry Substances 0.000 claims description 18
- 239000000835 fiber Substances 0.000 claims description 14
- 230000002255 enzymatic effect Effects 0.000 claims description 12
- 238000000108 ultra-filtration Methods 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 9
- 230000000694 effects Effects 0.000 claims description 9
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 8
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 8
- 229920002521 macromolecule Polymers 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 7
- 238000001728 nano-filtration Methods 0.000 claims description 7
- 239000011347 resin Substances 0.000 claims description 7
- 229920005989 resin Polymers 0.000 claims description 7
- 239000012530 fluid Substances 0.000 claims description 6
- 238000003825 pressing Methods 0.000 claims description 6
- 238000000926 separation method Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 5
- 230000002779 inactivation Effects 0.000 claims description 5
- 238000001179 sorption measurement Methods 0.000 claims description 5
- 229920000049 Carbon (fiber) Polymers 0.000 claims description 4
- 239000004917 carbon fiber Substances 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 4
- 239000004744 fabric Substances 0.000 claims description 4
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 238000001694 spray drying Methods 0.000 claims description 4
- 238000010792 warming Methods 0.000 claims description 4
- 238000004821 distillation Methods 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 238000009210 therapy by ultrasound Methods 0.000 claims description 3
- 230000008719 thickening Effects 0.000 claims description 3
- 230000001351 cycling effect Effects 0.000 claims description 2
- 230000008676 import Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 11
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 abstract description 8
- 239000004615 ingredient Substances 0.000 abstract description 7
- 229920002527 Glycogen Polymers 0.000 abstract description 5
- 229940096919 glycogen Drugs 0.000 abstract description 5
- 239000012535 impurity Substances 0.000 abstract description 5
- 238000010521 absorption reaction Methods 0.000 abstract description 4
- 150000001413 amino acids Chemical class 0.000 abstract description 4
- 238000005265 energy consumption Methods 0.000 abstract description 4
- 235000016709 nutrition Nutrition 0.000 abstract description 4
- SBIBMFFZSBJNJF-UHFFFAOYSA-N selenium;zinc Chemical compound [Se]=[Zn] SBIBMFFZSBJNJF-UHFFFAOYSA-N 0.000 abstract description 4
- 229960003080 taurine Drugs 0.000 abstract description 4
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 3
- 239000000047 product Substances 0.000 description 23
- 238000006243 chemical reaction Methods 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- MVORZMQFXBLMHM-QWRGUYRKSA-N Gly-His-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CC1=CN=CN1 MVORZMQFXBLMHM-QWRGUYRKSA-N 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 108010038983 glycyl-histidyl-lysine Proteins 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 235000019640 taste Nutrition 0.000 description 3
- 108010009736 Protein Hydrolysates Proteins 0.000 description 2
- 235000019658 bitter taste Nutrition 0.000 description 2
- 238000004042 decolorization Methods 0.000 description 2
- 239000012065 filter cake Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000003531 protein hydrolysate Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000002910 solid waste Substances 0.000 description 2
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- JJWSNOOGIUMOEE-UHFFFAOYSA-N Monomethylmercury Chemical compound [Hg]C JJWSNOOGIUMOEE-UHFFFAOYSA-N 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 229910052804 chromium Inorganic materials 0.000 description 1
- 239000011651 chromium Substances 0.000 description 1
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- 230000001427 coherent effect Effects 0.000 description 1
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- 238000009826 distribution Methods 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000003754 machining Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Polymers & Plastics (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Nutrition Science (AREA)
- Health & Medical Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to the industrialized preparing process that a kind of enzyme process prepares oyster peptide, the following steps are included: (1) pre-treatment, (2) it crushes, (3) it digests, (4) it inactivates, (5) it is centrifuged, (6) deodorant decoloration and a plate compression, (7) secondary plate compression, (8) primary purifying concentration, (9) secondarily purified concentration, the concentration of (10) negative pressure, (11) are instantaneously spray-dried.Present invention process is simple, mild condition, easily controllable, and the generation period is short, and yield is high, and low energy consumption, is more suitable for industrialized production；Present invention process oyster peptide produced is based on small-molecular peptides, and molecular weight is small, easy absorption, in addition the nutritional ingredients such as glycogen rich in, free amino acid, taurine and zinc selenium, really realizes the higher value application of oyster meat；It is remained in present invention process oyster peptide produced without other impurities, pure color, excellent in flavor, mouthfeel are prominent, are more liked by consumer.
Description
Technical field
The present invention relates to the industrialized preparing process that a kind of enzyme process prepares oyster peptide, are related to oyster peptide preparation technical field.
Background technique
Oyster is one of big cultivated shellfish in China 4, resourceful.Good protein rich in oyster meat, glycogen,
The health care curative effect that taurine and zinc selenium and other trace elements, referred to as " ocean milk " and China are classified as integration of drinking and medicinal herbs in the first batch
One of food.
At home, oyster mainly with fresh food and it is drying based on, the problems such as industry level of processing is low and product category is few, is increasingly
It highlights.Demand with the majority of consumers to high quality marine food improves, and backward conventional machining techniques have been unable to satisfy people
Therefore how class demand realizes intensive processing and the higher value application of oyster, develop more preferably functional oysters and produce
Product are both the huge opportunity that oyster industry faces and the severe challenge that oyster industry faces.As marine resources utilize
Deepen continuously, the small peptide of marine organisms is prepared from biological enzymolysis technology, because its molecular weight is small, biological value is high,
The advantages such as physiological activity is good, stability is good, safety is portable and receive significant attention.
However oyster Gly-His-Lys on the market cause due to having carried out the processing modes such as high temperature and pressure boiling during the preparation process
Maillard reaction has occurred for itself in raw material, so that it be made to lose flavor and color specific to oyster meat, product quality ichthyosauru
Mix.In addition, oyster Gly-His-Lys on the market may be not thorough due to the enzymatic hydrolysis of biological enzyme during the preparation process or isolate and purify
Reason, the oyster Gly-His-Lys solution caused is muddy, mouthfeel is bad.
The present invention passes through the processing such as optimization enzymatic hydrolysis condition, deodorant decoloration, separating and purifying technology using fresh oyster meat as raw material
Technology prepares a pure flavor, mouthfeel oyster peptide outstanding, and molecular weight is small, easy absorption, really realizes the high level of oyster meat
Change and utilizes.
Summary of the invention
The present invention in view of the deficienciess of the prior art, provide the industrialized preparing process that a kind of enzyme process prepares oyster peptide,
The industrialized preparing process mild condition, easily controllable, obtained oyster peptide pure flavor, molecular weight is small, is easy to absorb, product
Matter is higher.
The technical scheme to solve the above technical problems is that a kind of enzyme process prepares the industrialized production side of oyster peptide
Method, comprising the following steps:
(1) pre-treatment: the meat pure water clean the surface of fresh oyster is taken, dirt is washed off；Fresh oyster meat itself contains rich
The nutritional ingredients such as rich free amino acid, taurine, zinc selenium will lead to a large amount of nutritional ingredient if pretreatment cleaning is improper
It is lost, pretreatment mode of the invention only simply cleans surface with pure water, and dirt is washed off, can not be excessively clear
It washes, it can be ensured that the effective component in oyster meat is prevented from losing, and pre-treatment work is more convenient.
(2) it crushes: the oyster meat cleaned up in step (1) being dripped into lower moisture, is crushed by cutmixer, forms oyster material
Slurry；
(3) it digests: the obtained oyster slurry in step (2) is pumped into enzymatic vessel by pressure pump, pure water is added,
50-60 DEG C of temperature, nature pH in enzymatic vessel are adjusted, is added oyster specific complex protease hydrolyzed 60-90 minutes, adds flavor
Then protease digests 30-40 minutes, entire enzymolysis process keeps ultrasonic treatment to the oyster slurry in enzymatic vessel.Described
Oyster specific complex protease is Angel compound protease MF103 (250000u/g, Angel Yeast Co., Ltd).Enzymatic hydrolysis
In the process without adjusting pH, using nature pH, operating condition is mild, easily controllable, enzyme of the compound protease to Oyster Protein matter
It solves ratio more thoroughly, both facilitates subsequent pressure-filtering process, while greatly improving the yield of product, increase economic benefit；Flavor egg
The addition of white enzyme can improve the bad flavors such as the bitter taste of enzymolysis liquid, to obtain the higher oyster peptide of quality；In addition traditional work
It is needed in skill to oyster meat progress high temperature high pressure process, and oyster meat amino acid rich in itself, protein and glycogen etc.
Ingredient, under high-temperature and high-pressure conditions, part glycogen can be hydrolyzed to glucose, and Maillard reaction can occur for enzymolysis liquid, to influence
The quality of product, and may further ensure that the product quality of oyster peptide in the present invention without high temperature and pressure operation；Ultrasound
The addition of operation can greatly improve enzymolysis efficiency, shorten industrial production campaign.
(4) it inactivates: the oyster slurry after enzymatic hydrolysis in step (3) being heated to 75-80 DEG C, is kept for 5-10 minutes；In 75-80
It is kept for 5-10 minutes at DEG C, it can be ensured that inactivation sufficiently, and can be avoided enzymolysis liquid and Maillard reaction occurs, if temperature is super
Crossing 80 DEG C or time is more than 10min, will lead to enzymolysis liquid itself and Maillard reaction occurs, the color of enzymolysis liquid is made to redden, from
And influence the quality of oyster peptide.
(5) it is centrifuged: the oyster enzymolysis liquid after inactivation in step (4) being cooled to 50-55 DEG C, the strainer through 170-200 mesh
Filtering, then into being centrifuged in centrifuge, centrifugate blowback is into enzymatic vessel；Centrifuge can produce during operation
Oyster enzymolysis liquid is first cooled to 50-55 DEG C by heat, and oyster enzymolysis liquid temperature is excessively high in avoidable centrifugal process, leads to oyster enzyme
It solves liquid and Maillard reaction occurs, in addition the temperature of oyster enzymolysis liquid can not be too low, if temperature is too low, is unfavorable for being centrifugated
Operation.
(6) deodorant decoloration and a plate compression: the attached layer of active carbon fiber on the filter cloth of sheet frame, by oyster enzymolysis liquid
It imported into plate and frame filter press and carries out filters pressing, and the use of AG-3000# diatomite is filter aid.Activated carbon fibre may be implemented to male
The deodorant decolorization of oyster enzymolysis liquid, it can be ensured that obtained oyster peptide color is good-looking, pure flavor, in addition in the filter cloth of sheet frame
Upper attached layer of active carbon fiber may be implemented deodorant decolorization operations and carry out simultaneously with a plate compression operation, shortens production week
Phase improves production efficiency；Traditional active carbon, deodorant good decolorizing effect are substituted using activated carbon fibre, and can repeat to make
With, the generation of a large amount of solid wastes is avoided, industrialized production is more suitable for, it in addition can be to avoid residual activity in final oyster peptide product
Carbon powder influences the quality of product.One time plate compression uses AG-3000# diatomite as filter aid, is because of oyster internal organ
Comparison of ingredients it is complicated, so the oyster enzymolysis liquid ingredient after enzymatic hydrolysis is also more complicated, be easy to block in pressure-filtering process, once
Plate compression using AG-3000# diatomite as filter aid, the transmitance of filter cake compare it is larger, can by some partial sizes compared with
Big impurity filters, and filter efficiency is higher.
(7) secondary plate compression: the oyster enzymolysis liquid after a plate compression is continued to imported into plate and frame filter press progress
Filters pressing uses AG-800# diatomite as filter aid；Oyster enzymolysis liquid after a plate compression still suffers from slightly
Muddiness, using AG-800# diatomite as filter aid, the transmitance of filter cake reduces, so male after secondary plate compression
Oyster enzymolysis liquid clear, avoids the remaining of impurity.
(8) primary purifying concentration: the oyster enzymolysis liquid after secondary plate compression is pumped into fluid reservoir, ultrafiltration is cycled through and sets
Standby, ultra-filtration and separation oyster enzymolysis liquid removes 50% moisture through nanofiltration concentrating and desalinating；
(9) secondarily purified concentration: the oyster enzymolysis liquid after the completion of primary purifying concentration is pumped into fluid reservoir, is cycled through super
Filter equipment, ultra-filtration and separation oyster enzymolysis liquid, through nanofiltration concentrating and desalinating, the moisture of 80% or more removal.Ultrafiltration, can will be compared with
The peptide of macromolecule retains away, to increase the percentage composition of oyster peptide product small molecular peptide；Nanofiltration concentration can be removed
Yan Heshui in oyster enzymolysis liquid.
(10) negative pressure is concentrated: the oyster enzymolysis liquid of secondarily purified concentration being pumped into single effect evaporator, negative pressure concentration, often
30min discharges flush distillation condensed water, after the completion of concentration, is warming up to 70 DEG C or more；Warming temperature is subsequent instantaneous spraying dry
It is dry to be ready, shorten drying time.
(11) instantaneous spray drying: the oyster enzymolysis liquid after negative pressure is concentrated and by heating is pumped into drying tower and is opened
Beginning drying, is instantaneously dried to powder.The instantaneous spray drying time used is very short, can be denaturalized to avoid oyster enzymolysis liquid.
Based on the above technical solution, the present invention can also be improved as follows:
Further, in step (3), the additional amount of the oyster specific complex protease is oyster slurry weight
0.3%；The additional amount of the flavor protease is the 0.1% of oyster slurry weight.In the oyster specific complex protease and wind
In the case where taste protease additional amount, it both may insure that enzymolysis process was complete, and it is too multiple to can be avoided subsequent inactivation process again
It is miscellaneous.
Further, in step (3), in enzymolysis process, stirring in every 10 minutes is primary, can make oyster specific complex albumen
Enzyme and flavor protease are fully utilized, it is ensured that enzymatic hydrolysis is complete.
Further, in step (5), centrifugal process, centrifugation rate 4000r/min.It, both can be under the centrifugation rate
Ensure centrifugal efficiency, and energy consumption can be reduced, is more suitable for industrialized production.
Further, the activated carbon fibre is before use, first carry out microwave irradiation processing for the activated carbon fibre.Make
It is more preferable to the deodorant decolorizing effect of enzymolysis liquid with by microwave irradiation treated activated carbon fibre, to be more conducive to obtain color
Damp pure, the good oyster peptide product of taste.
Further, the activated carbon fibre is replaced after reusing 5-10 times, and activated carbon fibre makes in sheet frame
With being replaced after 5-10 times, the reuse of activated carbon fibre both may be implemented, saved production cost, and can ensure to male
The deodorant decolorizing effect of oyster enzymolysis liquid.
Further, the oyster enzymolysis liquid after secondary plate compression carries out again after first cycling through macromolecule resin adsorption column
Primary purifying concentration, oyster enzymolysis liquid is by can effectively remove the huge sum of money in oyster enzymolysis liquid after macromolecule resin adsorption column
Belong to, it is ensured that content of beary metal is up to standard in obtained oyster peptide product, will not cause harm to the human body.Oyster belongs to seashell
Class tends to, by the processing of macromolecule resin adsorption column, can effectively solve the problems, such as heavy metal containing heavy metal substance,
The macromolecule resin for further ensuring that obtain the oyster peptide product of high-quality, and filling in macromolecule resin adsorption column can weigh
It is multiple to use, it is more suitable for industrialized production theory.
Further, in step (10), the negative pressure of negative pressure concentration is 0.08Mpa, and thickening temperature is 50-70 DEG C, at this
Rapid concentration may be implemented under part, improve production efficiency, reduce energy consumption, and can be to avoid generation Maillard reaction.
Further, in step (11), the temperature being instantaneously spray-dried is 150-160 DEG C, be may be implemented instantaneously dry
It is dry at powder.
The beneficial effects of the present invention are:
(1) present invention process is simple, mild condition, easily controllable, and the generation period is short, and yield is high, and low energy consumption, is more suitable for work
Industry metaplasia produces；
(2) traditional active carbon, deodorant good decolorizing effect are substituted using activated carbon fibre, and may be reused, kept away
Exempt from the generation of a large amount of solid wastes, high production efficiency is more suitable for industrialized production, in addition can be to avoid residual in final oyster peptide product
Active carbon powder is stayed, the quality of product is influenced；
(3) impurity in enzymolysis liquid is sufficiently removed, using the operation of plate compression twice using purifying twice in the present invention
Concentration can sufficiently remove the Yan Heshui in oyster enzymolysis liquid, it is ensured that the quality of final oyster peptide product；
(4) present invention process oyster peptide produced is based on small-molecular peptides, relative molecular mass≤1000u small molecule
Peptide content can reach 94% or more, and molecular weight is small, easy absorption, in addition glycogen rich in, free amino acid, taurine and
The nutritional ingredients such as zinc selenium really realize the higher value application of oyster meat；
(5) it is remained in present invention process oyster peptide produced without other impurities, pure color, excellent in flavor, mouthfeel are prominent
Out, it is good oyster peptide product, and is more liked by consumer.
Detailed description of the invention
Fig. 1 is the chromatogram of oyster peptide product in embodiment.
Specific embodiment
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, below to specific reality of the invention
The mode of applying is described in detail.In the following description, numerous specific details are set forth in order to facilitate a full understanding of the present invention.But
The invention can be embodied in many other ways as described herein, and those skilled in the art can be without prejudice to this hair
Similar improvement is done in the case where bright intension, therefore the present invention is not limited by the specific embodiments disclosed below.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.
A kind of enzyme process prepares the industrialized preparing process of oyster peptide, comprising the following steps:
(1) pre-treatment: the meat pure water clean the surface of fresh oyster is taken, dirt is washed off；
(2) it crushes: the oyster meat cleaned up in step (1) being dripped into lower moisture, is crushed by cutmixer, forms oyster material
Slurry；
(3) it digests: the obtained oyster slurry in step (2) is pumped into enzymatic vessel by pressure pump, pure water is added,
50-60 DEG C of temperature, nature pH in enzymatic vessel are adjusted, is added oyster specific complex protease hydrolyzed 60 minutes, the oyster is dedicated
The additional amount of compound protease is the 0.3% of oyster slurry weight；Flavor protease is added, is then digested 30 minutes, it is described
The additional amount of flavor protease is the 0.1% of oyster slurry weight；In enzymolysis process, stirring in every 10 minutes is primary, entire to digest
Process keeps ultrasonic treatment to the oyster slurry in enzymatic vessel, and the oyster specific complex protease is Angel compound protease
MF103 (250000u/g, Angel Yeast Co., Ltd)；
(4) it inactivates: the oyster slurry after enzymatic hydrolysis in step (3) being heated to 75-80 DEG C, is kept for 5 minutes；
(5) it is centrifuged: the oyster enzymolysis liquid after inactivation in step (4) being cooled to 50-55 DEG C, the strainer through 170-200 mesh
Filtering, then into being centrifuged in centrifuge, centrifugation rate 4000r/min, centrifugate blowback is into enzymatic vessel；
(6) deodorant decoloration and a plate compression: the attached layer of active carbon fiber on the filter cloth of sheet frame, by oyster enzymolysis liquid
Imported into plate and frame filter press carry out filters pressing, and using AG-3000# diatomite be filter aid, the activated carbon fibre before use,
The activated carbon fibre is first subjected to microwave irradiation processing；
(7) secondary plate compression: the oyster enzymolysis liquid after a plate compression is continued to imported into plate and frame filter press progress
Filters pressing is filter aid using AG-800# diatomite；
(8) the oyster enzymolysis liquid after secondary plate compression first primary purifying concentration: is cycled through into macromolecule resin absorption
After column, then it is pumped into fluid reservoir, cycles through ultrafiltration apparatus, ultra-filtration and separation oyster enzymolysis liquid, through nanofiltration concentrating and desalinating, removal 50%
Moisture；
(9) secondarily purified concentration: the oyster enzymolysis liquid after the completion of primary purifying concentration is pumped into fluid reservoir, is cycled through super
Filter equipment, ultra-filtration and separation oyster enzymolysis liquid, through nanofiltration concentrating and desalinating, the moisture of 80% or more removal；
(10) negative pressure is concentrated: the oyster enzymolysis liquid of secondarily purified concentration being pumped into single effect evaporator, negative pressure concentration is born
The negative pressure of pressure concentration is 0.08Mpa, and thickening temperature is 50-70 DEG C, and every 30min discharges flush distillation condensed water, after the completion of concentration,
It is warming up to 70 DEG C or more；
(11) instantaneous spray drying: the oyster enzymolysis liquid after negative pressure is concentrated and by heating is pumped into drying tower and is opened
Beginning drying, the temperature being instantaneously spray-dried are 150-160 DEG C, are instantaneously dried to powder, obtain oyster peptide product.
The recovery rate of the oyster peptide is 78.5%, and the chromatography of the oyster peptide is as shown in Figure 1, oyster peptide molecular weight
Other coherent detection data of distributed data such as the following table 1, oyster peptide are as shown in table 2.
1 oyster peptide molecular weight distribution table of table
Relative molecular mass≤3000u protein hydrolysate accounts for 99.45% in the oyster peptide product, average molecular matter
Amount≤1000u protein hydrolysate accounts for 94.56%, and small-molecular peptides content is higher, it is easier to be absorbed, and the oyster peptide
Product without fishy smell, without bitter taste, more preferably, color is purer for taste.
2 oyster peptide product testing data of table
| Project | Detection data |
| Moisture/% | ≤10.0 |
| Ash content/% | ≤8.0 |
| Inorganic arsenic/(mg/kg) | ≤0.5 |
| Methyl mercury/(mg/kg) | ≤0.5 |
| Lead/(mg/kg) | ≤1.0 |
| Chromium/(mg/kg) | ≤2.0 |
It can be seen from the data in Table 2 that the oyster peptide product items detection data that process through the invention obtains
It meets the requirements of the standard, quality is higher, safer health.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (9)
1. the industrialized preparing process that a kind of enzyme process prepares oyster peptide, which comprises the following steps:
(1) pre-treatment: the meat pure water clean the surface of fresh oyster is taken, dirt is washed off；
(2) it crushes: the oyster meat cleaned up in step (1) being dripped into lower moisture, is crushed by cutmixer, forms oyster slurry；
(3) it digests: the obtained oyster slurry in step (2) is pumped into enzymatic vessel by pressure pump, pure water is added, adjust
50-60 DEG C of temperature, nature pH in enzymatic vessel are added oyster specific complex protease hydrolyzed 60-90 minutes, add flavor albumen
Then enzyme digests 30-40 minutes, entire enzymolysis process keeps ultrasonic treatment to the oyster slurry in enzymatic vessel；
(4) it inactivates: the oyster slurry after enzymatic hydrolysis in step (3) being heated to 75-80 DEG C, is kept for 5-10 minutes；
(5) it is centrifuged: the oyster enzymolysis liquid after inactivation in step (4) is cooled to 50-55 DEG C, the strainer filtering through 170-200 mesh,
Then into being centrifuged in centrifuge, centrifugate blowback is into enzymatic vessel；
(6) deodorant decoloration and a plate compression: the attached layer of active carbon fiber on the filter cloth of sheet frame imports oyster enzymolysis liquid
Filters pressing is carried out to plate and frame filter press, and the use of AG-3000# diatomite is filter aid；
(7) secondary plate compression: the oyster enzymolysis liquid after a plate compression being continued to imported into plate and frame filter press progress filters pressing,
It the use of AG-800# diatomite is filter aid；
(8) primary purifying concentration: being pumped into fluid reservoir for the oyster enzymolysis liquid after secondary plate compression, cycle through ultrafiltration apparatus,
Ultra-filtration and separation oyster enzymolysis liquid removes 50% moisture through nanofiltration concentrating and desalinating；
(9) secondarily purified concentration: the oyster enzymolysis liquid after the completion of primary purifying concentration is pumped into fluid reservoir, ultrafiltration is cycled through and sets
It is standby, ultra-filtration and separation oyster enzymolysis liquid, through nanofiltration concentrating and desalinating, the moisture of 80% or more removal；
(10) negative pressure is concentrated: the oyster enzymolysis liquid of secondarily purified concentration being pumped into single effect evaporator, negative pressure concentration, often
30min discharges flush distillation condensed water, after the completion of concentration, is warming up to 70 DEG C or more；
(11) instantaneous spray drying: the oyster enzymolysis liquid after negative pressure is concentrated and by heating is pumped into drying tower and is hopped to it
It is dry, instantaneously it is dried to powder.
2. the industrialized preparing process that a kind of enzyme process according to claim 1 prepares oyster peptide, which is characterized in that step
(3) in, the additional amount of the oyster specific complex protease is the 0.3% of oyster slurry weight；The flavor protease adds
Enter 0.1% that amount is oyster slurry weight.
3. the industrialized preparing process that a kind of enzyme process according to claim 1 prepares oyster peptide, which is characterized in that step
(3) in, in enzymolysis process, stirring in every 10 minutes is primary.
4. the industrialized preparing process that a kind of enzyme process according to claim 1 prepares oyster peptide, which is characterized in that step
(5) in, centrifugal process, centrifugation rate 4000r/min.
5. the industrialized preparing process that a kind of enzyme process according to claim 1 prepares oyster peptide, which is characterized in that step
(6) in, the activated carbon fibre is before use, first carry out microwave irradiation processing for the activated carbon fibre.
6. the industrialized preparing process that a kind of enzyme process according to claim 5 prepares oyster peptide, which is characterized in that described
Activated carbon fibre is replaced after reusing 5-10 times.
7. the industrialized preparing process that a kind of enzyme process according to claim 1 prepares oyster peptide, which is characterized in that secondary
Oyster enzymolysis liquid after plate compression carries out once purifying concentration again after first cycling through macromolecule resin adsorption column.
8. the industrialized preparing process that a kind of enzyme process according to claim 1 prepares oyster peptide, which is characterized in that step
(10) in, the negative pressure of negative pressure concentration is 0.08Mpa, and thickening temperature is 50-70 DEG C.
9. the industrialized preparing process that a kind of enzyme process according to claim 1 prepares oyster peptide, which is characterized in that step
(11) in, the temperature being instantaneously spray-dried is 150-160 DEG C.
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| PCT/CN2019/097387 WO2020224058A1 (en) | 2019-05-09 | 2019-07-24 | Industrialized production method for preparing oyster peptide by means of enzymatic method |
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