CN103255190A - Method and process for extracting animal micro-molecule polypeptides and amino acids by utilizing complex enzyme - Google Patents
Method and process for extracting animal micro-molecule polypeptides and amino acids by utilizing complex enzyme Download PDFInfo
- Publication number
- CN103255190A CN103255190A CN2013102065877A CN201310206587A CN103255190A CN 103255190 A CN103255190 A CN 103255190A CN 2013102065877 A CN2013102065877 A CN 2013102065877A CN 201310206587 A CN201310206587 A CN 201310206587A CN 103255190 A CN103255190 A CN 103255190A
- Authority
- CN
- China
- Prior art keywords
- enzymolysis
- animal
- prozyme
- enzyme
- temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method and process for extracting animal micro-molecule polypeptides and amino acids by utilizing a complex enzyme. The method comprises the following steps: (1) crushing animal tissues so that the animal tissues can pass through a sieve of 80-200 meshes, and adding water to prepare a suspension with the concentration of 8-30 percent; (2) raising the temperature to be 35-40 DEG C, regulating the pH value to be 8.0-9.0 by using sodium carbonate, adding a complex enzyme I accounting for 0.5-2.0 percent of the animal tissues, performing ultrasonic enzymolysis at 14-16 kHz for 1-2 hours so that the proteins between cells are subjected to full enzymolysis, regulating the pH value to be 6.5-7.5, adding a complex enzyme II accounting for 0.5-3.0 percent of the animal tissues, fully and uniformly stirring, controlling the temperature of the water bath to be 37-45 DEG C, and performing ultrasonic enzymolysis at 15-17 kHz for 1-6 hours; (3) raising the temperature of the enzymolysis solution to be 90-100 DEG C to inactivate the enzyme for 20-30 minutes after the enzymolysis is finished; (4) filtering the solution subjected to enzymolysis through a 0.22mu m microfiltration membrane; and (5) concentrating the filtrate to the relative density of 1:1.0-1:1.2 to prepare the concentrated solution, wherein the complex enzyme II consists of Alcalase, Flavourzyme, Protamex, neutral protease and papain. According to the method, the enzymolysis efficiency can be improved, and the production cost can be reduced.
Description
Technical field
The invention belongs to the protein engineering field, particularly relate to a kind of prozyme that utilizes and extract animal micromolecule polypeptide and amino acid whose method and technology.
Background technology
Compare with plant, the composition of the range protein in the animal body and ratio and people are more approaching, thereby the protein component in the animal body has higher nutritive value to the people, especially some have micromolecule polypeptide and the amino acid (biologically active substance) of particular physiological function in the animal body, and metabolism has important regulatory role to human body.Traditional animal series products mainly adopts physical method (as fragmentation, high temperature etc.) to process, and not only extraction efficiency is low, also can cause the mass efficient composition to destroy loss of biological activity.Although now indivedual courses of processing have adopted biological enzymolysis technology, seriously ignored a problem (choosing of enzyme should be complementary with animal tissues's structure), make the degradation effect of enzyme not ideal enough.Enzyme is produced by the living organisms cell, is subjected to multiple factor to regulate the biological catalyst with catalytic capability of control.Compared its common point with general catalyzer, but outstanding feature (as: the catalytic efficiency height of enzyme has height specificity etc.) has been arranged.The kind of enzyme is a lot, and what be used for the animal enzymolysis mainly is proteolytic enzyme.Proteolytic enzyme is divided into endopeptidase and exopeptidase two classes again, want to take full advantage of the effective constituent in the animal body, just need endopeptidase and exopeptidase combination, with micromolecule polypeptide and the amino acid that abundant degrade proteins macromole utilizes for ease of absorption of human body, this just needs a kind of complex enzyme hydrolysis technology of invention for the protein degradation in the animal body.
Summary of the invention
The invention provides a kind of prozyme that utilizes and extract animal micromolecule polypeptide and amino acid whose method and technology, simple to operate, production cost is low, has improved the utilization ratio of animal protein nutritive ingredient.
The technical solution adopted in the present invention is:
Prozyme is used for extracting animal micromolecule polypeptide and amino acid whose method and concrete production technique:
(1) animal tissues is pulverized, can pass through 80 orders～200 mesh sieve, adding water, to be made into concentration be 8%～30% suspension;
(2) improving temperature is 35 ℃～40 ℃, regulating the pH value with yellow soda ash is 8.0～9.0, the prozyme I that adds animal tissues 0.5%～2.0% again, place it in the Vltrasonic device then, the ultrasonic enzymolysis 1h～2h of 14～16kHz, make the abundant enzymolysis of intercellular albumen, regulating the pH value with yellow soda ash again is 6.5～7.5, add the compound enzyme II that is equivalent to animal tissues 0.5%～3.0%, fully stir evenly, the temperature of control water-bath is at 37 ℃～45 ℃, the ultrasonic enzymolysis 1h～6h of 15～17kHz, in enzymolysis, need suitable stirring water-bath, be conducive to the hydrolysis effect of enzyme like this, shorten enzymolysis time;
(3) after enzymolysis is finished, improve enzymolysis solution temperature to 90 ℃～100 ℃, inactivator 20～30min;
(4) with the solution behind the enzymolysis through 0.22 μ m filtering with microporous membrane;
(5) getting filtrate, to be concentrated into relative density be 1: 1.0～1: 1.2, makes concentrated solution.
Prozyme comprises prozyme I and compound enzyme II.
Prozyme I mainly is made up of trypsinase 30%～70%, steapsase 10%～40% and pancreatic amylase 5%～30%, and the activity unit of enzyme is 20,000 U/g～200,000 U/g, can decompose the protein of intercellular fat and part.
Compound enzyme II is made up of Alcalase (Sumizyme MP) 5%～30%, Flavourzyme (compound protease) 5%～30%, Protamex (compound protease) 5%～30%, neutral protease 10%～50%, papoid 1%～30%, total mass consists of 100%, and the activity unit of enzyme is 50,000 U/g～300,000 U/g.
Advantage of the present invention: utilize enzyme reaction to have highly narrow spectrum characteristics, select endopeptidase and exopeptidase to be mixed in proportion, high molecular weight protein in the animal body is decomposed into micromolecule polypeptide and the amino acid that is conducive to absorption of human body, destruction by cell membrane discharges intracellular effective constituent, can improve enzymolysis efficiency, can reduce production costs again.Can be applicable to the processing of bright sea cucumber, abalone, oyster, pig bone, beef, chicken gizzard, fish-bone, pig's feet, shank, pigskin, ox-hide.
Embodiment
Following examples are used for understanding the present invention better, but are not used for limiting the scope that the present invention protects.
Embodiment 1: utilize prozyme that sea cucumber is carried out enzymolysis.
(1) get bright sea cucumber 1kg, pulverizing can be crossed 80 mesh sieve.
(2) add water and make animal tissues's suspension of 10%, 100 ℃ of heating 30min when temperature is down to 35 ℃～40 ℃, are 8.0 with the adjusting PH with base value, add enteropeptidase, the back adding that stirs is equivalent to the prozyme I of animal tissues 1.0%, and 37 ℃ of constant temperature are hatched, the ultrasonic enzymolysis 1h of 15kHz, adjust pH is 7.0, add the compound enzyme II that is equivalent to animal tissues 1.5%, 45 ℃ of control temperature, the ultrasonic enzymolysis 2h of 16.5kHz.
(3) improve temperature to 100 ℃, quick inactivating enzyme 30min, naturally cooling cooling then.
(4) with the solution behind the enzymolysis through 0.22 μ m filtering with microporous membrane.
(5) getting filtrate, to be concentrated into relative density be 1: 1.0, makes tan concentrated solution.
Embodiment 2: utilize prozyme that bright Oyster is carried out enzymolysis.
(1) get Oyster 1kg, pulverizing can be crossed 100 mesh sieve.
(2) add water and make 25% suspension, improving temperature is 35 ℃～40 ℃, be 8.5 with the yellow soda ash adjust pH, the back adding that stirs is equivalent to the Cotazym I of animal tissue contg 0.8%, and 40 ℃ of constant temperature are hatched, the ultrasonic enzymolysis 1.5h of 14.5kHz, adjust pH is 7.5, add the compound enzyme II that is equivalent to animal tissue contg 1.0%, 40 ℃ of control temperature, the ultrasonic enzymolysis 4h of 15kHz.
(3) improve temperature to 100 ℃, quick inactivating enzyme 20min, naturally cooling cooling then.
(4) with the solution behind the enzymolysis through 0.22 μ m filtering with microporous membrane.
(5) getting filtrate, to be concentrated into relative density be 1: 1.0, makes concentrated solution.
Embodiment 3: utilize prozyme that abalone is carried out enzymolysis.
(1) get abalone meat 1kg, pulverizing can be crossed 100 mesh sieve.
(2) add water and make 20% suspension, when the raising temperature is 35 ℃～40 ℃, be 8.5 with the sodium bicarbonate adjust pH, the back adding that stirs is equivalent to the prozyme I of animal tissue contg 1.1%, and 35 ℃ of constant temperature are hatched, the ultrasonic enzymolysis 1.0h of 16kHz, adjust pH is 6.5, add the compound enzyme II that is equivalent to animal tissue contg 1.2%, temperature is controlled at 37 ℃, the ultrasonic enzymolysis 2h of 17kHz.
(3) improve temperature to 95 ℃, quick inactivating enzyme 30min, naturally cooling cooling then.
(4) with the solution behind the enzymolysis through 0.22 μ m filtering with microporous membrane.
(5) getting filtrate, to be concentrated into relative density be 1: 1.0, makes concentrated solution.
Embodiment 4: utilize prozyme that the pig bone is carried out enzymolysis.
(1) get pig bone 2kg, pulverizing can be crossed 100 mesh sieve.
(2) add water and make 15% suspension, when the raising temperature is 35 ℃～40 ℃, be 8.5 with the yellow soda ash adjust pH, the back adding that stirs is equivalent to the prozyme I of animal tissue contg 1.5%, and 36 ℃ of constant temperature are hatched, the ultrasonic enzymolysis 2.0h of 16kHz, adjust pH is 6.5, add the compound enzyme II that is equivalent to animal tissue contg 2.0%, 42 ℃ of constant temperature are hatched, the ultrasonic enzymolysis 3h of 17kHz.
(3) improve temperature to 90 ℃, quick inactivating enzyme 30min, naturally cooling cooling then.
(4) with the solution behind the enzymolysis through 0.22 μ m filtering with microporous membrane.
(5) getting filtrate, to be concentrated into relative density be 1: 1.2, makes concentrated solution.
Embodiment 5: utilize prozyme that chicken gizzard is carried out enzymolysis.
(1) get chicken gizzard 1kg, pulverizing can be crossed 90 mesh sieve.
(2) add water and make 8% suspension, 80 ℃ of heating 30min, when reducing the temperature to 35 ℃～40 ℃, be 8.0 with the yellow soda ash adjust pH, the back that stirs adds the prozyme I that is equivalent to animal tissue contg 1.2%, 38 ℃ of constant temperature are hatched, the ultrasonic enzymolysis 2.0h of 15kHz, adjust pH is 7.2, adds the compound enzyme II that is equivalent to animal tissue contg 2.5%, 39 ℃ of constant temperature are hatched, the ultrasonic enzymolysis 2h of 16kHz.
(3) improve temperature to 90 ℃, quick inactivating enzyme 30min, naturally cooling cooling then.
(4) with the solution behind the enzymolysis through 0.22 μ m filtering with microporous membrane.
(5) getting filtrate, to be concentrated into relative density be 1: 1.0, makes concentrated solution.
Embodiment 6: utilize prozyme that fish-bone is carried out enzymolysis.
(1) get fish-bone 1kg, pulverizing can be crossed 100 mesh sieves.
(2) add water and make 30% suspension, during rising temperature to 35 ℃～40 ℃, be 8.5 with the adjusting PH with base value, the back adding that stirs is equivalent to the prozyme I of animal tissue contg 1.0%, and 37 ℃ of constant temperature are hatched, the ultrasonic enzymolysis 2.0h of 15kHz, adjust pH is 6.5, add the compound enzyme II that is equivalent to animal tissue contg 0.5%, 55 ℃ of constant temperature are hatched, the ultrasonic enzymolysis 5h of 17kHz.
(3) improve temperature to 100 ℃, quick inactivating enzyme 25min, naturally cooling cooling then.
(4) with the solution behind the enzymolysis through 0.22 μ m filtering with microporous membrane.
(5) getting filtrate, to be concentrated into relative density be 1: 1.1, makes concentrated solution.
Embodiment 7: utilize prozyme that beef is carried out enzymolysis.
(1) get beef 1kg, pulverizing can be crossed 80 mesh sieves.
(2) add water and make 20% suspension, when improving temperature to 35 ℃～40 ℃, be 9.0 with the yellow soda ash adjust pH, the back adding that stirs is equivalent to the prozyme I of animal tissue contg 0.5%, and 35 ℃ of constant temperature are hatched, the ultrasonic enzymolysis 1.5h of 14kHz, adjust pH is 7.0, add the compound enzyme II that is equivalent to animal tissue contg 3.0%, 45 ℃ of constant temperature are hatched, the ultrasonic enzymolysis 3h of 15kHz.
(3) improve temperature to 100 ℃, quick inactivating enzyme 20min, naturally cooling cooling then.
(4) with the solution behind the enzymolysis through 0.22 μ m filtering with microporous membrane.
(5) getting filtrate, to be concentrated into relative density be 1: 1.05, makes concentrated solution.
Claims (4)
1. utilize prozyme to extract animal micromolecule polypeptide and amino acid whose method and technology, it is characterized in that prozyme is used to extract animal micromolecule polypeptide and amino acid whose method and concrete production technique and is: (1) pulverizes animal tissues, can pass through 80 orders～200 mesh sieve, adding water, to be made into concentration be 8%～30% suspension; (2) improving temperature is 35 ℃～40 ℃, regulating the pH value with yellow soda ash is 8.0～9.0, the prozyme I that adds animal tissues 0.5%～2.0% again, place it in the Vltrasonic device then, the ultrasonic enzymolysis 1h～2h of 14～16kHz, make the abundant enzymolysis of intercellular albumen, regulating the pH value with yellow soda ash again is 6.5～7.5, add the compound enzyme II that is equivalent to animal tissues 0.5%～3.0%, fully stir evenly, the temperature of control water-bath is at 37 ℃～45 ℃, the ultrasonic enzymolysis 1h～6h of 15～17kHz, in enzymolysis, need suitable stirring water-bath, be conducive to the hydrolysis effect of enzyme like this, shorten enzymolysis time; (3) after enzymolysis is finished, improve enzymolysis solution temperature to 90 ℃～100 ℃, inactivator 20～30min; (4) with the solution behind the enzymolysis through 0.22 μ m filtering with microporous membrane; (5) getting filtrate, to be concentrated into relative density be 1: 1.0～1: 1.2, makes concentrated solution.
2. the prozyme that utilizes according to claim 1 extracts animal micromolecule polypeptide and amino acid whose method and technology, it is characterized in that prozyme I mainly is made up of trypsinase 30%～60%, steapsase 10%～30% and pancreatic amylase 5%～20%, the activity unit of enzyme is 20,000 U/g～200,000 U/g, can decompose the protein of intercellular fat and part.
3. the prozyme that utilizes according to claim 1 extracts animal micromolecule polypeptide and amino acid whose method and technology, it is characterized in that compound enzyme II is made up of Sumizyme MP 5%～30%, compound protease 5%～30%, compound protease 5%～30%, neutral protease 10%～50%, papoid 1%～30%, total mass consists of 100%, and the activity unit of enzyme is 50,000 U/g～300,000 U/g.
4. the prozyme that utilizes according to claim 1 extracts animal micromolecule polypeptide and amino acid whose method and technology, it is characterized in that can be applicable to the processing of bright sea cucumber, abalone, oyster, pig bone, beef, chicken gizzard, fish-bone, pig's feet, shank, pigskin, ox-hide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102065877A CN103255190A (en) | 2013-05-30 | 2013-05-30 | Method and process for extracting animal micro-molecule polypeptides and amino acids by utilizing complex enzyme |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102065877A CN103255190A (en) | 2013-05-30 | 2013-05-30 | Method and process for extracting animal micro-molecule polypeptides and amino acids by utilizing complex enzyme |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103255190A true CN103255190A (en) | 2013-08-21 |
Family
ID=48959398
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013102065877A Pending CN103255190A (en) | 2013-05-30 | 2013-05-30 | Method and process for extracting animal micro-molecule polypeptides and amino acids by utilizing complex enzyme |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103255190A (en) |
Cited By (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103553749A (en) * | 2013-11-11 | 2014-02-05 | 李世泰 | Method for producing amino acid agricultural fertilizer through compound enzyme hydrolysis of fish skins and fish scales |
| CN103932267A (en) * | 2014-04-30 | 2014-07-23 | 徐海菊 | Processing technique for preparing oyster meat nutritious powder by enzymolysis with assistance of ultrasound |
| CN103947819A (en) * | 2014-04-30 | 2014-07-30 | 桂林军供生化技术开发有限公司 | Method for extracting protein from pigskin |
| CN104068423A (en) * | 2014-06-12 | 2014-10-01 | 渤海大学 | Oyster meat nutrition powder and preparation method thereof |
| CN104621338A (en) * | 2015-03-03 | 2015-05-20 | 浙江小二黑食品有限公司 | Method for preparing novel silkie bone polypeptides |
| CN104805164A (en) * | 2015-04-30 | 2015-07-29 | 中国食品发酵工业研究院 | Preparation method of high protein oyster active peptides with low sensitization |
| CN104894198A (en) * | 2015-04-30 | 2015-09-09 | 中国食品发酵工业研究院 | Preparation method of hypoallergenic total-nutrient oyster active peptide |
| CN104974223A (en) * | 2015-05-12 | 2015-10-14 | 舟山拓智科技服务有限公司 | Actinopyga mauritiana polypeptides and application thereof |
| CN105107815A (en) * | 2015-06-25 | 2015-12-02 | 河南农业大学 | Method for treating illness-dead livestock and poultry by combining chemical method with biological method |
| CN105200105A (en) * | 2015-09-10 | 2015-12-30 | 烟台源力德海洋生物有限公司 | Preparation method of oyster protein liver protecting peptide preparation |
| CN106922954A (en) * | 2015-12-31 | 2017-07-07 | 武汉新华扬生物股份有限公司 | A kind of method for preparing chicken feet hide collagen raw material |
| CN107467344A (en) * | 2017-09-20 | 2017-12-15 | 杨智 | The biologically active peptide and its extracting method extracted in a kind of Phasianidae animal from Galliformes |
| CN107475339A (en) * | 2017-09-20 | 2017-12-15 | 广东雅道生物科技有限公司 | A kind of meat polypeptide extracting method |
| CN107518307A (en) * | 2017-08-02 | 2017-12-29 | 安徽全康药业有限公司 | A kind of pregnant woman, the preparation method of wet nurse's type multivitamin several mineral materials piece |
| CN107821727A (en) * | 2017-10-31 | 2018-03-23 | 宁波海博士特医食品科技有限公司 | A kind of processing method of sea cucumber Gly-His-Lys |
| CN107912768A (en) * | 2017-12-22 | 2018-04-17 | 东阿阿胶股份有限公司 | One breeding ass intacellin and preparation method thereof |
| CN108486087A (en) * | 2018-04-10 | 2018-09-04 | 吴广兵 | It is a kind of enzymolysis chicken gizzard complex enzyme formulation and its application |
| CN108771244A (en) * | 2018-05-24 | 2018-11-09 | 南京中生生物科技有限公司 | The preparation method of the oligomeric peptide extract of abalone, method, abalone oligopeptide and its application for preparing abalone oligopeptide and abalone powder |
| CN108977424A (en) * | 2018-08-02 | 2018-12-11 | 山东隆科特酶制剂有限公司 | A kind of catabolic enzyme preparation of the molten slurry of anchovy fish and its application |
| CN109022400A (en) * | 2018-08-20 | 2018-12-18 | 山东隆科特酶制剂有限公司 | A kind of chicken gizzard degradation complex enzyme preparation special and application |
| CN109170785A (en) * | 2018-09-28 | 2019-01-11 | 北京工商大学 | A method of thermal processing meat flavour is prepared using chicken feet |
| CN109371089A (en) * | 2018-12-25 | 2019-02-22 | 河北肽都生物科技有限公司 | A kind of extracting method of small molecule liver peptide |
| CN109468357A (en) * | 2018-12-25 | 2019-03-15 | 河北肽都生物科技有限公司 | A kind of preparation method of spleen aminopeptide |
| CN109527552A (en) * | 2018-12-21 | 2019-03-29 | 江苏食品药品职业技术学院 | Nutritious food of mid-aged population and preparation method thereof |
| CN110477402A (en) * | 2019-08-09 | 2019-11-22 | 质每堂(武汉)健康科技有限公司 | Fish peptide, fish-bone peptide, sea cucumber peptide and ginseng peptide compound nutritional product and preparation method thereof |
| CN111850064A (en) * | 2020-07-28 | 2020-10-30 | 宁夏顺宝现代农业股份有限公司 | Method for preparing amino acid aqueous solution by using dead chicken |
| WO2020224058A1 (en) * | 2019-05-09 | 2020-11-12 | 烟台嘉惠海洋生物科技有限公司 | Industrialized production method for preparing oyster peptide by means of enzymatic method |
| CN112573961A (en) * | 2020-12-26 | 2021-03-30 | 南宁东恒华道生物科技有限责任公司 | Liquid micro-peptide fertilizer produced by composite enzymolysis of urban fresh garbage, production process and application thereof |
| CN113349308A (en) * | 2021-05-10 | 2021-09-07 | 广州清科生物技术有限公司 | Composite zymolyte for pet food and preparation process thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101263861A (en) * | 2008-05-08 | 2008-09-17 | 广西广明药业有限公司 | Preparation animal protolysate |
| CN102827911A (en) * | 2012-09-24 | 2012-12-19 | 平凉市华科生物技术有限公司 | Preparation method of animal peptone by double-enzyme enzymolysis |
-
2013
- 2013-05-30 CN CN2013102065877A patent/CN103255190A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101263861A (en) * | 2008-05-08 | 2008-09-17 | 广西广明药业有限公司 | Preparation animal protolysate |
| CN102827911A (en) * | 2012-09-24 | 2012-12-19 | 平凉市华科生物技术有限公司 | Preparation method of animal peptone by double-enzyme enzymolysis |
Cited By (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103553749A (en) * | 2013-11-11 | 2014-02-05 | 李世泰 | Method for producing amino acid agricultural fertilizer through compound enzyme hydrolysis of fish skins and fish scales |
| CN103553749B (en) * | 2013-11-11 | 2015-01-14 | 李世泰 | Method for producing amino acid agricultural fertilizer through compound enzyme hydrolysis of fish skins and fish scales |
| CN103932267A (en) * | 2014-04-30 | 2014-07-23 | 徐海菊 | Processing technique for preparing oyster meat nutritious powder by enzymolysis with assistance of ultrasound |
| CN103947819A (en) * | 2014-04-30 | 2014-07-30 | 桂林军供生化技术开发有限公司 | Method for extracting protein from pigskin |
| CN104068423A (en) * | 2014-06-12 | 2014-10-01 | 渤海大学 | Oyster meat nutrition powder and preparation method thereof |
| CN104621338A (en) * | 2015-03-03 | 2015-05-20 | 浙江小二黑食品有限公司 | Method for preparing novel silkie bone polypeptides |
| CN104805164A (en) * | 2015-04-30 | 2015-07-29 | 中国食品发酵工业研究院 | Preparation method of high protein oyster active peptides with low sensitization |
| CN104894198A (en) * | 2015-04-30 | 2015-09-09 | 中国食品发酵工业研究院 | Preparation method of hypoallergenic total-nutrient oyster active peptide |
| CN104894198B (en) * | 2015-04-30 | 2018-07-10 | 中国食品发酵工业研究院 | A kind of full nutrition oyster active peptides preparation method with low irritability |
| CN104974223A (en) * | 2015-05-12 | 2015-10-14 | 舟山拓智科技服务有限公司 | Actinopyga mauritiana polypeptides and application thereof |
| CN105107815A (en) * | 2015-06-25 | 2015-12-02 | 河南农业大学 | Method for treating illness-dead livestock and poultry by combining chemical method with biological method |
| CN105200105A (en) * | 2015-09-10 | 2015-12-30 | 烟台源力德海洋生物有限公司 | Preparation method of oyster protein liver protecting peptide preparation |
| CN106922954A (en) * | 2015-12-31 | 2017-07-07 | 武汉新华扬生物股份有限公司 | A kind of method for preparing chicken feet hide collagen raw material |
| CN107518307A (en) * | 2017-08-02 | 2017-12-29 | 安徽全康药业有限公司 | A kind of pregnant woman, the preparation method of wet nurse's type multivitamin several mineral materials piece |
| CN107467344A (en) * | 2017-09-20 | 2017-12-15 | 杨智 | The biologically active peptide and its extracting method extracted in a kind of Phasianidae animal from Galliformes |
| CN107475339A (en) * | 2017-09-20 | 2017-12-15 | 广东雅道生物科技有限公司 | A kind of meat polypeptide extracting method |
| CN107821727A (en) * | 2017-10-31 | 2018-03-23 | 宁波海博士特医食品科技有限公司 | A kind of processing method of sea cucumber Gly-His-Lys |
| CN107912768A (en) * | 2017-12-22 | 2018-04-17 | 东阿阿胶股份有限公司 | One breeding ass intacellin and preparation method thereof |
| CN107912768B (en) * | 2017-12-22 | 2020-12-11 | 东阿阿胶股份有限公司 | Donkey placenta extract and preparation method thereof |
| CN108486087B (en) * | 2018-04-10 | 2020-11-10 | 吴广兵 | Complex enzyme preparation for enzymolysis of chicken liver and application thereof |
| CN108486087A (en) * | 2018-04-10 | 2018-09-04 | 吴广兵 | It is a kind of enzymolysis chicken gizzard complex enzyme formulation and its application |
| CN108771244A (en) * | 2018-05-24 | 2018-11-09 | 南京中生生物科技有限公司 | The preparation method of the oligomeric peptide extract of abalone, method, abalone oligopeptide and its application for preparing abalone oligopeptide and abalone powder |
| CN108977424A (en) * | 2018-08-02 | 2018-12-11 | 山东隆科特酶制剂有限公司 | A kind of catabolic enzyme preparation of the molten slurry of anchovy fish and its application |
| CN109022400A (en) * | 2018-08-20 | 2018-12-18 | 山东隆科特酶制剂有限公司 | A kind of chicken gizzard degradation complex enzyme preparation special and application |
| CN109170785A (en) * | 2018-09-28 | 2019-01-11 | 北京工商大学 | A method of thermal processing meat flavour is prepared using chicken feet |
| CN109527552A (en) * | 2018-12-21 | 2019-03-29 | 江苏食品药品职业技术学院 | Nutritious food of mid-aged population and preparation method thereof |
| CN109468357A (en) * | 2018-12-25 | 2019-03-15 | 河北肽都生物科技有限公司 | A kind of preparation method of spleen aminopeptide |
| CN109371089A (en) * | 2018-12-25 | 2019-02-22 | 河北肽都生物科技有限公司 | A kind of extracting method of small molecule liver peptide |
| WO2020224058A1 (en) * | 2019-05-09 | 2020-11-12 | 烟台嘉惠海洋生物科技有限公司 | Industrialized production method for preparing oyster peptide by means of enzymatic method |
| CN110477402A (en) * | 2019-08-09 | 2019-11-22 | 质每堂(武汉)健康科技有限公司 | Fish peptide, fish-bone peptide, sea cucumber peptide and ginseng peptide compound nutritional product and preparation method thereof |
| CN111850064A (en) * | 2020-07-28 | 2020-10-30 | 宁夏顺宝现代农业股份有限公司 | Method for preparing amino acid aqueous solution by using dead chicken |
| CN112573961A (en) * | 2020-12-26 | 2021-03-30 | 南宁东恒华道生物科技有限责任公司 | Liquid micro-peptide fertilizer produced by composite enzymolysis of urban fresh garbage, production process and application thereof |
| CN113349308A (en) * | 2021-05-10 | 2021-09-07 | 广州清科生物技术有限公司 | Composite zymolyte for pet food and preparation process thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103255190A (en) | Method and process for extracting animal micro-molecule polypeptides and amino acids by utilizing complex enzyme | |
| CN100489016C (en) | Method of extracting collagen and method of using collagen to prepare collagen protein | |
| CN106011208A (en) | Method for preparing small-molecular weight collagen active peptide through enzymolysis of yak bone and skin | |
| CN101418328A (en) | Method for producing fish scale collagen protein | |
| CN104126807B (en) | Method for continuously producing composite amino acid short peptide chelated calcium and chitin by using waste catering shrimp shells | |
| US20240102071A1 (en) | Method for preparing active protein peptide and prepared active protein peptide and use thereof | |
| CN104789630B (en) | A kind of bluefin tuna ossein hydrolysate and preparation method thereof | |
| CN100569091C (en) | A kind of preparation method of collagen of red fish skin in deep sea | |
| CN103789381A (en) | Method for rapidly extracting fish collagen | |
| CN103609832A (en) | Andrias activity protein powder and preparation method thereof | |
| CN107157797A (en) | A kind of preparation technology of Application of Collagen from Fish Scales facial mask | |
| CN110663957A (en) | Method for extracting calcium-supplementing nutrient substances from yak bones | |
| CN102229971A (en) | Method for preparing collagen peptides by using fresh yak bones | |
| CN110283866B (en) | Preparation method of crucian anti-fatigue active peptide | |
| CN112608968B (en) | Method for producing fish collagen peptide by using tilapia mossambica scale as raw material | |
| KR20170065851A (en) | Efficient manufacturing method of mineral complex using natural ores and clays | |
| CN106282283A (en) | A kind of microwave-assisted prepares the method for freshwater fish bones protein polypeptide | |
| CN107912768B (en) | Donkey placenta extract and preparation method thereof | |
| CN107043800A (en) | A kind of preparation method of collagen | |
| CN107488696B (en) | Chicken bone double-enzymolysis method | |
| CN112724245A (en) | Method for extracting micromolecular collagen peptide by fish scale enzymolysis | |
| CN113151385A (en) | Method for efficiently preparing livestock and poultry cartilage collagen polypeptide | |
| CN114634961B (en) | Preparation method of oyster oligopeptide powder with multiple bioactivity functions | |
| CN115141869A (en) | Preparation method of egg white protein polypeptide with anti-aging and repairing activity | |
| CN105087727A (en) | Method for preparing squid liver protein ACE (angiotensin converting enzyme) inhibitory peptide by enzymolysis of mixed enzyme |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20130821 |