CN110036812A - A kind of method of fructus lycii Arbuscular Mycorrhizal Fungi spore - Google Patents
A kind of method of fructus lycii Arbuscular Mycorrhizal Fungi spore Download PDFInfo
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- CN110036812A CN110036812A CN201910285552.4A CN201910285552A CN110036812A CN 110036812 A CN110036812 A CN 110036812A CN 201910285552 A CN201910285552 A CN 201910285552A CN 110036812 A CN110036812 A CN 110036812A
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- fructus lycii
- mycorrhizal fungi
- arbuscular mycorrhizal
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/10—Mycorrhiza; Mycorrhizal associations
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Life Sciences & Earth Sciences (AREA)
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- Biodiversity & Conservation Biology (AREA)
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- Forests & Forestry (AREA)
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
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- Microbiology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of methods of fructus lycii Arbuscular Mycorrhizal Fungi spore, including preparation method and fructus lycii Arbuscular Mycorrhizal Fungi method including bush mycorrhizal fungi preparation.Method of the invention is to expand numerous arbuscular mycorrhizal fungi by First Year enrichment, the fructus lycii cuttage seeding of root is urged in second year cuttage in the arbuscular mycorrhizal fungi matrix of enrichment, the nutrition root system of arbuscular mycorrhizal fungi and host plant in matrix forms an association, arbuscular mycorrhizal fungi obtains necessary carbohydrate and other nutriments out of fructus lycii seedling body, and fructus lycii seedling also obtains required nutrition and moisture from arbuscular mycorrhizal fungi, to reach a kind of relationship of mutually beneficial mutual assistance, convenient for the growth of fructus lycii seedling, the method of the present invention is compared with other mycorrhizal fungis infect cultural method simultaneously, it is high with root invasion rate, seeded process is easy, the more vigorous advantage of fructus lycii seedling growth.
Description
Technical field
The present invention relates to mycorrhizal fungis to infect fructus lycii related fields more particularly to a kind of fructus lycii Arbuscular Mycorrhizal Fungi spore
The method of son.
Background technique
Arbuscular mycorrhizal fungi has extensive infectivity to plant, it is verified that arbuscular mycorrhizal fungi energy on many plants
Promote host plant to N, P, K, etc. mineral elements absorption, promote plant growth, improve transplanting survival rate of nursery stocks, enhance plant
Disease resistance and for arid, cold, saline and alkaline resistance, can using Mycorrhizal Technology Arbuscular Mycorrhizal Fungi fungi in the forest seedling growth stage
It dramatically increases plant seedlings survival rate, improves seedling quality, there is that seedling stage is short, tree growth ability is strong, wood using Va Mycorrhiza Seedling afforestation
The features such as material yield is high, compared with traditional afforestation technique, mycorrhizal seedling raising afforestation more efficiently, it is low consumption, simple, easily
Row.
When traditional fructus lycii seedling is cultivated, the survival rate of fructus lycii seedling is low, consumption is big, the process of plantation fructus lycii seedling is more complicated,
It is not easy to carry out.
For this purpose, we have proposed a kind of methods of fructus lycii Arbuscular Mycorrhizal Fungi spore.
Summary of the invention
The purpose of the present invention is to provide a kind of methods of fructus lycii Arbuscular Mycorrhizal Fungi spore, to solve above-mentioned background
The problem of being proposed in technology.
To achieve the goals above, present invention employs following technical solutions:
A kind of method of fructus lycii Arbuscular Mycorrhizal Fungi spore, preparation method and Chinese holly including bush mycorrhizal fungi preparation
Qi Arbuscular Mycorrhizal Fungi method, the preparation method of the bush mycorrhizal fungi preparation the following steps are included:
S1: digging pit in vacant lot, and simple-arranged discharge outlet, and smooth good soil is spread in the bottom in the hole dug and surrounding
Greenhouse PO film, for preventing from mixing with other miscellaneous bacterias in soil；
S2: the soil to sterilize after sieving, clean river sand, perlite, the vermiculite that partial size is 2mm are uniformly mixed, filling to digging
At the half cheated well；
S3: bush mycorrhizal fungi preparation is uniformly sprinkled in the filled matrix of half, with a thickness of 1cm or so, so
The matrix mixed is refilled on microbial inoculum afterwards, mixed matrix is smooth, mulch is spread above；
S4: selection host plant, the seed of program request host plant on mulch, with the native earth backing to sterilize after sieving, with true
The seed for protecting host plant can smoothly germinate；
S5: in host plant growth period, nutrient solution is irrigate to irrigating plant, is needed when pouring once within every 30 days, and irrigating
Irrigation volume is controlled；
S6: allowing host plant to thirst naturally after 120 light cultures, then removes plant plantation layer, takes lower layer
Matrix at 10cm detects the content of arbuscular mycorrhizal fungi spore；
The fructus lycii Arbuscular Mycorrhizal Fungi method the following steps are included:
S1: with ready small wooden, upper hole is stabbed in cultured matrix in advance, being broken when for preventing cuttage fructus lycii
The young root that bad fructus lycii is expedited the emergence of out；
S2: plugging the fructus lycii cuttage seeding that root is urged in sand bed, tamps the gap around fructus lycii seedling, cuttage with clean river sand
When pay attention to staying in the bud of cutting outside matrix, cutting exposes best 2cm of matrix or so and is advisable, and spacing in the rows is generally 40*60cm；
S3: it during fructus lycii growth of cuttage seedlings, to pour water, intertill in time, weeding, bud picking.
Preferably, the length and width of the hole depth 25-30cm in the S1, the hole are determined according to the amount of inoculation microbial inoculum.
Preferably, the soil, partial size of the sterilizing are 2mm clean river sand, perlite, vermiculite ratio be 2:3:3:3.
Preferably, the nutrient solution is Gram nutrient solution suddenly, and the concentration of the nutrient solution of Gram suddenly is 1%.
Preferably, the seed of the host plant includes corn seed, alfalfa seed and clover seed, is broadcast in the S4
The seed that kind chooses two kinds of host plants therein is sowed.
Preferably, the growth temperature of the arbuscular mycorrhizal fungi is room temperature.
It preferably, is mild stress to the standard that host plant is irrigated in the S5.
Compared with prior art, the beneficial effects of the present invention are:
Method of the invention is to expand numerous arbuscular mycorrhizal fungi by First Year enrichment, and second year is true in the arbuscular mycorrhiza of enrichment
The fructus lycii cuttage seeding of root is urged in cuttage in bacterium matrix, and the nutrition root system of arbuscular mycorrhizal fungi and host plant in matrix forms one
Association, arbuscular mycorrhizal fungi obtain necessary carbohydrate and other nutriments out of fructus lycii seedling body, and fructus lycii seedling
Required nutrition and moisture is obtained from arbuscular mycorrhizal fungi, to reach a kind of relationship of mutually beneficial mutual assistance, convenient for fructus lycii seedling
Growth, while the method for the present invention has root invasion rate high, seeded process is simple compared with other mycorrhizal fungis infect cultural method
Just, the more vigorous advantage of fructus lycii seedling growth.
Detailed description of the invention
Fig. 1 is a kind of step flow chart of the method for fructus lycii Arbuscular Mycorrhizal Fungi spore proposed by the present invention.
Specific embodiment
Combined with specific embodiments below the present invention is made further to explain, purpose, which is only that, to be better understood in the present invention
Hold.
The invention also provides the preparation methods of a kind of method of fructus lycii Arbuscular Mycorrhizal Fungi spore, including clump branch bacterium
The preparation method and fructus lycii Arbuscular Mycorrhizal Fungi method of mycorrhiza fungi microbial inoculum, the preparation method of the bush mycorrhizal fungi preparation
The following steps are included:
S1: digging pit in vacant lot, and simple-arranged discharge outlet, and smooth good soil is spread in the bottom in the hole dug and surrounding
Greenhouse PO film cheats depth 25-30cm for preventing from mixing with other miscellaneous bacterias in soil, the length and width in hole according to the amount for being inoculated with microbial inoculum come
It determines；
S2: the soil to sterilize after sieving, clean river sand, perlite, the vermiculite that partial size is 2mm are uniformly mixed, filling to digging
At the half cheated well, in order to preferably cultivate bush mycorrhizal fungi preparation, the soil of sterilizing, the clean river that partial size is 2mm
Sand, perlite, vermiculite ratio be 2:3:3:3；
S3: bush mycorrhizal fungi preparation, bush mycorrhizal fungi preparation are uniformly sprinkled in the filled matrix of half
With a thickness of 1cm or so, the matrix mixed in S2 is then refilled on bush mycorrhizal fungi preparation, by mixed matrix
Surface progress is smooth, and mulch is spread on matrix；
S4: the seed of any two kinds of host plants in selection corn seed, alfalfa seed and three kinds of clover seed,
The seed of program request host plant on mulch, with the native earth backing to sterilize after sieving, to ensure that the seed of host plant can smoothly be sent out
Bud；
S5: in host plant growth period, irrigating Gram nutrient solution suddenly to irrigating plant, and Gram nutrient solution is used certainly suddenly
Water is diluted, and the concentration of the nutrient solution of Gram suddenly after dilution is 1%, and Gram nutrient solution concentration too low will lead to is posted suddenly
Main plant is childlike, and root long is little, so influencing the growth of spore, Gram nutrient solution concentration is too high suddenly will lead to host plant
The case where causing burn seedlings generation, and poured according to the actual situation every about 30 days it is primary, and while irrigating need to irrigation volume into
Row control, irrigation norm has three normal irrigation, mild stress and Extreme drought standards, wherein being always maintained at the filling of mild stress
It is best to irrigate standard effect, is learnt according to the prior art, stress energy growth-promoting host plant generates more arbuscular mycorrhizal fungi；
S6: allowing host plant to thirst naturally after 120 light cultures, then removes plant plantation layer, takes lower layer
Matrix at 10cm detects the content of arbuscular mycorrhizal fungi spore；
The fructus lycii Arbuscular Mycorrhizal Fungi method the following steps are included:
S1: with ready small wooden, upper hole is stabbed in cultured matrix in advance, being broken when for preventing cuttage fructus lycii
The young root that bad fructus lycii is expedited the emergence of out；
S2: plugging the fructus lycii cuttage seeding that root is urged in sand bed, tamps the gap around fructus lycii seedling, cuttage with clean river sand
When pay attention to staying in the bud of cutting outside matrix, cutting exposes best 2cm of matrix or so and is advisable, and spacing in the rows is generally 40*60cm；
S3: it during fructus lycii growth of cuttage seedlings, according to the actual conditions that weather condition and fructus lycii seedling are grown, is filled in time
Water, intertillage, weeding, bud picking guarantee the vigorous growth of fructus lycii seedling.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (7)
1. a kind of method of fructus lycii Arbuscular Mycorrhizal Fungi spore, preparation method and fructus lycii including bush mycorrhizal fungi preparation
Arbuscular Mycorrhizal Fungi method, which is characterized in that the preparation method of the bush mycorrhizal fungi preparation the following steps are included:
S1: digging pit in vacant lot, and simple-arranged discharge outlet, and greenhouse is spread in the bottom in the hole dug and surrounding in smooth good soil
PO film, for preventing from mixing with other miscellaneous bacterias in soil；
S2: the soil to sterilize after sieving, clean river sand, perlite, the vermiculite that partial size is 2mm are uniformly mixed, filling to the good hole of digging
Half at；
S3: bush mycorrhizal fungi preparation is uniformly sprinkled in the filled matrix of half, with a thickness of 1cm or so, is then existed
The matrix mixed is refilled above microbial inoculum, mixed matrix is smooth, mulch is spread above；
S4: selection host plant, the seed of program request host plant on mulch, with the native earth backing to sterilize after sieving, to ensure to post
The seed of main plant can smoothly germinate；
S5: in host plant growth period, nutrient solution is irrigate to irrigating plant, is needed when pouring once within every 30 days, and irrigating to filling
The amount of irrigating is controlled；
S6: allowing host plant to thirst naturally after 120 light cultures, then removes plant plantation layer, takes at lower layer 10cm
Matrix, detect arbuscular mycorrhizal fungi spore content；
The fructus lycii Arbuscular Mycorrhizal Fungi method the following steps are included:
S1: with ready small wooden, upper hole is stabbed, in cultured matrix in advance for destroying Chinese holly when preventing cuttage fructus lycii
The young root that Qi expedites the emergence of out；
S2: plugging the fructus lycii cuttage seeding that root is urged in sand bed, tamps the gap around fructus lycii seedling with clean river sand, and when cuttage infuses
Meaning stays in the bud of cutting outside matrix, and cutting exposes best 2cm of matrix or so and is advisable, and spacing in the rows is generally 40*60cm；
S3: it during fructus lycii growth of cuttage seedlings, to pour water, intertill in time, weeding, bud picking.
2. a kind of method of fructus lycii Arbuscular Mycorrhizal Fungi spore according to claim 1, which is characterized in that the S1
In hole depth 25-30cm, the length and width in the hole determines according to the amount of inoculation microbial inoculum.
3. a kind of method of fructus lycii Arbuscular Mycorrhizal Fungi spore according to claim 1, which is characterized in that described to go out
Clean river sand that soil, the partial size of bacterium are 2mm, perlite, vermiculite ratio be 2:3:3:3.
4. a kind of method of fructus lycii Arbuscular Mycorrhizal Fungi spore according to claim 1, which is characterized in that the battalion
Nutrient solution is Gram nutrient solution suddenly, and the concentration of the nutrient solution of Gram suddenly is 1%.
5. a kind of method of fructus lycii Arbuscular Mycorrhizal Fungi spore according to claim 1, which is characterized in that described to post
The seed of main plant includes corn seed, alfalfa seed and clover seed, and two kinds of hosts therein are chosen in sowing in the S4
The seed of plant is sowed.
6. a kind of method of fructus lycii Arbuscular Mycorrhizal Fungi spore according to claim 1, which is characterized in that the clump
The growth temperature of mycorrhizal fungi is room temperature.
7. a kind of method of fructus lycii Arbuscular Mycorrhizal Fungi spore according to claim 1, which is characterized in that the S5
In to host plant irrigate standard be mild stress.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104371932A (en) * | 2014-10-21 | 2015-02-25 | 宁夏农林科学院 | Method for producing AM fungal inoculant |
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- 2019-04-10 CN CN201910285552.4A patent/CN110036812A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104371932A (en) * | 2014-10-21 | 2015-02-25 | 宁夏农林科学院 | Method for producing AM fungal inoculant |
Non-Patent Citations (1)
| Title |
|---|
| 王亚军等: "丛枝菌根真菌菌剂扩繁及菌根化枸杞育苗技术研究", 《北方园艺》 * |
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