CN110035825B - 用于处理微滴的微流体方法 - Google Patents

用于处理微滴的微流体方法 Download PDF

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Publication number
CN110035825B
CN110035825B CN201780074354.2A CN201780074354A CN110035825B CN 110035825 B CN110035825 B CN 110035825B CN 201780074354 A CN201780074354 A CN 201780074354A CN 110035825 B CN110035825 B CN 110035825B
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China
Prior art keywords
droplet
droplets
trapping
capture
trap
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CN201780074354.2A
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English (en)
Chinese (zh)
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CN110035825A (zh
Inventor
查尔斯·巴鲁德
拉斐尔·托马西
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Centre National de la Recherche Scientifique CNRS
Ecole Polytechnique
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Centre National de la Recherche Scientifique CNRS
Ecole Polytechnique
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/302—Micromixers the materials to be mixed flowing in the form of droplets
    • B01F33/3021—Micromixers the materials to be mixed flowing in the form of droplets the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/3035—Micromixers using surface tension to mix, move or hold the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0688—Valves, specific forms thereof surface tension valves, capillary stop, capillary break

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Fluid Mechanics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
  • Extraction Or Liquid Replacement (AREA)
CN201780074354.2A 2016-09-30 2017-09-29 用于处理微滴的微流体方法 Active CN110035825B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1659418 2016-09-30
FR1659418A FR3056927B1 (fr) 2016-09-30 2016-09-30 Procede microfluidique de manipulation de microgouttes
PCT/EP2017/074859 WO2018060471A1 (fr) 2016-09-30 2017-09-29 Procede microfluidique de manipulation de microgouttes

Publications (2)

Publication Number Publication Date
CN110035825A CN110035825A (zh) 2019-07-19
CN110035825B true CN110035825B (zh) 2021-10-26

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Application Number Title Priority Date Filing Date
CN201780074354.2A Active CN110035825B (zh) 2016-09-30 2017-09-29 用于处理微滴的微流体方法

Country Status (6)

Country Link
US (1) US11179722B2 (enExample)
EP (1) EP3519092A1 (enExample)
JP (2) JP7150707B2 (enExample)
CN (1) CN110035825B (enExample)
FR (1) FR3056927B1 (enExample)
WO (1) WO2018060471A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3566772A1 (en) * 2018-05-11 2019-11-13 Agilent Technologies, Inc. (A Delaware Corporation) Immobilizing fluidic sample for inhibiting spatial broadening
CN113330306B (zh) 2019-02-11 2024-09-27 安捷伦科技有限公司 处理前以平面方式预成型流体样品
FR3098128B1 (fr) 2019-07-05 2023-11-17 Commissariat Energie Atomique Dispositif microfluidique comportant une microgoutte présentant une matrice sol-gel.
EP4219685A1 (en) * 2022-01-31 2023-08-02 Institut Pasteur In vitro generation of organized 3d cell structures including head-trunk embryo-like structures, using epigenetic remodeling factors - microfluidic platform suitable for their generation
WO2023002057A2 (en) * 2021-07-22 2023-01-26 Institut Pasteur In vitro generation of organized 3d cell structures including head-trunk embryo-like structures, using epigenetic remodeling factors - microfluidic platform suitable for their generation
DE102022202862A1 (de) * 2022-03-24 2023-09-28 Robert Bosch Gesellschaft mit beschränkter Haftung Mikrofluidisches Aufnahmeelement, mikrofluidische Vorrichtung mit Aufnahmeelement, Verfahren zum Herstellen eines mikrofluidischen Aufnahmeelements und Verfahren zum Verwenden eines mikrofluidischen Aufnahmeelements
DE102022209421A1 (de) 2022-09-09 2024-03-14 Robert Bosch Gesellschaft mit beschränkter Haftung Array für eine mikrofluidische Vorrichtung

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9476811B2 (en) * 2010-10-01 2016-10-25 The Governing Council Of The University Of Toronto Digital microfluidic devices and methods incorporating a solid phase

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10326607A1 (de) * 2003-06-13 2005-01-05 Steag Microparts Gmbh Vorrichtung zum Handhaben von Flüssigkeiten
US8877512B2 (en) * 2009-01-23 2014-11-04 Advanced Liquid Logic, Inc. Bubble formation techniques using physical or chemical features to retain a gas bubble within a droplet actuator
JP2010273655A (ja) 2009-05-29 2010-12-09 Canon Inc 細胞保持方法、細胞試験方法及び細胞処理装置
FR2950544B1 (fr) 2009-09-29 2011-12-09 Ecole Polytech Circuit microfluidique
US9957472B2 (en) * 2011-09-22 2018-05-01 Georgia Tech Research Corporation Deterministic high-density single-cell trap array
FR2996544B1 (fr) * 2012-10-08 2015-03-13 Ecole Polytech Circuit microfluidique permettant la mise en contact de gouttes de plusieurs fluides, et procede microfluidique correspondant.
JP6078848B2 (ja) 2012-11-20 2017-02-15 公益財団法人神奈川科学技術アカデミー 脂質二重膜の形成方法及びそのための器具
US10710077B2 (en) * 2014-10-17 2020-07-14 Ecole Polytechnique Method for handling microdrops which include samples

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9476811B2 (en) * 2010-10-01 2016-10-25 The Governing Council Of The University Of Toronto Digital microfluidic devices and methods incorporating a solid phase

Also Published As

Publication number Publication date
FR3056927A1 (fr) 2018-04-06
JP7150707B2 (ja) 2022-10-11
JP7438280B2 (ja) 2024-02-26
WO2018060471A1 (fr) 2018-04-05
CN110035825A (zh) 2019-07-19
US11179722B2 (en) 2021-11-23
US20200038867A1 (en) 2020-02-06
JP2022136156A (ja) 2022-09-15
JP2019532805A (ja) 2019-11-14
EP3519092A1 (fr) 2019-08-07
FR3056927B1 (fr) 2021-07-09

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