FR3056927B1 - Procede microfluidique de manipulation de microgouttes - Google Patents

Procede microfluidique de manipulation de microgouttes Download PDF

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Publication number
FR3056927B1
FR3056927B1 FR1659418A FR1659418A FR3056927B1 FR 3056927 B1 FR3056927 B1 FR 3056927B1 FR 1659418 A FR1659418 A FR 1659418A FR 1659418 A FR1659418 A FR 1659418A FR 3056927 B1 FR3056927 B1 FR 3056927B1
Authority
FR
France
Prior art keywords
trapping
microdrop
drops
zone
microfluidic process
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1659418A
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English (en)
Other versions
FR3056927A1 (fr
Inventor
Charles Baroud
Raphael Tomasi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Ecole Polytechnique
Original Assignee
Centre National de la Recherche Scientifique CNRS
Ecole Polytechnique
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FR1659418A priority Critical patent/FR3056927B1/fr
Application filed by Centre National de la Recherche Scientifique CNRS, Ecole Polytechnique filed Critical Centre National de la Recherche Scientifique CNRS
Priority to JP2019517429A priority patent/JP7150707B2/ja
Priority to CN201780074354.2A priority patent/CN110035825B/zh
Priority to PCT/EP2017/074859 priority patent/WO2018060471A1/fr
Priority to US16/338,681 priority patent/US11179722B2/en
Priority to EP17777052.6A priority patent/EP3519092A1/fr
Publication of FR3056927A1 publication Critical patent/FR3056927A1/fr
Application granted granted Critical
Publication of FR3056927B1 publication Critical patent/FR3056927B1/fr
Priority to JP2022117270A priority patent/JP7438280B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/302—Micromixers the materials to be mixed flowing in the form of droplets
    • B01F33/3021—Micromixers the materials to be mixed flowing in the form of droplets the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/3035—Micromixers using surface tension to mix, move or hold the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0688—Valves, specific forms thereof surface tension valves, capillary stop, capillary break

Abstract

Procédé de manipulation d'au moins une première microgoutte (20) et d'au moins une deuxième microgoutte (25) dans un système microfluidique comportant un piège capillaire présentant une première zone de piégeage (15) et une deuxième zone de piégeage (18), le procédé comportant les étapes consistant à : (i) piéger la première microgoutte (20) dans la première zone de piégeage (15), et (ii) piéger la deuxième microgoutte (25) dans la deuxième zone de piégeage (18), la première et la deuxième zone de piégeage (15) étant agencées pour que la première et la deuxième microgoutte (20 ; 25) soient en contact l'une avec l'autre, la première et la deuxième zone de piégeage (15 ; 18) étant conformées pour que les forces de piégeage ramenées à l'une desdites microgouttes (20) soient différentes.
FR1659418A 2016-09-30 2016-09-30 Procede microfluidique de manipulation de microgouttes Active FR3056927B1 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
FR1659418A FR3056927B1 (fr) 2016-09-30 2016-09-30 Procede microfluidique de manipulation de microgouttes
CN201780074354.2A CN110035825B (zh) 2016-09-30 2017-09-29 用于处理微滴的微流体方法
PCT/EP2017/074859 WO2018060471A1 (fr) 2016-09-30 2017-09-29 Procede microfluidique de manipulation de microgouttes
US16/338,681 US11179722B2 (en) 2016-09-30 2017-09-29 Microfluidic method for handling microdrops
JP2019517429A JP7150707B2 (ja) 2016-09-30 2017-09-29 マイクロドロップを処理するためのマイクロ流体方法
EP17777052.6A EP3519092A1 (fr) 2016-09-30 2017-09-29 Procede microfluidique de manipulation de microgouttes
JP2022117270A JP7438280B2 (ja) 2016-09-30 2022-07-22 マイクロドロップを処理するためのマイクロ流体方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1659418A FR3056927B1 (fr) 2016-09-30 2016-09-30 Procede microfluidique de manipulation de microgouttes
FR1659418 2016-09-30

Publications (2)

Publication Number Publication Date
FR3056927A1 FR3056927A1 (fr) 2018-04-06
FR3056927B1 true FR3056927B1 (fr) 2021-07-09

Family

ID=58009902

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1659418A Active FR3056927B1 (fr) 2016-09-30 2016-09-30 Procede microfluidique de manipulation de microgouttes

Country Status (6)

Country Link
US (1) US11179722B2 (fr)
EP (1) EP3519092A1 (fr)
JP (2) JP7150707B2 (fr)
CN (1) CN110035825B (fr)
FR (1) FR3056927B1 (fr)
WO (1) WO2018060471A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3566772A1 (fr) * 2018-05-11 2019-11-13 Agilent Technologies, Inc. (A Delaware Corporation) Immobilisation d'échantillon fluidique pour inhiber un élargissement spatial
FR3098128B1 (fr) 2019-07-05 2023-11-17 Commissariat Energie Atomique Dispositif microfluidique comportant une microgoutte présentant une matrice sol-gel.
EP4219685A1 (fr) * 2022-01-31 2023-08-02 Institut Pasteur Production in vitro de structures cellulaires 3d organisées comprenant des structures embryonnaires tête-tronc, utilisant des facteurs de remodelage épigénétique - plateforme microfluidique appropriée pour leur production
WO2023002057A2 (fr) * 2021-07-22 2023-01-26 Institut Pasteur Génération in vitro de structures cellulaires 3d organisées comprenant des structures de type embryon tête-tronc, à l'aide de facteurs de remodelage épigénétiques - plateforme microfluidique convenant à leur génération
DE102022202862A1 (de) * 2022-03-24 2023-09-28 Robert Bosch Gesellschaft mit beschränkter Haftung Mikrofluidisches Aufnahmeelement, mikrofluidische Vorrichtung mit Aufnahmeelement, Verfahren zum Herstellen eines mikrofluidischen Aufnahmeelements und Verfahren zum Verwenden eines mikrofluidischen Aufnahmeelements
DE102022209421A1 (de) 2022-09-09 2024-03-14 Robert Bosch Gesellschaft mit beschränkter Haftung Array für eine mikrofluidische Vorrichtung

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10326607A1 (de) 2003-06-13 2005-01-05 Steag Microparts Gmbh Vorrichtung zum Handhaben von Flüssigkeiten
US8877512B2 (en) * 2009-01-23 2014-11-04 Advanced Liquid Logic, Inc. Bubble formation techniques using physical or chemical features to retain a gas bubble within a droplet actuator
JP2010273655A (ja) 2009-05-29 2010-12-09 Canon Inc 細胞保持方法、細胞試験方法及び細胞処理装置
FR2950544B1 (fr) 2009-09-29 2011-12-09 Ecole Polytech Circuit microfluidique
WO2012040861A1 (fr) * 2010-10-01 2012-04-05 The Governing Council Of The University Of Toronto Dispositifs microfluidiques numériques et procédés d'incorporation d'une phase solide
US9957472B2 (en) 2011-09-22 2018-05-01 Georgia Tech Research Corporation Deterministic high-density single-cell trap array
FR2996544B1 (fr) * 2012-10-08 2015-03-13 Ecole Polytech Circuit microfluidique permettant la mise en contact de gouttes de plusieurs fluides, et procede microfluidique correspondant.
JP6078848B2 (ja) 2012-11-20 2017-02-15 公益財団法人神奈川科学技術アカデミー 脂質二重膜の形成方法及びそのための器具
WO2016059302A1 (fr) * 2014-10-17 2016-04-21 Ecole Polytechnique Procédé de manipulation de microgouttes incluant des échantillons

Also Published As

Publication number Publication date
JP2019532805A (ja) 2019-11-14
JP7438280B2 (ja) 2024-02-26
FR3056927A1 (fr) 2018-04-06
US11179722B2 (en) 2021-11-23
JP2022136156A (ja) 2022-09-15
CN110035825A (zh) 2019-07-19
JP7150707B2 (ja) 2022-10-11
CN110035825B (zh) 2021-10-26
US20200038867A1 (en) 2020-02-06
EP3519092A1 (fr) 2019-08-07
WO2018060471A1 (fr) 2018-04-05

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