CN109997884A - A method of it is continuous using biostatic agent control rice field water - Google Patents

A method of it is continuous using biostatic agent control rice field water Download PDF

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Publication number
CN109997884A
CN109997884A CN201910282318.6A CN201910282318A CN109997884A CN 109997884 A CN109997884 A CN 109997884A CN 201910282318 A CN201910282318 A CN 201910282318A CN 109997884 A CN109997884 A CN 109997884A
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China
Prior art keywords
silk floss
rice field
biostatic agent
plant
water silk
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CN201910282318.6A
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CN109997884B (en
Inventor
范金霞
郭欣欣
王宏燕
郑国香
孙宏瑞
黄晓梅
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Northeast Agricultural University
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Northeast Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40Liliopsida [monocotyledons]
    • A01N65/44Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass

Abstract

The invention discloses a kind of methods using biostatic agent control rice field water silk floss; using stalk vinegar liquid and fresh plant fermentation liquid as water silk floss inhibitor; it can not only realize that the high value of stalk utilizes, while garbage classification and decrement can be pushed, moreover it is possible to promote greenization, the environmental protection of Rice Cropping;Water silk floss inhibitor of the invention has the characteristics that natural, environmental protection, production are simple;The many kinds of substance such as acids, alcohols, phenols, enzyme and microorganism are included in inhibitor, not only have the characteristics that inhibit the growth of water silk floss, while there is disease-resistant, expelling parasite, growth promotion, the multi-efficiency of purification of water quality.

Description

A method of it is continuous using biostatic agent control rice field water
Technical field
The present invention relates to agricultural technology fields, more particularly to a kind of to utilize the control of multifunctional natural biostatic agent The method of water silk floss in rice field processed.
Background technique
For many years, in the rice cultivation process, the dosage of chemical fertilizer is being continuously increased, and especially a large amount of of phosphate fertilizer make With so that the Water Eutrophication degree in rice field is on the rise, leading to the large area deposition of rice field water silk floss, affect the good of rice It is quite long and solid.Simultaneously because the use of chemical fertilizer and pesticide, the resistance of rice weakens, and the generation of pest and disease damage is increasingly Seriously, rice quality is caused to decline;These factors seriously constrain the Green Sustainable of China's rice industry.With multiple The use of the green agriculture input (pesticide, fertilizer) of effect is the inevitable choice of agriculture cycle development.
Stalk resource is the most abundant resource of reserves in the world, the comprehensive utilization of agricultural crop straw more and more attention has been paid to, Charcoal, the multi-products such as vinegar liquid and biological flue gas can be obtained in stalk after pyrolysis.Pile up like a mountain for house refuse, affects cities and towns Change construction and beautiful rural construction, is raw material using fresh plant waste, handling through microbial fermentation can be obtained fermentation liquid And fermentation solid.The use of environmentally protective input is to promote the key point of its sustainable development during Rice Cropping.
Therefore it provides a kind of method using multifunctional natural biostatic agent control rice field water silk floss is those skilled in the art The problem of member's urgent need to resolve.
Summary of the invention
In view of this, utilizing stalk vinegar the present invention provides a kind of method using biostatic agent control rice field water silk floss Liquid and fresh plant fermentation liquid can not only realize that the high value of stalk utilizes as water silk floss inhibitor, while can push rubbish point Class and decrement, moreover it is possible to promote greenization, the environmental protection of Rice Cropping.
To achieve the goals above, the present invention adopts the following technical scheme:
A method of it is continuous using biostatic agent control rice field water, the specific steps are as follows:
(1) crops stalk crushing is dried, is pyrolyzed after compression in pyrolysis oven, cooling collection pyrolysis liquids are quiet It only places 20-30 days, takes middle layer, obtain stalk vinegar liquid;
(2) plant waste and the buckwheat of crushing are mixed by the weight ratio of 8-9:1, obtains blends of plant materials;
(3) blends of plant materials and molasses and water obtained step (2) is mixed in the ratio of 3:1:10, is sufficiently stirred It mixes, after aerobic fermentation 30 days, carries out anaerobic fermentation being fully sealed 60 days, fermentation is completed to be separated by solid-liquid separation, and takes supernatant, obtains Plant fermented liquid;
(4) plant fermented liquid that stalk vinegar liquid and step (3) that step (1) obtains obtain is filled according to the ratio of 1:1-5 Divide mixing, the water that 5-8 times of volume is added is uniformly mixed, and obtains biostatic agent, is sealed cool place and is kept in dark place;
(5) biostatic agent that step (4) obtain is diluted to 100-200 times, Irrigated paddy field 2-3 times avoids rainy days It irrigates.
Further, step (1) specific steps for crushing drying are as follows: by crops stalk crushing to 1-2 centimetres, 100- 105 DEG C dry 4 hours.
Further, step (1) pyrolysis temperature is 400-500 DEG C, pyrolysis time 3-5h.
Further, step (2) described plant waste includes a variety of fresh samples such as dish leaf, pericarp.
Further, step (2) described plant waste is switched to 1-2 centimetres, and the buckwheat crushed 50 meshes.
Further, step (3) described separation of solid and liquid can be using modes such as solid-liquid separating machine, centrifuge separation or filterings.
Further, application of the stalk vinegar liquid in control rice field water silk floss.
Further, application of the plant fermented liquid in control rice field water silk floss
It can be seen via above technical scheme that compared with prior art, utilizing biology suppression the present disclosure provides a kind of The method of preparation control rice field water silk floss can not only be realized using stalk vinegar liquid and fresh plant fermentation liquid as water silk floss inhibitor The high value of stalk utilizes, while can push garbage classification and decrement, moreover it is possible to promote greenization, the environmental protection of Rice Cropping;This The water silk floss inhibitor of invention has the characteristics that natural, environmental protection, production are simple;Acids, alcohols, phenols, enzyme are included in inhibitor Many kinds of substance and microorganism are waited, not only has the characteristics that inhibit the growth of water silk floss, while there is disease-resistant, expelling parasite, growth promotion, water quality is net The multi-efficiency of change.Stalk vinegar liquid of the invention and plant fermented liquid are used alone, and can also achieve the effect that control water silk floss.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this The embodiment of invention for those of ordinary skill in the art without creative efforts, can also basis The attached drawing of offer obtains other attached drawings.
Fig. 1 attached drawing is that the different dilution stalk vinegar liquid processing of the present invention are lauched continuous metamorphosis;
Fig. 2 attached drawing is that the different dilution plant fermented liquid processing of the present invention are lauched continuous metamorphosis;
Fig. 3 attached drawing is the chlorophyll content during biostatic agent treated the water of different ratio of the present invention is continuous;
Fig. 4 attached drawing is the displaing micro picture of biostatic agent of the present invention water silk floss before and after the processing;
Wherein, A is that untreated water is continuous (blank control), and B is the displaing micro picture of water silk floss after biostatic agent processing.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other Embodiment shall fall within the protection scope of the present invention.
Embodiment 1
By crops stalk crushing to 1-2 centimetres, 100-105 DEG C is dried 4 hours, carries out heat in pyrolysis oven after compression Solution, pyrolysis temperature are 450 DEG C, pyrolysis time 4h;Cooling to collect pyrolysis liquids, static placement 25 days takes middle layer, obtains straw Stalk vinegar liquid.
The stalk vinegar liquid of acquisition is subjected to 100,300,500,700,900 times of dilutions respectively, processing water is continuous, with untreated work For control (CK), as a result as shown in Figure 1.
Fig. 1 the result shows that, stalk vinegar liquid difference dilution processing after water silk floss color turned yellow from green, water silk floss body is by hard Soften, black, the state of water silk floss body softness has been presented in 100 dilution Shi Shuimian, the growth of water silk floss is greatly inhibited.
Embodiment 2
The plant wastes such as dish leaf, pericarp are switched to 1-2 centimetres, buckwheat crushed 50 meshes, by plant waste and buckwheat Wheat is mixed by the weight ratio of 8:1, obtains blends of plant materials;Blends of plant materials and molasses and water are pressed to the ratio of 3:1:10 Example mixing, is sufficiently stirred, and after aerobic fermentation 30 days (being sufficiently stirred daily primary), carries out anaerobic fermentation being fully sealed 60 days, send out Ferment completion is separated by solid-liquid separation, and supernatant is taken, and obtains plant fermented liquid.
The plant fermented liquid of acquisition is carried out to 20,40,60,80,100 times of dilutions respectively, processing water is continuous, using it is untreated as It compares (CK), as a result as shown in Figure 2.
Fig. 2 the result shows that, obtained water silk floss color becomes yellow from green after the processing of 20-60 dilution plant fermentation liquid Color, water silk floss body are destroyed from hard to soft, the growth of water silk floss is affected also because of eucaryotic cell structure.
Embodiment 3
A method of it is continuous using biostatic agent control rice field water, the specific steps are as follows:
(1) by crops stalk crushing to 1-2 centimetres, 100-105 DEG C is dried 4 hours, is carried out after compression in pyrolysis oven Pyrolysis, pyrolysis temperature are 450 DEG C, pyrolysis time 4h;Cooling to collect pyrolysis liquids, static placement 25 days takes middle layer, obtains Stalk vinegar liquid;
(2) plant wastes such as dish leaf, pericarp are switched to 1-2 centimetres, buckwheat crushed 50 meshes, by plant waste and Buckwheat is mixed by the weight ratio of 8:1, obtains blends of plant materials;
(3) blends of plant materials and molasses and water obtained step (2) is mixed in the ratio of 3:1:10, is sufficiently stirred It mixes, after aerobic fermentation 30 days (being sufficiently stirred daily primary), carries out anaerobic fermentation being fully sealed 60 days, fermentation is completed to carry out solid-liquid Separation takes supernatant, obtains plant fermented liquid;
(4) by step (1) obtain stalk vinegar liquid and step (3) obtain plant fermented liquid press respectively 1:1,1:2,1:3, The ratio of 1:4,1:5 mix, and the water that 5 times of volumes are added is uniformly mixed, and obtain biostatic agent, and sealing cool place is kept in dark place;
(5) biostatic agent that step (4) obtain is diluted to 100 times, Irrigated paddy field 2 times, avoids irrigate rainy days.
Respectively at for 24 hours, 48h and 144h measure the chlorophyll content of processed water silk floss;Chlorophyll is the one of water silk floss growth A testing index, chlorophyll measuring method use acetone: ethyl alcohol (1:1) extracts colorimetric method, and measurement result is as shown in figure 3,144 is small When after chlorophyll content highest reduce 56%, hence it is evident that be lower than untreated (blank control group).
Water temperature, COD and pH are measured simultaneously, the results are shown in Table 1.
Table 1
Table 1 the result shows that, the biostatic agent of the embodiment of the present invention 3 make water temperature slightly improve, improve 0.4-0.6 DEG C, pH value is declined, and the attenuating of the pH value of the raising and water body of water temperature facilitates the growth of rice shoot, while in water body COD is also decreased obviously, and improves aquatic environment, and COD measurement is measured using the potassium dichromate method (GB/T11914) of national standard.
Stalk vinegar liquid and plant fermented liquid are mixed in the ratio of 1:3,100 times are diluted to, after handling water silk floss 144h, by it 40 times of amplification is observed under an optical microscope, as a result as shown in Figure 4;The result shows that the structure of processed water silk floss body is watt Solution, it is suppressed that it grows.
Embodiment 4
A method of it is continuous using biostatic agent control rice field water, the specific steps are as follows:
(2) by crops stalk crushing to 1-2 centimetres, 100-105 DEG C is dried 4 hours, is carried out after compression in pyrolysis oven Pyrolysis, pyrolysis temperature are 500 DEG C, pyrolysis time 3h;Cooling to collect pyrolysis liquids, static placement 30 days takes middle layer, obtains Stalk vinegar liquid;
(2) plant wastes such as dish leaf, pericarp are switched to 1-2 centimetres, buckwheat crushed 50 meshes, by plant waste and Buckwheat is mixed by the weight ratio of 9:1, obtains blends of plant materials;
(3) blends of plant materials and molasses and water obtained step (2) is mixed in the ratio of 3:1:10, is sufficiently stirred It mixes, after aerobic fermentation 30 days (being sufficiently stirred daily primary), carries out anaerobic fermentation being fully sealed 60 days, fermentation is completed to carry out solid-liquid Separation takes supernatant, obtains plant fermented liquid;
(4) by step (1) obtain stalk vinegar liquid and step (3) obtain plant fermented liquid press respectively 1:1,1:2,1:3, The ratio of 1:4,1:5 mix, and the water that 8 times of volumes are added is uniformly mixed, and obtain biostatic agent, and sealing cool place is kept in dark place;
(5) biostatic agent that step (4) obtain is diluted to 200 times, Irrigated paddy field 3 times, avoids irrigate rainy days.
The biostatic agent being prepared using embodiment 4 handles water silk floss, after handling 144h, the chlorophyll of water silk floss Content is substantially reduced, and water temperature slightly improves, and pH value is declined, and COD is also decreased obviously.
The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, as defined herein General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase one The widest scope of cause.

Claims (7)

1. a kind of method using biostatic agent control rice field water silk floss, which is characterized in that specific step is as follows:
(1) crops stalk crushing is dried, is pyrolyzed after compression, it is cooling to collect pyrolysis liquids, static placement 20-30 It, takes middle layer, obtains stalk vinegar liquid;
(2) plant waste and the buckwheat of crushing are mixed by the weight ratio of 8-9:1, obtains blends of plant materials;
(3) blends of plant materials and molasses and water obtained step (2) is mixed in the ratio of 3:1:10, is sufficiently stirred, good It after aerobe fermentation 30 days, carries out anaerobic fermentation being fully sealed 60 days, fermentation is completed to be separated by solid-liquid separation, and takes supernatant, obtains plant Fermentation liquid;
(4) plant fermented liquid that the stalk vinegar liquid and step (3) obtained step (1) obtains is sufficiently mixed according to the ratio of 1:1-5 It closes, the water that 5-8 times of volume is added is uniformly mixed, and obtains biostatic agent, and sealing cool place is kept in dark place;
(5) biostatic agent that step (4) obtain is diluted to 100-200 times, Irrigated paddy field 2-3 times.
2. a kind of method using biostatic agent control rice field water silk floss according to claim 1, which is characterized in that step (1) specific steps for crushing drying are as follows: by crops stalk crushing to 1-2 centimetres, 100-105 DEG C is dried 4 hours.
3. a kind of method using biostatic agent control rice field water silk floss according to claim 1, which is characterized in that step (1) pyrolysis temperature is 400-500 DEG C, pyrolysis time 3-5h.
4. a kind of method using biostatic agent control rice field water silk floss according to claim 1, which is characterized in that step (2) plant waste includes dish leaf, pericarp.
5. a kind of method using biostatic agent control rice field water silk floss according to claim 1, which is characterized in that step (2) plant waste is fresh sample, is switched to 1-2 centimetres, the buckwheat crushed 50 meshes.
6. application of the stalk vinegar liquid in control rice field water silk floss.
7. application of the plant fermented liquid in control rice field water silk floss.
CN201910282318.6A 2019-04-09 2019-04-09 Method for controlling paddy field spirogyra by using biological inhibitor Active CN109997884B (en)

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