CN109982713A - 外周血循环肿瘤细胞检测体系及其应用 - Google Patents

外周血循环肿瘤细胞检测体系及其应用 Download PDF

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Publication number
CN109982713A
CN109982713A CN201780072901.3A CN201780072901A CN109982713A CN 109982713 A CN109982713 A CN 109982713A CN 201780072901 A CN201780072901 A CN 201780072901A CN 109982713 A CN109982713 A CN 109982713A
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tumour cell
cancer
peripheral circulation
detection architecture
circulation tumour
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江陆斌
尹世刚
景庆庆
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Institut Pasteur of Shanghai of CAS
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Institut Pasteur of Shanghai of CAS
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
    • C07K14/445—Plasmodium

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
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  • Tropical Medicine & Parasitology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

提供了外周血循环肿瘤细胞检测体系。具体地，提供了包含缓冲体系和V2C偶联磁珠的检测体系，此外，还提供了V2C蛋白变体；其中，优选的缓冲体系包含：甘油、4‑羟乙基哌嗪乙磺酸(HEPES)和磷酸盐缓冲液，所述缓冲体系的pH为7.0‑7.6，优选pH为7.2‑7.4；且所述的V2C偶联磁珠为V2C蛋白或其变体与磁珠偶联得到的复合物。还提供了包含所述缓冲体系的检测试剂盒和反应体系。所述缓冲体系以及V2C偶联磁珠联合应用于CTC检测，与现有的CTC检测手段相比，具有更为优异的表现。

Description

PCT国内申请，说明书已公开。

Claims (61)

  1. PCT国内申请，权利要求书已公开。
CN201780072901.3A 2016-12-14 2017-11-10 外周血循环肿瘤细胞检测体系及其应用 Pending CN109982713A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2016111542243 2016-12-14
CN201611154224 2016-12-14
PCT/CN2017/110403 WO2018107930A1 (zh) 2016-12-14 2017-11-10 外周血循环肿瘤细胞检测体系及其应用

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CN109982713A true CN109982713A (zh) 2019-07-05

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CN (1) CN109982713A (zh)
WO (1) WO2018107930A1 (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113789303A (zh) * 2021-09-24 2021-12-14 安徽贝铭生物科技有限公司 一种提高血液循环肿瘤细胞俘获的方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113125384A (zh) * 2021-03-03 2021-07-16 汕头大学医学院 探头，循环肿瘤细胞检测设备及制备方法
CN113373156A (zh) * 2021-06-30 2021-09-10 四川携光生物技术有限公司 自身免疫性脑炎相关的nmdar重组蛋白、其编码序列、制法和应用

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CN103630440A (zh) * 2013-11-28 2014-03-12 武汉大学 一种循环肿瘤细胞的富集方法
CN104136041A (zh) * 2012-02-09 2014-11-05 Var2制药有限公司 靶向硫酸软骨素聚糖
CN204086278U (zh) * 2014-07-31 2015-01-07 北京海思特临床检验所有限公司 用于检测外周血循环肿瘤细胞的试剂盒

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CN104568923A (zh) * 2014-12-01 2015-04-29 浙江省肿瘤医院 电化学发光检测外周血循环肿瘤细胞抗原的方法及试剂盒

Patent Citations (3)

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CN104136041A (zh) * 2012-02-09 2014-11-05 Var2制药有限公司 靶向硫酸软骨素聚糖
CN103630440A (zh) * 2013-11-28 2014-03-12 武汉大学 一种循环肿瘤细胞的富集方法
CN204086278U (zh) * 2014-07-31 2015-01-07 北京海思特临床检验所有限公司 用于检测外周血循环肿瘤细胞的试剂盒

Non-Patent Citations (3)

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Title
CLAUSEN,T.M 等: "AFN44727.1 VAR2CSA, partial [synthetic construct]", 《NCBI》 *
杜晶辉 等: "微流控芯片分选富集循环肿瘤细胞的研究进展", 《色谱》 *
王振丹 等: "循环肿瘤细胞检测方法研究现状", 《中华肿瘤防治杂志》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113789303A (zh) * 2021-09-24 2021-12-14 安徽贝铭生物科技有限公司 一种提高血液循环肿瘤细胞俘获的方法

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Application publication date: 20190705