CN109937254B - 核酸样品制备方法 - Google Patents

核酸样品制备方法 Download PDF

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CN109937254B
CN109937254B CN201780070128.7A CN201780070128A CN109937254B CN 109937254 B CN109937254 B CN 109937254B CN 201780070128 A CN201780070128 A CN 201780070128A CN 109937254 B CN109937254 B CN 109937254B
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nucleic acid
target
primer
nucleotides
sequence
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CN109937254A (zh
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乔舒阿·斯塔尔
贾森·迈尔斯
布拉迪·卡尔弗
布赖恩·库德洛
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Asherdex Co ltd
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base
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    • C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
    • C12Q2549/10—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals
    • C12Q2549/119—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals using nested primers

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  • Crystallography & Structural Chemistry (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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CN201780070128.7A 2016-09-15 2017-09-15 核酸样品制备方法 Active CN109937254B (zh)

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US201662395339P 2016-09-15 2016-09-15
US62/395,339 2016-09-15
PCT/US2017/051924 WO2018053362A1 (en) 2016-09-15 2017-09-15 Methods of nucleic acid sample preparation

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CN109937254A CN109937254A (zh) 2019-06-25
CN109937254B true CN109937254B (zh) 2023-05-30

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US (2) US10704082B2 (https=)
EP (2) EP3512947B1 (https=)
JP (1) JP6997772B2 (https=)
CN (1) CN109937254B (https=)
AU (1) AU2017328950B2 (https=)
CA (1) CA3037185A1 (https=)
ES (1) ES2903357T3 (https=)
WO (1) WO2018053362A1 (https=)

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CA3037190A1 (en) * 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation for analysis of cell-free dna
WO2018053362A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation
US20220282305A1 (en) * 2016-09-15 2022-09-08 Archerdx, Llc Methods of nucleic acid sample preparation
EP4198140B1 (en) 2016-11-02 2024-09-04 ArcherDX, LLC Methods of nucleic acid sample preparation for immune repertoire sequencing
US20220228139A1 (en) * 2019-05-21 2022-07-21 Academia Sinica Method for amplifying and detecting ribonucleic acid (rna) fragments
WO2021027706A1 (zh) * 2019-08-09 2021-02-18 深圳市真迈生物科技有限公司 捕获核酸分子的方法、核酸文库的制备方法及测序方法
CN114929895A (zh) * 2019-11-05 2022-08-19 阿尔缇玛基因组学公司 用于制备多核苷酸分子文库的方法
CN115038795B (zh) * 2019-12-04 2026-03-20 贝克顿·迪金森公司 捕获磁珠介导的单颗粒中核酸靶标的分子条形码编码及用于其的组合物
MX2023001400A (es) * 2020-09-11 2023-04-25 Illumina Cambridge Ltd Metodos para enriquecer una secuencia diana a partir de una genoteca de secuenciación usando adaptadores de horquilla.
WO2022146773A1 (en) * 2020-12-29 2022-07-07 Nuprobe Usa, Inc. Methods and compositions for sequencing and fusion detection using ligation tail adapters (lta)
AU2022278434A1 (en) 2021-05-19 2023-11-23 Seqstant Gmbh Method for parallel real-time sequence analysis
CN118028444A (zh) * 2022-09-16 2024-05-14 中国疾病预防控制中心传染病预防控制所 一种基因测序方法、装置、设备和介质
EP4589020A1 (en) * 2022-09-16 2025-07-23 National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China Gene sequencing method, apparatus and device, and medium
USD1069156S1 (en) 2023-04-10 2025-04-01 Becton, Dickinson And Company Dispensing device
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