CN109913483A - 一种蛋白药物的发酵生产工艺 - Google Patents

一种蛋白药物的发酵生产工艺 Download PDF

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Publication number
CN109913483A
CN109913483A CN201711320516.4A CN201711320516A CN109913483A CN 109913483 A CN109913483 A CN 109913483A CN 201711320516 A CN201711320516 A CN 201711320516A CN 109913483 A CN109913483 A CN 109913483A
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CN
China
Prior art keywords
sdss1
albumen
cell
amino acid
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201711320516.4A
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English (en)
Chinese (zh)
Inventor
张英豪
李小灵
孙荣勋
宛佳
高海源
付晶鹏
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Puyou Biomedical Co ltd
Original Assignee
Shanghai Qingliu Biological Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Shanghai Qingliu Biological Pharmaceutical Co Ltd filed Critical Shanghai Qingliu Biological Pharmaceutical Co Ltd
Priority to CN201711320516.4A priority Critical patent/CN109913483A/zh
Priority to PCT/CN2018/117551 priority patent/WO2019114534A1/fr
Publication of CN109913483A publication Critical patent/CN109913483A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201711320516.4A 2017-12-12 2017-12-12 一种蛋白药物的发酵生产工艺 Pending CN109913483A (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201711320516.4A CN109913483A (zh) 2017-12-12 2017-12-12 一种蛋白药物的发酵生产工艺
PCT/CN2018/117551 WO2019114534A1 (fr) 2017-12-12 2018-11-27 Procédé de production de fermentation pour médicaments protéiques

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201711320516.4A CN109913483A (zh) 2017-12-12 2017-12-12 一种蛋白药物的发酵生产工艺

Publications (1)

Publication Number Publication Date
CN109913483A true CN109913483A (zh) 2019-06-21

Family

ID=66819894

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201711320516.4A Pending CN109913483A (zh) 2017-12-12 2017-12-12 一种蛋白药物的发酵生产工艺

Country Status (2)

Country Link
CN (1) CN109913483A (fr)
WO (1) WO2019114534A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112342263A (zh) * 2020-10-14 2021-02-09 江苏丰泽生物工程设备制造有限公司 一种实时预判发酵细菌生长趋势的方法

Citations (3)

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Publication number Priority date Publication date Assignee Title
JPH08217796A (ja) * 1995-02-14 1996-08-27 Sumitomo Electric Ind Ltd ヒトプロテアソームサブユニット
CN103215292A (zh) * 2012-01-18 2013-07-24 中国科学院生物物理研究所 人Pcid2蛋白的可溶性表达及抗人Pcid2蛋白的单克隆抗体2D7-F11和分泌该抗体的杂交瘤细胞系
CN107573412A (zh) * 2016-07-04 2018-01-12 上海清流生物医药科技有限公司 一种新型天然蛋白及其应用

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JPH0975082A (ja) * 1995-09-11 1997-03-25 Kyowa Hakko Kogyo Co Ltd ヒト２６ｓプロテアソーム構成成分蛋白質
AU2003231123A1 (en) * 2002-04-25 2003-11-10 Emory University Semisynthetic protein-based site-directed probes for identification and inhibition of active sites, and methods therefor
CN104628871B (zh) * 2015-02-09 2018-01-26 青岛明勤生物科技有限公司 一种重组法氏囊病蛋白工程疫苗的制备

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08217796A (ja) * 1995-02-14 1996-08-27 Sumitomo Electric Ind Ltd ヒトプロテアソームサブユニット
CN103215292A (zh) * 2012-01-18 2013-07-24 中国科学院生物物理研究所 人Pcid2蛋白的可溶性表达及抗人Pcid2蛋白的单克隆抗体2D7-F11和分泌该抗体的杂交瘤细胞系
CN107573412A (zh) * 2016-07-04 2018-01-12 上海清流生物医药科技有限公司 一种新型天然蛋白及其应用

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
/: "PREDICTED: Colobus angolensis palliatus split hand/foot malformation (ectrodactyly) type 1(SHFM1), transcript variant X1, mRNA，Accession No. XM_011943364.1", 《GENEBANK数据库》 *
/: "PREDICTED: Mandrillus leucophaeus split hand/foot malformation (ectrodactyly) type 1(SHFM1), transcript variant X1, mRNA，Accession No. XM_011974701.1", 《GENEBANK数据库》 *
/: "PREDICTED: Nomascus leucogenys split hand/foot malformation (ectrodactyly) type 1(SHFM1), transcript variant X1, mRNA，Accession No. XM_012510476.1", 《GENEBANK数据库》 *
/: "PREDICTED: Pan paniscus split hand/foot malformation (ectrodactyly) type 1(SHFM1), transcript variant X1, mRNA，Accession No. XM_008961980.1", 《GENEBANK数据库》 *
/: "PREDICTED: Pongo abelii split hand/foot malformation (ectrodactyly) type 1 (SHFM1), transcript variant X1, mRNA，Accession No. XM_009242993.1", 《GENEBANK数据库》 *
/: "PREDICTED: Rhinopithecus bieti split hand/foot malformation (ectrodactyly) type 1(SHFM1), transcript variant X1, mRNA，Accession No. XM_017849326.1", 《GENEBANK数据库》 *
CHENG-XIN GONG: "DSSylation, a novel guide for protein degradation?", 《PROTEIN AND CELL》 *
YINGHAO ZHANG等: "DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells", 《PROTEIN AND CELL》 *
李再新: "《制药分离工程实验》", 30 June 2016, 西南交通大学出版社 *
李思明: "《FGF21蛋白抗类风湿性关节炎研究》", 30 June 2017, 科学技术文献出版社 *
王星华: "栀子苷抗氧化保护神经细胞与DSSylation反应关系的研究", 《中国优秀硕士学位论文全文数据库》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112342263A (zh) * 2020-10-14 2021-02-09 江苏丰泽生物工程设备制造有限公司 一种实时预判发酵细菌生长趋势的方法
CN112342263B (zh) * 2020-10-14 2024-05-31 江苏丰泽生物工程设备制造有限公司 一种实时预判发酵细菌生长趋势的方法

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Address after: 201321 Room 420, Building 26, Shidai Medical Innovation Park, Lane 3399, Kangxin Road, Pudong New Area, Shanghai

Applicant after: Shanghai Puyou biomedical Co.,Ltd.

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Applicant before: SHANGHAI E-BLOT PHOTOELECTRIC TECHNOLOGY Co.,Ltd.

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Application publication date: 20190621

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