CN109891208A - 电子显微镜 - Google Patents

电子显微镜 Download PDF

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Publication number
CN109891208A
CN109891208A CN201780047251.7A CN201780047251A CN109891208A CN 109891208 A CN109891208 A CN 109891208A CN 201780047251 A CN201780047251 A CN 201780047251A CN 109891208 A CN109891208 A CN 109891208A
Authority
CN
China
Prior art keywords
sample
fluid
gas
electron microscope
carrier
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201780047251.7A
Other languages
English (en)
Chinese (zh)
Inventor
门德·雨果·拉默斯
拉斐尔·费尔南德斯-莱罗
约书亚·菲尔曼
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
British Research And Innovation Foundation
Original Assignee
British Research And Innovation Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by British Research And Innovation Foundation filed Critical British Research And Innovation Foundation
Publication of CN109891208A publication Critical patent/CN109891208A/zh
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G01N1/312—Apparatus therefor for samples mounted on planar substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Rigid containers without fluid transport within
    • B01L3/5085—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/50—Cryostats
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/523—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for multisample carriers, e.g. used for microtitration plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N30/12—Preparation by evaporation
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
    • H01J37/02—Details
    • H01J37/023—Means for mechanically adjusting components not otherwise provided for
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
    • H01J37/02—Details
    • H01J37/20—Means for supporting or positioning the object or the material; Means for adjusting diaphragms or lenses associated with the support
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1894—Cooling means; Cryo cooling
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J2237/00—Discharge tubes exposing object to beam, e.g. for analysis treatment, etching, imaging
    • H01J2237/002—Cooling arrangements

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
CN201780047251.7A 2016-07-29 2017-07-28 电子显微镜 Pending CN109891208A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1613173.2A GB201613173D0 (en) 2016-07-29 2016-07-29 Electron microscopy
GB1613173.2 2016-07-29
PCT/EP2017/069240 WO2018020036A1 (en) 2016-07-29 2017-07-28 Electron microscopy

Publications (1)

Publication Number Publication Date
CN109891208A true CN109891208A (zh) 2019-06-14

Family

ID=56936668

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201780047251.7A Pending CN109891208A (zh) 2016-07-29 2017-07-28 电子显微镜

Country Status (10)

Country Link
US (1) US11067486B2 (enExample)
EP (1) EP3491360B1 (enExample)
JP (1) JP6997757B2 (enExample)
CN (1) CN109891208A (enExample)
AU (1) AU2017302126B2 (enExample)
CA (1) CA3032234C (enExample)
DK (1) DK3491360T3 (enExample)
ES (1) ES3026360T3 (enExample)
GB (1) GB201613173D0 (enExample)
WO (1) WO2018020036A1 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3647763B1 (en) * 2018-10-29 2021-07-14 FEI Company A method of preparing a biological sample for study in an analysis device
CN111389478B (zh) * 2020-03-23 2023-07-25 西安医学院 一种组织切片的实验处理装置及其使用方法
GB2593491A (en) * 2020-03-24 2021-09-29 Res & Innovation Uk Electron microscopy support
EP4109069A1 (en) 2021-06-25 2022-12-28 FEI Company Blotting material with profiled region, method of manufacturing same, and uses thereof
NL2033291B1 (en) 2022-10-12 2024-04-26 Academisch Ziekenhuis Leiden Device and Method for Cryogenic Electron Microscopy Sample Preparation
EP4711738A1 (en) * 2024-09-17 2026-03-18 European Molecular Biology Laboratory Sample preparation method and device for preparing a vitrified sample layer on a sample holder grid for subsequent cryo electron microscopy

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070145268A1 (en) * 2005-12-09 2007-06-28 Lee, Bing-Huan Ultra-thin liquid control plate and combination of box-like member and the control plate
US20090126373A1 (en) * 2007-11-20 2009-05-21 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Ultra-rapid freezing device and method
GB2461708A (en) * 2008-07-08 2010-01-13 Silson Ltd Sample holder
US20100181495A1 (en) * 2009-01-22 2010-07-22 Leica Mikrosysteme Gmbh Device and method for preparing specimens
US20100302534A1 (en) * 2009-05-29 2010-12-02 Canon Kabushiki Kaisha Sample observation system and sample observation process
CN104517791A (zh) * 2013-09-30 2015-04-15 Fei公司 用于带电粒子显微术的冷冻样本的制备
CN104520966A (zh) * 2012-09-14 2015-04-15 株式会社日立高新技术 带电粒子束装置以及试样观察方法
CN105980827A (zh) * 2013-12-13 2016-09-28 文塔纳医疗系统公司 生物标本的自动化组织处理及关联技术的背景中的热管理
CN111122632A (zh) * 2018-10-29 2020-05-08 Fei 公司 制备用于在带电粒子装置中研究的生物样品的方法

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JPS59109840A (ja) 1982-12-16 1984-06-25 Hitachi Koki Co Ltd 走査形電子顕微鏡用生物試料の前処理方法
US7112790B1 (en) * 2003-08-13 2006-09-26 Cypress Semiconductor Corp. Method to prepare TEM samples
JP4891830B2 (ja) * 2007-04-18 2012-03-07 日本電子株式会社 電子顕微鏡用試料ホルダおよび観察方法
GB0724736D0 (en) * 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
JP2010054272A (ja) 2008-08-27 2010-03-11 National Institute Of Advanced Industrial Science & Technology スピン偏極走査電子顕微鏡
US9312095B2 (en) * 2010-03-24 2016-04-12 Brown University Method and system for automating sample preparation for microfluidic cryo TEM
GB201118282D0 (en) 2011-10-21 2011-12-07 Cancer Rec Tech Ltd Sample slide preparation method and device
JP6239246B2 (ja) * 2013-03-13 2017-11-29 株式会社日立ハイテクノロジーズ 荷電粒子線装置、試料観察方法、試料台、観察システム、および発光部材
GB201318463D0 (en) * 2013-08-13 2013-12-04 Medical Res Council Graphene Modification
EP3033762B1 (en) * 2013-08-13 2019-03-27 United Kingdom Research and Innovation Electron microscopy sample support comprising porous metal foil
GB2537579A (en) 2014-11-07 2016-10-26 Linkam Scient Instr Ltd Microscopic sample preparation
CN107533080A (zh) * 2015-04-20 2018-01-02 文塔纳医疗系统公司 用于组织学样品的试剂的喷墨沉积
EP3260839B1 (en) * 2016-06-22 2021-01-27 Universiteit Maastricht Method for preparing samples for imaging or diffraction experiments under cryogenic conditions

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070145268A1 (en) * 2005-12-09 2007-06-28 Lee, Bing-Huan Ultra-thin liquid control plate and combination of box-like member and the control plate
US20090126373A1 (en) * 2007-11-20 2009-05-21 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Ultra-rapid freezing device and method
GB2461708A (en) * 2008-07-08 2010-01-13 Silson Ltd Sample holder
US20100181495A1 (en) * 2009-01-22 2010-07-22 Leica Mikrosysteme Gmbh Device and method for preparing specimens
US20100302534A1 (en) * 2009-05-29 2010-12-02 Canon Kabushiki Kaisha Sample observation system and sample observation process
CN104520966A (zh) * 2012-09-14 2015-04-15 株式会社日立高新技术 带电粒子束装置以及试样观察方法
CN104517791A (zh) * 2013-09-30 2015-04-15 Fei公司 用于带电粒子显微术的冷冻样本的制备
CN105980827A (zh) * 2013-12-13 2016-09-28 文塔纳医疗系统公司 生物标本的自动化组织处理及关联技术的背景中的热管理
CN111122632A (zh) * 2018-10-29 2020-05-08 Fei 公司 制备用于在带电粒子装置中研究的生物样品的方法

Also Published As

Publication number Publication date
AU2017302126B2 (en) 2022-07-07
WO2018020036A1 (en) 2018-02-01
AU2017302126A1 (en) 2019-02-07
JP2019528435A (ja) 2019-10-10
EP3491360B1 (en) 2025-03-19
CA3032234A1 (en) 2018-02-01
US20190170618A1 (en) 2019-06-06
US11067486B2 (en) 2021-07-20
ES3026360T3 (en) 2025-06-11
DK3491360T3 (da) 2025-06-10
CA3032234C (en) 2024-02-13
GB201613173D0 (en) 2016-09-14
EP3491360A1 (en) 2019-06-05
JP6997757B2 (ja) 2022-02-04

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