CN109874909A - A kind of feed addictive and preparation process based on antibacterial peptide - Google Patents
A kind of feed addictive and preparation process based on antibacterial peptide Download PDFInfo
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Abstract
The invention discloses a kind of feed addictive and preparation process based on antibacterial peptide, the feed addictive include: 10-15 parts of antibacterial peptide, 6-12 parts of fruit of glossy privet extract, 5-10 parts of Chinese herbal medicine extract, 3-5 parts of Cordyceps Militaris solid fermentation culture, enzymatic hydrolysis pine needle meal 2-4 parts, 4-7 parts of soya-bean polypeptides, 0.5-1.5 parts of Se-enriched yeast powder, 0.4-0.8 parts of cellulase, 0.5-1 parts of compound amino acid, 0.3-0.5 parts of lysine chelated copper according to parts by weight.The present invention on the basis of antibacterial peptide by being equipped with the ingredients such as fruit of glossy privet extract, Chinese herbal medicine extract, Cordyceps Militaris solid fermentation culture, enzymatic hydrolysis pine needle meal, pass through each ingredient synergistic function, to improve feed addictive antibacterial ability of the invention, improve the immunity of livestock and poultry, enhance the constitution of livestock and poultry, and livestock and poultry can be improved to the utilization efficiency of feed, accelerate its speed of growth, to improve raise benefit.
Description
Technical field
The present invention relates to field of feed additive technology, and in particular to a kind of feed addictive and preparation based on antibacterial peptide
Technique.
Background technique
During modern livestock and poultry cultivation, in order to prevent and treat Animal diseases, excessive antibiotic product is often used to ensure
Animal health, but the use of excessive antibiotic often results in the quality decline of animal meat product and its product and antibiotic resistance increases
By force, human health is caused also to be seriously affected by antibiotic problem by the transmitting of food chain.
In order to reduce the residue problem of the antibiotic in livestock and poultry cultivation, productivity is improved, dirt of the poultry industry to environment is reduced
Dye, in recent years, developing feed addictive becomes a big hot spot.Wherein, the feed addictive based on antibacterial peptide increasingly by
To the attention of people.
Antibacterial peptide, which refers to be present in organism, can resist external microbe infringement, and the one kind for eliminating vivo mutations cell is small
Molecular polypeptide, it is the important component of innate immune system.Antibacterial peptide is usually made of 15-45 amino acid residue, greatly
Part is positively charged.A variety of biologies are as all contained antibacterial peptide in bacterium, insect, plant and vertebrate body.Since antibiotic is resistance to
Pharmacological property and residue problem become increasingly conspicuous, and as a kind of substitute of antibiotic, antibacterial peptide does not have the above-mentioned of antibiotic to lack because of it
It puts and is concerned.Antibiotic is applied for a long time in Production of Livestock and Poultry, but its long-time service also results in drug resistance and residual etc. sternly
Weight problem.It is similar to effects of antibiotics it is therefore desirable to find, and the not antibiotic substitute of the above problem.Antibacterial peptide is
A kind of antibacterial material being widely present in vivo is a kind of polypeptide of low molecular weight, equal to Gram-positive and negative bacterium
There is stronger lethal effect.
It is also in the industry cycle interior to be approved although the useful effect of antibacterial peptide has obtained universal confirmation, in recent years
Come, the application of antibacterial peptide only leans on single antibacterial peptide components still by biggish limitation, and antibacterial ability is limited, it is difficult to play and mention
High growth of animals or poultry speed, enhances the purpose of immunocompetence.
Based on this, it is necessary to a kind of feed addictive and preparation process based on antibacterial peptide is provided, to solve the prior art
The problem of.
Summary of the invention
In view of the above-mentioned analysis to the prior art, the present invention provides a kind of feed addictive based on antibacterial peptide, can pass through
Fruit of glossy privet extract, Chinese herbal medicine extract, Cordyceps Militaris solid fermentation culture, enzymatic hydrolysis pine needle meal etc. are equipped on the basis of antibacterial peptide
Ingredient, to improve feed addictive antibacterial ability of the invention, improves exempting from for livestock and poultry by each ingredient synergistic function
Epidemic disease power, enhances the constitution of livestock and poultry, and livestock and poultry can be improved to the utilization efficiency of feed, accelerates its speed of growth, to improve raising
Benefit.
In order to achieve the above object, the present invention is achieved through the following technical solutions:
A kind of feed addictive based on antibacterial peptide, the feed addictive include: according to parts by weight
10-15 parts of antibacterial peptide, 6-12 parts of fruit of glossy privet extract, 5-10 parts of Chinese herbal medicine extract, the training of cordyceps sinensis bacteria solid fermentation
Support object 3-5 parts, enzymatic hydrolysis pine needle meal 2-4 parts, 4-7 parts of soya-bean polypeptides, 0.5-1.5 parts of Se-enriched yeast powder, cellulase 0.4-0.8
Part, 0.5-1 parts of compound amino acid, 0.3-0.5 parts of lysine chelated copper；
Wherein, the Chinese herbal medicine extract be Shorthorned Epimedium P.E, Astragalus Root P.E and wrinkled giant hyssop leaf extract in mass ratio
3:2:1 is mixed.
Preferably, the feed addictive include: according to parts by weight 12.5 parts of antibacterial peptide, 9 parts of fruit of glossy privet extract, in
7.5 parts of herb extracts, 4 parts of Cordyceps Militaris solid fermentation culture, enzymatic hydrolysis 3 parts of pine needle meal, 5.5 parts of soya-bean polypeptides, Se-enriched yeast
1 part of powder, 0.6 part of cellulase, 0.7 part of compound amino acid, 0.4 part of lysine chelated copper.
Preferably, the Shorthorned Epimedium P.E the preparation method comprises the following steps: first by Herba Epimedii to be crushed after drinking water cleaning, drying
At coarse grain, 8 times of purified waters of Herba Epimedii are added, decoct 2 times, decoct 2 hours every time, merge decoction liquor, being concentrated into relative density is
1.15 medicinal extract, let cool, and 95% ethyl alcohol is added and carries out alcohol precipitation, stands 24 hours, extracts supernatant, is concentrated, and are concentrated into opposite
Density is 1.30, and medicinal extract is put into rail mounted film drier, is dried, and after dry, be ground into fine powder and mention to get Herba Epimedii
Take object；
The Astragalus Root P.E the preparation method comprises the following steps: Radix Astragali first crushed 40-50 mesh after, addition 8-10 times of Radix Astragali weigh
Amount 50% ethanol solution, under room temperature use 40-50kHz ultrasonic wave assisted extraction 2 times, each 30min, filter ethyl alcohol mentions
Liquid is taken, 5-8 times of weight hot water is added in the residue after extraction, 80 DEG C of decoction 1h, solution is mixed with ethanol extract after filtering,
100 DEG C of straight fire heating concentrated extracting solutions, conventional method are dry.60 meshes be crushed to get Astragalus Root P.E；
The wrinkled giant hyssop leaf extract the preparation method comprises the following steps: after Radix agastaches are crushed be added 5-8 times of pure water, with corase grinding grind,
And pueraria lobata is added, additional amount is ground after mixing through colloid mill with the 1-2% of wrinkled giant hyssop weight, adjusts colloid mill stator and rotor
Gap is 50-100 microns, and colloid mill flow control is 0.2-0.4 ton/hours, and the 0.05-0.2% of slurry weight is added later
Neutral proteinase digested, pH6.8-7.2,55-60 DEG C of temperature, enzymolysis time 45-55min, enzymolysis liquid enzyme deactivation, filtering,
It crushes after freeze concentration, freeze-drying to get wrinkled giant hyssop leaf extract.
Preferably, the Cordyceps Militaris solid fermentation culture the preparation method comprises the following steps:
(1) Cordyceps Militaris activation culture: the Cordyceps Militaris of preservation is transferred on culture medium, 23-27 DEG C of culture 40-45h, until bacterium
Filament length expires culture medium；
(2) one piece of above-mentioned cordyceps species picking access seed culture: is equipped with to 500 milliliters of shaking flasks of 100 milliliters of culture mediums
Middle carry out seed culture, condition of culture: 100-140 revs/min of rotary shaker, 23-27 DEG C of culture 60-70h；
(3) fermented and cultured: by shake-flask seed with the access of 5-10% inoculum concentration equipped with solid fermentation culture medium after sterilizing
In 500L solid-state fermentation tank, condition of culture: charge 45%, 22-26 DEG C of cultivation temperature, incubation time 20-30 days, every 2-5
It opens stirring 5min；
(4) fermented and cultured terminates, by dry materials to moisture content 5% hereinafter, crushing to solid fermentation material
Cross 40-60 mesh.
Preferably, in step (4) solid fermentation culture medium used carbon source selection from wheat bran, glucose, sucrose, starch,
Corncob after corn flour, crushing, nitrogen source selection are needed from groundnut meal, peptone, De-fatted soya protein Powder, beancake powder, yeast powder
Inorganic salts to be added have potassium dihydrogen phosphate, magnesium sulfate.
Preferably, the enzymatic hydrolysis pine needle meal the preparation method comprises the following steps: pine needle meal is placed in material-compound tank, pine needle meal weight is added
2-4 times of pure water, and medium temperature amylase is added, enzyme activity is 30u/g corn flour, is the protease of 30u/g corn flour, side with enzyme activity
Stirring side is warming up to 45-55 DEG C of heat preservation 20-25min, is then to slowly warm up to 45-50 DEG C of heat preservation 20-30min, is freeze-dried, i.e.,
Pine needle meal must be digested.
Preferably, the compound amino acid is grouped as by the group of following weight percentages: lysine 42%, methionine
44% and threonine 14%.
Preferably, the present invention also provides the preparation processes of the above-mentioned feed addictive based on antibacterial peptide, including following step
It is rapid:
(1) fruit of glossy privet extract, Chinese herbal medicine extract, Cordyceps Militaris solid fermentation culture and enzymatic hydrolysis pine needle meal are dried,
Drying temperature is 65 DEG C, is crushed after drying, crosses 50 meshes, obtains mixed powder；
(2) above-mentioned mixture is added in agitator tank, the water of mixed powder weight 25% is added, stirs evenly, access antibacterial
Peptide and soya-bean polypeptides, the ultrasonic disperse 10-15min at a temperature of 40-50 DEG C, obtain mixed serum；
(4) Se-enriched yeast powder, cellulase, compound amino acid, lysine chelated copper are added in Xiang Shangshu mixed serum, mixes
It closes uniformly, then puts into hardening and tempering furnace, it is quenched to be passed through 10% vapor that temperature is 85 DEG C, quality is each component gross mass
8min, then make material 95-105 DEG C at a temperature of enter pelletizer in pelletize；
(5) the feed addictive particle after granulation is dried to get the feed addictive based on antibacterial peptide.
Compared with prior art, the present invention have it is following the utility model has the advantages that
1, the present invention provides a kind of feed addictive based on antibacterial peptide, can be by being equipped with the fruit of glossy privet on the basis of antibacterial peptide
The ingredients such as extract, Chinese herbal medicine extract, Cordyceps Militaris solid fermentation culture, enzymatic hydrolysis pine needle meal, are cooperateed with by each ingredient and are increased
Effect effect, to improve feed addictive antibacterial ability of the invention, improves the immunity of livestock and poultry, enhances the constitution of livestock and poultry, and
Livestock and poultry can be improved to the utilization efficiency of feed, accelerate its speed of growth, to improve raise benefit；
2, the present invention is passed according to the mechanism of Chinese medicine of veterinary medicine with reference to Chinese medicine according to the growth characteristic and susceptible illness of livestock and poultry
System, has selected several drugs to be combined, and wherein fruit of glossy privet extract, which has, improves immunity of livestock, and fruit of glossy privet extract has
There are enriching yin and nourishing kidney, strengthening muscles and bones, accelerates the effect of the speed of growth of livestock and poultry；And the Chinese herbal medicine extract used is by by Herba Epimedii
Extract, Astragalus Root P.E and wrinkled giant hyssop leaf extract 3:2:1 in mass ratio mixing, combine Herba Epimedii, Radix Astragali and Radix agastaches three
The advantages of person, can effectively enhance the immunity function of livestock and poultry body, improve livestock and poultry resisting stress, anti-disease ability, improve the life of livestock and poultry
Performance is produced, while by the Chinese herbal medicine extract of arranging in pairs or groups in more bacterium peptides, can effectively improve feed addictive of the invention
Anti-microbial property, alternative traditional antibiotic medicine, and the Chinese herbal medicine extract have that noresidue, not generate drug resistance etc. excellent
Point；And poultry can be effectively improved containing a variety of active ingredients such as polysaccharide, cordycepic acid, cordycepins in Cordyceps Militaris solid fermentation culture
The immune function of fowl body promotes livestock and poultry metabolism, increases its food ration, to accelerate its speed of production；And the enzyme being added
Solution pine needle meal can be effectively improved the intestinal environment of livestock and poultry, promote the digestion and absorption efficiency of feed, boost metabolism, and enhance livestock and poultry
The immunity of body accelerates its speed of growth.The above drug is not simply individually had an effect, but is acted synergistically, to mention
The anti-microbial property of high feed addictive of the invention, and improve immunity, the somatotrophic purpose of livestock and poultry.
3, the Se-enriched yeast powder that the present invention adds is selenium-rich substance, can effectively enhance the immunocompetence of livestock and poultry, to improve
The disease resistance of livestock and poultry；And the absorption of the gastrointestinal tract of livestock and poultry can be improved in the cellulase being added, and improves the conversion ratio of feed；And
The compound amino acid of addition can effectively supplement amino acid required in livestock and poultry body.
Specific embodiment
Form by the following examples is described in further detail above content of the invention again, but should not be by this
The range for being interpreted as the above-mentioned theme of the present invention is only limitted to following embodiment, all technologies based on realization belonging to above content of the present invention
It all belongs to the scope of the present invention.
Embodiment 1
The feed addictive based on antibacterial peptide of the present embodiment, the feed addictive include: antibacterial according to parts by weight
10 parts of peptide, 6 parts of fruit of glossy privet extract, 5 parts of Chinese herbal medicine extract, 3 parts of Cordyceps Militaris solid fermentation culture, enzymatic hydrolysis 2 parts of pine needle meal,
4 parts of soya-bean polypeptides, 0.5 part of Se-enriched yeast powder, 0.4 part of cellulase, 0.5 part of compound amino acid, 0.3 part of lysine chelated copper.
Wherein, the Chinese herbal medicine extract be Shorthorned Epimedium P.E, Astragalus Root P.E and wrinkled giant hyssop leaf extract in mass ratio
3:2:1 is mixed.
Wherein, the Shorthorned Epimedium P.E the preparation method comprises the following steps: first by Herba Epimedii to be ground into after drinking water cleaning, drying
8 times of purified waters of Herba Epimedii are added in coarse grain, decoct 2 times, decoct 2 hours every time, merge decoction liquor, being concentrated into relative density is
1.15 medicinal extract, let cool, and 95% ethyl alcohol is added and carries out alcohol precipitation, stands 24 hours, extracts supernatant, is concentrated, and are concentrated into opposite
Density is 1.30, and medicinal extract is put into rail mounted film drier, is dried, and after dry, be ground into fine powder and mention to get Herba Epimedii
Take object.
The Astragalus Root P.E the preparation method comprises the following steps: Radix Astragali first crushed 40-50 mesh after, addition 8-10 times of Radix Astragali weigh
50% ethanol solution of amount is used 40kHz ultrasonic wave assisted extraction 2 times, each 30min under room temperature, filters to obtain ethyl alcohol extraction
Liquid adds 5 times of weight hot water in the residue after extraction, and 80 DEG C of decoction 1h, solution is mixed with ethanol extract after filtering, and 100 DEG C
Straight fire heating concentrated extracting solution, conventional method are dry.60 meshes be crushed to get Astragalus Root P.E.
The wrinkled giant hyssop leaf extract the preparation method comprises the following steps: after Radix agastaches are crushed be added 5 times of pure water, with corase grinding grind, and
Pueraria lobata is added, additional amount is ground after mixing through colloid mill with the 1% of wrinkled giant hyssop weight, adjusts the gap of colloid mill stator and rotor
It is 50-100 microns, colloid mill flow control is 0.2 ton/hour, and 0.05% neutral proteinase of slurry weight is added later
It is digested, pH6.8-7.2,55 DEG C of temperature, enzymolysis time 45min, enzymolysis liquid enzyme deactivation, filtering, after freeze concentration, freeze-drying
It crushes to get wrinkled giant hyssop leaf extract.
Wherein, the Cordyceps Militaris solid fermentation culture the preparation method comprises the following steps:
(1) Cordyceps Militaris activation culture: the Cordyceps Militaris of preservation is transferred on culture medium, 23 DEG C of culture 40h, until mycelia is covered with
Culture medium；
(2) one piece of above-mentioned cordyceps species picking access seed culture: is equipped with to 500 milliliters of shaking flasks of 100 milliliters of culture mediums
Middle carry out seed culture, condition of culture: 100 revs/min of rotary shaker, 23 DEG C of culture 60h；
(3) fermented and cultured: shake-flask seed is consolidated with the access of 5% inoculum concentration equipped with the 500L of solid fermentation culture medium after sterilizing
In body fermentor, condition of culture: charge 45%, incubation time 20 days, stirs 5min every unlatching in 2 days by 22 DEG C of cultivation temperature；
(4) fermented and cultured terminates, by dry materials to moisture content 5% hereinafter, crushing to solid fermentation material
Cross 40-60 mesh.
Wherein, in step (4) the carbon source selection of solid fermentation culture medium used from wheat bran, glucose, sucrose, starch, jade
Corncob after rice flour, crushing, nitrogen source select to need from groundnut meal, peptone, De-fatted soya protein Powder, beancake powder, yeast powder
The inorganic salts of addition have potassium dihydrogen phosphate, magnesium sulfate.
Wherein, the enzymatic hydrolysis pine needle meal the preparation method comprises the following steps: pine needle meal is placed in material-compound tank, pine needle meal weight 2 is added
Pure water again, and medium temperature amylase is added, enzyme activity is 30u/g corn flour, is the protease of 30u/g corn flour with enzyme activity, while stirring
It mixes side and is warming up to 45 DEG C of heat preservation 20min, be then to slowly warm up to 45 DEG C of heat preservation 20min, freeze-drying is to get enzymatic hydrolysis pine needle meal.
Wherein, the compound amino acid is grouped as by the group of following weight percentages: lysine 42%, methionine 44%
With threonine 14%.
Wherein, the present invention also provides the preparation processes of the above-mentioned feed addictive based on antibacterial peptide, comprising the following steps:
(1) fruit of glossy privet extract, Chinese herbal medicine extract, Cordyceps Militaris solid fermentation culture and enzymatic hydrolysis pine needle meal are dried,
Drying temperature is 65 DEG C, is crushed after drying, crosses 50 meshes, obtains mixed powder；
(2) above-mentioned mixture is added in agitator tank, the water of mixed powder weight 25% is added, stirs evenly, access antibacterial
Peptide and soya-bean polypeptides, ultrasonic disperse 10min, obtains mixed serum at a temperature of 40 DEG C；
(4) Se-enriched yeast powder, cellulase, compound amino acid, lysine chelated copper are added in Xiang Shangshu mixed serum, mixes
It closes uniformly, then puts into hardening and tempering furnace, it is quenched to be passed through 10% vapor that temperature is 85 DEG C, quality is each component gross mass
8min, then make material 95 DEG C at a temperature of enter pelletizer in pelletize；
(5) the feed addictive particle after granulation is dried to get the feed addictive based on antibacterial peptide.
Embodiment 2
The feed addictive based on antibacterial peptide of the present embodiment, the feed addictive include: antibacterial according to parts by weight
15 parts of peptide, 12 parts of fruit of glossy privet extract, 10 parts of Chinese herbal medicine extract, 5 parts of Cordyceps Militaris solid fermentation culture, enzymatic hydrolysis pine needle meal 4
Part, 7 parts of soya-bean polypeptides, 1.5 parts of Se-enriched yeast powder, 0.8 part of cellulase, 1 part of compound amino acid, 0.5 part of lysine chelated copper.
Wherein, the Chinese herbal medicine extract be Shorthorned Epimedium P.E, Astragalus Root P.E and wrinkled giant hyssop leaf extract in mass ratio
3:2:1 is mixed.
Wherein, the Shorthorned Epimedium P.E the preparation method comprises the following steps: first by Herba Epimedii to be ground into after drinking water cleaning, drying
8 times of purified waters of Herba Epimedii are added in coarse grain, decoct 2 times, decoct 2 hours every time, merge decoction liquor, being concentrated into relative density is
1.15 medicinal extract, let cool, and 95% ethyl alcohol is added and carries out alcohol precipitation, stands 24 hours, extracts supernatant, is concentrated, and are concentrated into opposite
Density is 1.30, and medicinal extract is put into rail mounted film drier, is dried, and after dry, be ground into fine powder and mention to get Herba Epimedii
Take object.
The Astragalus Root P.E the preparation method comprises the following steps: Radix Astragali first crushed 40-50 mesh after, add 8 times of weight of Radix Astragali
50% ethanol solution, under room temperature use 50kHz ultrasonic wave assisted extraction 2 times, each 30min filters to obtain ethanol extract,
5 times of weight hot water are added in residue after extraction, 80 DEG C of decoction 1h, solution is mixed with ethanol extract after filtering, 100 DEG C of straight fire
Concentrated extracting solution is heated, conventional method is dry.60 meshes be crushed to get Astragalus Root P.E.
The wrinkled giant hyssop leaf extract the preparation method comprises the following steps: after Radix agastaches are crushed be added 8 times of pure water, with corase grinding grind, and
Pueraria lobata is added, additional amount is ground after mixing through colloid mill with the 2% of wrinkled giant hyssop weight, adjusts the gap of colloid mill stator and rotor
Be 50-100 microns, colloid mill flow control be 0.4 ton/hour, later be added slurry weight 0.2% neutral proteinase into
Row enzymatic hydrolysis, pH6.8-7.2, temperature 60 C, enzymolysis time 55min, enzymolysis liquid enzyme deactivation, filtering, powder after freeze concentration, freeze-drying
It is broken to get wrinkled giant hyssop leaf extract.
Wherein, the Cordyceps Militaris solid fermentation culture the preparation method comprises the following steps:
(1) Cordyceps Militaris activation culture: the Cordyceps Militaris of preservation is transferred on culture medium, 27 DEG C of culture 45h, until mycelia is covered with
Culture medium；
(2) one piece of above-mentioned cordyceps species picking access seed culture: is equipped with to 500 milliliters of shaking flasks of 100 milliliters of culture mediums
Middle carry out seed culture, condition of culture: 140 revs/min of rotary shaker, 27 DEG C of culture 70h；
(3) fermented and cultured: the 500L by shake-flask seed with the access of 10% inoculum concentration equipped with solid fermentation culture medium after sterilizing
In solid-state fermentation tank, condition of culture: charge 45%, incubation time 30 days, was stirred every unlatching in 5 days by 26 DEG C of cultivation temperature
5min；
(4) fermented and cultured terminates, by dry materials to moisture content 5% hereinafter, crushing to solid fermentation material
Cross 40-60 mesh.
Wherein, in step (4) the carbon source selection of solid fermentation culture medium used from wheat bran, glucose, sucrose, starch, jade
Corncob after rice flour, crushing, nitrogen source select to need from groundnut meal, peptone, De-fatted soya protein Powder, beancake powder, yeast powder
The inorganic salts of addition have potassium dihydrogen phosphate, magnesium sulfate.
Wherein, the enzymatic hydrolysis pine needle meal the preparation method comprises the following steps: pine needle meal is placed in material-compound tank, pine needle meal weight 2- is added
4 times of pure water, and medium temperature amylase is added, enzyme activity is 30u/g corn flour, is the protease of 30u/g corn flour with enzyme activity, while stirring
It mixes side and is warming up to 55 DEG C of heat preservation 25min, be then to slowly warm up to 50 DEG C of heat preservation 30min, freeze-drying is to get enzymatic hydrolysis pine needle meal.
Wherein, the compound amino acid is grouped as by the group of following weight percentages: lysine 42%, methionine 44%
With threonine 14%.
Wherein, the present invention also provides the preparation processes of the above-mentioned feed addictive based on antibacterial peptide, comprising the following steps:
(1) fruit of glossy privet extract, Chinese herbal medicine extract, Cordyceps Militaris solid fermentation culture and enzymatic hydrolysis pine needle meal are dried,
Drying temperature is 65 DEG C, is crushed after drying, crosses 50 meshes, obtains mixed powder；
(2) above-mentioned mixture is added in agitator tank, the water of mixed powder weight 25% is added, stirs evenly, access antibacterial
Peptide and soya-bean polypeptides, ultrasonic disperse 15min, obtains mixed serum at a temperature of 50 DEG C；
(4) Se-enriched yeast powder, cellulase, compound amino acid, lysine chelated copper are added in Xiang Shangshu mixed serum, mixes
It closes uniformly, then puts into hardening and tempering furnace, it is quenched to be passed through 10% vapor that temperature is 85 DEG C, quality is each component gross mass
8min, then make material 105 DEG C at a temperature of enter pelletizer in pelletize；
(5) the feed addictive particle after granulation is dried to get the feed addictive based on antibacterial peptide.
Embodiment 3
The feed addictive based on antibacterial peptide of the present embodiment, the feed addictive include: antibacterial according to parts by weight
12.5 parts of peptide, 9 parts of fruit of glossy privet extract, 7.5 parts of Chinese herbal medicine extract, 4 parts of Cordyceps Militaris solid fermentation culture, enzymatic hydrolysis pine needle meal
3 parts, 5.5 parts of soya-bean polypeptides, 1 part of Se-enriched yeast powder, 0.6 part of cellulase, 0.7 part of compound amino acid, lysine chelated copper 0.4
Part.
Wherein, the Chinese herbal medicine extract be Shorthorned Epimedium P.E, Astragalus Root P.E and wrinkled giant hyssop leaf extract in mass ratio
3:2:1 is mixed.
Wherein, the Shorthorned Epimedium P.E the preparation method comprises the following steps: first by Herba Epimedii to be ground into after drinking water cleaning, drying
8 times of purified waters of Herba Epimedii are added in coarse grain, decoct 2 times, decoct 2 hours every time, merge decoction liquor, being concentrated into relative density is
1.15 medicinal extract, let cool, and 95% ethyl alcohol is added and carries out alcohol precipitation, stands 24 hours, extracts supernatant, is concentrated, and are concentrated into opposite
Density is 1.30, and medicinal extract is put into rail mounted film drier, is dried, and after dry, be ground into fine powder and mention to get Herba Epimedii
Take object.
The Astragalus Root P.E the preparation method comprises the following steps: Radix Astragali first crushed 40-50 mesh after, add 9 times of weight of Radix Astragali
50% ethanol solution, under room temperature use 45kHz ultrasonic wave assisted extraction 2 times, each 30min filters to obtain ethanol extract,
6.5 times of weight hot water are added in residue after extraction, 80 DEG C of decoction 1h, solution is mixed with ethanol extract after filtering, and 100 DEG C straight
Fire heating concentrated extracting solution, conventional method are dry.60 meshes be crushed to get Astragalus Root P.E.
The wrinkled giant hyssop leaf extract the preparation method comprises the following steps: after Radix agastaches are crushed be added 6.5 times of pure water, with corase grinding grind,
And pueraria lobata is added, additional amount is ground after mixing through colloid mill with the 1.5% of wrinkled giant hyssop weight, adjusts colloid mill stator and rotor
Gap is 50-100 microns, and colloid mill flow control is 0.3 ton/hour, and 0.12% neutral egg of slurry weight is added later
White enzyme is digested, pH6.8-7.2, and 58 DEG C of temperature, enzymolysis time 50min, enzymolysis liquid enzyme deactivation, filtering, freeze concentration, freezing are dry
It crushes after dry to get wrinkled giant hyssop leaf extract.
Wherein, the Cordyceps Militaris solid fermentation culture the preparation method comprises the following steps:
(1) Cordyceps Militaris activation culture: the Cordyceps Militaris of preservation is transferred on culture medium, 25 DEG C of culture 43h, until mycelia is covered with
Culture medium；
(2) one piece of above-mentioned cordyceps species picking access seed culture: is equipped with to 500 milliliters of shaking flasks of 100 milliliters of culture mediums
Middle carry out seed culture, condition of culture: 120 revs/min of rotary shaker, 25 DEG C of culture 65h；
(3) fermented and cultured: the 500L by shake-flask seed with the access of 7.5% inoculum concentration equipped with solid fermentation culture medium after sterilizing
In solid-state fermentation tank, condition of culture: charge 45%, incubation time 25 days, was stirred every unlatching in 4 days by 24 DEG C of cultivation temperature
5min；
(4) fermented and cultured terminates, by dry materials to moisture content 5% hereinafter, crushing to solid fermentation material
Cross 40-60 mesh.
Wherein, in step (4) the carbon source selection of solid fermentation culture medium used from wheat bran, glucose, sucrose, starch, jade
Corncob after rice flour, crushing, nitrogen source select to need from groundnut meal, peptone, De-fatted soya protein Powder, beancake powder, yeast powder
The inorganic salts of addition have potassium dihydrogen phosphate, magnesium sulfate.
Wherein, the enzymatic hydrolysis pine needle meal the preparation method comprises the following steps: pine needle meal is placed in material-compound tank, pine needle meal weight 3 is added
Pure water again, and medium temperature amylase is added, enzyme activity is 30u/g corn flour, is the protease of 30u/g corn flour with enzyme activity, while stirring
It mixes side and is warming up to 50 DEG C of heat preservation 22min, be then to slowly warm up to 48 DEG C of heat preservation 25min, freeze-drying is to get enzymatic hydrolysis pine needle meal.
Wherein, the compound amino acid is grouped as by the group of following weight percentages: lysine 42%, methionine 44%
With threonine 14%.
Wherein, the present invention also provides the preparation processes of the above-mentioned feed addictive based on antibacterial peptide, comprising the following steps:
(1) fruit of glossy privet extract, Chinese herbal medicine extract, Cordyceps Militaris solid fermentation culture and enzymatic hydrolysis pine needle meal are dried,
Drying temperature is 65 DEG C, is crushed after drying, crosses 50 meshes, obtains mixed powder；
(2) above-mentioned mixture is added in agitator tank, the water of mixed powder weight 25% is added, stirs evenly, access antibacterial
Peptide and soya-bean polypeptides, ultrasonic disperse 13min, obtains mixed serum at a temperature of 45 DEG C；
(4) Se-enriched yeast powder, cellulase, compound amino acid, lysine chelated copper are added in Xiang Shangshu mixed serum, mixes
It closes uniformly, then puts into hardening and tempering furnace, it is quenched to be passed through 10% vapor that temperature is 85 DEG C, quality is each component gross mass
8min, then make material 100 DEG C at a temperature of enter pelletizer in pelletize；
(5) the feed addictive particle after granulation is dried to get the feed addictive based on antibacterial peptide.
Embodiment 4
The feed addictive based on antibacterial peptide of the present embodiment, the feed addictive include: antibacterial according to parts by weight
11 parts of peptide, 7 parts of fruit of glossy privet extract, 6 parts of Chinese herbal medicine extract, 3.5 parts of Cordyceps Militaris solid fermentation culture, enzymatic hydrolysis pine needle meal
2.5 parts, 5 parts of soya-bean polypeptides, 0.8 part of Se-enriched yeast powder, 0.5 part of cellulase, 0.6 part of compound amino acid, lysine chelated copper
0.4 part.
Wherein, the Chinese herbal medicine extract be Shorthorned Epimedium P.E, Astragalus Root P.E and wrinkled giant hyssop leaf extract in mass ratio
3:2:1 is mixed.
Wherein, the Shorthorned Epimedium P.E the preparation method comprises the following steps: first by Herba Epimedii to be ground into after drinking water cleaning, drying
8 times of purified waters of Herba Epimedii are added in coarse grain, decoct 2 times, decoct 2 hours every time, merge decoction liquor, being concentrated into relative density is
1.15 medicinal extract, let cool, and 95% ethyl alcohol is added and carries out alcohol precipitation, stands 24 hours, extracts supernatant, is concentrated, and are concentrated into opposite
Density is 1.30, and medicinal extract is put into rail mounted film drier, is dried, and after dry, be ground into fine powder and mention to get Herba Epimedii
Take object.
The Astragalus Root P.E the preparation method comprises the following steps: Radix Astragali first crushed 40-50 mesh after, addition 8-10 times of Radix Astragali weigh
50% ethanol solution of amount is used 43kHz ultrasonic wave assisted extraction 2 times, each 30min under room temperature, filters to obtain ethyl alcohol extraction
Liquid adds 6 times of weight hot water in the residue after extraction, and 80 DEG C of decoction 1h, solution is mixed with ethanol extract after filtering, and 100 DEG C
Straight fire heating concentrated extracting solution, conventional method are dry.60 meshes be crushed to get Astragalus Root P.E.
The wrinkled giant hyssop leaf extract the preparation method comprises the following steps: after Radix agastaches are crushed be added 6 times of pure water, with corase grinding grind, and
Pueraria lobata is added, additional amount is ground after mixing through colloid mill with the 1.2% of wrinkled giant hyssop weight, is adjusted between colloid mill stator and rotor
Gap is 50-100 microns, and colloid mill flow control is 0.3 ton/hour, and 0.08% neutral protein of slurry weight is added later
Enzyme is digested, pH6.8-7.2, and 56 DEG C of temperature, enzymolysis time 48min, enzymolysis liquid enzyme deactivation, filtering, freeze concentration, freeze-drying
After crush to get wrinkled giant hyssop leaf extract.
Wherein, the Cordyceps Militaris solid fermentation culture the preparation method comprises the following steps:
(1) Cordyceps Militaris activation culture: the Cordyceps Militaris of preservation is transferred on culture medium, 24 DEG C of culture 42h, until mycelia is covered with
Culture medium；
(2) one piece of above-mentioned cordyceps species picking access seed culture: is equipped with to 500 milliliters of shaking flasks of 100 milliliters of culture mediums
Middle carry out seed culture, condition of culture: 110 revs/min of rotary shaker, 24 DEG C of culture 62h；
(3) fermented and cultured: shake-flask seed is consolidated with the access of 6% inoculum concentration equipped with the 500L of solid fermentation culture medium after sterilizing
In body fermentor, condition of culture: charge 45%, incubation time 22 days, stirs 5min every unlatching in 3 days by 23 DEG C of cultivation temperature；
(4) fermented and cultured terminates, by dry materials to moisture content 5% hereinafter, crushing to solid fermentation material
Cross 40-60 mesh.
Wherein, in step (4) the carbon source selection of solid fermentation culture medium used from wheat bran, glucose, sucrose, starch, jade
Corncob after rice flour, crushing, nitrogen source select to need from groundnut meal, peptone, De-fatted soya protein Powder, beancake powder, yeast powder
The inorganic salts of addition have potassium dihydrogen phosphate, magnesium sulfate.
Wherein, the enzymatic hydrolysis pine needle meal the preparation method comprises the following steps: pine needle meal is placed in material-compound tank, pine needle meal weight 2- is added
4 times of pure water, and medium temperature amylase is added, enzyme activity is 30u/g corn flour, is the protease of 30u/g corn flour with enzyme activity, while stirring
It mixes side and is warming up to 48 DEG C of heat preservation 22min, be then to slowly warm up to 46 DEG C of heat preservation 23min, freeze-drying is to get enzymatic hydrolysis pine needle meal.
Wherein, the compound amino acid is grouped as by the group of following weight percentages: lysine 42%, methionine 44%
With threonine 14%.
Wherein, the present invention also provides the preparation processes of the above-mentioned feed addictive based on antibacterial peptide, comprising the following steps:
(1) fruit of glossy privet extract, Chinese herbal medicine extract, Cordyceps Militaris solid fermentation culture and enzymatic hydrolysis pine needle meal are dried,
Drying temperature is 65 DEG C, is crushed after drying, crosses 50 meshes, obtains mixed powder；
(2) above-mentioned mixture is added in agitator tank, the water of mixed powder weight 25% is added, stirs evenly, access antibacterial
Peptide and soya-bean polypeptides, ultrasonic disperse 11min, obtains mixed serum at a temperature of 43 DEG C；
(4) Se-enriched yeast powder, cellulase, compound amino acid, lysine chelated copper are added in Xiang Shangshu mixed serum, mixes
It closes uniformly, then puts into hardening and tempering furnace, it is quenched to be passed through 10% vapor that temperature is 85 DEG C, quality is each component gross mass
8min, then make material 98 DEG C at a temperature of enter pelletizer in pelletize；
(5) the feed addictive particle after granulation is dried to get the feed addictive based on antibacterial peptide.
Embodiment 5
The feed addictive based on antibacterial peptide of the present embodiment, the feed addictive include: antibacterial according to parts by weight
14 parts of peptide, 11 parts of fruit of glossy privet extract, 9 parts of Chinese herbal medicine extract, 4.5 parts of Cordyceps Militaris solid fermentation culture, enzymatic hydrolysis pine needle meal
3.5 parts, 6 parts of soya-bean polypeptides, 1.2 parts of Se-enriched yeast powder, 0.7 part of cellulase, 0.9 part of compound amino acid, lysine chelated copper
0.4 part.
Wherein, the Chinese herbal medicine extract be Shorthorned Epimedium P.E, Astragalus Root P.E and wrinkled giant hyssop leaf extract in mass ratio
3:2:1 is mixed.
Wherein, the Shorthorned Epimedium P.E the preparation method comprises the following steps: first by Herba Epimedii to be ground into after drinking water cleaning, drying
8 times of purified waters of Herba Epimedii are added in coarse grain, decoct 2 times, decoct 2 hours every time, merge decoction liquor, being concentrated into relative density is
1.15 medicinal extract, let cool, and 95% ethyl alcohol is added and carries out alcohol precipitation, stands 24 hours, extracts supernatant, is concentrated, and are concentrated into opposite
Density is 1.30, and medicinal extract is put into rail mounted film drier, is dried, and after dry, be ground into fine powder and mention to get Herba Epimedii
Take object.
The Astragalus Root P.E the preparation method comprises the following steps: Radix Astragali first crushed 40-50 mesh after, addition 8-10 times of Radix Astragali weigh
50% ethanol solution of amount is used 48kHz ultrasonic wave assisted extraction 2 times, each 30min under room temperature, filters to obtain ethyl alcohol extraction
Liquid adds 7 times of weight hot water in the residue after extraction, and 80 DEG C of decoction 1h, solution is mixed with ethanol extract after filtering, and 100 DEG C
Straight fire heating concentrated extracting solution, conventional method are dry.60 meshes be crushed to get Astragalus Root P.E.
The wrinkled giant hyssop leaf extract the preparation method comprises the following steps: after Radix agastaches are crushed be added 5-8 times of pure water, with corase grinding grind,
And pueraria lobata is added, additional amount is ground after mixing through colloid mill with the 1.8% of wrinkled giant hyssop weight, adjusts colloid mill stator and rotor
Gap is 50-100 microns, and colloid mill flow control is 0.4 ton/hour, and 0.15% neutral egg of slurry weight is added later
White enzyme is digested, pH6.8-7.2, and 58 DEG C of temperature, enzymolysis time 52min, enzymolysis liquid enzyme deactivation, filtering, freeze concentration, freezing are dry
It crushes after dry to get wrinkled giant hyssop leaf extract.
Wherein, the Cordyceps Militaris solid fermentation culture the preparation method comprises the following steps:
(1) Cordyceps Militaris activation culture: the Cordyceps Militaris of preservation is transferred on culture medium, 26 DEG C of culture 44h, until mycelia is covered with
Culture medium；
(2) one piece of above-mentioned cordyceps species picking access seed culture: is equipped with to 500 milliliters of shaking flasks of 100 milliliters of culture mediums
Middle carry out seed culture, condition of culture: 130 revs/min of rotary shaker, 26 DEG C of culture 68h；
(3) fermented and cultured: shake-flask seed is consolidated with the access of 9% inoculum concentration equipped with the 500L of solid fermentation culture medium after sterilizing
In body fermentor, condition of culture: charge 45%, incubation time 28 days, stirs 5min every unlatching in 4 days by 25 DEG C of cultivation temperature；
(4) fermented and cultured terminates, by dry materials to moisture content 5% hereinafter, crushing to solid fermentation material
Cross 40-60 mesh.
Wherein, in step (4) the carbon source selection of solid fermentation culture medium used from wheat bran, glucose, sucrose, starch, jade
Corncob after rice flour, crushing, nitrogen source select to need from groundnut meal, peptone, De-fatted soya protein Powder, beancake powder, yeast powder
The inorganic salts of addition have potassium dihydrogen phosphate, magnesium sulfate.
Wherein, the enzymatic hydrolysis pine needle meal the preparation method comprises the following steps: pine needle meal is placed in material-compound tank, pine needle meal weight 2- is added
4 times of pure water, and medium temperature amylase is added, enzyme activity is 30u/g corn flour, is the protease of 30u/g corn flour with enzyme activity, while stirring
It mixes side and is warming up to 52 DEG C of heat preservation 24min, be then to slowly warm up to 48 DEG C of heat preservation 28min, freeze-drying is to get enzymatic hydrolysis pine needle meal.
Wherein, the compound amino acid is grouped as by the group of following weight percentages: lysine 42%, methionine 44%
With threonine 14%.
Wherein, the present invention also provides the preparation processes of the above-mentioned feed addictive based on antibacterial peptide, comprising the following steps:
(1) fruit of glossy privet extract, Chinese herbal medicine extract, Cordyceps Militaris solid fermentation culture and enzymatic hydrolysis pine needle meal are dried,
Drying temperature is 65 DEG C, is crushed after drying, crosses 50 meshes, obtains mixed powder；
(2) above-mentioned mixture is added in agitator tank, the water of mixed powder weight 25% is added, stirs evenly, access antibacterial
Peptide and soya-bean polypeptides, ultrasonic disperse 14min, obtains mixed serum at a temperature of 48 DEG C；
(4) Se-enriched yeast powder, cellulase, compound amino acid, lysine chelated copper are added in Xiang Shangshu mixed serum, mixes
It closes uniformly, then puts into hardening and tempering furnace, it is quenched to be passed through 10% vapor that temperature is 85 DEG C, quality is each component gross mass
8min, then make material 102 DEG C at a temperature of enter pelletizer in pelletize；
(5) the feed addictive particle after granulation is dried to get the feed addictive based on antibacterial peptide.
Comparative example 1
In addition to saving fruit of glossy privet extract, other components and content are consistent with embodiment 1.
Comparative example 2
In addition to saving Chinese herbal medicine extract, other components and content are consistent with embodiment 1.
Comparative example 3
In addition to saving Cordyceps Militaris solid fermentation culture, other components and content are consistent with embodiment 1.
Comparative example 4
In addition to it will digest pine needle meal and save, other components and content are consistent with embodiment 1.
Test example 1
Feed addictive made from 1-5 of the embodiment of the present invention and comparative example 1-4 is subjected to sterilization test, the side specifically tested
Method are as follows: the feed addictive for the room temperature extraction embodiment 1-5 and comparative example 1-4 that fetches water respectively, the ratio of feed addictive and water
For 1:10, room temperature is extracted 4 hours, and after then filtering to leaching liquor, filtrate is crossed to 0.22 micron of filter, obtains sterile extraction filter
Liquid takes sterile extraction filtrate to carry out sterilization circle examination to staphylococcus aureus, Escherichia coli, salmonella and Shigella respectively
It tests, the size of record sterilization circle, concrete outcome see the table below 1.
1 sterilization test result of table-sterilization circle size (mm)
As shown in Table 1, by the above bacteriostatic test can, the feed addictive of 1-5 of the embodiment of the present invention is for common livestock and poultry
Susceptible germ: staphylococcus aureus, Escherichia coli, salmonella and Shigella all have good killing effect, and
Better than comparative example 1-4, it follows that by the way that fruit of glossy privet extract, Chinese herbal medicine extract, Cordyceps Militaris are added in feed addictive
Solid fermentation culture and enzymatic hydrolysis pine needle meal, can increase the sterilizing ability of feed addictive, and phase between above-mentioned four kinds of ingredients
Interaction has the effect of significantly cooperateing with, enhancing for bactericidal activity, extent of killing bacteria significantly better than individually it is one of at
Point.
Test example 2
Test is divided into experimental group 1-5, control group 1-4 and is tested for totally 9 groups, and every group to select 1 monthly age, weight close, strong
The piglet of health in order 30, wherein blank control group is using experimental group using common commercially available pannage, experimental group 1-5
It is being mixed with common commercially available pannage according to the mass ratio of 1:6 based on the feed addictive of antibacterial peptide for embodiment 1-5 respectively
It is fed after uniformly；Control group 1-4 be respectively adopted comparative example 1-4 based on the feed addictive of antibacterial peptide with it is common commercially available
Pannage is fed after mixing according to the mass ratio of 1:6, and feeding to pig is delivered for sale, statistics infection morbidity number (fever, abdomen
The infectious diseases summation such as rush down), the death rate and average body weight growth rate (average weight incrementss/initially average weight * 100%), tool
Body the results are shown in Table 2.
Table 2
| Test item | Infection morbidity number | The death rate | Average weight growth rate |
| Blank control group | 9 | 5% | 200.6% |
| Experimental group 1 | 2 | 0% | 289.5% |
| Experimental group 2 | 1 | 0% | 297.8% |
| Experimental group 3 | 0 | 0% | 308.1% |
| Experimental group 4 | 1 | 0% | 302.5% |
| Experimental group 5 | 0 | 0% | 305.6% |
| Control group 1 | 3 | 1% | 266.7% |
| Control group 2 | 5 | 2% | 248.5% |
| Control group 3 | 5 | 2% | 256.2% |
| Control group 4 | 4 | 1% | 252.8% |
As shown in Table 2, feed addictive of the invention can improve the effect of piglet anti-infection ability, and for growing state
Also it has some improvement, and is substantially better than control group 1-4, and by control group 1-4 it is found that by the way that female is added in feed addictive
Loyal sub- extract, Chinese herbal medicine extract, Cordyceps Militaris solid fermentation culture and enzymatic hydrolysis pine needle meal, can improve piglet anti-infection ability
Effect effect, and promote the growth of piglet, and interact between above-mentioned four kinds of ingredients, have the effect of collaboration, enhancing.
To sum up, the present invention provides a kind of feed addictive based on antibacterial peptide, can be by being equipped with female on the basis of antibacterial peptide
The ingredients such as loyal sub- extract, Chinese herbal medicine extract, Cordyceps Militaris solid fermentation culture, enzymatic hydrolysis pine needle meal, are assisted by each ingredient
Same synergistic effect improves the immunity of livestock and poultry to improve feed addictive antibacterial ability of the invention, enhances the body of livestock and poultry
Matter, and livestock and poultry can be improved to the utilization efficiency of feed, accelerate its speed of growth, to improve raise benefit.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, although referring to aforementioned reality
Applying example, invention is explained in detail, for those skilled in the art, still can be to aforementioned each implementation
Technical solution documented by example is modified or equivalent replacement of some of the technical features.It is all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (8)
1. a kind of feed addictive based on antibacterial peptide, which is characterized in that the feed addictive includes: according to parts by weight
10-15 parts of antibacterial peptide, 6-12 parts of fruit of glossy privet extract, 5-10 parts of Chinese herbal medicine extract, Cordyceps Militaris solid fermentation culture
3-5 parts, enzymatic hydrolysis pine needle meal 2-4 part, 4-7 parts of soya-bean polypeptides, 0.5-1.5 parts of Se-enriched yeast powder, 0.4-0.8 parts of cellulase, answer
Close amino acid 0.5-1 parts, 0.3-0.5 parts of lysine chelated copper；
Wherein, the Chinese herbal medicine extract is Shorthorned Epimedium P.E, Astragalus Root P.E and wrinkled giant hyssop leaf extract 3:2:1 in mass ratio
It mixes.
2. the feed addictive according to claim 1 based on antibacterial peptide, which is characterized in that the feed addictive is by weight
Amount number meter includes: 12.5 parts of antibacterial peptide, 9 parts of fruit of glossy privet extract, 7.5 parts of Chinese herbal medicine extract, the training of cordyceps sinensis bacteria solid fermentation
Support 4 parts of object, enzymatic hydrolysis 3 parts of pine needle meal, 5.5 parts of soya-bean polypeptides, 1 part of Se-enriched yeast powder, 0.6 part of cellulase, compound amino acid 0.7
Part, 0.4 part of lysine chelated copper.
3. the feed addictive according to claim 1 based on antibacterial peptide, which is characterized in that the Shorthorned Epimedium P.E
The preparation method comprises the following steps: 8 times of purified waters of Herba Epimedii first are added to be ground into coarse grain after drinking water cleaning, drying in Herba Epimedii, 2 are decocted
It is secondary, it decocts 2 hours every time, merges decoction liquor, being concentrated into relative density is 1.15 medicinal extract, is let cool, and 95% ethyl alcohol is added and carries out alcohol
It is heavy, 24 hours are stood, supernatant is extracted, is concentrated, being concentrated into relative density is 1.30, and medicinal extract is put into rail mounted film and is done
Dry machine, is dried, and after dry, is ground into fine powder to get Shorthorned Epimedium P.E；
The Astragalus Root P.E the preparation method comprises the following steps: Radix Astragali first crushed 40-50 mesh after, addition Radix Astragali 8-10 times of weight
50% ethanol solution is used 40-50kHz ultrasonic wave assisted extraction 2 times, each 30min under room temperature, filters to obtain ethyl alcohol extraction
Liquid adds 5-8 times of weight hot water in the residue after extraction, and 80 DEG C of decoction 1h, solution is mixed with ethanol extract after filtering, and 100
DEG C straight fire heating concentrated extracting solution, conventional method are dry；60 meshes be crushed to get Astragalus Root P.E；
The wrinkled giant hyssop leaf extract the preparation method comprises the following steps: after Radix agastaches are crushed be added 5-8 times of pure water, with corase grinding grind, and add
Enter pueraria lobata, additional amount is ground after mixing through colloid mill with the 1-2% of wrinkled giant hyssop weight, adjusts the gap of colloid mill stator and rotor
It is 50-100 microns, colloid mill flow control is 0.2-0.4 ton/hours, is added in the 0.05-0.2% of slurry weight later
Property protease digested, pH6.8-7.2,55-60 DEG C of temperature, enzymolysis time 45-55min, enzymolysis liquid enzyme deactivation, filtering, freezing
It crushes after concentration, freeze-drying to get wrinkled giant hyssop leaf extract.
4. the feed addictive according to claim 1 based on antibacterial peptide, which is characterized in that the cordyceps sinensis bacteria solid fermentation
Culture the preparation method comprises the following steps:
(1) Cordyceps Militaris activation culture: the Cordyceps Militaris of preservation is transferred on culture medium, 23-27 DEG C of culture 40-45h, until mycelia is long
Full culture medium；
(2) seed culture: by one piece of above-mentioned cordyceps species picking access equipped with 100 milliliters of culture mediums 500 milliliters of shaking flasks in into
Row seed culture, condition of culture: 100-140 revs/min of rotary shaker, 23-27 DEG C of culture 60-70h；
(3) fermented and cultured: shake-flask seed is consolidated with the access of 5-10% inoculum concentration equipped with the 500L of solid fermentation culture medium after sterilizing
In body fermentor, condition of culture: charge 45%, incubation time 20-30 days, was opened every 2-5 days by 22-26 DEG C of cultivation temperature
Stir 5min；
(4) fermented and cultured terminates, by dry materials to moisture content 5% hereinafter, carrying out crushed 40- to solid fermentation material
60 meshes.
5. the feed addictive according to claim 4 based on antibacterial peptide, which is characterized in that solid used in step (4)
The carbon source selection of fermentation medium is spent certainly from corncob after wheat bran, glucose, sucrose, starch, corn flour, crushing, nitrogen source selection
Raw cake powder, peptone, De-fatted soya protein Powder, beancake powder, yeast powder need inorganic salts to be added to have potassium dihydrogen phosphate, sulfuric acid
Magnesium.
6. the feed addictive according to claim 1 based on antibacterial peptide, which is characterized in that the system of the enzymatic hydrolysis pine needle meal
Preparation Method are as follows: pine needle meal is placed in material-compound tank, the pure water of 2-4 times of pine needle meal weight is added, and medium temperature amylase, enzyme activity is added
It is the protease of 30u/g corn flour with enzyme activity for 30u/g corn flour, warming while stirring to 45-55 DEG C of heat preservation 20-25min,
Then it is to slowly warm up to 45-50 DEG C of heat preservation 20-30min, freeze-drying is to get enzymatic hydrolysis pine needle meal.
7. the feed addictive according to claim 1 based on antibacterial peptide, which is characterized in that the compound amino acid is under
The group for stating weight percent meter is grouped as: lysine 42%, methionine 44% and threonine 14%.
8. the preparation process of the feed addictive according to claim 1-7 based on antibacterial peptide, which is characterized in that
The following steps are included:
(1) by fruit of glossy privet extract, Chinese herbal medicine extract, Cordyceps Militaris solid fermentation culture and enzymatic hydrolysis pine needle meal drying, drying
Temperature is 65 DEG C, is crushed after drying, crosses 50 meshes, obtains mixed powder；
(2) above-mentioned mixture is added in agitator tank, the water of mixed powder weight 25% is added, stirs evenly, access antibacterial peptide and big
Beans polypeptide, the ultrasonic disperse 10-15min at a temperature of 40-50 DEG C, obtains mixed serum；
(4) Se-enriched yeast powder, cellulase, compound amino acid, lysine chelated copper are added in Xiang Shangshu mixed serum, mixing is equal
It is even, it then puts into hardening and tempering furnace, is passed through the quenched 8min of 10% vapor that temperature is 85 DEG C, quality is each component gross mass,
Then make material 95-105 DEG C at a temperature of enter pelletizer in pelletize；
(5) the feed addictive particle after granulation is dried to get the feed addictive based on antibacterial peptide.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030056244A1 (en) * | 2000-05-02 | 2003-03-20 | Ning Huang | Feed additive compositions and methods |
| CN102657287A (en) * | 2012-06-05 | 2012-09-12 | 荆州双胞胎饲料有限公司 | Compound feed addictive with bacteria and oxidation resisting functions |
| CN102696884A (en) * | 2012-06-05 | 2012-10-03 | 邵素英 | Composite feed additive product |
| CN105146103A (en) * | 2015-09-17 | 2015-12-16 | 天津中天精科科技有限公司 | Feed additive |
| CN105166410A (en) * | 2015-09-17 | 2015-12-23 | 邵素英 | Feed additive with good thermal stability |
-
2019
- 2019-03-28 CN CN201910241172.0A patent/CN109874909A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030056244A1 (en) * | 2000-05-02 | 2003-03-20 | Ning Huang | Feed additive compositions and methods |
| CN102657287A (en) * | 2012-06-05 | 2012-09-12 | 荆州双胞胎饲料有限公司 | Compound feed addictive with bacteria and oxidation resisting functions |
| CN102696884A (en) * | 2012-06-05 | 2012-10-03 | 邵素英 | Composite feed additive product |
| CN105146103A (en) * | 2015-09-17 | 2015-12-16 | 天津中天精科科技有限公司 | Feed additive |
| CN105166410A (en) * | 2015-09-17 | 2015-12-23 | 邵素英 | Feed additive with good thermal stability |
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