CN109837315B - 用于生产目标物质的方法 - Google Patents

用于生产目标物质的方法 Download PDF

Info

Publication number
CN109837315B
CN109837315B CN201811441142.6A CN201811441142A CN109837315B CN 109837315 B CN109837315 B CN 109837315B CN 201811441142 A CN201811441142 A CN 201811441142A CN 109837315 B CN109837315 B CN 109837315B
Authority
CN
China
Prior art keywords
gene
activity
microorganism
strain
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201811441142.6A
Other languages
English (en)
Chinese (zh)
Other versions
CN109837315A (zh
Inventor
B.米杰茨
C.罗奇
R.穆拉里
朝里彩香
福井启太
户矢崎未来
野口景子
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
Original Assignee
Ajinomoto Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc
Publication of CN109837315A publication Critical patent/CN109837315A/zh
Application granted granted Critical
Publication of CN109837315B publication Critical patent/CN109837315B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1014—Hydroxymethyl-, formyl-transferases (2.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/12—Methionine; Cysteine; Cystine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/46—Streptococcus ; Enterococcus; Lactococcus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y105/00—Oxidoreductases acting on the CH-NH group of donors (1.5)
    • C12Y105/01—Oxidoreductases acting on the CH-NH group of donors (1.5) with NAD+ or NADP+ as acceptor (1.5.1)
    • C12Y105/0102—Methylenetetrahydrofolate reductase [NAD(P)H] (1.5.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/02—Hydroxymethyl-, formyl- and related transferases (2.1.2)
    • C12Y201/02002—Phosphoribosylglycinamide formyltransferase (2.1.2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01006—Methionine adenosyltransferase (2.5.1.6), i.e. adenosylmethionine synthetase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201811441142.6A 2017-11-29 2018-11-29 用于生产目标物质的方法 Active CN109837315B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762591910P 2017-11-29 2017-11-29
US62/591,910 2017-11-29

Publications (2)

Publication Number Publication Date
CN109837315A CN109837315A (zh) 2019-06-04
CN109837315B true CN109837315B (zh) 2024-05-14

Family

ID=64559545

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201811441142.6A Active CN109837315B (zh) 2017-11-29 2018-11-29 用于生产目标物质的方法

Country Status (4)

Country Link
US (1) US11680279B2 (https=)
EP (1) EP3502263B1 (https=)
JP (1) JP7318199B2 (https=)
CN (1) CN109837315B (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10858676B2 (en) * 2017-05-22 2020-12-08 Ajinomoto Co., Inc. Method for producing objective substance
US11680279B2 (en) * 2017-11-29 2023-06-20 Ajinomoto Co., Inc. Method for producing objective substance
CN115243561A (zh) 2020-03-04 2022-10-25 味之素株式会社 突变型转谷氨酰胺酶
MX2023002999A (es) * 2020-09-15 2023-04-10 Amyris Inc Composiciones de cultivo y metodos de su uso para la produccion de vainillina de alto rendimiento.
KR102281367B1 (ko) * 2021-01-26 2021-07-22 씨제이제일제당 (주) 신규한 5,10-메틸렌테트라하이드로폴레이트 리덕타제 변이체 및 이를 이용한 l-발린 생산 방법
US20220275405A1 (en) * 2021-02-26 2022-09-01 Northwestern University Production of vanillin-glucoside from lignin-derived carbon
CN113528411B (zh) * 2021-06-29 2024-07-09 河北维达康生物科技有限公司 生产n-乙酰基-5-甲氧基色胺的基因工程菌、构建方法及应用
WO2023183543A1 (en) * 2022-03-25 2023-09-28 The Trustees Of Princeton University Chemoenzymatic synthesis of selenoneine and its analogs
KR20240086804A (ko) * 2022-12-08 2024-06-19 씨제이제일제당 (주) 퓨린 뉴클레오티드를 생산하는 미생물 및 이를 이용한 퓨린 뉴클레오티드의 생산방법
CN116463306B (zh) * 2023-06-16 2023-08-22 北京量维生物科技研究院有限公司 莽草酸脱氢酶突变体及利用其生产莽草酸的方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5128253A (en) * 1991-05-31 1992-07-07 Kraft General Foods, Inc. Bioconversion process for the production of vanillin
WO2004024931A2 (de) * 2002-08-27 2004-03-25 Basf Aktiengesellschaft Verfahren zur fermentativen herstellung schwefelhaltiger feinchemikalien (metf)
JP2009501550A (ja) * 2005-07-18 2009-01-22 ビーエーエスエフ ソシエタス・ヨーロピア メチオニン生産組換え微生物
WO2014106189A2 (en) * 2012-12-31 2014-07-03 Conagen Inc. Methods of making vanillin via microbial fermentation utilizing ferulic acid provided by a modified caffeic acid 3-o-methyltransferase
WO2015032911A1 (en) * 2013-09-05 2015-03-12 Danmarks Tekniske Universitet Microorganisms for efficient production of melatonin and related compounds
WO2017073701A2 (en) * 2015-10-27 2017-05-04 Ajinomoto Co., Inc. Method for producing aldehyde

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5089592A (https=) 1973-12-13 1975-07-18
JPS57134500A (en) 1981-02-12 1982-08-19 Kyowa Hakko Kogyo Co Ltd Plasmid pcg1
JPS57183799A (en) 1981-04-17 1982-11-12 Kyowa Hakko Kogyo Co Ltd Novel plasmid
JPS5835197A (ja) 1981-08-26 1983-03-01 Kyowa Hakko Kogyo Co Ltd プラスミドｐｃｇ２
JPS58192900A (ja) 1982-05-04 1983-11-10 Ajinomoto Co Inc 複合プラスミド
JPH01191686A (ja) 1988-01-26 1989-08-01 Mitsubishi Petrochem Co Ltd 複合プラスミド
FR2627508B1 (fr) 1988-02-22 1990-10-05 Eurolysine Procede pour l'integration d'un gene choisi sur le chromosome d'une bacterie et bacterie obtenue par ledit procede
US5185262A (en) 1988-07-27 1993-02-09 Mitsubishi Petrochemical Co., Ltd. DNA fragment containing gene which encodes the function of stabilizing plasmid in host microorganism
JP2678995B2 (ja) 1988-09-08 1997-11-19 三菱化学株式会社 トリプトフアンシンターゼの製造法
JPH02207791A (ja) 1989-02-07 1990-08-17 Ajinomoto Co Inc 微生物の形質転換法
JP2973446B2 (ja) 1990-01-11 1999-11-08 三菱化学株式会社 新規プラスミドベクター
JPH07108228B2 (ja) 1990-10-15 1995-11-22 味の素株式会社 温度感受性プラスミド
JPH05244941A (ja) 1992-03-03 1993-09-24 Toyo Ink Mfg Co Ltd Ｎａｄｈ依存性パラヒドロキシ安息香酸ヒドロキシラーゼ及びその製造方法
US5272073A (en) 1992-06-30 1993-12-21 Purdue Research Foundation Biocatalytic synthesis of catechol from glucose
JPH0775589A (ja) 1993-09-08 1995-03-20 Kawasaki Steel Corp プロトカテキュ酸の製造方法
AU4431196A (en) 1995-01-23 1996-08-14 Novo Nordisk A/S Dna integration by transposition
JPH0970291A (ja) 1995-06-30 1997-03-18 Ajinomoto Co Inc 人工トランスポゾンを用いた遺伝子増幅方法
JP4168463B2 (ja) 1996-12-05 2008-10-22 味の素株式会社 Ｌ−リジンの製造法
AU756507B2 (en) 1998-03-18 2003-01-16 Ajinomoto Co., Inc. L-glutamic acid-producing bacterium and method for producing L-glutamic acid
WO2000017319A2 (en) 1998-09-18 2000-03-30 Board Of Trustees Operating Michigan State University Synthesis of vanillin from a carbon source
WO2000018935A1 (en) 1998-09-25 2000-04-06 Ajinomoto Co.,Inc. Process for constructing amino acid-producing bacterium and process for producing amino acid by fermentation method with the use of the thus constructed amino acid-producing bacterium
JP2000262288A (ja) 1999-03-16 2000-09-26 Ajinomoto Co Inc コリネ型細菌の温度感受性プラスミド
WO2004111254A1 (en) 2003-06-19 2004-12-23 Poalis A/S A method of producing a low molecular weight organic compound in a cell
DK1651758T3 (da) 2003-07-29 2008-09-01 Ajinomoto Kk Fremgangsmåde tl fremstilling af L-lysin eller L-threonin ved anvendelse af Escherichia bakterier med svækket æblesyreenzymaktivitet
US20070026505A1 (en) * 2005-06-17 2007-02-01 Madden Kevin T Amino acid and metabolite biosynthesis
JP4882063B2 (ja) 2005-10-12 2012-02-22 国立大学法人東京農工大学 テレフタル酸の代謝に関与する新規遺伝子
JP5088136B2 (ja) 2005-10-18 2012-12-05 味の素株式会社 コハク酸の製造方法
RU2418069C2 (ru) 2006-09-29 2011-05-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) Способ конструирования рекомбинантных бактерий, принадлежащих к роду pantoea, и способ продукции l-аминокислот с использованием бактерий, принадлежащих к роду pantoea
CN105063104B (zh) * 2011-08-08 2019-08-13 国际香料香精公司 用于香草醛或香草醛β-D-葡萄糖苷的生物合成的组合物和方法
BR112013016373B1 (pt) 2011-11-11 2021-05-18 Ajinomoto Co., Inc método para produzir uma substância alvo
AU2013339881B2 (en) 2012-11-05 2017-08-24 Danstar Ferment Ag Vanillin synthase
US10167490B2 (en) * 2012-12-21 2019-01-01 Ergo Health Llc Ergothioneine production through metabolic engineering
US11680279B2 (en) * 2017-11-29 2023-06-20 Ajinomoto Co., Inc. Method for producing objective substance

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5128253A (en) * 1991-05-31 1992-07-07 Kraft General Foods, Inc. Bioconversion process for the production of vanillin
WO2004024931A2 (de) * 2002-08-27 2004-03-25 Basf Aktiengesellschaft Verfahren zur fermentativen herstellung schwefelhaltiger feinchemikalien (metf)
JP2009501550A (ja) * 2005-07-18 2009-01-22 ビーエーエスエフ ソシエタス・ヨーロピア メチオニン生産組換え微生物
WO2014106189A2 (en) * 2012-12-31 2014-07-03 Conagen Inc. Methods of making vanillin via microbial fermentation utilizing ferulic acid provided by a modified caffeic acid 3-o-methyltransferase
WO2015032911A1 (en) * 2013-09-05 2015-03-12 Danmarks Tekniske Universitet Microorganisms for efficient production of melatonin and related compounds
WO2017073701A2 (en) * 2015-10-27 2017-05-04 Ajinomoto Co., Inc. Method for producing aldehyde

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Kunjapur AM, et al..Deregulation of S-adenosylmethionine biosynthesis and regeneration improves methylation in the E. coli de novo vanillin biosynthesis pathway..《Microb Cell Fact》.2016,第15卷(第61期),摘要，表1、2，图1. *
UniProtKB.Full=S-adenosylmethionine synthase，UniProtKB/Swiss-Prot: Q9K5E4.1.《UniProtKB》.2000,序列表. *
曹晨琦.S-腺苷蛋氨酸合成酶高通量筛选及定向进化研究.《中国优秀硕士学位论文全文数据库 基础科学辑》.2023,全文. *

Also Published As

Publication number Publication date
EP3502263A3 (en) 2019-10-16
EP3502263B1 (en) 2021-04-21
US11680279B2 (en) 2023-06-20
EP3502263A2 (en) 2019-06-26
JP2019134700A (ja) 2019-08-15
US20190161776A1 (en) 2019-05-30
CN109837315A (zh) 2019-06-04
JP7318199B2 (ja) 2023-08-01

Similar Documents

Publication Publication Date Title
CN109837315B (zh) 用于生产目标物质的方法
ES2706323T3 (es) Método para producir L-aminoácido de la familia de glutamato
CN109890972B (zh) 生产目标物质的方法
CN109890960B (zh) 用于生产醛的方法
CN109937257B (zh) 生产目标物质的方法
EP2054500B1 (en) An l-glutamic acid producing bacterium and a method for producing l-glutamic acid
JP6881448B2 (ja) アルデヒドの製造方法
KR102074841B1 (ko) 피드백-내성 알파-이소프로필말레이트 신타제
US10883124B2 (en) Method for producing objective substance
CN108929886B (zh) 生产目标物质的方法
BR112016008830B1 (pt) Método para produzir uma substância alvo
WO2017213142A1 (ja) ヒポタウリンまたはタウリンの製造法
KR20190025969A (ko) 당 포스포트랜스퍼라제 시스템 (pts)을 코딩하는 유전자를 포함하는 미생물에 의한 관심 분자의 발효적 생산을 위한 방법
KR20230092008A (ko) L-아미노산의 제조법
WO2020027251A1 (en) Method for producing objective substance
WO2007024011A1 (ja) Ｌ−グルタミン酸生産菌及びｌ−グルタミン酸の製造方法
JP2023001394A (ja) バニリンの製造方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant