CN109836277A - A kind of microbial-bacterial fertilizer combines the method for fermented liquid inhibition tobacco black shank - Google Patents
A kind of microbial-bacterial fertilizer combines the method for fermented liquid inhibition tobacco black shank Download PDFInfo
- Publication number
- CN109836277A CN109836277A CN201910181752.5A CN201910181752A CN109836277A CN 109836277 A CN109836277 A CN 109836277A CN 201910181752 A CN201910181752 A CN 201910181752A CN 109836277 A CN109836277 A CN 109836277A
- Authority
- CN
- China
- Prior art keywords
- bacillus cereus
- black shank
- liquid
- microbial
- tobacco black
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of microbial-bacterial fertilizers to combine the method that the fermented liquid inhibits tobacco black shank, comprising the following steps: (1) prepares zymocyte liquid, (2) prepare bacterial manure, (3) application base fertilizer, (4) pouring zymocyte liquid；The present invention prepares zymocyte liquid and bacterial manure using bacillus cereus (Bacillus cereus), and it uses the method for bacterium solution and bacterial manure drug combination, apply bacillus cereus in the form of solid-state and liquid, bacillus cereus is promoted to be colonized and ultimately form dominant microflora in the soil, the two organically combines to play preferable preventive effect to tobacco black shank.The present invention reduces the use of chemical fertilizer and chemical pesticide, the growth of tobacco is protected and promoted, is promoted environmental-friendly.
Description
Technical field
The invention belongs to biological pesticide technical fields, and in particular to raw using bacillus cereus (Bacilluscereus)
The bacterial manure of production combines the fermented liquid and inhibits tobacco black shank (Phytophthora parasitica var.nicotiane)
Occur.
Background technique
Tobacco black shank (Phytophthora parasitica var.nicotiane) is the destructive disease of tobacco,
It is simultaneously also to endanger one of most serious disease in World tobacco production, which, which respectively produces cigarette province in China, different degrees of hair
It is raw, and endanger more universal and serious.In recent years since continuous cropping tobacco field area expands and the growth of the continuous cropping time limit, many places
Tobacco black shank has the tendency that aggravating popular.People once try out cultural control, change for many years in order to mitigate loss caused by the disease
It learns a variety of methods such as prevention and treatment, breeding for disease resistance and controls tobacco black shank, but since the zoospore of the germ forms speed fastly and number
Amount is big, infects short incubation period, rapid onset after cigarette strain, can occur repeatedly to infect again in a season of growth, while the disease is sometimes
Generation also is mixed with other diseases, so as to cause the forfeiture of some anti-balck shank varietal resistances；Moreover, the large area of disease-resistant variety
Single cropping in flakes year after year, layout is unreasonable, easily loses the resistance of excellent variety, shortens the service life of disease-resistant variety, and
And it is difficult to control if plant disease epidemic.Currently, being used to prevent and treat the chemical agent of tobacco black shank in production is mostly phenyl amide
Class and aliette etc. be same or the identical several systemic fungicides of mechanism of action, it is easy to drug resistance is generated, so as to cause
Drug effect decline, dosing increase.Tobacco is leaf with industrial crops, and appearance and inherent quality to tobacco leaf have special requirement,
Pesticide residue, pathogenic bacteria resistance to drugs and the influence to cigarette health greatly limit the extensive utilization of pesticide.In tobacco and
Under the trend that tobacco product gradually develops to nuisanceless direction, biological control is as a kind of safe and effective method and increasingly
It is taken seriously.
The antagonistic bacterium of tobacco black shank bacterium has Pseudomonas alba (Pseudomonas spp.) and bacillus (Ba-
Cillus spp.), it mainly makes tobacco black shank bacterium myceliolysis by generating antibiotic；By adding in field soil
Enter that antagonistic bacterium discovery can significantly reduce tobacco black shank bacterium infects chance, to reduce disease incidence.It is currently used main
Exactly concentrated liquid preparation, pure spore pulvis or complex solid pulvis is made in Antagonistic Fungi by method, then before tobacco seedlings transplanting
Application.These methods are more single, since biocontrol bacterial strain is influenced by complicated soil ecological factor, biocontrol agent preventive effect it is steady
Qualitative to cannot be guaranteed, preventive effect is unobvious.And it is of the present invention be " drug combination " method, first pass through base fertilizer application,
So that biocontrol microorganisms is colonized in the soil by repeatedly spraying afterwards and ultimately form dominant microflora, to be risen to tobacco black shank
To preferable preventive effect.
Summary of the invention
Tobacco black shank technology is prevented and treated not using single drip irrigation (liquid) the purpose of the present invention is to solve existing
Foot.A kind of new technology that the disease antagonism bacterial manure is combined to prevention and control disease with drip irrigation is provided.It can be mentioned by synergy
The colonization ability of high Antagonistic Fungi in the soil, effectively improves the resistance of the disease of its pair.To tobacco field microenvironment is improved, promote life
The development of object Prevention Technique is of great significance.
In order to achieve the above object, the invention adopts the following technical scheme:
1, zymocyte liquid is prepared
The bacillus cereus (Bacillus cereus) of solid conservation is produced gemma fluid nutrient medium using improvement to carry out
Fermented and cultured obtains zymocyte liquid.Living bacteria count >=1.0 hundred million/g (cfu) in the zymocyte liquid.The improvement produces gemma liquid
Body medium component is as follows: glucose 1.5%, malt extract 2.5%, peptone 0.2%, NaCl 0.1%, (NH4)2SO40.1%, anhydrous CaCl20.03%, ZnSO4·7H2O 0.02%, FeCl3·6H2O 0.002%, H2O 1000m1。
The fluid nutrient medium and zymocyte liquid are prepared by the following method: being weighed said components respectively and be dissolved in 1L steaming
In distilled water, with pH meter pH to 6.5~7.2 is adjusted after to be dissolved, by above-mentioned solution loaded on being placed in autoclave in 121 in triangular flask
DEG C sterilizing 20 minutes, 1ml bacillus cereus was picked with oese after culture medium is cooling and is transferred in the culture medium, in 37 DEG C
Shaking table 100rpm shaken cultivation 48hr.
2, bacterial manure is prepared
The bio-bacterial manure containing bacillus cereus, the side of double secondary stacking fermentations are prepared using the method for double secondary stacking fermentations
Method are as follows: for the first time ferment: by percentage to the quality, take 40~50% peat, 10~20% oil cake, 10~15% flower
Slag, 20~25% carbon ammonium hydroxide and 1~5% zymocyte liquid containing bacillus cereus are first uniformly mixed peat, oil
Withered, flower slag, carbon ammonium hydroxide, are added the water of total volume 10%, and every the turning of progress in 5 days, the stacking time is 40 days；Second of hair
Ferment: for the first time after fermentation, being added the zymocyte liquid containing bacillus cereus, every the turning of progress in 5 days, in favor of
Logical oxygen and moisture evaporation, secondary fermentation time are 15 days, and after fermentation, indices reach national organic fertilizer standard to stacking
NY525-2011.The bio-bacterial manure effective active bacterium >=0.5 hundred million/g (cfu).
3, base fertilizer is applied
The biological organic fertilizer for containing the bacillus cereus described in step 2 is applied as base fertilizer, every pool applies 90 grams, often
100 kilograms of mu application.
4, zymocyte liquid is poured
Bacillus cereus fermentation bacterium solution described in step 1 is diluted according to 5000 times while carrying out step 3, to pour
Fill cigarette strain.
Pour cigarette strain method particularly includes: pour cigarette strain root after cigarette strain transplanting, dosage is 10kg per acre.It is raw in cigarette strain
Long Seedling Stage, than it is sparse when using pour, to cigarette strain Later growth, when tobacco leaf is dense using sprinkling irrigation.It is sprayed using spray irrigation system
It applies on cigarette strain blade face, leaf portion and root spray be intended to be impregnated with every time, spray 1 time within every 15 days, spray every month 2 times.
In the plantation of tobacco, the bacillus cereus bacterial manure in the application present invention can reduce the dosage of chemical fertilizer, waxy
Bacillus contains a large amount of nitrogen-fixing bacteria after being proliferated again, can greatly improve the middle micronutrient levels in soil, reduces nitrogen
The amount of application of phosphorus potassium and other middle microelements；The high-efficiency activated beneficial microbe bacterium containing there are many simultaneously, increases the soil organism,
Accelerate organic matter degradation to be converted into the absorbent nutriment of crop, greatly improve soil fertility, reduces fertilizer amount.Improve cigarette
The quality of grass, increases income tobacco.The soil of also restructural health, improve tobacco resist pest and disease damage, while it is nontoxic, harmless, without dirt
Dye.
In addition, Bacillus cereus fermentation liquid can also play preferable preventive effect to tobacco black shank, it is possible to reduce tobacco
The generation of tobacco black shank in plantation.
For the present invention using the method for double stacking fermentations, partial material can occur for the fermentation of first time stacking can not be all decomposed
The case where, after for the first fermentation, zymocyte liquid is added, bacterium preferably acts in completely not decomposed material, can be quick
Breeding makes material reach harmless treatment (killing germ, worm's ovum, weed seed).Abundant decomposed material has bacterial manure more
Nutriment.
Second of stacking fermentation addition zymocyte liquid can kill virus, germ, worm's ovum, weed seed in material etc.,
Pest and disease damage effectively in containment soil occurs.Material after zymocyte liquid sufficiently ferments does not burn offspring without different smell, and
Nitrogen fixing capacity is strong, can increase soil nutrient availability, promotes plant absorption.Increase the activity of soil beneficial microbe, makes its generation
Biological regulation material incentive root growth and promotion Nutrient Absorption, the effective absorption rate for improving nutriment.
And it is of the present invention be " drug combination " method, apply bacillus cereus in the form of solid-state and liquid
With promotion bacillus cereus is colonized in the soil and ultimately forms dominant microflora, and the two organically combines to the black shin of tobacco
Disease plays preferable preventive effect.The present invention reduces the use of chemical fertilizer and chemical pesticide, the growth of tobacco is protected and promotes,
It does not cause tobacco to accumulate harmful substance, has no adverse effects to ecological environment, while can also gradually improve the ecology of planting soil
Environment, to global environment close friend.
Detailed description of the invention
Fig. 1 is bacillus cereus (Bacillus cereus) bacterium colony figure in the present invention；
Fig. 2 is bacillus cereus (Bacillus cereus) cellular morphology figure in the present invention.
Specific embodiment
The present invention will be further explained with reference to the examples below, as described below, is only to preferable implementation of the invention
Example, not limits the present invention, any person skilled in the art is possibly also with the disclosure above
Technology contents changed or be modified as the equivalent embodiment changed on an equal basis.Without departing from the concept of the present invention, foundation
Technical spirit any simple modification, equivalent change and modification made to the above embodiment of the invention, all fall within of the invention
In protection scope.
Embodiment 1
Bacillus cereus (Bacillus cereus) in the present invention has following biological characteristics: training in LB solid
Bacterium colony protrusion on base is supported, the smooth of the edge is fine and smooth, it is creamy white, diameter 1.1mm~1.8mm.See Fig. 1, Fig. 2.
1, zymocyte liquid is prepared
The bacillus cereus (Bacillus cereus) of solid conservation is produced gemma fluid nutrient medium using improvement to carry out
Fermented and cultured obtains zymocyte liquid.Living bacteria count >=1.0 hundred million/g (cfu) in the zymocyte liquid.The improvement produces gemma liquid
Body medium component is as follows: glucose 1.5%, malt extract 2.5%, peptone 0.2%, NaCl 0.1%, (NH4)2SO40.1%, anhydrous CaCl20.03%, ZnSO4·7H2O 0.02%, FeCl3·6H2O 0.002%, H2O 1000m1。
The fluid nutrient medium and zymocyte liquid are prepared by the following method: being weighed said components respectively and be dissolved in 1L distillation
In water, with pH meter pH to 6.5 is adjusted after to be dissolved, above-mentioned solution is sterilized in 121 DEG C loaded on being placed in autoclave in triangular flask
20 minutes, 1ml bacillus cereus was picked with oese after culture medium is cooling and is transferred in the culture medium, in 37 DEG C of shaking tables
100rpm shaken cultivation 48hr.
2, bacterial manure is prepared
The bio-bacterial manure containing bacillus cereus, the side of double secondary stacking fermentations are prepared using the method for double secondary stacking fermentations
Method are as follows: ferment for the first time: by percentage to the quality, taking 40% peat, 20% oil cake, 15% flower slag, 23% carbon ammonia
Water and 2% the zymocyte liquid containing bacillus cereus, be first uniformly mixed peat, oil cake, flower slag, carbon ammonium hydroxide, be added total
The water of volume 10%, every the turning of progress in 5 days, the stacking time is 40 days；Second of fermentation: for the first time after fermentation,
The zymocyte liquid containing bacillus cereus is added, it is secondary in favor of logical oxygen and moisture evaporation every the turning of progress in 5 days
Fermentation time is 15 days, and after fermentation, indices reach national organic fertilizer standard NY525-2011 to stacking.The biological bacteria
Fertile effective active bacterium >=0.5 hundred million/g (cfu).
3, base fertilizer is applied
The biological organic fertilizer for containing the bacillus cereus described in step 2 is applied as base fertilizer, every pool applies 90 grams, often
100 kilograms of mu application.
4, zymocyte liquid is poured
Bacillus cereus fermentation bacterium solution described in step 1 is diluted according to 5000 times while carrying out step 3, to pour
Fill cigarette strain.
Pour cigarette strain method particularly includes: pour cigarette strain root after cigarette strain transplanting, dosage is 10kg per acre.It is raw in cigarette strain
Long Seedling Stage, than it is sparse when using pour, to cigarette strain Later growth, when tobacco leaf is dense using sprinkling irrigation.It is sprayed using spray irrigation system
It applies on cigarette strain blade face, leaf portion and root spray be intended to be impregnated with every time, spray 1 time within every 15 days, spray every month 2 times.
Embodiment 2
1, zymocyte liquid is prepared
The bacillus cereus (Bacillus cereus) of solid conservation is produced gemma fluid nutrient medium using improvement to carry out
Fermented and cultured obtains zymocyte liquid.Living bacteria count >=1.0 hundred million/g (cfu) in the zymocyte liquid.The improvement produces gemma liquid
Body medium component is as follows: glucose 1.5%, malt extract 2.5%, peptone 0.2%, NaCl 0.1%, (NH4)2SO40.1%, anhydrous CaCl20.03%, ZnSO4·7H2O 0.02%, FeCl3·6H2O 0.002%, H2O 1000m1。
The fluid nutrient medium meter zymocyte liquid is prepared by the following method: being weighed said components respectively and is dissolved in 1L distillation
In water, with pH meter pH to 7.0 is adjusted after to be dissolved, above-mentioned solution is sterilized in 121 DEG C loaded on being placed in autoclave in triangular flask
20 minutes, 1ml bacillus cereus was picked with oese after culture medium is cooling and is transferred in the culture medium, in 37 DEG C of shaking tables
100rpm shaken cultivation 48hr.
3, bacterial manure is prepared
The bio-bacterial manure containing bacillus cereus, the side of double secondary stacking fermentations are prepared using the method for double secondary stacking fermentations
Method are as follows: ferment for the first time: by percentage to the quality, taking 50% peat, 19% oil cake, 10% flower slag, 20% carbon ammonia
Water and 1% the zymocyte liquid containing bacillus cereus, be first uniformly mixed peat, oil cake, flower slag, carbon ammonium hydroxide, be added total
The water of volume 10%, every the turning of progress in 5 days, the stacking time is 40 days；Second of fermentation: for the first time after fermentation,
The zymocyte liquid containing bacillus cereus is added, it is secondary in favor of logical oxygen and moisture evaporation every the turning of progress in 5 days
Fermentation time is 15 days, and after fermentation, indices reach national organic fertilizer standard NY525-2011 to stacking.The biological bacteria
Fertile effective active bacterium >=0.5 hundred million/g (cfu).
3, base fertilizer is applied
The biological organic fertilizer for containing the bacillus cereus described in step 2 is applied as base fertilizer, every pool applies 90 grams, often
100 kilograms of mu application.
4, zymocyte liquid is poured
Bacillus cereus fermentation bacterium solution described in step 1 is diluted according to 5000 times while carrying out step 3, to pour
Fill cigarette strain.
Pour cigarette strain method particularly includes: pour cigarette strain root after cigarette strain transplanting, dosage is 10kg per acre.It is raw in cigarette strain
Long Seedling Stage, than it is sparse when using pour, to cigarette strain Later growth, when tobacco leaf is dense using sprinkling irrigation.It is sprayed using spray irrigation system
It applies on cigarette strain blade face, leaf portion and root spray be intended to be impregnated with every time, spray 1 time within every 15 days, spray every month 2 times.
Embodiment 3
1, zymocyte liquid is prepared
The bacillus cereus (Bacillus cereus) of solid conservation is produced gemma fluid nutrient medium using improvement to carry out
Fermented and cultured obtains zymocyte liquid.Living bacteria count >=1.0 hundred million/g (cfu) in the zymocyte liquid.The improvement produces gemma liquid
Body medium component is as follows: glucose 1.5%, malt extract 2.5%, peptone 0.2%, NaCl 0.1%, (NH4)2SO40.1%, anhydrous CaCl20.03%, ZnSO4·7H2O 0.02%, FeCl3·6H2O 0.002%, H2O 1000m1。
The fluid nutrient medium and zymocyte liquid are prepared by the following method: being weighed said components respectively and be dissolved in 1L distillation
In water, with pH meter pH to 7.2 is adjusted after to be dissolved, above-mentioned solution is sterilized in 121 DEG C loaded on being placed in autoclave in triangular flask
20 minutes, 1ml bacillus cereus was picked with oese after culture medium is cooling and is transferred in the culture medium, in 37 DEG C of shaking tables
100rpm shaken cultivation 48hr.
2, bacterial manure is prepared
The bio-bacterial manure containing bacillus cereus, the side of double secondary stacking fermentations are prepared using the method for double secondary stacking fermentations
Method are as follows: ferment for the first time: by percentage to the quality, taking 48% peat, 10% oil cake, 12% flower slag, 25% carbon ammonia
Water and 5% the zymocyte liquid containing bacillus cereus, be first uniformly mixed peat, oil cake, flower slag, carbon ammonium hydroxide, be added total
The water of volume 10%, every the turning of progress in 5 days, the stacking time is 40 days；Second of fermentation: for the first time after fermentation,
The zymocyte liquid containing bacillus cereus is added, it is secondary in favor of logical oxygen and moisture evaporation every the turning of progress in 5 days
Fermentation time is 15 days, and after fermentation, indices reach national organic fertilizer standard NY525-2011 to stacking.The biological bacteria
Fertile effective active bacterium >=0.5 hundred million/g (cfu).
3, base fertilizer is applied
The biological organic fertilizer for containing the bacillus cereus described in step 2 is applied as base fertilizer, every pool applies 90 grams, often
100 kilograms of mu application.
4, zymocyte liquid is poured
Bacillus cereus fermentation bacterium solution described in step 1 is diluted according to 5000 times while carrying out step 3, to pour
Fill cigarette strain.
Pour cigarette strain method particularly includes: pour cigarette strain root after cigarette strain transplanting, dosage is 10kg per acre.It is raw in cigarette strain
Long Seedling Stage, than it is sparse when using pour, to cigarette strain Later growth, when tobacco leaf is dense using sprinkling irrigation.It is sprayed using spray irrigation system
It applies on cigarette strain blade face, leaf portion and root spray be intended to be impregnated with every time, spray 1 time within every 15 days, spray every month 2 times.
Experimental example
Tobacco black shank efficiency test is carried out in Dongzhou Period in Chuxiong Nanhua testing site, test variety is Hongda tobacco.Examination
Testing area is 4.5 mu, if 2 processing and control group CK, respectively Chemical treatment, the method for the present invention processing (are used and implemented
Method used in example 2) it is each processing 0.5 mu, every processing is repeated 3 times.It is tested according to the method described above, the results showed that change
Learning pesticide-treated tobacco black shank disease incidence is 77.33%, and disease refers to be 43.24；Control group CK tobacco black shank disease incidence is
87.67%, disease refers to be 57.37；Use present invention processing tobacco black shank disease incidence for 69%, disease refers to be 35.59.Statistical analysis
The result shows that preventive effect is obvious with control group CK significant difference after present invention processing.It is shown in Table 1.
1 different disposal tobacco black shank investigation result of table
Claims (7)
1. a kind of microbial-bacterial fertilizer combine the fermented liquid inhibit tobacco black shank method, feature the following steps are included:
(1) it prepares zymocyte liquid: the bacillus cereus (Bacillus cereus) of solid conservation is produced into gemma liquid using improvement
Body culture medium carries out fermented and cultured, obtains the zymocyte liquid containing bacillus cereus.
(2) it prepares bacterial manure: utilizing bio-bacterial manure of the method preparation containing bacillus cereus of double stacking fermentations；
(3) it applies base fertilizer: the bio-bacterial manure for containing the bacillus cereus described in step (2) being applied as base fertilizer, every pool is applied
With 90 grams, 100 kilograms are applied per acre.
(4) it pours zymocyte liquid: containing Bacillus cereus fermentation bacterium solution for what step (1) obtained while carrying out step (3)
It is diluted according to 5000 times, pours cigarette strain.
2. a kind of microbial-bacterial fertilizer according to claim 1 combines the method that the fermented liquid inhibits tobacco black shank,
It is characterized in that: living bacteria count >=1.0 hundred million/g (cfu) in zymocyte liquid in step (1).
3. a kind of microbial-bacterial fertilizer according to claim 1 combines the method that the fermented liquid inhibits tobacco black shank,
Be characterized in that: it is as follows to produce gemma Liquid Culture based component for improvement in step (1): glucose 1.5%, malt extract 2.5%, egg
White peptone 0.2%, NaCl 0.1%, (NH4)2SO40.1%, anhydrous CaCl20.03%, ZnSO4·7H2O 0.02%,
FeCl3·6H2O 0.002%, H2O 1000m1。
4. a kind of microbial-bacterial fertilizer according to claim 1 combines the method that the fermented liquid inhibits tobacco black shank,
It is characterized in that: bio-bacterial manure effective active bacterium >=0.5 hundred million/g (cfu) in step (2).
5. a kind of microbial-bacterial fertilizer according to claim 1 combines the method that the fermented liquid inhibits tobacco black shank,
It is characterized in that: the method for double stacking fermentations in step (2) are as follows: ferment for the first time: by percentage to the quality, taking 40~50%
Peat, 10~20% oil cake, 10~15% flower slag, 20~25% carbon ammonium hydroxide and 1~5% contain waxy gemma bar
The zymocyte liquid of bacterium is first uniformly mixed peat, oil cake, flower slag, carbon ammonium hydroxide, the water of total volume 10% is added, carried out one every 5 days
Secondary turning, stacking time are 40 days；Second of fermentation: for the first time after fermentation, the fermentation containing bacillus cereus is added
Bacterium solution, every the turning of progress in 5 days, the secondary fermentation time is 15 days, and after fermentation, indices reach country to be had stacking
Machine fertilizer standard NY525-2011.
6. a kind of microbial-bacterial fertilizer according to claim 1 combines the method that the fermented liquid inhibits tobacco black shank,
It is characterized in that: pouring cigarette strain in step (4) method particularly includes: pour cigarette strain root after cigarette strain transplanting, dosage is per acre
10kg；Cigarette strain grow Seedling Stage, than it is sparse when using pour, to cigarette strain Later growth, when tobacco leaf is dense using sprinkling irrigation, make
It is sprayed on cigarette strain blade face with spray irrigation system, leaf portion and root spray be intended to be impregnated with every time, spray 1 time within every 15 days, every month
It sprays 2 times.
7. a kind of microbial-bacterial fertilizer according to claim 3 combines the method that the fermented liquid inhibits tobacco black shank,
Be characterized in that: the fluid nutrient medium and zymocyte liquid are prepared by the following method: being weighed said components respectively and be dissolved in 1L
In distilled water, with pH meter adjust pH to 6.5~7.2 after to be dissolved, by above-mentioned solution loaded on be placed in autoclave in triangular flask in
121 DEG C sterilize 20 minutes, pick 1ml bacillus cereus with oese after culture medium is cooling and are transferred in the culture medium, in
37 DEG C of shaking table 100rpm shaken cultivation 48hr.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910181752.5A CN109836277A (en) | 2019-03-11 | 2019-03-11 | A kind of microbial-bacterial fertilizer combines the method for fermented liquid inhibition tobacco black shank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910181752.5A CN109836277A (en) | 2019-03-11 | 2019-03-11 | A kind of microbial-bacterial fertilizer combines the method for fermented liquid inhibition tobacco black shank |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109836277A true CN109836277A (en) | 2019-06-04 |
Family
ID=66885587
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910181752.5A Pending CN109836277A (en) | 2019-03-11 | 2019-03-11 | A kind of microbial-bacterial fertilizer combines the method for fermented liquid inhibition tobacco black shank |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109836277A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999010477A1 (en) * | 1997-08-22 | 1999-03-04 | Agraquest, Inc. | A novel bacillus pumilus strain for controlling corn rootworm, nematode and armyworm infestations |
| CN104230563A (en) * | 2014-09-11 | 2014-12-24 | 红云红河烟草(集团)有限责任公司 | Microbial organic fertilizer containing Bacillus cereus |
-
2019
- 2019-03-11 CN CN201910181752.5A patent/CN109836277A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999010477A1 (en) * | 1997-08-22 | 1999-03-04 | Agraquest, Inc. | A novel bacillus pumilus strain for controlling corn rootworm, nematode and armyworm infestations |
| CN104230563A (en) * | 2014-09-11 | 2014-12-24 | 红云红河烟草(集团)有限责任公司 | Microbial organic fertilizer containing Bacillus cereus |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101124915B (en) | Biocontrol bacteria compound agent for preventing and controlling crop soil-infectious diseases and preparation method | |
| CN107282628A (en) | A kind of composite modifying agent for repairing farmland pollution soil and preparation method thereof | |
| CN105018366B (en) | Methylotrophic bacillus and its application | |
| CN103952329B (en) | Bacillus vallismortis and application thereof | |
| CN102726454B (en) | Biological control agent for fusarium wilt and preparation and use method of biological control agent | |
| CN101875571B (en) | Method for preparing enhanced liquid microbial organic fertilizer | |
| CN105112057B (en) | A kind of heavy metal-polluted soil renovation agent and its preparation and application | |
| CN107619308A (en) | Prevent and treat root-knot nematode biological organic fertilizer and preparation method and application | |
| CN112725213B (en) | Arthrobacter and application thereof as decomposing agent for decomposing vegetable straw | |
| CN109182222A (en) | The method of heavy metal cadmium in selenium-rich microbial agent preparation and its degradation soil and crops | |
| CN106119155A (en) | For preventing and treating bacterial strain WXX 2 and the microbial inoculum of Roots of Peanut maize ear rot | |
| CN107325821A (en) | A kind of biomass carbon base microbe microbial inoculum for preventing and treating watermelon blight and preparation method and application | |
| CN107164262A (en) | A kind of microbial bacterial agent and its bacterial manure available for controlling crop diseases and insect pests | |
| CN107287139A (en) | A kind of microorganism formulation and preparation method thereof and the application in continuous cropping obstacle is improved | |
| CN107410364A (en) | A kind of microbial bacterial agent and its bacterial manure that can be used for preventing and treating root-knot nematode | |
| CN105923711B (en) | A kind of functional water and its functional water dimension stereo agriculture planting method | |
| CN111670769B (en) | Method for improving stress resistance of rice | |
| CN102533603B (en) | Pseudomonas sp. 841P-3 for preventing cotton verticillium wilt and use thereof | |
| CN104671854A (en) | Preparation method of compound photosynthetic bacterial fertilizer | |
| CN103387974A (en) | Preparation method of probiotic microbial inoculum for prevention of genseng replant diseases | |
| CN110150322A (en) | A kind of environmentally protective medicament, preparation method and its application method of prevention and control gray mold | |
| CN109759441A (en) | A kind of alkaline land soil restorative procedure | |
| CN103951483B (en) | A kind of prevent and treat farm crop soil-borne disease biocontrol bacterial fertilizer and production technique and application | |
| CN112522149A (en) | Compound microbial agent capable of preventing and treating tobacco diseases | |
| CN108424182A (en) | A kind of application of biogas slurry concentrate in prevention tomato root-knot eelworm disease |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190604 |
|
| RJ01 | Rejection of invention patent application after publication |