CN104230563A - Microbial organic fertilizer containing Bacillus cereus - Google Patents

Microbial organic fertilizer containing Bacillus cereus Download PDF

Info

Publication number
CN104230563A
CN104230563A CN201410460497.5A CN201410460497A CN104230563A CN 104230563 A CN104230563 A CN 104230563A CN 201410460497 A CN201410460497 A CN 201410460497A CN 104230563 A CN104230563 A CN 104230563A
Authority
CN
China
Prior art keywords
bacillus cereus
organic fertilizer
fertilizer
days
peat
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201410460497.5A
Other languages
Chinese (zh)
Other versions
CN104230563B (en
Inventor
徐洁
王绍坤
周晓罡
王耀进
王超
杨应明
罗华元
朱海滨
姚春馨
丁玉梅
陶南
戴毕
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
YUNNAN QIANZHOU BIOLOGICAL ORGANIC CO Ltd
Hongyun Honghe Tobacco Group Co Ltd
Biotechnology and Germplasm Resource Institute of Yunnan Academy of Agricultural Sciences
Original Assignee
YUNNAN QIANZHOU BIOLOGICAL ORGANIC CO Ltd
Hongyun Honghe Tobacco Group Co Ltd
Biotechnology and Germplasm Resource Institute of Yunnan Academy of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by YUNNAN QIANZHOU BIOLOGICAL ORGANIC CO Ltd, Hongyun Honghe Tobacco Group Co Ltd, Biotechnology and Germplasm Resource Institute of Yunnan Academy of Agricultural Sciences filed Critical YUNNAN QIANZHOU BIOLOGICAL ORGANIC CO Ltd
Priority to CN201410460497.5A priority Critical patent/CN104230563B/en
Publication of CN104230563A publication Critical patent/CN104230563A/en
Application granted granted Critical
Publication of CN104230563B publication Critical patent/CN104230563B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fertilizers (AREA)

Abstract

The invention specifically relates to a microbial organic fertilizer containing Bacillus cereus. The microbial organic fertilizer contains 1%-5% of Bacillus cereus, 35%-40% of smoke residue, 35%-45% of peat, 8%-10% of oil cake and 9%-12% of flower dregs and the microbial organic fertilizer is prepared by uniformly mixing the raw materials, adding water accounting for 10% of total volume and piling for fermentation. The biological organic fertilizer is prepared by turning the pile every 5 days and fermenting for 40 days, and the bacterial fertilizer has not less than 0.05 billion/g (cfu) of effective active bacteria (Bacillus cereus). The biological organic fertilizer is applied as a base fertilizer, and a Bacillus cereus fermentation bacterial solution is simultaneously diluted according to 5000 times, transplanted and then irrigated at the root parts of tobacco plants or spray-applied on foliage, so that tobacco black shank can be effectively prevented and controlled and environmental pollution caused by use of chemical medicaments can be reduced.

Description

A kind of microorganism organic fertilizer containing bacillus cereus
Technical field
The invention belongs to biological pesticide technical field, relate to a kind of bio-organic fertilizer containing bacillus cereus, is exactly specifically the generation microbial-bacterial fertilizer of bacillus cereus production being suppressed black shank in conjunction with this fermented liquid mutual association.
Background technology
Black shank (Phytophthora parasitica var.nicotiane) is the destructive disease of tobacco, also be endanger one of the most serious disease in World tobacco production simultaneously, this disease is respectively produced cigarette in China and is economized the generation all had in various degree, and harm is comparatively general and serious.In recent years due to the growth of continuous cropping vega enlarged areas and the continuous cropping time limit, many local black shanks have and increase the weight of popular trend.The loss that causes to alleviate this disease of people for many years, once the multiple method such as cultural control, chemical prevention, breeding for disease resistance on probation controlled black shank, but the zoospore formation speed due to this germ is fast and quantity is large, infect short incubation period, rapid onset after cigarette strain, can occur repeatedly to infect again a season of growth, this disease sometimes also mixes with other disease and occurs simultaneously, thus causes the forfeiture of some anti-balck shank varietal resistances; And the big area single cropping in flakes year after year of disease-resistant variety, layout is unreasonable, easily makes the resistant lose of improved seeds, shortens the working life of disease-resistant variety, and once plant disease epidemic, is difficult to control.At present, production is used for prevent and treat the chemical agent of black shank and mostly is the same such as phenylamide and phosethyl Al or the identical several systemic fungicides of mechanism of action, be easy to produce resistance, thus cause drug effect to decline, dosing increase.Tobacco is leaf with cash crop, and have special requirement to the outward appearance of tobacco leaf and inner quality, pesticide residue, pathogenic bacteria resistance to drugs and the impact on cigarette health greatly limit the extensive utilization of agricultural chemicals.At tobacco and tobacco product gradually under the trend of nuisanceless future development, biological control more and more comes into one's own as the safe and effective method of one.
The antagonistic bacterium of tobacco black shank bacterium has Pseudomonas alba (Pseudomonas spp.) and genus bacillus (Ba-cillus spp.), and it makes tobacco black shank bacterium myceliolysis mainly through producing antibiotic; Infect chance by what add in field soil that antagonistic bacterium finds significantly to reduce tobacco black shank bacterium, thus reduce sickness rate.Antagonistic Fungi is made concentrated liquid preparation, pure spore pulvis or composite solid pulvis by the main method of current employing exactly, then uses before cigarette transplantation of seedlings.These methods are comparatively single, and because biocontrol strain is subject to the impact of complicated soil ecological factor, the stability of biocontrol fungicide preventive effect can not be guaranteed, and preventive effect is not obvious.And this patent adopts is the method for " drug combination ", first used by base fertilizer, after enable biocontrol microorganisms field planting finally form dominant microflora in soil by repeatedly spraying, thus good preventive effect is played to black shank.
Summary of the invention
The object of the invention is the deficiency of preventing and treating black shank technology in order to solve the single drip irrigation of existing employing (liquid).Aim to provide a kind of bio-organic fertilizer containing bacillus cereus, this antibacterial fertilizer is combined the new technology of prevention and control disease with drip irrigation, the colonization ability of Antagonistic Fungi in soil can be improved by combined action, effectively improve it to the resistance of this disease.To improving vega microenvironment, promote that the development of biological prevention is significant.
The present invention is achieved through the following technical solutions:
A kind of bio-organic fertilizer containing bacillus cereus, by percentage to the quality, comprise bacillus cereus bacterium liquid 1% ~ 5%, scarp 35% ~ 40%, peat 35% ~ 45%, oil cake 8% ~ 10%, flower slag 9% ~ 12%, be preferably bacillus cereus bacterium liquid 1%, scarp 40%, peat 40%, oil cake 10%, flower slag 9%.
Described bacillus cereus adopts liquid nutrient medium to carry out fermentation culture, and this medium component is: glucose 2.0g/L ~ 2.8g/L, malt extract 2.0g/L ~ 2.5g/L, peptone 1.0g/L ~ 1.2g/L, NaCl1.0mg/L ~ 1.5mg/L, (NH4) 2sO 41.0mg/L ~ 1.5mg/L, anhydrous CaCl 20.1mg/L ~ 0.5mg/L, ZnSO 47H 2o0.1mg/L ~ 0.5mg/L, FeCl 36H 2o0.01mg/L ~ 0.05mg/L, pH6.5 ~ 7.2.Preferably, medium component is: glucose 2.0g/L, malt extract 2.0g/L, peptone 1.0g/L, NaCl1.2mg/L, (NH4) 2sO 41.5mg/L, anhydrous CaCl 20.2mg/L, ZnSO 47H 2o0.3mg/L, FeCl 36H 2o0.02mg/L, pH6.8, H 2o1000m1.
Described liquid nutrient medium is prepared by the following method: take said components respectively and be dissolved in 1L distilled water, rear pH meter to be dissolved regulates pH to 6.8, above-mentioned solution is loaded in triangular flask and is placed in Autoclave in 121 DEG C of sterilizings 20 minutes, after substratum cooling, pick 1ml bacillus cereus with transfering loop is transferred in this substratum, in 37 DEG C of shaking table 100rpm shaking culture 48hr.
Described glucose can also be sucrose;
Described peptone can also be that corn steep liquor or Tryptones substitute.
Described bacillus cereus is bacterium colony projection on LB solid medium, and the smooth of the edge is fine and smooth, is creamy white, diameter 1.1mm ~ 1.8mm.
Described microorganism organic fertilizer is prepared by the following method: by bacillus cereus bacterium liquid respectively with peat, oil cake, flower slag and carbon ammoniacal liquor Homogeneous phase mixing, the laggard windrow of water adding cumulative volume 10% is sealed, carried out a turning every 5 days, ferment after 40 days, this bio-organic fertilizer obtained.
Described microorganism organic fertilizer suppresses the purposes of black shank for combining, its using method is: first used as base fertilizer by this biological organic fertilizer, and 80g ~ 100g is used on every pool, uses 90kg ~ 110kg for every mu.Simultaneously by Bacillus cereus fermentation bacterium liquid according to 4000 ~ 5500 times of dilutions, pouring cigarette strain root after transplanting, every mu of consumption is 9kg ~ 12kg.At cigarette strain growth Seedling Stage, adopt pouring time more sparse, treat cigarette strain Later growth, when tobacco leaf is dense, adopt sprinkling irrigation.Use sprinkler irrigation system to spray on cigarette strain blade face, leaf portion and root spray at every turn and all will soak into, and within every 15 days, spray 1 time, spray 2 every month.
Beneficial effect of the present invention: microorganism organic fertilizer of the present invention has positive meaning for the growth promoting function of tobacco, effectively can prevent black shank in conjunction with the bacterium liquid of this biological bacteria is co-administered simultaneously, reach and do not use chemical pesticide and generation or the harm reduction degree that effectively can suppress black shank, the technology meets current agricultural and produces the requirement changed to green, environmental protection and Sustainable development, and to improve tobacco and tobacco product security, reduce smoking the harm aspect that health is brought also had great importance.
Embodiment
Below in conjunction with embodiment, the present invention is described further, the following stated, only to preferred embodiment of the present invention, not do other forms of restriction to the present invention, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equal change.Everyly do not depart from the present invention program's content, according to technical spirit of the present invention to any simple modification made for any of the above embodiments, equivalent variations and remodeling, all drop in protection scope of the present invention.
Embodiment 1
Containing a bio-organic fertilizer for bacillus cereus, be made up of bacillus cereus bacterium liquid 1kg, scarp 35kg, peat 45kg, oil cake 8kg, flower slag 9kg.
This bio-organic fertilizer is prepared by the following method:
1) glucose 2.0g/L, malt extract 2.0g/L, peptone 1.2g/L, NaCl1.0mg/L, (NH4) is taken respectively 2sO 41.5mg/L, anhydrous CaCl 20.3mg/L, ZnSO 47H 2o0.5mg/L, FeCl 36H 2o0.01mg/L, pH6.9;
2) be dissolved in 1L distilled water by said components, rear pH meter to be dissolved regulates pH to 6.8;
3) above-mentioned solution is loaded in triangular flask is placed in 121 DEG C of sterilizings 20 minutes in Autoclave, after substratum cooling, pick 1ml bacillus cereus with transfering loop be transferred in this substratum, in 37 DEG C of shaking table 100rpm shaking culture 48hr.
4) by the bacillus cereus bacterium liquid of preparation respectively with peat, oil cake, flower slag and carbon ammoniacal liquor Homogeneous phase mixing, the laggard windrow of water adding cumulative volume 10% is sealed, a turning is carried out every 5 days, ferment after 40 days, this bio-organic fertilizer obtained, this bacterial manure effective active bacterium (bacillus cereus) >=0.5 hundred million/g (cfu).
Embodiment 2
Containing a bio-organic fertilizer for bacillus cereus, be made up of bacillus cereus bacterium liquid 4kg, scarp 39kg, peat 35kg, oil cake 10kg, flower slag 12kg.
This bio-organic fertilizer is prepared by the following method:
1) glucose 2.3g/L, malt extract 2.4g/L, peptone 1.0g/L, NaCl1.3mg/L, (NH4) is taken respectively 2sO 41.5mg/L, anhydrous CaCl 20.2mg/L, ZnSO 47H 2o0.1mg/L, FeCl 36H 2o0.03mg/L, pH7.0, H 2o1000m1;
2) be dissolved in 1L distilled water by said components, rear pH meter to be dissolved regulates pH to 6.8;
3) above-mentioned solution is loaded in triangular flask is placed in 121 DEG C of sterilizings 20 minutes in Autoclave, after substratum cooling, pick 1ml bacillus cereus with transfering loop be transferred in this substratum, in 37 DEG C of shaking table 100rpm shaking culture 48hr.
4) by the bacillus cereus bacterium liquid of preparation respectively with peat, oil cake, flower slag and carbon ammoniacal liquor Homogeneous phase mixing, the laggard windrow of water adding cumulative volume 10% is sealed, a turning is carried out every 5 days, ferment after 40 days, this bio-organic fertilizer obtained, this bacterial manure effective active bacterium (bacillus cereus) >=0.5 hundred million/g (cfu).
Embodiment 3
Containing a bio-organic fertilizer for bacillus cereus, be made up of bacillus cereus bacterium liquid 1kg, scarp 40kg, peat 40kg, oil cake 10kg, flower slag 9kg.
This bio-organic fertilizer is prepared by the following method:
1) glucose 2.0g/L, malt extract 2.0g/L, peptone 1.0g/L, NaCl1.2mg/L, (NH4) is taken respectively 2sO 41.5mg/L, anhydrous CaCl 20.2mg/L, ZnSO 47H 2o0.3mg/L, FeCl 36H 2o0.02mg/L, pH6.8, H 2o1000m1;
2) be dissolved in 1L distilled water by said components, rear pH meter to be dissolved regulates pH to 6.8;
3) above-mentioned solution is loaded in triangular flask is placed in 121 DEG C of sterilizings 20 minutes in Autoclave, after substratum cooling, pick 1ml bacillus cereus with transfering loop be transferred in this substratum, in 37 DEG C of shaking table 100rpm shaking culture 48hr.
4) by the bacillus cereus bacterium liquid of preparation respectively with peat, oil cake, flower slag and carbon ammoniacal liquor Homogeneous phase mixing, the laggard windrow of water adding cumulative volume 10% is sealed, a turning is carried out every 5 days, ferment after 40 days, this bio-organic fertilizer obtained, this bacterial manure effective active bacterium (bacillus cereus) >=0.5 hundred million/g (cfu).
Embodiment 4 microbial organic fertilizer carries out detecting and identifying
Prepare bio-fertilizer according to embodiment 3 formula, under equal conditions detect containing bacillus cereus bio-fertilizer in traditional fertilizer, contrast content and the activity of two kinds of each components of fertilizer.
Table 1 detects data containing bacillus cereus bio-fertilizer and traditional fertilizer
Can be learnt by table 1, nitrogen content containing bacillus cereus bio-fertilizer of the present invention is higher than traditional fertilizer, always fat up and be also more or less the same, within the category of plant absorption, other trace ingredientss also meet the requirement of fertilising completely, but because bio-fertilizer is more easily received by soil in implementation process, its fertilizer efficiency is better than organic fertilizer.
Embodiment 5 is used for the effect of tobacco containing bacillus cereus bio-fertilizer
This experimental establishment is in inhabitants of Yuxi City in Yunnan Province, and bioorganism fertilizer is researched and developed by applicant, provided, and contrast fertilizer is the compoiste fertilizer of market routine.
Fertilising money is investigated the soil balck shank state of an illness, selects two pieces of state of an illness experimental field suitable, uses bio-fertilizer and compoiste fertilizer according to certain metering, applies fertilizer and investigates disease index respectively and calculate preventive effect for latter 30 days, 100 days.Experimental result is as follows:
Table 2 is used for the preventive effect of black shank containing bacillus cereus bio-fertilizer
Learnt by table 2, bio-feritlizer can effectively preventing black shank, the highest can reach 98.46% preventive effect, contrast tool with compoiste fertilizer to have an enormous advantage, secondly, bio-feritlizer has very large promoter action for the raising of output and products thereof quality, facilitates the income of peasant greatly, has market economy benefit.

Claims (6)

1. containing a microorganism organic fertilizer for bacillus cereus, it is characterized in that: by percentage to the quality, comprise bacillus cereus bacterium liquid 1% ~ 5%, scarp 35% ~ 40%, peat 35% ~ 45%, oil cake 8% ~ 10%, flower slag 9% ~ 12%.
2. a kind of microorganism organic fertilizer containing Bacillus cereus according to claim 1, is characterized in that: by percentage to the quality, comprises bacillus cereus bacterium liquid 1%, scarp 40%, peat 40%, oil cake 10%, flower slag 9%.
3. a kind of microorganism organic fertilizer containing Bacillus cereus according to claim 1, it is characterized in that: described bacillus cereus adopts liquid nutrient medium to carry out fermentation culture, and this medium component is as follows: glucose 2.0g/L ~ 2.8g/L, malt extract 2.0g/L ~ 2.5g/L, peptone 1.0g/L ~ 1.2g/L, NaCl1.0mg/L ~ 1.5mg/L, (NH4) 2sO 41.0mg/L ~ 1.5mg/L, anhydrous CaCl 20.1mg/L ~ 0.5mg/L, ZnSO 47H 2o0.1mg/L ~ 0.5mg/L, FeCl 36H 2o0.01mg/L ~ 0.05mg/L, pH6.5 ~ 7.2.
4. a kind of microorganism organic fertilizer containing Bacillus cereus according to claim 3, is characterized in that: described substratum comprises glucose 2.0g/L, malt extract 2.0g/L, peptone 1.0g/L, NaCl1.2mg/L, (NH4) 2sO 41.5mg/L, anhydrous CaCl 20.2mg/L, ZnSO 47H 2o0.3mg/L, FeCl 36H 2o0.02mg/L, pH6.8, H 2o1000m1.
5. a kind of microorganism organic fertilizer containing Bacillus cereus according to claim 1, it is characterized in that: this microbial organic fertilizer is prepared by the following method: by bacillus cereus bacterium liquid respectively with peat, oil cake, flower slag and carbon ammoniacal liquor Homogeneous phase mixing, the laggard windrow of water adding cumulative volume 10% is sealed, a turning is carried out every 5 days, ferment after 40 days, this bio-organic fertilizer obtained.
6. a kind of microorganism organic fertilizer containing Bacillus cereus according to claim 1 and 2, is characterized in that: described microorganism organic fertilizer suppresses the purposes of black shank for combining.
CN201410460497.5A 2014-09-11 2014-09-11 A kind of microbial organic fertilizer containing bacillus cereus Active CN104230563B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410460497.5A CN104230563B (en) 2014-09-11 2014-09-11 A kind of microbial organic fertilizer containing bacillus cereus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410460497.5A CN104230563B (en) 2014-09-11 2014-09-11 A kind of microbial organic fertilizer containing bacillus cereus

Publications (2)

Publication Number Publication Date
CN104230563A true CN104230563A (en) 2014-12-24
CN104230563B CN104230563B (en) 2018-03-06

Family

ID=52219580

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410460497.5A Active CN104230563B (en) 2014-09-11 2014-09-11 A kind of microbial organic fertilizer containing bacillus cereus

Country Status (1)

Country Link
CN (1) CN104230563B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109761656A (en) * 2019-03-11 2019-05-17 云南省农业科学院生物技术与种质资源研究所 A kind of microbial-bacterial fertilizer combines the method for its fermentation liquid raising potassium content of tobacco leaf
CN109836277A (en) * 2019-03-11 2019-06-04 云南省农业科学院生物技术与种质资源研究所 A kind of microbial-bacterial fertilizer combines the method for fermented liquid inhibition tobacco black shank

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103087944A (en) * 2012-12-07 2013-05-08 贵州省烟草公司毕节市公司 Bacilluscereus and application thereof
CN103708855A (en) * 2013-12-27 2014-04-09 云南中电新能源有限公司 Microbial fertilizer or pesticide preparation method and prepared microbial fertilizer or pesticide

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103087944A (en) * 2012-12-07 2013-05-08 贵州省烟草公司毕节市公司 Bacilluscereus and application thereof
CN103708855A (en) * 2013-12-27 2014-04-09 云南中电新能源有限公司 Microbial fertilizer or pesticide preparation method and prepared microbial fertilizer or pesticide

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
丛铭 等: "烟草疫霉拮抗菌筛选、鉴定及发酵条件研究", 《中国酿造》 *
曹明慧: "烟草黑胫病拮抗菌的筛选及其生物效应", 《土壤学报》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109761656A (en) * 2019-03-11 2019-05-17 云南省农业科学院生物技术与种质资源研究所 A kind of microbial-bacterial fertilizer combines the method for its fermentation liquid raising potassium content of tobacco leaf
CN109836277A (en) * 2019-03-11 2019-06-04 云南省农业科学院生物技术与种质资源研究所 A kind of microbial-bacterial fertilizer combines the method for fermented liquid inhibition tobacco black shank

Also Published As

Publication number Publication date
CN104230563B (en) 2018-03-06

Similar Documents

Publication Publication Date Title
CN104928201B (en) A kind of bacillus amyloliquefaciens HN-11 and its microbial inoculum
CN101555459B (en) Strain SB177 for controlling strawberry replant diseases and preparation thereof
CN101781569B (en) Soil improvement and anti continuous cropping multifunctional biological agent
WO2011032330A1 (en) Antagonistic bacteria for preventing and treating bacterial wilt disease of continuously planted tobacco and microorganism organic fertilizer thereof
CN103464453B (en) A kind of method of repairing soil-borne disease soil
CN107502572A (en) Application of the bacillus licheniformis in straw degradative, the microbial bacterial agent comprising the bacterium and its application
CN103651582B (en) Broad-spectrum composite microbial fungicide and preparation method thereof
CN102504825B (en) Ecologic conditioner for salt-affected soil by sea and preparation method and application of ecologic conditioner
CN104692957B (en) Biological organic fertilizer and its application containing bacillus subtilis
CN102120969B (en) Biocontrol microorganism of wide spectrum antagonistic plant pathogenic fungi and application thereof
CN103013838A (en) Pasty biocontrol preparation for strawberry greensickness, and preparation method, application and special strain thereof
CN102899266B (en) Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 and application
CN103749229B (en) Cultivation mode for combining paddy rice-banana crop rotation with banana fusarium wilt prevention and control by means of adding straw
CN103667134A (en) Paenibacillus polymyxa N3-4 used for preventing and treating clubroot of cruciferous crops and application thereof
CN109136123A (en) One plant of nematode biocontrol microorganisms and its application
CN103270915A (en) Method for preventing and treating tobacco bacterial wilt
CN102515977A (en) Water-holding root-promoting halophilic alkaliphilic microorganism root dipping liquid and preparation and application thereof
CN101781565B (en) Multifunctional biological agent containing Trichoderma pseudokiningii
CN103141519A (en) Preparation method and application of anti-biological inoculants for controlling crop soil-borne diseases
CN103951483B (en) A kind of prevent and treat farm crop soil-borne disease biocontrol bacterial fertilizer and production technique and application
CN106673736A (en) Microbial decomposing agent and humus prepared thereby
CN106399160B (en) A kind of acid soil vigor remediation microbial inoculum and preparation method thereof
CN104230563A (en) Microbial organic fertilizer containing Bacillus cereus
CN107324950A (en) A kind of technology for the complex micro organism fungicide that tobacco planting is used
CN105624062A (en) Ageratina adenophora organic fertilizer solid microbial inoculant and preparation method thereof, and application of microbial inoculant in organic fertilizer production

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
CB03 Change of inventor or designer information

Inventor after: Zhu Haibin

Inventor after: Tao Nan

Inventor after: Wang Yaojin

Inventor after: Dai Bi

Inventor after: Xu Jie

Inventor after: Zhou Xiaogang

Inventor after: Wang Shaokun

Inventor after: Yang Yingming

Inventor after: Wang Chao

Inventor after: Luo Huayuan

Inventor after: Yao Chunxin

Inventor after: Ding Yumei

Inventor before: Xu Jie

Inventor before: Ding Yumei

Inventor before: Tao Nan

Inventor before: Dai Bi

Inventor before: Wang Shaokun

Inventor before: Zhou Xiaogang

Inventor before: Wang Yaojin

Inventor before: Wang Chao

Inventor before: Yang Yingming

Inventor before: Luo Huayuan

Inventor before: Zhu Haibin

Inventor before: Yao Chunxin

GR01 Patent grant
GR01 Patent grant