CN104230563B - A kind of microbial organic fertilizer containing bacillus cereus - Google Patents
A kind of microbial organic fertilizer containing bacillus cereus Download PDFInfo
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- CN104230563B CN104230563B CN201410460497.5A CN201410460497A CN104230563B CN 104230563 B CN104230563 B CN 104230563B CN 201410460497 A CN201410460497 A CN 201410460497A CN 104230563 B CN104230563 B CN 104230563B
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- bacillus cereus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
Present invention relates particularly to a kind of microbial organic fertilizer containing Bacillus cereus, include bacillus cereus bacterium solution 1%~5%, scarp 35%~40%, peat 35%~45%, oil cake 8%~10%, flower slag 9%~12%, by above raw material by evenly mixing after, add the water of cumulative volume 10%, stacking fermentation.A turning was carried out every 5 days, after fermenting 40 days, the bio-organic fertilizer, the bacterial manure effective active bacterium (bacillus cereus) >=0.5 hundred million/g (cfu) is made.Applied the biological organic fertilizer as base fertilizer, while Bacillus cereus fermentation bacterium solution poured after transplanting and cigarette strain root or sprayed in the page according to 5000 times of dilutions, can effective prevention and control tobacco black shank, environmental pollution caused by reduction chemical agent use.
Description
Technical field
The invention belongs to biological pesticide technical field, is related to a kind of bio-organic fertilizer containing bacillus cereus, specifically
Say to be exactly that the microbial-bacterial fertilizer of bacillus cereus production is combined into the fermented liquid mutual association to suppress tobacco black shank in ground
Occur.
Background technology
Tobacco black shank (Phytophthora parasitica var.nicotiane) is the destructive disease of tobacco,
One of disease of most serious is endangered simultaneously and in World tobacco production, the disease, which is respectively produced cigarette in China and saved, different degrees of hair
It is raw, and endanger more universal and serious.In recent years because continuous cropping vega area expands and the growth of the continuous cropping time limit, many places
Tobacco black shank, which has, aggravates popular trend.People lose caused by order to mitigate the disease for many years, once try out cultural control, change
A variety of method control tobacco black shanks such as preventing and treating, breeding for disease resistance are learned, but because the zoospore of the germ forms speed soon and counts
Amount is big, infects short incubation period, rapid onset after cigarette strain, can occur repeatedly to infect again in a season of growth, while the disease is sometimes
Generation also is mixed with other diseases, so as to cause the forfeiture of some anti-balck shank varietal resistances；Moreover, the large area of disease-resistant variety
Single cropping in flakes year after year, layout is unreasonable, easily loses the resistance of improved seeds, shortens the service life of disease-resistant variety, and
And it is difficult to control if plant disease epidemic.At present, the chemical agent for being used for preventing and treating tobacco black shank in production is mostly phenyl amide
Class and aliette etc. be same or several systemic fungicides of mechanism of action identical, it is easy to drug resistance is produced, so as to cause
Drug effect declines, dosing increase.Tobacco be leaf with industrial crops, outward appearance and inherent quality to tobacco leaf have special requirement,
Residues of pesticides, pathogenic bacteria resistance to drugs and the influence to cigarette health greatly limit the extensive utilization of agricultural chemicals.In tobacco and
Under the trend that tobacco product gradually develops to nuisanceless direction, biological control is as a kind of safe and effective method and increasingly
It is taken seriously.
The antagonistic bacterium of tobacco black shank bacterium has Pseudomonas alba (Pseudomonas spp.) and bacillus (Ba-
Cillus spp.), it mainly makes tobacco black shank bacterium myceliolysis by producing antibiotic；By adding in field soil
Enter antagonistic bacterium and find that can significantly reduce tobacco black shank bacterium infects chance, so as to reduce the incidence of disease.What is used at present is main
Exactly concentrated liquid preparation, pure spore pulvis or complex solid pulvis is made in Antagonistic Fungi by method, then before tobacco seedlings transplanting
Using.These methods are more single, because biocontrol bacterial strain is influenceed by complicated soil ecological factor, biocontrol agent preventive effect it is steady
It is qualitative to cannot be guaranteed, preventive effect unobvious.And used by this item patent it is the method for " drug combination ", first pass through base fertilizer and apply
With, after biocontrol microorganisms is colonized in soil by repeatedly spraying and ultimately form dominant microflora, so as to the black shin of tobacco
Disease plays preferable preventive effect.
The content of the invention
The invention aims to solve the single drip irrigation of existing use (liquid) preventing and treating tobacco black shank technology not
Foot.A kind of bio-organic fertilizer containing bacillus cereus is aimed to provide, antibacterial fertilizer is combined into prevention and control disease with drip irrigation
New technology, colonization ability of the Antagonistic Fungi in soil can be improved by synergy, it is effectively improved and disease is resisted
Property.To improving vega microenvironment, promote the development of biological prevention significant.
The present invention is achieved through the following technical solutions：
A kind of bio-organic fertilizer containing bacillus cereus, by percentage to the quality, include bacillus cereus bacterium solution
1%~5%, scarp 35%~40%, peat 35%~45%, oil cake 8%~10%, flower slag 9%~12%, it is preferably waxy
Bacillus bacterium solution 1%, scarp 40%, peat 40%, oil cake 10%, flower slag 9%.
Described bacillus cereus carries out fermented and cultured using fluid nutrient medium, and the medium component is：Glucose
2.0g/L~2.8g/L, malt extract 2.0g/L~2.5g/L, peptone 1.0g/L~1.2g/L, NaCl1.0mg/L~
1.5mg/L、(NH4)2SO41.0mg/L~1.5mg/L, anhydrous CaCl20.1mg/L~0.5mg/L, ZnSO4·7H2O0.1mg/L
~0.5mg/L, FeCl3·6H2O0.01mg/L~0.05mg/L, pH6.5~7.2.Preferably, medium component is：Glucose
2.0g/L, malt extract 2.0g/L, peptone 1.0g/L, NaCl1.2mg/L, (NH4)2SO4It is 1.5mg/L, anhydrous
CaCl20.2mg/L、ZnSO4·7H2O0.3mg/L、FeCl3·6H2O0.02mg/L、pH6.8、H2O1000m1。
Described fluid nutrient medium is prepared by the following method：Said components are weighed respectively and are dissolved in 1L distilled water, are treated
After dissolving with pH meter adjust pH to 6.8, by above-mentioned solution be loaded on triangular flask in be placed in autoclave in 121 DEG C sterilize 20 minutes,
1ml bacillus cereus is picked with oese to be transferred in the culture medium, shaken in 37 DEG C of shaking table 100rpm after culture medium cooling
Swing culture 48hr.
Described glucose can also be sucrose；
Described peptone can also be that corn steep liquor or tryptone substitute.
Described bacillus cereus bacterium colony projection on LB solid mediums, the smooth of the edge is fine and smooth, is creamy white, diameter
1.1mm~1.8mm.
Described microbial organic fertilizer is prepared by the following method：By bacillus cereus bacterium solution respectively with peat, oil
Withered, flower slag and carbon ammoniacal liquor are uniformly mixed, and stacking is carried out after adding the water of cumulative volume 10%, and a turning, hair were carried out every 5 days
After ferment 40 days, the bio-organic fertilizer is made.
Described microbial organic fertilizer is used to combine the purposes for suppressing tobacco black shank, and its application method is：First will
The biological organic fertilizer is applied as base fertilizer, and 80g~100g is applied per the pool, and every mu is applied 90kg~110kg.Simultaneously by waxy gemma
Bacillus fermentation bacterium solution pours cigarette strain root, every mu of dosage is 9kg~12kg according to 4000~5500 times of dilutions after transplanting.In cigarette
Strain growth Seedling Stage, than it is sparse when using pour, treat cigarette strain Later growth, when tobacco leaf is dense using sprinkling irrigation.It is using sprinkling irrigation
System is sprayed on cigarette strain blade face, and leaf portion and root spray be intended to be impregnated with every time, spray 1 time within every 15 days, spray every month 2 times.
Beneficial effects of the present invention：Microbial organic fertilizer of the present invention has positive meaning for the growth promoting function of tobacco
Justice, the bacterium solution combined administration in combination with the biological bacteria can effectively prevent tobacco black shank, reach and do not apply chemical pesticide and energy
Effectively suppress generation or the harm reduction degree of tobacco black shank, the technology meets current agricultural and produced to green, environmental protection
The requirement changed with sustainable development, and to improving the security of tobacco and tobacco product, reducing what smoking came to health care belt
Also have great importance in terms of harm.
Embodiment
With reference to embodiment, the present invention is described further, as described below, is only the preferable implementation to the present invention
Example, is not limited the present invention, any person skilled in the art is possibly also with the disclosure above
Technology contents changed or be modified as the equivalent embodiment changed on an equal basis.It is every without departing from the present invention program content, foundation
The technical spirit of the present invention all falls within the present invention's to any simple modification, equivalent variations and remodeling made for any of the above embodiments
In protection domain.
Embodiment 1
A kind of bio-organic fertilizer containing bacillus cereus, by bacillus cereus bacterium solution 1kg, scarp 35kg, peat
45kg, oil cake 8kg, flower slag 9kg compositions.
The bio-organic fertilizer is prepared by the following method：
1) weigh respectively glucose 2.0g/L, malt extract 2.0g/L, peptone 1.2g/L, NaCl1.0mg/L,
(NH4)2SO41.5mg/L, anhydrous CaCl20.3mg/L、ZnSO4·7H2O0.5mg/L、FeCl3·6H2O0.01mg/L、pH6.9；
2) by said components and it is dissolved in 1L distilled water, with pH meter pH to 6.8 is adjusted after to be dissolved；
3) above-mentioned solution is loaded in triangular flask to be placed in autoclave and sterilized 20 minutes in 121 DEG C, after culture medium cooling
1ml bacillus cereus is picked with oese to be transferred in the culture medium, in 37 DEG C of shaking table 100rpm shaken cultivations 48hr.
4) the bacillus cereus bacterium solution of preparation is uniformly mixed with peat, oil cake, flower slag and carbon ammoniacal liquor respectively, added
Stacking is carried out after the water of cumulative volume 10%, a turning was carried out every 5 days, after fermenting 40 days, the bio-organic fertilizer is made, should
Bacterial manure effective active bacterium (bacillus cereus) >=0.5 hundred million/g (cfu).
Embodiment 2
A kind of bio-organic fertilizer containing bacillus cereus, by bacillus cereus bacterium solution 4kg, scarp 39kg, peat
35kg, oil cake 10kg, flower slag 12kg compositions.
The bio-organic fertilizer is prepared by the following method：
1) weigh respectively glucose 2.3g/L, malt extract 2.4g/L, peptone 1.0g/L, NaCl1.3mg/L,
(NH4)2SO41.5mg/L, anhydrous CaCl20.2mg/L、ZnSO4·7H2O0.1mg/L、FeCl3·6H2O0.03mg/L、pH7.0、
H2O1000m1；
2) by said components and it is dissolved in 1L distilled water, with pH meter pH to 6.8 is adjusted after to be dissolved；
3) above-mentioned solution is loaded in triangular flask to be placed in autoclave and sterilized 20 minutes in 121 DEG C, after culture medium cooling
1ml bacillus cereus is picked with oese to be transferred in the culture medium, in 37 DEG C of shaking table 100rpm shaken cultivations 48hr.
4) the bacillus cereus bacterium solution of preparation is uniformly mixed with peat, oil cake, flower slag and carbon ammoniacal liquor respectively, added
Stacking is carried out after the water of cumulative volume 10%, a turning was carried out every 5 days, after fermenting 40 days, the bio-organic fertilizer is made, should
Bacterial manure effective active bacterium (bacillus cereus) >=0.5 hundred million/g (cfu).
Embodiment 3
A kind of bio-organic fertilizer containing bacillus cereus, by bacillus cereus bacterium solution 1kg, scarp 40kg, peat
40kg, oil cake 10kg, flower slag 9kg compositions.
The bio-organic fertilizer is prepared by the following method：
1) weigh respectively glucose 2.0g/L, malt extract 2.0g/L, peptone 1.0g/L, NaCl1.2mg/L,
(NH4)2SO41.5mg/L, anhydrous CaCl20.2mg/L、ZnSO4·7H2O0.3mg/L、FeCl3·6H2O0.02mg/L、pH6.8、
H2O1000m1；
2) by said components and it is dissolved in 1L distilled water, with pH meter pH to 6.8 is adjusted after to be dissolved；
3) above-mentioned solution is loaded in triangular flask to be placed in autoclave and sterilized 20 minutes in 121 DEG C, after culture medium cooling
1ml bacillus cereus is picked with oese to be transferred in the culture medium, in 37 DEG C of shaking table 100rpm shaken cultivations 48hr.
4) the bacillus cereus bacterium solution of preparation is uniformly mixed with peat, oil cake, flower slag and carbon ammoniacal liquor respectively, added
Stacking is carried out after the water of cumulative volume 10%, a turning was carried out every 5 days, after fermenting 40 days, the bio-organic fertilizer is made, should
Bacterial manure effective active bacterium (bacillus cereus) >=0.5 hundred million/g (cfu).
The microbial organic fertilizer of embodiment 4 is detected and identified
Bio-fertilizer is prepared according to the formula of embodiment 3, will under equal conditions contain bacillus cereus bio-fertilizer in traditional
Organic fertilizer detected, contrast two kinds of fertilizer each components content and activity.
The bio-fertilizer containing bacillus cereus of table 1 and traditional organic fertilizer detection data
It is total to support by table 1 it is known that the nitrogen content of the bio-fertilizer containing bacillus cereus of the present invention is higher than traditional organic fertilizer
Fertilizer is also more or less the same, and within the category of plant absorption, other micro constitutents are also fully consistent with the requirement of fertilising, but due to biology
Fertilizer is easier to be received by soil in implementation process, and its fertilizer efficiency is better than organic fertilizer.
The bio-fertilizer containing bacillus cereus of embodiment 5 is used for the effect of tobacco
This experimental arrangement is researched and developed by applicant, provided in inhabitants of Yuxi City in Yunnan Province, bioorganism fertilizer, and control fertilizer is that market is normal
The compoiste fertilizer of rule.
Fertilising money is investigated the soil balck shank state of an illness, two pieces of state of an illness is selected quite experimental field, according to certain metering
Using bio-fertilizer and compoiste fertilizer, 30 days after fertilising, disease index is investigated respectively within 100 days and calculate preventive effect.Experimental result is such as
Shown in lower：
The bio-fertilizer containing bacillus cereus of table 2 is used for the preventive effect of tobacco black shank
To be learnt by table 2, bio-feritlizer can reach 98.46% preventive effect with effectively preventing tobacco black shank, highest,
Had an enormous advantage with compoiste fertilizer contrast tool, secondly, raising of the bio-feritlizer for yield and products thereof quality has
Very big facilitation, has greatly facilitated the income of peasant, has market economy benefit.
Claims (3)
- A kind of 1. microbial organic fertilizer containing bacillus cereus, it is characterised in that：By percentage to the quality, comprising waxy bud Spore bacillus bacterium solution 1%~5%, scarp 35%~40%, peat 35%~45%, oil cake 8%~10%, flower slag 9%~12%；Described bacillus cereus carries out fermented and cultured using fluid nutrient medium, and the medium component is as follows：Glucose 2.0g/ L, malt extract 2.0g/L, peptone 1.0g/L, NaCl1.2mg/L, (NH4)2SO41.5mg/L, anhydrous CaCl2 0.2mg/ L、ZnSO4·7H2O 0.3mg/L、FeCl3·6H2O 0.02mg/L、H2O 1000mL；The microbial organic fertilizer is prepared by the following method：By bacillus cereus bacterium solution respectively with peat, oil cake, flower slag and Carbon ammoniacal liquor is uniformly mixed, and stacking is carried out after adding the water of cumulative volume 10%, and a turning was carried out every 5 days, after fermenting 40 days, system Obtain the bio-organic fertilizer.
- A kind of 2. microbial organic fertilizer containing bacillus cereus according to claim 1, it is characterised in that：With quality Percentages, include bacillus cereus bacterium solution 1%, scarp 40%, peat 40%, oil cake 10%, flower slag 9%.
- A kind of 3. microbial organic fertilizer containing bacillus cereus according to claim 1 or 2, it is characterised in that：Institute The microbial organic fertilizer stated is used to combine the purposes for suppressing tobacco black shank.
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| CN109761656A (en) * | 2019-03-11 | 2019-05-17 | 云南省农业科学院生物技术与种质资源研究所 | A kind of microbial-bacterial fertilizer combines the method for its fermentation liquid raising potassium content of tobacco leaf |
| CN109836277A (en) * | 2019-03-11 | 2019-06-04 | 云南省农业科学院生物技术与种质资源研究所 | A kind of microbial-bacterial fertilizer combines the method for fermented liquid inhibition tobacco black shank |
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| CN103708855B (en) * | 2013-12-27 | 2016-03-23 | 云南中电新能源有限公司 | The preparation method of microbial fertilizer or agricultural chemicals and obtained microbial fertilizer or agricultural chemicals |
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Inventor after: Zhu Haibin Inventor after: Tao Nan Inventor after: Wang Yaojin Inventor after: Dai Bi Inventor after: Xu Jie Inventor after: Zhou Xiaogang Inventor after: Wang Shaokun Inventor after: Yang Yingming Inventor after: Wang Chao Inventor after: Luo Huayuan Inventor after: Yao Chunxin Inventor after: Ding Yumei Inventor before: Xu Jie Inventor before: Ding Yumei Inventor before: Tao Nan Inventor before: Dai Bi Inventor before: Wang Shaokun Inventor before: Zhou Xiaogang Inventor before: Wang Yaojin Inventor before: Wang Chao Inventor before: Yang Yingming Inventor before: Luo Huayuan Inventor before: Zhu Haibin Inventor before: Yao Chunxin |
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