CN109810968B - 胰酶的制备方法 - Google Patents
胰酶的制备方法 Download PDFInfo
- Publication number
- CN109810968B CN109810968B CN201910189916.9A CN201910189916A CN109810968B CN 109810968 B CN109810968 B CN 109810968B CN 201910189916 A CN201910189916 A CN 201910189916A CN 109810968 B CN109810968 B CN 109810968B
- Authority
- CN
- China
- Prior art keywords
- pancreatin
- pancreas
- ethanol
- phosphate
- producing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 108010019160 Pancreatin Proteins 0.000 title claims abstract description 71
- 229940055695 pancreatin Drugs 0.000 title claims abstract description 70
- 238000000034 method Methods 0.000 title claims description 25
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 62
- 241000700605 Viruses Species 0.000 claims abstract description 34
- KFSLWBXXFJQRDL-UHFFFAOYSA-N Peracetic acid Chemical compound CC(=O)OO KFSLWBXXFJQRDL-UHFFFAOYSA-N 0.000 claims abstract description 31
- 210000000496 pancreas Anatomy 0.000 claims abstract description 27
- 238000001035 drying Methods 0.000 claims abstract description 18
- 230000001376 precipitating effect Effects 0.000 claims abstract description 10
- 159000000007 calcium salts Chemical class 0.000 claims abstract description 9
- 229910019142 PO4 Inorganic materials 0.000 claims abstract description 8
- 230000000415 inactivating effect Effects 0.000 claims abstract description 8
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims abstract description 8
- 239000010452 phosphate Substances 0.000 claims abstract description 8
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 claims abstract description 8
- 238000002360 preparation method Methods 0.000 claims abstract description 6
- 238000002791 soaking Methods 0.000 claims abstract description 6
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 4
- 239000012535 impurity Substances 0.000 claims abstract description 4
- 239000002994 raw material Substances 0.000 claims description 23
- 239000002244 precipitate Substances 0.000 claims description 18
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 14
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 14
- 238000005238 degreasing Methods 0.000 claims description 14
- 239000000243 solution Substances 0.000 claims description 13
- 238000000227 grinding Methods 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 9
- 230000008014 freezing Effects 0.000 claims description 8
- 238000007710 freezing Methods 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 8
- VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical group [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 claims description 7
- 239000001639 calcium acetate Substances 0.000 claims description 7
- 235000011092 calcium acetate Nutrition 0.000 claims description 7
- 229960005147 calcium acetate Drugs 0.000 claims description 7
- 230000000937 inactivator Effects 0.000 claims description 7
- 230000003213 activating effect Effects 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 210000001198 duodenum Anatomy 0.000 claims description 4
- 239000000839 emulsion Substances 0.000 claims description 4
- 239000000725 suspension Substances 0.000 claims description 4
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims description 3
- 239000005711 Benzoic acid Substances 0.000 claims description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 2
- 239000005708 Sodium hypochlorite Substances 0.000 claims description 2
- ZRIUUUJAJJNDSS-UHFFFAOYSA-N ammonium phosphates Chemical compound [NH4+].[NH4+].[NH4+].[O-]P([O-])([O-])=O ZRIUUUJAJJNDSS-UHFFFAOYSA-N 0.000 claims description 2
- 235000010233 benzoic acid Nutrition 0.000 claims description 2
- 239000001110 calcium chloride Substances 0.000 claims description 2
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 2
- 150000004965 peroxy acids Chemical class 0.000 claims description 2
- 238000003825 pressing Methods 0.000 claims description 2
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 2
- MWNQXXOSWHCCOZ-UHFFFAOYSA-L sodium;oxido carbonate Chemical compound [Na+].[O-]OC([O-])=O MWNQXXOSWHCCOZ-UHFFFAOYSA-L 0.000 claims description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 2
- 239000007858 starting material Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 9
- 239000002158 endotoxin Substances 0.000 abstract description 8
- 230000002779 inactivation Effects 0.000 abstract description 8
- 239000003814 drug Substances 0.000 abstract description 6
- 238000000605 extraction Methods 0.000 abstract description 6
- 239000000047 product Substances 0.000 abstract description 6
- 239000001488 sodium phosphate Substances 0.000 abstract description 6
- 229910000406 trisodium phosphate Inorganic materials 0.000 abstract description 6
- 235000019801 trisodium phosphate Nutrition 0.000 abstract description 6
- 238000004140 cleaning Methods 0.000 abstract description 4
- 241001465754 Metazoa Species 0.000 abstract description 3
- 239000003960 organic solvent Substances 0.000 abstract description 2
- 239000012466 permeate Substances 0.000 abstract description 2
- 230000004913 activation Effects 0.000 abstract 1
- 239000012190 activator Substances 0.000 abstract 1
- 239000001506 calcium phosphate Substances 0.000 abstract 1
- 229910000389 calcium phosphate Inorganic materials 0.000 abstract 1
- 235000011010 calcium phosphates Nutrition 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 239000002002 slurry Substances 0.000 abstract 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 abstract 1
- 239000004382 Amylase Substances 0.000 description 4
- 102000013142 Amylases Human genes 0.000 description 4
- 108010065511 Amylases Proteins 0.000 description 4
- 102000019280 Pancreatic lipases Human genes 0.000 description 4
- 108050006759 Pancreatic lipases Proteins 0.000 description 4
- 108090000631 Trypsin Proteins 0.000 description 4
- 102000004142 Trypsin Human genes 0.000 description 4
- 235000019418 amylase Nutrition 0.000 description 4
- 229940116369 pancreatic lipase Drugs 0.000 description 4
- 238000001556 precipitation Methods 0.000 description 4
- 239000012588 trypsin Substances 0.000 description 4
- 239000000428 dust Substances 0.000 description 3
- 229940062190 pancreas extract Drugs 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 238000010257 thawing Methods 0.000 description 3
- 230000003612 virological effect Effects 0.000 description 3
- 208000035404 Autolysis Diseases 0.000 description 2
- 206010057248 Cell death Diseases 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 241000724675 Hepatitis E virus Species 0.000 description 2
- 208000035467 Pancreatic insufficiency Diseases 0.000 description 2
- 241000202347 Porcine circovirus Species 0.000 description 2
- 241000702619 Porcine parvovirus Species 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 230000000762 glandular Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000028043 self proteolysis Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 241000701386 African swine fever virus Species 0.000 description 1
- 102000015790 Asparaginase Human genes 0.000 description 1
- 108010024976 Asparaginase Proteins 0.000 description 1
- 102000005367 Carboxypeptidases Human genes 0.000 description 1
- 108010006303 Carboxypeptidases Proteins 0.000 description 1
- 108090000317 Chymotrypsin Proteins 0.000 description 1
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 108060005987 Kallikrein Proteins 0.000 description 1
- 102000001399 Kallikrein Human genes 0.000 description 1
- 108010067372 Pancreatic elastase Proteins 0.000 description 1
- 102000016387 Pancreatic elastase Human genes 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 239000008186 active pharmaceutical agent Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229960003272 asparaginase Drugs 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-M asparaginate Chemical compound [O-]C(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-M 0.000 description 1
- WXBLLCUINBKULX-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1.OC(=O)C1=CC=CC=C1 WXBLLCUINBKULX-UHFFFAOYSA-N 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 229960002376 chymotrypsin Drugs 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 206010061428 decreased appetite Diseases 0.000 description 1
- 239000003866 digestant Substances 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 229940126534 drug product Drugs 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229940039088 kininogenase Drugs 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 208000024691 pancreas disease Diseases 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 238000012502 risk assessment Methods 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 230000000472 traumatic effect Effects 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Description
Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910189916.9A CN109810968B (zh) | 2019-03-13 | 2019-03-13 | 胰酶的制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910189916.9A CN109810968B (zh) | 2019-03-13 | 2019-03-13 | 胰酶的制备方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN109810968A CN109810968A (zh) | 2019-05-28 |
CN109810968B true CN109810968B (zh) | 2021-03-02 |
Family
ID=66608876
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910189916.9A Active CN109810968B (zh) | 2019-03-13 | 2019-03-13 | 胰酶的制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN109810968B (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112708611A (zh) * | 2021-01-19 | 2021-04-27 | 四川菲德力制药有限公司 | 一种胰酶制备方法 |
CN117844795B (zh) * | 2024-01-29 | 2024-07-09 | 四川菲德力制药有限公司 | 一种从动物胰脏中提取胰酶的方法 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103215246B (zh) * | 2013-04-11 | 2014-09-10 | 重庆奥力生物制药有限公司 | 胰酶的新生产工艺 |
CN104031900A (zh) * | 2014-06-26 | 2014-09-10 | 上海第一生化药业有限公司 | 一种去除多肽中的内毒素的方法 |
CN113750224B (zh) * | 2015-05-19 | 2024-04-30 | 科学蛋白实验室有限责任公司 | 在生产胰酶过程中减少或灭活病毒和微生物内容物的方法 |
CN108795920B (zh) * | 2018-06-22 | 2022-04-08 | 苏州良辰生物医药科技有限公司 | 一种胰酶的制备方法 |
-
2019
- 2019-03-13 CN CN201910189916.9A patent/CN109810968B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
CN109810968A (zh) | 2019-05-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN109810968B (zh) | 胰酶的制备方法 | |
CN108866137A (zh) | 鱼皮/鱼鳞胶原蛋白肽的制备方法 | |
EP2125865A1 (en) | Solubilization of protease crystals in fermentation broth | |
EP0116232B1 (en) | Antitumor agent and process for manufacturing said agent | |
CN112941128B (zh) | 一种从银耳中提取银耳多糖的方法 | |
CN108795920B (zh) | 一种胰酶的制备方法 | |
JP2013116101A (ja) | 食物繊維含量の高い酵母細胞壁画分 | |
TW201809261A (zh) | 可食用真菌 | |
US9676817B2 (en) | Method for the removal of virus from a protein solution | |
CN111647584B (zh) | 低温酸性蛋白酶PsAPA及其制备方法和应用 | |
WO2019107628A1 (ko) | 파인애플 가공 부산물을 이용한 유기성 폐기물의 처리방법 | |
CN110894518A (zh) | 一种猴头菇小肽的制备方法 | |
CN108191991A (zh) | 一种杏鲍菇多糖的提纯方法 | |
CN102296101B (zh) | 一种微胶囊包埋的紫菜降血压肽的制备方法 | |
US20190127494A1 (en) | Method for Obtaining at Least One or More Beta-Glucan Compounds or a Solids Suspension Containing Beta Glucan from Yeast Cells | |
CN105274033A (zh) | 一种高效去除大型海藻表面附生微生物的方法 | |
JPH01247086A (ja) | エンドチア・パラシチカからプロテアーゼの製法 | |
CN110982813B (zh) | 一种从曲料中提取丝状真菌总rna的方法 | |
RU2104300C1 (ru) | Способ получения белкового гидролизата дрожжевой биомассы | |
CN110004197A (zh) | 一种高效微生物发酵制备壳聚糖的方法 | |
RU2388821C2 (ru) | Способ получения панкреатической рибонуклеазы | |
CN112080487B (zh) | 一种羊胰酶制备方法 | |
RU2781287C1 (ru) | Способ получения микробного протеина из микробной биомассы продуцента метанокисляющих бактерий ферментативным методом | |
CN108220280B (zh) | 利用酵母提取高纯核酸的方法及其产品和应用 | |
JP4050060B2 (ja) | 二枚貝類由来のセルラーゼ及びその製造方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CP02 | Change in the address of a patent holder |
Address after: 223001 No.58 Hefei Road, qingjiangpu District, Huai'an City, Jiangsu Province Patentee after: HUAIAN MAIDESEN PHARMACEUTICAL Co.,Ltd. Address before: 223001 No.2 Zhujiang Road, qingjiangpu District, Huai'an City, Jiangsu Province Patentee before: HUAIAN MAIDESEN PHARMACEUTICAL Co.,Ltd. |
|
CP02 | Change in the address of a patent holder | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Preparation of trypsin Effective date of registration: 20211226 Granted publication date: 20210302 Pledgee: Huaian Qingjiang puyintai financing Company limited by guarantee Pledgor: HUAIAN MAIDESEN PHARMACEUTICAL Co.,Ltd. Registration number: Y2021320000412 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20230116 Granted publication date: 20210302 Pledgee: Huaian Qingjiang puyintai financing Company limited by guarantee Pledgor: HUAIAN MAIDESEN PHARMACEUTICAL Co.,Ltd. Registration number: Y2021320000412 |
|
CP03 | Change of name, title or address | ||
CP03 | Change of name, title or address |
Address after: 223005 No. 58, Hefei Road, Huai'an City, Jiangsu Province Patentee after: Jiangsu Madsen Pharmaceutical Co.,Ltd. Country or region after: China Address before: 223001 No.58 Hefei Road, qingjiangpu District, Huai'an City, Jiangsu Province Patentee before: HUAIAN MAIDESEN PHARMACEUTICAL Co.,Ltd. Country or region before: China |