CN109790561B - 利用基本上不含凝固蛋白原的鲎阿米巴样细胞溶解物检测内毒素的方法 - Google Patents
利用基本上不含凝固蛋白原的鲎阿米巴样细胞溶解物检测内毒素的方法 Download PDFInfo
- Publication number
- CN109790561B CN109790561B CN201780048853.4A CN201780048853A CN109790561B CN 109790561 B CN109790561 B CN 109790561B CN 201780048853 A CN201780048853 A CN 201780048853A CN 109790561 B CN109790561 B CN 109790561B
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- China
- Prior art keywords
- coagulogen
- amoeba
- cell lysate
- lal
- endotoxin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/10—Anilides
- C12Q2337/12—Para-Nitroanilides p-NA
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
- G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- G01N2400/50—Lipopolysaccharides; LPS
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Water Supply & Treatment (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662370493P | 2016-08-03 | 2016-08-03 | |
| US62/370,493 | 2016-08-03 | ||
| PCT/US2017/045137 WO2018026941A1 (en) | 2016-08-03 | 2017-08-02 | Method of detecting an endotoxin using limulus amebocyte lysate substantially free of coagulogen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109790561A CN109790561A (zh) | 2019-05-21 |
| CN109790561B true CN109790561B (zh) | 2024-02-06 |
Family
ID=61069187
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201780048853.4A Active CN109790561B (zh) | 2016-08-03 | 2017-08-02 | 利用基本上不含凝固蛋白原的鲎阿米巴样细胞溶解物检测内毒素的方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US11156614B2 (https=) |
| EP (2) | EP3494226B1 (https=) |
| JP (1) | JP7044222B2 (https=) |
| KR (1) | KR102561368B1 (https=) |
| CN (1) | CN109790561B (https=) |
| IL (1) | IL264464B2 (https=) |
| SG (1) | SG11201900767VA (https=) |
| WO (1) | WO2018026941A1 (https=) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9950282B2 (en) | 2012-03-15 | 2018-04-24 | Flodesign Sonics, Inc. | Electronic configuration and control for acoustic standing wave generation |
| US9458450B2 (en) | 2012-03-15 | 2016-10-04 | Flodesign Sonics, Inc. | Acoustophoretic separation technology using multi-dimensional standing waves |
| US10967298B2 (en) | 2012-03-15 | 2021-04-06 | Flodesign Sonics, Inc. | Driver and control for variable impedence load |
| US10704021B2 (en) | 2012-03-15 | 2020-07-07 | Flodesign Sonics, Inc. | Acoustic perfusion devices |
| WO2015105955A1 (en) | 2014-01-08 | 2015-07-16 | Flodesign Sonics, Inc. | Acoustophoresis device with dual acoustophoretic chamber |
| US11377651B2 (en) | 2016-10-19 | 2022-07-05 | Flodesign Sonics, Inc. | Cell therapy processes utilizing acoustophoresis |
| US11708572B2 (en) | 2015-04-29 | 2023-07-25 | Flodesign Sonics, Inc. | Acoustic cell separation techniques and processes |
| US11021699B2 (en) | 2015-04-29 | 2021-06-01 | FioDesign Sonics, Inc. | Separation using angled acoustic waves |
| US11459540B2 (en) | 2015-07-28 | 2022-10-04 | Flodesign Sonics, Inc. | Expanded bed affinity selection |
| US11474085B2 (en) | 2015-07-28 | 2022-10-18 | Flodesign Sonics, Inc. | Expanded bed affinity selection |
| US11214789B2 (en) | 2016-05-03 | 2022-01-04 | Flodesign Sonics, Inc. | Concentration and washing of particles with acoustics |
| US11085035B2 (en) | 2016-05-03 | 2021-08-10 | Flodesign Sonics, Inc. | Therapeutic cell washing, concentration, and separation utilizing acoustophoresis |
| JP7044222B2 (ja) | 2016-08-03 | 2022-03-30 | ロンザ ウォーカーズヴィル，インコーポレーテッド | 実質的にコアギュローゲンを含まないリムルスアメボサイトライセートを使用してエンドトキシンを検出する方法 |
| CA3041517A1 (en) | 2016-10-19 | 2018-04-26 | Flodesign Sonics, Inc. | Affinity cell extraction by acoustics |
| JP7507560B2 (ja) * | 2017-01-11 | 2024-06-28 | ロンザ ウォーカーズヴィル，インコーポレーテッド | コアギュローゲンを含まない、清澄化リムルスアメボサイトライセート |
| WO2019118921A1 (en) | 2017-12-14 | 2019-06-20 | Flodesign Sonics, Inc. | Acoustic transducer drive and controller |
| GB201800303D0 (en) * | 2018-01-09 | 2018-02-21 | Univ Plymouth | Water quality testing |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5310657A (en) * | 1989-10-30 | 1994-05-10 | Whittaker Bioproducts, Inc. | Kinetic assay for endotoxin using limulus amebocyte lysate and chromogenic substrate |
| CN1105757A (zh) * | 1993-02-26 | 1995-07-26 | 生化学工业株式会社 | 用于内毒素测定的试剂以及使用该试剂进行测定的方法 |
| CN104155442A (zh) * | 2008-08-18 | 2014-11-19 | 拜奥泰克公司 | 增强的内毒素检测 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4322217A (en) | 1980-06-27 | 1982-03-30 | Mallinckrodt, Inc. | Process for preparing Limulus lysate |
| JP3122454B2 (ja) | 1990-09-27 | 2001-01-09 | 生化学工業株式会社 | カブトガニ・アメボサイト・ライセートの調製法 |
| US5208148A (en) | 1990-12-07 | 1993-05-04 | Molecular Probes, Inc. | Lipophilic fluorescent glycosidase substrates |
| US5242805A (en) | 1991-08-23 | 1993-09-07 | Molecular Probes, Inc. | Long wavelength lipophilic fluorogenic glycosidase substrates |
| WO1993004192A1 (en) | 1991-08-23 | 1993-03-04 | Molecular Probes, Inc. | Use of haloalkyl derivatives of reporter molecules to analyze metabolic activity in cells |
| US5576424A (en) | 1991-08-23 | 1996-11-19 | Molecular Probes, Inc. | Haloalkyl derivatives of reporter molecules used to analyze metabolic activity in cells |
| US5830912A (en) | 1996-11-15 | 1998-11-03 | Molecular Probes, Inc. | Derivatives of 6,8-difluoro-7-hydroxycoumarin |
| US5773236A (en) | 1997-04-25 | 1998-06-30 | Molecule Probes, Inc. | Assay for glutathiane transferase using polyhaloaryl-substituted reporter molecules |
| JP2010522540A (ja) | 2007-03-21 | 2010-07-08 | アイディー バイオメディカル コーポレイション オブ ケベック | キメラ抗原 |
| WO2009063840A1 (ja) | 2007-11-12 | 2009-05-22 | Hiroshima University | エンドトキシンの濃度測定方法および濃度測定用キット |
| MY155541A (en) | 2010-10-29 | 2015-10-30 | Universiti Malaysia Terengganu | Process for the preparation of amoebocyte lysate from the haemolymph of the horseshoe crab |
| CN102305788A (zh) | 2010-11-22 | 2012-01-04 | 天津市一瑞生物工程有限公司 | 革兰阴性菌脂多糖(内毒素)比色检测试剂盒的制备及其使用方法 |
| JP7044222B2 (ja) | 2016-08-03 | 2022-03-30 | ロンザ ウォーカーズヴィル，インコーポレーテッド | 実質的にコアギュローゲンを含まないリムルスアメボサイトライセートを使用してエンドトキシンを検出する方法 |
| JP7507560B2 (ja) * | 2017-01-11 | 2024-06-28 | ロンザ ウォーカーズヴィル，インコーポレーテッド | コアギュローゲンを含まない、清澄化リムルスアメボサイトライセート |
| SG11201912888TA (en) * | 2017-06-28 | 2020-01-30 | Lonza Walkersville Inc | Cartridge for endotoxin detection |
-
2017
- 2017-08-02 JP JP2019504062A patent/JP7044222B2/ja active Active
- 2017-08-02 KR KR1020197005917A patent/KR102561368B1/ko active Active
- 2017-08-02 EP EP17837616.6A patent/EP3494226B1/en active Active
- 2017-08-02 IL IL264464A patent/IL264464B2/en unknown
- 2017-08-02 WO PCT/US2017/045137 patent/WO2018026941A1/en not_active Ceased
- 2017-08-02 SG SG11201900767VA patent/SG11201900767VA/en unknown
- 2017-08-02 EP EP21183657.2A patent/EP3943608B1/en active Active
- 2017-08-02 CN CN201780048853.4A patent/CN109790561B/zh active Active
- 2017-08-03 US US15/668,101 patent/US11156614B2/en active Active
-
2021
- 2021-09-23 US US17/482,918 patent/US11906521B2/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5310657A (en) * | 1989-10-30 | 1994-05-10 | Whittaker Bioproducts, Inc. | Kinetic assay for endotoxin using limulus amebocyte lysate and chromogenic substrate |
| CN1105757A (zh) * | 1993-02-26 | 1995-07-26 | 生化学工业株式会社 | 用于内毒素测定的试剂以及使用该试剂进行测定的方法 |
| CN104155442A (zh) * | 2008-08-18 | 2014-11-19 | 拜奥泰克公司 | 增强的内毒素检测 |
Non-Patent Citations (2)
| Title |
|---|
| "Endotoxemia: Methods of Detection and Clinical Correlates";JAMES C. HURLEY;《CLINICAL MICROBIOLOGY REVIEWS》;19950430;第8卷(第2期);第268-292页 * |
| "Fractionation of Limulus amebocyte lysate. Characterization of activation of the proclotting enzyme by an endotoxin-mediated activator";SHIN NAKAMURA et al.;《Biochimica et biophysica acta》;19821231;第707卷;摘要、第218-219页过渡段、第220页左栏至右栏第1段和表1 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2018026941A1 (en) | 2018-02-08 |
| US11156614B2 (en) | 2021-10-26 |
| IL264464B1 (en) | 2023-10-01 |
| KR102561368B1 (ko) | 2023-08-01 |
| IL264464A (en) | 2019-02-28 |
| EP3494226A4 (en) | 2020-03-18 |
| US11906521B2 (en) | 2024-02-20 |
| EP3943608B1 (en) | 2023-07-12 |
| EP3494226B1 (en) | 2021-09-22 |
| CN109790561A (zh) | 2019-05-21 |
| IL264464B2 (en) | 2024-02-01 |
| US20220074940A1 (en) | 2022-03-10 |
| KR20190037284A (ko) | 2019-04-05 |
| SG11201900767VA (en) | 2019-02-27 |
| JP2019526052A (ja) | 2019-09-12 |
| US20180038864A1 (en) | 2018-02-08 |
| EP3943608C0 (en) | 2023-07-12 |
| EP3494226A1 (en) | 2019-06-12 |
| EP3943608A1 (en) | 2022-01-26 |
| JP7044222B2 (ja) | 2022-03-30 |
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