CN109771528A - A kind of improvement eye is dry, eye is puckery, asthenopia cold compress eye sticker and preparation method thereof - Google Patents

Abstract

The invention discloses a kind of improvement eyes to do, eye is puckery, asthenopia cold compress eye sticker, including activity extract, menthol and hydrogel；The activity extract is the extract of Rhizoma Et Radix Notopterygii, tripterygium wilfordii, cordate houttuynia, honeysuckle, Radix Rehmanniae, radix saposhnikoviae, balsam pear, imperial tongue, aloe, coptis root, sword bean, fennel seeds, polygonum cuspidate and folium eucalypti；The weight ratio of the activity extract, menthol and hydrogel is 10:1~10:10~50.Dry, puckery, asthenopia the cold compress eye sticker of eye preparation method that the invention also discloses improvement eyes.The present invention improves that eye is dry, eye is puckery, asthenopia cold compress eye sticker the problems such as there are palliative, high recurrence rates, drug dependence easy to form for overcoming existing drug.

Description

A kind of improvement eye is dry, eye is puckery, asthenopia cold compress eye sticker and preparation method thereof

Technical field

The present invention relates to Traditional-Chinese-medicine-type preservative free eye care product technical fields more particularly to a kind of improvement eye to do, eye is puckery, asthenopia cold Eye sticker is applied, dry, puckery, asthenopia the cold compress eye sticker of eye preparation method that the invention further relates to a kind of improvement eyes.

Background technique

The dry also known as xerophthalmia of eye, refers to that tear matter caused by any reason or amount exception or dynamics are abnormal, leads to tear film Stability decline, and with ophthalmic uncomfortable and (or) the general name of a variety of diseases of ocular lesion tissue feature.Also known as angle conjunctiva is dry Dry disease.Phenomena such as eye is puckery to refer to that eyes have the dry and astringent, trace of blood, keep in dark place, and sheds tears, or even red and swollen, illustrate that your eyes have received Injury.Asthenopia is also known as visual fatigue, is a kind of ophthalmology common disease, and the eye caused by it is dry, eye is puckery, eye is sour swollen, and blurred vision is very The work and life of people are directly affected to visual impairment.Asthenopia is mainly since people usually concentrate to see TV, electricity When the electronic products screen such as brain or mobile phone, eyes number of winks is reduced, and causes tears Secretion that should reduce, while it is strong to flash fluorescent screen Caused by strong stimulation eyes.Asthenopia can also cause and aggravate various eye disease.The best mode of relieving eye strain is to allow eye Eyeball rest.

With the development of science and technology, electronic product is widely available, with eye eyeshield, oneself is become healthy hot spot concerned by people One of.The common sympton that eye is dry, eye is puckery, asthenopia is daily excess eye-using, especially stay up late for a long time with eye, strong light or Eye and close eye are used under low light condition, clinically to include that eyes are dry and astringent, are easy tired, eye itch common symptom, there have to be different Object sense, pain burning heat sensation, secretion is sticky, fear of wind, photophobia, very sensitive to environmental stimuli；Sometimes eyes are too dry, and basic tear is not Foot stimulates reflexive lacrimal secretion instead, and causes usually to shed tears；More severe case eyes can redness, hyperemia, keratinization, cornea Epithelium broken skin and there is filiform to stick, with the passing of time this damage can then cause angle conjunctive disorder, and will affect eyesight.

Currently, clinical treatment eye is dry, eye is puckery, asthenopia method mainly uses western medical treatment.The medication of western medical treatment institute Object includes tear substitutes, sodium hyaluronate, growth factor, bFGF bovine basic fibroblast growth factor, recombinant human epidermal life The long factor, anti-inflammatory agent and glucocorticoid.But existing improvement eye is dry, eye is puckery, asthenopia drug has the following deficiencies: now Some doctor trained in Western medicine class drugs are usually palliative, can short-term relief symptom, but high recurrence rate, drug dependence easy to form Deng.

Summary of the invention

For overcome the deficiencies in the prior art, one of the objects of the present invention is to provide a kind of improvement eyes to do, eye is puckery, eye is tired The cold compress eye sticker of labor, with for improving the symptoms such as dry, the puckery, asthenopia of eye of eye, overcoming existing drug, there are palliative, recurrences The problems such as rate is high, drug dependence easy to form, also can effectively avoid that effective component concentration existing for Chinese medicine is low, improves the time Long, the problems such as effect is poor, middle or short term medication is difficult to realize remission.

The second object of the present invention is to provide that a kind of improvement eye is dry, preparation method of puckery, asthenopia the cold compress eye sticker of eye, The improvement eye prepared by this method is dry, eye is puckery, asthenopia cold compress eye sticker is to be used to improve the diseases such as dry, the puckery, asthenopia of eye of eye Shape, overcome existing drug there are palliative, high recurrence rate, it is easy to form drug dependence the problems such as, also can effectively avoid Effective component concentration existing for Chinese medicine is low, improves the problems such as time is long, effect is poor, middle or short term medication is difficult to realize remission.

An object of the present invention adopts the following technical scheme that realization:

A kind of improvement eye is dry, eye is puckery, asthenopia cold compress eye sticker, including activity extract, menthol and hydrogel；

The activity extract is Rhizoma Et Radix Notopterygii, tripterygium wilfordii, cordate houttuynia, honeysuckle, Radix Rehmanniae, radix saposhnikoviae, balsam pear, imperial tongue, aloe, Huang Lotus, sword bean, fennel seeds, polygonum cuspidate and folium eucalypti extract；

The weight ratio of the activity extract, menthol and hydrogel is 10:1~10:10~50.

Further, by weight, the Rhizoma Et Radix Notopterygii is 3~15 parts, and the tripterygium wilfordii is 2~10 parts, the cordate houttuynia It is 6~20 parts, the honeysuckle is 4~16 parts, and the Radix Rehmanniae is 1~10 part, and the radix saposhnikoviae is 2~8 parts, and the balsam pear is 3 ~12 parts, the dragon tongue is 5~12 parts, and the aloe is 3~10 parts, and the coptis root is 5~20 parts, and the sword bean is 3~15 Part, the fennel seeds are 4~18 parts, and the polygonum cuspidate is 6~24 parts, and the folium eucalypti is 6~15 parts.

Further, by weight, the Rhizoma Et Radix Notopterygii is 5~10 parts, and the tripterygium wilfordii is 4~8 parts, and the cordate houttuynia is 10~15 parts, the honeysuckle is 8~12 parts, and the Radix Rehmanniae is 3~7 parts, and the radix saposhnikoviae is 4~6 parts, and the balsam pear is 5~9 Part, the dragon tongue is 7~10 parts, and the aloe is 5~9 parts, and the coptis root is 8~16 parts, and the sword bean is 6~12 parts, institute Stating fennel seeds is 9~15 parts, and the polygonum cuspidate is 12~20 parts, and the folium eucalypti is 9~12 parts.

Further, by weight, the Rhizoma Et Radix Notopterygii is 7 parts, and the tripterygium wilfordii is 6 parts, and the cordate houttuynia is 13 parts, institute Stating honeysuckle is 9 parts, and the Radix Rehmanniae is 5 parts, and the radix saposhnikoviae is 5 parts, and the balsam pear is 7 parts, and the dragon tongue is 9 parts, the reed Luxuriant growth is 7 parts, and the coptis root is 12 parts, and the sword bean is 9 parts, and the fennel seeds are 12 parts, and the polygonum cuspidate is 16 parts, the folium eucalypti It is 11 parts.

The second object of the present invention adopts the following technical scheme that realization:

It is described in any of the above embodiments to improve that eye is dry, preparation method of puckery, asthenopia the cold compress eye sticker of eye, including following step It is rapid:

Stock: weighing Rhizoma Et Radix Notopterygii, tripterygium wilfordii, cordate houttuynia, honeysuckle, Radix Rehmanniae and windproof constituent component A, weigh balsam pear, imperial tongue, Aloe and coptis root constituent component B weigh sword bean, fennel seeds, polygonum cuspidate and folium eucalypti constituent component C, component A, B and C are pulverized, Cross 10~40 meshes；

It prepares the first extract: 75~95% ethyl alcohol is added with the solid-liquid ratios of 1:5~15 in component A, stirring, impregnate 20~ After 80min, concentrate is concentrated under reduced pressure into 0.5~2.5h of refluxing extraction at 70~85 DEG C, filtering, filtrate, adds anhydrous second Alcohol standing, Precipitation take supernatant heating to be concentrated into paste, the first extract are made；

It prepares the second extract: 75~95% ethyl alcohol is added with the solid-liquid ratios of 1:4~10 in component B, stirring, impregnate 20~ After 60min, 1~5h of refluxing extraction at 60~70 DEG C, filtering, filtrate are concentrated under reduced pressure to give concentrate, concentrate with 0.5~ 5% dissolving with hydrochloric acid takes lysate part to be adjusted to pH8.6~10.4 with ammonium hydroxide, continuously adds the two of 2~5 times of lysate volume Dichloromethane layer is concentrated under reduced pressure into powdered, obtained second extract for chloromethanes, mixing, layering；

Prepare third extract: by component C with the solid-liquid ratios of 1:2~5 be added 0.5~2mol/L acetum and with Dehydrated alcohol is added in the solid-liquid ratio of 1:5~15, and 100~500W ultrasonic extraction time is 10~60min, 3000~8000r/min Be centrifuged 3~15min, clear liquid is condensed into medicinal extract shape after centrifugation, by the chloroform refluxing extraction 20 of 2~5 times of volumes of medicinal extract~ Third extract is made in being concentrated into paste on water-bath in 90min, extracting solution；

Preparations shaping: the first extract, the second extract, third extract, menthol and hydrogel are uniformly mixed, and are used Glycerol is modulated into emulsifiable paste shape, and being made improves that eye is dry, eye is puckery, asthenopia cold compress eye sticker.

Further, in preparing the first extract step, 80% ethyl alcohol is added with the solid-liquid ratio of 1:10 in component A, is stirred After mixing, impregnating 50min, refluxing extraction 1.5h at 78 DEG C, double gauze filtering, filtrate are transferred in vacuum rotary evaporator and depressurize It is condensed into concentrate, thickening temperature is 68 DEG C, and vacuum degree is -0.04MPa；

The dehydrated alcohol that 3 times of volumes of concentrate are added stands, is layered, and takes supernatant to be placed on 70 DEG C of thermostat water baths and is concentrated To paste, the first extract is made.

Further, in preparing the second extract step, 85% ethyl alcohol is added with the solid-liquid ratio of 1:8 in component B, is stirred After mixing, impregnating 30min, refluxing extraction 2.5h at 65 DEG C, filtering, filtrate are transferred in vacuum rotary evaporator and are concentrated under reduced pressure to give Concentrate, wherein thickening temperature is 55 DEG C, and vacuum degree is -0.08MPa.

Further, 1.5% dissolving with hydrochloric acid of concentrate, separates lysate part, takes lysate part ammonium hydroxide tune To pH9.4,4 times of lysate volume of methylene chloride is continuously added, dichloromethane layer is concentrated under reduced pressure into powder for mixing, layering The second extract is made in shape；

Wherein, it is 37 DEG C that temperature, which is concentrated under reduced pressure, and vacuum degree is -0.02MPa.

Further, in preparation third extract step, component C is added to the acetic acid of 1mol/L with the solid-liquid ratio of 1:3 Solution and dehydrated alcohol is added with the solid-liquid ratio of 1:10,300W ultrasonic extraction 35min, 6000r/min is centrifuged 8min, after centrifugation Clear liquid, which is transferred in vacuum rotary evaporator, is condensed into medicinal extract shape, wherein thickening temperature is 60 DEG C, and vacuum degree is -0.15MPa；

By the medicinal extract chloroform refluxing extraction 60min of 3.5 times of volumes, extracting solution is thick in being concentrated on 45 DEG C of thermostat water baths Paste.

Further, in preparations shaping step, the first extract, the second extract, third extract are mixed in advance Primary extract is made, adds menthol and hydrogel, is uniformly mixed；

Wherein, the weight ratio of primary extract, menthol and hydrogel is 10:3:20.

Compared with prior art, the beneficial effects of the present invention are:

(1) present invention improvement eye is dry, eye is puckery, asthenopia cold compress eye sticker can be effectively improved dry, puckery, asthenopia of eye of eye etc. Symptom, treated effect is high, reaches the therapeutic level of existing common medicine hydroxychloroquine sulfate piece.With existing common medicine hydroxychloroquine sulfate Piece compares, and the present invention improves the more excellent effect that eye is dry, eye is puckery, asthenopia cold compress eye sticker also has recurrence rate low.In addition, this Invention improve eye is dry, eye is puckery, asthenopia cold compress eye sticker using Chinese medical extract as effective component, it is nontoxic in therapeutic process, Side effect, by means of the endothermic effect of cold compress gel, moreover it is possible to siphon away excitement caused by eye is dry, eye is puckery rapidly, be brought for patient Refrigerant comfort is effectively relieved tingling sensation, restores crystalline lens and ciliary muscle elasticity.

(2) present invention improves that eye is dry, preparation method of puckery, asthenopia the cold compress eye sticker of eye, and pass through prepared by this method changes The effective component that kind eye is dry, eye is puckery, asthenopia cold compress eye sticker can be effectively retained various activity extracts, including in Chinese herbal medicine Acid-soluble ingredient (such as alkaloid), liposoluble constituent and other active constituents, be conducive to by means of a variety of extracting modes Active constituent sufficiently in release Chinese medicine further promotes the concentration of active constituent, finally combines by means of a variety of purification process The complex role of each effective component can effectively penetrate skin disease and act on subcutaneous eyeball, retina neural and musculature Deng, the various symptoms that guarantee rapid recovery eye is dry, eye is puckery, asthenopia causes, promotion drug component function and effect, when shortening acts on Between, long-time service does not cause side effect, and patient is facilitated to use.

Specific embodiment

In the following, being described further in conjunction with specific embodiment to the present invention, it should be noted that is do not collided Under the premise of, new embodiment can be formed between various embodiments described below or between each technical characteristic in any combination.

Hereinafter, by Examples 1 to 5, the present invention will be described in detail improves that eye is dry, preparation of puckery, asthenopia the cold compress eye sticker of eye Process.

Embodiment 1

Material preparation step: weigh the Rhizoma Et Radix Notopterygii dried be 3g, tripterygium wilfordii 10g, cordate houttuynia 20g, honeysuckle 4g, Radix Rehmanniae 1g and anti- Wind 2g constituent component A.Weigh the balsam pear 12g dried, imperial tongue 5g, aloe 10g and coptis root 5g constituent component B.It weighs and dries Sword bean 15g, fennel seeds 4g, polygonum cuspidate 24g and folium eucalypti 6g constituent component C.

It prepares the first extract step: component A is transferred in round-bottomed flask, according to solid-liquid ratio (i.e. the ratio between quality volume Value) it is 1:5, the ethyl alcohol that mass fraction is 95% is added into round-bottomed flask, and stirring rod is set and stirs 80min in a round bottom flask (or round-bottomed flask is placed on shaking table between oscillating phase is seasonable).Round-bottomed flask is connected into condensing reflux pipeline and is prepared into one Set reflux extraction element, round-bottomed flask is placed in 85 DEG C of thermostat water baths, and rotation round-bottomed flask carries out refluxing extraction 0.5h.It returns After flowing extraction process, mixed liquor is filtered by double gauze, filters out the dregs of a decoction.Filtrate is collected, filtrate is transferred to vacuum In Rotary Evaporators, there is wall built-up and (opens in rotary evaporation to extracting solution under the conditions of vacuum degree is -0.01MPa and temperature is 75 DEG C Begin to occur sticky) phenomenon when stop, then dehydrated alcohol is added into round-bottomed flask, the addition volume of dehydrated alcohol is concentrated liquid Long-pending 3 times vibrate round-bottomed flask and concentrate are uniformly mixed with dehydrated alcohol, stand for 24 hours, be layered, dehydrated alcohol The contamination precipitations such as protein, polysaccharide, inorganic salts are precipitated, the effective component being achieved in purified components A.Aspirate supernatant is simultaneously It is transferred on 65 DEG C of thermostat water baths, heating, stirring are concentrated into and become paste, and the first extract is made as a result,.

It prepares the second extract step: component B is transferred in round-bottomed flask, according to solid-liquid ratio (i.e. the ratio between quality volume Value) it is 1:5, the ethyl alcohol that mass fraction is 95% is added into round-bottomed flask, and stirring rod is set and stirs 60min in a round bottom flask (or round-bottomed flask is placed on shaking table between oscillating phase is seasonable).Round-bottomed flask is connected into condensing reflux pipeline and is prepared into one Set reflux extraction element, round-bottomed flask is placed in the thermostat water bath that temperature is 70 DEG C, and rotation round-bottomed flask carries out reflux and mentions Take 1h.After refluxing extraction process, mixed liquor is filtered by double gauze, filters out the dregs of a decoction.Filtrate is collected, filtrate is shifted Into vacuum rotary evaporator, rotary evaporation to extracting solution is hung under the conditions of vacuum degree is -0.02MPa and temperature is 60 DEG C Wall (starts to occur sticky) to stop when phenomenon, and concentrate is made.5% hydrochloric acid solution, concentration are added into concentrate obtained The ratio between volume of liquid and hydrochloric acid solution is 1:1, and the stirring of Bian Tianjia hydrochloric acid solution side stands 1h, obtains acid dissolution part and non-acid Part is dissolved, taking acid dissolution part pH value is 10.6 ammonium hydroxide tune pH8.6, continuously adds methylene chloride, wherein acid dissolution portion Dividing with the volume ratio of methylene chloride is 1:2, oscillation mixing, stratification.It draws dichloromethane layer and is transferred to vacuum rotating steaming Hair instrument, setting evaporating temperature are 33 DEG C, and vacuum degree is -0.08MPa, are evaporated under reduced pressure into powdered, obtained second extract.

Preparation third extract step: component C is transferred in round-bottomed flask, according to solid-liquid ratio (i.e. the ratio between quality volume Value) be 1:5 amount add the acetum of 0.5mol/L into round-bottomed flask, according still further to solid-liquid ratio (i.e. the ratio between quality volume be worth) Dehydrated alcohol is added for the amount of 1:5, oscillation mixes.Round-bottomed flask is transferred in Ultrasound Instrument, adjusting ultrasonic power is 500W, is surpassed Sound extracts 10min.After ultrasonic extraction process, extracting solution is filtered by double gauze, the dregs of a decoction is filtered out, collects filtrate.It will Filtrate is transferred in centrifuge tube, and centrifuge tube, which is placed in a centrifuge, to be centrifuged, centrifuge RPMs 3000r/min, and centrifugation time is 15min.Centrifuge tube is taken out after centrifugation, absorption supernatant liquor, which is transferred in vacuum rotary evaporator, is condensed into medicinal extract shape, In, thickening temperature is 60 DEG C, and vacuum degree is -0.15MPa.The chloroform for adding 2 times of supernatant liquor volumes into concentrate bottle again, will be dense Contracting bottle is placed in the thermostat water bath that temperature is 45 DEG C, and spin concentration bottle carries out refluxing extraction 20min.After refluxing extraction, Extracting solution is transferred in triangular flask and triangular flask is placed on the thermostat water bath that temperature is 45 DEG C, in heating evaporation chloroform Third extract is made until chloroform evaporated is complete and is concentrated into paste (or blocky) in stirring.

Preparations shaping step: the first extract, the second extract and third extract are stirred and evenly mixed in advance, primary is made Medicinal extract, then primary extract, menthol and hydrogel are uniformly mixed, it is modulated into emulsifiable paste shape with glycerol, obtains improvement of the present invention Eye is dry, eye is puckery, asthenopia cold compress gel.By above-mentioned improvement eye is dry, eye is puckery, asthenopia cold compress gel is spread evenly across 50 On non-woven fabrics, release paper is covered, obtained present invention improvement eye is dry, eye is puckery, asthenopia cold compress eye sticker.Wherein, primary extract, thin The weight ratio of lotus brain and hydrogel is 10:1:50.

Embodiment 2

Material preparation step: weigh the Rhizoma Et Radix Notopterygii dried be 10g, tripterygium wilfordii 8g, cordate houttuynia 10g, honeysuckle 12g, Radix Rehmanniae 3g and Windproof 6g constituent component A.Weigh the balsam pear 9g dried, imperial tongue 10g, aloe 5g and coptis root 16g constituent component B.It weighs and dries Sword bean 6g, fennel seeds 9g, polygonum cuspidate 12g and folium eucalypti 12g constituent component C.

It prepares the first extract step: component A is transferred in round-bottomed flask, according to solid-liquid ratio (i.e. the ratio between quality volume Value) it is 1:8, the ethyl alcohol that mass fraction is 90% is added into round-bottomed flask, and stirring rod is set and stirs 65min in a round bottom flask (or round-bottomed flask is placed on shaking table between oscillating phase is seasonable).Round-bottomed flask is connected into condensing reflux pipeline and is prepared into one Set reflux extraction element, round-bottomed flask is placed in 82 DEG C of thermostat water baths, and rotation round-bottomed flask carries out refluxing extraction 1h.Reflux After extraction process, mixed liquor is filtered by double gauze, filters out the dregs of a decoction.Filtrate is collected, filtrate is transferred to vacuum rotation Turn in evaporimeter, wall built-up (beginning occurs in rotary evaporation to extracting solution under the conditions of vacuum degree is -0.02MPa and temperature is 72 DEG C Occur sticky) phenomenon when stop, then dehydrated alcohol is added into round-bottomed flask, the addition volume of dehydrated alcohol is the volume of the concentrated liquid 4 times, oscillation round-bottomed flask simultaneously concentrate is uniformly mixed with dehydrated alcohol, standing for 24 hours, be layered, dehydrated alcohol makes The contamination precipitations such as protein, polysaccharide, inorganic salts are precipitated, the effective component being achieved in purified components A.Aspirate supernatant simultaneously turns It moves on 68 DEG C of thermostat water baths, heating, stirring are concentrated into and become paste, and the first extract is made as a result,.

It prepares the second extract step: component B is transferred in round-bottomed flask, according to solid-liquid ratio (i.e. the ratio between quality volume Value) it is 1:10, the ethyl alcohol that mass fraction is 80% is added into round-bottomed flask, and stirring rod is set and is stirred in a round bottom flask 45min (or round-bottomed flask is placed on shaking table between oscillating phase is seasonable).Round-bottomed flask is connected into the pipeline system of condensing reflux Standby to be placed in round-bottomed flask in the thermostat water bath that temperature is 68 DEG C at a set of refluxing extraction device, rotation round-bottomed flask carries out Refluxing extraction 1.5h.After refluxing extraction process, mixed liquor is filtered by double gauze, filters out the dregs of a decoction.Filtrate is collected, it will Filtrate is transferred in vacuum rotary evaporator, and rotary evaporation extremely extracts under the conditions of vacuum degree is -0.05MPa and temperature is 58 DEG C There is wall built-up and (starts to occur sticky) to stop when phenomenon in liquid, and concentrate is made.Into concentrate obtained, the hydrochloric acid of addition 4% is molten The ratio between volume of liquid, concentrate and hydrochloric acid solution is 1:2, and the stirring of Bian Tianjia hydrochloric acid solution side stands 1h, obtains acid dissolution part With non-acid dissolution part, taking acid dissolution part pH value is 11.2 ammonium hydroxide tune pH9.0, continuously adds methylene chloride, wherein acid The volume ratio of dissolution part and methylene chloride is 1:3, oscillation mixing, stratification.It draws dichloromethane layer and is transferred to vacuum Rotary Evaporators, it is 35 DEG C that evaporating temperature, which is arranged, and vacuum degree is -0.05MPa, is evaporated under reduced pressure into powdered, obtained second extraction Object.

Preparation third extract step: component C is transferred in round-bottomed flask, according to solid-liquid ratio (i.e. the ratio between quality volume Value) be 1:4 amount add 0.75mol/L acetum into round-bottomed flask, according still further to solid-liquid ratio (i.e. the ratio between quality volume It is worth) dehydrated alcohol is added for the amount of 1:8, oscillation mixes.Round-bottomed flask is transferred in Ultrasound Instrument, adjusting ultrasonic power is 400W, ultrasonic extraction 25min.After ultrasonic extraction process, extracting solution is filtered by double gauze, the dregs of a decoction is filtered out, collects Filtrate.Filtrate is transferred in centrifuge tube, centrifuge tube, which is placed in a centrifuge, to be centrifuged, centrifuge RPMs 4500r/min, centrifugation Time is 10min.Centrifuge tube is taken out after centrifugation, absorption supernatant liquor, which is transferred in vacuum rotary evaporator, is condensed into medicinal extract Shape, wherein thickening temperature is 70 DEG C, and vacuum degree is -0.15MPa.The chlorine of 3 times of supernatant liquor volumes is added into concentrate bottle again It is imitative, concentrate bottle is placed in the thermostat water bath that temperature is 50 DEG C, spin concentration bottle carries out refluxing extraction 35min.Refluxing extraction After, extracting solution is transferred in triangular flask and triangular flask is placed on the thermostat water bath that temperature is 55 DEG C, side heating is steamed The stirring of chloroform side is sent out, until chloroform evaporated is complete and is concentrated into paste (or blocky), third extract is made.

Preparations shaping step: the first extract, the second extract and third extract are stirred and evenly mixed in advance, primary is made Medicinal extract, then primary extract, menthol and hydrogel are uniformly mixed, it is modulated into emulsifiable paste shape with glycerol, obtains improvement of the present invention Eye is dry, eye is puckery, asthenopia cold compress gel.By above-mentioned improvement eye is dry, eye is puckery, asthenopia cold compress gel is spread evenly across 50 On non-woven fabrics, release paper is covered, obtained present invention improvement eye is dry, eye is puckery, asthenopia cold compress eye sticker.Wherein, primary extract, thin The weight ratio of lotus brain and hydrogel is 10:5:30.

Embodiment 3

Material preparation step: weigh the Rhizoma Et Radix Notopterygii dried be 7g, tripterygium wilfordii 6g, cordate houttuynia 13g, honeysuckle 9g, Radix Rehmanniae 5g and anti- Wind 5g constituent component A.Weigh the balsam pear 7g dried, imperial tongue 9g, aloe 7g and coptis root 12g constituent component B.Weigh the knife dried Beans 9g, fennel seeds 12g, polygonum cuspidate 16g and folium eucalypti 11g constituent component C.

It prepares the first extract step: component A is transferred in round-bottomed flask, according to solid-liquid ratio (i.e. the ratio between quality volume Value) it is 1:10, the ethyl alcohol that mass fraction is 80% is added into round-bottomed flask, and stirring rod is set and is stirred in a round bottom flask 50min (or round-bottomed flask is placed on shaking table between oscillating phase is seasonable).Round-bottomed flask is connected into the pipeline system of condensing reflux Standby that round-bottomed flask is placed in 78 DEG C of thermostat water baths at a set of refluxing extraction device, rotation round-bottomed flask carries out refluxing extraction 1.5h.After refluxing extraction process, mixed liquor is filtered by double gauze, filters out the dregs of a decoction.Filtrate is collected, filtrate is shifted Into vacuum rotary evaporator, rotary evaporation to extracting solution is hung under the conditions of vacuum degree is -0.04MPa and temperature is 68 DEG C Wall (starts to occur sticky) to stop when phenomenon, then dehydrated alcohol is added into round-bottomed flask, and the addition volume of dehydrated alcohol is dense 5 times of contracting liquid product vibrate round-bottomed flask and concentrate are uniformly mixed with dehydrated alcohol, stand for 24 hours, be layered, nothing The contamination precipitations such as protein, polysaccharide, inorganic salts are precipitated in water-ethanol, the effective component being achieved in purified components A.In absorption Clear liquid is simultaneously transferred on 70 DEG C of thermostat water baths, and heating, stirring are concentrated into and become paste, and the first extract is made as a result,.

It prepares the second extract step: component B is transferred in round-bottomed flask, according to solid-liquid ratio (i.e. the ratio between quality volume Value) it is 1:8, the ethyl alcohol that mass fraction is 85% is added into round-bottomed flask, and stirring rod is set and stirs 30min in a round bottom flask (or round-bottomed flask is placed on shaking table between oscillating phase is seasonable).Round-bottomed flask is connected into condensing reflux pipeline and is prepared into one Set reflux extraction element, round-bottomed flask is placed in the thermostat water bath that temperature is 65 DEG C, and rotation round-bottomed flask carries out reflux and mentions Take 2.5h.After refluxing extraction process, mixed liquor is filtered by double gauze, filters out the dregs of a decoction.Filtrate is collected, filtrate is turned It moves in vacuum rotary evaporator, rotary evaporation to extracting solution occurs under the conditions of vacuum degree is -0.08MPa and temperature is 55 DEG C Wall built-up (starts to occur sticky) to stop when phenomenon, and concentrate is made.2.5% hydrochloric acid solution is added into concentrate obtained, The ratio between volume of concentrate and hydrochloric acid solution is 1:2.5, and the stirring of Bian Tianjia hydrochloric acid solution side stands 1h, obtains acid dissolution part With non-acid dissolution part, taking acid dissolution part pH value is 11.2 ammonium hydroxide tune pH9.4, continuously adds methylene chloride, wherein acid The volume ratio of dissolution part and methylene chloride is 1:4, oscillation mixing, stratification.It draws dichloromethane layer and is transferred to vacuum Rotary Evaporators, it is 37 DEG C that evaporating temperature, which is arranged, and vacuum degree is -0.02MPa, is evaporated under reduced pressure into powdered, obtained second extraction Object.

Preparation third extract step: component C is transferred in round-bottomed flask, according to solid-liquid ratio (i.e. the ratio between quality volume Value) be 1:3 amount add 1mol/L acetum into round-bottomed flask, be according still further to solid-liquid ratio (i.e. the ratio between quality volume value) The amount of 1:10 adds dehydrated alcohol, and oscillation mixes.Round-bottomed flask is transferred in Ultrasound Instrument, adjusting ultrasonic power is 300W, is surpassed Sound extracts 35min.After ultrasonic extraction process, extracting solution is filtered by double gauze, the dregs of a decoction is filtered out, collects filtrate.It will Filtrate is transferred in centrifuge tube, and centrifuge tube, which is placed in a centrifuge, to be centrifuged, centrifuge RPMs 6000r/min, and centrifugation time is 8min.Centrifuge tube is taken out after centrifugation, absorption supernatant liquor, which is transferred in vacuum rotary evaporator, is condensed into medicinal extract shape, In, thickening temperature is 60 DEG C, and vacuum degree is -0.15MPa.The chloroform of 3.5 times of supernatant liquor volumes is added into concentrate bottle again, it will Concentrate bottle is placed in the thermostat water bath that temperature is 55 DEG C, and spin concentration bottle carries out refluxing extraction 60min.Refluxing extraction terminates Afterwards, extracting solution is transferred in triangular flask and triangular flask is placed on the thermostat water bath that temperature is 60 DEG C, side heating evaporation chlorine Third extract is made until chloroform evaporated is complete and is concentrated into paste (or blocky) in imitative side stirring.

Preparations shaping step: the first extract, the second extract and third extract are stirred and evenly mixed in advance, primary is made Medicinal extract, then primary extract, menthol and hydrogel are uniformly mixed, it is modulated into emulsifiable paste shape with glycerol, obtains improvement of the present invention Eye is dry, eye is puckery, asthenopia cold compress gel.By above-mentioned improvement eye is dry, eye is puckery, asthenopia cold compress gel is spread evenly across 50 On non-woven fabrics, release paper is covered, obtained present invention improvement eye is dry, eye is puckery, asthenopia cold compress eye sticker.Wherein, primary extract, thin The weight ratio of lotus brain and hydrogel is 10:3:20.

Embodiment 4

Material preparation step: weigh the Rhizoma Et Radix Notopterygii dried be 5g, tripterygium wilfordii 4g, cordate houttuynia 15g, honeysuckle 8g, Radix Rehmanniae 7g and anti- Wind 4g constituent component A.Weigh the balsam pear 5g dried, imperial tongue 7g, aloe 9g and coptis root 8g constituent component B.Weigh the knife dried Beans 12g, fennel seeds 15g, polygonum cuspidate 20g and folium eucalypti 9g constituent component C.

It prepares the first extract step: component A is transferred in round-bottomed flask, according to solid-liquid ratio (i.e. the ratio between quality volume Value) it is 1:12, the ethyl alcohol that mass fraction is 85% is added into round-bottomed flask, and stirring rod is set and is stirred in a round bottom flask 35min (or round-bottomed flask is placed on shaking table between oscillating phase is seasonable).Round-bottomed flask is connected into the pipeline system of condensing reflux Standby that round-bottomed flask is placed in 75 DEG C of thermostat water baths at a set of refluxing extraction device, rotation round-bottomed flask carries out refluxing extraction 2h.After refluxing extraction process, mixed liquor is filtered by double gauze, filters out the dregs of a decoction.Filtrate is collected, filtrate is transferred to In vacuum rotary evaporator, there is wall built-up in rotary evaporation to extracting solution under the conditions of vacuum degree is -0.05MPa and temperature is 65 DEG C Stop when (starting to occur sticky) phenomenon, then add dehydrated alcohol into round-bottomed flask, the addition volume of dehydrated alcohol is concentration 6 times of liquid product vibrate round-bottomed flask and concentrate are uniformly mixed with dehydrated alcohol, stand for 24 hours, be layered, anhydrous The contamination precipitations such as protein, polysaccharide, inorganic salts are precipitated in ethyl alcohol, the effective component being achieved in purified components A.Draw supernatant Liquid is simultaneously transferred on 72 DEG C of thermostat water baths, and heating, stirring are concentrated into and become paste, and the first extract is made as a result,.

It prepares the second extract step: component B is transferred in round-bottomed flask, according to solid-liquid ratio (i.e. the ratio between quality volume Value) it is 1:12, the ethyl alcohol that mass fraction is 90% is added into round-bottomed flask, and stirring rod is set and is stirred in a round bottom flask 25min (or round-bottomed flask is placed on shaking table between oscillating phase is seasonable).Round-bottomed flask is connected into the pipeline system of condensing reflux Standby to be placed in round-bottomed flask in the thermostat water bath that temperature is 62 DEG C at a set of refluxing extraction device, rotation round-bottomed flask carries out Refluxing extraction 3.5h.After refluxing extraction process, mixed liquor is filtered by double gauze, filters out the dregs of a decoction.Filtrate is collected, it will Filtrate is transferred in vacuum rotary evaporator, and rotary evaporation extremely extracts under the conditions of vacuum degree is -0.1MPa and temperature is 52 DEG C There is wall built-up and (starts to occur sticky) to stop when phenomenon in liquid, and concentrate is made.Into concentrate obtained, the hydrochloric acid of addition 4% is molten The ratio between volume of liquid, concentrate and hydrochloric acid solution is 1:4, and the stirring of Bian Tianjia hydrochloric acid solution side stands 1h, obtains acid dissolution part With non-acid dissolution part, taking acid dissolution part pH value is 12 ammonium hydroxide tune pH10.0, continuously adds methylene chloride, wherein acid The volume ratio of dissolution part and methylene chloride is 1:3.5, oscillation mixing, stratification.It draws dichloromethane layer and is transferred to true Empty Rotary Evaporators, setting evaporating temperature are 40 DEG C, and vacuum degree is -0.01MPa, are evaporated under reduced pressure at powdered, are made second and mention Take object.

Preparation third extract step: component C is transferred in round-bottomed flask, according to solid-liquid ratio (i.e. the ratio between quality volume Value) be 1:2.5 amount add 1.5mol/L acetum into round-bottomed flask, according still further to solid-liquid ratio (i.e. the ratio between quality volume It is worth) dehydrated alcohol is added for the amount of 1:12, oscillation mixes.Round-bottomed flask is transferred in Ultrasound Instrument, adjusting ultrasonic power is 200W, ultrasonic extraction 50min.After ultrasonic extraction process, extracting solution is filtered by double gauze, the dregs of a decoction is filtered out, collects Filtrate.Filtrate is transferred in centrifuge tube, centrifuge tube, which is placed in a centrifuge, to be centrifuged, centrifuge RPMs 7000r/min, centrifugation Time is 5min.Centrifuge tube is taken out after centrifugation, absorption supernatant liquor, which is transferred in vacuum rotary evaporator, is condensed into medicinal extract Shape, wherein thickening temperature is 70 DEG C, and vacuum degree is -0.15MPa.The chlorine of 4 times of supernatant liquor volumes is added into concentrate bottle again It is imitative, concentrate bottle is placed in the thermostat water bath that temperature is 60 DEG C, spin concentration bottle carries out refluxing extraction 45min.Refluxing extraction After, extracting solution is transferred in triangular flask and triangular flask is placed on the thermostat water bath that temperature is 65 DEG C, side heating is steamed The stirring of chloroform side is sent out, until chloroform evaporated is complete and is concentrated into paste (or blocky), third extract is made.

Preparations shaping step: the first extract, the second extract and third extract are stirred and evenly mixed in advance, primary is made Medicinal extract, then primary extract, menthol and hydrogel are uniformly mixed, it is modulated into emulsifiable paste shape with glycerol, obtains improvement of the present invention Eye is dry, eye is puckery, asthenopia cold compress gel.By above-mentioned improvement eye is dry, eye is puckery, asthenopia cold compress gel is spread evenly across 50 On non-woven fabrics, release paper is covered, obtained present invention improvement eye is dry, eye is puckery, asthenopia cold compress eye sticker.Wherein, primary extract, thin The weight ratio of lotus brain and hydrogel is 10:8:25.

Embodiment 5

Material preparation step: weigh the Rhizoma Et Radix Notopterygii dried be 15g, tripterygium wilfordii 2g, cordate houttuynia 6g, honeysuckle 16g, Radix Rehmanniae 10g and Windproof 8g constituent component A.Weigh the balsam pear 3g dried, imperial tongue 12g, aloe 3g and coptis root 20g constituent component B.It weighs and dries Sword bean 3g, fennel seeds 18g, polygonum cuspidate 6g and folium eucalypti 15g constituent component C.

It prepares the first extract step: component A is transferred in round-bottomed flask, according to solid-liquid ratio (i.e. the ratio between quality volume Value) it is 1:15, the ethyl alcohol that mass fraction is 75% is added into round-bottomed flask, and stirring rod is set and is stirred in a round bottom flask 20min (or round-bottomed flask is placed on shaking table between oscillating phase is seasonable).Round-bottomed flask is connected into the pipeline system of condensing reflux Standby that round-bottomed flask is placed in 70 DEG C of thermostat water baths at a set of refluxing extraction device, rotation round-bottomed flask carries out refluxing extraction 2.5h.After refluxing extraction process, mixed liquor is filtered by double gauze, filters out the dregs of a decoction.Filtrate is collected, filtrate is shifted Into vacuum rotary evaporator, rotary evaporation to extracting solution is hung under the conditions of vacuum degree is -0.08MPa and temperature is 60 DEG C Wall (starts to occur sticky) to stop when phenomenon, then dehydrated alcohol is added into round-bottomed flask, and the addition volume of dehydrated alcohol is dense 8 times of contracting liquid product vibrate round-bottomed flask and concentrate are uniformly mixed with dehydrated alcohol, stand for 24 hours, be layered, nothing The contamination precipitations such as protein, polysaccharide, inorganic salts are precipitated in water-ethanol, the effective component being achieved in purified components A.In absorption Clear liquid is simultaneously transferred on 75 DEG C of thermostat water baths, and heating, stirring are concentrated into and become paste, and the first extract is made as a result,.

It prepares the second extract step: component B is transferred in round-bottomed flask, according to solid-liquid ratio (i.e. the ratio between quality volume Value) it is 1:15, the ethyl alcohol that mass fraction is 75% is added into round-bottomed flask, and stirring rod is set and is stirred in a round bottom flask 20min (or round-bottomed flask is placed on shaking table between oscillating phase is seasonable).Round-bottomed flask is connected into the pipeline system of condensing reflux Standby to be placed in round-bottomed flask in the thermostat water bath that temperature is 60 DEG C at a set of refluxing extraction device, rotation round-bottomed flask carries out Refluxing extraction 5h.After refluxing extraction process, mixed liquor is filtered by double gauze, filters out the dregs of a decoction.Filtrate is collected, will be filtered Liquid is transferred in vacuum rotary evaporator, and rotary evaporation is to extracting solution under the conditions of vacuum degree is -0.12MPa and temperature is 50 DEG C There is wall built-up (to start to occur sticky) to stop when phenomenon, concentrate is made.Into concentrate obtained, the hydrochloric acid of addition 0.5% is molten The ratio between volume of liquid, concentrate and hydrochloric acid solution is 1:5, and the stirring of Bian Tianjia hydrochloric acid solution side stands 1h, obtains acid dissolution part With non-acid dissolution part, taking acid dissolution part pH value is 12 ammonium hydroxide tune pH10.4, continuously adds methylene chloride, wherein acid The volume ratio of dissolution part and methylene chloride is 1:5, oscillation mixing, stratification.It draws dichloromethane layer and is transferred to vacuum Rotary Evaporators, it is 42 DEG C that evaporating temperature, which is arranged, and vacuum degree is -0.01MPa, is evaporated under reduced pressure into powdered, obtained second extraction Object.

Preparation third extract step: component C is transferred in round-bottomed flask, according to solid-liquid ratio (i.e. the ratio between quality volume Value) be 1:2 amount add 2mol/L acetum into round-bottomed flask, be according still further to solid-liquid ratio (i.e. the ratio between quality volume value) The amount of 1:15 adds dehydrated alcohol, and oscillation mixes.Round-bottomed flask is transferred in Ultrasound Instrument, adjusting ultrasonic power is 100W, is surpassed Sound extracts 60min.After ultrasonic extraction process, extracting solution is filtered by double gauze, the dregs of a decoction is filtered out, collects filtrate.It will Filtrate is transferred in centrifuge tube, and centrifuge tube, which is placed in a centrifuge, to be centrifuged, centrifuge RPMs 8000r/min, and centrifugation time is 3min.Centrifuge tube is taken out after centrifugation, absorption supernatant liquor, which is transferred in vacuum rotary evaporator, is condensed into medicinal extract shape, In, thickening temperature is 60 DEG C, and vacuum degree is -0.15MPa.The chloroform for adding 5 times of supernatant liquor volumes into concentrate bottle again, will be dense Contracting bottle is placed in the thermostat water bath that temperature is 65 DEG C, and spin concentration bottle carries out refluxing extraction 55min.After refluxing extraction, Extracting solution is transferred in triangular flask and triangular flask is placed on the thermostat water bath that temperature is 70 DEG C, in heating evaporation chloroform Third extract is made until chloroform evaporated is complete and is concentrated into paste (or blocky) in stirring.

Preparations shaping step: the first extract, the second extract and third extract are stirred and evenly mixed in advance, primary is made Medicinal extract, then primary extract, menthol and hydrogel are uniformly mixed, it is modulated into emulsifiable paste shape with glycerol, obtains improvement of the present invention Eye is dry, eye is puckery, asthenopia cold compress gel.By above-mentioned improvement eye is dry, eye is puckery, asthenopia cold compress gel is spread evenly across 50 On non-woven fabrics, release paper is covered, obtained present invention improvement eye is dry, eye is puckery, asthenopia cold compress eye sticker.Wherein, primary extract, thin The weight ratio of lotus brain and hydrogel is 10:10:10.

Comparative example 1

Material preparation step: weigh the Rhizoma Et Radix Notopterygii dried be 7g, tripterygium wilfordii 6g, cordate houttuynia 13g, honeysuckle 9g, Radix Rehmanniae 5g and anti- Wind 5g constituent component A.Weigh the balsam pear 7g dried, imperial tongue 9g, aloe 7g and coptis root 12g constituent component B.Weigh the knife dried Beans 9g, fennel seeds 12g, polygonum cuspidate 16g and folium eucalypti 11g constituent component C.

It prepares extract step: component A, B and C is transferred in casserole medicinal cupping, add 3000ml into the casserole medicinal cupping Pure water (is also required to guarantee water by addition pure water in decoction process), is stirred to be placed on mild fire and decocts 2h, Casserole medicinal cupping is stirred continuously or vibrated in decoction process.After decoction, natural cooling, by decoction liquor double gauze mistake Filter, filtrate be transferred to Rotary Evaporators be concentrated under reduced pressure into it is thick.Wherein, the temperature of reduced pressure is 82 DEG C, is depressurized dense The vacuum degree of contracting is -0.07MPa.

Preparations shaping step: extract, menthol and hydrogel are uniformly mixed, and are modulated into emulsifiable paste shape with glycerol, that is, are made Improvements eye of the invention is done, eye is puckery, cold compress gel of asthenopia.By above-mentioned improvement eye is dry, eye is puckery, asthenopia cold compress gel is equal It is even to be coated on 50 non-woven fabrics, release paper is covered, obtained present invention improvement eye is dry, eye is puckery, asthenopia cold compress eye sticker.Its In, the weight ratio of primary extract, menthol and hydrogel is 10:3:20.

Comparative example 2

Material preparation step: weigh the Rhizoma Et Radix Notopterygii dried be 7g, tripterygium wilfordii 6g, cordate houttuynia 13g, honeysuckle 9g, Radix Rehmanniae 5g and anti- Wind 5g constituent component A.Weigh the sword bean 9g dried, fennel seeds 12g, polygonum cuspidate 16g and folium eucalypti 11g constituent component C.

It prepares the first extract step: component A is transferred in round-bottomed flask, according to solid-liquid ratio (i.e. the ratio between quality volume Value) it is 1:10, the ethyl alcohol that mass fraction is 80% is added into round-bottomed flask, and stirring rod is set and is stirred in a round bottom flask 50min (or round-bottomed flask is placed on shaking table between oscillating phase is seasonable).Round-bottomed flask is connected into the pipeline system of condensing reflux Standby that round-bottomed flask is placed in 78 DEG C of thermostat water baths at a set of refluxing extraction device, rotation round-bottomed flask carries out refluxing extraction 1.5h.After refluxing extraction process, mixed liquor is filtered by double gauze, filters out the dregs of a decoction.Filtrate is collected, filtrate is shifted Into vacuum rotary evaporator, rotary evaporation to extracting solution is hung under the conditions of vacuum degree is -0.04MPa and temperature is 68 DEG C Wall (starts to occur sticky) to stop when phenomenon, then dehydrated alcohol is added into round-bottomed flask, and the addition volume of dehydrated alcohol is dense 5 times of contracting liquid product vibrate round-bottomed flask and concentrate are uniformly mixed with dehydrated alcohol, stand for 24 hours, be layered, nothing The contamination precipitations such as protein, polysaccharide, inorganic salts are precipitated in water-ethanol, the effective component being achieved in purified components A.In absorption Clear liquid is simultaneously transferred on 70 DEG C of thermostat water baths, and heating, stirring are concentrated into and become paste, and the first extract is made as a result,.

Preparation third extract step: component C is transferred in round-bottomed flask, according to solid-liquid ratio (i.e. the ratio between quality volume Value) be 1:3 amount add 1mol/L acetum into round-bottomed flask, be according still further to solid-liquid ratio (i.e. the ratio between quality volume value) The amount of 1:10 adds dehydrated alcohol, and oscillation mixes.Round-bottomed flask is transferred in Ultrasound Instrument, adjusting ultrasonic power is 300W, is surpassed Sound extracts 35min.After ultrasonic extraction process, extracting solution is filtered by double gauze, the dregs of a decoction is filtered out, collects filtrate.It will Filtrate is transferred in centrifuge tube, and centrifuge tube, which is placed in a centrifuge, to be centrifuged, centrifuge RPMs 6000r/min, and centrifugation time is 8min.Centrifuge tube is taken out after centrifugation, absorption supernatant liquor, which is transferred in vacuum rotary evaporator, is condensed into medicinal extract shape, In, thickening temperature is 60 DEG C, and vacuum degree is -0.15MPa.The chloroform of 3.5 times of supernatant liquor volumes is added into concentrate bottle again, it will Concentrate bottle is placed in the thermostat water bath that temperature is 55 DEG C, and spin concentration bottle carries out refluxing extraction 60min.Refluxing extraction terminates Afterwards, extracting solution is transferred in triangular flask and triangular flask is placed on the thermostat water bath that temperature is 60 DEG C, side heating evaporation chlorine Third extract is made until chloroform evaporated is complete and is concentrated into paste (or blocky) in imitative side stirring.

Preparations shaping step: the first extract and third extract are stirred and evenly mixed into obtained primary extract in advance, then will be first Grade medicinal extract, menthol and hydrogel are uniformly mixed, and are modulated into emulsifiable paste shape with glycerol, and obtaining the present invention improves that eye is dry, eye is puckery, eye The cold compress gel of fatigue.By above-mentioned improvement eye is dry, eye is puckery, asthenopia cold compress gel is spread evenly across on 50 non-woven fabrics, lid Upper release paper, obtained present invention improvement eye is dry, eye is puckery, asthenopia cold compress eye sticker.Wherein, primary extract, menthol and water-setting The weight ratio of glue is 10:3:20.

Effect example one: effect assessment

Clinical data: choose in August, 2011 in June, 2017 store be diagnosed as xerophthalmia, asthenope 400 (786 eyes), including male patient 180 and female patient 220.Age is only 8 years old the smallest, oldest to reach 82 Year, average age are 45 years old.The course of disease is that 3~30 days patients account for 266, and the course of disease is that 31~180 days patients account for 115, disease Journey is more than that 180 days patients account for 19.Above-mentioned patient diagnosed is divided into 8 groups, respectively test group 1~5, it is control group 1, right According to group 2 and positive controls, every group patient 50.Above-mentioned patients with dry eye clinical manifestation is the dry adacrya of conscious eye, photophobia, conjunctiva Hyperemia, secretion is a little, angle xeroma, matt, and corneal epithelium mist-like bleary, fluorescent staining colours extensively, Schirmer tests 5mm hereinafter, eyesight 0.3 is hereinafter, severe one angle xeroma angling, atretoblepharia, ulcer of the cornea cause blindness. Asthenope's Symptoms are that reading time cannot be lasting, are read for a long time with headache, sleepy, diplopia, blurred vision, Eyes have ametropia situation more.

Treatment method: 50 patients in test group 1 smeared around eyelet daily improvement eye prepared by embodiment 1 it is dry, Puckery, asthenopia the cold compress eye sticker of eye each one is pasted, each application time 1h or more daily morning and evening.50 patients in test group 2 are every The improvement eye that day smears the preparation of embodiment 2 around eyelet is dry, eye is puckery, asthenopia cold compress eye sticker, daily morning and evening each patch, often Secondary application time 1h or more.50 patients in test group 3 smeared around eyelet daily improvement eye prepared by embodiment 3 it is dry, Puckery, asthenopia the cold compress eye sticker of eye each one is pasted, each application time 1h or more daily morning and evening.50 patients in test group 4 are every The improvement eye that day smears the preparation of embodiment 4 around eyelet is dry, eye is puckery, asthenopia cold compress eye sticker, daily morning and evening each patch, often Secondary application time 1h or more.50 patients in test group 5 smeared around eyelet daily improvement eye prepared by embodiment 5 it is dry, Puckery, asthenopia the cold compress eye sticker of eye each one is pasted, each application time 1h or more daily morning and evening.50 patients in control group 1 are every The improvement eye that day smears the preparation of comparative example 1 around eyelet is dry, eye is puckery, asthenopia cold compress eye sticker, daily morning and evening each patch, often Secondary application time 1h or more.50 patients in control group 2 smeared around eyelet daily improvement eye prepared by comparative example 2 it is dry, Puckery, asthenopia the cold compress eye sticker of eye each one is pasted, each application time 1h or more daily morning and evening.50 trouble in positive controls Person uses hydroxychloroquine sulfate piece to treat daily, and patient 30min after breakfast and dinner takes oral way, every time 200mg, 2 times a day.Above-mentioned treatment continues one month.

Therapeutic effect criterion.Effective: related symptoms, the sign of patient is significantly improved, by checking that display is every Observation index has significant improvement.Effective: related symptoms, the sign of patient has a degree of improvement, is shown by coherence check Every observation index has a degree of improvement.Invalid: its related symptoms, sign do not have any significant change.Total effective rate= (effective number of cases+effective number of cases)/total number of cases.Situation after counting each group patient treatment, statistical result are shown in Table 1.

Table 1

By above-mentioned 1 result of table it is found that compared to control group 1 and control group 2, the improvement eye of embodiment 1-5 preparation is dry, eye Puckery, asthenopia cold compress eye sticker has significant curative effect for xerophthalmia, asthenope, and total effective rate is promoted to 88% or more, Especially the improvements eye for preparing of embodiment 3 is dry, eye is puckery, asthenopia cold compress eye sticker, for xerophthalmia, asthenopia it is total effective Rate reaches 96%, greatly exceeds the total effective rate of positive controls (hydroxychloroquine sulfate piece), can be applied to clinical treatment With prevention dry eyes, asthenopia symptom.

Effect example two: the pretherapy and post-treatment eye of above-mentioned 400 patients is done, eye is puckery and asthenopia scores

The dry standards of grading of eye: 0 point, anophthalmia does symptom；1 point, occasionally there is xerophthalmia shape, it is unobvious；It 2 points, needs by artificial Tear is soaked, and xerophthalmia shape is obvious；3 points, xerophthalmia turns serious, has sand milling to feel.The puckery standards of grading of eye: 0 point, the puckery disease of anophthalmia Shape；1 point, occasionally there is the puckery symptom of eye, it is unobvious；2 points, the puckery symptom of eye is obvious, keeps in dark place, easily sheds tears；3 points, the puckery symptom of eye is obvious, eyeball It is covered with the trace of blood, eyeball is red and swollen, and secretion is more.Asthenopia standards of grading: the company of being both required to before and after above-mentioned 400 treatment patients It is continuous to read novel 1h, the asthenopia situation of patient is consulted after reading.Degree of fatigue includes: 0 point, and non fatigue feeling, energy fills It is abundant；1 point, slightly feeling of fatigue need to rub one's eyes once in a while；2 points, asthenopia sense is obvious, and continuous several times is needed to rub one's eyes or suspend It reads；3 points, eye is very tired, continuously sheds tears in long-time reading process, and Ocular irritation sense is obvious, seriously affects patient and reads It reads.Statistical result is shown in Table 2-4

Table 2

* the dry scoring of eye before being expressed as compared to treatment, the dry scoring of eye after treatment decline significant (P < 0.05)；* is indicated For compared to the dry scoring of eye before treatment, the dry scoring of eye after treatment declines extremely significant (P < 0.01).

From the above results, the improvements eye for receiving embodiment 1-5 preparation is dry, eye is puckery, asthenopia cold compress eye sticker is treated Afterwards, the dry scoring decline of patient is extremely significant, shows that present invention improvement eye is dry, eye is puckery, asthenopia cold compress eye sticker is for xerophthalmia Shape has significant curative effect, can be used for clinical treatment or prevention and treatment eye is done, eye is puckery, asthenopia disease.With positive controls phase Compare, the improvements eye for receiving embodiment 1-5 preparation is dry, eye is puckery, after the treatment of asthenopia cold compress eye sticker, and patient's eye is dry to score more It is low, there is better curative effect.It is on the other side to be, after the treatment patients in control group 1, control group 2, the dry scoring of patient's eye Decline is not significant, does not have statistical significance.Show that improvement eye prepared by comparative example 1 and comparative example 2 is dry, eye is puckery, asthenopia Cold compress eye sticker does not have significant curative effect for xerophthalmia shape, is compared with test group 1-5, the dry scoring decline of patient's eye It is low, effect is poor.

Table 3

* be expressed as compared to treatment before the puckery scoring of eye, the puckery scoring of eye after treatment declines extremely significant (P < 0.01).

From the above results, the improvements eye for receiving embodiment 1-5 preparation is dry, eye is puckery, asthenopia cold compress eye sticker is treated Afterwards, the puckery scoring decline of patient's eye is extremely significant, shows that present invention improvement eye is dry, eye is puckery, asthenopia cold compress eye sticker disease puckery for eye Shape has significant curative effect, can be used for clinical treatment or prevention and treatment eye is done, eye is puckery, asthenopia disease.With positive controls phase Compare, the improvements eye for receiving embodiment 1-5 preparation is dry, eye is puckery, after the treatment of asthenopia cold compress eye sticker, and patient's eye is puckery to score more It is low, there is better curative effect.It is on the other side to be, after the treatment patients in control group 1, control group 2, the puckery scoring of patient's eye Decline is not significant, does not have statistical significance.Show that improvement eye prepared by comparative example 1 and comparative example 2 is dry, eye is puckery, asthenopia Cold compress eye sticker symptom puckery for eye does not have significant curative effect, is compared with test group 1-5, the puckery scoring decline of patient's eye It is low, effect is poor.

Table 4

* the asthenopia scoring before being expressed as compared to treatment, the asthenopia scoring after treatment decline significant (P < 0.05)；** Asthenopia scoring before being expressed as compared to treatment, the asthenopia scoring after treatment decline extremely significant (P < 0.01).

From the above results, the improvements eye for receiving embodiment 1-5 preparation is dry, eye is puckery, asthenopia cold compress eye sticker is treated Afterwards, patient's asthenopia scoring decline is significant, shows that present invention improvement eye is dry, eye is puckery, asthenopia cold compress eye sticker is for asthenopia Symptom has significant curative effect, can be used for clinical treatment or prevention and treatment eye is done, eye is puckery, asthenopia disease.With positive controls It compares, the improvements eye for receiving embodiment 1-5 preparation is dry, eye is puckery, after the treatment of asthenopia cold compress eye sticker, and patient's asthenopia scores It is lower, there is better curative effect.It is on the other side to be, after the treatment patients in positive controls, the scoring of patient's asthenopia Decline is not significant, does not have statistical significance；After treatment patients in control group 1, control group 2, under the puckery scoring of patient's eye Drop is not significant, does not have statistical significance.Show that improvement eye prepared by comparative example 1, comparative example 2 is dry, eye is puckery, asthenopia cold compress Eye sticker and positive controls do not have significant curative effect for asthenopia symptom, are compared with test group 1-5, patient's eye The lower reduction of fatigue scoring, effect are poor.

Effect example three: recurrence statistics

After above-mentioned 400 patients terminate treatment, the complete healing patient 10 in test group 1-5 and positive controls is chosen Example carries out follow-up investigation, i.e., the patient that eye is dry, eye is puckery, asthenopia symptom completely disappears.Whether go out again in follow-up investigation half a year Lose face symptoms and the recurrence rates such as dry, eye is puckery, asthenopia, recurrence rate=(eye does the puckery number of cases of number of cases+eye+asthenopia number of cases)/total Number of cases, statistical result are shown in Table 5.

Table 5

From the above results, it is higher to go out symptoms recurrence probabilities such as dry, eye is puckery, asthenopia of losing face by positive controls patient, Reach 50%.In comparison, after the improvements eye for receiving embodiment 1-5 preparation is dry, eye is puckery, asthenopia cold compress eye sticker is treated, It is lower that patient goes out symptoms recurrence probabilities such as dry, eye is puckery, asthenopia of losing face, be particularly suited for clinical treatment or prevention and treatment eye it is dry, Puckery, the asthenopia disease of eye.

The above embodiment is only the preferred embodiment of the present invention, and the scope of protection of the present invention is not limited thereto, The variation and replacement for any unsubstantiality that those skilled in the art is done on the basis of the present invention belong to institute of the present invention Claimed range.

Claims (10)

1. a kind of improvement eye is dry, eye is puckery, asthenopia cold compress eye sticker, which is characterized in that including activity extract, menthol and water Gel；

The activity extract be Rhizoma Et Radix Notopterygii, tripterygium wilfordii, cordate houttuynia, honeysuckle, Radix Rehmanniae, radix saposhnikoviae, balsam pear, imperial tongue, aloe, coptis root, Sword bean, fennel seeds, polygonum cuspidate and folium eucalypti extract；

The weight ratio of the activity extract, menthol and hydrogel is 10:1~10:10~50.

2. improvement eye according to claim 1 is dry, eye is puckery, asthenopia cold compress eye sticker, which is characterized in that by weight Meter, the Rhizoma Et Radix Notopterygii be 3~15 parts, the tripterygium wilfordii be 2~10 parts, the cordate houttuynia be 6~20 parts, the honeysuckle be 4~ 16 parts, the Radix Rehmanniae is 1~10 part, and the radix saposhnikoviae is 2~8 parts, and the balsam pear is 3~12 parts, and the dragon tongue is 5~12 parts, The aloe is 3~10 parts, and the coptis root is 5~20 parts, and the sword bean is 3~15 parts, and the fennel seeds are 4~18 parts, institute Stating polygonum cuspidate is 6~24 parts, and the folium eucalypti is 6~15 parts.

3. improvement eye according to claim 2 is dry, eye is puckery, asthenopia cold compress eye sticker, which is characterized in that by weight Meter, the Rhizoma Et Radix Notopterygii be 5~10 parts, the tripterygium wilfordii be 4~8 parts, the cordate houttuynia be 10~15 parts, the honeysuckle be 8~ 12 parts, the Radix Rehmanniae is 3~7 parts, and the radix saposhnikoviae is 4~6 parts, and the balsam pear is 5~9 parts, and the dragon tongue is 7~10 parts, institute Stating aloe is 5~9 parts, and the coptis root is 8~16 parts, and the sword bean is 6~12 parts, and the fennel seeds are 9~15 parts, the tiger Cane is 12~20 parts, and the folium eucalypti is 9~12 parts.

4. improvement eye according to claim 3 is dry, eye is puckery, asthenopia cold compress eye sticker, which is characterized in that by weight Meter, the Rhizoma Et Radix Notopterygii are 7 parts, and the tripterygium wilfordii is 6 parts, and the cordate houttuynia is 13 parts, and the honeysuckle is 9 parts, and the Radix Rehmanniae is 5 Part, the radix saposhnikoviae is 5 parts, and the balsam pear is 7 parts, and the dragon tongue is 9 parts, and the aloe is 7 parts, and the coptis root is 12 parts, institute Stating sword bean is 9 parts, and the fennel seeds are 12 parts, and the polygonum cuspidate is 16 parts, and the folium eucalypti is 11 parts.

5. improvement eye according to claim 1-4 is done, the preparation method of puckery, asthenopia the cold compress eye sticker of eye, It is characterized in that, comprising the following steps:

Stock: weighing Rhizoma Et Radix Notopterygii, tripterygium wilfordii, cordate houttuynia, honeysuckle, Radix Rehmanniae and windproof constituent component A, weighs balsam pear, imperial tongue, aloe And coptis root constituent component B, sword bean, fennel seeds, polygonum cuspidate and folium eucalypti constituent component C are weighed, component A, B and C are pulverized, crosses 10 ~40 meshes；

It prepares the first extract: 75~95% ethyl alcohol is added with the solid-liquid ratios of 1:5~15 in component A, stirring, impregnate 20~ After 80min, concentrate is concentrated under reduced pressure into 0.5~2.5h of refluxing extraction at 70~85 DEG C, filtering, filtrate, adds anhydrous second Alcohol standing, Precipitation take supernatant heating to be concentrated into paste, the first extract are made；

It prepares the second extract: 75~95% ethyl alcohol is added with the solid-liquid ratios of 1:4~10 in component B, stirring, impregnate 20~ After 60min, 1~5h of refluxing extraction at 60~70 DEG C, filtering, filtrate are concentrated under reduced pressure to give concentrate, concentrate with 0.5~ 5% dissolving with hydrochloric acid takes lysate part to be adjusted to pH8.6~10.4 with ammonium hydroxide, continuously adds the two of 2~5 times of lysate volume Dichloromethane layer is concentrated under reduced pressure into powdered, obtained second extract for chloromethanes, mixing, layering；

Prepare third extract: by component C with the acetum of 0.5~2mol/L of solid-liquid ratio addition of 1:2~5 and with 1:5 Dehydrated alcohol is added in~15 solid-liquid ratio, and 100~500W ultrasonic extraction time is 10~60min, 3000~8000r/min centrifugation 3~15min, clear liquid is condensed into medicinal extract shape after centrifugation, and medicinal extract chloroform 20~90min of refluxing extraction of 2~5 times of volumes is mentioned It takes liquid in being concentrated into paste on water-bath, third extract is made；

Preparations shaping: the first extract, the second extract, third extract, menthol and hydrogel are uniformly mixed, glycerol is used It is modulated into emulsifiable paste shape, being made improves that eye is dry, eye is puckery, asthenopia cold compress eye sticker.

6. improvement eye according to claim 5 is done, the preparation method of puckery, asthenopia the cold compress eye sticker of eye, which is characterized in that In preparing the first extract step, 80% ethyl alcohol is added with the solid-liquid ratio of 1:10 in component A, after stirring, impregnating 50min, 78 Refluxing extraction 1.5h at DEG C, double gauze filtering, filtrate, which are transferred in vacuum rotary evaporator, to be concentrated under reduced pressure into concentrate, concentration Temperature is 68 DEG C, and vacuum degree is -0.04MPa；

Be added 3 times of volumes of concentrate dehydrated alcohol stand, layering, take supernatant be placed on 70 DEG C of thermostat water baths be concentrated into it is thick The first extract is made in paste.

7. improvement eye according to claim 5 is done, the preparation method of puckery, asthenopia the cold compress eye sticker of eye, which is characterized in that In preparing the second extract step, 85% ethyl alcohol is added with the solid-liquid ratio of 1:8 in component B, after stirring, impregnating 30min, 65 DEG C Lower refluxing extraction 2.5h, filtering, filtrate are transferred in vacuum rotary evaporator and are concentrated under reduced pressure to give concentrate, wherein concentration temperature Degree is 55 DEG C, and vacuum degree is -0.08MPa.

8. improvement eye according to claim 7 is done, the preparation method of puckery, asthenopia the cold compress eye sticker of eye, which is characterized in that 1.5% dissolving with hydrochloric acid of concentrate, separates lysate part, takes lysate part to be adjusted to pH9.4 with ammonium hydroxide, continuously add molten Dichloromethane layer is concentrated under reduced pressure into powdered, obtained second extract for the methylene chloride that 4 times of product of solution liquid, mixing, layering；

Wherein, it is 37 DEG C that temperature, which is concentrated under reduced pressure, and vacuum degree is -0.02MPa.

9. improvement eye according to claim 5 is done, the preparation method of puckery, asthenopia the cold compress eye sticker of eye, which is characterized in that In preparation third extract step, by component C with the acetum of the solid-liquid ratio addition 1mol/L of 1:3 and with the material of 1:10 Dehydrated alcohol is added in liquor ratio, and 300W ultrasonic extraction 35min, 6000r/min are centrifuged 8min, and clear liquid is transferred to vacuum rotating after centrifugation Medicinal extract shape is condensed into evaporimeter, wherein thickening temperature is 60 DEG C, and vacuum degree is -0.15MPa；

By the medicinal extract chloroform refluxing extraction 60min of 3.5 times of volumes, extracting solution is in being concentrated into thick paste on 45 DEG C of thermostat water baths Shape.

10. improvement eye according to claim 5 is done, the preparation method of puckery, asthenopia the cold compress eye sticker of eye, feature exists In, in preparations shaping step, the first extract, the second extract, third extract is mixed in advance, primary extract is made, then Menthol and hydrogel is added, is uniformly mixed；

Wherein, the weight ratio of primary extract, menthol and hydrogel is 10:3:20.