CN109735466A - A kind of promotion low temperature, microorganism formulation of low-oxygen environment plant growth and preparation method thereof - Google Patents
A kind of promotion low temperature, microorganism formulation of low-oxygen environment plant growth and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to plant growth microorganism formulation technical fields, and in particular to a kind of promotion low temperature, microorganism formulation of low-oxygen environment plant growth and preparation method thereof.It is described promote low temperature, low-oxygen environment plant growth microorganism formulation mainly include starch, cold-resistant lower oxygen concentration resistance microbial bacterial agent, algal polysaccharides, according to parts by weight, 60-70 parts of starch, 8-10 parts of cold-resistant lower oxygen concentration resistance microbial bacterial agent, 20-32 parts of algal polysaccharides.Additionally provide the promotion low temperature, low-oxygen environment plant growth microorganism formulation preparation method.The microorganism formulation of promotion low temperature, low-oxygen environment plant growth of the invention is individually or agroforestry organic fertilizer etc. can be effectively improved plant rhizosphere ecological environment under low temperature, low-oxygen environment, remarkably promotes plant growth.
Description
Technical field
The invention belongs to plant growth microorganism formulation technical fields, and in particular to a kind of promotion low temperature, low-oxygen environment are planted
The microorganism formulation and preparation method thereof of object growth.
Background technique
Low temperature stress is to influence one of plant growth, development and important environmental restrictions factor of geographical distribution.Low temperature stress
Influence to plant is mainly reflected in enzymatic activity, membranous system, cell dehydration etc., leads to cell metabolism disorder, and even cell is dead
It dies.Plant by low temperature stress is short and small, and the growth rate of blade reduces, growth cycle extends, Contents of Photosynthetic Pigments reduces
The decline of (since chlorophyll is destroyed), photosynthetic rate, content of organics are low；The reduction of root zone temperature, reduces root activity, drop
Low absorption of the root to mineral element；Low temperature also results in the reduction of plant organic matter content.When temperature lower than 10 DEG C will result in it is cold
Evil, then causes freeze injury lower than 0 DEG C, temperature be below the freezing point it is following will make occur ice crystal in plant tissue, the intracorporal original of cell
Raw plastid is made plant come to harm by irreversible destruction.
It damages to plants caused by sudden drop in temperature, is the injury that 0 DEG C or more low temperature generates it in vegetation season, usually also known as chilling injury.It damages to plants caused by sudden drop in temperature
Make plant physiology activity by obstacle, certain tissues are destroyed when serious.It but is at 0 DEG C of temperature or more, sometimes due to damaging to plants caused by sudden drop in temperature
Even occur under conditions of close to 20 DEG C, after crop damage, appearance has the title of " mute's calamity " without significant change.It is cold
Influence of the evil to plant physiology is mainly manifested in: 1. weakening photosynthesis.As various crops with 24 DEG C when photosynthesis it is strong
Degree is 100%, then the intensity of photosynthesis of soybean is 85% under the conditions of 12 DEG C, rice 81%, sorghum 74%, corn
It is 62%.Low temperature makes intensity of photosynthesis reduce by 15~38%.2. reducing Nutrient Absorption.Low temperature reduces absorption of the root system to nutrient
Ability is absorbed as 50% to ammonium nitrogen in 12 DEG C of Rice under Condition, phosphorus to be absorbed as 100% to nutrient under the conditions of 24 DEG C
It is 44%, potassium 42%；Soybean is absorbed as 87% to ammonium nitrogen, phosphorus 55%, potassium 70%, significant reduction.3. influencing
The operating of nutrient.Low temperature can interfere photosynthate and mineral matter nutritional to growth conveyed, the organ for being growing crop
Slight of stature, degeneration or dead due to nutrient deficiency.The phase is extended in young fringe, low temperature makes stalk be obstructed to the conveying of the nutrient of fringe portion, anther
Tissue cannot normally convey carbohydrate to pollen, to interfere enriching and the normal crack of anther, loose powder for pollen.It is filling
During slurry, low temperature not only reduces the synthesis of carbohydrate because weakening photosynthesis, but also hinders photosynthate to fringe
The transporting in portion.
Endogenous rhythm, physiological status of the plant cold resistance by external environmental condition (special soil regime) and development of plants
Etc. seriously affecting.Guarantee that soil kilter is the suitable condition that plant cold resistance gives full expression to, at low temperature to raising plant
It well grows significant.
Hypoxia Stress causes very big influence to the growth of plant, and Hypoxia Stress is as the main non-life of higher plant
Object Stress Factors generality with higher is not easy being noticeable property and serious harmfulness, to plant (including China's grain produce
Amount) influence it is huge.Plant Hypoxia Stress influences to specifically include that the change of Respiratory Metabolism Pathway, reproducibility caused by plant
Harmful toxic matter accumulation, mineral matter element, which absorbs unbalance, moisture absorption, to be reduced, hormone metabolism disorder etc..In agricultural production frequently with
Following methods carry out the prevention and treatment to Hypoxia Stress: enhancing field management, exclude ponding in time；The rational application of fertilizer, regulating crop growth；?
Will be in conjunction with local climate condition and irrigation conditions before sowing, selection selection suitable crop and varieties of plant.
People have also researched and developed the special preparation or fertilizer of many raising freezing tolerance of plant or anti-hypoxemia ability, but this
Usually to plant, with very limited specific aim, perhaps raw material difficulty is taken or application range is narrow for a little preparations or fertilizer, cannot
It is extensive to carry out production application, and application effect is general；It cannot especially be coerced simultaneously for low temperature and low-oxygen environment.
Summary of the invention
Aiming at the problems existing in the prior art, the present invention provide it is a kind of promotion low temperature, low-oxygen environment plant growth it is micro-
Biological agent and preparation method thereof, specific technical solution are as follows:
A kind of microorganism formulation promoting low temperature, low-oxygen environment plant growth, including starch, cold-resistant lower oxygen concentration resistance microbial bacteria
Agent and algal polysaccharides, according to parts by weight, 60-70 parts of starch, 8-10 parts of cold-resistant lower oxygen concentration resistance microbial bacterial agent, algal polysaccharides 20-
32 parts.
The cold-resistant lower oxygen concentration resistance microorganism fungus kind is the brevibacterium Brevibacterium sp.TS22 derived from Qinghai-Tibet Platean,
It is obtained by low altitude area and room temperature domestication, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, protects
Hiding number are as follows: CGMCC 16576, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation time 2018.10.12.
The acclimation conditions of the low altitude area and room temperature are as follows: train the TS22 strain inoculated for being isolated from Qinghai-Tibetan plateau soil to LB
It supports in base, in secondary culture, LB culture medium prescription are as follows repeatedly under room temperature: tryptone 10g/L/ yeast extract 5g/
L, sodium chloride 10g/L adjusts pH to 7.4,121 DEG C of high pressure steam sterilization 20min with NaOH.The identified bacterial strain, which has, produces IAA,
The plant growth-promotings attribute such as siderophore.
In use, taming again for condition corresponding with area's main environmental conditions will be used, then acclimation conditions must be passed through are as follows:
The TS22 bacterial strain that will be tamed by low altitude area and room temperature, is inoculated into LB culture medium, the passage training repeatedly under the conditions of 10-20 DEG C
Support, LB culture medium prescription is as follows: tryptone 10g/L, yeast extract 5g/L, sodium chloride 10g/L, with NaOH adjust pH to
7.4,121 DEG C of high pressure steam sterilization 20min.
The cold-resistant lower oxygen concentration resistance microorganism fungus kind further includes phosphate solubilizing microorganism, and the phosphate solubilizing microorganism is huge gemma bar
Bacterium Bacillus megaterium (CICC20665 is purchased from Chinese industrial Microbiological Culture Collection administrative center), pseudomonad
Pseudomonas sp. (CGMCC10242, be purchased from China Committee for Culture Collection of Microorganisms's common micro-organisms center) or
One in agrobacterium Agrobacterium sp. (CICC10214 is purchased from Chinese industrial Microbiological Culture Collection administrative center)
Kind is a variety of；
In use, need to domestication Jing Guo condition corresponding with area's main environmental conditions will be used, acclimation conditions are as follows: will solve
Phosphorus bacterial strain is inoculated into Phos culture respectively and is based on carrying out strain passage culture, Phos culture medium prescription used at 10-20 DEG C
Are as follows: glucose 10g/L, (NH4)2SO40.5g/L, NaCl 3g/L, MgSO4·7H2O 0.5g/L, KCl 0.5g/L, FeSO4·
7H2O 0.03 g/L, MnSO40.03g/L, Ca3(PO4)27.2,115 DEG C of sterilizing 20min of 5g/L, pH.
The weight of the phosphate solubilizing microorganism accounts for the 0-20% of cold-resistant lower oxygen concentration resistance microbial bacterial agent.
It is a kind of promote low temperature, low-oxygen environment plant growth microorganism formulation preparation method, specific steps include: that will form sediment
Powder, cold-resistant lower oxygen concentration resistance microbial bacterial agent and algal polysaccharides are put into blender and are mixed, and the revolving speed of blender is set as 100-
300r/min, temperature are room temperature, and the microorganism for promoting low temperature, low-oxygen environment plant growth is made in reaction time 10-30min
Preparation.
A kind of microorganism formulation promoting low temperature, low-oxygen environment plant growth of the present invention is for promoting low temperature, low
The purposes of oxygen environment plant growth, the microorganism formulation exclusive use or compound for promoting low temperature, low-oxygen environment plant growth
Agricultural organic fertilizer uses, in agroforestry organic fertilizer in use, the microorganism system for promoting low temperature, low-oxygen environment plant growth
The mass ratio of agent and agricultural organic fertilizer is 2-4:98-96.
The Brevibacterium sp.TS22 sequence information are as follows:
CCCCGCCATCGGCTTCGTTCGAATGCCATGCATAGCGACTCGCACGCCAAGCGTGCGTCCCTGAGCCA
TGATC AAACTCTATGAGTAACACGTGGGCAACCTGCCTATAAGACTGGGATAACTTCGGGAAACCGGAGCTAATA
CCG GATACGTTCTTTTCTCGCATGAGAGAAGATGGAAAGACGGTTTACGCTGTCACTTATAGATGGGCCCGCGGCG
CATTAGCTAGTTGGTGAGGTAATGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCA CAC
TGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAG TCTGACG
GAGCAACGCCGCGTGAACGAAGAAGGCCTTCGGGTCGTAAAGTTCTGTTGTTAGGGAAGAACAA GTACCAGAGTA
ACTGCTGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCG CGGTAATACGTAGG
TGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTCCTTAAGTC TGATGTGAAAGCCCACG
GCTCAACCGTGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAA AGTGGAATTCCAAAGTGTAGCG
GTGAAATGCGTAGAGATTTGGAGGAACACCAGTGGCGAAGGCGACTTTC TGGTCTGTAACTGACACTGAGGCGCG
AAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCC GTAAACGATGAGTGCTAAGTGTTAGAGGG
TTTCCGCCCTTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCC TGGGGAGTACGGCCGCAAGGCTGAAACTCAA
AGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTG GTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGT
CTTGACATCCTCTGACAACCCTAGAGATAGGGCTTTC CCCTTCGGGGGACAGAGTGACAGGTGGTGCATGGTTGT
CGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTC CCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCA
GTTGGGCACTCTAAGGTGACTGCCGGTGACA AACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTA
TGACCTGGGCTACACACGTGCTACAATG GATGGTACAAAGGGCTGCAAACCTGCGAAGGTAAGCGAATCCCATAA
AGCCATTCTCAGTTCGGATTGCAGG CTGCAACTCGCCTGCATGAAGCCGGAATCGCTAGTAATCGCGGATCAGCA
TGCCGCGGTGAATACGTTCCCGG GCCTTGTACACACCGCC。
The invention has the benefit that
The core component of microorganism formulation of the present invention for promoting low temperature, low-oxygen environment plant growth is derived from Qinghai-Tibet
The brevibacterium on plateau by low altitude area and room temperature domestication and strain, then it is compound by and area's main environmental conditions phase will be used
Phosphate solubilizing bacteria and relevant auxiliary materials obtained by the taming again of respective conditions, at the same have promote low temperature, low-oxygen environment plant growth it is more
The lasting composite efficacy of weight.
Specific embodiment
The following is a preferred embodiment of the present invention, it is noted that for those skilled in the art
For, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also considered as
Protection scope of the present invention.
Embodiment 1
A kind of promotion low temperature, microorganism formulation of low-oxygen environment plant growth and preparation method thereof
Prepare raw material: 70 parts of starch, 10 parts of cold-resistant lower oxygen concentration resistance microbial bacterial agent, 20 parts of algal polysaccharides.Wherein cold-resistant is resistance to low
Oxygen animalcule microbial inoculum is the huge gemma bar that the brevibacterium derived from Qinghai-Tibet Platean obtains by taming again, phosphate solubilizing microorganism is domestication
Bacterium (the two 9:1).
Material is put into blender to mix, the revolving speed of blender is set as 180r/min, and temperature is room temperature, reaction
Time is 22min, and the microorganism formulation for promoting low temperature, low-oxygen environment plant growth is made.
Agricultural organic fertilizer is uniformly mixed when application by 1:99.
Embodiment 2
A kind of promotion low temperature, microorganism formulation of low-oxygen environment plant growth and preparation method thereof
Prepare raw material: 60 parts of starch, 8 parts of cold-resistant lower oxygen concentration resistance microbial bacterial agent, 32 parts of algal polysaccharides.Wherein cold-resistant lower oxygen concentration resistance
Microbial bacterial agent is the agrobacterium (two that the brevibacterium derived from Qinghai-Tibet Platean obtains by taming again, phosphate solubilizing microorganism is domestication
Person 8:2).
Material is put into blender to mix, the revolving speed of blender is set as 200r/min, and temperature is room temperature, reaction
Time is 20min, and the microorganism formulation for promoting low temperature, low-oxygen environment plant growth is made.
Agricultural organic fertilizer is uniformly mixed when application by 2:98.
Embodiment 3
A kind of promotion low temperature, microorganism formulation of low-oxygen environment plant growth and preparation method thereof
Prepare raw material: 68 parts of starch, 8 parts of cold-resistant lower oxygen concentration resistance microbial bacterial agent, 24 parts of algal polysaccharides.Wherein cold-resistant lower oxygen concentration resistance
Microbial bacterial agent is that the unicellular bacterium of vacation derived from Qinghai-Tibet Platean obtains by taming again, phosphate solubilizing microorganism is the huge gemma tamed
Bacillus (the two 8:2).
Material is put into blender to mix, the revolving speed of blender is set as 100r/min, and temperature is room temperature, reaction
Time is 30min, and the microorganism formulation for promoting low temperature, low-oxygen environment plant growth is made.
Embodiment 4
A kind of promotion low temperature, microorganism formulation of low-oxygen environment plant growth and preparation method thereof
Prepare raw material: 65 parts of starch, 5 parts of cold-resistant lower oxygen concentration resistance microbial bacterial agent, 30 parts of algal polysaccharides.Wherein cold-resistant lower oxygen concentration resistance
Microbial bacterial agent is the pseudomonad (two that the brevibacterium derived from Qinghai-Tibet Platean obtains by taming again, phosphate solubilizing microorganism is domestication
Person 9:1).
Material is put into blender to mix, the revolving speed of blender is set as 250r/min, and temperature is room temperature, reaction
Time is 15min, and the microorganism formulation for promoting low temperature, low-oxygen environment plant growth is made.
Embodiment 5
A kind of promotion low temperature, microorganism formulation of low-oxygen environment plant growth and preparation method thereof
Prepare raw material: 63 parts of starch, 9 parts of cold-resistant lower oxygen concentration resistance microbial bacterial agent, 28 parts of algal polysaccharides.Wherein cold-resistant lower oxygen concentration resistance
Microbial bacterial agent is derived from Qinghai-Tibet Platean by brevibacterium obtained by taming again.
Material is put into blender to mix, the revolving speed of blender is set as 280r/min, and temperature is room temperature, reaction
Time is 12min, and the microorganism formulation for promoting low temperature, low-oxygen environment plant growth is made.
Embodiment effect
Field trial is to fertilizer functional verification:
Summer in choosing the more consistent experimental plot of condition (relative low temperature low-oxygen environment) in Lhasa, is divided into 18
1 meter × 1 meter block tests each embodiment difference in plot by above-mentioned 5 embodiments and each three design iterations of blank control
It is uniformly blended into soil, normal watering Irrigation using identical deal (i.e. 100 grams), is counted after sowing each 100,10 days, green vegetables seed
Thinning after emergence rate, then 10 days respectively stays 50 plants；Harvest randomly chooses each 20 plants of titles gross weight after 30 days again.Statistical result such as the following table 1
It is described.
1 embodiment effect statistical form of table
From above-mentioned table 1 as can be seen that using embodiment 1-5 effect relative to blank control group want it is excellent very much.
The emergence rate of plant improves 30% or more, and the biomass of plant improves 30% or more, this explanation can under formula of the invention
Reach and promotes low temperature, low-oxygen environment plant growth.In addition, from 1 result of table it has also been discovered that be properly added agricultural organic fertilizer can be with
Significantly improve plant growth-promoting effect.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Sequence table
<110>Jiangsu University
<120>a kind of promotion low temperature, microorganism formulation of low-oxygen environment plant growth and preparation method thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1384
<212> DNA
<213>brevibacterium (Brevibacterium sp.)
<400> 1
ccccgccatc ggcttcgttc gaatgccatg catagcgact cgcacgccaa gcgtgcgtcc 60
ctgagccatg atcaaactct atgagtaaca cgtgggcaac ctgcctataa gactgggata 120
acttcgggaa accggagcta ataccggata cgttcttttc tcgcatgaga gaagatggaa 180
agacggttta cgctgtcact tatagatggg cccgcggcgc attagctagt tggtgaggta 240
atggctcacc aaggcgacga tgcgtagccg acctgagagg gtgatcggcc acactgggac 300
tgagacacgg cccagactcc tacgggaggc agcagtaggg aatcttccgc aatggacgaa 360
agtctgacgg agcaacgccg cgtgaacgaa gaaggccttc gggtcgtaaa gttctgttgt 420
tagggaagaa caagtaccag agtaactgct ggtaccttga cggtacctaa ccagaaagcc 480
acggctaact acgtgccagc agccgcggta atacgtaggt ggcaagcgtt gtccggaatt 540
attgggcgta aagcgcgcgc aggtggttcc ttaagtctga tgtgaaagcc cacggctcaa 600
ccgtggaggg tcattggaaa ctggggaact tgagtgcaga agaggaaagt ggaattccaa 660
agtgtagcgg tgaaatgcgt agagatttgg aggaacacca gtggcgaagg cgactttctg 720
gtctgtaact gacactgagg cgcgaaagcg tggggagcaa acaggattag ataccctggt 780
agtccacgcc gtaaacgatg agtgctaagt gttagagggt ttccgccctt tagtgctgca 840
gctaacgcat taagcactcc gcctggggag tacggccgca aggctgaaac tcaaaggaat 900
tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc 960
ttaccaggtc ttgacatcct ctgacaaccc tagagatagg gctttcccct tcgggggaca 1020
gagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc 1080
gcaacgagcg caacccttga tcttagttgc cagcattcag ttgggcactc taaggtgact 1140
gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc cttatgacct 1200
gggctacaca cgtgctacaa tggatggtac aaagggctgc aaacctgcga aggtaagcga 1260
atcccataaa gccattctca gttcggattg caggctgcaa ctcgcctgca tgaagccgga 1320
atcgctagta atcgcggatc agcatgccgc ggtgaatacg ttcccgggcc ttgtacacac 1380
cgcc 1384
Claims (7)
1. a kind of microorganism formulation for promoting low temperature, low-oxygen environment plant growth, which is characterized in that including starch, cold-resistant is resistance to low
Oxygen animalcule microbial inoculum and algal polysaccharides, according to parts by weight, 60-70 parts of starch, 8-10 parts of cold-resistant lower oxygen concentration resistance microbial bacterial agent, sea
20-32 parts of polysaccharides；
The cold-resistant lower oxygen concentration resistance microorganism fungus kind is the brevibacterium Brevibacterium sp.TS22 derived from Qinghai-Tibet Platean, is passed through
Low altitude area and room temperature are tamed and are obtained, and are preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number
Are as follows: CGMCC 16576.
2. a kind of microorganism formulation for promoting low temperature, low-oxygen environment plant growth as described in claim 1, which is characterized in that
The acclimation conditions of the low altitude area and room temperature are as follows: the TS22 strain inoculated of Qinghai-Tibetan plateau soil will be isolated from into LB culture medium,
In secondary culture, LB culture medium prescription are as follows repeatedly under room temperature: tryptone 10g/L/ yeast extract 5g/L, sodium chloride
10g/L adjusts pH to 7.4,121 DEG C of high pressure steam sterilization 20min with NaOH；
In use, must be tamed again by corresponding with area's main environmental conditions will be used condition, then acclimation conditions are as follows: will be through
The TS22 bacterial strain for crossing low altitude area and room temperature domestication, is inoculated into LB culture medium, the secondary culture repeatedly under the conditions of 10-20 DEG C, LB
Culture medium prescription is as follows: tryptone 10g/L, yeast extract 5g/L, sodium chloride 10g/L, adjusts pH to 7.4,121 with NaOH
DEG C high pressure steam sterilization 20min.
3. a kind of microorganism formulation for promoting low temperature, low-oxygen environment plant growth as described in claim 1, which is characterized in that
The cold-resistant lower oxygen concentration resistance microorganism fungus kind further includes phosphate solubilizing microorganism, and the phosphate solubilizing microorganism is bacillus megaterium, false single
One of born of the same parents bacterium or agrobacterium are a variety of；
In use, need to domestication Jing Guo condition corresponding with area's main environmental conditions will be used, acclimation conditions are as follows: by phosphate solubilizing bacteria
Strain is inoculated into Phos culture respectively and is based on carrying out strain passage culture, Phos culture medium prescription used are as follows: Portugal at 10-20 DEG C
Grape sugar 10g/L, (NH4)2SO40.5g/L, NaCl 3g/L, MgSO4·7H2O 0.5g/L, KCl 0.5g/L, FeSO4·7H2O
0.03g/L, MnSO40.03g/L, Ca3(PO4)27.2,115 DEG C of sterilizing 20min of 5g/L, pH.
4. a kind of microorganism formulation for promoting low temperature, low-oxygen environment plant growth as claimed in claim 3, which is characterized in that
The weight of the phosphate solubilizing microorganism accounts for the 0-20% of cold-resistant lower oxygen concentration resistance microbial bacterial agent.
5. the preparation method of the microorganism formulation of a kind of promotion low temperature as described in claim 1, low-oxygen environment plant growth,
It is characterized in that, specific steps include: by starch, and cold-resistant lower oxygen concentration resistance microbial bacterial agent and algal polysaccharides are put into blender and mixed
Even, the revolving speed of blender is set as 100-300r/min, and temperature is room temperature, reaction time 10-30min, be made promote low temperature,
The microorganism formulation of low-oxygen environment plant growth.
6. it is as described in claim 1 it is a kind of promote low temperature, low-oxygen environment plant growth microorganism formulation for promote low temperature,
The purposes of low-oxygen environment plant growth, which is characterized in that the microorganism formulation list for promoting low temperature, low-oxygen environment plant growth
It solely uses or agroforestry organic fertilizer uses.
7. purposes as claimed in claim 6, which is characterized in that promote the microorganism formulation of low temperature, low-oxygen environment plant growth
Agroforestry organic fertilizer is in use, the microorganism formulation and agricultural organic fertilizers for promoting low temperature, low-oxygen environment plant growth
Mass ratio is 2-4:98-96.
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