CN105950712A - System for identification or assisted identification of microorganisms in beer production process and application thereof - Google Patents

System for identification or assisted identification of microorganisms in beer production process and application thereof Download PDF

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CN105950712A
CN105950712A CN201610265317.7A CN201610265317A CN105950712A CN 105950712 A CN105950712 A CN 105950712A CN 201610265317 A CN201610265317 A CN 201610265317A CN 105950712 A CN105950712 A CN 105950712A
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water
sequence
cultivates
bacillus
characteristic
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郭立芸
谢鑫
梁云
王梅
林智平
贾凤超
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Yanjing Beer Co Ltd Beijing
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Yanjing Beer Co Ltd Beijing
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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Abstract

The invention discloses a system for identification or assisted identification of microorganisms in a beer production process and an application thereof. The invention provides the method for identification or assisted identification of beer contaminant bacteria, wherein the method comprises the following steps: (1) separating and purifying the microorganisms in a sample, to obtain a strain; (2) detecting morphological characteristics and physiological and biochemical characteristics of the strain; (3) extracting genomic DNA of the strain, to obtain a conserved DNA sequence; and (4) comparing the morphological characteristics and the physiological and biochemical characteristics of the strain and the conserved DNA sequence with microbial information recorded in a database, determining whether the strain is beer contaminant bacteria, and if the strain is the beer contaminant bacteria, determining a specific name of the strain, wherein the sample is taken from any link of the beer production process. By comparison with the characteristics of the contaminant bacteria existing in the database and combination of the distribution characteristics of population in areas, seasons and the production process, the identification result of the contaminant bacteria is quickly obtained.

Description

Identify or assist system and the application thereof identifying beer production microorganism
Technical field
The present invention relates to microorganism field is identified or assisted system and the application thereof identifying beer production contaminating microorganisms.
Background technology
Beer Production is the process that a pure species yeast is brewageed, and the pollution of any antibacterial and external wild yeasts the most likely brings Deterioration on beer quality, each brewery in long-term production process, due to residing geographical position, climatic environment and The difference of microorganism management, has defined oneself " small-sized ecosystem ", and has defined " ecological balance " to a certain extent.
The medicated beer major part polluted by harmful bacteria can be putrid and deteriorated, its main contaminated bacteria have lactic acid bacteria, acetic acid bacteria, intestinal bacteria, Anaerobic fermentation Zymomonas mobilis and some wild yeasts etc., medicated beer can produce multiple metabolite after being polluted by microorganism, its metabolite Stay in medicated beer with cellular content so that different miscellaneous taste occurs in medicated beer.
Brewery's control of microorganisms is great to the relationship between quality of medicated beer.Control of microorganisms is bad, not only affects yeast activity, blood sugar lowering Ability declines, and yeast coherency is deteriorated, and pH value changes, and makes biacetyl reduction rate slow down, and can produce many by-products, Causing beer flavor to change, even if some contaminated bacterias do not enter in finished wine, but its metabolite still can be stayed in medicated beer and produce Raw adverse effect, therefore control of microorganisms should run through production overall process.Control for brewery's microorganism should be set up to micro-life On the basis of thing contamination class and distribution situation are fully held, on the premise of determining the kind of contaminated bacteria, understand its life spy Levy, analyze the harm that may bring, be conducive to that source is polluted in medicated beer microorganism and review, and take effective prophylactico-therapeutic measures.
Moreover, owing to climatic environment and the other countries area of China zones of different have difference, Chinese beer produces Enterprise's Species structure of contaminating microorganisms in beer production is also discrepant with other countries areas, therefore, sets up symbol The contaminating microorganisms data base closing China beer production contamination characteristics has directive significance, on the one hand, can make The technique of Domestic Beer manufacturing enterprise and quality control officer fully understand the kind of this enterprise major microorganisms and in each operation Distribution situation, improves specific aim and the self-confidence of microorganism management；On the other hand, by utilizing and in contaminated bacteria data bacterium storehouse Identify collection of illustrative plates comparison, be conducive to beer spoilage bacteria is carried out rapid preliminary judgement, understand the microbiology turbidity occurred in operation, and Time take measures.Further, along with the foundation of contaminated bacteria data base, the qualification of contaminated bacteria and the standardization of store method, can be progressively Improve the microorganism resource of Chinese beer contaminating microorganisms, improve the overall understanding of microorganism and control level, for improving product quality Lay a solid foundation.
Summary of the invention
Solve the technical problem that it is how to simplify the identity process of microorganism in beer production, shortening qualification required by the present invention Cycle, effectively control pollute.
In order to solve above-mentioned technical problem, the invention provides a kind of qualification or the system of auxiliary qualification medicated beer microorganism, including beer The DNA sample of contaminating microorganisms and the data base of the described contaminating microorganisms information of record in wine production process；Described contaminating microorganisms Information includes 1)-4) at least one:
1) conserved DNA sequences of described contaminating microorganisms；
2) morphological feature of described contaminating microorganisms；
3) physiological and biochemical property of described contaminating microorganisms；
4) characteristic distributions of described microorganism.
Described characteristic distributions includes season, region and the production process that contaminating microorganisms occurs.
In said system, described season be throughout the year at least one season, such as summer or winter；Described region is at least one district Territory, such as area, North China, East China, South China or the Northwest；Described production process is at least one operation, as CIP disinfection system, saccharifying, ferment, filter or pack.
In said system, contaminating microorganisms described in described data base includes 71 strain aerobic bacteria, 15 strain anaerobic bacterias and 3 strains Fungus.
In said system, described 71 strain aerobic bacteria include: Klebsiella pneumonia ozena subspecies (Klebsiella pneumoniae subsp.ozaenae)、Serratia marcescens subsp.Sakuensis、Aquitalea magnusonii、Chryseobacterium arthrosphaerae、Enterobacter xiangfangensis、 Macrococcus equipercicus, Ralstonia insidiosa, enterobacter asburiae (Enterobacter asburiae), Acinetobacter baumannii (Acinetobacter baumannii), Acinetobacter nosocomialis, the product golden yellow bar of indole Bacterium (Chryseobacterium indologenes), Xanthomonas sacchari, He Shi Escherichia (Escherichia Hermannii), bacillus amyloliquefaciens solution starch subspecies (Bacillus amyloliquefaciens subsp. Amyloliquefaciens), bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens Subsp.plantarum), giant cell nitrogen Zymomonas mobilis (Azomonas macrocytogenes), radioprotective acinetobacter calcoaceticus (Acinetobacter radioresistens), Bacillus subtilis subsp.inaquosorum, waxy spore bar Bacterium (Bacillus cereus), Klebsiella quasipneumoniae subsp.quasipneumoniae, Ludwig Enterobacteria (Enterobacter ludwigii), staphylococcus xylosus (Staphylococcus xylosus), Pi Te not lever Bacterium (Acinetobacter pittii), roost rose of Sharon pseudomonas (Pseudomonas hibiscicola), micrococcus lacticus (Micrococcus lactis), husky good fortune bacillus cereus (Bacillus safensis), basophilic Exiguobacterium sp (Exiguobacterium alkaliphilum), aerosporus (Bacillus aerophilus), Burkholderia Arboris, anthrax bacillus (Bacillus anthracis), Bacillus tequilensis, soil bacillus brevis (Brevibacillus agri)、Exiguobacterium indicum、Deinococcus ficus、Bacillus Siamensis, Xin Shi acinetobacter calcoaceticus (Acinetobacter schindleri), Young citric acid bacillus (Citrobacter Youngae), Deinococcus radioduran, Bacillus infantis, Vietnam bulkholderia cepasea (Burkholderia vietnamiensis)、Bacillus aryabhattai、Enterobacter mori、Cronobacter dublinensis Subsp.Lausannensis, Pantoea calida, excrement shortwave Zymomonas mobilis (Brevundimonas faecalis), saprophytic Staphylococcus (Staphylococcus saprophyticus subsp.Saprophyticus), China Al Kut Salmonella (Kurthia in the last of the ten Heavenly stems Huakuii), Kurthia gibsonii (Kurthia gibsonii), food methanol bacillus cereus (Bacillus Methylotrophicus), Pseudomonas graminis, simple bacillus cereus (Bacillus simplex), Lysinibacillus xylanilyticus、Chryseobacterium hispalense、Kocuria marina、 Pseudomonas kunmingensis, Paenibacillus humicus, U.S. bar bacterium (Corynebacterium Callunae), Cedecea neteri (Cedecea neteri), brevibacterium frigoritolerans (Brevibacterium Frigoritolerans), lemon yellow bacillus pumilis (Curtobacterium citreum), Bacillus panaciterrae, Chryseobacterium hominis, sphingomonas paucimobilis (Sphingomonas paucimobilis), Erwinia Tasmaniensis, Pseudomonas aeruginosa (Pseudomonas aeruginosa), pseudo-blood Sphingol single-cell (Sphingomonas Pseudosanguinis), nicotinic acid bacillus cereus (Bacillus niacini), acetyl Exiguobacterium sp (Exiguobacterium Acetylicum), Deinococcus caeni, Deinococcus grandis, plant bacillus pumilis (Curtobacterium plantarum)；
Described 15 strain anaerobic bacterias include: Leuconostoc mesenteroides dextran subspecies (Leuconostoc mesenteroides Subsp.dextranicun), Lactobacillus brevis (Lactobacillus brevis), Lactococcus garvieae (Lactococcus Garvieae), Lactobacillus paracasei subsp.tolerans, cibarium Wei Si Salmonella (Weissella cibaria), Pediococcus claussenii, Pediococcus lolii, leuconostoc lactis (Leuconostoc holzapfelii), Lactococcus lactis subsp.tructae, lactococcus lactis subsp.cremoris (Lactococcus lactis Subsp.cremoris), Pediococcus pentosaceus (Pediococcus pentosaceus), Lactobacillus plantarum (Lactobacillus Plantarum subsp.plantarum), leuconostoc pseudomesenteroides (Leuconostoc pseudomesenteroides), visit Family name clostridium (Clostridium beijerinckii), solution ornithine Raoul bacterium (Raoultella ornithinolytica)；
Described 3 fungal strains include: Aureobasidium, rhodotorula mucilaginosa (Rhodotorula mucilaginosa), Cryptococcus rajasthanensis。
In said system, the conserved DNA sequences of described aerobic bacteria and described anaerobic bacteria is 16s rDNA sequence；Described fungus Conserved DNA sequences be ITS sequence.
In said system, described in described data base described contaminating microorganisms information such as A1-A71, B1-B15 and C1-C3 institute Show；A1-A71 is the information of described 71 kinds of aerobic bacteria；B1-B15 is the information of described 15 kinds of anaerobic bacterias；C1-C3 is institute State the information of 3 kinds of funguses；Specifying information refers to embodiment 1.
In said system, described data base has also stated that the probability of happening of described contaminating microorganisms.
The probability of happening of described contaminating microorganisms is for when production process occurs that microorganism is polluted, and contaminating microorganisms is certain microorganism Probability.
Described production process is the production process having occurred to pollute.
In said system, the probability of happening of described contaminating microorganisms refers to embodiment 1.
North China involved in the present invention includes Beijing, Inner Mongolia Autonomous Region, Hebei province, Shanxi Province, Shandong Province, the Liao Dynasty Ning Sheng, Heilongjiang Province and Jilin Province.
East China involved in the present invention include Fujian Province, Zhejiang Province, Hunan Province, Hubei Province, Jiangxi Province, Henan Province, Anhui Province, Jiangsu Province and Shanghai City.
Southeast involved in the present invention include Guangxi Zhuang Autonomous Region, Guangdong Province, Yunnan Province, Guizhou Province, Sichuan Province and Chongqing City.
The Northwest involved in the present invention includes Xinjiang Uygur Autonomous Regions, Tibet Autonomous Region, Ningxia Hui Autonomous Region, green grass or young crops Hai Sheng, Gansu Province and Shaanxi Province.
In order to solve above-mentioned technical problem, the present invention also provides for said system in the application identified or in auxiliary qualification brewery sludge.
In order to solve above-mentioned technical problem, the present invention provides again a kind of and identifies or the method for auxiliary qualification brewery sludge.
Said method comprises the steps:
(1) microorganism in isolated and purified sample, obtains bacterial strain；
(2) morphological characteristic and the physiological and biochemical property of described bacterial strain are detected；
(3) extract the genomic DNA of described bacterial strain, obtain conserved DNA sequences；
(4) by the morphological characteristic of described bacterial strain, physiological and biochemical property and described conserved DNA sequences and number described in said system The microbial information recorded according to storehouse is compared, and determines whether described bacterial strain is brewery sludge, if described bacterial strain is contaminated bacteria, Determine the kind name of described bacterial strain；
Any link of beer production taken from by described sample.
In said method, described step (4) also includes the genomic DNA of described bacterial strain that obtains in step (3) with above-mentioned Described in system, in beer production, the DNA sample of contaminating microorganisms hybridizes.
In said method, described step (4) also includes the probability of happening determining described bacterial strain according to comparison result.
The invention has the beneficial effects as follows: owing to climate in china is different with American-European countries from geographical position, the Beer Production of China The Species structure of process contamination microorganism is also different from other countries, and the present invention is first at China's main medicated beer in four, nationwide Produce close quarters (North China, East China, the southeast, northwest) point to have carried out the brewery of zones of different producing overall process season Microbiological data storehouse build, can be compared by contaminated bacteria feature existing with data base, in conjunction with each region, each season and The pollution Species structure feature of production process, quickly draws the qualification result of contaminated bacteria.For Chinese beer manufacturing enterprise Beer Production The foundation of the process contamination dientification of bacteria, hazard analysis, technology controlling and process, contaminating microorganisms prevention and control and monitoring system provides number According to supporting and theoretical direction.
Accompanying drawing explanation
Fig. 1 is the colony characteristics of Klebsiella pneumonia ozena subspecies (Klebsiella pneumoniae subsp.ozaenae) And cell characteristic.Wherein, A is colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Fig. 2 is colony characteristics and the cell characteristic of Serratia marcescens subsp.Sakuensis.Wherein, A is bacterium Fall feature；B is the cell characteristic under × 1000 power microscopes.
Fig. 3 is colony characteristics and the cell characteristic of Aquitalea magnusonii.Wherein, A is colony characteristics；B be × Cell characteristic under 1000 power microscopes.
Fig. 4 is colony characteristics and the cell characteristic of Chryseobacterium arthrosphaerae.Wherein, A is that bacterium colony is special Levy；B is the cell characteristic under × 1000 power microscopes.
Fig. 5 is colony characteristics and the cell characteristic of Enterobacter xiangfangensis.Wherein, A is colony characteristics；B For the cell characteristic under × 1000 power microscopes.
Fig. 6 is colony characteristics and the cell characteristic of Macrococcus equipercicus.Wherein, A is colony characteristics；B is Cell characteristic under × 1000 power microscopes.
Fig. 7 is colony characteristics and the cell characteristic of Ralstonia insidiosa.Wherein, A is colony characteristics；B is × 1000 Cell characteristic under power microscope.
Fig. 8 is colony characteristics and the cell characteristic of enterobacter asburiae (Enterobacter asburiae).Wherein, A is bacterium colony Feature；B is the cell characteristic under × 1000 power microscopes.
Fig. 9 is colony characteristics and the cell characteristic of Acinetobacter baumannii (Acinetobacter baumannii).Wherein, A is Colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 10 is colony characteristics and the cell characteristic of Acinetobacter nosocomialis.Wherein, A is colony characteristics；B For the cell characteristic under × 1000 power microscopes.
Figure 11 is colony characteristics and the cell characteristic of chryseobacterium indologenes (Chryseobacterium indologenes).Its In, A is colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 12 is colony characteristics and the cell characteristic of Xanthomonas sacchari.Wherein, A is colony characteristics；B be × Cell characteristic under 1000 power microscopes.
Figure 13 is colony characteristics and the cell characteristic of He Shi Escherichia (Escherichia hermannii).Wherein, A is Colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 14 is bacillus amyloliquefaciens solution starch subspecies (Bacillus amyloliquefaciens subsp. Amyloliquefaciens) colony characteristics and cell characteristic.Wherein, A is colony characteristics；B is at × 1000 power microscopes Under cell characteristic.
Figure 15 is bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) Colony characteristics and cell characteristic.Wherein, A is colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 16 is colony characteristics and the cell characteristic of giant cell nitrogen Zymomonas mobilis (Azomonas macrocytogenes).Wherein, A For colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 17 is colony characteristics and the cell characteristic of radioprotective acinetobacter calcoaceticus (Acinetobacter radioresistens).Its In, A is colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 18 is colony characteristics and the cell characteristic of Bacillus subtilis subsp.inaquosorum.Wherein, A is bacterium Fall feature；B is the cell characteristic under × 1000 power microscopes.
Figure 19 is colony characteristics and the cell characteristic of bacillus cereus (Bacillus cereus).Wherein, A is that bacterium colony is special Levy；B is the cell characteristic under × 1000 power microscopes.
Figure 20 is colony characteristics and the cell characteristic of Klebsiella quasipneumoniae subsp.quasipneumoniae. Wherein, A is colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 21 is colony characteristics and the cell characteristic of Ludwig enterobacteria (Enterobacter ludwigii).Wherein, A For colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 22 is colony characteristics and the cell characteristic of staphylococcus xylosus (Staphylococcus xylosus).Wherein, A is Colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 23 is colony characteristics and the cell characteristic of skin spy's acinetobacter calcoaceticus (Acinetobacter pittii).Wherein, A is bacterium Fall feature；B is the cell characteristic under × 1000 power microscopes.
Figure 24 is colony characteristics and the cell characteristic of roost rose of Sharon pseudomonas (Pseudomonas hibiscicola).Wherein, A For colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 25 is colony characteristics and the cell characteristic of micrococcus lacticus (Micrococcus lactis).Wherein, A is colony characteristics； B is the cell characteristic under × 1000 power microscopes.
Figure 26 is colony characteristics and the cell characteristic of husky good fortune bacillus cereus (Bacillus safensis).Wherein, A is bacterium colony Feature；B is the cell characteristic under × 1000 power microscopes.
Figure 27 is colony characteristics and the cell characteristic of basophilic Exiguobacterium sp (Exiguobacterium alkaliphilum).Wherein, A is colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 28 is colony characteristics and the cell characteristic of aerosporus (Bacillus aerophilus).Wherein, A is bacterium Fall feature；B is the cell characteristic under × 1000 power microscopes.
Figure 29 is colony characteristics and the cell characteristic of Burkholderia arboris.Wherein, A is colony characteristics；B be × Cell characteristic under 1000 power microscopes.
Figure 30 is colony characteristics and the cell characteristic of anthrax bacillus (Bacillus anthracis).Wherein, A is colony characteristics； B is the cell characteristic under × 1000 power microscopes.
Figure 31 is colony characteristics and the cell characteristic of Bacillus tequilensis.Wherein, A is colony characteristics；B be × Cell characteristic under 1000 power microscopes.
Figure 32 is colony characteristics and the cell characteristic of soil bacillus brevis (Brevibacillus agri).Wherein, A is bacterium Fall feature；B is the cell characteristic under × 1000 power microscopes.
Figure 33 is colony characteristics and the cell characteristic of Exiguobacterium indicum.Wherein, A is colony characteristics；B is Cell characteristic under × 1000 power microscopes.
Figure 34 is colony characteristics and the cell characteristic of Deinococcus ficus.Wherein, A is colony characteristics；B is × 1000 Cell characteristic under power microscope.
Figure 35 is colony characteristics and the cell characteristic of Bacillus siamensis.Wherein, A is colony characteristics；B is × 1000 Cell characteristic under power microscope.
Figure 36 is colony characteristics and the cell characteristic of Xin Shi acinetobacter calcoaceticus (Acinetobacter schindleri).Wherein, A For colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 37 is colony characteristics and the cell characteristic of Young citric acid bacillus (Citrobacter youngae).Wherein, A is bacterium Fall feature；B is the cell characteristic under × 1000 power microscopes.
Figure 38 is colony characteristics and the cell characteristic of Deinococcus radioduran.Wherein, A is colony characteristics；B be Cell characteristic under × 1000 power microscopes.
Figure 39 is colony characteristics and the cell characteristic of Bacillus infantis.Wherein, A is colony characteristics；B is × 1000 Cell characteristic under power microscope.
Figure 40 is colony characteristics and the cell characteristic of Vietnam's bulkholderia cepasea (Burkholderia vietnamiensis). Wherein, A is colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 41 is colony characteristics and the cell characteristic of Bacillus aryabhattai.Wherein, A is colony characteristics；B be × Cell characteristic under 1000 power microscopes.
Figure 42 is colony characteristics and the cell characteristic of Enterobacter mori.Wherein, A is colony characteristics；B is × 1000 Cell characteristic under power microscope.
Figure 43 is colony characteristics and the cell characteristic of Cronobacter dublinensis subsp.Lausannensis.Wherein, A is colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 44 is colony characteristics and the cell characteristic of Pantoea calida.Wherein, A is colony characteristics；B is × 1000 Cell characteristic under power microscope.
Figure 45 is colony characteristics and the cell characteristic of excrement shortwave Zymomonas mobilis (Brevundimonas faecalis).Wherein, A is Colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 46 is the bacterium of staphylococcus saprophyticus (Staphylococcus saprophyticus subsp.Saprophyticus) The feature that falls and cell characteristic.Wherein, A is colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 47 is colony characteristics and the cell characteristic of China Al Kut Salmonella in the last of the ten Heavenly stems (Kurthia huakuii).Wherein, A is that bacterium colony is special Levy；B is the cell characteristic under × 1000 power microscopes.
Figure 48 is colony characteristics and the cell characteristic of Kurthia gibsonii (Kurthia gibsonii).Wherein, A is that bacterium colony is special Levy；B is the cell characteristic under × 1000 power microscopes.
Figure 49 is for eating colony characteristics and the cell characteristic of methanol bacillus cereus (Bacillus methylotrophicus).Wherein, A is colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 50 is colony characteristics and the cell characteristic of Pseudomonas graminis.Wherein, A is colony characteristics；B be × Cell characteristic under 1000 power microscopes.
Figure 51 is colony characteristics and the cell characteristic of simple bacillus cereus (Bacillus simplex).Wherein, A is that bacterium colony is special Levy；B is the cell characteristic under × 1000 power microscopes.
Figure 52 is colony characteristics and the cell characteristic of Lysinibacillus xylanilyticus.Wherein, A is colony characteristics； B is the cell characteristic under × 1000 power microscopes.
Figure 53 is colony characteristics and the cell characteristic of Chryseobacterium hispalense.Wherein, A is colony characteristics； B is the cell characteristic under × 1000 power microscopes.
Figure 54 is colony characteristics and the cell characteristic of Kocuria marina.Wherein, A is colony characteristics；B is × 1000 Cell characteristic under power microscope.
Figure 55 is colony characteristics and the cell characteristic of Pseudomonas kunmingensis.Wherein, A is colony characteristics；B is Cell characteristic under × 1000 power microscopes.
Figure 56 is colony characteristics and the cell characteristic of Paenibacillus humicus.Wherein, A is colony characteristics；B be Cell characteristic under × 1000 power microscopes.
Figure 57 is colony characteristics and the cell characteristic of U.S. bar bacterium (Corynebacterium callunae).Wherein, A is bacterium Fall feature；B is the cell characteristic under × 1000 power microscopes.
Figure 58 is colony characteristics and the cell characteristic of Cedecea neteri (Cedecea neteri).Wherein, A is colony characteristics； B is the cell characteristic under × 1000 power microscopes.
Figure 59 is colony characteristics and the cell characteristic of brevibacterium frigoritolerans (Brevibacterium frigoritolerans).Wherein, A is colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 60 is colony characteristics and the cell characteristic of lemon yellow bacillus pumilis (Curtobacterium citreum).Wherein, A For colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 61 is colony characteristics and the cell characteristic of Bacillus panaciterrae.Wherein, A is colony characteristics；B be Cell characteristic under × 1000 power microscopes.
Figure 62 is colony characteristics and the cell characteristic of Chryseobacterium hominis.Wherein, A is colony characteristics；B is Cell characteristic under × 1000 power microscopes.
Figure 63 is colony characteristics and the cell characteristic of sphingomonas paucimobilis (Sphingomonas paucimobilis).Its In, A is colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 64 is colony characteristics and the cell characteristic of Erwinia tasmaniensis.Wherein, A is colony characteristics；B be × Cell characteristic under 1000 power microscopes.
Figure 65 is colony characteristics and the cell characteristic of Pseudomonas aeruginosa (Pseudomonas aeruginosa).Wherein, A is Colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 66 is colony characteristics and the cell characteristic of pseudo-blood Sphingol single-cell (Sphingomonas pseudosanguinis). Wherein, A is colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 67 is colony characteristics and the cell characteristic of nicotinic acid bacillus cereus (Bacillus niacini).Wherein, A is that bacterium colony is special Levy；B is the cell characteristic under × 1000 power microscopes.
Figure 68 is colony characteristics and the cell characteristic of acetyl Exiguobacterium sp (Exiguobacterium acetylicum).Wherein, A is colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 69 is colony characteristics and the cell characteristic of Deinococcus caeni.Wherein, A is colony characteristics；B is × 1000 Cell characteristic under power microscope.
Figure 70 is colony characteristics and the cell characteristic of Deinococcus grandis.Wherein, A is colony characteristics；B be × Cell characteristic under 1000 power microscopes.
Figure 71 is colony characteristics and the cell characteristic of plant bacillus pumilis (Curtobacterium plantarum).Wherein, A For colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 72 is Leuconostoc mesenteroides dextran subspecies (Leuconostoc mesenteroides Subsp.dextranicun) colony characteristics and cell characteristic.Wherein, A is colony characteristics；B is at × 1000 power microscopes Under cell characteristic.
Figure 73 is colony characteristics and the cell characteristic of Lactobacillus brevis (lactobacillus brevis).Wherein, A is that bacterium colony is special Levy；B is the cell characteristic under × 1000 power microscopes.
Figure 74 is colony characteristics and the cell characteristic of Lactococcus garvieae (Lactococcus garvieae).Wherein, A is bacterium colony Feature；B is the cell characteristic under × 1000 power microscopes.
Figure 75 is colony characteristics and the cell characteristic of Lactobacillus paracasei subsp.tolerans.Wherein, A For colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 76 is colony characteristics and the cell characteristic of cibarium Wei Si Salmonella (Weissella cibaria).Wherein, A is bacterium colony Feature；B is the cell characteristic under × 1000 power microscopes.
Figure 77 is colony characteristics and the cell characteristic of Pediococcus claussenii.Wherein, A is colony characteristics；B be Cell characteristic under × 1000 power microscopes.
Figure 78 is colony characteristics and the cell characteristic of Pediococcus lolii.Wherein, A is colony characteristics；B is × 1000 Cell characteristic under power microscope.
Figure 79 is colony characteristics and the cell characteristic of leuconostoc lactis (Leuconostoc holzapfelii).Wherein, A is Colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 80 is colony characteristics and the cell characteristic of Lactococcus lactis subsp.tructae.Wherein, A is bacterium colony Feature；B is the cell characteristic under × 1000 power microscopes.
Figure 81 is that the colony characteristics of lactococcus lactis subsp.cremoris (Lactococcus lactis subsp.cremoris) is with thin Born of the same parents' feature.Wherein, A is colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 82 is colony characteristics and the cell characteristic of Pediococcus pentosaceus (Pediococcus pentosaceus).Wherein, A is Colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 83 is the colony characteristics of Lactobacillus plantarum plant subspecies (Lactobacillus plantarum subsp.plantarum) And cell characteristic.Wherein, A is colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 84 is colony characteristics and the cell characteristic of leuconostoc pseudomesenteroides (Leuconostoc pseudomesenteroides). Wherein, A is colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 85 is colony characteristics and the cell characteristic of Clostridium beijerinckii (Clostridium beijerinckii).Wherein, A is bacterium Fall feature；B is the cell characteristic under × 1000 power microscopes.
Figure 86 is for solving colony characteristics and the cell characteristic of ornithine Raoul bacterium (Raoultella ornithinolytica).Its In, A is colony characteristics；B is the cell characteristic under × 1000 power microscopes.
Figure 87 is colony characteristics and the cell characteristic of Aureobasidium.Wherein, A is colony characteristics；B is at × 1000 times Cell characteristic under microscope.
Figure 88 is colony characteristics and the cell characteristic of Rhodotorula mucilaginosa.Wherein, A is colony characteristics；B is Cell characteristic under × 1000 power microscopes.
Figure 89 is colony characteristics and the cell characteristic of Cryptococcus rajasthanensis.Wherein, A is colony characteristics； B is the cell characteristic under × 1000 power microscopes.
Figure 90 is the division schematic diagram of North China, East China, the southeast and the Northwest.
Detailed description of the invention
Being further described in detail the present invention below in conjunction with detailed description of the invention, the embodiment be given is only for illustrating this Invent rather than in order to limit the scope of the present invention.Experimental technique in following embodiment, if no special instructions, is often Rule method.Material used in following embodiment, reagent etc., if no special instructions, the most commercially obtain.
Embodiment 1, identify or assist the system of microorganism in beer production of identifying
A kind of qualification or auxiliary identify that in beer production, the system of microorganism includes described Klebsiella pneumonia ozena subspecies (Klebsiella pneumoniae subsp.ozaenae)、Serratia marcescens subsp.Sakuensis、 Aquitalea magnusonii、Chryseobacterium arthrosphaerae、Enterobacter xiangfangensis、 Macrococcus equipercicus, Ralstonia insidiosa, enterobacter asburiae (Enterobacter asburiae), Acinetobacter baumannii (Acinetobacter baumannii), Acinetobacter nosocomialis, the product golden yellow bar of indole Bacterium (Chryseobacterium indologenes), Xanthomonas sacchari, He Shi Escherichia (Escherichia Hermannii), bacillus amyloliquefaciens solution starch subspecies (Bacillus amyloliquefaciens subsp. Amyloliquefaciens), bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens Subsp.plantarum), giant cell nitrogen Zymomonas mobilis (Azomonas macrocytogenes), radioprotective acinetobacter calcoaceticus (Acinetobacter radioresistens), Bacillus subtilis subsp.inaquosorum, waxy spore bar Bacterium (Bacillus cereus), Klebsiella quasipneumoniae subsp.quasipneumoniae, Ludwig Enterobacteria (Enterobacter ludwigii), staphylococcus xylosus (Staphylococcus xylosus), Pi Te not lever Bacterium (Acinetobacter pittii), roost rose of Sharon pseudomonas (Pseudomonas hibiscicola), micrococcus lacticus (Micrococcus lactis), husky good fortune bacillus cereus (Bacillus safensis), basophilic Exiguobacterium sp (Exiguobacterium alkaliphilum), aerosporus (Bacillus aerophilus), Burkholderia Arboris, anthrax bacillus (Bacillus anthracis), Bacillus tequilensis, soil bacillus brevis (Brevibacillus agri)、Exiguobacterium indicum、Deinococcus ficus、Bacillus Siamensis, Xin Shi acinetobacter calcoaceticus (Acinetobacter schindleri), Young citric acid bacillus (Citrobacter Youngae), Deinococcus radioduran, Bacillus infantis, Vietnam bulkholderia cepasea (Burkholderia vietnamiensis)、Bacillus aryabhattai、Enterobacter mori、Cronobacter dublinensis Subsp.Lausannensis, Pantoea calida, excrement shortwave Zymomonas mobilis (Brevundimonas faecalis), saprophytic Staphylococcus (Staphylococcus saprophyticus subsp.Saprophyticus), China Al Kut Salmonella (Kurthia in the last of the ten Heavenly stems Huakuii), Kurthia gibsonii (Kurthia gibsonii), food methanol bacillus cereus (Bacillus Methylotrophicus), Pseudomonas graminis, simple bacillus cereus (Bacillus simplex), Lysinibacillus xylanilyticus、Chryseobacterium hispalense、Kocuria marina、 Pseudomonas kunmingensis, Paenibacillus humicus, U.S. bar bacterium (Corynebacterium Callunae), Cedecea neteri (Cedecea neteri), brevibacterium frigoritolerans (Brevibacterium Frigoritolerans), lemon yellow bacillus pumilis (Curtobacterium citreum), Bacillus panaciterrae, Chryseobacterium hominis, sphingomonas paucimobilis (Sphingomonas paucimobilis), Erwinia Tasmaniensis, Pseudomonas aeruginosa (Pseudomonas aeruginosa), pseudo-blood Sphingol single-cell (Sphingomonas Pseudosanguinis), nicotinic acid bacillus cereus (Bacillus niacini), acetyl Exiguobacterium sp (Exiguobacterium Acetylicum), Deinococcus caeni, Deinococcus grandis, plant bacillus pumilis (Curtobacterium Plantarum), Leuconostoc mesenteroides dextran subspecies (Leuconostoc mesenteroides Subsp.dextranicun), Lactobacillus brevis (Lactobacillus brevis), Lactococcus garvieae (Lactococcus Garvieae), Lactobacillus paracasei subsp.tolerans, cibarium Wei Si Salmonella (Weissella cibaria), Pediococcus claussenii, Pediococcus lolii, leuconostoc lactis (Leuconostoc holzapfelii), Lactococcus lactis subsp.tructae, lactococcus lactis subsp.cremoris (Lactococcus lactis Subsp.cremoris), Pediococcus pentosaceus (Pediococcus pentosaceus), Lactobacillus plantarum (Lactobacillus Plantarum subsp.plantarum), leuconostoc pseudomesenteroides (Leuconostoc pseudomesenteroides), visit Family name clostridium (Clostridium beijerinckii), solve ornithine Raoul bacterium (Raoultella ornithinolytica), Aureobasidium, rhodotorula mucilaginosa (Rhodotorula mucilaginosa) and Cryptococcus rajasthanensis Deng the DNA sample of 89 kinds of microorganisms and carry the data base of these microbial information and these microorganism probability of happening.Wherein Microbial information is as follows:
A1 is Klebsiella pneumonia ozena subspecies (Klebsiella pneumoniae subsp.ozaenae)；
Strain classification: aerobic bacteria；
Condition of culture: plate count culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Fig. 1): milky, circular, neat in edge, surface is glossy, moistening, easily provokes, is soluble in Water；
Cell characteristic (B in Fig. 1): quarter butt, size 0.5～0.8 μ m 1～2 μm, individually, become double or short catenation； Atrichia without brood cell, does not moves, and has thicker pod membrane, and majority has pili；
Physiological and biochemical property: Gram-negative, KOH wire drawing is positive, produces the 2,3-butanediol main end as glucose fermentation End product, VP tests the positive；Form few lactic acid, acetic acid and formic acid with mixed acid fermentation, form many ethanol；Fermentation inositol, Hydrolysis carbamide, does not produce ODC Ornithine decarboxylase or H2S；
DNA sequence is as shown in sequence 1；
Characteristic distributions: summer, East China, high bubble phase fermentation liquid.
A2 is Serratia marcescens subsp.Sakuensis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Fig. 2): milky, circular, smooth surface, glossy, neat in edge, easy picking, it is soluble in Water；
Cell characteristic (B in Fig. 2): quarter butt, individually or in pairs exists, does not moves；
DNA sequence is as shown in sequence 2 in sequence table；
Characteristic distributions: summer, North China, cross drainage.
A3 is Aquitalea magnusonii；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Fig. 3): yellow is translucent, circular, neat in edge, smooth surface, glossy, easy picking, easily It is dissolved in water；
Cell characteristic (B in Fig. 3): quarter butt, individually or in pairs exists, motion；
Physiological and biochemical property: Gram-negative, KOH string test is positive, and catalase positive, oxidase positive, to chlorine Change sodium sensitive, belong to Neisseriaceae；
DNA sequence is as shown in sequence 3；
Characteristic distributions: summer, North China, dilution outlet deoxygenated water.
A4 is Chryseobacterium arthrosphaerae；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Fig. 4): yellow, petite, circular, neat in edge, smooth surface, glossy, easy picking, Soluble in water；
Cell characteristic (B in Fig. 4): quarter butt, individualism, does not moves, without spore, and 1 μ m 2 μm；
Physiological and biochemical property: Gram-negative, the KOH wire drawing experiment positive, oxidase positive, catalase positive, it is impossible to Growing at 4 DEG C and 45 DEG C, glucose fermentation, maltose, mannose produces acid, lactose fermenters, sucrose, sorbitol etc. on a small quantity Do not produce acid；
DNA sequence is as shown in sequence 4；
Characteristic distributions: summer, southeast, do not sterilize rice wine.
A5 is Enterobacter xiangfangensis；
Strain classification: aerobic bacteria:
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C:
Colony characteristics (A in Fig. 5): yellow (the most orange red), flat sheet-like, Intermediate Gray white, edge is uneven, surface Smooth, moistening, easily provoke, soluble in water；
Cell characteristic (B in Fig. 5): quarter butt, does not moves；
Physiological and biochemical property: Enterobacter xiangfangensis is that a strain is found in Heilongjiang Province, the leather separated in acid bread Lan Shi negative bacterium；
DNA sequence is as shown in sequence 5；
Characteristic distributions: summer, North China, rice wine liquid.
A6 is Macrococcus equipercicus；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Fig. 6): crocus, circular, neat in edge, smooth surface, glossy, easy picking, it is soluble in Water；
Cell characteristic (B in Fig. 6): cell spheroid, diameter 1.0～2.0 μm, individually or in pairs exist, accidental tetrad, no Motion；
Physiological and biochemical property: Gram-positive is variable to gram；With few flagellar movement；Do not sprout spore；Aerobic；Breathe generation The chemoheterotrophic bacteria thanked；Catalase is positive；The suitableeest 30～37 DEG C；Medium addicted to salt, culture medium need to add 0.5%～20%NaCl；Send out Now in the soil of sea and saliferous；
DNA sequence is as shown in sequence 6；
Characteristic distributions: summer, North China, packing shop surrounding air.
A7 is Ralstonia insidiosa；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Fig. 7): translucent white, circular, petite, neat in edge, smooth surface, easy picking, easily It is dissolved in water；
Cell characteristic (B in Fig. 7): middle bar, individually or in pairs exists, motion；
DNA sequence is as shown in sequence 7；
Characteristic distributions: summer, North China, deoxygenated water.
A8 is enterobacter asburiae (Enterobacter asburiae)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Fig. 8): white, circular, neat in edge, smooth surface, water moistens, glossy, easy picking, easily It is dissolved in water；
Cell characteristic (B in Fig. 8): quarter butt, individualism, motion, without spore；
DNA sequence is as shown in sequence 8；
Characteristic distributions: summer, North China, wine filling machine pipeline rice wine.
A9 is Acinetobacter baumannii (Acinetobacter baumannii)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Fig. 9): circular, milky, bacterium colony is bigger, neat in edge, and smooth surface is glossy, and water moistens, Thickness, easy picking, soluble in water；
Cell characteristic (B in Fig. 9): ball-type, individualism, does not moves；
Physiological and biochemical property: Gram-negative, KOH is positive, catalase positive
DNA sequence is as shown in sequence 9；
Characteristic distributions: summer, southeast, filter air bells laid.
A10 is Acinetobacter nosocomialis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 10): milky, circular, neat in edge, smooth surface, glossy, easy picking, readily soluble Yu Shui；
Cell characteristic (B in Figure 10): coccus, individually or in pairs exists, does not moves；
Physiological and biochemical property: Gram-negative coccobacillus, oxidase negative, power is negative, nitrate negative；
DNA sequence is as shown in sequence 10；
Characteristic distributions: summer, North China, surrounding air.
A11 is chryseobacterium indologenes (Chryseobacterium indologenes)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 11): yellow, circular, neat in edge, smooth surface, glossy, easy picking, it is soluble in Water；
Cell characteristic (B in Figure 11): middle bar, does not moves, without spore；
Physiological and biochemical property: Gram-negative, direct rod shape, end circle, usually 0.5 μ m 1.0～3.0 μm, does not moves, Fricton-tight or swimming, the most aerobic, external environment separator can be 37 DEG C of growths.At cultured on solid medium, bacterium colony is circular (straight Footpath 1～2nm), protuberance, smooth and glossy, Quan Yuan, catalase, oxidase, phosphatase are the most positive, indigestion agar, Chemoorganotrophy, is produced acid not aerogenesis by carbohydrate in low concentration protein culture medium；
DNA sequence is as shown in sequence 11；
Characteristic distributions: summer, southeast, filter settles.
A12 is Xanthomonas sacchari；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 12): lemon yellow, circular, neat in edge, smooth surface, glossy, easy picking, readily soluble Yu Shui；
Cell characteristic (B in Figure 12): quarter butt, exists in pairs, size 0.4～1.0 μ m 1.2～3.0 μm, single-ended the most raw Flagellum, motion；
Physiological and biochemical property: gram negative bacteria, KOH wire drawing is positive, and oxydase reaction is weak or negative, catalase positive, specially Property aerobic, the highest to nutritional requirement, but growth slower, it is impossible to utilizing the macromolecular substances such as gelatin, starch, growth need to provide paddy Propylhomoserin and methionine, do not carry out nitrate respiration；
DNA sequence is as shown in sequence 12；
Characteristic distributions: summer, North China, deoxidation water pot deoxygenated water.
A13 is He Shi Escherichia (Escherichia hermannii)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 13): white translucent, circular, neat in edge, smooth surface, glossy, easy picking, Soluble in water；
Cell characteristic (B in Figure 13): quarter butt, individualism, does not moves, without spore；
DNA sequence is as shown in sequence 13；
Characteristic distributions: summer, North China, surrounding air.
A14 is bacillus amyloliquefaciens solution starch subspecies (Bacillus amyloliquefaciens subsp. Amyloliquefaciens)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 14): white, in the form of sheets, central authorities' bubbling is glossy, and edge is uneven, flat, unglazed for bacterium colony Pool, thickness, easy picking, soluble in water；
Cell characteristic (B in Figure 14): quarter butt, in pairs；
DNA sequence is as shown in sequence 14；
Characteristic distributions: summer, East China, bottle washer exports.
A15 is bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 15): white, circular, neat in edge, rough surface, there are year colyliform fold projection in central authorities；
Cell characteristic (B in Figure 15): middle bar, in chain, produces spore, does not moves；
Physiological and biochemical property: Gram-positive, KOH is negative；
DNA sequence is as shown in sequence 15；
Characteristic distributions: summer, southeast, filter settles.
A16 is giant cell nitrogen Zymomonas mobilis (Azomonas macrocytogenes)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 16): white is the most faint yellow, circular, neat in edge, smooth surface, glossy, easy picking, Soluble in water；
Cell characteristic (B in Figure 16): bacterium colony outer layer has film, film part cellular morphology to be spherical to thick bar shape, dense arrangement； Inside having liquid, liquid portion cellular morphology is quarter butt, and existence independent paired or in heaps is neither moved, is formed without spore and born of the same parents Capsule；
Physiological and biochemical property: Gram’s staining usually feminine gender, the most variable；Aerobic, but can grow under low oxygen pressure；Almost All bacterial strains produce water solublity or fluorochrome；Chemoheterotrophic bacteria；Sugar, alcohol and organic acid and salt growth can be utilized；Fixed nitrogen, Generally symbiotic nitrogen fixation, does not understands casein, but ammonia salt and some aminoacid can be utilized as nitrogen source；Catalase is positive；
DNA sequence is as shown in sequence 16；
Characteristic distributions: summer, North China, wine filling machine standby pressure carbon dioxide.
A17 is radioprotective acinetobacter calcoaceticus (Acinetobacter radioresistens)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 17): circular, milky, neat in edge, moistening, easy picking, more soluble in water；
Cell characteristic (B in Figure 17): spherical bacterium, grows in pairs, and do not move (being considered as easily coccus), without spore；
Physiological and biochemical property: Gram-negative, without brood cell and flagellum, unpowered, there is pod membrane.Obligate aerobic, nitrate reaction And oxidase test is negative；
DNA sequence is as shown in sequence 17；
Characteristic distributions: summer, North China, Fructus Hordei Germinatus.
A18 is Bacillus subtilis subsp.inaquosorum；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 18): milky, circular, bacterium colony is big, and outer layer has film, inside has thick liquid, and edge is uneven Or the most uneven, mattness, easy picking, it is not readily dissolved in water；
Cell characteristic (B in Figure 18): middle bar, paired or catenation, motion, produce spore；
DNA sequence is as shown in sequence 18；
Characteristic distributions: summer, North China, environment chain road environment fermentation tube sheet environment fermentation tank field.
A19 is bacillus cereus (Bacillus cereus)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 19): white, circular, edge is uneven, and rough surface is matt, and easy picking is soluble in water；
Cell characteristic (B in Figure 19): middle thick bar, paired or independent or chain, motion, produce spore；
Physiological and biochemical property: Gram-positive, KOH is negative；
DNA sequence is as shown in sequence 19；
Characteristic distributions: summer, southeast, the sedimentation of bright beer tank air, filter air, rice wine, bright beer tank sedimentation.
A20 is Klebsiella quasipneumoniae subsp.quasipneumoniae；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 20): milky, circular, neat in edge, it is difficult to picking, glossy, surface wettability, viscous Thick talent for swimming, is not readily dissolved in water；
Cell characteristic (B in Figure 20): short and thick bar, motion, size 0.5～0.8 × 1～2 μm, individually, become double or short chain shape Arrangement.Without brood cell, atrichia, having thicker pod membrane, majority has pili；
Physiological and biochemical property: gram negative bacilli, catalase positive, relatively can send out on Bacterium entericum selective medium Kefir milk sugar, presents coloured bacterium colony；
DNA sequence is as shown in sequence 20；
Characteristic distributions: summer, East China, pack gland rice wine.
A21 is Ludwig enterobacteria (Enterobacter ludwigii)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 21): white, circular, neat in edge, smooth surface, glossy, easy picking, it is soluble in Water；
Cell characteristic (B in Figure 21): quarter butt, individually or in pairs exists, motion；
Physiological and biochemical property: Gram-negative, the KOH wire drawing experiment positive；
DNA sequence is as shown in sequence 21；
Characteristic distributions: summer, North China, canful fermentation liquid.
A22 is staphylococcus xylosus (Staphylococcus xylosus)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 22): circular, milk yellow, neat in edge, center projections, smooth surface, moistening, easily choose Taking, soluble in water, quality is closely knit；
Cell characteristic (B in Figure 22): spherical, does not moves, single growth, 0.8-1.0 μm；
Physiological and biochemical property: gram positive bacteria, KOH wire drawing experiment feminine gender, can grow at anaerobic environment.Catalase sun Property, oxidase negative, fermentation and acid in glucose, produce acid in xylose, arabinose, fructose, mannose, mannitol, half Lactose, sorbitol, salicin, maltose, lactose, trehalose and glycerol, V-P is positive, 7% sodium chloride broth growth, 45 DEG C Growth, gelatinase is positive, alkaline phosphatase positive, the arginine hydrolysis positive.Staphylocoagulase is negative, and the agglomerate factor is cloudy Property, can not ferment melezitose, sucrose, Raffinose, xylitol, cellobiose, nitrate reduction negative, can not be at 10% atmosphere Changing sodium broth growth, urease negative, DNase is negative, dead drunk dirty digestion feminine gender, and lecithinase is negative, Tween 80 hydrolysis feminine gender；
DNA sequence is as shown in sequence 22；
Characteristic distributions: summer, North China, packing shop bottle washer.
A23 is skin spy's acinetobacter calcoaceticus (Acinetobacter pittii)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 23): milky, circular, neat in edge, smooth surface, glossy, thickness, easy picking, Soluble in water；
Cell characteristic (B in Figure 23): club shape, in pairs, does not moves；
Physiological and biochemical property: Gram-negative coccobacillus, oxidase negative, power is negative, nitrate negative；
Characteristic distributions: summer, southeast, rice wine before film.
A24 is roost rose of Sharon pseudomonas (Pseudomonas hibiscicola)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 24): white is slightly turned to be yellow, translucent, circular, neat in edge, smooth surface, glossy, Easily picking, soluble in water；
Cell characteristic (B in Figure 24): quarter butt, 0.5-1 μ m 1.5-4 μm, individually or in pairs exist, motion；
Physiological and biochemical property: Gram-negative, the KOH wire drawing experiment positive, catalase is positive, oxidase positive；Aerobic, change Can heterotrophism.Gelatin can not liquefy；Nitrate can be reduced into nitrite；C.I. 13020., V-P are negative, edwardsiella hoshinae, no Produce hydrogen sulfide, it is impossible to Starch Hydrolysis；Need not somatomedin；The carbon sources such as glucose, lactate, succinate can be utilized；
DNA sequence is as shown in sequence 23；
Characteristic distributions: summer, southeast, the sedimentation of malt workshop air, bottle washer.
A25 is micrococcus lacticus (Micrococcus lactis)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 25): lemon yellow, circular, neat in edge, smooth surface, easy picking, soluble in water；
Cell characteristic (B in Figure 25): coccus, diameter about 1.0 μm, do not move；
Physiological and biochemical property: Gram-positive, does not moves, obligate aerobic, at 10 DEG C to 40 DEG C, then can not give birth to more than 42 DEG C Long, can grow in the range of pH4.5-9.5, optimum pH is 7.5, and the sodium chloride of resistance to 0% to 4% is not given birth at maconkey agar Long, catalase positive, oxidase negative, secrete urase, it is impossible to starch-splitting, Esculin and polysorbas20,40,60 or 80；Lactose, sucrose, cottonseed sugar, Sorbitol, salicin, carubinose, trehalose, d-galactose, n-can not be utilized Acetyl-d-glucosamine, methyl β-d-glucopyranoside or amylopectin；
DNA sequence is as shown in sequence 24；
Characteristic distributions: summer, East China, filling machine surrounding air.
A26 is husky good fortune bacillus cereus (Bacillus safensis)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 26): milky, irregularly, neat in edge, concavity is matt, and marginal swell has light Pool, easy picking, more soluble in water；
Cell characteristic (B in Figure 26): quarter butt, 0.5-0.7 μ m 1.0-1.2 μm individually or in pairs exists, motion；
Physiological and biochemical property: Gram-positive, KOH wire drawing is negative, catalase positive, oxidase positive, resistance to pH5.6 The sodium chloride of 0%-10%, growth temperature 10-50 DEG C, optimum growth temperature 30-37 DEG C, it is impossible to be grown on 4 DEG C or 50 DEG C；
DNA sequence is as shown in sequence 25；
Characteristic distributions: summer, North China, the residual wine of secondary, deoxidation water pot deoxygenated water, environment rice wine tube sheet.
A27 is basophilic Exiguobacterium sp (Exiguobacterium alkaliphilum)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 27): milky, neat in edge, smooth surface, thickness, easy picking, soluble in water；
Cell characteristic (B in Figure 27): gram positive bacteria, amphimicrobian, children's culture in age is brevibacterium, and old culture is Spherical, 1.1～1.2 μ m 1.4～3.2 μm, do not give birth to spore, the most antiacid, move with peritrichous；
Physiological and biochemical property: basophilic, can be in pH6.5～11.5 growth；Chemoheterotrophic bacteria, fermentating metabolism；From glucose, sugarcane Sugar, galactose and some other sugar produce acid；Basophilic, primary product is lactic acid, acetic acid and formic acid；Catalase is positive, oxidase Negative；Reduction nitrate；Gelatin hydrolysate, starch and casein；Optimum growth temperature 37 DEG C；
DNA sequence is as shown in sequence 26；
Characteristic distributions: summer, East China, filling machine surrounding air.
A28 is aerosporus (Bacillus aerophilus)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 28): milk yellow, circular, central authorities' fold, bacterium colony is little, is dried, easy picking, it is more difficult to be dissolved in Water；
Cell characteristic (B in Figure 28): middle bar, motion, amphitrichous；
Physiological and biochemical property: gram positive bacteria, KOH is negative, and catalase is positive, and optimum growth temperature is 30 DEG C；
DNA sequence is as shown in sequence 27；
Characteristic distributions: summer, North China, yeasting air.
A29 is Burkholderia arboris；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 29): yellow green, circular, neat in edge, smooth surface, glossy, easy picking, readily soluble Yu Shui；
Cell characteristic (B in Figure 29): middle bar, individualism, motion, without spore；
Physiological and biochemical property: Gram-negative, the KOH wire drawing experiment positive, oxidase positive, catalase positive, nitric acid Salt is the most Antigen positive hybridomas.Can grow at a temperature of 30 DEG C with 37 DEG C, minority can grow at 42 DEG C.Glucose fermentation, sucrose, wheat Bud sugar, lactose and ribitol produce acid.
DNA sequence is as shown in sequence 28；
Characteristic distributions: summer, southeast, rice wine after film.
A30 is anthrax bacillus (Bacillus anthracis)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 30): white, circular, edge is the most uneven, central authorities' microprotrusion, and mattness is easily chosen Take, soluble in water；
Cell characteristic (B in Figure 30): thick bar, does not moves, and produces spore, catenation；
DNA sequence is as shown in sequence 29；
Characteristic distributions: summer, winter, North China, coarse filtration exports last flushing water, bright beer tank rice wine, bottle washer, does not kills Bacterium free and easy oxygen water pot deoxygenated water, alcoholic air.
A31 is Bacillus tequilensis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 31): white, in the form of sheets, flat, central authorities' white microprotrusion and tarnish, edge uneven and Relatively gloss, and the part between center and edge is glossy, smooth surface, easy picking, soluble in water, produce spore regional centralized, Without the sporangium expanded；
Cell characteristic (B in Figure 31): middle bar, 0.9 μ m 4.0 μm, independent or paired, produce spore, do not move；
Physiological and biochemical property: Gram-positive, KOH wire drawing is negative, catalase positive, oxidase positive, can be in Growing in the range of pH5.5-8.0,25-50 DEG C, fermentation glycerol, xylose, glucose etc. produce acid；
DNA sequence is as shown in sequence 30；
Characteristic distributions: summer, North China, empty bottle, ferment training wind.
A32 is soil bacillus brevis (Brevibacillus agri)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 32): circular, milky, petite, neat in edge, glossy, moistening, soluble in water, Easily picking；
Cell characteristic (B in Figure 32): quarter butt, motion, grow in pairs, raw in spore, do not expand, spore is oval；
Physiological and biochemical property: kOH is negative, Gram-positive, and V-P is negative；Do not hydrolyze starch but liquefaction gelatin；Can be in nutrient meat Soup grows；Glucose produces acid not aerogenesis；
DNA sequence is as shown in sequence 31；
Characteristic distributions: summer, North China, two assure road rice wine.
A33 is Exiguobacterium indicum；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 33): translucent, faint yellow, flat, irregularly, edge is uneven, and smooth surface is easily chosen Take, soluble in water；
Cell characteristic (B in Figure 33): shaft-like, in chain or nodular, does not moves, and children's culture in age is bacillus, old culture For spherical；
Physiological and biochemical property: Gram-positive, KOH wire drawing experiment feminine gender, catalase positive, oxidase positive, growth At 5-30 DEG C and pH value 6-10；It is 7.0 with optimum pH that optimum temperature is 25 DEG C；The normal saline of resistance to 5.8%, it is possible to Salt-free ambient growth；
DNA sequence is as shown in sequence 32；
Characteristic distributions: summer, winter, southeast, filter outlet rice wine, bright beer tank ambient air, bottle washer sink Fall, bottle-washing water.
A34 is Deinococcus ficus；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 34): circular, blush, smooth surface, neat in edge, easy picking, not thickness, readily soluble Yu Shui；
Cell characteristic (B in Figure 34): middle bar, independent or paired, do not move, without spore；
Physiological and biochemical property: gram positive bacteria, KOH tests feminine gender, oxidase positive；UV resistant irradiation (254nm, 8-10 Centimetres 10 minutes), available following compound is as sole carbon source: dextrin, polysorbate40, Tween 80, fructose, maltose, Maltotriose, Sorbitol, stachyose, sucrose, acetic acid, lactic acid, methyl pyruvate, monomethyl succinate, propanoic acid, acetone Acid, succinic acid, alanine etc.；
DNA sequence is as shown in sequence 33；
Characteristic distributions: summer, North China, pack environment air.
A35 is Bacillus siamensis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 35): milky, edge is uneven and in divergent shape, circular, and middle white becomes clear than edge, Easily picking, moist, soluble in water；
Cell characteristic (B in Figure 35): middle stock, motion, independent, paired or bunchiness growth, peritrichous, 0.3-0.6 μ M × 1.5-3.5 μm, elliptic spore is in the middle of thalline or end expands；
Physiological and biochemical property: Gram-positive, KOH is negative, amphimicrobian, and peritrichous can be grown on MacConkey fine jade Fat but cholate medium can not be grown on, catalase positive, oxidase negative, caseinhydrolysate, Esculin, gelatin, DNA And starch；
DNA sequence is as shown in sequence 34；
Characteristic distributions: summer, East China, packing shop compression CO2。
A36 is Xin Shi acinetobacter calcoaceticus (Acinetobacter schindleri)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 36): white translucent, circular, edge is the most uneven, smooth surface, easy picking, is soluble in Water；
Cell characteristic (B in Figure 36): spherical, individually or in pairs exists, does not moves；
Physiological and biochemical property: Gram-negative coccobacillus, oxidase negative, power is negative, nitrate negative Grain-negative ball Bacillus, oxidase negative, power is negative, and nitrate negative, glucose fermentation does not produce acid；
DNA sequence is as shown in sequence 35；
Characteristic distributions: summer, North China, ferment tube sheet.
A37 is Young citric acid bacillus (Citrobacter youngae)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 37): circular, milky, thalline is less, neat in edge, and water moistens, glossy, is soluble in Water, easy picking；
Cell characteristic (B in Figure 37): middle bar, single and paired, diameter about 1.0, long 2.0～6.0 μm, motion, have Peritrichous；
Physiological and biochemical property: gram negative bacteria, KOH tests the positive, oxidase negative, and catalase is positive, can utilize Fructus Citri Limoniae Hydrochlorate is as only carbon source.Do not produce phenylalanine deaminase, gelatinase, lipase and DNA enzymatic.Glucose fermentation produces acid and produces Gas, methyl red test is positive, and VP tests the positive；
DNA sequence is as shown in sequence 36；
Characteristic distributions: summer, North China, bright beer tank surrounding air.
A38 is Deinococcus radioduran；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 38): orange red, circular, neat in edge, smooth surface, glossy, easy picking, readily soluble Yu Shui；
Cell characteristic (B in Figure 38): cell spheroid, diameter 0.5～3.5 μm, paired or tetrad, is big than other coccuses； Do not move, do not produce spore；
Physiological and biochemical property: Gram-positive, but cell wall composition is more complicated, has which floor structure, the change energy of tool respiratory metabolism Heterotrophic bacteria, become inactive tendency to saccharide；Catalase is positive；High resistance gamma-radiation, containing substantial amounts of palmitate.Thermophilic Spend 30～37 DEG C；
Characteristic distributions: summer, North China, environment fine straining.
A39 is Bacillus infantis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 39): pink, irregularly, flat, smooth surface, easy picking, soluble in water；
Cell characteristic (B in Figure 39): middle quarter butt, individualism, does not moves, and produces spore；
Physiological and biochemical property: Gram-positive, KOH string test is negative, catalase positive, and oxidase negative is permissible Utilize galactose, glucose, fructose, mannitol, Sorbitol, aesculin, salicin, maltose, 6-(.alpha.-D-galactosido)-D-glucose., sucrose, Trehalose, Raffinose, starch, glycogen, gluconic acid, cellobiose, lactose and inulin etc., be unfavorable for utilizing glycerol, ribose, Mannose, inositol, xylitol, gentiobiose etc.；
DNA sequence is as shown in sequence 37；
Characteristic distributions: summer, North China, tank fine straining.
A40 is Vietnam's bulkholderia cepasea (Burkholderia vietnamiensis)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 40): milky, circular, less during colony growth 1d, there is microscopic folds on surface, but has light Pool, during more than colony growth 2d, bacterium colony is full, smooth surface, and fold disappears, glossy；
Cell characteristic (B in Figure 40): quarter butt, individually or in pairs exists, and motion, without spore；
Physiological and biochemical property: Gram-negative, the KOH wire drawing experiment positive, chemoheterotrophy, reducible nitrate becomes nitrite； Oxidase positive, arginine dihydrolase is negative；Liquefiable gelatin, edwardsiella hoshinae, do not produce H2S；Acid is produced from glucose； Citrate, adipate, D-alanine, Valine, leucine and ortho-aminobenzoic acid can be utilized, it is impossible to utilize Mus Lee's sugar, maltose, tartrate and erythritol；
DNA sequence is as shown in sequence 38；
Characteristic distributions: summer, North China, residual wine.
A41 is Bacillus aryabhattai；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 41): white, circular, there are a discoid slight convex in central authorities, neat in edge, smooth surface, Glossy, easy picking, soluble in water；
Cell characteristic (B in Figure 41): long thick bar, paired or catenation, do not move, without spore；
DNA sequence is as shown in sequence 39；
Characteristic distributions: winter, North China, alcoholic workshop air, packaging air.
A42 is Enterobacter mori；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 42): white, circular, neat in edge, smooth surface, glossy, easy picking, it is soluble in Water；
Cell characteristic (B in Figure 42): quarter butt, individually or in pairs exists, motion；
DNA sequence is as shown in sequence 40；
Characteristic distributions: winter, southeast, Fructus Hordei Germinatus before pulverizing.
A43 is Cronobacter dublinensis subsp.Lausannensis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 43): milky, circular, neat in edge, smooth surface, bacterium colony is raised slightly, in inside There is an inconspicuous annulus centre, corrugationless, and easy picking is soluble in water；
Cell characteristic (B in Figure 43): quarter butt, motion, without spore；
DNA sequence is as shown in sequence 41；
Characteristic distributions: winter, North China, southeast, the residual wine of bright beer tank ambient air, secondary, pulverize before Fructus Hordei Germinatus, Thick (smart) rice wine of film.
A44 is Pantoea calida；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 44): milky, circular, neat in edge, colony edge is transparent, smooth surface, and water moistens, Glossy, easy picking, soluble in water；
Cell characteristic (B in Figure 44): quarter butt, individually or in pairs exists, and motion, without spore；
DNA sequence is as shown in sequence 42；
Characteristic distributions: winter, southeast, sterilization machine ambient air.
A45 is excrement shortwave Zymomonas mobilis (Brevundimonas faecalis)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 45): pink colour, circular, neat in edge, smooth surface, glossy, easy picking, it is soluble in Water；
Cell characteristic (B in Figure 45): ball, to quarter butt, individually, in pairs or catenation, does not moves, without spore；
DNA sequence is as shown in sequence 43；
Characteristic distributions: winter, North China, between fermented sake ullage.
A46 is staphylococcus saprophyticus (Staphylococcus saprophyticus subsp.Saprophyticus)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 46): milky, circular, petite, neat in edge, smooth surface, glossy, easily choose Take, be not readily dissolved in water；
Cell characteristic (B in Figure 46): coccus, in thyrsiform, does not moves；
DNA sequence is as shown in sequence 44；
Characteristic distributions: winter, North China, sterile wind.
A47 is China Al Kut Salmonella in the last of the ten Heavenly stems (Kurthia huakuii)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 47): white, lamellar, flat, edge is uneven, and smooth surface is glossy, easy picking, Soluble in water；
Cell characteristic (B in Figure 47): white, lamellar, flat, edge is uneven, and smooth surface is glossy, easy picking, Soluble in water；
DNA sequence is as shown in sequence 45；
Characteristic distributions: winter, southeast, Fructus Hordei Germinatus before pulverizing.
A48 is Kurthia gibsonii (Kurthia gibsonii)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 48): lemon yellow, irregularly, flat, edge is uneven, and surface is more smooth, glossy, easily Picking, soluble in water；
Cell characteristic (B in Figure 48): quarter butt, individualism, moves (rotation), without spore；
DNA sequence is as shown in sequence 46；
Characteristic distributions: winter, North China, yeasting air.
A49 is food methanol bacillus cereus (Bacillus methylotrophicus)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 49): white, lamellar, irregularly, edge is uneven, matte surface, matt, easy picking, It is not readily dissolved in water；
Cell characteristic (B in Figure 49): middle stock, long chain, does not moves, has spore；
DNA sequence is as shown in sequence 47；
Characteristic distributions: winter, East China, brewing water.
A50 is Pseudomonas graminis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 50): milky, circular, petite, neat in edge, smooth surface, glossy, easily choose Take, soluble in water；
Cell characteristic (B in Figure 50): middle thick bar, individually or in pairs exists, motion, has spore；
DNA sequence is as shown in sequence 48；
Characteristic distributions: winter, North China, yeasting air.
A51 is simple bacillus cereus (Bacillus simplex)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 51): white, circular, flat, neat in edge, smooth surface, glossy, easy picking, Soluble in water；
Cell characteristic (B in Figure 51): stock, paired or catenation, do not move, without spore；
DNA sequence is as shown in sequence 49；
Characteristic distributions: winter, North China, alcoholic workshop air.
A52 is Lysinibacillus xylanilyticus；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 52): white, flat, irregularly, edge is uneven, and smooth surface is glossy, easy picking, Soluble in water；
Cell characteristic (B in Figure 52): middle stock, does not moves, and single-ended spore also expands, individualism, rare paired existence；
DNA sequence is as shown in sequence 50；
Characteristic distributions: winter, southeast, wine filling machine ambient air.
A53 is Chryseobacterium hispalense；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 53): crocus, circular, neat in edge, smooth surface, water moistens, glossy, easy picking, Soluble in water；
Cell characteristic (B in Figure 53): quarter butt, does not moves, individualism；
DNA sequence is as shown in sequence 51；
Characteristic distributions: winter, East China, rice wine pipeline.
A54 is Kocuria marina；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 54): lemon yellow, circular, bacterium colony is little, neat in edge, and smooth surface is glossy, easily chooses Take, soluble in water；
Cell characteristic (B in Figure 54): coccus, individually or in pairs exists, does not moves, without spore；
Characteristic distributions: winter, southeast, bottle washer, wine filling machine ambient air.
A55 is Pseudomonas kunmingensis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 55): milk yellow, irregularly, edge is uneven dissipates growth, rough surface to surrounding, has light Pool, easy picking is soluble in water；
Cell characteristic (B in Figure 55): quarter butt, individually or in pairs exists, and motion, without spore；
DNA sequence is as shown in sequence 52；
Characteristic distributions: winter, North China, wine filling machine standby pressure carbon dioxide.
A56 is Paenibacillus humicus；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 56): white, circular, neat in edge, smooth surface, water moistens, glossy, inside bacterium colony In muddy sample, easy picking, soluble in water；
Cell characteristic (B in Figure 56): quarter butt, motion, individually, there is spore；
DNA sequence is as shown in sequence 53；
Characteristic distributions: winter, East China, finished wine.
A57 is U.S. bar bacterium (Corynebacterium callunae)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 57): lemon yellow, petite, neat in edge, smooth surface, glossy, easy picking, easily It is dissolved in water；
Cell characteristic (B in Figure 57): ball to quarter butt or bend shaft-like, individually, in pairs or bunchiness arrangement, does not moves, without bud Spore；
DNA sequence is as shown in sequence 54；
Characteristic distributions: winter, North China, alcoholic workshop air.
A58 is Cedecea neteri (Cedecea neteri)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 58): white, irregularly, edge is uneven, and smooth surface is glossy, and easy picking is readily soluble Yu Shui；
Cell characteristic (B in Figure 58): be as short as middle bar, individually or in pairs arrange, moves (moving about), without spore；
DNA sequence is as shown in sequence 55；
Characteristic distributions: winter, North China, sterilization time wine.
A59 is brevibacterium frigoritolerans (Brevibacterium frigoritolerans)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 59): milky, circular, neat in edge, there is circular projection on surface, glossy, easily chooses Take, soluble in water；
Cell characteristic (B in Figure 59): middle stock, chain is rehearsed, is not moved, without spore；
DNA sequence is as shown in sequence 56；
Characteristic distributions: winter, North China, alcoholic workshop air.
A60 is lemon yellow bacillus pumilis (Curtobacterium citreum)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 60): milky white the most faint yellow, circular, neat in edge, smooth surface, glossy, easy picking, Soluble in water；
Cell characteristic (B in Figure 60): quarter butt, individually or in pairs exists, and motion, without spore；
DNA sequence is as shown in sequence 57；
Characteristic distributions: winter, southeast, bright beer tank ambient air.
A61 is Bacillus panaciterrae；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 61): white, in the form of sheets, irregularly, edge is uneven, rough surface, center projections for bacterium colony, There is a fold, easy picking, soluble in water；
Cell characteristic (B in Figure 61): stock, catenation, does not moves, and produces spore；
DNA sequence is as shown in sequence 58；
Characteristic distributions: winter, East China, bottle washer ambient air.
A62:Chryseobacterium hominis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 62): white, circular, petite, neat in edge, smooth surface, glossy, easy picking, It is not readily dissolved in water；
Cell characteristic (B in Figure 62): middle bar, individually or in pairs exists, does not moves, without spore；
DNA sequence is as shown in sequence 59；
Characteristic distributions: winter, North China, pack air.
A63: sphingomonas paucimobilis (Sphingomonas paucimobilis)；
Strain classification: aerobic bacteria；
Condition of culture (A in Figure 63): plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (B in Figure 63): lemon yellow, circular, bacterium colony is little, neat in edge, and smooth surface is glossy, easily chooses Take, soluble in water；
Cell characteristic: quarter butt, individually or in pairs exists, motion；
DNA sequence is as shown in sequence 60；
Characteristic distributions: winter, East China, finished wine.
A64 is Erwinia tasmaniensis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 64): yellow is translucent, early growth period is faint yellow, flat, and there are a year colyliform depression in central authorities, Neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic (B in Figure 64): quarter butt, individually or in pairs exists, does not moves；
DNA sequence is as shown in sequence 61；
Characteristic distributions: winter, southeast, Fructus Hordei Germinatus before pulverizing.
A65 is Pseudomonas aeruginosa (Pseudomonas aeruginosa)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 65): milk yellow, translucent, lamellar, irregularly, edge is uneven, and smooth surface has light Pool, under reflection light irradiates, periphery of bacterial colonies is blueness, and chromogenic element, culture medium darkens, easy picking, soluble in water；
Cell characteristic (B in Figure 65): quarter butt, individually or in pairs exists, and motion, without spore；
DNA sequence is as shown in sequence 62；
Characteristic distributions: winter, southeast, brewing water.
A66 is pseudo-blood Sphingol single-cell (Sphingomonas pseudosanguinis)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 66): yellow, circular, neat in edge, smooth surface, glossy, easy picking, it is soluble in Water；
Cell characteristic (B in Figure 66): be as short as middle bar, individualism, motion
DNA sequence is as shown in sequence 63；
Characteristic distributions: winter, southeast, the residual wine of secondary.
A67 is nicotinic acid bacillus cereus (Bacillus niacini)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 67): white, irregularly, neat in edge, smooth surface, matt, easy picking, readily soluble Yu Shui；
Cell characteristic (B in Figure 67): middle stock, individually or in pairs exists, does not moves；
DNA sequence is as shown in sequence 64；
Characteristic distributions: winter, North China, alcoholic workshop air.
A68 is acetyl Exiguobacterium sp (Exiguobacterium acetylicum)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 68): translucent, circular, neat in edge, smooth surface, glossy, easy picking, readily soluble Yu Shui；
Cell characteristic (B in Figure 68): short and thick to long thick bar or bending, paired or catenation, do not move, without spore；
DNA sequence is as shown in sequence 65；
Characteristic distributions: winter, North China, pack air.
A69 is Deinococcus caeni；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 69): crocus, circular, more flat, neat in edge, there is slight fold on surface, relatively has light Pool, easy picking, soluble in water；
Cell characteristic (B in Figure 69): middle thick bar, independent, paired or catenation, do not move；
DNA sequence is as shown in sequence 66；
Characteristic distributions: winter, North China, packing shop surrounding air.
A70 is Deinococcus grandis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 70): crocus, circular, neat in edge, smooth surface, glossy, easy picking, readily soluble Yu Shui；
Cell characteristic (B in Figure 70): short and thick bar, individually or in pairs exists, does not moves；
DNA sequence is as shown in sequence 67；
Characteristic distributions: winter, southeast, filter outlet rice wine.
A71 is plant bacillus pumilis (Curtobacterium plantarum)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 71): faint yellow, translucent, circular, neat in edge, smooth surface, glossy, easily choose Take, soluble in water；
Cell characteristic (B in Figure 71): quarter butt, individually or in pairs exists, and motion, without spore；
DNA sequence is as shown in sequence 68；
Characteristic distributions: winter, East China, Fructus Hordei Germinatus, wine filling machine ambient air before finished product rice wine, pulverizing.
B1 is Leuconostoc mesenteroides dextran subspecies (Leuconostoc mesenteroides subsp.dextranicun)；
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics (A in Figure 72): circular, milky, smooth surface, neat in edge, easy picking, soluble in water；
Cell characteristic (B in Figure 72): quarter butt, exists in pairs, and cell size is 0.5～0.7 × 0.7～1.2mm, does not moves, Without spore；
Physiological and biochemical property: gram positive bacteria, KOH is negative, and catalase is negative, glucose fermentation, fructose, lactose, half Lactose, sucrose, maltose, mannose, trehalose, do not hydrolyze arginine；Growth temperature range 2 DEG C-53 DEG C, the most suitable growth Temperature 3O DEG C-40 DEG C；Acid resistance is strong, and growth optimum pH is 5.5～6.2, can grow in the environment of pH≤5, and in Property or initial alkalescence under the conditions of growth rate reduce；
DNA sequence is as shown in sequence 69；
Characteristic distributions: summer, North China, pack bottle washer surrounding air.
B2 is Lactobacillus brevis (lactobacillus brevis)；
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics (A in Figure 73): circular, milky, edge is uneven, rough surface, moistening, and easy picking is soluble in Water；
Cell characteristic (B in Figure 73): middle stock, does not moves, single growth；
Physiological and biochemical property: Gram-positive, KOH is negative, and negative catalase can utilize fructose, arabinose, honey Disaccharide, xylose produce acid；
DNA sequence is as shown in sequence 70；
Characteristic distributions: summer, winter, North China, air, dilution after rice wine, pipeline rice wine, coarse filtration machine outlet rice wine, Yeasting air, C0 enter bright beer tank rice wine, bright beer tank rice wine for after yeast height bubble phase, chain of packages road surrounding air, dilution.
B3 is Lactococcus garvieae (Lactococcus garvieae)；
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics (A in Figure 74): milky, circular, smooth surface, easy picking, soluble in water, thalline is less；
Cell characteristic (B in Figure 74): quarter butt, do not move；
Physiological and biochemical property: without spore, G+ coccus, bacterium colony smooth surface is glossy, low projection, edge is complete, and thickness is yellowish The opaque circular colonies of color；Thalline is ellipsoid, in pairs, becomes chain alignment；Acid not aerogenesis can be produced, it is impossible to utilize starch from glucose, Milk acidification can be made；Resistance to 4%NaCl；
DNA sequence is as shown in sequence 71；
Characteristic distributions: filling machine in winter in summer standby pressure CO2。
B4 is Lactobacillus paracasei subsp.tolerans；
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics (A in 75): circular, white, smooth surface, neat in edge, easy picking, soluble in water；
Cell characteristic (B in 75): middle stock, bending, individually grow, do not move；
Physiological and biochemical property: can be 10 DEG C and 37 DEG C growths, 40 DEG C then can not grow, 72 DEG C, 40s still can survive, fermentation produce Lactic acid, glucose fermentation, fructose, lactose, mannose, n acetylglucosamine n etc. produce acid.Urease negative, does not hydrolyze Esculin；
DNA sequence is as shown in sequence 72；
Characteristic distributions: summer, North China, pipeline rice wine.
B5 is cibarium Wei Si Salmonella (Weissella cibaria)；
Strain classification: anaerobic bacteria；
Condition of culture: MRS agar culture medium, cultivates 5 days for 28 DEG C；
Colony characteristics (A in Figure 76): white, circular, bacterium colony is little, smooth surface, neat in edge；
Cell characteristic (B in Figure 76): quarter butt, does not moves, individually or in pairs exists；
Physiological and biochemical property: gram positive bacteria, is formed without spore, does not moves, and does not utilize rhamnose, can utilize most of carbon Source, utilizes arginine, and decomposition glucose produces CO2, acidproof, optimum pH 5.5-6.2；
DNA sequence is as shown in sequence 73；
Characteristic distributions: summer, southeast, bright beer tank air.
B6 is Pediococcus claussenii；
Strain classification: amphimicrobian；
Condition of culture: MRS agar culture medium, cultivates 5d for 28 DEG C；
Colony characteristics (A in Figure 77): milky, circle, neat in edge, picking soluble in water, easy；
Cell characteristic (B in Figure 77): spherical, do not move, diameter 1.2～2.0 μm；
DNA sequence is as shown in sequence 74；
Characteristic distributions: summer, East China, canful rice wine.
B7 is Pediococcus lolii；
Strain classification: anaerobic bacteria
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C
Colony characteristics (A in Figure 78): milky, circular, neat in edge, smooth surface, glossy, easy picking, readily soluble Yu Shui
Cell characteristic (B in Figure 78): coccus, independent, paired or tetrad arrangement, do not move
DNA sequence is as shown in sequence 75；
Characteristic distributions: summer, southeast, wine filling machine water.
B8 is leuconostoc lactis (Leuconostoc holzapfelii)；
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics (A in Figure 79): milky, circular, neat in edge, smooth surface, glossy, easy picking, readily soluble Yu Shui；
Cell characteristic (B in Figure 79): coccus, in catenation, does not moves；
Physiological and biochemical property: Gram-positive, KOH wire drawing experiment feminine gender, catalase is negative, glucose fermentation, fructose, breast Sugar, sucrose, maltose, galactose；Azymic cellobiose, xylose；Do not hydrolyze arginine, amphimicrobian；
DNA sequence is as shown in sequence 76；
Characteristic distributions: summer, North China, Fructus Hordei Germinatus before pulverizing.
B9 is Lactococcus lactis subsp.tructae；
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics (A in Figure 80): white, translucent, circular, neat in edge, smooth surface, glossy, easy picking, Soluble in water；
Cell characteristic (B in Figure 80): coccus, 0.5-1.2 μ m 0.5-1.5 μm is catenation, does not moves；
Physiological and biochemical property: Gram-positive, amphimicrobian, chemoheterotrophy, fermentating metabolism, with some carbohydrate fermentation Produce acid, based on L (+)-lactic acid, not aerogenesis.Nutritional requirement is complicated, and catalase is negative, oxidase negative；Optimum temperature 30 DEG C, The sodium chloride of resistance to 4%, fermentating maltose, lactose, ribose, sucrose, mannitol etc. produce acid；
DNA sequence is as shown in sequence 77；
Characteristic distributions: summer, North China, environment coarse filtration.
B10 is lactococcus lactis subsp.cremoris (Lactococcus lactis subsp.cremoris)；
Strain classification: amphimicrobian；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics (A in Figure 81): circular, petite, white, smooth surface, neat in edge, easy picking, not thickness, Soluble in water；
Cell characteristic (B in Figure 81): coccus, paired or bunchiness, 0.5～1.2 μ m 0.5～1.5 μm, do not move, no Sprout spore, without pod membrane；
Physiological and biochemical property: gram positive bacteria, KOH tests feminine gender, and catalase is negative, oxidase negative, chemoheterotrophy, Fermentating metabolism, with some carbohydrate fermentation produce acid, based on L (+)-lactic acid, not aerogenesis.Nutritional requirement is complicated,.The suitableeest Temperature 30 DEG C, can be 10 DEG C of growths, it is impossible to grow under the conditions of 45 DEG C, be weak to the NaCl of 4%, can utilize glucose, gala Sugar, maltose and mannitol, it is impossible to utilize inulin, produce extracellular polysaccharide.
Characteristic distributions: summer, North China, Wort oxygenating sterile wind.
B11 is Pediococcus pentosaceus (Pediococcus pentosaceus)；
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics (A in Figure 82): circular, white, neat in edge, smooth surface, moistening, easy picking, soluble in water；
Cell characteristic (B in Figure 82): coccus, tetrad or in thyrsiform；
Physiological and biochemical property: Gram-positive, KOH feminine gender optimum growth temperature 35 DEG C, maximum growth temperature 42-45 DEG C；
DNA sequence is as shown in sequence 78；
Characteristic distributions: summer, North China, Fructus Hordei Germinatus before pulverizing.
B12 is Lactobacillus plantarum plant subspecies (Lactobacillus plantarum subsp.plantarum)
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics (A in Figure 83): circular, milky, bacterium colony is full, smooth surface, neat in edge, easy picking, easily It is dissolved in water；
Cell characteristic (B in Figure 83): straight or curved is shaft-like, single, sometimes paired or one-tenth chain, does not moves；
Physiological and biochemical property: gram positive bacteria, KOH tests feminine gender, negative catalase, and optimum growth temperature is 30～35, Optimum pH about 6.5, belongs to homofermentative lactic bacteria；
DNA sequence is as shown in sequence 79；
Characteristic distributions: summer, North China, yeasting air, coarse filtration machine export last flushing water, C0 for yeast height bubble the phase, Fermentation deoxygenated water.
B13 is leuconostoc pseudomesenteroides (Leuconostoc pseudomesenteroides)；
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics (A in Figure 84): white, micro-Huang, circular, neat in edge, smooth surface, glossy, easy picking, Soluble in water；
Cell characteristic (B in Figure 84): quarter butt, individually or in pairs exists, does not moves, without spore；
DNA sequence is as shown in sequence 80；
Characteristic distributions: winter, North China, fine straining outlet wine liquid.
B14 is Clostridium beijerinckii (Clostridium beijerinckii)；
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics (A in Figure 85): white, irregularly, edge is uneven, and smooth surface is glossy, and easy picking is readily soluble Yu Shui；
Cell characteristic (B in Figure 85): stock, the long thick bar of minority, individually or in pairs exist, move (moving about), without spore；
DNA sequence is as shown in sequence 81；
Characteristic distributions: winter, North China, fine straining outlet wine liquid.
B15 is for solving ornithine Raoul bacterium (Raoultella ornithinolytica)；
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics (A in Figure 86): white, translucent, circular, neat in edge, smooth surface, glossy, easy picking, Soluble in water；
Cell characteristic (B in Figure 86): middle thick bar, individualism, does not moves, without spore；
DNA sequence is as shown in sequence 82；
Characteristic distributions: winter, North China, pack rice wine pipeline rice wine.
C1 is Aureobasidium；
Strain classification: fungus；
Condition of culture: potato dextrose agar, cultivates 7 days for 27 DEG C；
Colony characteristics (A in Figure 87): white, irregular or circular, neat in edge, smooth surface, easy picking, it is soluble in Water；
Cell characteristic (B in Figure 87): cell ovalize two ends are the sharpest；
DNA sequence is as shown in sequence 83；
Characteristic distributions: winter, southeast, fermentation plant surrounding air.
C2 is Rhodotorula mucilaginosa；
Strain classification: fungus；
Condition of culture: potato dextrose agar, cultivates 7 days for 27 DEG C；
Colony characteristics (A in Figure 88): 7d, 13mm, milky, circular, neat in edge, water moistens, and smooth surface has light Pool, quality paste, easy picking, it is not readily dissolved in water (yeast)；
Cell characteristic (B in Figure 88): circular thalline, single-ended budding；
DNA sequence is as shown in sequence 84；
Characteristic distributions: winter, Shenyang, environment rice wine tube sheet.
C3 is Cryptococcus rajasthanensis；
Strain classification: fungus；
Condition of culture: potato dextrose agar, cultivates 7 days for 27 DEG C；
Colony characteristics (A in Figure 89): 7d, 10mm, red, irregularly, edge is uneven, and there is fold on surface, matt；
Cell characteristic (B in Figure 89): yeast, thalline is oval to circular, without false silk
DNA sequence is as shown in sequence 85；
Characteristic distributions: winter, North China, bottle cap.
The probability of happening of microorganism be season in summer in winter two North China, East China, each stage in the southeast and the Northwest (Figure 90) production process The accounting of main contaminated bacteria.Wherein each stage in production process is the production process having occurred to pollute, and is not whole operation. North China includes Beijing, Inner Mongolia Autonomous Region, Hebei province, Shanxi Province, Shandong Province, Liaoning Province, Heilongjiang Province and Jilin Save；East China include Fujian Province, Zhejiang Province, Hunan Province, Hubei Province, Jiangxi Province, Henan Province, Anhui Province, Jiangsu Province and Shanghai City；Southeast includes Guangxi Zhuang Autonomous Region, Guangdong Province, Yunnan Province, Guizhou Province, Sichuan Province and Chongqing City；Northwest Area includes Xinjiang Uygur Autonomous Regions, Tibet Autonomous Region, Ningxia Hui Autonomous Region, Qinghai Province, Gansu Province and Shaanxi Province.
The probability of happening of microorganism specifically includes:
Summer over North China aerobic bacteria is at each production process probability of happening:
Top box or galenic bottle cap: bacillus subtilis 11%, pantoea agglomerans 55%, Soil Bacillus 13%；
Dilution deoxidation water pot: acinetobacter calcoaceticus 25%；
Dilution oxygen remove exports deoxygenated water: Bacillus licheniformis 40%, Bacillus pumilus 17.5%, plants raw Raoul bacterium 17.3%；
The strainer last flushing water of outlet: bacillus amyloliquefaciens 50%, Bacillus coagulans 45%；
Coarse filtration machine outlet rice wine liquid: bacillus subtilis 38%；
Storage wine phase fermentation liquid: bacillus amyloliquefaciens 20%, Acetobacter pasteurianus 15%；
High bubble phase fermentation liquid: bacillus amyloliquefaciens 36%；
Canful fermentation liquid: bacillus amyloliquefaciens 35%, bacillus subtilis 19%；
Softening water (brewing water): Bacillus coagulans 30%, streptococcus thermophilus 7%.
Summer over North China anaerobe is at each production process probability of happening:
Rice wine after gland: zymomonas mobilis 45%；
Rice wine pipeline: Lin Denaishi lactobacillus 23%, Lactobacillus brevis 20%, Lactobacillus buchneri 10%；
The residual wine of secondary: zymomonas mobilis 28%, Lactobacillus brevis 23%；
Bright beer tank rice wine liquid is entered: Lin Denaishi lactobacillus 46%, pantoea agglomerans 45%, Lactobacillus buchneri 15% after dilution；
Storage wine phase fermentation liquid: zymomonas mobilis 6%, Lin Denaishi lactobacillus 10%；
Canful fermentation liquid: zymomonas mobilis 8%, Pectinatus 23%；
Softening water: leukonid 10%.
North China aerobic bacteria in winter is at each production process probability of happening:
Rice wine after film: plant raw Raoul bacterium 66%；
Sharp foam water: Bacillus coagulans 9%；
The residual wine of secondary: Bacillus coagulans 83%；
Dilution oxygen remove exports deoxygenated water: Bacillus coagulans 7%；
The strainer last flushing water of outlet: Bacillus coagulans 33%；
The coarse filtration machine last flushing water of outlet: acinetobacter calcoaceticus 20%；
The filter last flushing water of outlet: Bacillus coagulans 33%；
CIP hot-water cylinder hot water: Bacillus coagulans 1%, bacillus amyloliquefaciens 11%, Bacillus pumilus 1%；
Softening water (brewing water): acetobacter aceti 6%.
North China's anaerobe in winter is at each production process probability of happening:
Rice wine after film: Lin Denaishi lactobacillus 26%；
Wine filling machine standby pressure carbon dioxide, compressed air: Pectinatus 13%；
The residual wine of secondary: Lin Denaishi lactobacillus 55%；
Canful fermentation liquid: Lactobacillus brevis 14%；
Co is for yeast spread cultivation liquid: Pectinatus 20%.
East China aerobic bacteria in summer is at each production process probability of happening:
Rice wine after gland: pantoea agglomerans 20%；
Rice wine pipeline: pantoea agglomerans 54%, bacillus amyloliquefaciens 32%, Acetobacter pasteurianus 24%；
The residual wine of secondary: streptococcus thermophilus 24%；
Bright beer tank rice wine liquid is entered: plant raw Raoul bacterium 36% after dilution；
Canful fermentation liquid: pantoea agglomerans 10%, Acetobacter pasteurianus 6%, Bacillus coagulans 6%；
Soften water: plant raw Raoul bacterium 32%.
East China anaerobe in summer is at each production process probability of happening:
Wine filling machine standby pressure carbon dioxide, compressed air: zymomonas mobilis 36.25%；
The residual wine of secondary: Pediococcus pentosaceus 12%, mound shape lactobacillus 10%；
Bright beer tank rice wine liquid is entered: zymomonas mobilis 57%, Lactobacillus brevis 26% after dilution；
Filter outlet rice wine liquid: Lactobacillus brevis 4%, pantoea agglomerans 17%；
Storage wine phase fermentation liquid: zymomonas mobilis 44%, Lactobacillus brevis 19%, Lactobacillus buchneri 5%；
High bubble phase fermentation liquid: zymomonas mobilis 5%, harmful sheet coccus 25%, lactobacillus casei 9%；
Canful fermentation liquid: Lactobacillus brevis 30%.
East China aerobic bacteria in winter is at each production process probability of happening:
High bubble phase fermentation liquid: plant raw Raoul bacterium 76%, Bacillus pumilus 2%；
Canful fermentation liquid: Acetobacter pasteurianus 88%, Bacillus circulans 6%；
CIP hot-water cylinder hot water: Bacillus circulans 34%；
Aseptic water pot: bacillus subtilis 25%.
East China anaerobe in winter is at each production process probability of happening:
Rice wine pipeline: Lactobacillus brevis 5%, lactobacillus casei 18%；
The residual wine of secondary: Lin Denaishi lactobacillus 23%, Lactobacillus buchneri 35%；
Canful fermentation liquid: lactobacillus casei 59%, Lin Denaishi lactobacillus 19%；
Aseptic water pot: Lactobacillus brevis 83%.
Southeast aerobic bacteria in summer is at each production process probability of happening:
Rice wine before film: acinetobacter calcoaceticus 36%；
Top box or galenic bottle cap: Bacillus pumilus 13%, Acetobacter pasteurianus 13%, pseudomonas 6%；
Sharp foam water: Bacillus licheniformis 26%；
The residual wine of secondary: Bacillus licheniformis 7%, streptococcus thermophilus 36%, Bacillus pumilus 16%；
Bright beer tank rice wine liquid is entered: Bacillus licheniformis 20%, bacillus subtilis 23%, chryseobacterium indologenes 6% after dilution；
Softening water: Bacillus coagulans 70%, acinetobacter calcoaceticus 13%, chryseobacterium indologenes 10%.
Southeast anaerobe in summer is at each production process probability of happening:
The residual wine of secondary: Lactobacillus brevis 69%, Wei Si Salmonella 3%, Lin Denaishi lactobacillus 9%；
Bright beer tank rice wine liquid is entered: Lactobacillus brevis 59% after dilution；
Storage wine phase fermentation liquid: zymomonas mobilis 13%；
High bubble phase fermentation liquid: lactobacillus casei 10%.
Southeast aerobic bacteria in winter is at each production process probability of happening:
Finished wine: Bacillus coagulans 16%, plants raw Raoul bacterium 8%；
Sharp foam water: acetobacter aceti 72%；
The residual wine of secondary: bacillus amyloliquefaciens 80%, scytoblastema noy bacillus cereus 5%；
Aseptic water pot: bacillus subtilis 24%.
Southeast anaerobe in winter is at each production process probability of happening:
Finished wine: Lactobacillus brevis 19%, Lactobacillus plantarum 19%；
Rice wine after gland: Lactobacillus brevis 25%, Lactobacillus buchneri 25%；
The residual wine of secondary: megacoccus 56%, harmful sheet coccus 38%；
Bright beer tank rice wine liquid is entered: Lactobacillus brevis 63%, lactobacillus casei 13%, pantoea agglomerans 13% after dilution；
High bubble phase fermentation liquid: zymomonas mobilis 38%.
The Northwest's Summer and winter aerobic bacteria is at each production process probability of happening:
Bottle washer outlet empty bottle: Bacillus licheniformis 67%, Edwardsiella tarda 8%, bacillus cereus 7%；
Bright beer tank rice wine liquid is entered: Bacillus coagulans 25%, streptococcus thermophilus 45% after dilution；
Dilution oxygen remove exports deoxygenated water: Bacillus coagulans 5%, plants raw Raoul bacterium 40%；
Strainer outlet rice wine liquid: Bacillus licheniformis 72%；
CIP hot-water cylinder hot water: Bacillus coagulans 40%；
Softening water: Bacillus licheniformis 34%.
The Northwest's Summer and winter anaerobe is at each production process probability of happening:
Bottle washer outlet empty bottle: pantoea agglomerans 76%；
Bright beer tank rice wine liquid is entered: Lin Denaishi lactobacillus 32% after dilution；
Strainer outlet rice wine liquid: Lin Denaishi lactobacillus 49%；
Bright beer tank rice wine liquid is entered: Lin Denaishi lactobacillus 32% after dilution；
Strainer outlet rice wine liquid: Lin Denaishi lactobacillus 49%.
The qualification of contaminating microorganisms in embodiment 2, beer production
Gather company of Chifeng of Yanjing Brewery packing shop bottle washer ambient air and carry out microorganism culturing, it is thus achieved that microorganism culturing Thing.Culture of microorganism is carried out isolated and purified, obtains the pure culture of microorganism, take the pure culture of a kind of microorganism according to Following method is identified.
1, morphological characteristic observation and the physiological and biochemical property of microorganism pure culture is identified
Continue microorganism pure culture to cultivate in plate count agar culture medium, observe and record the shape of microorganism pure culture State feature, carries out physiological and biochemical property qualification to microorganism pure culture simultaneously.
Result shows: the colony characteristics (Fig. 1) of microorganism pure culture is circle, milky, smooth surface, neat in edge, Easily picking, soluble in water.Cell is rod-short, exists the most in pairs, and cell size is 0.5～0.7 × 0.7～1.2mm, no Motion, without spore.Gram positive bacteria, KOH enzyme is negative, and catalase is negative, glucose fermentation, fructose, lactose, galactose, Sucrose, maltose, mannose, trehalose, do not hydrolyze arginine；Growth temperature range 2 DEG C～53 DEG C, optimum growth temperature 3O DEG C～ 40℃；Acid resistance is strong, and growth optimum pH is 5.5～6.2, can grow in the environment of pH≤5, and neutral or initially alkaline Under the conditions of growth rate reduce.
The above results is compared with the bio information that the module in system in embodiment 1 is carried, tentatively judges that it is Lactobacillus paracasei subsp.tolerans。
2, the molecular biology identification of microorganism pure culture
The genomic DNA of the microorganism pure culture of extraction separation and purification.With the genomic DNA of microorganism pure culture as template, Utilize universal primer 27F and 1492R to carry out PCR amplification, obtain pcr amplification product.The sequence of universal primer 27F and 1492R is as follows:
27F:5 '-AGAGTTTGATCCTGGCTCAG-3 '；
1492R:5 '-GGTTACCTTGTTACGACTT-3 '.
Wherein, 50 μ L PCR reaction systems are by 5 μ L 10 × buffer, 1uL dNTPs (10 μm ol/ml), 1 μ L forward primer 27F (10 μm ol/100uL), 1 μ L downstream primer 1492R (10 μm ol/100uL), 3 μ L DNA profilings, 0.5 μ L Taq Polymerase and 38.5 μ L ddH2O forms.
PCR reaction condition is: 94 DEG C of denaturations 3min；94 DEG C of 1min, 55 DEG C of 1min, 72 DEG C of 2min, 30 circulations；72℃ Extend 10min.
PCR primer being checked order, two-way survey is led to, and is spliced by sequencing result, obtains the DNA information of microorganism pure culture. The sequencing result of acquisition is compared with the bio information that the module in system in embodiment 1 is carried.
Result shows: the genomic dna sequence of microorganism pure culture and the Lactobacillus of system in embodiment 1 The homogeneity of paracasei subsp.tolerans is 100%.
Show according to sequence alignment result, separate from company of Chifeng of Yanjing Brewery packing shop bottle washer ambient air and obtain Microorganism pure culture be Lactobacillus paracasei subsp.tolerans, it easily pollutes rice wine pipeline and film mistake Rice wine after filter, easily detects in Chifeng area, North China.

Claims (10)

1. identify or the method for auxiliary qualification brewery sludge, comprise the steps:
(1) microorganism in isolated and purified sample, obtains bacterial strain；
(2) morphological characteristic and the physiological and biochemical property of described bacterial strain are detected；
(3) extract the genomic DNA of described bacterial strain, obtain conserved DNA sequences；
(4) morphological characteristic of described bacterial strain, physiological and biochemical property and described conserved DNA sequences are appointed in claim 4-9 The microbial information that data base described in system described in records is compared, and determines whether described bacterial strain is brewery sludge, as The most described bacterial strain is contaminated bacteria, determines the kind name of described bacterial strain；
Any link of beer production taken from by described sample.
Method the most according to claim 1, it is characterised in that: described step (4) also includes to obtain in step (3) Described bacterial strain genomic DNA and claim 4-9 in the beer production described in arbitrary described system pollutes micro-life The DNA sample of thing hybridizes.
Method the most according to claim 1 and 2, it is characterised in that: described step (4) also includes according to comparison result Determine the probability of occurrence of described bacterial strain.
4. identify or a system for auxiliary qualification medicated beer microorganism, including the DNA sample of contaminating microorganisms in beer production With the data base recording described contaminating microorganisms information；Described contaminating microorganisms information includes 1)-4) at least one:
1) conserved DNA sequences of described contaminating microorganisms；
2) morphological feature of described contaminating microorganisms；
3) physiological and biochemical property of described contaminating microorganisms；
4) the difference feature of described contaminating microorganisms.
System the most according to claim 4, it is characterised in that: described in described data base, contaminating microorganisms includes 71 strains Aerobic bacteria, 15 strain anaerobic bacterias and 3 fungal strains.
System the most according to claim 5, it is characterised in that: described 71 strain aerobic bacteria include: e coil k 1 pneumonia Bacterium ozena subspecies (Klebsiella pneumoniae subsp.ozaenae), Serratia marcescens subsp. Sakuensis、Aquitalea magnusonii、Chryseobacterium arthrosphaerae、Enterobacter Xiangfangensis, Macrococcus equipercicus, Ralstonia insidiosa, enterobacter asburiae (Enterobacter asburiae), Acinetobacter baumannii (Acinetobacter baumannii), Acinetobacter Nosocomialis, chryseobacterium indologenes (Chryseobacterium indologenes), Xanthomonas sacchari, He Shi Escherichia (Escherichia hermannii), bacillus amyloliquefaciens solution starch subspecies (Bacillus Amyloliquefaciens subsp.Amyloliquefaciens), bacillus amyloliquefaciens plant subspecies (Bacillus Amyloliquefaciens subsp.plantarum), giant cell nitrogen Zymomonas mobilis (Azomonas macrocytogenes), anti-spoke Penetrate acinetobacter calcoaceticus (Acinetobacter radioresistens), Bacillus subtilis subsp.inaquosorum, Bacillus cereus (Bacillus cereus), Klebsiella quasipneumoniae subsp.quasipneumoniae, Ludwig enterobacteria (Enterobacter ludwigii), staphylococcus xylosus (Staphylococcus xylosus), skin Special acinetobacter calcoaceticus (Acinetobacter pittii), roost rose of Sharon pseudomonas (Pseudomonas hibiscicola), breast are micro- Coccus (Micrococcus lactis), husky good fortune bacillus cereus (Bacillus safensis), basophilic Exiguobacterium sp (Exiguobacterium alkaliphilum), aerosporus (Bacillus aerophilus), Burkholderia Arboris, anthrax bacillus (Bacillus anthracis), Bacillus tequilensis, soil bacillus brevis (Brevibacillus agri)、Exiguobacterium indicum、Deinococcus ficus、Bacillus Siamensis, Xin Shi acinetobacter calcoaceticus (Acinetobacter schindleri), Young citric acid bacillus (Citrobacter Youngae), Deinococcus radioduran, Bacillus infantis, Vietnam bulkholderia cepasea (Burkholderia vietnamiensis)、Bacillus aryabhattai、Enterobacter mori、Cronobacter dublinensis Subsp.Lausannensis, Pantoea calida, excrement shortwave Zymomonas mobilis (Brevundimonas faecalis), saprophytic Staphylococcus (Staphylococcus saprophyticus subsp.Saprophyticus), China Al Kut Salmonella (Kurthia in the last of the ten Heavenly stems Huakuii), Kurthia gibsonii (Kurthia gibsonii), food methanol bacillus cereus (Bacillus Methylotrophicus), Pseudomonas graminis, simple bacillus cereus (Bacillus simplex), Lysinibacillus xylanilyticus、Chryseobacterium hispalense、Kocuria marina、 Pseudomonas kunmingensis, Paenibacillus humicus, U.S. bar bacterium (Corynebacterium Callunae), Cedecea neteri (Cedecea neteri), brevibacterium frigoritolerans (Brevibacterium Frigoritolerans), lemon yellow bacillus pumilis (Curtobacterium citreum), Bacillus panaciterrae, Chryseobacterium hominis, sphingomonas paucimobilis (Sphingomonas paucimobilis), Erwinia Tasmaniensis, Pseudomonas aeruginosa (Pseudomonas aeruginosa), pseudo-blood Sphingol single-cell (Sphingomonas Pseudosanguinis), nicotinic acid bacillus cereus (Bacillus niacini), acetyl Exiguobacterium sp (Exiguobacterium Acetylicum), Deinococcus caeni, Deinococcus grandis, plant bacillus pumilis (Curtobacterium plantarum)；
Described 15 strain anaerobic bacterias include: Leuconostoc mesenteroides dextran subspecies (Leuconostoc mesenteroides Subsp.dextranicun), Lactobacillus brevis (Lactobacillus brevis), Lactococcus garvieae (Lactococcus Garvieae), Lactobacillus paracasei subsp.tolerans, cibarium Wei Si Salmonella (Weissella cibaria), Pediococcus claussenii, Pediococcus lolii, leuconostoc lactis (Leuconostoc holzapfelii), Lactococcus lactis subsp.tructae, lactococcus lactis subsp.cremoris (Lactococcus lactis Subsp.cremoris), Pediococcus pentosaceus (Pediococcus pentosaceus), Lactobacillus plantarum (Lactobacillus Plantarum subsp.plantarum), leuconostoc pseudomesenteroides (Leuconostoc pseudomesenteroides), visit Family name clostridium (Clostridium beijerinckii), solution ornithine Raoul bacterium (Raoultella ornithinolytica)；
Described 3 fungal strains include: Aureobasidium, rhodotorula mucilaginosa (Rhodotorula mucilaginosa), Cryptococcus rajasthanensis。
7. according to the system described in claim 5 or 6, it is characterised in that: described aerobic bacteria and described anaerobic bacteria conservative DNA sequence is 16s rDNA sequence；The conserved DNA sequences of described fungus is ITS sequence.
8. according to the arbitrary described system of claim 4-7, it is characterised in that: described pollution described in described data base is micro- Bio information is as shown in A1-A71, B1-B15 and C1-C3；A1-A71 is the information of described 71 kinds of aerobic bacteria；B1-B15 is The information of described 15 kinds of anaerobic bacterias；C1-C3 is the information of described 3 kinds of funguses；
A1 is Klebsiella pneumonia ozena subspecies (Klebsiella pneumoniae subsp.ozaenae)；
Strain classification: aerobic bacteria；
Condition of culture: plate count culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: milky, circular, neat in edge, surface is glossy, moistening, easily provokes, soluble in water；
Cell characteristic: quarter butt, size 0.5～0.8 μ m 1～2 μm, individually, become double or short catenation；Without brood cell without whip Hair, does not moves, has thicker pod membrane, and majority has pili；
Physiological and biochemical property: Gram-negative, KOH wire drawing is positive, produces the 2,3-butanediol main end as glucose fermentation End product, VP tests the positive；Form few lactic acid, acetic acid and formic acid with mixed acid fermentation, form many ethanol；Fermentation inositol, Hydrolysis carbamide, does not produce ODC Ornithine decarboxylase or H2S；
DNA sequence is as shown in sequence 1；
Characteristic distributions: summer, East China, high bubble phase fermentation liquid；
A2 is Serratia marcescens subsp.Sakuensis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: milky, circular, smooth surface, glossy, neat in edge, easy picking, soluble in water；
Cell characteristic: quarter butt, individually or in pairs exists, does not moves；
DNA sequence is as shown in sequence 2 in sequence table；
Characteristic distributions: summer, North China, cross drainage；
A3 is Aquitalea magnusonii；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: yellow is translucent, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: quarter butt, individually or in pairs exists, motion；
Physiological and biochemical property: Gram-negative, KOH string test is positive, and catalase positive, oxidase positive, to chlorine Change sodium sensitive, belong to Neisseriaceae；
DNA sequence is as shown in sequence 3；
Characteristic distributions: summer, North China, dilution outlet deoxygenated water；
A4 is Chryseobacterium arthrosphaerae；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: yellow, petite, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: quarter butt, individualism, do not move, without spore, 1 μ m 2 μm；
Physiological and biochemical property: Gram-negative, the KOH wire drawing experiment positive, oxidase positive, catalase positive, it is impossible to Growing at 4 DEG C and 45 DEG C, glucose fermentation, maltose, mannose produces acid, lactose fermenters, sucrose, sorbitol etc. on a small quantity Do not produce acid；
DNA sequence is as shown in sequence 4；
Characteristic distributions: summer, southeast, do not sterilize rice wine；
A5 is Enterobacter xiangfangensis；
Strain classification: aerobic bacteria:
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C:
Colony characteristics: yellow (the most orange red), flat sheet-like, Intermediate Gray white, edge is uneven, smooth surface, moistening, Easily provoke, soluble in water；
Cell characteristic: quarter butt, does not moves；
Physiological and biochemical property: Enterobacter xiangfangensis is that a strain is found in Heilongjiang Province, the leather separated in acid bread Lan Shi negative bacterium；
DNA sequence is as shown in sequence 5；
Characteristic distributions: summer, North China, rice wine liquid；
A6 is Macrococcus equipercicus；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: crocus, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: cell spheroid, diameter 1.0～2.0 μm, individually or in pairs exist, accidental tetrad, do not move；
Physiological and biochemical property: Gram-positive is variable to gram；With few flagellar movement；Do not sprout spore；Aerobic；Breathe generation The chemoheterotrophic bacteria thanked；Catalase is positive；The suitableeest 30～37 DEG C；Medium addicted to salt, culture medium need to add 0.5%～20%NaCl；Send out Now in the soil of sea and saliferous；
DNA sequence is as shown in sequence 6；
Characteristic distributions: summer, North China, packing shop surrounding air；
A7 is Ralstonia insidiosa；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: translucent white, circular, petite, neat in edge, smooth surface, easy picking, soluble in water；
Cell characteristic: middle bar, individually or in pairs exists, motion；
DNA sequence is as shown in sequence 7；
Characteristic distributions: summer, North China, deoxygenated water；
A8 is enterobacter asburiae (Enterobacter asburiae)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white, circular, neat in edge, smooth surface, water moistens, glossy, and easy picking is soluble in water；
Cell characteristic: quarter butt, individualism, motion, without spore；
DNA sequence is as shown in sequence 8；
Characteristic distributions: summer, North China, wine filling machine pipeline rice wine；
A9 is Acinetobacter baumannii (Acinetobacter baumannii)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: circular, milky, bacterium colony is bigger, neat in edge, and smooth surface is glossy, and water moistens, thickness, easily Picking, soluble in water；
Cell characteristic: ball-type, individualism, does not moves；
Physiological and biochemical property: Gram-negative, KOH is positive, catalase positive
DNA sequence is as shown in sequence 9；
Characteristic distributions: summer, southeast, filter air bells laid；
A10 is Acinetobacter nosocomialis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: milky, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: coccus, individually or in pairs exists, does not moves；
Physiological and biochemical property: Gram-negative coccobacillus, oxidase negative, power is negative, nitrate negative；
DNA sequence is as shown in sequence 10；
Characteristic distributions: summer, North China, surrounding air；
A11 is chryseobacterium indologenes (Chryseobacterium indologenes)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: yellow, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: middle bar, does not moves, without spore；
Physiological and biochemical property: Gram-negative, direct rod shape, end circle, usually 0.5 μ m 1.0～3.0 μm, does not moves, Fricton-tight or swimming, the most aerobic, external environment separator can be 37 DEG C of growths；At cultured on solid medium, bacterium colony is circular (straight Footpath 1～2nm), protuberance, smooth and glossy, Quan Yuan, catalase, oxidase, phosphatase are the most positive, indigestion agar, Chemoorganotrophy, is produced acid not aerogenesis by carbohydrate in low concentration protein culture medium；
DNA sequence is as shown in sequence 11；
Characteristic distributions: summer, southeast, filter settles；
A12 is Xanthomonas sacchari；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: lemon yellow, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: quarter butt, exists in pairs, size 0.4～1.0 μ m 1.2～3.0 μm, single-ended polar flagella, motion；
Physiological and biochemical property: gram negative bacteria, KOH wire drawing is positive, and oxydase reaction is weak or negative, catalase positive, specially Property aerobic, the highest to nutritional requirement, but growth slower, it is impossible to utilizing the macromolecular substances such as gelatin, starch, growth need to provide paddy Propylhomoserin and methionine, do not carry out nitrate respiration；
DNA sequence is as shown in sequence 12；
Characteristic distributions: summer, North China, deoxidation water pot deoxygenated water；
A13 is He Shi Escherichia (Escherichia hermannii)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white translucent, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: quarter butt, individualism, does not moves, without spore；
DNA sequence is as shown in sequence 13；
Characteristic distributions: summer, North China, surrounding air；
A14 is bacillus amyloliquefaciens solution starch subspecies (Bacillus amyloliquefaciens subsp. Amyloliquefaciens)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white, in the form of sheets, central authorities' bubbling is glossy, and edge is uneven, flat for bacterium colony, matt, thickness, easily Picking, soluble in water；Cell characteristic: quarter butt, in pairs；
DNA sequence is as shown in sequence 14；
Characteristic distributions: summer, East China, bottle washer exports；
A15 is bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white, circular, neat in edge, rough surface, there are year colyliform fold projection in central authorities；
Cell characteristic: middle bar, in chain, produces spore, does not moves；
Physiological and biochemical property: Gram-positive, KOH is negative；
DNA sequence is as shown in sequence 15；
Characteristic distributions: summer, southeast, filter settles；
A16 is giant cell nitrogen Zymomonas mobilis (Azomonas macrocytogenes)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white is the most faint yellow, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: bacterium colony outer layer has film, film part cellular morphology to be spherical to thick bar shape, dense arrangement；Inside there are liquid, liquid Body portion cellular morphology is quarter butt, existence independent paired or in heaps, neither moves, is formed without spore and cyst；
Physiological and biochemical property: Gram’s staining usually feminine gender, the most variable；Aerobic, but can grow under low oxygen pressure；Almost All bacterial strains produce water solublity or fluorochrome；Chemoheterotrophic bacteria；Sugar, alcohol and organic acid and salt growth can be utilized；Fixed nitrogen, Generally symbiotic nitrogen fixation, does not understands casein, but ammonia salt and some aminoacid can be utilized as nitrogen source；Catalase is positive；
DNA sequence is as shown in sequence 16；
Characteristic distributions: summer, North China, wine filling machine standby pressure carbon dioxide；
A17 is radioprotective acinetobacter calcoaceticus (Acinetobacter radioresistens)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: circular, milky, neat in edge, moistening, easy picking, more soluble in water；
Cell characteristic: spherical bacterium, grows in pairs, and do not move (being considered as easily coccus), without spore；
Physiological and biochemical property: Gram-negative, without brood cell and flagellum, unpowered, there is pod membrane；Obligate aerobic, nitrate reaction And oxidase test is negative；
DNA sequence is as shown in sequence 17；
Characteristic distributions: summer, North China, Fructus Hordei Germinatus；
A18 is Bacillus subtilis subsp.inaquosorum；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: milky, circular, bacterium colony is big, and outer layer has film, inside has thick liquid, and edge is uneven or the most uneven, Mattness, easy picking, it is not readily dissolved in water；
Cell characteristic: middle bar, paired or catenation, motion, produce spore；
DNA sequence is as shown in sequence 18；
Characteristic distributions: summer, North China, environment chain road environment fermentation tube sheet environment fermentation tank field；
A19 is bacillus cereus (Bacillus cereus)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white, circular, edge is uneven, and rough surface is matt, and easy picking is soluble in water；
Cell characteristic: middle thick bar, paired or independent or chain, motion, produce spore；
Physiological and biochemical property: Gram-positive, KOH is negative；
DNA sequence is as shown in sequence 19；
Characteristic distributions: summer, southeast, the sedimentation of bright beer tank air, filter air, rice wine, bright beer tank sedimentation；
A20 is Klebsiella quasipneumoniae subsp.quasipneumoniae；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: milky, circular, neat in edge, it is difficult to picking, glossy, surface wettability, thickness talent for swimming, it is difficult to It is dissolved in water；
Cell characteristic: short and thick bar, motion, size 0.5～0.8 μ m 1～2 μm, individually, become double or short catenation；Nothing Brood cell, atrichia, there is thicker pod membrane, majority has pili；
Physiological and biochemical property: gram negative bacilli, catalase positive, relatively can send out on Bacterium entericum selective medium Kefir milk sugar, presents coloured bacterium colony；
DNA sequence is as shown in sequence 20；
Characteristic distributions: summer, East China, pack gland rice wine；
A21 is Ludwig enterobacteria (Enterobacter ludwigii)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: quarter butt, individually or in pairs exists, motion；
Physiological and biochemical property: Gram-negative, the KOH wire drawing experiment positive；
DNA sequence is as shown in sequence 21；
Characteristic distributions: summer, North China, canful fermentation liquid；
A22 is staphylococcus xylosus (Staphylococcus xylosus)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: circular, milk yellow, neat in edge, center projections, smooth surface, moistening, easy picking, soluble in water, Quality is closely knit；
Cell characteristic: spherical, does not moves, single growth, 0.8-1.0 μm；
Physiological and biochemical property: gram positive bacteria, KOH wire drawing experiment feminine gender, can grow at anaerobic environment；Catalase sun Property, oxidase negative, fermentation and acid in glucose, produce acid in xylose, arabinose, fructose, mannose, mannitol, half Lactose, sorbitol, salicin, maltose, lactose, trehalose and glycerol, V-P is positive, 7% sodium chloride broth growth, 45 DEG C Growth, gelatinase is positive, alkaline phosphatase positive, the arginine hydrolysis positive；Staphylocoagulase is negative, and the agglomerate factor is cloudy Property, can not ferment melezitose, sucrose, Raffinose, xylitol, cellobiose, nitrate reduction negative, can not be at 10% atmosphere Changing sodium broth growth, urease negative, DNase is negative, dead drunk dirty digestion feminine gender, and lecithinase is negative, Tween 80 hydrolysis feminine gender；
DNA sequence is as shown in sequence 22；
Characteristic distributions: summer, North China, packing shop bottle washer；
A23 is skin spy's acinetobacter calcoaceticus (Acinetobacter pittii)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: milky, circular, neat in edge, smooth surface, glossy, thickness, easy picking, soluble in water；
Cell characteristic: club shape, in pairs, does not moves；
Physiological and biochemical property: Gram-negative coccobacillus, oxidase negative, power is negative, nitrate negative；
Characteristic distributions: summer, southeast, rice wine before film；
A24 is roost rose of Sharon pseudomonas (Pseudomonas hibiscicola)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white is slightly turned to be yellow, translucent, circular, neat in edge, smooth surface, glossy, easy picking, easily It is dissolved in water；
Cell characteristic: quarter butt, 0.5-1 μ m 1.5-4 μm, individually or in pairs exist, motion；
Physiological and biochemical property: Gram-negative, the KOH wire drawing experiment positive, catalase is positive, oxidase positive；Aerobic, change Can heterotrophism；Gelatin can not liquefy；Nitrate can be reduced into nitrite；C.I. 13020., V-P are negative, edwardsiella hoshinae, no Produce hydrogen sulfide, it is impossible to Starch Hydrolysis；Need not somatomedin；The carbon sources such as glucose, lactate, succinate can be utilized；
DNA sequence is as shown in sequence 23；
Characteristic distributions: summer, southeast, the sedimentation of malt workshop air, bottle washer；
A25 is micrococcus lacticus (Micrococcus lactis)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: lemon yellow, circular, neat in edge, smooth surface, easy picking, soluble in water；
Cell characteristic: coccus, diameter about 1.0 μm, do not move；
Physiological and biochemical property: Gram-positive, does not moves, obligate aerobic, at 10 DEG C to 40 DEG C, then can not give birth to more than 42 DEG C Long, can grow in the range of pH4.5-9.5, optimum pH is 7.5, and the sodium chloride of resistance to 0% to 4% is not given birth at maconkey agar Long, catalase positive, oxidase negative, secrete urase, it is impossible to starch-splitting, Esculin and polysorbas20,40,60 or 80；Lactose, sucrose, cottonseed sugar, Sorbitol, salicin, carubinose, trehalose, d-galactose, n-can not be utilized Acetyl-d-glucosamine, methyl β-d-glucopyranoside or amylopectin；
DNA sequence is as shown in sequence 24；
Characteristic distributions: summer, East China, filling machine surrounding air；
A26 is husky good fortune bacillus cereus (Bacillus safensis)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: milky, irregularly, neat in edge, concavity is matt, and marginal swell is glossy, easy picking, More soluble in water；
Cell characteristic: quarter butt, 0.5-0.7 μ m 1.0-1.2 μm individually or in pairs exists, motion；
Physiological and biochemical property: Gram-positive, KOH wire drawing is negative, catalase positive, oxidase positive, resistance to pH5.6 The sodium chloride of 0%-10%, growth temperature 10-50 DEG C, optimum growth temperature 30-37 DEG C, it is impossible to be grown on 4 DEG C or 50 DEG C；
DNA sequence is as shown in sequence 25；
Characteristic distributions: summer, North China, the residual wine of secondary, deoxidation water pot deoxygenated water, environment rice wine tube sheet；
A27 is basophilic Exiguobacterium sp (Exiguobacterium alkaliphilum)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: milky, neat in edge, smooth surface, thickness, easy picking, soluble in water；
Cell characteristic: gram positive bacteria, amphimicrobian, children's culture in age is brevibacterium, and old culture is spherical, 1.1～1.2 μ m 1.4～3.2 μm, does not give birth to spore, the most antiacid, moves with peritrichous；
Physiological and biochemical property: basophilic, can be in pH6.5～11.5 growth；Chemoheterotrophic bacteria, fermentating metabolism；From glucose, sugarcane Sugar, galactose and some other sugar produce acid；Basophilic, primary product is lactic acid, acetic acid and formic acid；Catalase is positive, oxidase Negative；Reduction nitrate；Gelatin hydrolysate, starch and casein；Optimum growth temperature 37 DEG C；
DNA sequence is as shown in sequence 26；
Characteristic distributions: summer, East China, filling machine surrounding air；
A28 is aerosporus (Bacillus aerophilus)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: milk yellow, circular, central authorities' fold, bacterium colony is little, is dried, easy picking, it is more difficult to be dissolved in water；
Cell characteristic: middle bar, motion, amphitrichous；
Physiological and biochemical property: gram positive bacteria, KOH is negative, and catalase is positive, and optimum growth temperature is 30 DEG C；
DNA sequence is as shown in sequence 27；
Characteristic distributions: summer, North China, yeasting air；
A29 is Burkholderia arboris；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: yellow green, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: middle bar, individualism, motion, without spore；
Physiological and biochemical property: Gram-negative, the KOH wire drawing experiment positive, oxidase positive, catalase positive, nitric acid Salt is the most Antigen positive hybridomas；Can grow at a temperature of 30 DEG C with 37 DEG C, minority can grow at 42 DEG C；Glucose fermentation, sucrose, wheat Bud sugar, lactose and ribitol produce acid；
DNA sequence is as shown in sequence 28；
Characteristic distributions: summer, southeast, rice wine after film；
A30 is anthrax bacillus (Bacillus anthracis)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics (A in Figure 30): white, circular, edge is the most uneven, central authorities' microprotrusion, and mattness is easily chosen Take, soluble in water；
Cell characteristic (B in Figure 30): thick bar, does not moves, and produces spore, catenation；
DNA sequence is as shown in sequence 29；
Characteristic distributions: summer, winter, North China, coarse filtration exports last flushing water, bright beer tank rice wine, bottle washer, does not kills Bacterium free and easy oxygen water pot deoxygenated water, alcoholic air；
A31 is Bacillus tequilensis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white, in the form of sheets, flat, central authorities' white microprotrusion and tarnish, edge is uneven and relatively gloss, and in Part between the heart and edge is glossy, smooth surface, easy picking, soluble in water, produces spore regional centralized, without the spore expanded Capsule；
Cell characteristic: middle bar, 0.9 μ m 4.0 μm, independent or paired, produce spore, do not move；
Physiological and biochemical property: Gram-positive, KOH wire drawing is negative, catalase positive, oxidase positive, can be in Growing in the range of pH5.5-8.0,25-50 DEG C, fermentation glycerol, xylose, glucose etc. produce acid；
DNA sequence is as shown in sequence 30；
Characteristic distributions: summer, North China, empty bottle, ferment training wind；
A32 is soil bacillus brevis (Brevibacillus agri)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: circular, milky, petite, neat in edge, glossy, moistening, soluble in water, easy picking；
Cell characteristic: quarter butt, motion, grow in pairs, raw in spore, do not expand, spore is oval；
Physiological and biochemical property: kOH is negative, Gram-positive, and V-P is negative；Do not hydrolyze starch but liquefaction gelatin；Can be in nutrient meat Soup grows；Glucose produces acid not aerogenesis；
DNA sequence is as shown in sequence 31；
Characteristic distributions: summer, North China, two assure road rice wine；
A33 is Exiguobacterium indicum；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: translucent, faint yellow, flat, irregularly, edge is uneven, smooth surface, and easy picking is soluble in water；
Cell characteristic: shaft-like, in chain or nodular, does not moves, and children's culture in age is bacillus, and old culture is spherical；
Physiological and biochemical property: Gram-positive, KOH wire drawing experiment feminine gender, catalase positive, oxidase positive, growth At 5-30 DEG C and pH value 6-10；It is 7.0 with optimum pH that optimum temperature is 25 DEG C；The normal saline of resistance to 5.8%, it is possible to Salt-free ambient growth；
DNA sequence is as shown in sequence 32；
Characteristic distributions: summer, winter, southeast, filter outlet rice wine, bright beer tank ambient air, bottle washer sink Fall, bottle-washing water；
A34 is Deinococcus ficus；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: circular, blush, smooth surface, neat in edge, easy picking, not thickness, soluble in water；
Cell characteristic: middle bar, independent or paired, do not move, without spore；
Physiological and biochemical property: gram positive bacteria, KOH tests feminine gender, oxidase positive；UV resistant irradiation (254nm, 8-10 Centimetres 10 minutes), available following compound is as sole carbon source: dextrin, polysorbate40, Tween 80, fructose, maltose, Maltotriose, Sorbitol, stachyose, sucrose, acetic acid, lactic acid, methyl pyruvate, monomethyl succinate, propanoic acid, acetone Acid, succinic acid, alanine etc.；
DNA sequence is as shown in sequence 33；
Characteristic distributions: summer, North China, pack environment air；
A35 is Bacillus siamensis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: milky, edge is uneven and in divergent shape, circular, and middle white becomes clear than edge, and easy picking is not done Dry, soluble in water；
Cell characteristic: middle stock, motion, independent, paired or bunchiness growth, peritrichous, 0.3-0.6 μ m 1.5-3.5 μm, elliptic spore is in the middle of thalline or end expands；
Physiological and biochemical property: Gram-positive, KOH is negative, amphimicrobian, and peritrichous can be grown on MacConkey fine jade Fat but cholate medium can not be grown on, catalase positive, oxidase negative, caseinhydrolysate, Esculin, gelatin, DNA And starch；
DNA sequence is as shown in sequence 34；
Characteristic distributions: summer, East China, packing shop compression CO2；
A36 is Xin Shi acinetobacter calcoaceticus (Acinetobacter schindleri)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white translucent, circular, edge is the most uneven, smooth surface, easy picking, soluble in water；
Cell characteristic: spherical, individually or in pairs exists, does not moves；
Physiological and biochemical property: Gram-negative coccobacillus, oxidase negative, power is negative, nitrate negative Grain-negative ball Bacillus, oxidase negative, power is negative, and nitrate negative, glucose fermentation does not produce acid；
DNA sequence is as shown in sequence 35；
Characteristic distributions: summer, North China, ferment tube sheet；
A37 is Young citric acid bacillus (Citrobacter youngae)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: circular, milky, thalline is less, neat in edge, and water moistens, glossy, soluble in water, easy picking；
Cell characteristic: middle bar, single and paired, diameter about 1.0, long 2.0～6.0 μm, motion, there is peritrichous；
Physiological and biochemical property: gram negative bacteria, KOH tests the positive, oxidase negative, and catalase is positive, can utilize Fructus Citri Limoniae Hydrochlorate is as only carbon source；Do not produce phenylalanine deaminase, gelatinase, lipase and DNA enzymatic；Glucose fermentation produces acid and produces Gas, methyl red test is positive, and VP tests the positive；
DNA sequence is as shown in sequence 36；
Characteristic distributions: summer, North China, bright beer tank surrounding air；
A38 is Deinococcus radioduran；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: orange red, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: cell spheroid, diameter 0.5～3.5 μm, paired or tetrad, is big than other coccuses；Do not move, no Produce spore；
Physiological and biochemical property: Gram-positive, but cell wall composition is more complicated, has which floor structure, the change energy of tool respiratory metabolism Heterotrophic bacteria, become inactive tendency to saccharide；Catalase is positive；High resistance gamma-radiation, containing substantial amounts of palmitate；Thermophilic Spend 30～37 DEG C；
Characteristic distributions: summer, North China, environment fine straining；
A39 is Bacillus infantis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: pink, irregularly, flat, smooth surface, easy picking, soluble in water；
Cell characteristic: middle quarter butt, individualism, does not moves, and produces spore；
Physiological and biochemical property: Gram-positive, KOH string test is negative, catalase positive, and oxidase negative is permissible Utilize galactose, glucose, fructose, mannitol, Sorbitol, aesculin, salicin, maltose, 6-(.alpha.-D-galactosido)-D-glucose., sucrose, Trehalose, Raffinose, starch, glycogen, gluconic acid, cellobiose, lactose and inulin etc., be unfavorable for utilizing glycerol, ribose, Mannose, inositol, xylitol, gentiobiose etc.；
DNA sequence is as shown in sequence 37；
Characteristic distributions: summer, North China, tank fine straining；
A40 is Vietnam's bulkholderia cepasea (Burkholderia vietnamiensis)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: milky, circular, less during colony growth 1d, there is microscopic folds on surface, but glossy, colony growth During more than 2d, bacterium colony is full, smooth surface, and fold disappears, glossy；
Cell characteristic: quarter butt, individually or in pairs exists, and motion, without spore；
Physiological and biochemical property: Gram-negative, the KOH wire drawing experiment positive, chemoheterotrophy, reducible nitrate becomes nitrite； Oxidase positive, arginine dihydrolase is negative；Liquefiable gelatin, edwardsiella hoshinae, do not produce H2S；Acid is produced from glucose； Citrate, adipate, D-alanine, Valine, leucine and ortho-aminobenzoic acid can be utilized, it is impossible to utilize Mus Lee's sugar, maltose, tartrate and erythritol；
DNA sequence is as shown in sequence 38；
Characteristic distributions: summer, North China, residual wine；
A41 is Bacillus aryabhattai；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white, circular, there are a discoid slight convex in central authorities, neat in edge, and smooth surface is glossy, easily chooses Take, soluble in water；
Cell characteristic: long thick bar, paired or catenation, do not move, without spore；
DNA sequence is as shown in sequence 39；
Characteristic distributions: winter, North China, alcoholic workshop air, packaging air；
A42 is Enterobacter mori；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: quarter butt, individually or in pairs exists, motion；
DNA sequence is as shown in sequence 40；
Characteristic distributions: winter, southeast, Fructus Hordei Germinatus before pulverizing；
A43 is Cronobacter dublinensis subsp.Lausannensis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: milky, circular, neat in edge, smooth surface, bacterium colony is raised slightly, and center of inside has inconspicuous circle Ring, corrugationless, easy picking, soluble in water；
Cell characteristic: quarter butt, motion, without spore；
DNA sequence is as shown in sequence 41；
Characteristic distributions: winter, North China, southeast, the residual wine of bright beer tank ambient air, secondary, pulverize before Fructus Hordei Germinatus, Thick (smart) rice wine of film；
A44 is Pantoea calida；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: milky, circular, neat in edge, colony edge is transparent, smooth surface, and water moistens, glossy, easily chooses Take, soluble in water；
Cell characteristic: quarter butt, individually or in pairs exists, and motion, without spore；
DNA sequence is as shown in sequence 42；
Characteristic distributions: winter, southeast, sterilization machine ambient air；
A45 is excrement shortwave Zymomonas mobilis (Brevundimonas faecalis)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: pink colour, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: ball, to quarter butt, individually, in pairs or catenation, does not moves, without spore；
DNA sequence is as shown in sequence 43；
Characteristic distributions: winter, North China, between fermented sake ullage；
A46 is staphylococcus saprophyticus (Staphylococcus saprophyticus subsp.Saprophyticus)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: milky, circular, petite, neat in edge, smooth surface, glossy, easy picking, it is not readily dissolved in Water；
Cell characteristic: coccus, in thyrsiform, does not moves；
DNA sequence is as shown in sequence 44；
Characteristic distributions: winter, North China, sterile wind；
A47 is China Al Kut Salmonella in the last of the ten Heavenly stems (Kurthia huakuii)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white, lamellar, flat, edge is uneven, and smooth surface is glossy, and easy picking is soluble in water；
Cell characteristic: white, lamellar, flat, edge is uneven, and smooth surface is glossy, and easy picking is soluble in water；
DNA sequence is as shown in sequence 45；
Characteristic distributions: winter, southeast, Fructus Hordei Germinatus before pulverizing；
A48 is Kurthia gibsonii (Kurthia gibsonii)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: lemon yellow, irregularly, flat, edge is uneven, and surface is more smooth, glossy, easy picking, is soluble in Water；
Cell characteristic: quarter butt, individualism, moves (rotation), without spore；
DNA sequence is as shown in sequence 46；
Characteristic distributions: winter, North China, yeasting air；
A49 is food methanol bacillus cereus (Bacillus methylotrophicus)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white, lamellar, irregularly, edge is uneven, matte surface, matt, and easy picking is not readily dissolved in water；
Cell characteristic: middle stock, long chain, does not moves, has spore；
DNA sequence is as shown in sequence 47；
Characteristic distributions: winter, East China, brewing water；
A50 is Pseudomonas graminis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: milky, circular, petite, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: middle thick bar, individually or in pairs exists, motion, has spore；
DNA sequence is as shown in sequence 48；
Characteristic distributions: winter, North China, yeasting air；
A51 is simple bacillus cereus (Bacillus simplex)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white, circular, flat, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: stock, paired or catenation, do not move, without spore；
DNA sequence is as shown in sequence 49；
Characteristic distributions: winter, North China, alcoholic workshop air；
A52 is Lysinibacillus xylanilyticus；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white, flat, irregularly, edge is uneven, and smooth surface is glossy, and easy picking is soluble in water；
Cell characteristic: middle stock, does not moves, and single-ended spore also expands, individualism, rare paired existence；
DNA sequence is as shown in sequence 50；
Characteristic distributions: winter, southeast, wine filling machine ambient air；
A53 is Chryseobacterium hispalense；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: crocus, circular, neat in edge, smooth surface, water moistens, glossy, and easy picking is soluble in water；
Cell characteristic: quarter butt, does not moves, individualism；
DNA sequence is as shown in sequence 51；
Characteristic distributions: winter, East China, rice wine pipeline；
A54 is Kocuria marina；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: lemon yellow, circular, bacterium colony is little, neat in edge, and smooth surface is glossy, and easy picking is soluble in water；
Cell characteristic: coccus, individually or in pairs exists, does not moves, without spore；
Characteristic distributions: winter, southeast, bottle washer, wine filling machine ambient air；
A55 is Pseudomonas kunmingensis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: milk yellow, irregularly, edge is uneven dissipates growth, rough surface to surrounding, and glossy, easy picking is easy It is dissolved in water；
Cell characteristic: quarter butt, individually or in pairs exists, and motion, without spore；
DNA sequence is as shown in sequence 52；
Characteristic distributions: winter, North China, wine filling machine standby pressure carbon dioxide；
A56 is Paenibacillus humicus；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white, circular, neat in edge, smooth surface, water moistens, glossy, in muddy sample inside bacterium colony, easily Picking, soluble in water；
Cell characteristic: quarter butt, motion, individually, there is spore；
DNA sequence is as shown in sequence 53；
Characteristic distributions: winter, East China, finished wine；
A57 is U.S. bar bacterium (Corynebacterium callunae)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: lemon yellow, petite, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: ball to quarter butt or bend shaft-like, individually, in pairs or bunchiness arrangement, does not moves, without spore；
DNA sequence is as shown in sequence 54；
Characteristic distributions: winter, North China, alcoholic workshop air；
A58 is Cedecea neteri (Cedecea neteri)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white, irregularly, edge is uneven, and smooth surface is glossy, and easy picking is soluble in water；
Cell characteristic: be as short as middle bar, individually or in pairs arrange, moves (moving about), without spore；
DNA sequence is as shown in sequence 55；
Characteristic distributions: winter, North China, sterilization time wine；
A59 is brevibacterium frigoritolerans (Brevibacterium frigoritolerans)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: milky, circular, neat in edge, there is circular projection on surface, glossy, and easy picking is soluble in water；
Cell characteristic: middle stock, chain is rehearsed, is not moved, without spore；
DNA sequence is as shown in sequence 56；
Characteristic distributions: winter, North China, alcoholic workshop air；
A60 is lemon yellow bacillus pumilis (Curtobacterium citreum)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: milky white the most faint yellow, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: quarter butt, individually or in pairs exists, and motion, without spore；
DNA sequence is as shown in sequence 57；
Characteristic distributions: winter, southeast, bright beer tank ambient air；
A61 is Bacillus panaciterrae；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white, in the form of sheets, irregularly, edge is uneven, rough surface for bacterium colony, and center projections has fold, easily Picking, soluble in water；
Cell characteristic: stock, catenation, do not move, produce spore；
DNA sequence is as shown in sequence 58；
Characteristic distributions: winter, East China, bottle washer ambient air；
A62:Chryseobacterium hominis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white, circular, petite, neat in edge, smooth surface, glossy, easy picking, it is not readily dissolved in water；
Cell characteristic: middle bar, individually or in pairs exists, does not moves, without spore；
DNA sequence is as shown in sequence 59；
Characteristic distributions: winter, North China, pack air；
A63: sphingomonas paucimobilis (Sphingomonas paucimobilis)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: lemon yellow, circular, bacterium colony is little, neat in edge, and smooth surface is glossy, and easy picking is soluble in water；
Cell characteristic: quarter butt, individually or in pairs exists, motion；
DNA sequence is as shown in sequence 60；
Characteristic distributions: winter, East China, finished wine；
A64 is Erwinia tasmaniensis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: yellow is translucent, early growth period is faint yellow, flat, and there are a year colyliform depression, neat in edge, table in central authorities Face is smooth, glossy, easy picking, soluble in water；
Cell characteristic: quarter butt, individually or in pairs exists, does not moves；
DNA sequence is as shown in sequence 61；
Characteristic distributions: winter, southeast, Fructus Hordei Germinatus before pulverizing；
A65 is Pseudomonas aeruginosa (Pseudomonas aeruginosa)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: milk yellow, translucent, lamellar, irregularly, edge is uneven, and smooth surface is glossy, at reflection light Irradiating lower periphery of bacterial colonies is blueness, and chromogenic element, culture medium darkens, easy picking, soluble in water；
Cell characteristic: quarter butt, individually or in pairs exists, and motion, without spore；
DNA sequence is as shown in sequence 62；
Characteristic distributions: winter, southeast, brewing water；
A66 is pseudo-blood Sphingol single-cell (Sphingomonas pseudosanguinis)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: yellow, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: be as short as middle bar, individualism, motion
DNA sequence is as shown in sequence 63；
Characteristic distributions: winter, southeast, the residual wine of secondary；
A67 is nicotinic acid bacillus cereus (Bacillus niacini)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: white, irregularly, neat in edge, smooth surface, matt, easy picking, soluble in water；
Cell characteristic: middle stock, individually or in pairs exists, does not moves；
DNA sequence is as shown in sequence 64；
Characteristic distributions: winter, North China, alcoholic workshop air；
A68 is acetyl Exiguobacterium sp (Exiguobacterium acetylicum)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: translucent, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: short and thick to long thick bar or bending, paired or catenation, do not move, without spore；
DNA sequence is as shown in sequence 65；
Characteristic distributions: winter, North China, pack air；
A69 is Deinococcus caeni；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: crocus, circular, more flat, neat in edge, there is slight fold on surface, more glossy, easy picking, Soluble in water；
Cell characteristic: middle thick bar, independent, paired or catenation, do not move；
DNA sequence is as shown in sequence 66；
Characteristic distributions: winter, North China, packing shop surrounding air；
A70 is Deinococcus grandis；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: crocus, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: short and thick bar, individually or in pairs exists, does not moves；
DNA sequence is as shown in sequence 67；
Characteristic distributions: winter, southeast, filter outlet rice wine；
A71 is plant bacillus pumilis (Curtobacterium plantarum)；
Strain classification: aerobic bacteria；
Condition of culture: plate count agar culture medium, cultivates 24 hours for 37 DEG C；
Colony characteristics: faint yellow, translucent, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: quarter butt, individually or in pairs exists, and motion, without spore；
DNA sequence is as shown in sequence 68；
Characteristic distributions: winter, East China, Fructus Hordei Germinatus, wine filling machine ambient air before finished product rice wine, pulverizing；
B1 is Leuconostoc mesenteroides dextran subspecies (Leuconostoc mesenteroides subsp.dextranicun)；
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics: circular, milky, smooth surface, neat in edge, easy picking, soluble in water；
Cell characteristic: quarter butt, exists in pairs, and cell size is 0.5～0.7 × 0.7～1.2mm, does not moves, without spore；
Physiological and biochemical property: gram positive bacteria, KOH is negative, and catalase is negative, glucose fermentation, fructose, lactose, half Lactose, sucrose, maltose, mannose, trehalose, do not hydrolyze arginine；Growth temperature range 2 DEG C-53 DEG C, the most suitable growth Temperature 3O DEG C-40 DEG C；Acid resistance is strong, and growth optimum pH is 5.5～6.2, can grow in the environment of pH≤5, and in Property or initial alkalescence under the conditions of growth rate reduce；
DNA sequence is as shown in sequence 69；
Characteristic distributions: summer, North China, pack bottle washer surrounding air；
B2 is Lactobacillus brevis (lactobacillus brevis)；
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics: circular, milky, edge is uneven, rough surface, moistening, and easy picking is soluble in water；
Cell characteristic: middle stock, does not moves, single growth；
Physiological and biochemical property: Gram-positive, KOH is negative, and negative catalase can utilize fructose, arabinose, honey Disaccharide, xylose produce acid；
DNA sequence is as shown in sequence 70；
Characteristic distributions: summer, winter, North China, air, dilution after rice wine, pipeline rice wine, coarse filtration machine outlet rice wine, Yeasting air, C0 enter bright beer tank rice wine, bright beer tank rice wine for after yeast height bubble phase, chain of packages road surrounding air, dilution；
B3 is Lactococcus garvieae (Lactococcus garvieae)；
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics: milky, circular, smooth surface, easy picking, soluble in water, thalline is less；
Cell characteristic: quarter butt, do not move；
Physiological and biochemical property: without spore, G+ coccus, bacterium colony smooth surface is glossy, low projection, edge is complete, and thickness is yellowish The opaque circular colonies of color；Thalline is ellipsoid, in pairs, becomes chain alignment；Acid not aerogenesis can be produced, it is impossible to utilize starch from glucose, Milk acidification can be made；Resistance to 4%NaCl；
DNA sequence is as shown in sequence 71；
Characteristic distributions: filling machine in winter in summer standby pressure CO2；
B4 is Lactobacillus paracasei subsp.tolerans；
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics: circular, white, smooth surface, neat in edge, easy picking, soluble in water；
Cell characteristic: middle stock, bending, individually grow, do not move；
Physiological and biochemical property: can be 10 DEG C and 37 DEG C growths, 40 DEG C then can not grow, 72 DEG C, 40s still can survive, fermentation produce Lactic acid, glucose fermentation, fructose, lactose, mannose, n acetylglucosamine n etc. produce acid；Urease negative, does not hydrolyze Esculin；
DNA sequence is as shown in sequence 72；
Characteristic distributions: summer, North China, pipeline rice wine；
B5 is cibarium Wei Si Salmonella (Weissella cibaria)；
Strain classification: anaerobic bacteria；
Condition of culture: MRS agar culture medium, cultivates 5d for 28 DEG C；
Colony characteristics: white, circular, bacterium colony is little, smooth surface, neat in edge；
Cell characteristic: quarter butt, does not moves, and individually or in pairs exists；
Physiological and biochemical property: gram positive bacteria, is formed without spore, does not moves, and does not utilize rhamnose, can utilize most of carbon Source, utilizes arginine, and decomposition glucose produces CO2, acidproof, optimum pH 5.5-6.2；
DNA sequence is as shown in sequence 73；
Characteristic distributions: summer, southeast, bright beer tank air；
B6 is Pediococcus claussenii；
Strain classification: amphimicrobian；
Condition of culture: MRS agar culture medium, cultivates 5d for 28 DEG C；
Colony characteristics: milky, circle, neat in edge, picking soluble in water, easy；
Cell characteristic: spherical, do not move, diameter 1.2～2.0 μm；
DNA sequence is as shown in sequence 74；
Characteristic distributions: summer, East China, canful rice wine；
B7 is Pediococcus lolii；
Strain classification: anaerobic bacteria
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C
Colony characteristics: milky, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water
Cell characteristic: coccus, independent, paired or tetrad arrangement, do not move
DNA sequence is as shown in sequence 75；
Characteristic distributions: summer, southeast, wine filling machine water；
B8 is leuconostoc lactis (Leuconostoc holzapfelii)；
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics: milky, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: coccus, in catenation, does not moves；
Physiological and biochemical property: Gram-positive, KOH wire drawing experiment feminine gender, catalase is negative, glucose fermentation, fructose, breast Sugar, sucrose, maltose, galactose；Azymic cellobiose, xylose；Do not hydrolyze arginine, amphimicrobian；
DNA sequence is as shown in sequence 76；
Characteristic distributions: summer, North China, Fructus Hordei Germinatus before pulverizing；
B9 is Lactococcus lactis subsp.tructae；
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics: white, translucent, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: coccus, 0.5-1.2 μ m 0.5-1.5 μm is catenation, does not moves；
Physiological and biochemical property: Gram-positive, amphimicrobian, chemoheterotrophy, fermentating metabolism, with some carbohydrate fermentation Produce acid, based on L (+)-lactic acid, not aerogenesis；Nutritional requirement is complicated, and catalase is negative, oxidase negative；Optimum temperature 30 DEG C, The sodium chloride of resistance to 4%, fermentating maltose, lactose, ribose, sucrose, mannitol etc. produce acid；
DNA sequence is as shown in sequence 77；
Characteristic distributions: summer, North China, environment coarse filtration；
B10 is lactococcus lactis subsp.cremoris (Lactococcus lactis subsp.cremoris)；
Strain classification: amphimicrobian；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics: circular, petite, white, smooth surface, neat in edge, easy picking, not thickness, soluble in water；
Cell characteristic: coccus, paired or bunchiness, 0.5～1.2 μ m 0.5～1.5 μm, do not move, spore of not sprouting, without pod Film；
Physiological and biochemical property: gram positive bacteria, KOH tests feminine gender, and catalase is negative, oxidase negative, chemoheterotrophy, Fermentating metabolism, with some carbohydrate fermentation produce acid, based on L (+)-lactic acid, not aerogenesis；Nutritional requirement is complicated,；The suitableeest Temperature 30 DEG C, can be 10 DEG C of growths, it is impossible to grow under the conditions of 45 DEG C, be weak to the NaCl of 4%, can utilize glucose, gala Sugar, maltose and mannitol, it is impossible to utilize inulin, produce extracellular polysaccharide；
Characteristic distributions: summer, North China, Wort oxygenating sterile wind；
B11 is Pediococcus pentosaceus (Pediococcus pentosaceus)；
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics: circular, white, neat in edge, smooth surface, moistening, easy picking, soluble in water；
Cell characteristic: coccus, tetrad or in thyrsiform；
Physiological and biochemical property: Gram-positive, KOH feminine gender optimum growth temperature 35 DEG C, maximum growth temperature 42-45 DEG C；
DNA sequence is as shown in sequence 78；
Characteristic distributions: summer, North China, Fructus Hordei Germinatus before pulverizing；
B12 is Lactobacillus plantarum plant subspecies (Lactobacillus plantarum subsp.plantarum)
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics: circular, milky, bacterium colony is full, smooth surface, neat in edge, easy picking, soluble in water；
Cell characteristic: straight or curved is shaft-like, single, sometimes paired or one-tenth chain, does not moves；
Physiological and biochemical property: gram positive bacteria, KOH tests feminine gender, negative catalase, and optimum growth temperature is 30～35, Optimum pH about 6.5, belongs to homofermentative lactic bacteria；
DNA sequence is as shown in sequence 79；
Characteristic distributions: summer, North China, yeasting air, coarse filtration machine export last flushing water, C0 for yeast height bubble the phase, Fermentation deoxygenated water；
B13 is leuconostoc pseudomesenteroides (Leuconostoc pseudomesenteroides)；
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics: white, micro-Huang, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: quarter butt, individually or in pairs exists, does not moves, without spore；
DNA sequence is as shown in sequence 80；
Characteristic distributions: winter, North China, fine straining outlet wine liquid；
B14 is Clostridium beijerinckii (Clostridium beijerinckii)；
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics: white, irregularly, edge is uneven, and smooth surface is glossy, and easy picking is soluble in water；
Cell characteristic: stock, the long thick bar of minority, individually or in pairs exist, move (moving about), without spore；
DNA sequence is as shown in sequence 81；
Characteristic distributions: winter, North China, fine straining outlet wine liquid；
B15 is for solving ornithine Raoul bacterium (Raoultella ornithinolytica)；
Strain classification: anaerobic bacteria；
Condition of culture: NBBA culture medium, cultivates 5-7 days for 28 DEG C；
Colony characteristics: white, translucent, circular, neat in edge, smooth surface, glossy, easy picking, soluble in water；
Cell characteristic: middle thick bar, individualism, does not moves, without spore；
DNA sequence is as shown in sequence 82；
Characteristic distributions: winter, North China, pack rice wine pipeline rice wine；
C1 is Aureobasidium；
Strain classification: fungus；
Condition of culture: potato dextrose agar, cultivates 7 days for 27 DEG C；
Colony characteristics: white, irregular or circular, neat in edge, smooth surface, easy picking, soluble in water；
Cell characteristic: cell ovalize two ends are the sharpest；
DNA sequence is as shown in sequence 83；
Characteristic distributions: winter, southeast, fermentation plant surrounding air；
C2 is Rhodotorula mucilaginosa；
Strain classification: fungus；
Condition of culture: potato dextrose agar, cultivates 7 days for 27 DEG C；
Colony characteristics: 7d, 13mm, milky, circular, neat in edge, water moistens, and smooth surface is glossy, quality paste, Easily picking, is not readily dissolved in water (yeast)；
Cell characteristic: circular thalline, single-ended budding；
DNA sequence is as shown in sequence 84；
Characteristic distributions: winter, Shenyang, environment rice wine tube sheet；
C3 is Cryptococcus rajasthanensis；
Strain classification: fungus；
Condition of culture: potato dextrose agar, cultivates 7 days for 27 DEG C；
Colony characteristics: 7d, 10mm, red, irregularly, edge is uneven, and there is fold on surface, matt；
Cell characteristic: yeast, thalline is oval to circular, without false silk
DNA sequence is as shown in sequence 85；
Characteristic distributions: winter, North China, bottle cap.
9. according to the arbitrary described system of claim 4-8, it is characterised in that: described data base has also stated that the micro-life of described pollution The probability of happening of thing；
In described data base, the probability of happening of described described contaminating microorganisms is specific as follows:
Summer In North China aerobic bacteria is at each production process probability of happening:
Top box or galenic bottle cap: bacillus subtilis 11%, pantoea agglomerans 55%, Soil Bacillus 13%；
Dilution deoxidation water pot: acinetobacter calcoaceticus 25%；
Dilution oxygen remove exports deoxygenated water: Bacillus licheniformis 40%, Bacillus pumilus 17.5%, plants raw Raoul bacterium 17.3%；
The strainer last flushing water of outlet: bacillus amyloliquefaciens 50%, Bacillus coagulans 45%；
Coarse filtration machine outlet rice wine liquid: bacillus subtilis 38%；
Storage wine phase fermentation liquid: bacillus amyloliquefaciens 20%, Acetobacter pasteurianus 15%；
High bubble phase fermentation liquid: bacillus amyloliquefaciens 36%；
Canful fermentation liquid: bacillus amyloliquefaciens 35%, bacillus subtilis 19%；
Softening water (brewing water): Bacillus coagulans 30%, streptococcus thermophilus 7%；
Summer In North China anaerobe is at each production process probability of happening:
Rice wine after gland: zymomonas mobilis 45%；
Rice wine pipeline: Lin Denaishi lactobacillus 23%, Lactobacillus brevis 20%, Lactobacillus buchneri 10%；
The residual wine of secondary: zymomonas mobilis 28%, Lactobacillus brevis 23%；
Bright beer tank rice wine liquid is entered: Lin Denaishi lactobacillus 46%, pantoea agglomerans 45%, Lactobacillus buchneri 15% after dilution；
Storage wine phase fermentation liquid: zymomonas mobilis 6%, Lin Denaishi lactobacillus 10%；
Canful fermentation liquid: zymomonas mobilis 8%, Pectinatus 23%；
Softening water: leukonid 10%；
North China aerobic bacteria in winter is at each production process probability of happening:
Rice wine after film: plant raw Raoul bacterium 66%；
Sharp foam water: Bacillus coagulans 9%；
The residual wine of secondary: Bacillus coagulans 83%；
Dilution oxygen remove exports deoxygenated water: Bacillus coagulans 7%；
The strainer last flushing water of outlet: Bacillus coagulans 33%；
The coarse filtration machine last flushing water of outlet: acinetobacter calcoaceticus 20%；
The filter last flushing water of outlet: Bacillus coagulans 33%；
CIP hot-water cylinder hot water: Bacillus coagulans 1%, bacillus amyloliquefaciens 11%, Bacillus pumilus 1%；
Softening water (brewing water): acetobacter aceti 6%；
North China anaerobe in winter is at each production process probability of happening:
Rice wine after film: Lin Denaishi lactobacillus 26%；
Wine filling machine standby pressure carbon dioxide, compressed air: Pectinatus 13%；
The residual wine of secondary: Lin Denaishi lactobacillus 55%；
Canful fermentation liquid: Lactobacillus brevis 14%；
Co is for yeast spread cultivation liquid: Pectinatus 20%；
East China aerobic bacteria in summer is at each production process probability of happening:
Rice wine after gland: pantoea agglomerans 20%；
Rice wine pipeline: pantoea agglomerans 54%, bacillus amyloliquefaciens 32%, Acetobacter pasteurianus 24%；
The residual wine of secondary: streptococcus thermophilus 24%；
Bright beer tank rice wine liquid is entered: plant raw Raoul bacterium 36% after dilution；
Canful fermentation liquid: pantoea agglomerans 10%, Acetobacter pasteurianus 6%, Bacillus coagulans 6%；
Soften water: plant raw Raoul bacterium 32%；
East China anaerobe in summer is at each production process probability of happening:
Wine filling machine standby pressure carbon dioxide, compressed air: zymomonas mobilis 36.25%；
The residual wine of secondary: Pediococcus pentosaceus 12%, mound shape lactobacillus 10%；
Bright beer tank rice wine liquid is entered: zymomonas mobilis 57%, Lactobacillus brevis 26% after dilution；
Filter outlet rice wine liquid: Lactobacillus brevis 4%, pantoea agglomerans 17%；
Storage wine phase fermentation liquid: zymomonas mobilis 44%, Lactobacillus brevis 19%, Lactobacillus buchneri 5%；
High bubble phase fermentation liquid: zymomonas mobilis 5%, harmful sheet coccus 25%, lactobacillus casei 9%；
Canful fermentation liquid: Lactobacillus brevis 30%；
East China aerobic bacteria in winter is at each production process probability of happening:
High bubble phase fermentation liquid: plant raw Raoul bacterium 76%, Bacillus pumilus 2%；
Canful fermentation liquid: Acetobacter pasteurianus 88%, Bacillus circulans 6%；
CIP hot-water cylinder hot water: Bacillus circulans 34%；
Aseptic water pot: bacillus subtilis 25%；
East China anaerobe in winter is at each production process probability of happening:
Rice wine pipeline: Lactobacillus brevis 5%, lactobacillus casei 18%；
The residual wine of secondary: Lin Denaishi lactobacillus 23%, Lactobacillus buchneri 35%；
Canful fermentation liquid: lactobacillus casei 59%, Lin Denaishi lactobacillus 19%；
Aseptic water pot: Lactobacillus brevis 83%；
Southeast aerobic bacteria in summer is at each production process probability of happening:
Rice wine before film: acinetobacter calcoaceticus 36%；
Top box or galenic bottle cap: Bacillus pumilus 13%, Acetobacter pasteurianus 13%, pseudomonas 6%；
Sharp foam water: Bacillus licheniformis 26%；
The residual wine of secondary: Bacillus licheniformis 7%, streptococcus thermophilus 36%, Bacillus pumilus 16%；
Bright beer tank rice wine liquid is entered: Bacillus licheniformis 20%, bacillus subtilis 23%, chryseobacterium indologenes 6% after dilution；
Softening water: Bacillus coagulans 70%, acinetobacter calcoaceticus 13%, chryseobacterium indologenes 10%；
Southeast anaerobe in summer is at each production process probability of happening:
The residual wine of secondary: Lactobacillus brevis 69%, Wei Si Salmonella 3%, Lin Denaishi lactobacillus 9%；
Bright beer tank rice wine liquid is entered: Lactobacillus brevis 59% after dilution；
Storage wine phase fermentation liquid: zymomonas mobilis 13%；
High bubble phase fermentation liquid: lactobacillus casei 10%；
Southeast aerobic bacteria in winter is at each production process probability of happening:
Finished wine: Bacillus coagulans 16%, plants raw Raoul bacterium 8%；
Sharp foam water: acetobacter aceti 72%；
The residual wine of secondary: bacillus amyloliquefaciens 80%, scytoblastema noy bacillus cereus 5%；
Aseptic water pot: bacillus subtilis 24%；
Southeast anaerobe in winter is at each production process probability of happening:
Finished wine: Lactobacillus brevis 19%, Lactobacillus plantarum 19%；
Rice wine after gland: Lactobacillus brevis 25%, Lactobacillus buchneri 25%；
The residual wine of secondary: megacoccus 56%, harmful sheet coccus 38%；
Bright beer tank rice wine liquid is entered: Lactobacillus brevis 63%, lactobacillus casei 13%, pantoea agglomerans 13% after dilution；
High bubble phase fermentation liquid: zymomonas mobilis 38%；
Northwest Summer and winter aerobic bacteria is at each production process probability of happening:
Bottle washer outlet empty bottle: Bacillus licheniformis 67%, Edwardsiella tarda 8%, bacillus cereus 7%；
Bright beer tank rice wine liquid is entered: Bacillus coagulans 25%, streptococcus thermophilus 45% after dilution；
Dilution oxygen remove exports deoxygenated water: Bacillus coagulans 5%, plants raw Raoul bacterium 40%；
Strainer outlet rice wine liquid: Bacillus licheniformis 72%；
CIP hot-water cylinder hot water: Bacillus coagulans 40%；
Softening water: Bacillus licheniformis 34%；
Northwest Summer and winter anaerobe is at each production process probability of happening:
Bottle washer outlet empty bottle: pantoea agglomerans 76%；
Bright beer tank rice wine liquid is entered: Lin Denaishi lactobacillus 32% after dilution；
Strainer outlet rice wine liquid: Lin Denaishi lactobacillus 49%；
Bright beer tank rice wine liquid is entered: Lin Denaishi lactobacillus 32% after dilution；
Strainer outlet rice wine liquid: Lin Denaishi lactobacillus 49%.
10. the system described in claim 4-9 is in the application identified or in auxiliary qualification brewery sludge.
CN201610265317.7A 2016-04-26 2016-04-26 System for identification or assisted identification of microorganisms in beer production process and application thereof Pending CN105950712A (en)

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Publication number Priority date Publication date Assignee Title
CN107034169A (en) * 2017-06-21 2017-08-11 湖南农业大学 A kind of enterobacteria 10 17 and its application in producing and ethanol
CN107034169B (en) * 2017-06-21 2019-09-03 湖南农业大学 A kind of enterobacteria 10-17 and its application in producing and ethanol
CN108827889A (en) * 2018-06-13 2018-11-16 江西中医药大学 A kind of glue class material discrimination method based on optical characteristics
CN109735466A (en) * 2019-01-25 2019-05-10 江苏大学 A kind of promotion low temperature, microorganism formulation of low-oxygen environment plant growth and preparation method thereof
CN110128568A (en) * 2019-05-21 2019-08-16 扬州日兴生物科技股份有限公司 A method of discarded shrimp and crab shells chitin extraction is handled using acetyl Exiguobacterium sp
CN110283745A (en) * 2019-06-27 2019-09-27 浙江工业大学 Hospital acinetobacter calcoaceticus FK2 and its application in degradable organic pollutant
CN110283745B (en) * 2019-06-27 2021-05-11 浙江工业大学 Acinetobacter hospital FK2 and application thereof in degrading organic pollutants
CN110804560A (en) * 2019-12-11 2020-02-18 自然资源部第三海洋研究所 Yeast 9-1 for producing cellulase and protease and application thereof, microbial preparation and preparation method and application thereof
WO2021120619A1 (en) * 2019-12-18 2021-06-24 江苏恒顺醋业股份有限公司 Bacillus safensis, microbial agent, and applications thereof and vinegar preparation method
CN113253782A (en) * 2021-05-26 2021-08-13 焦作大学 Adopt biochemical industry's of thing networking fermentation environment adjusting device
CN113253782B (en) * 2021-05-26 2022-11-22 青岛福圣愿生物科技有限公司 Adopt biochemical industry's of thing networking fermentation environment adjusting device
CN115938478A (en) * 2023-02-06 2023-04-07 中国医学科学院北京协和医院 System and method for predicting sensitivity of Klebsiella to amikacin

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