CN109729963A - A kind of nanometer siliceous deposits barrier heavy metal method based on rice material - Google Patents
A kind of nanometer siliceous deposits barrier heavy metal method based on rice material Download PDFInfo
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- CN109729963A CN109729963A CN201910102682.XA CN201910102682A CN109729963A CN 109729963 A CN109729963 A CN 109729963A CN 201910102682 A CN201910102682 A CN 201910102682A CN 109729963 A CN109729963 A CN 109729963A
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Abstract
The present invention provides a kind of, and the nanometer siliceous deposits based on rice material obstructs heavy metal method, comprising steps of the 1) vernalization of rice paddy seed, to rice root long to 5cm；2) with root system 4 hours containing immersion plant in the 0.5mmol/L-1mmol/L calcium chloride culture solution that concentration is 0.03g/L-0.05g/L polyethyleneimine；3) with the 0.5mmol/L-1mmol/L calcium chloride culture solution containing 10mmol/L-15mmol/L methyl orthosilicate, the root system of plant is impregnated 8 hours；4) it uses Solution culture method plant 12 hours.The nanometer siliceous deposits barrier heavy metal method of above-mentioned offer is the formation using PEI induction nano-silicon in root system surface deposition and nano silicon structure (nano-silicon shell), to alleviate murder by poisoning of the heavy metal to plant.The nano-silicon shell will not influence plant normal life, and facilitate the mechanism of action that researcher deeply understands nano silicon structure, improve heavy metal passivation Barrier Technology.
Description
Technical field
The invention belongs to agricultural plantation technology fields, and in particular to a kind of nanometer siliceous deposits barrier weight based on rice material
Metal process.
Background technique
With the development of industrialization, it is got worse with the application of a large amount of chemical fertilizer, soil acidification, and then causes heavy metal-polluted soil
Pollution problem seriously endangers crop production and the health of the mankind.Wherein, some researches show that by electrostatic interaction nanometer
Silicon materials deposit to the surface of solids, can form the anti-film of increasing of nanostructure binary collaboration in cell wall and space between cells to resist
Various environment-stress, to enable plant cell to abiotic stress such as heavy metal, arid, saline and alkaline, lodging, low temperature and high temperature etc.
Resistance significantly improves.However the prior art only obstructs in the level of cell to explore nano-silicon in cell surface Energy Deposition
Heavy metal enters in plant cell, but how to be applied in agricultural planting, also lacks means that are effective, being easy to carry out.
Summary of the invention
Aiming at the problem that cannot achieve plant in the prior art in planting process and obtain nano silicon material deposition, the present invention
A kind of nanometer siliceous deposits barrier heavy metal method based on rice material is provided, (PEI) is co-cultured by nano-silicon and is applied to
It carries out embedding root system on root system of plant, so that forming nano-silicon layer outside root system to protect plant.
Technical solution is as follows:
A kind of nanometer siliceous deposits barrier heavy metal method based on rice material, comprising steps of
1) vernalization of rice paddy seed, to rice root long to 5cm；
2) with containing the 0.5mmol/L-1mmol/L calcium chloride culture that concentration is 0.03g/L-0.05g/L polyethyleneimine
Root system 4 hours that plant is impregnated in liquid；
3) with the 0.5mmol/L-1mmol/L calcium chloride culture solution containing 10mmol/L-15mmol/L methyl orthosilicate, leaching
Root system 8 hours for steeping plant；
4) it uses Solution culture method plant 12 hours.
Further, step 2)~4) circulating repetition is at least three times.
Wherein, step 2)~4) it replaces 1~2 time clear is carried out to plant root with pure water before culture solution/nutrient solution every time
It washes.
Above-mentioned steps 1) described in vernalization step specifically include:
It 1-1) chooses full rice paddy seed to be sterilized 30 minutes with liquor natrii hypochloritis, be cleaned 5-6 times after disinfection；
Pure water wet pans 1-2) are used, layer overlay moisturizing medium is wet with pure water again, and rice paddy seed is equably put
It sets in container；
1-3) when the root long of rice paddy seed is to 1~2cm, seed is put into the plate of shearing hole, is placed on and fills nutrition
In the container of liquid, cultivate 3-4 days.
Further, step 1-3) in rice paddy seed be evenly placed upon culture 2~4 days in container.
Above-mentioned offer nanometer siliceous deposits barrier heavy metal method be using PEI induction nano-silicon root system surface deposition and
The formation of nano silicon structure (nano-silicon shell), to alleviate murder by poisoning of the heavy metal to plant.The nano-silicon shell will not influence plant
Normal life, and facilitate the mechanism of action that researcher deeply understands nano silicon structure, improve heavy metal passivation barrier
Technology.
Specific embodiment
It is clearly and completely described below with reference to technical effect of the embodiment to design and generation of the invention, with
It is completely understood by the purpose of the present invention, feature and effect.Obviously, described embodiment is that a part of the invention is implemented
Example, rather than whole embodiments, based on the embodiment of the present invention, those skilled in the art is not before making the creative labor
Other embodiments obtained are put, the scope of protection of the invention is belonged to.
Embodiment 1, nanometer siliceous deposits obstruct heavy metal method, specifically include step:
1) vernalization of rice paddy seed:
Full rice paddy seed 1-1) is chosen from spike of rice to be sterilized 30 minutes with liquor natrii hypochloritis, is cleaned after disinfection
5-6 times；
Wet with pure water 1-2) with a medium-sized turnover box, layer overlay paper handkerchief is wet with pure water again, by rice seed
Son is evenly placed upon in case, is cultivated 3 days or so；
1-3) when the root long of rice paddy seed is to 1-2cm, seed is put into the PCR plate of shearing hole, is placed on and fills battalion
On the small-sized turnover box of nutrient solution, cultivate 3-4 days, it is long to 5cm to rice；
2) with containing concentration be 0.03g/L polyethyleneimine (PEI) 0.5mmol/L calcium chloride culture solution in handle plant
Root system 4 hours, pure water cleaned plant root 1~2 time；
3) small with the 1mmol/L calcium chloride culture solution processing plant root 8 containing 10mmol/L methyl orthosilicate (TMOS)
When, pure water cleans plant root 1~2 time；
4) it uses Solution culture method 12 hours, pure water cleans plant root 1~2 time；
5) step 2)~4 are repeated) three times, complete embedding.
Embodiment 2, nanometer siliceous deposits obstruct heavy metal method, specifically include step:
1) vernalization of rice paddy seed:
Full rice paddy seed 1-1) is chosen from spike of rice to be sterilized 30 minutes with liquor natrii hypochloritis, is cleaned after disinfection
5-6 times；
Wet with pure water 1-2) with a medium-sized turnover box, layer overlay paper handkerchief is wet with pure water again, by rice seed
Son is evenly placed upon in case, is cultivated 3 days or so；
1-3) when the root long of rice paddy seed is to 1-2cm, seed is put into the PCR plate of shearing hole, is placed on and fills battalion
On the small-sized turnover box of nutrient solution, cultivate 3-4 days, it is long to 5cm to rice；
2) with containing concentration be 0.05g/L polyethyleneimine (PEI) 1mmol/L calcium chloride culture solution in handle plant root
It is 4 hours, pure water cleans plant root 1~2 time；
3) plant root 8 is handled with the 0.5mmol/L calcium chloride culture solution containing 15mmol/L methyl orthosilicate (TMOS)
Hour, pure water cleans plant root 1~2 time；
4) it uses Solution culture method 12 hours, pure water cleans plant root 1~2 time；
5) step 2)~4 are repeated) three times, complete embedding.
Embodiment 3, nanometer siliceous deposits obstruct heavy metal method, specifically include step:
1) vernalization of rice paddy seed:
Full rice paddy seed 1-1) is chosen from spike of rice to be sterilized 30 minutes with liquor natrii hypochloritis, is cleaned after disinfection
5-6 times；
Wet with pure water 1-2) with a medium-sized turnover box, layer overlay paper handkerchief is wet with pure water again, by rice seed
Son is evenly placed upon in case, is cultivated 3 days or so；
1-3) when the root long of rice paddy seed is to 1-2cm, seed is put into the PCR plate of shearing hole, is placed on and fills battalion
On the small-sized turnover box of nutrient solution, cultivate 3-4 days, it is long to 5cm to rice；
2) with containing concentration be 0.04g/L polyethyleneimine (PEI) 0.8mmol/L calcium chloride culture solution in handle plant
Root system 4 hours, pure water cleaned plant root 1~2 time；
3) plant root 8 is handled with the 0.5mmol/L calcium chloride culture solution containing 13mmol/L methyl orthosilicate (TMOS)
Hour, pure water cleans plant root 1~2 time；
4) it uses Solution culture method 12 hours, pure water cleans plant root 1~2 time.
Claims (5)
1. a kind of nanometer siliceous deposits based on rice material obstructs heavy metal method, it is characterised in that comprising steps of
1) vernalization of rice paddy seed, to rice root long to 5cm；
2) with containing in the 0.5mmol/L-1mmol/L calcium chloride culture solution that concentration is 0.03g/L-0.05g/L polyethyleneimine
Root system 4 hours for impregnating plant；
3) it with the 0.5mmol/L-1mmol/L calcium chloride culture solution containing 10mmol/L-15mmol/L methyl orthosilicate, impregnates and plants
Root system 8 hours of strain；
4) it uses Solution culture method plant 12 hours.
2. the nanometer siliceous deposits according to claim 1 based on rice material obstructs heavy metal method, which is characterized in that step
It is rapid 2)~4) circulating repetition is at least three times.
3. the nanometer siliceous deposits according to claim 2 based on rice material obstructs heavy metal method, which is characterized in that step
It is rapid 2)~4) replace culture solution/nutrient solution every time before 1~2 time cleaning is carried out to plant root with pure water.
4. the nanometer siliceous deposits according to claim 1 based on rice material obstructs heavy metal method, which is characterized in that step
Rapid 1) the described vernalization step specifically includes:
It 1-1) chooses full rice paddy seed to be sterilized 30 minutes with liquor natrii hypochloritis, be cleaned 5-6 times after disinfection；
Pure water wet pans 1-2) are used, layer overlay moisturizing medium is wet with pure water again, and rice paddy seed is evenly placed upon
In container；
1-3) when the root long of rice paddy seed is to 1~2cm, seed is put into the plate of shearing hole, is placed on and fills nutrient solution
In container, cultivate 3-4 days.
5. the nanometer siliceous deposits according to claim 4 based on rice material obstructs heavy metal method, which is characterized in that step
Rapid 1-3) in, rice paddy seed is evenly placed upon culture 2~4 days in container.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024174288A1 (en) * | 2023-02-21 | 2024-08-29 | 浙江大学 | Method for enhancing plant resistance to heavy metal chromium by silicon layer-by-layer self-assembly |
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| CN103789114A (en) * | 2013-12-26 | 2014-05-14 | 广东省生态环境与土壤研究所 | Selenium-doped nano silica sol used for producing selenium-enriched rice and capable of suppressing absorption and accumulation of heavy metals in paddy rice and preparation method for selenium-doped nano silica sol |
| CN107779426A (en) * | 2017-11-15 | 2018-03-09 | 佛山科学技术学院 | A kind of plant root edge cell coated Si processing method |
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2019
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| CN1907029A (en) * | 2006-08-09 | 2007-02-07 | 广东省生态环境与土壤研究所 | Rare earth composite silicasol capable of repressing rice absorbing high-density metal |
| CN103789114A (en) * | 2013-12-26 | 2014-05-14 | 广东省生态环境与土壤研究所 | Selenium-doped nano silica sol used for producing selenium-enriched rice and capable of suppressing absorption and accumulation of heavy metals in paddy rice and preparation method for selenium-doped nano silica sol |
| CN107779426A (en) * | 2017-11-15 | 2018-03-09 | 佛山科学技术学院 | A kind of plant root edge cell coated Si processing method |
| CN107828715A (en) * | 2017-11-15 | 2018-03-23 | 佛山科学技术学院 | A kind of plant root edge cell processing method for being used to deposit nano silicon |
| CN108040844A (en) * | 2017-11-15 | 2018-05-18 | 佛山科学技术学院 | A kind of aluminum-resistant poison pea seedling inoculation method |
| CN109156475A (en) * | 2018-08-20 | 2019-01-08 | 中国农业大学 | A kind of compound spray in pollution of heavy metals in rice blade face |
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| WO2024174288A1 (en) * | 2023-02-21 | 2024-08-29 | 浙江大学 | Method for enhancing plant resistance to heavy metal chromium by silicon layer-by-layer self-assembly |
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Application publication date: 20190510 |