CN109718345A - Prevent and treat Chinese medicine composition and preparation method that poison invades lung kidney type porcine reproductive and respiratory syndrome - Google Patents

Abstract

A kind of Chinese medicine composition for preventing and treating poison and invading lung kidney type porcine reproductive and respiratory syndrome of the present invention, Chinese medicine including following parts by weight meter: 20-35 parts of radix scrophulariae, Radix Ophiopogonis 25-45 part, 10-20 parts of radix rehmanniae recen, 25-45 parts of gypsum, 10-25 parts of the leaf of bamboo, 20-40 parts of the root of straight ladybell, 30-45 parts of radix polygonati officinalis, 10-20 parts of dendrobium nobile, 10-25 parts of the dried immature fruit of citron orange, 20-35 parts of rheum officinale, 25-40 parts of Cortex Magnoliae Officinalis, 10-30 parts of saltcake, 35-55 parts of Radix Isatidis, 25-35 parts of the coptis, 10-25 parts of bulbus fritillariae cirrhosae, 10-20 parts of the tuber of pinellia, 25-45 parts of cape jasmine, 20-40 parts of campanulaceae, 40-55 parts of Radix Glycyrrhizae, 15-30 parts of Charred Triplet, 25-45 parts of almond, 10-20 parts of loguat leaf, purple perilla 15-35 Part, 10-25 parts of birthwort.All medicines share, clearing heat and nourishing yin moisturize removing toxic substances, and nourishing the stomach to improve the production of body fluid, relieving stagnant Qi disperse accumulation, defaecation benefit diaphragm, stasis removing, promoting blood circulation, component compatibility is reasonable, treating both manifestation and root cause of disease, no recurrence, are suitable for large-scale application.

Description

Prevent and treat poison invade lung kidney type porcine reproductive and respiratory syndrome Chinese medicine composition and Preparation method

Technical field:

The invention belongs to veterinary drug technical fields, and in particular to one kind is for preventing and treating porcine reproductive and respiratory syndrome poison Invade the Chinese medicine composition and preparation method thereof of lung kidney type symptom.

Background technique:

Porcine reproductive and respiratory syndrome (Porcine reproductive and respiratory syndrome, PRRS) be pig caused by virus a kind of breeding difficulty and respiratory system infectious disease, the ear of part sick pig is blue, also known as " pig blue-ear disease ".It is characterized in that anorexia, fever, later stage of pregnancy are miscarried, stillborn foetus and the mummification of fetus are produced；Young age piglet exhales Desorption system disease and mortality.Clinical symptom is obvious after piglet is infected with 2~28 ages in days, and the death rate is up to 80%.Farrowing of premature pig It is dead at that time or in several days in birth, most of birth piglets performance expiratory dyspnea, muscular tremor, hindlimb paralysis, incoordination, Sneezing, drowsiness, some piglet ears are blue and body end skin cyanosis.Conjunctivitis and abdomen occur for bred pigs eyes swelling It rushes down, and pneumonia occurs.Showed after boar infection cough, sneeze, it is spiritual it is depressed, loss of appetite, be short of breath and dyskinesia, property Be intended to weaken, semen quality decline, ejaculation amount it is few.

According to sow gestation deuterogenesis miscarriage, the newborn piglet death rate is high and clinical symptom and interstitial pneumonia etc. can Tentatively make diagnosis.But (porcine parvovirus, pig puppet are mad since PRRS not only causes the disease of pig breeding dysfunction with other many Dog disease, smoothing filter operator, Japanese B encephalitis, swine fever etc.) clinical symptom it is closely similar, and be easily mixed infection, Clinical symptom has very big difference again after different pig farms infection PRRS simultaneously, therefore makes a definite diagnosis and depend on laboratory diagnosis.It is built at present It founds a variety of RT-PCR methods for increasing PRRSV gene more, and is widely used to face and examines detection.American type can be also distinguished by the method With Europe class strain.It should be noted that in diagnosis by this disease and pig parvoviral, porcine pseudorabies, encephalitis B and Delayed onset swine fever Identify Deng phase.

This disease there is no specific medicament therapy at present, mainly take comprehensive measures for prevention and control and symptomatic treatment.Due to using at present Antibiotic medicine therapy, there are the risk of antibiotic residue, therefore using the treatment pig breeding of the Chinese medicine of natural noresidue and exhale Inhaling syndrome becomes current mainstream therapeutic modality.

Summary of the invention:

Since swinery infection porcine reproductive and respiratory syndrome will appear different symptoms, wherein there is high fever, spirit is depressed, There is purple dot in expiratory dyspnea, rubefaction, anorexia, appetite stimulator, have sharp ears and four limbs, and part sow is miscarried, and produce dead Tire, the mummification of fetus, in Chinese medical discrimination heat symptoms caused by an exopathgen enter, heap soil or fertilizer over and around the roots closes lung qi, makes impairment of dispersing and descending function of the lung, Lung Qi upward reversal and see expiratory dyspnea or rapid, Belong to poison and invades lung kidney type symptom.

There are problems that medicament residue antibiotic, the present invention provides one kind and invades lung kidney type pig for preventing and treating poison The Chinese medicine composition and preparation method of reproductive and respiratory syndrome.

The present invention solves scheme used by its technical problem: one kind invading the breeding of lung kidney type pig for preventing and treating poison With the Chinese medicine composition of respiration syndrome, the Chinese medicine including following parts by weight meter: 20-35 parts of radix scrophulariae, Radix Ophiopogonis 25-45 part, Radix Rehmanniae It is 10-20 parts yellow, 25-45 parts of gypsum, 10-25 parts of the leaf of bamboo, 20-40 parts of the root of straight ladybell, 30-45 parts of radix polygonati officinalis, 10-20 parts of dendrobium nobile, the dried immature fruit of citron orange 10-25 parts, 20-35 parts of rheum officinale, 25-40 parts of Cortex Magnoliae Officinalis, 10-30 parts of saltcake, 35-55 parts of Radix Isatidis, 25-35 parts of the coptis, bulbus fritillariae cirrhosae 10-25 parts, 10-20 parts of the tuber of pinellia, 25-45 parts of cape jasmine, 20-40 parts of campanulaceae, 40-55 parts of Radix Glycyrrhizae, 15-30 parts of Charred Triplet, almond 25- 45 parts, 10-20 parts of loguat leaf, 15-35 parts of purple perilla, 10-25 parts of birthwort.

A kind of above-mentioned Chinese medicine composition that lung kidney type porcine reproductive and respiratory syndrome is invaded for preventing and treating poison, including The Chinese medicine of following parts by weight meter: 25-35 parts of radix scrophulariae, Radix Ophiopogonis 30-45 part, 15-20 parts of radix rehmanniae recen, 35-45 parts of gypsum, leaf of bamboo 20- 25 parts, 30-40 parts of the root of straight ladybell, 30-40 parts of radix polygonati officinalis, 10-15 parts of dendrobium nobile, 10-15 parts of the dried immature fruit of citron orange, 20-30 parts of rheum officinale, Cortex Magnoliae Officinalis 30-40 Part, 10-15 parts of saltcake, 45-55 parts of Radix Isatidis, 30-35 parts of the coptis, 15-25 parts of bulbus fritillariae cirrhosae, 10-15 parts of the tuber of pinellia, cape jasmine 35-45 Part, 20-30 parts of campanulaceae, 40-50 parts of Radix Glycyrrhizae, 15-20 parts of Charred Triplet, 35-45 parts of almond, 10-15 parts of loguat leaf, purple perilla 15-25 Part, 20-25 parts of birthwort.

A kind of above-mentioned Chinese medicine composition that lung kidney type porcine reproductive and respiratory syndrome is invaded for preventing and treating poison, including The Chinese medicine of following parts by weight meter: 30 parts of radix scrophulariae, 40 parts of Radix Ophiopogonis, 15 parts of radix rehmanniae recen, 40 parts of gypsum, 20 parts of the leaf of bamboo, 30 parts of the root of straight ladybell, beautiful 35 parts of bamboo, 15 parts of dendrobium nobile, 15 parts of the dried immature fruit of citron orange, 30 parts of rheum officinale, 40 parts of Cortex Magnoliae Officinalis, 15 parts of saltcake, 45 parts of Radix Isatidis, 30 parts of the coptis, river 20 parts of fritillaria, 15 parts of the tuber of pinellia, 35 parts of cape jasmine, 25 parts of campanulaceae, 45 parts of Radix Glycyrrhizae, 20 parts of Charred Triplet, 40 parts of almond, 15 parts of loguat leaf, 20 parts of purple perilla, 20 parts of birthwort.

A kind of preparation method for invading the Chinese medicine composition of lung kidney type porcine reproductive and respiratory syndrome for preventing and treating poison, The following steps are included: weighing each Chinese medicine according to weight part ratio, put it into medicine decocting tank, the pure water of 10 times of amounts is added into tank Or 70% ethyl alcohol impregnate 1h, decoct 30min, filtering, obtain for the first time decoct filtrate；The pure of 10 times of amounts is added into filter residue Water or 70% ethyl alcohol decoct 30min, filter to obtain second of decoction filtrate；Merge filtrate twice, is concentrated under reduced pressure into medicinal extract, dry spray At powder；Gained filter residue dries pulverizing is uniformly mixed with powder obtained by filtrate, packing pack.

Chinese medicine composition of the present invention, radix scrophulariae in side is sweet, bitter, salty, trembles with fear, and enters lung, stomach, kidney channel, this product can heat-clearing rush down Fire, and yin-fluid can be nourished, giving consideration to both the incidental and fundamental, no matter heat toxin excess fire, yin asthenia generating intrinsic heat can be used；

Radix Ophiopogonis, sweet, slight bitter is cool, enters lung, stomach, the heart channel of Hang-Shaoyin, this product clearing heat and nourishing yin, moistening lung to arrest cough, for yin asthenia generating intrinsic heat, dry cough Few phlegm, nourishing the stomach to improve the production of body fluid are suitable for yin asthenia generating intrinsic heat, consumption of body fluid caused by febrile disease, thirsty greedy drink, dry constipation of intestines；

Radix rehmanniae recen, it is sweet, bitter, cool, enter the heart, kidney channel, clearing heat and cooling blood, nourishing Yin and promoting production of body fluid, for preventing pyreticosis and polydipsia, in the deficiency of Yin Heat；

Gypsum, pungent, sweet, Great Cold enter lung, and stomach meridian, clearing heat-fire, this product Great Cold has powerful clearing heat-fire effect, kind Clean air divides real heat, is used for the big heat of lung stomach, the real heats such as hyperpyrexia Kang Sheng card, and eliminating heat from the lung is used for cough with lung heat, and asthma is thirsty The excessive heat syndromes such as greedy drink, let out stomach energy, for the card such as stomach fire Kang Sheng；

The leaf of bamboo, it is sweet, it is light, it trembles with fear, enters the heart, stomach, small intestinl channel, heat-clearing, diuresis is damp and hot for the heart channel of Hang-Shaoyin, and aphthae, urine is short red, Clearing away the stomach-heat, with controlling stomach energy；

The root of straight ladybell, it is sweet, it is cool, enter lung, stomach meridian, moistening lung to arrest cough, nourishing the stomach to improve the production of body fluid, yin-nourishing, eliminating the phlegm, the clearing lung-heat of this product energy is hot, lung yin is supported, QI invigorating eliminating the phlegm lacks phlegm for the chronic cough deficiency syndrome of the lung and thermal burn lung yin dry cough, nourishing stomach-YIN, can be used for after pyreticosis or prolonged illness impairment of yin caused by mouth Dry tongue is dry, constipation, the red rapid pulse of tongue；

Radix polygonati officinalis, sweet and neutral enter lung, and stomach meridian, nourishing Yin and moistening lung, nourishing the stomach to improve the production of body fluid, clearing heat and promoting fluid, scorching suitable for lung stomach, yin-fluid is not Thirsty greedy drink, dryness of the lung dry cough caused by foot；

Dendrobium nobile, it is sweet, it is slightly cold, enter lung, stomach, kidney channel, Yin-nourishing and body fluid promoting, heat-clearing nourishing the stomach, the yin of lung and stomach nourishing and clearind deficient heat are applicable in In consumption of YIN caused by febrile disease, the few thirsty or deficiency of Yin of saliva, heat does not move back person, lung long, and stomach has heat, and thirsty greedy drink person promotes gastric secretion, helps to disappear Change；

The dried immature fruit of citron orange, it is bitter, it is slightly cold, enter spleen, stomach meridian, relieving stagnant Qi disperse accumulation, defaecation benefit diaphragm, with the taste stagnation of the circulation of vital energy is controlled, phlegm wet water is drunk caused Tripe distention and fullness in the abdomen can enhance stomach, intestines rhythmicity is wriggled with constipation with heat retention, tripe distention and fullness in the abdomen pain person is controlled, and be conducive to excrement and gas Discharge；

Rheum officinale, it is bitter, it trembles with fear, enters spleen, stomach, large intestine, liver, pericardium channel, stasis removing, purging fire and cooling blood, promoting blood circulation, this product is good at Cleanse sthenia gastropyrexia, constipation is stagnant, for the key medicine that bitter cold is captured, with control constipation with heat retention, abdominal pain rises and retires, real heat stop up it is stagnant, under can rushing down, It can expel the heat-evil again, promoting blood circulation is used for stagnation of blood stasis, and Qinghua is damp and hot, clearing heat and detoxicating；

Cortex Magnoliae Officinalis, it is bitter, it is acrid in flavour and warm in nature, enter spleen, stomach, large intestine channel, promoting qi circulation to remove dampness, relieving asthma, this product can be except stomach and intestine stagnant gas, eliminating dampness Spleen is transported, with controlling damp retention in middle-jiao, tripe distention and fullness in the abdomen, abdominal pain or the hiccup caused by the stagnation of the circulation of vital energy is unfavorable；

Saltcake, bitter, salty, Great Cold enter stomach, and large intestine channel, under softening hard masses are rushed down, clearing heat-fire, stagnant suitable for real heat, excrement is dry Knot, tripe distention and fullness in the abdomen, clearing heat-fire, disinfection detumescence, with controlling red eye, swell pain caused by heat toxin, canker sore and skin sore swells；

Radix Isatidis, bitter, cold, return heart, stomach meridian are clearing heat and detoxicating, cool blood relieving sore-throat, are apt to removing summer-heat real heat fire toxin, and heat-clearing and cool blood are used Control maculopapulae due to toxic heat；

The coptis, it is bitter, it trembles with fear, enters the heart, liver, stomach, large intestine channel, heat-clearing and damp-drying drug, purging fire for removing toxin, this product is heat-clearing and damp-drying drug key medicine, all Belong to damp and hot all cards, can apply, especially most suitable with the card of stomach stagnation of damp-heat, clearing heat-fire effect is stronger, with controlling heart-fire hyperactivity, mouth Tongue gives birth to sore, and three Jiao Jire control the hot tongue boil of the heart, are good at clearing heat and detoxicating；

Bulbus fritillariae cirrhosae, it is bitter, it is sweet, it is slightly cold, enter the heart, lung channel is relieving cough and reducing sputum, and heat-clearing dissipating bind controls phlegm and coughs less, the deficiency of Yin or dryness of the lung cough It coughs, affection of exogenous wind-heat, convulsive seizure due to phlegm-fire pent-up；

The tuber of pinellia, acrid in flavour and warm in nature, returns spleen, stomach, lung channel is eliminating dampness and eliminating phlegm, stopping nausea and vomiting by lowering the adverse flow of QI, and help digestion dissipating bind, the pungent scattered temperate-dryness of this product, drops inverse The function of preventing or arresting vomiting is significant, can be used for regurgitation and emesis, abdominal distension, is especially suitable for removing dampness and eliminating phlegm to vomiting caused by drink and damp evil retardance is stopped, For the key medicine for controlling damp phlegm cough and asthma, it is suitable for cough with dyspnea, sputum is stopped up stagnant, help digestion dissipating bind, with controlling tripe distention and fullness in the abdomen；

Cape jasmine, bitter, cold, return heart, lung, tri-jiao channel, purging fire for removing toxin, reducing fever and causing diuresis, cool blood, hemostasis, this product have purging fire for removing toxin Effect, it is kind clear away heart-fire, liver, tri-jiao channel heat, it is burning hot through it to be longer than clearing liver especially, clear three burnt fire and diuresis, and benefit liver and gallbladder damp-heat, cool blood Hemostasis, suitable for hematuria caused by blood-heat bleeding, hematochezia；

Campanulaceae, it is bitter, it is pungent, it puts down, distributed in lung channel, promoting the dispersing function of the lung, eliminating the phlegm, relieving sore-throat, apocenosis, this product clearing lung and eliminating the phlegm is longer than promoting the dispersing function of the lung and evacuates Ailment said due to cold or exposure, to control coughing with a lot of sputum caused by affection of exogenous wind-cold or wind-heat, abscess of throat, with controlling lung heat cough and asthma, apocenosis detumescence, with controlling lung Carbuncle, sore are not burst, moreover it is possible to be opened and be mentioned lung qi, dredge stomach and intestine；

Radix Glycyrrhizae, sweet and neutral enter 12 warps, and tonifying middle-Jiao and Qi is clearing heat and detoxicating, moistening lung to arrest cough, mitigates pharmacological property, has sweet slow moistening lung to stop The function of cough is wheezed with cough is controlled, lung cold cough and asthma or lung heat cough and asthma, can mitigate the high strong property of drug, have the function of coordinating the drug actions of a prescription；

Charred Triplet, i.e. melanoidin malt, charred FRUCTUS CRATAEGI, stir-baked MASSA FERMENTATA MEDICI-NALIS, this three tastes medicine have the stagnant function of good disperse accumulationization, and melanoidin malt has The effect of good digestible starch group food；Charred FRUCTUS CRATAEGI is good at treating meat or greasy excessive caused food stagnation；Stir-baked MASSA FERMENTATA MEDICI-NALIS is then sharp In digestion rice and flour food, three medicines are shared, and can significantly enhance digestive function；

Almond, bitter, temperature enters lung, and large intestine channel is relieving cough and asthma, relax bowel and defecation, bitter drop-down gas, and it is inverse to be mainly used for cough, syndrome characterized by dyspnea, Lung heat asthma, moisturizing dryness and lubricating intestine, dry constipation of intestines and puerperal constipation；

Loguat leaf, it is bitter, it puts down, enters lung, stomach meridian, this product please let out lung heat, and preventing phlegm from forming and stopping coughing is usually used in lung heat cough and asthma, with controlling the dryness of the lung Cough, clearing away the stomach-heat, preventing or arresting vomiting is inverse, and it is thirsty to control stomach energy；

Purple perilla, it is acrid in flavour and warm in nature, enter lung, the spleen channel delivers cold dispelling, relieving QI stagnancy in the stomach, this product energy sweating cold dispelling, open the inhibited lung-energy, sweating power Relatively strong, this product fragrant odour, energy promoting the circulation of qi is amusing, for tripe distention and fullness in the abdomen caused by the taste stagnation of the circulation of vital energy, loss of appetite, vomiting；

Birthwort, bitter, micro-pungent trembles with fear, enters lung, and large intestine channel, clearing lung-heat sending down abnormally ascending is relieving cough and asthma, this product clearing lung-heat sending down abnormally ascending, and cough-relieving is flat Asthma, is usually used in cough with lung heat, and abundant expectoration syndrome characterized by dyspnea has breathing with cough for heat with the deficiency syndrome of the lung is controlled, controls xeropulmonary cough, nose rhinorrhea.

All medicines share has clearing heat and nourishing yin, moisturizes removing toxic substances, nourishing the stomach to improve the production of body fluid, relieving stagnant Qi disperse accumulation, defaecation benefit diaphragm, stasis removing, work Blood and dissolving stasis, promoting qi circulation to remove dampness, relieving asthma, under softening hard masses are rushed down, cool blood relieving sore-throat is relieving cough and reducing sputum, and purging intense heat diuresis is relieving cough and asthma, and ease constipation is logical Just, the effect of bitter drop-down gas, harmonizing stomach and lowering adverse Qi, deliver cold dispelling, relieving QI stagnancy in the stomach, clearing lung and eliminating the phlegm, clearing lung-heat sending down abnormally ascending, clearing away fire and eliminating phlegm.

Specific embodiment:

Below with reference to embodiment, the invention will be further described.

Embodiment 1: for preventing and treating the Chinese medicine composition of porcine reproductive and respiratory syndrome, the medicine described in the present embodiment Lung kidney type symptom, the Chinese medicine including following parts by weight meter: radix scrophulariae 30g, Radix Ophiopogonis are invaded for treating porcine reproductive and respiratory syndrome poison 40g, radix rehmanniae recen 15g, gypsum 40 g, leaf of bamboo 20g, the root of straight ladybell 30g, radix polygonati officinalis 35g, dendrobium nobile 15g, dried immature fruit of citron orange 15g, 30 g of rheum officinale, Cortex Magnoliae Officinalis 40g, saltcake 15g, Radix Isatidis 45g, coptis 30g, bulbus fritillariae cirrhosae 20g, tuber of pinellia 15g, cape jasmine 35g, campanulaceae 25g, Radix Glycyrrhizae 45g, coke three Celestial 20g, almond 40g, loguat leaf 15g, purple perilla 20g, birthwort 20g.

Preparation method: accurately weighing above-mentioned each Chinese medicine, put it into medicine decocting tank, and the pure water of 10 times of amounts is added into tank 1h is impregnated, 30min is decocted, filtering obtains and decocts filtrate for the first time；The pure water that 10 times of amounts are added into filter residue decocts 30min, Filter to obtain second of decoction filtrate；Merge filtrate twice, is concentrated under reduced pressure into medicinal extract, is dried in vacuo into powder；The drying of gained filter residue It crushes, is uniformly mixed with powder obtained by filtrate, packing pack.

Embodiment 2: for preventing and treating the Chinese medicine composition of porcine reproductive and respiratory syndrome, the medicine described in the present embodiment Lung kidney type symptom, the Chinese medicine including following parts by weight meter: radix scrophulariae 30g, Radix Ophiopogonis are invaded for treating porcine reproductive and respiratory syndrome poison 40g, radix rehmanniae recen 18g, gypsum 40 g, leaf of bamboo 23g, the root of straight ladybell 35g, radix polygonati officinalis 35g, dendrobium nobile 12g, dried immature fruit of citron orange 13g, rheum officinale 25g, Cortex Magnoliae Officinalis 35g, saltcake 13g, Radix Isatidis 50g, coptis 32g, bulbus fritillariae cirrhosae 20g, the tuber of pinellia 12 g, cape jasmine 40g, campanulaceae 25g, Radix Glycyrrhizae 45g, coke three Celestial 18g, almond 40g, loguat leaf 13g, purple perilla 20g, birthwort 22g.

Preparation method: accurately weighing above-mentioned each Chinese medicine, put it into medicine decocting tank, and the volume point of 10 times of amounts is added into tank Number is that 70% ethyl alcohol impregnates 1h, decocts 30min, and filtering obtains and decocts filtrate for the first time；The volume of 10 times of amounts is added into filter residue Score is that 70% ethyl alcohol decocts 30min, filters to obtain second of decoction filtrate；Merge filtrate twice, is concentrated under reduced pressure into medicinal extract, vacuum It is dried to powder；Gained filter residue dries pulverizing is uniformly mixed with powder obtained by filtrate, packing pack.

Embodiment 3: for preventing and treating the Chinese medicine composition of porcine reproductive and respiratory syndrome, the medicine described in the present embodiment Lung kidney type symptom, the Chinese medicine including following parts by weight meter: radix scrophulariae 20g, Radix Ophiopogonis are invaded for treating porcine reproductive and respiratory syndrome poison 45g, radix rehmanniae recen 15g, gypsum 25g, leaf of bamboo 20g, the root of straight ladybell 30g, radix polygonati officinalis 30g, dendrobium nobile 10g, dried immature fruit of citron orange 10g, rheum officinale 20g, Cortex Magnoliae Officinalis 40g, saltcake 15g, Radix Isatidis 35g, coptis 30g, bulbus fritillariae cirrhosae 10g, tuber of pinellia 15g, cape jasmine 25g, campanulaceae 30g, Radix Glycyrrhizae 40g, coke three Celestial 15g, almond 25g, loguat leaf 15g, purple perilla 15g, birthwort 10g.

Preparation method: accurately weighing above-mentioned each Chinese medicine, each Chinese medicine crushed one by one, 400 meshes is crossed respectively, by all powder End is uniformly mixed up to Chinese medicine composition powder of the present invention.

Embodiment 4: for preventing and treating the Chinese medicine composition of porcine reproductive and respiratory syndrome, the medicine described in the present embodiment Lung kidney type symptom, the Chinese medicine including following parts by weight meter: radix scrophulariae 35g, Radix Ophiopogonis are invaded for treating porcine reproductive and respiratory syndrome poison 25g, radix rehmanniae recen 10g, gypsum 35g, leaf of bamboo 10g, the root of straight ladybell 20g, radix polygonati officinalis 45g, dendrobium nobile 20g, dried immature fruit of citron orange 15g, rheum officinale 30g, Cortex Magnoliae Officinalis 25g, saltcake 10g, Radix Isatidis 45g, coptis 35g, bulbus fritillariae cirrhosae 15g, tuber of pinellia 10g, cape jasmine 45g, campanulaceae 20g, Radix Glycyrrhizae 55g, coke three Celestial 30g, almond 35g, loguat leaf 10g, purple perilla 35g, birthwort 20g.

Preparation method: accurately weighing above-mentioned each Chinese medicine, put it into medicine decocting tank, and the pure water of 10 times of amounts is added into tank 1h is impregnated, 30min is decocted, filtering obtains and decocts filtrate for the first time；The volume fraction that 10 times of amounts are added into filter residue is 70% second Alcohol decocts 30min, filters to obtain second of decoction filtrate；Merge filtrate twice, is concentrated under reduced pressure into medicinal extract, is dried in vacuo into powder； Gained filter residue dries pulverizing is uniformly mixed with powder obtained by filtrate, packing pack.

Embodiment 5: for preventing and treating the Chinese medicine composition of porcine reproductive and respiratory syndrome, the medicine described in the present embodiment Lung kidney type symptom, the Chinese medicine including following parts by weight meter: radix scrophulariae 25g, Radix Ophiopogonis are invaded for treating porcine reproductive and respiratory syndrome poison 30g, radix rehmanniae recen 20g, gypsum 45g, leaf of bamboo 25g, the root of straight ladybell 40g, radix polygonati officinalis 40g, dendrobium nobile 15g, dried immature fruit of citron orange 25g, rheum officinale 35g, Cortex Magnoliae Officinalis 30g, saltcake 30g, Radix Isatidis 55g, coptis 25g, bulbus fritillariae cirrhosae 25g, tuber of pinellia 20g, cape jasmine 35g, campanulaceae 40g, Radix Glycyrrhizae 50g, coke three Celestial 20g, almond 45g, loguat leaf 20g, purple perilla 25g, birthwort 25g.

Preparation method: accurately weighing above-mentioned each Chinese medicine, put it into medicine decocting tank, and the volume point of 10 times of amounts is added into tank Number is that 70% ethyl alcohol impregnates 1h, decocts 30min, and filtering obtains and decocts filtrate for the first time；The pure of 10 times of amounts is added into filter residue Water decocts 30min, filters to obtain second of decoction filtrate；Merge filtrate twice, is concentrated under reduced pressure into medicinal extract, is dried in vacuo into powder； Gained filter residue dries pulverizing is uniformly mixed with powder obtained by filtrate, packing pack.

Embodiment 6: effect of drugs measurement

(1) the traditional chinese medicine composition of the invention is to Marc-145 cytotoxicity assay

96 porocyte culture plates of Marc-145 cell monolayer are covered in preparation, and Chinese medicine composition of the invention is cultivated with DMEM After base is diluted according to multiple proportions method, make drug it is final concentration of (500g/mL, 250g/mL, 125g/mL, 62.5g/mL, 31.25g/mL、15.63g/mL、 7.81g/mL、3.95g/mL、1.98g/mL、0.99g/mL、0.49g/mL、0.25g/mL、 0.12g/mL、0.06g/mL、0.03g/mL、15.43mg/mL、7.71mg/mL、3.86 mg/mL、1.93mg/mL、0.97mg/ ML, 0.48mg/mL, 0.24mg/mL), it is added separately in cell hole, each drug concentration does 4 repetitions, in 5%CO₂、37 48h is cultivated under the conditions of DEG C, 96 orifice plates are taken out after 48h, and 20 μ L MTT solution (5 mg/mL) are added in every hole, continue to take after cultivating 8h Culture plate out is inhaled and abandons culture solution in hole, and 150 μ L dimethyl sulfoxides are added to every hole, sets low speed on shaking table and shakes 5min, OD_570nmUnder the conditions of measure the light absorption value in each hole, then calculate the maximum safe concentration of drug；

(2) measurement of the traditional chinese medicine composition of the invention to PRRSV inhibitory effect

The traditional chinese medicine composition of the invention is diluted according to coubling dilution, make drug it is final concentration of (500g/mL, 250g/mL、125g/mL、62.5g/mL、31.25g/mL、15.63 g/mL、7.81g/mL、3.95g/mL、1.98g/mL、 0.99g/mL、0.49g/mL、 0.25g/mL、0.12g/mL、0.06g/mL、0.03g/mL、15.43mg/mL、7.71mg/mL、 3.86mg/mL, 1.93mg/mL, 0.97mg/mL, 0.48mg/mL, 0.24mg/mL), according to 4 repetitions of each concentration, press respectively 100 μ L medical fluids are accessed according to every hole, access has been covered in 96 porocyte culture plates of single layer Marc-145 cell, 5%CO₂, 37 DEG C 1h is cultivated, medical fluid is abandoned；Add TCID₅₀Virus liquid every hole 100 μ L, 5%CO₂, 37 DEG C of culture 1h separately set cell controls and virus Control.5%CO₂, 37 DEG C of culture 5d, daily observation cell situation.The traditional chinese medicine composition of the invention is calculated on Marc-145 cell Concentration is blocked to the absorption of virus；

(3) the traditional chinese medicine composition of the invention is on Marc-145 cell to the inhibiting effect of virus

It uses and has covered with 96 porocyte plates of single layer Marc-145 cell as culture plate, according to 100 holes μ L/ by TCID₅₀Disease Venom accesses on culture plate, 5%CO₂, 37 DEG C of culture 1h, inhale abandon virus liquid, cleaned 3 times with PBS liquid, add doubling dilution Medical fluid, make drug it is final concentration of (500g/mL, 250g/mL, 125g/mL, 62.5g/mL, 31.25g/mL, 15.63g/mL, 7.81g/mL、3.95g/mL、1.98g/mL、0.99g/mL、0.49g/mL、 0.25g/mL、0.12g/mL、0.06g/mL、 0.03g/mL、15.43mg/mL、7.71mg/mL、 3.86mg/mL、1.93mg/mL、0.97mg/mL、0.48mg/mL、 0.24mg/mL), according to 100 holes μ L/, each concentration is repeated 4 times, if cell controls group and virus control group.It will be inoculated thin Born of the same parents' culture plate is put into 5%CO₂, 37 DEG C of culture 5d, daily observation cell situation；The traditional chinese medicine composition of the invention is calculated in Marc-145 To the inhibition concentration of virus on cell.

(4) direct deactivation of the traditional chinese medicine composition of the invention on Marc-145 cell to virus

By TCID₅₀The virus liquid of concentration and the medical fluid of doubling dilution mix in equal volume, make the final concentration of (500g/ of drug mL、250g/mL、125g/mL、62.5g/mL、31.25g/mL、 15.63g/mL、7.81g/mL、3.95g/mL、1.98g/mL、 0.99g/mL、0.49g/mL、 0.25g/mL、0.12g/mL、0.06g/mL、0.03g/mL、15.43mg/mL、7.71mg/mL、 3.86mg/mL, 1.93mg/mL, 0.97mg/mL, 0.48mg/mL, 0.24mg/mL), 5%CO₂, 37 DEG C of culture 1h press respectively According to every 100 μ L of hole, each hole is repeated 4 times, and is inoculated into 96 orifice plates for covering with Marc-145 cell monolayer, while virus control is arranged Hole, drug control wells and blanc cell control, 5%CO₂, 37 DEG C of culture 5d, daily observation cell situation；Calculate Chinese medicine of the present invention Composition is on Marc-145 cell to the direct inactivation concentration of virus.

Measurement result is shown in Table 1.

The effect measuring result of 1 the traditional chinese medicine composition of the invention of table

As shown in Table 1, the traditional chinese medicine composition of the invention is 125g/ml to the maximum safe concentration of Marc-145 cell, i.e., in Drug composition concentration can carry out cell experiment, not have toxicity to cell in 125g/ml or less；Chinese medicine group through the invention Measurement of the object to PRRSV inhibitory effect is closed, the traditional chinese medicine composition of the invention blocks PRRSV that lesion does not occur to Marc-145 cell Cmin is 125g/ml；Measurement of the Chinese medicine composition on Marc-145 cell to the inhibiting effect of virus through the invention, It is 0.97mg/ml to the Cmin that lesion does not occur for Marc-145 cell that the traditional chinese medicine composition of the invention, which completely inhibits PRRSV,； Chinese medicine composition measures the direct deactivation of virus on Marc-145 cell through the invention, the traditional chinese medicine composition of the invention Complete inactivation PRRSV is 1.93mg/ml to the Cmin that lesion does not occur for Marc-145 cell.

Embodiment 7: clinical application effect

In September, 2018, Shandong profession raiser, 500 bull of livestock on hand sow, wherein 20 sow morbidities, main to show For high fever, spirit is depressed, expiratory dyspnea, and body temperature is increased to 41 DEG C, some rubefactions, anorexia, appetite stimulator, have sharp ears and four There is purple dot in limb, and part sow is miscarried, and produces stillborn foetus, and the mummification of fetus is diagnosed as according to clinical symptoms and molecular diagnosis Pig blue-ear disease belongs to poison and invades lung kidney type, is watered 5 tons with the traditional chinese medicine composition of the invention 1kg, twice a day, 5d is used in conjunction, and makes pig free It drinks；Symptom disappears after 4 days, and swinery restores normal.

The above be preferred embodiments of the invention, all equivalent variationss done according to scope of the present invention patent with repair Decorations, all should belong to protection scope of the present invention.

Claims (4)

1. a kind of Chinese medicine composition for invading lung kidney type porcine reproductive and respiratory syndrome for preventing and treating poison, which is characterized in that Chinese medicine including following parts by weight meter: 20-35 parts of radix scrophulariae, Radix Ophiopogonis 25-45 part, 10-20 parts of radix rehmanniae recen, 25-45 parts of gypsum, the leaf of bamboo 10-25 parts, 20-40 parts of the root of straight ladybell, 30-45 parts of radix polygonati officinalis, 10-20 parts of dendrobium nobile, 10-25 parts of the dried immature fruit of citron orange, 20-35 parts of rheum officinale, Cortex Magnoliae Officinalis 25-40 Part, 10-30 parts of saltcake, 35-55 parts of Radix Isatidis, 25-35 parts of the coptis, 10-25 parts of bulbus fritillariae cirrhosae, 10-20 parts of the tuber of pinellia, cape jasmine 25-45 Part, 20-40 parts of campanulaceae, 40-55 parts of Radix Glycyrrhizae, 15-30 parts of Charred Triplet, 25-45 parts of almond, 10-20 parts of loguat leaf, purple perilla 15-35 Part, 10-25 parts of birthwort.

2. a kind of Chinese medicine for invading lung kidney type porcine reproductive and respiratory syndrome for preventing and treating poison according to claim 1 Composition, which is characterized in that the Chinese medicine including following parts by weight meter: 25-35 parts of radix scrophulariae, Radix Ophiopogonis 30-45 part, radix rehmanniae recen 15-20 Part, 35-45 parts of gypsum, 20-25 parts of the leaf of bamboo, 30-40 parts of the root of straight ladybell, 30-40 parts of radix polygonati officinalis, 10-15 parts of dendrobium nobile, 10-15 parts of the dried immature fruit of citron orange, greatly It is 20-30 parts yellow, 30-40 parts of Cortex Magnoliae Officinalis, 10-15 parts of saltcake, 45-55 parts of Radix Isatidis, 30-35 parts of the coptis, 15-25 parts of bulbus fritillariae cirrhosae, half Summer 10-15 part, 35-45 parts of cape jasmine, 20-30 parts of campanulaceae, 40-50 parts of Radix Glycyrrhizae, 15-20 parts of Charred Triplet, 35-45 parts of almond, loquat 10-15 parts of leaf, 15-25 parts of purple perilla, 20-25 parts of birthwort.

3. a kind of Chinese medicine for invading lung kidney type porcine reproductive and respiratory syndrome for preventing and treating poison according to claim 1 Composition, which is characterized in that the Chinese medicine including following parts by weight meter: 30 parts of radix scrophulariae, 40 parts of Radix Ophiopogonis, 15 parts of radix rehmanniae recen, gypsum 40 Part, 20 parts of the leaf of bamboo, 30 parts of the root of straight ladybell, 35 parts of radix polygonati officinalis, 15 parts of dendrobium nobile, 15 parts of the dried immature fruit of citron orange, 30 parts of rheum officinale, 40 parts of Cortex Magnoliae Officinalis, 15 parts of saltcake, plate 45 parts of blue root, 30 parts of the coptis, 20 parts of bulbus fritillariae cirrhosae, 15 parts of the tuber of pinellia, 35 parts of cape jasmine, 25 parts of campanulaceae, 45 parts of Radix Glycyrrhizae, 20 parts of Charred Triplet, 40 parts of almond, 15 parts of loguat leaf, 20 parts of purple perilla, 20 parts of birthwort.

4. it is a kind of as described in claims 1 or 2 or 3 for prevent and treat poison invade lung kidney type porcine reproductive and respiratory syndrome The preparation method of Chinese medicine composition, which comprises the following steps: each Chinese medicine is weighed according to weight part ratio, by each Chinese medicine It is put into medicine decocting tank, the pure water or 70% ethyl alcohol that 10 times of amounts are added into tank impregnate 1h, decoct 30min, and filtering obtains for the first time Decoct filtrate；The pure water or 70% ethyl alcohol that 10 times of amounts are added into filter residue decoct 30 min, filter to obtain second of decoction filter Liquid；Merge filtrate twice, is concentrated under reduced pressure into medicinal extract, drying is sprayed into powder；Gained filter residue dries pulverizing, it is mixed with powder obtained by filtrate It closes uniformly, packing pack.