CN109717050B - Culture medium for cinnamomum camphora - Google Patents
Culture medium for cinnamomum camphora Download PDFInfo
- Publication number
- CN109717050B CN109717050B CN201910131898.9A CN201910131898A CN109717050B CN 109717050 B CN109717050 B CN 109717050B CN 201910131898 A CN201910131898 A CN 201910131898A CN 109717050 B CN109717050 B CN 109717050B
- Authority
- CN
- China
- Prior art keywords
- culture medium
- manure
- powder
- particles
- straw
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention provides a culture medium of camphor, belonging to the technical field of seedling cultivation, comprising (a) 10-25 wt% of decomposed fertilizer particles; (b) 20-25 wt% of straw particles; (c) 5-15 wt% of bio-peat; (d) 5-15 wt% of camphor leaf granules; and (e) 20-60 wt% of garden soil, wherein the preparation process of the decomposed fertilizer granules comprises the step of adding a degradation auxiliary agent containing myrobalic acid and canaglibose into farmyard livestock manure. The beneficial effects are that: the culture medium takes agricultural wastes as raw materials, the cost is low, the culture medium is environment-friendly, the culture survival rate of the cinnamomum camphora is obviously improved, the decomposition efficiency of decomposed manure in the medium can be improved by more than 50%, the time required for fully decomposing manure is greatly shortened, and the biological peat prepared by taking the cotton hull powder and the furfural residues as the raw materials can completely replace natural turf to play a role.
Description
Technical Field
The invention belongs to the technical field of seedling cultivation, and particularly relates to a culture medium for cinnamomum camphora.
Background
The Cinnamomum camphora is evergreen big tree of Lauraceae of Laurales, with height up to 30m, diameter up to 3m, and broad-egg-shaped crown; the tree crown is wide and the branches and leaves are dense, the vigor is strong, and the tree is a good greening tree, street tree and shade tree. Producing regions of south China and southwest provinces. Vietnam, Korea and Japan are distributed, and other countries often breed introduced cultures. Lignum Cinnamomi Camphorae contains Camphora and aromatic volatile oil (named ZHANG OIL). The plants have fragrance of Camphora, and can be extracted to obtain Camphora and oleum Camphora. The wood is hard and beautiful, and is suitable for manufacturing furniture and boxes. The camphor tree has resistance to harmful gases such as chlorine, sulfur dioxide, ozone, fluorine gas and the like, can repel mosquitoes and flies, can resist short-term flooding, and is a main raw material for producing camphor. The material is superior and is a good material for manufacturing furniture. The cinnamomum camphora breeding method mostly adopts seed breeding and cutting breeding, however, when the cinnamomum camphora is used for cultivation or urban greening, the structure of the culture medium cannot be matched with the growth requirement of the cinnamomum camphora, and the condition of poor growth, premature senility or death of the trees is easily caused, so how to provide the culture medium for the cinnamomum camphora has a profound and important significance for improving the management effect of the growth conditions of the cinnamomum camphora, improving the growth quality of the cinnamomum camphora and fully playing the service role of the urban ecosystem of the cinnamomum camphora.
The agricultural waste compost is treated and then used as a substrate to form a good utilization mode, at present, the agricultural waste compost is fully decomposed, and the piled compost is used as a fertilizer to provide better organic matters and a nitrogen source for soil. However, the time required for full decomposition is long, and the initial quality of different agricultural wastes is different, so that how to fully decompose in a short time is a difficult point of composting treatment. For example, the Chinese patent with the prior art application number of 201710966561.0 discloses a corncob fermentation substrate and a preparation method and application thereof, and a culture substrate with excellent performance is obtained by determining a fermentation end point through controlling the physicochemical properties such as water holding porosity, air permeability porosity, volume weight and the like in the corncob fermentation process. The culture medium has short fermentation time and good physical and chemical properties, and the substrate prepared by the method can increase the root system temperature of plants during winter planting and promote the growth of the plants. However, in the method, the period of stacking and fermenting the material II is as long as 30-40 d, and the period is long, so that the compost application is limited.
Disclosure of Invention
The invention aims to provide a culture medium of camphor trees, which takes agricultural wastes as raw materials, has low cost and is environment-friendly, and simultaneously, the culture survival rate of the camphor trees is obviously improved, the rotten efficiency of rotten fertilizers in the medium can be improved by more than 50 percent, the time required for fully rotting the manure is greatly shortened, and biological peat prepared by taking cotton hull powder and furfural residues as raw materials can completely replace natural turf to play a role.
The technical scheme adopted by the invention for realizing the purpose is as follows:
the invention provides a culture medium of cinnamomum camphora, which comprises:
(a) decomposing fertilizer particles;
(b) straw particles;
(c) bio-peat;
(d) cinnamomum camphora leaf granules; and
(e) and (6) garden soil.
The preparation process of the decomposed fertilizer granules comprises the step of adding a degradation auxiliary agent containing myrobalic acid and canaglibose into farmyard livestock manure.
The following in the culture medium of cinnamomum camphora may be present in the culture medium in the following weight percentages, based on the total amount of the culture medium:
(a) 10-25 wt% of decomposed fertilizer particles;
(b) 20-25 wt% of straw particles;
(c) 5-15 wt% of bio-peat;
(d)5 to 15 wt% of Cinnamomum camphora leaf granules and
(e) 20-60 wt% of garden soil; wherein the content of the first and second substances,
the particle size of the decomposed fertilizer particles is not more than 2 mm;
the particle size of the straw particles is not more than 3 mm;
the particle size of the camphor leaf granules is not more than 1 mm.
The culture medium of the cinnamomum camphora takes agricultural wastes as raw materials, so that the treatment cost of the wastes is reduced, the environment is also included, the full-effect utilization of the materials is realized, and the economic benefit is created; the content of anti-nutritional factors in decomposed manure particles in the culture medium is reduced, a certain amount of nitrogen, phosphorus and potassium are contained, the trace elements are rich, the culture medium is suitable for being used as a culture medium of camphor trees, the addition of the degradation auxiliary agent can improve the decomposition efficiency by more than 50%, the time required for fully decomposing manure is greatly shortened, and the production efficiency and the economic benefit are improved; the higher specific surface area of the straw particles is beneficial to enhancing the water retention capacity of the straw particles, is beneficial to disintegration of the straw particles and endows the substrate with excellent water regulation effect; the biological peat prepared from the cotton shell powder, the pond bottom mud and the furfural residues can completely replace the traditional non-renewable turf, well provide nutrition for camphor tree seedlings and ensure the culture survival rate of the camphor tree seedlings; compared with the traditional culture medium, the culture medium of the cinnamomum camphora can improve the transplanting survival rate of cinnamomum camphora seedlings by more than 30%, improve the growth quality of the cinnamomum camphora, and has profound and important significance in fully playing the service role of the urban ecosystem of the cinnamomum camphora.
Preferably, the preparation process of the decomposed fertilizer particles is as follows:
a) airing the farmyard livestock manure until the water content is 45-50 wt%, taking 300-450 parts by weight of the farmyard livestock manure, 60-80 parts by weight of vermiculite powder, 45-50 parts by weight of straw fragments and 15-20 parts by weight of edible fungus residues, composting and decomposing for 2-5 days to obtain manure I; after anaerobic fermentation and decomposition, insect eggs, grass seeds and the like in the manure of farmer livestock are killed, and the cellulose contained in the manure is partially hydrolyzed, so that the subsequent further fermentation is facilitated;
b) uniformly doping 3-4 parts by weight of zymophyte agent and 3-15 parts by weight of prodegradant into the manure I, fully and uniformly stirring, then composting, controlling the composting temperature to be 50-65 ℃, turning the compost every 1-2 d, fermenting for 5-10 d to obtain manure II, granulating the manure II until the grain size is not more than 2mm, and air-drying to obtain the fertilizer II; after the secondary fermentation and decomposition, insect eggs, grass seeds, harmful microorganisms and the like in the manure are completely killed, the content of anti-nutritional factors in the manure and the edible fungus residues is reduced, the degradation assistant can effectively improve the reduction efficiency of the fermentation microbial inoculum on substrates, the fermentation and decomposition are remarkably accelerated, the manure contains various nutrient elements such as humic acid, organic matters, mineral substances, saccharides and the like to supply nutrients, the recycling of the manure, straws and the edible fungus residues of farm animals can be effectively promoted, the manure contains a certain amount of nitrogen, phosphorus and potassium, and the trace elements are rich in content, so that the manure is suitable to be used as a culture medium of the cinnamomum camphora.
Further preferably, in the preparation process of the decomposed fertilizer particles, the fermentation inoculant is a mixture inoculant of bacillus subtilis, lactobacillus, bacillus mucilaginosus and aspergillus in a weight ratio of 1: 0.2-0.8: 0.1-0.3: 0.2-1; the fermentation inoculant uses a plurality of different strains for mixed fermentation, is favorable for exerting the advantages of the respective strains, is favorable for full synergy among the strains, has complementary advantages and greatly improves the decomposition efficiency.
Further preferably, the degradation auxiliary agent is an aqueous solution containing 55-75 mg/L of myrobalic acid and 13-15 mg/L of canaglibose; the tricarboxylic acid cycle is very important for the growth, the propagation and the metabolism of microorganisms, the microorganism is stressed after a trace amount of myrobalamin and the canaglibose in the degradation auxiliary agent enter the microorganism, the stress promotes the microorganism to strengthen the secretion of acetyl coenzyme A, the activity and the content of the acetyl coenzyme A in the microorganism are improved, and further the condensation reaction efficiency of the acetyl coenzyme A and the oxaloacetic acid is enhanced, so that the generation and the implementation of the tricarboxylic acid cycle in the microorganism are accelerated, the ATP output in unit time is finally improved, more energy is provided for the microorganism, the growth, the propagation and the metabolism of the microorganism are promoted, the degradation and the fermentation of the microorganism on a substrate are accelerated, the decomposition efficiency is improved, the decomposition efficiency can be improved by more than 50% by adding the degradation auxiliary agent, the time for fully decomposing manure is greatly shortened, and the production efficiency and the economic benefit are improved.
Preferably, the preparation process of the straw particles comprises the following steps: uniformly mixing 100 parts by weight of straw powder and 25-50 parts by weight of bran powder, adding concentrated sulfuric acid, soaking for 5-15 min, filtering, adding 5-10 parts by weight of distiller's grain dry powder into filter residues, adding water, mashing for 30-45 min, adding 5 wt% of cellulase as a substrate, fermenting, inactivating enzyme at high temperature, dehydrating, granulating until the particle size is not more than 3mm, and air-drying to obtain straw particles; the straw particles are loose, the content of sugar in the straw particles is high, the straw powder and the bran powder can be reduced after being soaked in concentrated sulfuric acid, cracks and/or holes are formed on the surface of the powder, the specific surface area of the powder is increased, the degradation of follow-up cellulase to powdery cellulose is facilitated, the fermentation efficiency is improved, meanwhile, the high specific surface area of the straw powder and the bran powder is beneficial to enhancing the water holding capacity of the straw powder and the bran powder, the disintegration of the straw powder and the bran powder is facilitated, and the excellent water regulating effect is given to the matrix.
Preferably, the preparation process of the bio-peat comprises the following steps:
a) crushing cotton hulls into cotton hull powder, uniformly mixing 100 parts by weight of cotton hull powder, 12-30 parts by weight of pond bottom mud and 8-12 parts by weight of compound fertilizer, adjusting the water content to 60-70 wt%, and uniformly stirring to obtain a premix; the pond bottom mud contains rich organic matters and mineral substances, and microorganisms in the pond bottom mud can perform degradation and decomposition effects on the premix;
b) performing closed anaerobic fermentation on the premix until the premix turns black, namely the fermentation end point, adding 5-30 parts by weight of furfural residues and 300-500 parts by weight of water, and boiling to obtain a final mixed material; after anaerobic fermentation, the microorganisms in the pond bottom mud thoroughly degrade the cotton hull powder, organic matters in the furfural residues can be decomposed to a greater degree at a high temperature, and the microorganisms are removed at the same time, so that the storage and transportation of the final biological peat are facilitated;
c) naturally drying the final mixed material to obtain a solid, and crushing the solid to 60-80 meshes to obtain the powder; the bio-peat is prepared from various components in a specific ratio, can completely replace turf as a camphor tree culture medium, well provides nutrition for camphor tree seedlings, ensures the culture survival rate of the camphor tree seedlings, and simultaneously replaces natural turf to play a role, thereby being beneficial to environmental protection and ecological balance.
The invention also provides a preparation method of the culture medium of cinnamomum camphora, which comprises the following steps:
(1) preparing decomposed fertilizer particles;
(2) preparing straw particles;
(3) preparing biological peat;
(4) according to the weight ratio, 10-25 wt% of decomposed fertilizer particles, 20-25 wt% of straw particles, 5-15 wt% of bio-peat, 5-15 wt% of camphor leaf granules and 20-60 wt% of garden soil are mixed to prepare the culture medium of the camphor.
The invention also relates to application of the culture medium of the cinnamomum camphora in cultivation of the cinnamomum camphora.
The invention has the beneficial effects that:
when the decomposed fertilizer particles are prepared, the micro myrobalan acid and the canaglibose in the degradation auxiliary agent are stressed on microorganisms after entering the microorganisms, the tricarboxylic acid cycle of the microorganisms is accelerated, the growth, the propagation and the metabolism of the microorganisms are enhanced, the degradation and the fermentation of the microorganisms on substrates are accelerated, the decomposing efficiency is improved, the decomposing efficiency can be improved by more than 50 percent by adding the degradation auxiliary agent, and the time for fully decomposing the manure is greatly shortened; after the straw powder and the bran powder are soaked in concentrated sulfuric acid, cracks and/or holes are formed on the surface of the powder, the specific surface area of the powder is increased, the subsequent degradation of powdery cellulose by cellulase is facilitated, the fermentation efficiency is improved, meanwhile, the higher specific surface areas of the straw powder and the bran powder are beneficial to enhancing the water binding capacity of the straw powder and the bran powder, the disintegration of the straw powder and the bran powder is facilitated, and the substrate is endowed with an excellent water regulating effect; the bio-peat is prepared from various components in a specific ratio, can completely replace turf as a camphor tree culture medium, well provides nutrition for camphor tree seedlings, ensures the culture survival rate of the camphor tree seedlings, and simultaneously replaces natural turf to play a role.
The culture medium for cinnamomum camphora provided by the invention adopts the technical scheme, overcomes the defects of the prior art, and is reasonable in design and convenient to operate.
Detailed Description
The present invention is further described in detail with reference to the following examples:
example 1:
the present embodiment provides a culture medium of cinnamomum camphora, wherein the following may be present in the culture medium in the following weight percentages based on the total amount of the culture medium:
(a)10 wt% of decomposed fertilizer particles;
(b)20 wt% straw particles;
(c)5 wt% of bio-peat;
(d)5 wt% of Cinnamomum camphora leaf granules and
(e)60 wt% garden soil; wherein the content of the first and second substances,
the particle size of the decomposed fertilizer particles is 2 mm;
the particle size of the straw particles is 3 mm;
the particle size of the camphor leaf granules is 1 mm.
The culture medium of the cinnamomum camphora takes agricultural wastes as raw materials, so that the treatment cost of the wastes is reduced, the environment is also included, the full-effect utilization of the materials is realized, and the economic benefit is created; the content of anti-nutritional factors in decomposed manure particles in the culture medium is reduced, a certain amount of nitrogen, phosphorus and potassium are contained, the trace elements are rich, the culture medium is suitable for being used as a culture medium of camphor trees, the addition of the degradation auxiliary agent can improve the decomposition efficiency by more than 50%, the time required for fully decomposing manure is greatly shortened, and the production efficiency and the economic benefit are improved; the higher specific surface area of the straw particles is beneficial to enhancing the water retention capacity of the straw particles, is beneficial to disintegration of the straw particles and endows the substrate with excellent water regulation effect; the biological peat prepared from the cotton shell powder, the pond bottom mud and the furfural residues can completely replace the traditional non-renewable turf, well provide nutrition for camphor tree seedlings and ensure the culture survival rate of the camphor tree seedlings; compared with the traditional culture medium, the culture medium of the cinnamomum camphora can improve the transplanting survival rate of cinnamomum camphora seedlings by more than 30%, improve the growth quality of the cinnamomum camphora, and has profound and important significance in fully playing the service role of the urban ecosystem of the cinnamomum camphora.
The preparation process of the decomposed fertilizer particles comprises the following steps:
a) airing the farmyard livestock manure until the water content is 45 wt%, and composting and decomposing 300 parts by weight of the farmyard livestock manure, 60 parts by weight of vermiculite powder, 45 parts by weight of straw fragments and 15 parts by weight of edible fungus residues for 2 days to obtain manure I; after anaerobic fermentation and decomposition, insect eggs, grass seeds and the like in the manure of farmer livestock are killed, and the cellulose contained in the manure is partially hydrolyzed, so that the subsequent further fermentation is facilitated;
b) uniformly doping 3 parts by weight of zymophyte agent and 3 parts by weight of prodegradant into the manure I, fully and uniformly stirring, then composting, controlling the composting temperature to be 50 ℃, turning over the compost once every day, fermenting for 5 days to obtain manure II, granulating the manure II to obtain the grain size of 2mm, and air-drying to obtain the fertilizer II; after the secondary fermentation and decomposition, insect eggs, grass seeds, harmful microorganisms and the like in the manure are completely killed, the content of anti-nutritional factors in the manure and the edible fungus residues is reduced, the degradation assistant can effectively improve the reduction efficiency of the fermentation microbial inoculum on substrates, the fermentation and decomposition are remarkably accelerated, the manure contains various nutrient elements such as humic acid, organic matters, mineral substances, saccharides and the like to supply nutrients, the recycling of the manure, straws and the edible fungus residues of farm animals can be effectively promoted, the manure contains a certain amount of nitrogen, phosphorus and potassium, and the trace elements are rich in content, so that the manure is suitable to be used as a culture medium of the cinnamomum camphora.
In the preparation process of the decomposed fertilizer particles, the fermentation microbial inoculum is a mixture microbial inoculum of bacillus subtilis, lactobacillus, bacillus mucilaginosus and aspergillus in a weight ratio of 1:0.2:0.1: 0.2; the fermentation inoculant uses a plurality of different strains for mixed fermentation, is favorable for exerting the advantages of the respective strains, is favorable for full synergy among the strains, has complementary advantages and greatly improves the decomposition efficiency.
The degradation auxiliary agent is an aqueous solution containing 55mg/L of myrobalam acid and 13mg/L of canaglibose; the tricarboxylic acid cycle is very important for the growth, the propagation and the metabolism of microorganisms, the microorganism is stressed after a trace amount of myrobalamin and the canaglibose in the degradation auxiliary agent enter the microorganism, the stress promotes the microorganism to strengthen the secretion of acetyl coenzyme A, the activity and the content of the acetyl coenzyme A in the microorganism are improved, and further the condensation reaction efficiency of the acetyl coenzyme A and the oxaloacetic acid is enhanced, so that the generation and the implementation of the tricarboxylic acid cycle in the microorganism are accelerated, the ATP output in unit time is finally improved, more energy is provided for the microorganism, the growth, the propagation and the metabolism of the microorganism are promoted, the degradation and the fermentation of the microorganism on a substrate are accelerated, the decomposition efficiency is improved, the decomposition efficiency can be improved by more than 50% by adding the degradation auxiliary agent, the time for fully decomposing manure is greatly shortened, and the production efficiency and the economic benefit are improved.
The preparation process of the straw particles comprises the following steps: uniformly mixing 100 parts by weight of straw powder and 25 parts by weight of bran powder, adding concentrated sulfuric acid, soaking for 5min, filtering, adding 5 parts by weight of distiller's grains dry powder into filter residues, adding water, mashing for 30min, adding 5 wt% of cellulase as a substrate, performing high-temperature enzyme inactivation after fermentation, dehydrating, granulating, and drying to obtain straw particles, wherein the particle size of the particles is not more than 3 mm; the straw particles are loose, the content of sugar in the straw particles is high, the straw powder and the bran powder can be reduced after being soaked in concentrated sulfuric acid, cracks and/or holes are formed on the surface of the powder, the specific surface area of the powder is increased, the degradation of follow-up cellulase to powdery cellulose is facilitated, the fermentation efficiency is improved, meanwhile, the high specific surface area of the straw powder and the bran powder is beneficial to enhancing the water holding capacity of the straw powder and the bran powder, the disintegration of the straw powder and the bran powder is facilitated, and the excellent water regulating effect is given to the matrix.
The preparation process of the bio-peat comprises the following steps:
a) crushing cotton hulls into cotton hull powder, uniformly mixing 100 parts by weight of cotton hull powder, 12 parts by weight of pond bottom mud and 8 parts by weight of compound fertilizer, adjusting the water content to be 60 wt%, and uniformly stirring to obtain a premix; the pond bottom mud contains rich organic matters and mineral substances, and microorganisms in the pond bottom mud can perform degradation and decomposition effects on the premix;
b) performing closed anaerobic fermentation on the premix until the premix turns black, namely the fermentation end point, adding 5 parts by weight of furfural residues and 300 parts by weight of water, and boiling to obtain a final mixed material; after anaerobic fermentation, the microorganisms in the pond bottom mud thoroughly degrade the cotton hull powder, organic matters in the furfural residues can be decomposed to a greater degree at a high temperature, and the microorganisms are removed at the same time, so that the storage and transportation of the final biological peat are facilitated;
c) naturally drying the final mixed material to obtain a solid, and crushing the solid to 60 meshes to obtain the powder; the bio-peat is prepared from various components in a specific ratio, can completely replace turf as a camphor tree culture medium, well provides nutrition for camphor tree seedlings, ensures the culture survival rate of the camphor tree seedlings, and simultaneously replaces natural turf to play a role, thereby being beneficial to environmental protection and ecological balance.
The embodiment also provides a preparation method of the culture medium of cinnamomum camphora, which comprises the following steps:
(1) preparing decomposed fertilizer particles;
(2) preparing straw particles;
(3) preparing biological peat;
(4) according to the weight ratio, 10 wt% of decomposed fertilizer particles, 20 wt% of straw particles, 5 wt% of bio-peat, 5 wt% of camphor leaf granules and 60 wt% of garden soil are mixed to prepare the culture medium of the camphor.
Example 2:
a culture medium for Cinnamomum camphora, comprising:
(a) decomposing fertilizer particles;
(b) straw particles;
(c) bio-peat;
(d) cinnamomum camphora leaf granules; and
(e) and (6) garden soil.
The following in the culture medium of cinnamomum camphora may be present in the culture medium in the following weight percentages, based on the total amount of the culture medium:
(a)25 wt% decomposed fertilizer particles;
(b)25 wt% straw particles;
(c)15 wt% bio-peat;
(d)15 wt% of Cinnamomum camphora leaf granules and
(e)20 wt% garden soil; wherein the content of the first and second substances,
the particle size of the decomposed fertilizer particles is 0.5 mm;
the particle size of the straw particles is 0.5 mm;
the particle size of the camphor leaf granules is 0.5 mm.
The invention also provides a preparation method of the culture medium of cinnamomum camphora, which comprises the following steps:
(1) preparing decomposed fertilizer particles;
(2) preparing straw particles;
(3) preparing biological peat;
(4) according to the weight ratio, 25 wt% of decomposed fertilizer particles, 25 wt% of straw particles, 15 wt% of bio-peat, 15 wt% of camphor leaf granules and 20 wt% of garden soil are mixed to prepare the culture medium of the camphor.
The culture medium of the cinnamomum camphora takes agricultural wastes as raw materials, so that the treatment cost of the wastes is reduced, the environment is also included, the full-effect utilization of the materials is realized, and the economic benefit is created; the content of anti-nutritional factors in decomposed manure particles in the culture medium is reduced, a certain amount of nitrogen, phosphorus and potassium are contained, the trace elements are rich, the culture medium is suitable for being used as a culture medium of camphor trees, the addition of the degradation auxiliary agent can improve the decomposition efficiency by more than 50%, the time required for fully decomposing manure is greatly shortened, and the production efficiency and the economic benefit are improved; the higher specific surface area of the straw particles is beneficial to enhancing the water retention capacity of the straw particles, is beneficial to disintegration of the straw particles and endows the substrate with excellent water regulation effect; the biological peat prepared from the cotton shell powder, the pond bottom mud and the furfural residues can completely replace the traditional non-renewable turf, well provide nutrition for camphor tree seedlings and ensure the culture survival rate of the camphor tree seedlings; compared with the traditional culture medium, the culture medium of the cinnamomum camphora can improve the transplanting survival rate of cinnamomum camphora seedlings by more than 30%, improve the growth quality of the cinnamomum camphora, and has profound and important significance in fully playing the service role of the urban ecosystem of the cinnamomum camphora.
The preparation process of the decomposed fertilizer particles comprises the following steps:
a) airing the farmyard livestock manure until the water content is 50 wt%, taking 450 parts by weight of the farmyard livestock manure, 80 parts by weight of vermiculite powder, 50 parts by weight of straw fragments and 20 parts by weight of edible fungus residues, composting and decomposing for 5 days to obtain manure I; after anaerobic fermentation and decomposition, insect eggs, grass seeds and the like in the manure of farmer livestock are killed, and the cellulose contained in the manure is partially hydrolyzed, so that the subsequent further fermentation is facilitated;
b) uniformly doping 4 parts by weight of zymophyte agent and 15 parts by weight of prodegradant into the manure I, fully and uniformly stirring, then composting, controlling the composting temperature to 65 ℃, turning over every 2 days, fermenting for 10 days to obtain manure II, granulating the manure II to obtain the grain size of 0.5mm, and air-drying to obtain the fertilizer II; after the secondary fermentation and decomposition, insect eggs, grass seeds, harmful microorganisms and the like in the manure are completely killed, the content of anti-nutritional factors in the manure and the edible fungus residues is reduced, the degradation assistant can effectively improve the reduction efficiency of the fermentation microbial inoculum on substrates, the fermentation and decomposition are remarkably accelerated, the manure contains various nutrient elements such as humic acid, organic matters, mineral substances, saccharides and the like to supply nutrients, the recycling of the manure, straws and the edible fungus residues of farm animals can be effectively promoted, the manure contains a certain amount of nitrogen, phosphorus and potassium, and the trace elements are rich in content, so that the manure is suitable to be used as a culture medium of the cinnamomum camphora.
In the preparation process of the decomposed fertilizer particles, the fermentation microbial inoculum is a mixture microbial inoculum of bacillus subtilis, lactobacillus, bacillus mucilaginosus and aspergillus in a weight ratio of 1:0.8:0.3: 1; the fermentation inoculant uses a plurality of different strains for mixed fermentation, is favorable for exerting the advantages of the respective strains, is favorable for full synergy among the strains, has complementary advantages and greatly improves the decomposition efficiency.
In the preparation process of the decomposed fertilizer particles, the degradation auxiliary agent is an aqueous solution containing 75mg/L of myrobalamin and 15mg/L of canaglibose; the tricarboxylic acid cycle is very important for the growth, the propagation and the metabolism of microorganisms, the microorganism is stressed after a trace amount of myrobalamin and the canaglibose in the degradation auxiliary agent enter the microorganism, the stress promotes the microorganism to strengthen the secretion of acetyl coenzyme A, the activity and the content of the acetyl coenzyme A in the microorganism are improved, and further the condensation reaction efficiency of the acetyl coenzyme A and the oxaloacetic acid is enhanced, so that the generation and the implementation of the tricarboxylic acid cycle in the microorganism are accelerated, the ATP output in unit time is finally improved, more energy is provided for the microorganism, the growth, the propagation and the metabolism of the microorganism are promoted, the degradation and the fermentation of the microorganism on a substrate are accelerated, the decomposition efficiency is improved, the decomposition efficiency can be improved by more than 50% by adding the degradation auxiliary agent, the time for fully decomposing manure is greatly shortened, and the production efficiency and the economic benefit are improved.
The preparation process of the straw particles comprises the following steps: uniformly mixing 100 parts by weight of straw powder and 50 parts by weight of bran powder, adding concentrated sulfuric acid, soaking for 15min, filtering, adding 10 parts by weight of distiller's grains dry powder into filter residues, adding water, mashing for 45min, adding 5 wt% of cellulase as a substrate, performing high-temperature enzyme inactivation after fermentation, dehydrating, granulating until the particle size is not more than 3mm, and air-drying to obtain straw particles; the straw particles are loose, the content of sugar in the straw particles is high, the straw powder and the bran powder can be reduced after being soaked in concentrated sulfuric acid, cracks and/or holes are formed on the surface of the powder, the specific surface area of the powder is increased, the degradation of follow-up cellulase to powdery cellulose is facilitated, the fermentation efficiency is improved, meanwhile, the high specific surface area of the straw powder and the bran powder is beneficial to enhancing the water holding capacity of the straw powder and the bran powder, the disintegration of the straw powder and the bran powder is facilitated, and the excellent water regulating effect is given to the matrix.
The preparation process of the bio-peat comprises the following steps:
a) crushing cotton hulls into cotton hull powder, uniformly mixing 100 parts by weight of cotton hull powder, 30 parts by weight of pond bottom mud and 12 parts by weight of compound fertilizer, adjusting the water content to 70 wt%, and uniformly stirring to obtain a premix; the pond bottom mud contains rich organic matters and mineral substances, and microorganisms in the pond bottom mud can perform degradation and decomposition effects on the premix;
b) performing closed anaerobic fermentation on the premix until the premix turns black, namely the fermentation end point, adding 5-30 parts by weight of furfural residues and 300-500 parts by weight of water, and boiling to obtain a final mixed material; after anaerobic fermentation, the microorganisms in the pond bottom mud thoroughly degrade the cotton hull powder, organic matters in the furfural residues can be decomposed to a greater degree at a high temperature, and the microorganisms are removed at the same time, so that the storage and transportation of the final biological peat are facilitated;
c) naturally drying the final mixed material to obtain a solid, and crushing the solid to 80 meshes to obtain the powder; the bio-peat is prepared from various components in a specific ratio, can completely replace turf as a camphor tree culture medium, well provides nutrition for camphor tree seedlings, ensures the culture survival rate of the camphor tree seedlings, and simultaneously replaces natural turf to play a role, thereby being beneficial to environmental protection and ecological balance.
Example 3:
the embodiment provides a culture medium of cinnamomum camphora, comprising:
(a) decomposing fertilizer particles;
(b) straw particles;
(c) bio-peat;
(d) cinnamomum camphora leaf granules; and
(e) and (6) garden soil.
The following in the culture medium of cinnamomum camphora may be present in the culture medium in the following weight percentages, based on the total amount of the culture medium:
(a)15 wt% of decomposed fertilizer particles;
(b)25 wt% straw particles;
(c)10 wt% bio-peat;
(d)10 wt% of Cinnamomum camphora leaf granules and
(e)40 wt% garden soil; wherein the content of the first and second substances,
the particle size of the decomposed fertilizer particles is 1 mm;
the particle size of the straw particles is 2 mm;
the particle size of the camphor leaf granules is 0.8 mm.
The embodiment also provides a preparation method of the culture medium based on the cinnamomum camphora, which comprises the following steps:
(1) preparing decomposed fertilizer particles;
(2) preparing straw particles;
(3) preparing biological peat;
(4) mixing 15 wt% of decomposed fertilizer particles, 25 wt% of straw particles, 10 wt% of bio-peat, 10 wt% of camphor leaf granules and 40 wt% of garden soil according to the weight ratio to prepare the culture medium of the camphor.
The culture medium of the cinnamomum camphora takes agricultural wastes as raw materials, so that the treatment cost of the wastes is reduced, the environment is also included, the full-effect utilization of the materials is realized, and the economic benefit is created; the content of anti-nutritional factors in decomposed manure particles in the culture medium is reduced, a certain amount of nitrogen, phosphorus and potassium are contained, the trace elements are rich, the culture medium is suitable for being used as a culture medium of camphor trees, the addition of the degradation auxiliary agent can improve the decomposition efficiency by more than 50%, the time required for fully decomposing manure is greatly shortened, and the production efficiency and the economic benefit are improved; the higher specific surface area of the straw particles is beneficial to enhancing the water retention capacity of the straw particles, is beneficial to disintegration of the straw particles and endows the substrate with excellent water regulation effect; the biological peat prepared from the cotton shell powder, the pond bottom mud and the furfural residues can completely replace the traditional non-renewable turf, well provide nutrition for camphor tree seedlings and ensure the culture survival rate of the camphor tree seedlings; compared with the traditional culture medium, the culture medium of the cinnamomum camphora can improve the transplanting survival rate of cinnamomum camphora seedlings by more than 30%, improve the growth quality of the cinnamomum camphora, and has profound and important significance in fully playing the service role of the urban ecosystem of the cinnamomum camphora.
In the culture medium of the cinnamomum camphora, the preparation process of the decomposed fertilizer particles is as follows:
a) airing the farmyard livestock manure until the water content is 45 wt%, and composting and decomposing 400 parts by weight of the farmyard livestock manure, 75 parts by weight of vermiculite powder, 45 parts by weight of straw fragments and 18 parts by weight of edible fungus residues for 4 days to obtain manure I; after anaerobic fermentation and decomposition, insect eggs, grass seeds and the like in the manure of farmer livestock are killed, and the cellulose contained in the manure is partially hydrolyzed, so that the subsequent further fermentation is facilitated;
b) uniformly doping 4 parts by weight of zymophyte agent and 10 parts by weight of prodegradant into the manure I, fully and uniformly stirring, then composting, controlling the composting temperature to be 60 ℃, turning over every 2d, fermenting for 8d to obtain manure II, granulating the manure II with the grain diameter of 1mm, and air-drying to obtain the fertilizer II; after the secondary fermentation and decomposition, insect eggs, grass seeds, harmful microorganisms and the like in the manure are completely killed, the content of anti-nutritional factors in the manure and the edible fungus residues is reduced, the degradation assistant can effectively improve the reduction efficiency of the fermentation microbial inoculum on substrates, the fermentation and decomposition are remarkably accelerated, the manure contains various nutrient elements such as humic acid, organic matters, mineral substances, saccharides and the like to supply nutrients, the recycling of the manure, straws and the edible fungus residues of farm animals can be effectively promoted, the manure contains a certain amount of nitrogen, phosphorus and potassium, and the trace elements are rich in content, so that the manure is suitable to be used as a culture medium of the cinnamomum camphora.
In the preparation process of the decomposed fertilizer particles, the fermentation microbial inoculum is a mixture microbial inoculum of bacillus subtilis, lactobacillus, bacillus mucilaginosus and aspergillus in a weight ratio of 1:0.5:0.2: 0.5; the fermentation inoculant uses a plurality of different strains for mixed fermentation, is favorable for exerting the advantages of the respective strains, is favorable for full synergy among the strains, has complementary advantages and greatly improves the decomposition efficiency.
The degradation auxiliary agent is an aqueous solution containing 60mg/L of myrobalamin and 14mg/L of canaglibose; the tricarboxylic acid cycle is very important for the growth, the propagation and the metabolism of microorganisms, the microorganism is stressed after a trace amount of myrobalamin and the canaglibose in the degradation auxiliary agent enter the microorganism, the stress promotes the microorganism to strengthen the secretion of acetyl coenzyme A, the activity and the content of the acetyl coenzyme A in the microorganism are improved, and further the condensation reaction efficiency of the acetyl coenzyme A and the oxaloacetic acid is enhanced, so that the generation and the implementation of the tricarboxylic acid cycle in the microorganism are accelerated, the ATP output in unit time is finally improved, more energy is provided for the microorganism, the growth, the propagation and the metabolism of the microorganism are promoted, the degradation and the fermentation of the microorganism on a substrate are accelerated, the decomposition efficiency is improved, the decomposition efficiency can be improved by more than 50% by adding the degradation auxiliary agent, the time for fully decomposing manure is greatly shortened, and the production efficiency and the economic benefit are improved.
In the culture medium of the cinnamomum camphora, the preparation process of the straw particles is as follows: uniformly mixing 100 parts by weight of straw powder and 40 parts by weight of bran powder, adding concentrated sulfuric acid, soaking for 10min, filtering, adding 8 parts by weight of distiller's grains dry powder into filter residues, adding water, mashing for 35min, adding 5 wt% of cellulase as a substrate, performing high-temperature enzyme inactivation after fermentation, dehydrating, granulating, and drying to obtain straw particles, wherein the particle size of the particles is 2 mm; the straw particles are loose, the content of sugar in the straw particles is high, the straw powder and the bran powder can be reduced after being soaked in concentrated sulfuric acid, cracks and/or holes are formed on the surface of the powder, the specific surface area of the powder is increased, the degradation of follow-up cellulase to powdery cellulose is facilitated, the fermentation efficiency is improved, meanwhile, the high specific surface area of the straw powder and the bran powder is beneficial to enhancing the water holding capacity of the straw powder and the bran powder, the disintegration of the straw powder and the bran powder is facilitated, and the excellent water regulating effect is given to the matrix.
In the culture medium of the cinnamomum camphora, the preparation process of the biological peat comprises the following steps:
a) crushing cotton hulls into cotton hull powder, uniformly mixing 100 parts by weight of cotton hull powder, 25 parts by weight of pond bottom mud and 10 parts by weight of compound fertilizer, adjusting the water content to 65 wt%, and uniformly stirring to obtain a premix; the pond bottom mud contains rich organic matters and mineral substances, and microorganisms in the pond bottom mud can perform degradation and decomposition effects on the premix;
b) performing closed anaerobic fermentation on the premix until the premix turns black, namely the fermentation end point, adding 25 parts by weight of furfural residues and 400 parts by weight of water, and boiling to obtain a final mixed material; after anaerobic fermentation, the microorganisms in the pond bottom mud thoroughly degrade the cotton hull powder, organic matters in the furfural residues can be decomposed to a greater degree at a high temperature, and the microorganisms are removed at the same time, so that the storage and transportation of the final biological peat are facilitated;
c) naturally drying the final mixed material to obtain a solid, and crushing the solid to 60 meshes to obtain the powder; the bio-peat is prepared from various components in a specific ratio, can completely replace turf as a camphor tree culture medium, well provides nutrition for camphor tree seedlings, ensures the culture survival rate of the camphor tree seedlings, and simultaneously replaces natural turf to play a role, thereby being beneficial to environmental protection and ecological balance.
The embodiment also relates to the application of the culture medium of the cinnamomum camphora in the cultivation of the cinnamomum camphora.
Example 4:
example 4 is essentially the same as example 3, except that the bio-peat of example 4 is commercially available conventional peat.
Example 5:
example 5 is essentially the same as example 3, except that the prodegradant of example 5 is an aqueous solution containing 60mg/L myrobalic acid.
Example 6:
example 6 is essentially the same as example 3, except that the prodegradant of example 6 is an aqueous solution containing 14mg/L of canaglibose.
Example 7:
example 7 is essentially the same as example 3, except that the degradation aid of example 7 is water.
Example 8:
example 8 is substantially the same as example 3 except that the mixture of 100 parts by weight of straw powder and 40 parts by weight of bran powder in example 8 was soaked in clean water for 10min and then filtered.
Test example:
transplanting the seedlings of the cinnamomum camphora of the same batch into the culture medium in the embodiments 1-8, respectively, and counting the survival rate, the diameter growth rate of the seedlings and the height growth rate of the seedlings of the cinnamomum camphora of the embodiments 1-8 after 2 weeks of transplantation, wherein the counting results are shown in table 1.
TABLE 1 statistics of growth parameters of Cinnamomum camphora seedlings in examples 1-8
| Example one another | Survival rate (%) | Diameter growth Rate (%) | Height growth Rate (%) |
| Example 1 | 100.0％ | 5.2％ | 10.6％ |
| Example 2 | 100.0％ | 5.5％ | 10.5％ |
| Example 3 | 100.0％ | 5.8％ | 11.2％ |
| Example 4 | 100.0％ | 4.8％ | 10.0％ |
| Example 5 | 98.0％ | 4.2％ | 8.5％ |
| Example 6 | 97.0％ | 4.3％ | 8.4％ |
| Example 7 | 94.0％ | 3.8％ | 7.2％ |
| Example 8 | 96.0％ | 4.2％ | 8.3％ |
As can be seen from table 1, in the preferred embodiments 1 to 3 of the present invention, the transplanting survival rate of the cinnamomum camphora seedlings reaches 100%, and the thickness and height of the plants are significantly improved, and compared with the commercially available peat in embodiment 4, the peat in the present invention can completely replace the commercially available peat, and even has a better effect on the cinnamomum camphora seedlings, so the culture medium in the present invention is suitable for cultivation of cinnamomum camphora.
Conventional techniques in the above embodiments are known to those skilled in the art, and therefore, will not be described in detail herein.
The above embodiments are merely illustrative, and not restrictive, and those skilled in the art can make various changes and modifications without departing from the spirit and scope of the invention. Therefore, all equivalent technical solutions also belong to the scope of the present invention, and the protection scope of the present invention should be defined by the claims.
Claims (5)
1. The culture medium of cinnamomum camphora is characterized by comprising the following components in percentage by weight:
(a) 10-25 wt% of decomposed fertilizer particles;
(b) 20-25 wt% of straw particles;
(c) 5-15 wt% of bio-peat;
(d) 5-15 wt% of camphor leaf granules; and
(e) 20-60 wt% of garden soil; wherein the content of the first and second substances,
the preparation process of the decomposed fertilizer particles comprises the following steps:
a) composting and decomposing farmyard livestock manure, vermiculite powder, straw fragments and edible fungus residues to obtain manure I;
b) adding a fermentation microbial inoculum and a degradation auxiliary agent into the manure I, uniformly stirring, composting and fermenting to obtain manure II, and air-drying to obtain the fertilizer II;
the fermentation microbial inoculum is a mixture microbial inoculum of bacillus subtilis, lactobacillus, bacillus mucilaginosus and aspergillus in a weight ratio of 1: 0.2-0.8: 0.1-0.3: 0.2-1;
the degradation auxiliary agent is an aqueous solution containing 55-75 mg/L of myrobalam acid and 13-15 mg/L of canaglibose;
the preparation process of the straw particles comprises the following steps: mixing the straw powder and the bran powder, adding concentrated sulfuric acid, soaking for 5-15 min, filtering, adding the dry powder of the vinasse into filter residues, adding water, pulping for 30-45 min, adding cellulase, fermenting, inactivating enzyme at high temperature, and air-drying to obtain the feed.
2. The culture substrate according to claim 1, wherein:
the particle size of the decomposed fertilizer particles is not more than 2 mm; and/or
The particle size of the straw particles is not more than 3 mm; and/or
The particle size of the camphor leaf granules is not more than 1 mm.
3. The culture substrate according to claim 1, wherein: the preparation process of the bio-peat comprises the following steps:
a) uniformly mixing the cotton shell powder, the pond bottom mud and the compound fertilizer, adding water, and uniformly stirring to obtain a premix;
b) carrying out closed anaerobic fermentation on the premix until the premix turns black, adding furfural residues and water, and boiling to obtain a final mixed material;
c) and naturally drying the final mixed material to obtain a solid, and crushing the solid to 60-80 meshes to obtain the powder.
4. The method for preparing a culture medium for Cinnamomum camphora according to any one of claims 1 to 3, comprising:
(1) preparing decomposed fertilizer particles;
(2) preparing straw particles;
(3) preparing biological peat;
(4) mixing the decomposed fertilizer particles, the straw particles, the biological peat, the camphor leaf granules and garden soil to prepare the culture medium of the camphor.
5. The use of the culture medium of cinnamomum camphora according to any one of claims 1 to 3 in the cultivation of cinnamomum camphora.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910131898.9A CN109717050B (en) | 2019-02-22 | 2019-02-22 | Culture medium for cinnamomum camphora |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910131898.9A CN109717050B (en) | 2019-02-22 | 2019-02-22 | Culture medium for cinnamomum camphora |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109717050A CN109717050A (en) | 2019-05-07 |
| CN109717050B true CN109717050B (en) | 2021-04-06 |
Family
ID=66301543
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910131898.9A Active CN109717050B (en) | 2019-02-22 | 2019-02-22 | Culture medium for cinnamomum camphora |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109717050B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111943774B (en) * | 2020-07-14 | 2023-03-21 | 湖北绿道农业发展有限公司 | Functional biological organic fertilizer and preparation method thereof |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101050425A (en) * | 2006-04-05 | 2007-10-10 | 萧松溪 | Trichodermin capable of decomposing and fermenting organic composting material in animality |
| CN104761390A (en) * | 2015-04-20 | 2015-07-08 | 江苏里下河地区农业科学研究所 | Special matrix for cultivating camphor tree and preparation method of special matrix |
| CN105130582A (en) * | 2015-07-31 | 2015-12-09 | 成都圣灵生物科技有限公司 | Preparation method of black fungus culture matrix |
| CN105684851A (en) * | 2016-02-18 | 2016-06-22 | 黑龙江稻乐农业科技有限公司 | Soilless rice nursery straw substrate and preparation method thereof |
| CN106613817A (en) * | 2016-10-20 | 2017-05-10 | 何荣福 | Camphor tree cuttage substrate |
| CN106797822A (en) * | 2017-02-07 | 2017-06-06 | 上海市绿化管理指导站 | Plant matrix and cinnamomum camphora tree plant system for cinnamomum camphora tree |
| CN107056351A (en) * | 2017-04-28 | 2017-08-18 | 重庆理工大学 | A kind of fowl and animal excrement continuously ferments the method for compost |
| CN107879787A (en) * | 2017-11-27 | 2018-04-06 | 新昌县洋花园果木专业合作社 | A kind of Landscape nursery-stock growing matrix and preparation method thereof |
| CN109169162A (en) * | 2018-09-14 | 2019-01-11 | 金塔县金畜源牧业有限公司 | A kind of organic composite substratd and preparation method thereof |
| CN109258331A (en) * | 2018-10-08 | 2019-01-25 | 石洪广 | The cultural method of vegetables |
-
2019
- 2019-02-22 CN CN201910131898.9A patent/CN109717050B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101050425A (en) * | 2006-04-05 | 2007-10-10 | 萧松溪 | Trichodermin capable of decomposing and fermenting organic composting material in animality |
| CN104761390A (en) * | 2015-04-20 | 2015-07-08 | 江苏里下河地区农业科学研究所 | Special matrix for cultivating camphor tree and preparation method of special matrix |
| CN105130582A (en) * | 2015-07-31 | 2015-12-09 | 成都圣灵生物科技有限公司 | Preparation method of black fungus culture matrix |
| CN105684851A (en) * | 2016-02-18 | 2016-06-22 | 黑龙江稻乐农业科技有限公司 | Soilless rice nursery straw substrate and preparation method thereof |
| CN106613817A (en) * | 2016-10-20 | 2017-05-10 | 何荣福 | Camphor tree cuttage substrate |
| CN106797822A (en) * | 2017-02-07 | 2017-06-06 | 上海市绿化管理指导站 | Plant matrix and cinnamomum camphora tree plant system for cinnamomum camphora tree |
| CN107056351A (en) * | 2017-04-28 | 2017-08-18 | 重庆理工大学 | A kind of fowl and animal excrement continuously ferments the method for compost |
| CN107879787A (en) * | 2017-11-27 | 2018-04-06 | 新昌县洋花园果木专业合作社 | A kind of Landscape nursery-stock growing matrix and preparation method thereof |
| CN109169162A (en) * | 2018-09-14 | 2019-01-11 | 金塔县金畜源牧业有限公司 | A kind of organic composite substratd and preparation method thereof |
| CN109258331A (en) * | 2018-10-08 | 2019-01-25 | 石洪广 | The cultural method of vegetables |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109717050A (en) | 2019-05-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102617239B (en) | Compound type plant cultivation matrix and preparation method thereof | |
| CN102303991B (en) | Plant cultivation mixed medium and preparation method thereof | |
| CN102924185B (en) | Chicken manure fermented fertilizer | |
| CN104987269A (en) | Biological organic fertilizer special for apples and preparation method thereof | |
| CN101811903A (en) | Novel organic substrate and preparation method thereof | |
| CN102633544B (en) | Method for taking farmyard manure as raw material to produce tobacco biofertilizer | |
| CN103086755A (en) | Melon/vegetable seedling raising organic compound substrate prepared from vinasse and production method thereof | |
| CN105272421B (en) | Agricultural culture medium and production method and application thereof | |
| CN106187338B (en) | Soilless plant culture substrate and preparation method thereof | |
| CN105948846A (en) | Organic fertilizer special for potted flowers | |
| CN102627481A (en) | Preparation method of plant cultivation substrate and cultivation substrate prepared by such method | |
| CN108299120A (en) | A kind of preparation method of organic fertilizer | |
| CN105503333A (en) | Seedling raising medium special for organic rice | |
| CN103771924B (en) | A kind of flower planting fertilizer special for organic and preparation method thereof | |
| CN110972891B (en) | Ecological composite culture medium taking garden waste as main raw material and application thereof | |
| CN111492934A (en) | Novel environment-friendly tobacco floating seedling substrate and preparation method and application thereof | |
| CN111972254A (en) | Method for producing seedling raising substrate by using vegetable straw compost | |
| CN111197007A (en) | Production method of high-efficiency bacterial bricks for composting | |
| CN108617465B (en) | Ecological substrate for vegetation restoration and preparation method thereof | |
| CN103951479B (en) | Fertilizer and preparation method thereof | |
| CN112166731B (en) | Method for improving physical and chemical properties of soil | |
| CN106518536B (en) | Production method of special fertilizer for mulberry | |
| CN109717050B (en) | Culture medium for cinnamomum camphora | |
| CN110015936B (en) | Functional organic fertilizer for improving saline-alkali soil and preparation method thereof | |
| CN111587762A (en) | Environment-friendly vegetable seedling culture substrate and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |