CN109673671A - A kind of microorganism seed inorganic agent and preparation method for preventing and treating potato verticillium wilt - Google Patents
A kind of microorganism seed inorganic agent and preparation method for preventing and treating potato verticillium wilt Download PDFInfo
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- CN109673671A CN109673671A CN201910061211.9A CN201910061211A CN109673671A CN 109673671 A CN109673671 A CN 109673671A CN 201910061211 A CN201910061211 A CN 201910061211A CN 109673671 A CN109673671 A CN 109673671A
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- verticillium wilt
- prevention
- inorganic agent
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- potato
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
Abstract
The present invention relates to a kind of microorganism seed inorganic agents and preparation method for preventing and treating potato verticillium wilt, the active constituent of the microorganism seed inorganic agent of the prevention and treatment potato verticillium wilt is bacillus subtilis NCD-2 and bacillus amyloliquefaciens Ba-2 and its metabolite, additionally contain dispersing agent, wetting agent and filler, the component is subjected to appropriate weight proportion, the seed treatment with prevention and treatment verticillium wilt is finally prepared.Seed treatment of the present invention can colonize rapidly plant rhizosphere, the effects of by infecting site, secretion Substance with pathogen competition and plant nutrient being promoted to absorb, farthest plays Biocontrol microorganism induction disease resistance of plant and the persistently ability of control disease.Seed treatment of the present invention can reduce the access times and dosage of chemical bactericide, reduce cost；Delay the generation of drug resistance, nontoxic, non agricultural chemical residuum；Ecological Distribution of Soil Microorganisms fauna is adjusted, promotes plant growth, and there is production-increasing function.
Description
Technical field
The invention belongs to technical field of microbe application, and in particular to a kind of microorganism seed for preventing and treating potato verticillium wilt
Inorganic agent and preparation method.
Background technique
Potato is the important cereal crops in China, is had great importance to Ensuring Food Safety.China is by Ma Ling
Potato is classified as the fourth-largest staple food grain after rice, wheat, corn, and wide adaptability, yielding ability be good, full of nutrition, economic benefit
Height has extensive plantation in each ecological zone in China.In recent years, it is aggravated year by year since potato continuous cropping causes soil-borne disease to have
Trend, directly affect potato yield and quality, bring biggish economic loss to Potato Industry.
Potato verticillium wilt (Verticillium wilt of potato) is also known as early dead disease or precocious disease, gradually at
For one of the Major Diseases in potato production, it is that simultaneous kind of typical soil biography passes vascular bundle diseases, causes potato early dead, less serious case
Underproduction 20%-30%, severe one are lost up to 50% or more, and the yield and quality of potato is seriously affected.Over nearly 5 years, potato is yellow
Disease of withering is reported on Xinjiang of China, Hebei, Gansu, the Inner Mongol, Shandong and other places.In order to cater to the need in potato market
It wants, it is single in the upper potato planting type of production in recent years, in addition highdensity sequential cropping cultivation and the effective crop rotation of shortage are planted
Training mode causes potato verticillium wilt developmenting spread fast speed, so that crop Severe Reduction be caused even to have no harvest, seriously affects
Production estimation.Therefore, the fungicide for targetedly developing prevention and treatment potato verticillium wilt has become in agricultural production
Urgent need.
Method common in the art using chemical bactericide prevention and treatment soil-borne disease, however existing chemical bactericide effect
Single, long-time service causes pathogen resistance to enhance, while also will cause ecological environment and seriously polluting, directly in increase agricultural product
Residual, serious harm is brought to human health.Microbial bactericide is with its environment friendly and adaptable in agricultural production
In get growing concern for.Due to the complexity of soil ecology, microbial bactericide is mixed with chemical bactericide, in bacterium medicine
Interaction in, chemical bactericide whether weaken microbial bactericide colonize and survival ability, microbial bactericide are
No to generate degradation to chemical bactericide, so that chemical bactericide is compounded with microbial bactericide, there are numerous not to know
Property, it can all influence control efficiency.Therefore how to develop with proper use of microbial bactericide is the important of field of biological control
Component part.
At this stage, correlative study and product of the microbial bactericide applied to potato verticillium wilt are less, and lack correct
The operation standard effectively prevented is directed to potato verticillium wilt safely and effectively microorganism kills so needing us and developing one kind
Microbial inoculum, to cope with the present market demand.
Summary of the invention
In order to solve the above problems existing in the present technology, the present invention provides a kind of micro- lifes for preventing and treating potato verticillium wilt
The active constituent of object seed treatment, the microorganism seed inorganic agent of the prevention and treatment potato verticillium wilt is bacillus subtilis
NCD-2 and bacillus amyloliquefaciens Ba-2 and its metabolite, above-mentioned bacterial strains have efficiently, antimicrobial spectrum it is wide, using it is simple, at
The features such as this is low, non-environmental-pollution, can be used for the prevention and treatment of the soil-borne diseases such as various crop verticillium wilt, damping-off, wilt disease.Furthermore
Microorganism seed inorganic agent of the present invention also contains dispersing agent, wetting agent and filler, and it is appropriate that each material component is carried out
Weight proportion is finally prepared the seed treatment with prevention and treatment verticillium wilt, effectively avoids existing chemical bactericide effect list
One, the drawbacks such as the enhancing of pathogen resistance, the uncertainty that chemical bactericide is compounded with biocontrol microorganisms are used for a long time.
Bacillus subtilis NCD-2 and bacillus amyloliquefaciens Ba-2 of the present invention are by Hebei Prov. Academy of Agricultural &. Forest Sciences plant
Protective strategy screens acquisition.The bacillus subtilis NCD-2 is preserved in Chinese microorganism strain on October 15th, 2003
Preservation administration committee common micro-organisms center, deposit number are CGMCC No.1019；Classification system are as follows: Bacillus
Subtilis NCD-2 (hereinafter referred to as NCD-2).It is micro- to be preserved in China on June 29th, 2018 by bacillus amyloliquefaciens Ba-2
Biological inoculum preservation administration committee common micro-organisms center, deposit number are CGMCC No.16017；Classification system are as follows:
Bacillus amyloliquefaciens Ba-2 (hereinafter referred to as Ba-2).
The technical scheme adopted by the invention is as follows:
A kind of microorganism seed inorganic agent for preventing and treating potato verticillium wilt, active constituent includes bacillus subtilis NCD-
2 and bacillus amyloliquefaciens Ba-2 and its metabolite.
Further, the bacillus subtilis NCD-2 and bacillus amyloliquefaciens Ba-2 is its thallus and/or its metabolism
Product.
Further, in the active constituent, the bacillus subtilis NCD-2 and the bacillus amyloliquefaciens Ba-2
Weight ratio be (1-99): (99-1).
Further, in the active constituent, the bacillus subtilis NCD-2 and the bacillus amyloliquefaciens Ba-2
Weight ratio be (1-9): (9-1).
Further, the dosage form of the microorganism seed inorganic agent is pulvis, in wettable powder, suspending agent, aqua
Any one.
Further, the pulvis and wettable powder be can be arranged side by side different dosage forms: pulvis is produced in pesticidal preparations
A kind of most, most widely used dosage form is measured, pulvis is easy to manufacture and uses is mixed by a certain percentage with raw medicine and inert filler
With crushing, particle size is made to reach certain standard；Wettable powder is the dosage form to grow up on the basis of pulvis,
Its performance is better than pulvis, is made after being mixed well and crushed in proportion with pesticide original medicine and inert filler and a certain amount of auxiliary agent
The fine powder for reaching certain mass standard obtained.
Further, raw material components include dry bacterium powder 10-80 parts by weight, dispersing agent 1-8 parts by weight, wetting agent 1-8 weight
Measure part, filler 10-80 parts by weight.
Further, the dispersing agent is lignin sulfonic acid sodium salt, sanlose, desugar lignin sulfonic acid
One of sodium, ethylene glycol monobutyl ether, polyoxyethanyl alkyl ether, neopelex or a variety of mixtures.
Further, the wetting agent is nekal, fatty alcohol polyoxyethylene ether, dodecyl benzene sulfonic acid
One of sodium salt or a variety of mixtures.
Further, the filler is white carbon black, in kaolin, diatomite, precipitated calcium carbonate, talcum powder, bentonite
One or more mixtures.
The microorganism seed inorganic agent wettable powder of the prevention and treatment potato verticillium wilt is made with the following method:
(1) bacillus subtilis NCD-2 or bacillus amyloliquefaciens Ba-2 are activated, picking single colonie is inoculated in tiltedly
It is cultivated to obtain lawn on the culture medium of face；
(2) lawn described in sterile water washing, obtains bacteria suspension, and the bacterial suspension inoculation is carried out in fermentation medium
Shaken cultivation obtains fermentation liquid；
(3) kaolin is added in fermentation liquid to be spray-dried, obtain dry bacterium powder；
(4) each component mixed according to the proportion, stirred, level-one crushing, second level crushing, obtaining the prevention and treatment horse
The microorganism seed inorganic agent wettable powder of bell potato verticillium wilt.
Further, in step (1), cultivation temperature is 35-39 DEG C on Yu Suoshu slant medium.
Further, in step (2), the volume ratio of the bacteria suspension and fermentation medium is (1-10): 100.
Further, in step (2), the cultivation temperature is 28-32 DEG C, and the shaken cultivation speed is 170-
210rpm, the shaken cultivation time are 22-25h.
Further, in step (2), the ingredient of the fermentation medium: cane sugar content 2.5%-3.5%, beancake powder
Content is that 1.5%-2.5%, NaCl content are 0.1%-0.2%, CaCO3Content is 0.2%-0.3%, KH2PO4Content is
0.01%-0.03%, MgSO47H2O content is 0.02%-0.04%.
Further, in step (2), the total viable count of the fermentation liquid is between 5.0 × 109-1.0×1010CFU/mL it
Between.
Further, in step (3), the kaolinic addition quality is the 10%-15% of fermentation liquid.
Further, in step (3), the injector temperature of the spray drying is 170-175 DEG C, and outlet temperature is
55-60 DEG C, sample introduction speed is 30-50Hz.
Further, in step (3), the total viable count of the dry bacterium powder is between 1.5 × 1010-2.0×1010CFU/g
Between.
Further, in step (4), gained powder partial size is 60-100 μm after the level-one crushes, and the second level crushes
For air-flow crushing, gained powder partial size is 20-40 μm.
Further, in step (4), the microorganism seed inorganic agent wettable powder of the prevention and treatment potato verticillium wilt
Total viable count is between 1.5 × 109-3.0×109Between CFU/g.
Further, the microorganism seed inorganic agent of the prevention and treatment potato verticillium wilt is in prevention and treatment potato verticillium wilt
Using.
Further, the seed dressing ratio of the microorganism seed inorganic agent and seed potato of the prevention and treatment potato verticillium wilt
For 2:(75-100)；It is sowed after being placed 0-2 days at aeration-drying after the seed dressing.
The invention has the benefit that
The microorganism seed inorganic agent of prevention and treatment potato verticillium wilt of the present invention, by using bacillus subtilis
NCD-2 and bacillus amyloliquefaciens Ba-2 enables the seed treatment to colonize plant roots rapidly as active constituent
It encloses, by infecting the effects of site, secretion Substance and promotion plant nutrient absorb with the competition of verticillium wilt pathogen, most
Play to big degree Biocontrol microorganism induction disease resistance of plant and the persistently ability of control disease, to verticillium wilt pathogen have compared with
High inhibition effect.
Seed treatment of the present invention can reduce the access times and dosage of chemical bactericide, reduce cost；Delay
The generation of drug resistance, nontoxic, non agricultural chemical residuum are environmental-friendly；Ecological Distribution of Soil Microorganisms fauna is adjusted, plant growth is promoted, and
With production-increasing function；In the selection of dispersing agent, wetting agent and filler, the antagonism between microorganism is fully taken into account, collaboration is imitated
Answer and production practices on seed potato processing requirement；It can be used for dressing seed, trickle irrigation use, can rationally be made according to different situations
With saving manpower and use cost；The seed treatment preparation method is simple, at low cost, biological safety is high simultaneously, is suitable for work
Industry metaplasia produces.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.
Figure 1A is the biocompatibility measurement result of bacillus subtilis NCD-2 and filler；
Figure 1B is the biocompatibility measurement result of bacillus amyloliquefaciens Ba-2 and filler.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical solution of the present invention will be carried out below
Detailed description.Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art are obtained all without making creative work
Other embodiment belongs to the range that the present invention is protected.
Embodiment 1
The present embodiment provides a kind of microorganism seed inorganic agent and preparation method for preventing and treating potato verticillium wilt, specific implementations
Scheme is as follows:
Active constituent 10g, i.e. bacillus subtilis NCD-2 bacterial strain 1g, bacillus amyloliquefaciens Ba-2 bacterial strain 9g；
Dispersing agent 1g, specially lignin sulfonic acid sodium salt 0.3g, sanlose 0.3g, desugar lignin sulfonic acid
Sodium 0.4g；
Wetting agent 1g, specially nekal 0.5g, fatty alcohol polyoxyethylene ether 0.5g；
Filler 10g, specially white carbon black 4g, kaolin 4g, diatomite 2g.
(1) bacillus subtilis NCD-2 and bacillus amyloliquefaciens Ba-2 that -40 DEG C save are activated respectively, point
Other picking single colonie, which is inoculated on slant medium, is cultivated to obtain lawn, and cultivation temperature is 35 DEG C；
(2) it washs the lawn respectively with sterile water, obtains bacteria suspension；Fermentation medium: sugarcane is prepared according to following proportion
Sugared content is 2.5%, soya-bean cake powder content is 1.5%, NaCl content is 0.1%, CaCO3Content is 0.2%, KH2PO4Content is
0.01%, MgSO47H2O content is 0.02%, water content 95.67%；The bacteria suspension for being respectively 1/100 by volume fraction
It is inoculated in fermentation medium, with speed for 170rpm shaken cultivation 22h, cultivation temperature is 28 DEG C, obtains fermentation liquid；
(3) kaolin that mass fraction is 1/10 is added in the fermentation liquid of two kinds of bacterial strains respectively to be spray-dried, is sprayed
The dry injector temperature of mist is 170 DEG C, and outlet temperature is 55 DEG C, and sample introduction speed is 30Hz, obtains bacillus subtilis
NCD-2 bacterial strain dry powder and bacillus amyloliquefaciens Ba-2 bacterial strain dry powder；
(4) by the bacillus subtilis NCD-2 bacterial strain dry powder, bacillus amyloliquefaciens Ba-2 bacterial strain dry powder, dispersing agent,
Wetting agent and filler are successively mixed according to the proportion, are stirred, and gained powder partial size is 60 μm after level-one crushes, and then carry out gas
Stream crushes, and gained grain fineness is 20 μm, obtains the microorganism seed inorganic agent of the prevention and treatment potato verticillium wilt, total viable count
It is 1.5 × 109CFU/g；
It weighs 4kg seed treatment and 150kg seed potato mixes, be placed at aeration-drying after placing 1 day and broadcast
Kind.
Embodiment 2
The present embodiment provides a kind of microorganism seed inorganic agent and preparation method for preventing and treating potato verticillium wilt, specific implementations
Scheme is as follows:
Active constituent 80g, i.e. bacillus subtilis NCD-2 bacterial strain 72g, bacillus amyloliquefaciens Ba-2 bacterial strain 8g；
Dispersing agent 8g, specially ethylene glycol monobutyl ether 3g, polyoxyethanyl alkyl ether 3g, neopelex 2g；
Wetting agent 8g, specially fatty alcohol polyoxyethylene ether 4g, pelopon A 4g；
Filler 80g, specially precipitated calcium carbonate 30g, talcum powder 30g, bentonite 20g.
(1) bacillus subtilis NCD-2 and bacillus amyloliquefaciens Ba-2 that -40 DEG C save are activated respectively, point
Other picking single colonie, which is inoculated on slant medium, is cultivated to obtain lawn, and cultivation temperature is 35-39 DEG C；
(2) it washs the lawn respectively with sterile water, obtains bacteria suspension；Fermentation medium: sugarcane is prepared according to following proportion
Sugared content is 3.5%, soya-bean cake powder content is 2.5%, NaCl content is 0.2%, CaCO3Content is 0.3%, KH2PO4Content is
0.03%, MgSO47H2O content is 0.04%, water content 93.43%；The bacteria suspension that volume fraction is 1/10 is connect respectively
Kind is in fermentation medium, and with speed for 210rpm shaken cultivation 25h, cultivation temperature is 32 DEG C, obtains fermentation liquid；
(3) kaolin that mass fraction is 3/20 is added in the fermentation liquid of two kinds of bacterial strains respectively to be spray-dried, is sprayed
The dry injector temperature of mist is 175 DEG C, and outlet temperature is 60 DEG C, and sample introduction speed is 50Hz, obtains bacillus subtilis
NCD-2 bacterial strain dry powder and bacillus amyloliquefaciens Ba-2 bacterial strain dry powder；
(4) by the bacillus subtilis NCD-2 bacterial strain dry powder, bacillus amyloliquefaciens Ba-2 bacterial strain dry powder, dispersing agent,
Wetting agent and filler are successively mixed according to the proportion, are stirred, and gained powder partial size is 100 μm after level-one crushes, and are then carried out
Air-flow crushing, gained powder partial size are 40 μm, obtain the microorganism seed inorganic agent of the prevention and treatment potato verticillium wilt, and viable bacteria is total
Number is 3.0 × 109CFU/g；
It weighs 4kg seed treatment and 150kg seed potato mixes, be placed at aeration-drying after placing 1 day and broadcast
Kind.
Embodiment 3
The present embodiment provides a kind of microorganism seed inorganic agent and preparation method for preventing and treating potato verticillium wilt, specific implementations
Scheme is as follows:
Active constituent 20g, i.e. bacillus subtilis NCD-2 bacterial strain 10g, bacillus amyloliquefaciens Ba-2 bacterial strain 10g；
Dispersing agent 4g, lignin sulfonic acid sodium salt 1g, sanlose 1g, desugar sodium lignin sulfonate 1g, second two
Alcohol single-butyl ether 0.2g, polyoxyethanyl alkyl ether 0.2g, neopelex 0.6g；
Wetting agent 1.5g, nekal 0.5g, fatty alcohol polyoxyethylene ether 0.5g, pelopon A
0.5g；
Filler 65g, white carbon black 10g, kaolin 10g, diatomite 10g, precipitated calcium carbonate 10g, talcum powder 10g, bentonite
15g。
(1) bacillus subtilis NCD-2 and bacillus amyloliquefaciens Ba-2 that -40 DEG C save are activated respectively, point
Other picking single colonie, which is inoculated on slant medium, is cultivated to obtain lawn, and cultivation temperature is 37 DEG C；
(2) it washs the lawn respectively with sterile water, obtains bacteria suspension；Fermentation medium: sugarcane is prepared according to following proportion
Sugared content is 3%, soya-bean cake powder content is 2%, NaCl content is 0.15%, CaCO3Content is 0.25%, KH2PO4Content is
0.02%, MgSO47H2O content is 0.03%, water content 94.55%；The bacteria suspension that volume fraction is 1/20 is connect respectively
Kind in fermentation medium, with speed be 190rpm shaken cultivation for 24 hours, cultivation temperature is 30 DEG C, obtains fermentation liquid；
(3) kaolin that mass fraction is 13/100 is added in the fermentation liquid of two kinds of bacterial strains respectively to be spray-dried,
The injector temperature of spray drying is 173 DEG C, and outlet temperature is 57 DEG C, and sample introduction speed is 40Hz, obtains bacillus subtilis
NCD-2 bacterial strain dry powder and bacillus amyloliquefaciens Ba-2 bacterial strain dry powder；
(4) by the bacillus subtilis NCD-2 bacterial strain dry powder, bacillus amyloliquefaciens Ba-2 bacterial strain dry powder, dispersing agent,
Wetting agent and filler are successively mixed according to the proportion, are stirred, and gained powder partial size is 80 μm after level-one crushes, and then carry out gas
Stream crushes, and gained powder partial size is 30 μm, obtains the microorganism seed inorganic agent of the prevention and treatment potato verticillium wilt, total viable count
It is 2.0 × 109CFU/g；
It weighs 4kg seed treatment and 150kg seed potato mixes, be placed at aeration-drying after placing 1 day and broadcast
Kind.
Experimental example
One, the biocompatibility of bacillus subtilis NCD-2 and bacillus amyloliquefaciens Ba-2 and filler measures
Select diatomite, kaolin, talcum powder, clay (being indicated respectively with T1, T2, T3 and T4) representational filler into
Row screening.By different fillers respectively with bacillus subtilis NCD-2 and bacillus amyloliquefaciens Ba-2 bacterial strain raw medicine with 1:1
(w/w) ratio places 15d after mixing, using the processing for being not added with filler as blank control, is examined using the method for plate culture count
Survey thallus number.Result of study is as shown in Figure 1, Figure 1A is different fillers and bacillus subtilis NCD-2 bacterial strain biocompatibility
Measurement result, Figure 1B are different fillers and bacillus amyloliquefaciens Ba-2 bacterial strain biocompatibility measurement result, are shown: withered grass bud
Spore bacillus NCD-2 and bacillus amyloliquefaciens Ba-2 bacterial strain grow it and blank control phase with above-mentioned filler processing different time
Than significant difference is not present, there is preferable biocompatibility.
Two, influence of the seed treatment difference dosage to emergence rate
Test in March in January, 2018-Inst. of Plant Protection, Hebei-Prov. Academy of Agricultural and Forestry Scie (115 ° 30 ' 51 " E, 38 °
51 ' 33 " N) carry out.It is tested using 3 gained seed treatment of embodiment.As follows, T1:1kg/667m is arranged in test2Seed dressing；
T2:2kg/667m2Seed dressing；T3:3kg/667m2Seed dressing；T4:4kg/667m2Seed dressing；T5:5kg/667m2Seed dressing；T6: untreated
Potato seed is blank control.3 repetitions of each processing, experimental plot random alignment.Record sowing potato wedge number, when blank control all sprouts
Each processing cell number of emerging is investigated when seedling, calculates emergence rate.Different disposal emergence is investigated, the results are shown in Table 1.
1 seed treatment difference of table is administered influence of the dosage to potato emergence rate
1 kind of table can be seen that seed treatment dosage of the present invention in 1-5kg/667m2Emergence is pacified in range
Entirely.
Three, the safety evaluatio of seed treatment storage
Test in June in April, 2018-Inst. of Plant Protection, Hebei-Prov. Academy of Agricultural and Forestry Scie (115 ° 30 ' 51 " E, 38 °
51 ' 33 " N) carry out.It is tested using 3 gained seed treatment of embodiment.Microbial inoculum 4kg/ is evaluated by indoor pot experiment
667m2The safety that different time emerges to potato is stored after seed dressing.Test is provided that seed treatment according to 4kg/
667m2It is sowed after being stored 7,6,5,4,3,2,1,0 days after seed dressing in same time, setting is not simultaneously to mix on the day of sowing
The potato seed of kind is blank control.Record sowing potato wedge number, each processing cell number of emerging is investigated when blank control tidy seedlings output, is calculated
Emergence rate.It the results are shown in Table 2.
The storage safety of 2 seed treatment of table
From Table 2, it can be seen that being advisable after seed treatment processing potato seed with sowing after storing 1-2 days, should not store up
It deposited for a long time.
Four, seed treatment evaluates the control efficiency and effect of increasing production of potato verticillium wilt
It tests and is carried out October in April, 2017-in old shack township, Chengde City, Hebei Province Weichang Manchu and Mongolian Autonomous County.Make
With 3 gained seed treatment of embodiment, and potato seed mu dosage is 150-200kg.Experimental plot soil is sandy loam, and topography is flat
Whole, water and fertilizer condition is preferable.Three processing of test setting, specific as follows, T1:4kg/ mus of seed dressings；Operating procedure is as follows: by potato
Potato seed stripping and slicing guarantees 1-2 bud eye of each potato wedge, according to potato seed amount and seed dressing (150:4 or 200:4, w/w) ratio, by it
It uniformly spreads to potato seed, stirs and stir and evenly mix, make seed dressing uniform adhesion in potato seed.It is placed in ventilating and cooling place, it is spare.T2:
150g thiophanate methyl+14g Zhongshengmycin+4kg talcum powder handles potato seed；T3: potato seed is blank control without any processing.
Potato verticillium wilt grade scale is as follows:
0 grade: healthy plant；
The plant of 1 grade: 25% blade morbidity；
The plant of 2 grades: 25%-50% blade morbidity；
The plant of 3 grades: 50%-75% blade morbidity；
4 grades: 75% withered with the plant of blade morbidity or plant.
Different disposal plant incidence is investigated, disease index and control efficiency are calculated.The results are shown in Table 3.
3 seed treatment of table evaluates the control efficiency and effect of increasing production of potato verticillium wilt
From table 3 it is observed that the seed treatment to the preventive effect of potato verticillium wilt 55% or more, mu rate of growth
It is 14.18%.
Five, seed treatment evaluates the control efficiency and effect of increasing production of potato verticillium wilt
Test in October in May, 2018-the township Cheng Zi, Chengde City, Hebei Province Weichang Manchu and Mongolian Autonomous County peach mountain village into
Row.Using 3 gained seed treatment of embodiment, and potato seed mu dosage is 150-200kg.Experimental plot soil is sandy loam, ground
Gesture is smooth, and water and fertilizer condition is preferable.Two processing of test setting, specific as follows: T1:4kg/ mus of seed dressings, operating procedure are same as above；T2:
Potato seed is dressed seed with 100mL/ mus of fludioxonil.
4 seed treatment of table evaluates the control efficiency and effect of increasing production of potato verticillium wilt
As can be seen from Table 4, the seed treatment is 46.16% to the preventive effect of potato verticillium wilt, mu rate of growth
It is 8.03%.
Six, seed treatment evaluates the control efficiency and effect of increasing production of potato verticillium wilt
It is tested October in May, 2018-in decorated archway township, Chengde City, Hebei Province Weichang Manchu and Mongolian Autonomous County Hong-shi-la-zi type
Village carries out.Using 3 gained seed treatment of embodiment, and potato seed mu dosage is 150-200kg.Experimental plot soil is husky earth
Soil, topography is smooth, and water and fertilizer condition is preferable.Two processing of test setting, specific as follows, T1:4kg/ mus of seed dressings；Operating procedure is same
On.T2: potato seed is blank control without any processing.
5 seed treatment of table evaluates the control efficiency and effect of increasing production of potato verticillium wilt
As shown in Table 5, the seed treatment is 48.58% to the preventive effect of potato verticillium wilt, and mu rate of growth is
5.67%.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. a kind of microorganism seed inorganic agent for preventing and treating potato verticillium wilt, which is characterized in that its active constituent includes withered grass bud
Spore bacillus NCD-2 and bacillus amyloliquefaciens Ba-2.
2. the microorganism seed inorganic agent of prevention and treatment potato verticillium wilt according to claim 1, which is characterized in that described withered
Careless bacillus NCD-2 and bacillus amyloliquefaciens Ba-2 is its thallus and/or its metabolite.
3. the microorganism seed inorganic agent of prevention and treatment potato verticillium wilt according to claim 1, which is characterized in that the work
Property ingredient in, the weight ratio of the bacillus subtilis NCD-2 and the bacillus amyloliquefaciens Ba-2 is (1-99): (99-
1)。
4. the microorganism seed inorganic agent of prevention and treatment potato verticillium wilt according to claim 1, which is characterized in that the work
Property ingredient in, the weight ratio of the bacillus subtilis NCD-2 and the bacillus amyloliquefaciens Ba-2 is (1-9): (9-1).
5. the microorganism seed inorganic agent of prevention and treatment potato verticillium wilt according to claim 1, which is characterized in that described micro-
The dosage form of biological seed inorganic agent is pulvis, wettable powder, suspending agent, any one in aqua.
6. the microorganism seed inorganic agent of prevention and treatment potato verticillium wilt according to claim 1, which is characterized in that raw material group
Divide and includes:
Dry bacterium powder 10-80 parts by weight, dispersing agent 1-8 parts by weight, wetting agent 1-8 parts by weight, filler 10-80 parts by weight.
7. the microorganism seed inorganic agent of prevention and treatment potato verticillium wilt according to claim 6, which is characterized in that described point
Powder is lignin sulfonic acid sodium salt, sanlose, desugar sodium lignin sulfonate, ethylene glycol monobutyl ether, polyoxy second
One of base alkyl ether, neopelex or a variety of mixtures；
The wetting agent is one of nekal, fatty alcohol polyoxyethylene ether, pelopon A
Or a variety of mixture；
The filler is one of white carbon black, kaolin, diatomite, precipitated calcium carbonate, talcum powder, bentonite or a variety of
Mixture.
8. preventing and treating the microorganism seed inorganic agent of potato verticillium wilt according to claim 5 and 6, which is characterized in that institute
Wettable powder is stated to be made with the following method:
(1) bacillus subtilis NCD-2 or bacillus amyloliquefaciens Ba-2 are activated, picking single colonie is inoculated in inclined-plane training
It supports and is cultivated to obtain lawn on base；
(2) lawn described in sterile water washing, obtains bacteria suspension, and the bacterial suspension inoculation is vibrated in fermentation medium
Culture, obtains fermentation liquid；
(3) kaolin is added in fermentation liquid to be spray-dried, obtain dry bacterium powder；
(4) each component mixed according to the proportion, stirred, level-one crushing, second level crushing, obtaining the prevention and treatment potato
The microorganism seed inorganic agent wettable powder of verticillium wilt.
9. the preparation method of the microorganism seed inorganic agent of prevention and treatment potato verticillium wilt according to claim 8, feature
It is, in step (2), the total viable count of the fermentation liquid is between 5.0 × 109-1.0×1010Between CFU/mL；
In step (3), the total viable count of the dry bacterium powder is between 1.5 × 1010-2.0×1010Between CFU/g；
In step (4), it is described prevention and treatment potato verticillium wilt microorganism seed inorganic agent wettable powder total viable count between
1.5×109-3.0×109Between CFU/g.
10. application of the microorganism seed inorganic agent of the prevention and treatment potato verticillium wilt in prevention and treatment potato verticillium wilt.
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