Soil continuous cropping obstacle diseased soil disinfection water-soluble powder and application
Technical Field
The invention relates to a soil biotechnology, in particular to water-soluble powder for disinfecting soil with continuous cropping obstacle and application thereof.
Background
The continuous cropping obstacle is the phenomenon that the yield and the quality of crops decrease year by year when the same crop is continuously planted for many years in the agricultural production process. Modern scientific and technical means research shows that the root cause of continuous cropping obstacles is mass propagation of soil-borne pathogenic bacteria and root-knot nematodes, so that soil-borne diseases are increased, different degrees of mortality rate is caused by infecting crops, soil acidification, secondary salinization and nutrient supply imbalance are caused, and the growth of the crops is hindered due to accumulation of allelochemicals.
The existing technical means for eliminating the continuous cropping obstacle generally comprise the steps of adopting pesticides to combine with high-temperature greenhouse closing, steam sterilization, flooding, applying biological organic fertilizer, biological bacterial fertilizer and biological agent, an anaerobic strong reduction method and the like. Wherein, the anaerobic strong reduction method is abbreviated as RSD method. Physical means such as pesticide shed closing or steam flooding can not control and change the growth environment of soil-borne pathogenic bacteria, and the effect of preventing and controlling continuous cropping obstacles of crops with multiple causes is not ideal. In the RSD method soil remediation technology, a large amount of molasses is used, 500 yuan per ton is consumed for packaging, and more than 50% of the weight of the molasses is water, so that the transportation cost is increased sharply. Secondly, molasses is a very viscous semi-fluid liquid, is inconvenient to use, is troublesome to take out of a package or spread on the ground, has low working efficiency, and most of workers at the same line cannot be concerned about the activity.
Molasses is a byproduct of sugar mills and even a waste or a leftover of some food processing plants, and plays an important role in participating in the process of disinfecting diseased soil. The presence of moisture, however, has led to a dramatic increase in transportation costs. However, it has not been investigated whether the dry molasses could be used in place of the dry molasses for the same effect, and what side effects could be produced. Therefore, it is necessary to intensively study this problem and the related effects.
Disclosure of Invention
The invention aims to solve the problems and provides the water-soluble powder for disinfecting the diseased soil with the continuous cropping obstacles and the application thereof.
The invention provides a soil continuous cropping obstacle disease soil disinfection water-soluble powder, which is characterized in that liquid molasses is dried and crushed to obtain molasses powder, or one or more of brown sugar, white sugar, brown granulated sugar and potassium fulvate can be dissolved in water. The molasses powder may also be used in combination with one or more of brown sugar, white sugar, brown granulated sugar and potassium fulvate.
The moisture content of the molasses powder is lower than 12%.
The liquid molasses is a byproduct of a sugar refinery or a waste material produced by the sugar refinery, and has water content of more than 40%.
Wherein the brown sugar, white sugar, brown granulated sugar and potassium fulvate can be in the form of powder or granule, and the solid form is powder when used, and the water-soluble form is powder, granule or block agent when used.
The water soluble powder can be used in two ways, the first is facility agriculture, such as facility cultivation. The method is based on dry soil (the dry soil refers to soil which can not be agglomerated after being pinched and released by hands), and the dry soil is dissolved in water and uniformly sprayed into soil to be repaired according to the proportion of 30-40 tons of water, molasses powder or potassium fulvate per mu of 200 kg/mu, or 80-120 kg/mu when one or more of white sugar, brown granulated sugar and red granulated sugar is/are adopted, and the specific operation steps are as follows:
(1) pre-ploughing a diseased soil farmland;
(2) ditching on the ploughed land block, and forming a ridge between two ditches;
(3) spraying a certain amount of water-soluble powder aqueous solution on the ridge;
(4) covering the ground ridge with mulching film to isolate the ground ridge from air;
(5) uncovering the film after 15-30 days;
(6) when the soil of the ridge is semi-dry and not moist, the second plowing is carried out, and continuous cropping crops are planted. The semi-dry and non-humid state means that soil is not muddy and is suitable for plowing.
In the step (1), the residual straws and root systems of the crops are picked out.
In the step (2), the depth of the furrows at the two sides of the ridge is 30-40cm, the width of the furrows is 20-30cm, and the sum of the widths of the ridge and the furrows at the two sides is less than or equal to the width of the mulching film covered.
In the step (4), the mulching films are pressed on the mulching films in the ditches by soil, the mulching films are kept to tightly cover the ridge, one ridge is covered by one piece of mulching film, when one piece of mulching film is not enough to cover, a plurality of mulching films are used, and the width of the overlapping part between two adjacent mulching films is not less than 30cm.
When the film is uncovered, the film pressing soil is cleaned and is prevented from being mixed with the ridge soil, so that secondary pollution is avoided.
Or when the mulching film is removed, the mulching film in the trench is kept in place and is cut off only by scissors.
For the sick soil with poor granular structure and easy hardening, water-soluble powder is used for treating and disinfecting according to the method in the first year, and a proper amount of straw powder is added and removed before the second plowing, and the sick soil is buried in the soil during plowing.
The second usage of the invention is to disinfect the ill soil caused by the incomplete facilities of agriculture including open-air cultivated land, such as scattered land blocks or continuous cropping obstacles of farmlands of farmers, and the scattered farmers are big heads of agricultural cultivated land in China. The invention comprises the following steps of:
(1) ploughing the continuous cropping land;
(2) ditching and forming ridges, and watering the ground ridges to half of the saturation amount;
(3) the water soluble powder is uniformly spread on the surface of the ridge,
(4) watering for the second time to reach a saturated amount;
(5) covering the ground ridge with mulching film to isolate it from air;
(6) uncovering the film after 15-30 days;
(7) and when the soil is cooled to be half-dry, turning over for the second time, and carrying out continuous planting on the crops.
In the step (6), if the film is continuously removed in sunny days, the film can be removed in 15 days, the film is removed in rainy days and 30 days, and the weather between sunny days and cloudy days is properly selected in 15-30 days. The saturation in steps (2) and (4) means that 30-40 tons/mu of dry soil is watered.
For the cultivated land with poor granular structure and hardened land, the straw powder of crops can be combined for treatment, and the specific method is that the straw of non-continuous cropping crops is scattered on the ground during the second plowing and is buried in the soil.
The invention relates to a method for disinfecting soil with continuous cropping obstacle, which is innovation and development of an RSD method. Practice proves that after the method is adopted, the nitrogen element in the soil is consumed more, the original balance of the fertilizer elements can be broken, the growth of crops is weak, and 25 kg/mu of nitrogen fertilizer or slow-release urea is recommended to be used during the second turnover so as to make up for the lack of the nitrogen fertilizer.
Practice shows that compared with liquid molasses, the water soluble powder for repairing and disinfecting the continuous cropping obstacle diseased soil has the same or even better technical effect than molasses in the RSD method under the same condition, particularly has quite obvious sterilization rate on fusarium oxysporum, has less than half of the dosage of the liquid molasses, greatly reduces the volume and the weight, reduces the transportation cost and is convenient to use. The total use cost is reduced by more than 30 percent. Therefore, the invention thoroughly solves the problems of high transportation difficulty and poor safety and use difficulty of the liquid molasses caused by expansion with heat and contraction with cold.
Drawings
FIG. 1 is a graph showing the effect of RSD treatment of continuous cropping obstacle of Acacia mangium in a sugar refinery in Yunnan province.
FIG. 2 is a graph showing the effect of RSD treatment of soil with continuous cropping obstacles of rare and mature places in Jiangsu province.
FIG. 3 is a diagram of the effect of treating the local lily continuous cropping obstacle disease soil with Kunming cluster by using a RSD method instead of molasses powder.
FIG. 4 is a diagram showing the effect of white sugar instead of molasses powder RSD on treating soil with continuous cropping obstacle of Yunnan Shipingyan radix Platycodi.
FIG. 5 is a graph showing the sterilization effect of red granulated sugar instead of molasses powder RSD method on the soil with continuous cropping obstacle of Abelmoschus martensii tomato.
FIG. 6 is a graph showing the effect of brown granulated sugar instead of molasses powder RSD on the salinity of the soil with continuous cropping obstacles of Abelmoschus martensii tomato.
FIG. 7 shows the photo of 8-year old foreign straw stalk diseased soil cultivated in the unit of the applicant.
FIG. 8 is a photograph of a repaired object made by 8 years old corn stalk disease soil molasses powder replacing liquid molasses RSD in the unit of the applicant.
FIG. 9 shows photographs of soil-cultivated species with Adina rubella disease for 5 years in a certain place of Yunnan.
FIG. 10 is a photograph of a repaired real object of 5-year-old Sasa quefolius soil white sugar replacing liquid molasses RSD in a certain place of Yunnan.
FIG. 11 is a real object photograph of a 6-year carnation disease soil cultivated in a certain place of Yunnan.
FIG. 12 is a real photograph of a 6-year carnation disease soil brown sugar in a certain place of Yunnan after being repaired by replacing liquid molasses RSD.
FIG. 13 is a contemporary physical control of potassium fulvate replacing liquid molasses RSD to repair a continuous variety of 4 year lily in a certain location.
FIG. 14 is a real comparison of contemporary root system of a continuous 4-year lily in a certain place repaired by potassium fulvate instead of liquid molasses RSD.
FIG. 15 is a photograph of a control of bananas planted in a certain place for 7 years.
FIG. 16 is a photograph of repairing the continuous cropping 7-year banana land shown in FIG. 15 by replacing liquid molasses RSD with mixed brown sugar and brown granulated sugar powder.
In figure 1, sugar powder refers to molasses powder, molasses refers to liquid molasses, and original soil is untreated diseased soil.
In figure 2, sugar powder refers to molasses powder, molasses refers to liquid molasses, and original soil is untreated diseased soil.
The powder in fig. 3 is brown sugar powder, the material refers to crop straw powder (without lily straw), the liquid refers to liquid molasses, and the contrast is untreated diseased soil.
The powder in fig. 4 is white sugar powder, the material is crop straw powder (without eustoma grandiflorum), the liquid is liquid molasses, and the contrast is untreated diseased soil.
In fig. 5 and 6, the powder refers to brown sugar powder, the liquid refers to liquid molasses, the material refers to crop straw powder (without continuous crop straw), and CK refers to untreated diseased soil.
The white sugar, the brown sugar, the red land leveler and the red granulated sugar related in the figures are all the lowest-grade products.
Detailed Description
The following further illustrates the advantageous technical effects of the present invention with reference to the accompanying drawings and examples.
Example 1 waste molasses from Yunnan province was dried to obtain molasses powder with water content below 12%, and packaged in packaging bags. The same liquid molasses is used for comparative test on the disinfection of the diseased soil with continuous cropping obstacles. The molasses liquid contains 55% of water and is viscous liquid, and is packaged by a plastic can and used as a main material of an RSD method in a large amount. The comparative test cases of this example are as follows:
sources of disease soil: the number of fusarium oxysporum in the soil is 4.07 x 10 in a cultivation base of a Cooperation of Yunnan Jinning, Abies and Mei Mi
7ITS copies g
-1dry soil。
Processing and setting: the method comprises four processes: 0.046% of molasses powder (equivalent to 120 kg/mu); 0.063% molasses powder (160 kg/mu); 0.077% (200 kg/acre); 0.15% liquid molasses (400 kg/acre). The soil is calculated as 260 tons per mu.
The treatment conditions are as follows: sealing, and culturing in 35 deg.C incubator for 15 days. Sealing the soil sample under each treatment and isolating the soil sample from air, and then culturing the soil sample in an incubator at 35 ℃ for 15 days. The same applies below.
Detection indexes are as follows: honey powder TOC, TN, EOC, C/N; the treated soil has the quantity of fusarium oxysporum.
The detection method comprises the following steps: total Organic Carbon (TOC) and Total Nitrogen (TN) are respectively determined by a high-temperature heating potassium chromate oxidation-volumetric method and a Kai-shi disinfection method in soil agricultural chemistry analysis method of Luruokun, and EOC (easily oxidized organic carbon) is determined by a potassium permanganate oxidation method.
The soil DNA extraction uses a Kit MP Fast DNA Isolation Kit, and the fusarium oxysporum is quantified through real-time fluorescent quantitative PCR and corresponding specific primers.
As a result:
TOC(g/kg) TN(g/kg) EOC(g/kg) C/N
honey powder 319.3336.18158.468.83
The sterilization rate is as follows: the RSD treatment of the molasses powder and the liquid molasses can obviously kill the fusarium oxysporum, and the sterilization rate of 0.046% treatment of the molasses powder is the highest, reaches 98.5%, is 1.4% higher than that of the liquid molasses, and the application amount is less than 30% of that of the liquid molasses. See figure 1. In addition, the EC value of the soil salinity and the molasses powder are 0.527 (ms/cm) which is 0.234 metering units lower than that of the control. The effect of field treatment is shown in figure 7-8.
Example 2, waste molasses of a sugar refinery in Yunnan is dried to obtain molasses powder with 12% of water content, which is specially ordered by our unit with friends and packaged by plastic bags. The same liquid molasses is used for comparative test on the disinfection of the diseased soil with continuous cropping obstacles. The molasses liquid contains 52% of water and is a viscous liquid, and a large amount of molasses liquid is conventionally packaged in a plastic can and used as a main raw material in the RSD method.
The comparative test cases of this example are as follows:
sources of disease soil: a pepper cultivation base of a company in a perennial city is continuously cultivated into 5 diseased soil, and the number of fusarium oxysporum in the soil is 4.76 multiplied by 10
6ITS copies g
-1dry soil。
Processing and setting: 0.069% of molasses powder (equivalent to 180 kg/mu); 0.096% molasses powder (250 kg/mu); 0.15% liquid molasses (400 kg/acre). One acre of land is calculated by 260 tons of soil.
The treatment conditions are as follows: sealing, and culturing in 35 deg.C incubator for 15 days.
Detection indexes are as follows: honey powder TOC, TN, EOC, C/N; the treated soil has the quantity of fusarium oxysporum.
The detection method comprises the following steps: total Organic Carbon (TOC) and Total Nitrogen (TN) were determined by high-temperature heated potassium chromate oxidation-volumetric method and Kelvin method of soil Agrochemical analysis, Luruokun, respectively, and EOC was determined by potassium permanganate oxidation.
The soil DNA extraction uses a Kit MP Fast DNA Isolation Kit, and the fusarium oxysporum is quantified through real-time fluorescent quantitative PCR and corresponding specific primers.
As a result:
TOC(g/kg) TN(g/kg) EOC(g/kg) C/N
honey powder 415.3911.72317.4435.44
The sterilization rate is as follows: RSD treatment of the molasses powder and the liquid molasses can obviously kill fusarium oxysporum, the sterilization rate is as high as 99.2-99.7%, and the molasses powder and the liquid molasses have no obvious difference. See fig. 2.
On the basis of the success of the above tests, the applicant carried out tests for RSD with sugar instead of molasses powder at large. The following results were obtained:
example 3, commercially available brown sugar (lowest sugar) is used, after powdering, RSD treatment is carried out on diseased soil of Xiangxiang Lily of Kunming cluster of Yunnan for many years, and comparison experiments are carried out with other treatment agents. Comprises contrast of original disease soil, liquid molasses treatment, material treatment and brown sugar powder treatment.
Detection indexes are as follows: pH, EC, amount of Sphaerotheca oxysporum Sickle knife bacteria.
The detection method was the same as in example 1.
And (3) detection results:
the pH value of the soil after RSD treatment is acidic, compared with the pH value after RSD treatment, the pH value after RSD treatment is obviously increased, and the difference between different treatments is obvious, wherein the highest pH value of material treatment is 5.8, the highest pH value of liquid molasses is 5.5, brown sugar powder is 4.9, and the contrast (original disease soil) is 4.8.
The EC value, after RSD treatment, was reduced by up to 0.15, liquid molasses 0.36, brown sugar 0.62, control 0.4. The measurement units are as in example 1
Compared with a control, the quantity of the saccharomycete Sickle in the soil is obviously reduced after RSD treatment, the quantity of the saccharomycete Sickle in the RSD treatment is different, wherein the sterilization rate of the material treatment can reach 98.63 percent, and the brown sugar powder and the liquid molasses are respectively 91.86 percent and 85.73 percent. See fig. 3. The effect after field treatment is shown in fig. 13.
Example 4 RSD treatment of diseased soil of fresh eustoma grandiflorum of the applicant of shisha yunnanensis for several years with commercially available white sugar (white sugar, herein referred to as compound sugar powder or powdered sugar). The specific process is as follows:
source of disease
The number of fusarium oxysporum in soil is 3.98 multiplied by 10
8ITS copies g
-1dry soil。
Processing settings
The method comprises five processes: control (original disease soil); 0.029% powdered sugar (75 kg/acre); 0.038% powdered sugar (100 kg/mu); 0.077% powdered sugar (200 kg/acre); 0.15% powdered sugar (400 kg/mu). The soil is calculated as 260 tons per mu.
The treatment conditions are as follows: sealing, and culturing in 35 deg.C incubator for 20 days.
Detecting the index
Sugar powder TOC, TN, C/N; the treated soil has the quantity of fusarium oxysporum.
Detection method
Determination of TOC and TN of powdered sugar
Total Organic Carbon (TOC) and Total Nitrogen (TN) were measured by the high-temperature heating potassium chromate oxidation-volumetric method and the Kai digestion method, respectively, in Luruokun soil Agrochemical analysis.
Extraction of soil DNA and quantification of Fusarium oxysporum
The soil DNA extraction uses a Kit MP Fast DNA Isolation Kit, and the fusarium oxysporum is quantified through real-time fluorescent quantitative PCR and corresponding specific primers.
Results
Sugar powder TOC, TN, C/N
The TOC content of the composite sugar powder is 416.91g/kg, the TN content is 4.13 g/kg, and the C/N is 100.95.
Rate of sterilization
The RSD treatment of the composite powdered sugar can obviously kill the fusarium oxysporum in soil, and the sterilization rate of the powdered sugar is related to the addition amount of the powdered sugar, wherein 0.15% of the powdered sugar has the best sterilization effect, the sterilization rate is 96.71%, the sterilization rate of 0.077% of the powdered sugar is 94.26%, the sterilization rate of 0.038% of the powdered sugar is 88.26%, and the sterilization rate of 0.029% of the powdered sugar is the lowest, and is 85.67%. The results are shown in FIG. 4. The effect of field treatment is shown in FIGS. 7-8.
Example 5 treatment of some places in Yunnan Emei county with okra, balsam pear and tomato disease soil using fulvic acid potassium instead of sugar liquid molasses RSD. The potassium fulvate is a byproduct of a certain yeast factory and is sold as an organic fertilizer or a biological bacterium raw material externally. The specific process is as follows:
source of soil sample
The method comprises the following steps of continuously cropping disease soil of bitter gourds, okra and tomatoes in the county of Emhan county of Yuxi Yunnan, wherein the disease soil comprises contrast disease soil, powder treatment, liquid treatment and material treatment. The powder in this example is potassium fulvate, the liquid is liquid molasses, and the material is straw powder which does not contain treated continuous cropping crop straws.
Detecting the index
pH, EC, Fusarium oxysporum number.
Detection method
Determination of soil pH
The soil and deionized water are mixed evenly according to the proportion of 1:2.5, and then the soil pH is measured by a pH meter after the soil is shaken in a shaking table for 30min at the speed of 250 r/min.
Determination of soil EC
The soil and deionized water are mixed evenly in a ratio of 1:5, and then the soil EC is measured by an EC meter after shaking for 30min in a shaking table at 250 r/min.
Extraction of soil DNA and quantification of Fusarium oxysporum
The soil DNA extraction uses a Kit MP Fast DNA Isolation Kit, and the fusarium oxysporum is quantified through real-time fluorescent quantitative PCR and corresponding specific primers.
The result of the detection
pH of soil
After the balsam pear RSD treatment, the pH value of the soil is obviously increased, alkaline soil is between 7.5 and 8, and the pH value of powder treatment is obviously higher than that of liquid and material treatment; after the okra is treated by RSD, the pH value of the soil is obviously increased, and the pH value difference among different treatments is obvious; the pH value of the soil after the RSD treatment of the tomatoes is reduced, and particularly the pH value of the soil after the RSD treatment of the tomatoes is obviously reduced by powder treatment and liquid treatment.
Soil EC
Compared with a control, the soil EC is remarkably reduced after three crops are treated by RSD, the salt content is reduced, and the EC value difference among different treatments is remarkable. See figure 6 for details.
Fusarium oxysporum number in soil
After RSD treatment, the quantity of balsam pears, okra and tomato fusarium oxysporum is reduced remarkably on the whole, but the difference among different treatments is not remarkable, the treatment effect of balsam pears and okra powder is good, and the sterilization rate is 80.86% and 90.47% respectively; the tomato material has good treatment effect, and the sterilization rate is 90.02%. See figure 5 for details.
It should be noted that the RSD method mentioned in each example above refers to a process in which water-soluble powder or equal-gauge rod powder is mixed with soil for diseases in a laboratory, sealed from air, and cultured in an incubator at 35 ℃ for 15 days. In the field, the water-soluble powder or straw powder is used for the treatment process according to the using method.
The applicant unit expands the application of these experimental results to the fields of other continuous cropping crops. The effect is shown in fig. 7-16.