CN109651518A - A kind of extracting method of hare's-lettuce polysaccharide - Google Patents
A kind of extracting method of hare's-lettuce polysaccharide Download PDFInfo
- Publication number
- CN109651518A CN109651518A CN201710932405.2A CN201710932405A CN109651518A CN 109651518 A CN109651518 A CN 109651518A CN 201710932405 A CN201710932405 A CN 201710932405A CN 109651518 A CN109651518 A CN 109651518A
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- CN
- China
- Prior art keywords
- hare
- lettuce
- precipitating
- dry powder
- extracting method
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Classifications
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The invention discloses a kind of extracting methods of hare's-lettuce polysaccharide easy to operate, extraction efficiency is high.Its processing step includes: that soft rotten, putrid and deteriorated blade is rejected from the hare's-lettuce of purchase, cuts off root, and cleaning removes the impurity such as soil, sufficiently dries in drying box, then smashed with high speed Universal pulverizer, crosses 40 meshes, hare's-lettuce dry powder is made.Accurately weigh 20-30g hare's-lettuce dry powder, it puts in a round bottom flask, hot water extraction under magnetic stirring, leaching liquor 4000r/min is centrifuged, and is filtered after 10min, filtrate decompression is concentrated into the 1/4 of original volume, then sevage reagent removing protein is used, alcohol precipitation is carried out with 95% ethyl alcohol of 4 times of volumes again, 4000r/min is centrifuged 10min, obtains Thick many candies precipitating.This precipitating is washed with dehydrated alcohol, acetone, ether respectively, is dried in vacuo, obtains hare's-lettuce Thick many candies.Hare's-lettuce polysaccharide is prepared using this method, products obtained therefrom purity is high is easy to industrialized production.
Description
Technical field
The invention belongs to field of natural medicinal chemistry, in particular to a kind of extracting method of hare's-lettuce polysaccharide.
Background technique
Hare's-lettuce is dicotyledonous composite family, and 1~2 year raw herbaceous plant, also known as bitter chrysanthemum originate in Europe, now in China north and south
Fang Jun is distributed.The cold hardship of hare's-lettuce taste, can heat-clearing, detumescence, cooling blood and hemostasis, can be used for treating ecphyaditis, cervicitis, mazoitis,
The diseases such as jaundice, dysentery, protein, amino acid and the necessary microelement of human body rich in hare's-lettuce have very high reality
With value.
Summary of the invention
To meet clinical needs, expands medicine variety, preferably treat ecphyaditis, prepare anti-ecphyaditis using hare's-lettuce polysaccharide
Drug.
Technical solution of the present invention is carried out according to the following steps:
A. soft rotten, putrid and deteriorated blade is rejected from the hare's-lettuce of purchase, cuts off root, and cleaning removes the impurity such as soil, Yu Gan
It sufficiently dries in dry case, is then smashed with high speed Universal pulverizer, cross 40 meshes, hare's-lettuce dry powder is made.
B. accurately weigh 20-30g hare's-lettuce dry powder, put in a round bottom flask, under magnetic stirring hot water extraction, leaching liquor from
It is filtered after the heart, filtrate decompression is concentrated into the 1/4 of original volume.
C. sevage reagent removing protein is then used, then carries out alcohol precipitation, 4000r/min centrifugation with 95% ethyl alcohol of 4 times of volumes
10min obtains Thick many candies precipitating.
D. this precipitating is washed with dehydrated alcohol, acetone, ether respectively, is dried in vacuo, obtains hare's-lettuce Thick many candies.
Centrifugation filters 10min under the conditions of 4800r/min described in step b.
Extraction temperature described in step b is 80 DEG C.
The present invention is further illustrated below in conjunction with specific embodiment, but the scope of protection of present invention is not limited to
In following embodiments.
Specific embodiment:
Embodiment 1:
Soft rotten, putrid and deteriorated blade is rejected from the hare's-lettuce of purchase, cuts off root, cleaning removes the impurity such as soil, in drying
It sufficiently dries in case, is then smashed with high speed Universal pulverizer, cross 40 meshes, hare's-lettuce dry powder is made.Accurately weigh 20g hardship
Lettuce dry powder is put in a round bottom flask, under magnetic stirring hot water extraction, is filtered after leaching liquor centrifugation, filtrate decompression is concentrated into original
The 1/4 of volume.Then sevage reagent removing protein is used, then carries out alcohol precipitation, 4000r/min centrifugation with 95% ethyl alcohol of 4 times of volumes
10min obtains Thick many candies precipitating.This precipitating is washed with dehydrated alcohol, acetone, ether respectively, is dried in vacuo, it is slightly more to obtain hare's-lettuce
Sugar, yield 90.5%.
Embodiment 2:
Soft rotten, putrid and deteriorated blade is rejected from the hare's-lettuce of purchase, cuts off root, cleaning removes the impurity such as soil, in drying
It sufficiently dries in case, is then smashed with high speed Universal pulverizer, cross 40 meshes, hare's-lettuce dry powder is made.Accurately weigh 25g hardship
Lettuce dry powder is put in a round bottom flask, under magnetic stirring hot water extraction, is filtered after leaching liquor centrifugation, filtrate decompression is concentrated into original
The 1/4 of volume.Then sevage reagent removing protein is used, then carries out alcohol precipitation, 4000r/min centrifugation with 95% ethyl alcohol of 4 times of volumes
10min obtains Thick many candies precipitating.This precipitating is washed with dehydrated alcohol, acetone, ether respectively, is dried in vacuo, it is slightly more to obtain hare's-lettuce
Sugar, yield 92.9%.
Embodiment 3:
Soft rotten, putrid and deteriorated blade is rejected from the hare's-lettuce of purchase, cuts off root, cleaning removes the impurity such as soil, in drying
It sufficiently dries in case, is then smashed with high speed Universal pulverizer, cross 40 meshes, hare's-lettuce dry powder is made.Accurately weigh 30g hardship
Lettuce dry powder is put in a round bottom flask, under magnetic stirring hot water extraction, is filtered after leaching liquor centrifugation, filtrate decompression is concentrated into original
The 1/4 of volume.Then sevage reagent removing protein is used, then carries out alcohol precipitation, 4000r/min centrifugation with 95% ethyl alcohol of 4 times of volumes
10min obtains Thick many candies precipitating.This precipitating is washed with dehydrated alcohol, acetone, ether respectively, is dried in vacuo, it is slightly more to obtain hare's-lettuce
Sugar, yield 95.3%.
Claims (3)
1. a kind of extracting method of hare's-lettuce polysaccharide, it is characterised in that it the following steps are included:
A. soft rotten, putrid and deteriorated blade is rejected from the hare's-lettuce of purchase, cuts off root, and cleaning removes the impurity such as soil, Yu Gan
It sufficiently dries in dry case, is then smashed with high speed Universal pulverizer, cross 40 meshes, hare's-lettuce dry powder is made.
B. 20-30g hare's-lettuce dry powder is accurately weighed, is put in a round bottom flask, under magnetic stirring hot water extraction, after leaching liquor centrifugation
It filters, filtrate decompression is concentrated into the 1/4 of original volume.
C. sevage reagent removing protein is then used, then carries out alcohol precipitation, 4000r/min centrifugation with 95% ethyl alcohol of 4 times of volumes
10min obtains Thick many candies precipitating.
D. this precipitating is washed with dehydrated alcohol, acetone, ether respectively, is dried in vacuo, obtains hare's-lettuce Thick many candies.
2. a kind of extracting method of hare's-lettuce polysaccharide according to claim 1, which is characterized in that described in step b
Centrifugation filters 10min under the conditions of 4800r/min.
3. a kind of extracting method of hare's-lettuce polysaccharide according to claim 1, which is characterized in that extraction described in step b
Temperature is 80 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710932405.2A CN109651518A (en) | 2017-10-10 | 2017-10-10 | A kind of extracting method of hare's-lettuce polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710932405.2A CN109651518A (en) | 2017-10-10 | 2017-10-10 | A kind of extracting method of hare's-lettuce polysaccharide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109651518A true CN109651518A (en) | 2019-04-19 |
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ID=66108259
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710932405.2A Pending CN109651518A (en) | 2017-10-10 | 2017-10-10 | A kind of extracting method of hare's-lettuce polysaccharide |
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| CN (1) | CN109651518A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112341515A (en) * | 2020-11-20 | 2021-02-09 | 榆林学院 | Method for extracting taraxasterol from sow thistle |
-
2017
- 2017-10-10 CN CN201710932405.2A patent/CN109651518A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112341515A (en) * | 2020-11-20 | 2021-02-09 | 榆林学院 | Method for extracting taraxasterol from sow thistle |
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| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190419 |