CN107286702B - A method of separating different tone Gardenia Yellows from Gardenia Yellow dry fruit - Google Patents
A method of separating different tone Gardenia Yellows from Gardenia Yellow dry fruit Download PDFInfo
- Publication number
- CN107286702B CN107286702B CN201710632547.7A CN201710632547A CN107286702B CN 107286702 B CN107286702 B CN 107286702B CN 201710632547 A CN201710632547 A CN 201710632547A CN 107286702 B CN107286702 B CN 107286702B
- Authority
- CN
- China
- Prior art keywords
- gardenia yellow
- gardenia
- product
- dry fruit
- flow velocity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The method that the invention discloses a kind of to separate different tone Gardenia Yellows from Gardenia Yellow dry fruit, comprising the following steps: (1) be crushed: Gardenia Yellow dry fruit is chosen, selection by winnowing decladding after 1-3mm is crushed to；(2) extract: by the Gardenia Yellow after decladding using flooding 3-5 times that temperature is 30-50 DEG C, the additional amount of the water is 3-8 times of Gardenia Yellow dry fruit weight, extracts 1-2h every time, merges each leaching liquor；(3) it is centrifuged: being centrifuged using butterfly centrifugal machine, flux 200-400L/h, the microfiltration of ceramic membrane that clear liquid is 0.2 μm using aperture collects filtrate；(4) gradient elution；(5) Gardenia Yellow product A, B, C, D, E, F, G, H can be respectively obtained after being concentrated；Present invention process is simple, and production cost is low, and the product of eight kinds of tone difference can be made respectively, be precisely controlled by color difference meter, sufficiently meets the market demand, guarantees the control stability of quality between batch products.
Description
Technical field
It is specifically a kind of that different tone Cape jasmines are separated from Gardenia Yellow dry fruit the present invention relates to natural pigment separation technology field
Sub yellow method.
Background technique
Cape jasmine also known as Yellow Fructus Gardeniae, fructus gardeniae, white toad are the fruits of madder wort cape jasmine.The fruit of cape jasmine is in tradition
Medicine belongs to the l batches of dual-purpose of drug and food resources that the Ministry of Public Health promulgates, has the effects that protect liver, cholagogue, decompression, calmness, hemostasis, detumescence.?
Tcm clinical practice is usually used in treating the diseases such as icteric hepatitis, bruise, hypertension, diabetes.The base of glycosides containing safran, can make
Weld, the pigment extracted are Gardenia Yellow, and alias crocin is commonly called as Yellow Fructus Gardeniae, belong to carotenoid series, it is cape jasmine
In yellow pigment, yellow pigment ingredient be Pigment of carotenoid family crocin, it is identical as the crocin in safflower, point
Minor is C44H64O24, relative molecular mass 976.97.Gardenia Yellow can be used for fruit juice (taste) type beverage, assembled alcoholic drinks, color on cake
Adornment, ice lolly, ice cream, dilated food, jelly, flour cake, candy and chestnut can.
The raw material for extracting Gardenia Yellow at present is all using Gardenia Yellow dry fruit, the rare report extracted using fresh fruit mostly
Road.Since its dry fruit is during water-removing, dry, pigment in raw material it is different degrees of oxidation, brown stain, drop all has occurred
Solution, during the production of extraction, purifying and subsequent product, the pigments such as oxidation, brown stain, degradation enter final product together
In, though there is the purifying refining methd of more patent and document report Gardenia Yellow, all not to the pure of separation process node product
Degree and tone difference make judge.If Chinese patent notification number is CN100528970C, disclose it is a kind of separated from cape jasmine it is pure
The method for changing gardenia yellow pigment with high color value, steps of the method are: cape jasmine is extracted from Gardenia Yellow using hydrophilic organic solvent
Medicinal extract is made in uranidin, and through enriching and purifying macroporous resin, Gardenia Yellow crude product is made, and the ethyl acetate through heat extracts
It removes the impurity such as Gardenoside and chlorogenic acid, E is made4401cm 1%>=580, the height of A238/A440≤0.25, A325/A440≤0.24
Color value Gardenia Yellow.It is difficult to for Gardenia Yellow and water-solubility impurity being kept completely separate using macroporous absorbent resin in this method,
Still there is the impurity in addition to Gardenoside and chlorogenic acid, and be difficult to be removed by the ethyl acetate extraction of heat, influences the color of final products
Valence, and the patent, due to complex process, production cost is also relatively high.Chinese patent notification number is CN102911513B, open
A kind of extracting method of Gardenia Yellow, the extracting method include: A) to the cape jasmine by peeling processing crushed so as to
Cape jasmine powder is obtained, flooding, the leaching liquor mentioned are then added into cape jasmine powder；B) leaching liquor is separated by solid-liquid separation and is obtained
Supernatant, then micro-filtration is carried out to supernatant and obtains the first filtrate；C) using the Gardenia Yellow in the first filtrate of resin adsorption, so
Resin is carried out afterwards to afford eluent, D) the second filtrate is obtained to eluent progress ultrafiltration, the second filtrate is dried
Gardenia Yellow can be obtained.The patent obtains the long Gardenia Yellow of High color values, high-purity, high-quality and storage period, still
There is no separate the Gardenia Yellow of different tones according to the difference of Gardenia Yellow tone.
The Gardenia Yellow product of each producer all has a problem that currently on the market: the product of same color value, tone it is all pure
Difference can have the difference of tone during terminal Gardenia Yellow products application.As a kind of natural colorant, in addition to purity,
The difference control of color is also that it is more demanding to homogeneity of product especially to face foreign countries etc. to technique, the requirements at the higher level of product
Client.Therefore, proposed by the invention that Gardenia Yellow separation and color difference control will be to Gardenia Yellow product and work
One kind of skill control is supplemented well.
Summary of the invention
Present invention aim to solve the above problem existing for existing Gardenia Yellow, provide a kind of from Gardenia Yellow
The method that different tone Gardenia Yellows are separated in dry fruit, this method simple process, production cost is low, can be made respectively using this method
Product A, B, C, D, E, F, G, the H for obtaining eight kinds of tone difference, are precisely controlled by color difference meter, sufficiently meet the market demand, are guaranteed
The control stability of quality between batch products.
A kind of method separating different tone Gardenia Yellows from Gardenia Yellow dry fruit of the invention, comprising the following steps:
(1) it is crushed: choosing Gardenia Yellow dry fruit, be crushed to selection by winnowing decladding after 1-3mm；
(2) it extracts: the Gardenia Yellow after decladding is used into flooding 3-5 times that temperature is 30-50 DEG C, the additional amount of the water
It is 3-8 times of Gardenia Yellow dry fruit weight, extracts 1-2h every time, after the completion of extraction, merge each leaching liquor；
(3) it is centrifuged: leaching liquor is centrifuged, the microfiltration of ceramic membrane that clear liquid is 0.2 μm using aperture after centrifugation collects filtrate；
(4) it elutes: by above-mentioned filtrate with the absorption of PRP chromatographic resin, being eluted using linear gradient elution method, be first with mass fraction
10% ethanol water elution, elution volume 3-4BV, flow velocity 0.5-1BV collect eluent A；It is with mass fraction again
20% ethanol water elution, elution volume 3-4BV, flow velocity 0.5-1BV collect eluent B；Then with mass fraction
It is eluted for 30% ethanol water, elution volume 3-4BV, flow velocity 0.5-1BV collect eluent C；Then with quality point
Number elutes for 40% ethanol water, and elution volume 2-3BV, flow velocity 0.5-1BV collect eluent D；Then with quality
The ethanol water that score is 50% elutes, elution volume 2-3BV, flow velocity 0.5-1BV, collects eluent E；Then with matter
It measures the ethanol water that score is 60% to elute, elution volume 2-3BV, flow velocity 0.5-1BV collect eluent F；Then with
The ethanol water that mass fraction is 70% elutes, elution volume 2-3BV, flow velocity 0.5-1BV, collects eluent G；Then
It is eluted with the ethanol water that mass fraction is 80%, elution volume 2BV, flow velocity 0.5-1BV collect eluent H；
(5) it is concentrated: above-mentioned eluent A, B, C, D, E, F, G, H is concentrated under reduced pressure into the concentration that solid content is 30-40% respectively
Liquid, ethyl alcohol recycling, after concentrate is spray-dried, can respectively obtain Gardenia Yellow product A, B, C, D, E, F, G, H, described
The color value of Gardenia Yellow product A is greater than 100, and the color value of Gardenia Yellow product B is greater than 120, the color of Gardenia Yellow products C
Valence is greater than 340, and the color value of Gardenia Yellow product D is greater than 390, and the color value of Gardenia Yellow product E is greater than 460, cape jasmine yellow
The color value of plain product F is greater than 480, and the color value of Gardenia Yellow product G is greater than 500, and the color value of Gardenia Yellow product H is greater than
510。
Preferably, the broken partial size in heretofore described step (1) is 2mm.
Preferably, water temperature is 40 DEG C in heretofore described step (2).
Preferably, it is centrifuged in heretofore described step (3) using butterfly centrifugal machine, flux 200-400L/min.
Preferably, it is concentrated under reduced pressure in heretofore described step (5) using outer circulation type single effect evaporator.
Further, the flux of disk centrifugal separator is 300L/min in heretofore described step (3).
According to color difference meter empirical value, within dE* value is less than 4, tone difference of people's naked eyes fundamental sensation less than color, sheet
It invents obtained eight kinds of Gardenia Yellow powder products to analyze through color difference meter respectively, dE* value difference away from obvious, deposit by naked-eye observation tone
In notable difference, eight kinds of Gardenia Yellow powders are diluted to E440nm=1 with pure water respectively, tone is presented as powder
Gap.The present invention carries out the analysis of purity and color difference to technical process, the node isolated and purified, is the control of Gardenia Yellow Product Process
System, the control of finished product mass uniformity, products application etc. will have relatively good application prospect, get rid of Gardenia Yellow only with color
Valence is the sole requirement for measuring quality.The invention can track the corresponding relationship of entire production process product color difference and purity simultaneously
And difference, as a kind of natural colorant, in addition to purity, the difference control of color is also to technique, the requirements at the higher level of product, spy
It is not in face of clients more demanding to homogeneity of product such as foreign countries, Gardenia Yellow separation proposed by the present invention and color difference control will
It is to be supplemented very well one kind of Gardenia Yellow product and technology controlling and process.
Eight kinds of Gardenia Yellow products that the present invention separates, in addition to purity difference is unusual, the difference of tone is obvious, by
In Gardenia Yellow mainly as food additives (colorant), the difference of tone will directly affect the color difference of terminal coloring, cape jasmine
Xanthan material uncertainty existing for Different Harvesting Time, drying, last handling process itself, it will to the color of Gardenia Yellow product
Tune brings difference, if the consistency (tone) of product hardly results in guarantee, and the method for the present invention is isolated without color difference control
The Gardenia Yellow of eight kinds of tone difference can be precisely controlled the consistency of product shade according to customer demand, can avoid completely
There is the risk of tone difference in application process in terminal client, better meets the market demand.
The present invention has several advantages that compared with the separation method of existing Gardenia Yellow
(1) it is extracted after being crushed raw material using physical process using pure water, has abandoned ethyl alcohol and other extraction processes,
Reduce pigment loss, reduces solvent-oil ratio, environmental-friendly, energy-saving and emission-reduction；
(2) eight kinds of Gardenia Yellow product purities that the present invention obtains are high, by taking PRP chromatographic resin to combine more gradients
Elution process, product purity up to 500 color values more than.
(3) the eight kinds of Gardenia Yellow product quality control obtained precisely, by color difference meter joint product Pureness control, can will produce
The strengths and weaknesses analysis of product it is more thorough, shows that purity is high does not represent that tone is bright-coloured, this is to terminal visitor during present invention test
For the use at family, there is considerable directive significance.
Specific embodiment
Embodiment 1
A kind of method separating different tone Gardenia Yellows from Gardenia Yellow dry fruit of the present embodiment, comprising the following steps:
(1) it is crushed: weighing Gardenia Yellow dry fruit 100kg, be crushed to selection by winnowing decladding after 2mm；
(2) it extracts: the Gardenia Yellow after decladding is used into flooding 3 times that temperature is 42 DEG C, the leaching process solid-liquid ratio
Respectively 1:8,1:5,1:3 extract 1.5h every time, after the completion of extraction, merge each leaching liquor；
(3) it is centrifuged: leaching liquor being centrifuged using butterfly centrifugal machine, flux 320L/h, clear liquid is using aperture after centrifugation
0.2 μm of microfiltration of ceramic membrane collects filtrate；
(4) it elutes: by filtrate with the absorption of PRP chromatographic resin, being eluted using linear gradient elution method, first with mass fraction for 10%
Ethanol water elution, elution volume 4BV, flow velocity 1BV, collect eluent A；The ethyl alcohol for being again 20% with mass fraction
Aqueous solution elution, elution volume 4BV, flow velocity 1BV collect eluent B；Then the ethyl alcohol for being 30% with mass fraction is water-soluble
Liquid elution, elution volume 3BV, flow velocity 0.5BV collect eluent C；Then the ethanol water for being 40% with mass fraction
Elution, elution volume 3BV, flow velocity 1BV collect eluent D；Then it is eluted with the ethanol water that mass fraction is 50%,
Elution volume is 3BV, flow velocity 1BV, collects eluent E；Then it is eluted with the ethanol water that mass fraction is 60%, elution
Volume is 2BV, flow velocity 0.5BV, collects eluent F；Then it is eluted with the ethanol water that mass fraction is 70%, elutes body
Product is 2BV, flow velocity 0.5BV, collects eluent G；Then it is eluted with the ethanol water that mass fraction is 80%, elution volume
For 2BV, flow velocity 0.5BV, eluent H is collected；
(5) it is concentrated: outer circulation type single effect evaporator is respectively adopted in above-mentioned eluent A, B, C, D, E, F, G, H and is concentrated under reduced pressure
The concentrate for being 38% to solid content, ethyl alcohol recycling, after concentrate is spray-dried, can respectively obtain Gardenia Yellow product
A, the color value of B, C, D, E, F, G, H, the Gardenia Yellow product A are 102.8, and the color value of Gardenia Yellow product B is
131.5, the color value of Gardenia Yellow products C is 357.2, and the color value of Gardenia Yellow product D is 398.8, and Gardenia Yellow produces
The color value of product E is 471.6, and the color value of Gardenia Yellow product F is 483.4, and the color value of Gardenia Yellow product G is 515.3, Cape jasmine
The color value of sub- uranidin product H is 524.8.
Eight kinds of Gardenia Yellow product A, B, C, D, E, F, G, H yields that the present embodiment obtains are respectively 7.3%, 10.6%,
21.4%, 25.8%, 14.6%, 6.2%, 7.4%, 4.1%, total recovery 97.4%.
Eight kinds of Gardenia Yellow product powder tone differences that the present embodiment obtains are obvious, see the table below 1.
1 Gardenia Yellow product powder color difference of table
| Title | L* | a* | b* | dL* | da* | db* | dE* | Y Transmission |
| Product A | 57.42 | 55.44 | 97.54 | 57.42 | 55.44 | 97.54 | 25.36 | |
| Product B | 64.77 | 45.22 | 109.02 | 7.35 | -10.21 | 11.48 | 17.03 | 33.76 |
| Products C | 66.54 | 42.70 | 111.87 | 9.11 | -12.73 | 14.32 | 21.22 | 36.02 |
| Product D | 59.15 | 33.62 | 97.40 | 1.73 | -21.82 | -0.15 | 21.89 | 27.20 |
| Product E | 68.04 | 40.54 | 113.99 | 10.62 | -14.90 | 16.44 | 24.60 | 38.03 |
| Product F | 69.20 | 38.98 | 115.84 | 11.78 | -16.55 | 18.30 | 27.34 | 39.63 |
| Product G | 73.65 | 32.55 | 122.38 | 16.23 | -22.88 | 24.83 | 37.46 | 46.16 |
| Product H | 77.02 | 27.22 | 127.30 | 19.59 | -28.22 | 29.76 | 45.45 | 51.56 |
Under normal conditions, the difference between dE* value is can determine that less than 4 as no tone difference, and the dE* in table 1 is to produce
Product A is the value of chromatism of control, and the mutual value of chromatism of product is judged with the difference of the dE* value of two kinds of products in table 1.From
The difference of dE* can be seen that eight kinds of products and have differences between each other in upper table 1, and the disparity range of dE* value is 0.67-
Between 28.42.The powder unification of eight kinds of products is diluted to E440nm=1 with pure water, the tone of aqueous solution equally exists difference
Different, for the disparity range of dE* value between 0.47-61.13, the aqueous solution color difference of each product see the table below 2.
2 Gardenia Yellow product aqueous solution color difference of table
| Title | L* | a* | b* | dL* | da* | Db* | dE* | Y Transmission |
| Product A | 95.77 | -8.80 | 23.82 | 95.77 | -8.80 | 23.82 | 89.44 | |
| Product B | 95.71 | -8.99 | 25.03 | -0.06 | -0.19 | 1.21 | 1.23 | 89.30 |
| Products C | 94.57 | -12.79 | 42.22 | -1.19 | -3.99 | 18.40 | 18.86 | 86.61 |
| Product D | 93.47 | -11.43 | 42.83 | -2.29 | -2.64 | 19.01 | 19.33 | 84.05 |
| Product E | 90.12 | -11.30 | 68.43 | -5.64 | -2.50 | 44.61 | 45.03 | 76.57 |
| Product F | 92.54 | -14.55 | 72.69 | -3.23 | -5.75 | 48.87 | 49.31 | 81.92 |
| Product G | 86.98 | -7.79 | 81.76 | -8.79 | 1.01 | 57.94 | 58.62 | 69.96 |
| Product H | 90.93 | -12.25 | 85.90 | -4.84 | -3.45 | 62.08 | 62.36 | 78.32 |
Embodiment 2
A kind of method separating different tone Gardenia Yellows from Gardenia Yellow dry fruit of the present embodiment, comprising the following steps:
(1) it is crushed: weighing Gardenia Yellow dry fruit 100kg, be crushed to selection by winnowing decladding after 1mm；
(2) it extracts: the Gardenia Yellow after decladding is used into flooding 3 times that temperature is 30 DEG C, the leaching process solid-liquid ratio
Respectively 1:8,1:5,1:3 extract 2h every time, after the completion of extraction, merge each leaching liquor；
(3) it is centrifuged: leaching liquor being centrifuged using butterfly centrifugal machine, flux 400L/h, clear liquid is using aperture after centrifugation
0.2 μm of microfiltration of ceramic membrane collects filtrate；
(4) it elutes: by filtrate with the absorption of PRP chromatographic resin, being eluted using linear gradient elution method, first with mass fraction for 10%
Ethanol water elution, elution volume 3BV, flow velocity 0.5BV, collect eluent A；The second for being again 20% with mass fraction
Alcohol solution elution, elution volume 3BV, flow velocity 0.5BV collect eluent B；Then the ethyl alcohol for being 30% with mass fraction
Aqueous solution elution, elution volume 4BV, flow velocity 1BV collect eluent C；Then the ethyl alcohol for being 40% with mass fraction is water-soluble
Liquid elution, elution volume 2BV, flow velocity 0.5BV collect eluent D；Then the ethanol water for being 50% with mass fraction
Elution, elution volume 2BV, flow velocity 0.5BV collect eluent E；Then the ethanol aqueous wash for being 60% with mass fraction
De-, elution volume 3BV, flow velocity 1BV collect eluent F；Then it is eluted, is washed with the ethanol water that mass fraction is 70%
Lift-off product is 3BV, flow velocity 1BV, collects eluent G；Then it is eluted with the ethanol water that mass fraction is 80%, elutes body
Product is 2BV, flow velocity 0.7BV, collects eluent H；
(5) it is concentrated: outer circulation type single effect evaporator is respectively adopted in above-mentioned eluent A, B, C, D, E, F, G, H and is concentrated under reduced pressure
The concentrate for being 39% to solid content, ethyl alcohol recycling, after concentrate is spray-dried, can respectively obtain Gardenia Yellow product
A, the color value of B, C, D, E, F, G, H, the Gardenia Yellow product A are 103.6, and the color value of Gardenia Yellow product B is
130.9, the color value of Gardenia Yellow products C is 355.6, and the color value of Gardenia Yellow product D is 391.5, and Gardenia Yellow produces
The color value of product E is 470.1, and the color value of Gardenia Yellow product F is 480.4, and the color value of Gardenia Yellow product G is 512.8, Cape jasmine
The color value of sub- uranidin product H is 520.7.
Eight kinds of Gardenia Yellow product A, B, C, D, E, F, G, H yields that the present embodiment obtains are respectively 7.1%, 10.2%,
21.2%, 25.3%, 14.3%, 6.4%, 7.1%, 4.5%, total recovery 96.1%, eight kinds of Gardenia Yellow product powder tone differences
Obviously, powder unification is diluted to E440nm=1 with pure water, the differentiation trend as powder is presented in tone.
Embodiment 3
A kind of method separating different tone Gardenia Yellows from Gardenia Yellow dry fruit of the present embodiment, comprising the following steps:
(1) it is crushed: weighing Gardenia Yellow dry fruit 100kg, be crushed to selection by winnowing decladding after 3mm；
(2) it extracts: the Gardenia Yellow after decladding is used into flooding 3 times that temperature is 50 DEG C, the leaching process solid-liquid ratio
Respectively 1:8,1:5,1:3 extract 1h every time, after the completion of extraction, merge each leaching liquor；
(3) it is centrifuged: leaching liquor being centrifuged using butterfly centrifugal machine, flux 200L/h, clear liquid is using aperture after centrifugation
0.2 μm of microfiltration of ceramic membrane collects filtrate；
(4) it elutes: by filtrate with the absorption of PRP chromatographic resin, being eluted using linear gradient elution method, first with mass fraction for 10%
Ethanol water elution, elution volume 3.5BV, flow velocity 0.7BV, collect eluent A；It is again 20% with mass fraction
Ethanol water elution, elution volume 3.5BV, flow velocity 0.8BV collect eluent B；It then is 30% with mass fraction
Ethanol water elution, elution volume 3.5BV, flow velocity 0.7BV collect eluent C；It then is 40% with mass fraction
Ethanol water elution, elution volume 2.6BV, flow velocity 0.7BV collect eluent D；It then is 50% with mass fraction
Ethanol water elution, elution volume 2.5BV, flow velocity 0.7BV collect eluent E；It then is 60% with mass fraction
Ethanol water elution, elution volume 2.5BV, flow velocity 0.8BV collect eluent F；It then is 70% with mass fraction
Ethanol water elution, elution volume 2.6BV, flow velocity 0.8BV collect eluent G；It then is 80% with mass fraction
Ethanol water elution, elution volume 2BV, flow velocity 1.0BV collect eluent H；
(5) it is concentrated: outer circulation type single effect evaporator is respectively adopted in above-mentioned eluent A, B, C, D, E, F, G, H and is concentrated under reduced pressure
The concentrate for being 40% to solid content, ethyl alcohol recycling, after concentrate is spray-dried, can respectively obtain Gardenia Yellow product
A, the color value of B, C, D, E, F, G, H, the Gardenia Yellow product A are 101.9, and the color value of Gardenia Yellow product B is
126.6, the color value of Gardenia Yellow products C is 351.7, and the color value of Gardenia Yellow product D is 396.4, and Gardenia Yellow produces
The color value of product E is 468.2, and the color value of Gardenia Yellow product F is 487.5, and the color value of Gardenia Yellow product G is 509.9, Cape jasmine
The color value of sub- uranidin product H is 518.6.
Eight kinds of Gardenia Yellow product A, B, C, D, E, F, G, H yields that the present embodiment obtains are respectively 7.2%, 10.8%,
20.9%, 26.1%, 13.7%, 5.9%, 6.8%, 4.1%, total recovery 95.5%, eight kinds of Gardenia Yellow product powder tone differences
Obviously, powder unification is diluted to E440nm=1 with pure water, the differentiation trend as powder is presented in tone.
Claims (6)
1. a kind of method for separating different tone Gardenia Yellows from Gardenia Yellow dry fruit, it is characterised in that the following steps are included:
(1) it is crushed: choosing Gardenia Yellow dry fruit, be crushed to selection by winnowing decladding after 1-3mm；
(2) extract: by the Gardenia Yellow after decladding using flooding 3-5 times that temperature is 30-50 DEG C, the additional amount of the water is Cape jasmine
3-8 times of sub- Huang dry fruit weight, extracts 1-2h every time, after the completion of extraction, merges each leaching liquor；
(3) it is centrifuged: leaching liquor is centrifuged, the microfiltration of ceramic membrane that clear liquid is 0.2 μm using aperture after centrifugation collects filtrate；
(4) it elutes: by above-mentioned filtrate with the absorption of PRP chromatographic resin, being eluted using linear gradient elution method, first with mass fraction for 10%
Ethanol water elution, elution volume 3-4BV, flow velocity 0.5-1BV, collect eluent A；Again with mass fraction for 20%
Ethanol water elution, elution volume 3-4BV, flow velocity 0.5-1BV, collect eluent B；Then it is with mass fraction
30% ethanol water elution, elution volume 3-4BV, flow velocity 0.5-1BV collect eluent C；Then with mass fraction
It is eluted for 40% ethanol water, elution volume 2-3BV, flow velocity 0.5-1BV collect eluent D；Then with quality point
Number elutes for 50% ethanol water, and elution volume 2-3BV, flow velocity 0.5-1BV collect eluent E；Then with quality
The ethanol water that score is 60% elutes, elution volume 2-3BV, flow velocity 0.5-1BV, collects eluent F；Then with matter
It measures the ethanol water that score is 70% to elute, elution volume 2-3BV, flow velocity 0.5-1BV collect eluent G；Then with
The ethanol water that mass fraction is 80% elutes, elution volume 2BV, flow velocity 0.5-1BV, collects eluent H；
(5) it is concentrated: above-mentioned eluent A, B, C, D, E, F, G, H is concentrated under reduced pressure into the concentrate that solid content is 30-40% respectively,
Ethyl alcohol recycling, after concentrate is spray-dried, can respectively obtain Gardenia Yellow product A, B, C, D, E, F, G, H, the Cape jasmine
The color value of sub- uranidin product A is greater than 100, and the color value of Gardenia Yellow product B is greater than 120, the color value of Gardenia Yellow products C
Greater than 340, the color value of Gardenia Yellow product D is greater than 390, and the color value of Gardenia Yellow product E is greater than 460, Gardenia Yellow
The color value of product F is greater than 480, and the color value of Gardenia Yellow product G is greater than 500, and the color value of Gardenia Yellow product H is greater than 510.
2. a kind of method for separating different tone Gardenia Yellows from Gardenia Yellow dry fruit according to claim 1, feature exist
In: the broken partial size in the step (1) is 2mm.
3. a kind of method for separating different tone Gardenia Yellows from Gardenia Yellow dry fruit according to claim 1, feature exist
In: water temperature is 40 DEG C in the step (2).
4. a kind of method for separating different tone Gardenia Yellows from Gardenia Yellow dry fruit according to claim 1, feature exist
In: it is centrifuged in the step (3) using butterfly centrifugal machine, flux 200-400L/min.
5. a kind of method for separating different tone Gardenia Yellows from Gardenia Yellow dry fruit according to claim 1, feature exist
In: it is concentrated under reduced pressure in the step (5) using outer circulation type single effect evaporator.
6. a kind of method for separating different tone Gardenia Yellows from Gardenia Yellow dry fruit according to claim 4, feature exist
In: flux is 300L/min in the step (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710632547.7A CN107286702B (en) | 2017-07-28 | 2017-07-28 | A method of separating different tone Gardenia Yellows from Gardenia Yellow dry fruit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710632547.7A CN107286702B (en) | 2017-07-28 | 2017-07-28 | A method of separating different tone Gardenia Yellows from Gardenia Yellow dry fruit |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107286702A CN107286702A (en) | 2017-10-24 |
| CN107286702B true CN107286702B (en) | 2019-03-19 |
Family
ID=60103665
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710632547.7A Active CN107286702B (en) | 2017-07-28 | 2017-07-28 | A method of separating different tone Gardenia Yellows from Gardenia Yellow dry fruit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107286702B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111574847A (en) * | 2020-04-30 | 2020-08-25 | 广州正明生物科技有限公司 | Preparation method of gardenia yellow pigment with natural antioxidant capacity |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100537586C (en) * | 2006-02-23 | 2009-09-09 | 江南大学 | Method for producing high purity jasminoidin and high color number gardenia yellow pigment |
| CN101757185A (en) * | 2008-12-11 | 2010-06-30 | 湖州来色生物基因工程有限公司 | Extraction of novel natural pigment and preparation method of inclusion compound thereof |
| CN101585757B (en) * | 2009-06-03 | 2010-06-02 | 河北食品添加剂有限公司 | Method for preparing curcumin, demethoxycurcumin and bisdemethoxycurcumin |
-
2017
- 2017-07-28 CN CN201710632547.7A patent/CN107286702B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN107286702A (en) | 2017-10-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108653417B (en) | Method for extracting sorbus nigromaculata and extract thereof | |
| CN107969532B (en) | Fructus momordicae flavored instant drink and preparation method thereof | |
| CN108299852B (en) | The technique of grading extraction cacao color from cocoa pod shells | |
| CN106333206B (en) | A kind of production method of honey taste arhat juice | |
| CN102687838A (en) | Cocoa extract preparation method | |
| CN107338280A (en) | A kind of high activity low sugar base ginseng saponin group and its preparation method of aglycon | |
| CN105482957A (en) | Rhizoma polygonati extract, rhizoma polygonati wine thereof and rhizoma polygonati wine preparation method | |
| CN105580932B (en) | Method for preparing tea leaf extract with different tastes | |
| CN104194996B (en) | A kind of Hylocereus undatus mulberry fruit wine and preparation method thereof | |
| CN107286702B (en) | A method of separating different tone Gardenia Yellows from Gardenia Yellow dry fruit | |
| CN103880806A (en) | Method for extracting anthocyanin from blueberry | |
| CN114223760A (en) | High-theaflavin instant black tea powder and in-vitro oxidation preparation method and application thereof | |
| CN108359571A (en) | A kind of production technology of white tea fruit Spirit | |
| CN106721844A (en) | A kind of preparation method of matrimony vine Momordica grosvenori electuary | |
| CN105199900A (en) | Purple sweet potato wine and preparation method thereof | |
| CN106046846A (en) | Method for extracting peel pigment from green plums | |
| CN105231355A (en) | Lotus root instant powder | |
| CN102342341B (en) | Standard quantitatively-blended instant oolong tea and preparation method thereof | |
| CN109527150A (en) | A kind of preparation method of high-quality instant black tea powder | |
| CN105039043A (en) | Salivation-promoting banana and musa basjoo healthcare beer and processing method thereof | |
| CN108949408A (en) | A kind of preparation method of functional form Chinese-wolfberry nutritive yellow rice wine | |
| CN105462752A (en) | Phellinus igniarius and folium mori rice wine and preparation method thereof | |
| CN111418687A (en) | Preparation method of coffee-flavored solid beverage | |
| KR102464806B1 (en) | Method for producing red ginseng thin section using ginseng or red ginseng | |
| CN114377039B (en) | Extraction and separation method of ganoderma lucidum multicomponent and application of ganoderma lucidum multicomponent in preparation of artificially planted ganoderma lucidum extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |