CN103880806A - Method for extracting anthocyanin from blueberry - Google Patents
Method for extracting anthocyanin from blueberry Download PDFInfo
- Publication number
- CN103880806A CN103880806A CN201410147398.1A CN201410147398A CN103880806A CN 103880806 A CN103880806 A CN 103880806A CN 201410147398 A CN201410147398 A CN 201410147398A CN 103880806 A CN103880806 A CN 103880806A
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- China
- Prior art keywords
- blueberry
- raw material
- ethanol
- alcohol
- filtrate
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for extracting anthocyanin from blueberry. The method comprises (1) generation of pigment powder: A, leaching, including the step of extracting fresh blueberry fruits for 24 hours by use of 30% ethanol under a condition that the mass ratio of a raw material to leaching liquid is 1:2, B, concentration, including the step of rotationally evaporating at the temperature of 50 DEG C to remove the ethanol, C, chromatography, including the step of eluting by use of 95% of ethanol, D, extraction, including the step of extracting eluant by use of petroleum ether of which the volume is three times that of concentrated liquid and E, drying; (2) production of pigment extract: A, leaching, including the step of adding 3L of 85% ethanol into 1kg of raw material, B, squeezing, including the step of removing fruit dregs to obtain filtrate, C, cooling and filtration and D, concentrating to obtain blueberry pigment extract. The method disclosed by the invention is simple in process and has the effects of greatly increasing the yield, purity and color value of blueberry anthocyanin.
Description
Technical field
The present invention relates to a kind of method of extracting anthocyanidin from blueberry.
Background technology
Although China's blueberry resource is extremely abundant, but because the common people are for the many factors such as not high, the existing processing technology level of cognition degree of blueberry resource is low, blueberry resource does not obtain effective exploitation utilization, most of blueberry resource is all the form direct export with raw material, added value of product is not high, and economical, societal benefits are poor; The product of blueberry has Normal juice, fermented juice, inspissated juice, fruit wine, jam, jelly, fruit, candy, sugaring goods, quick-frozen fruit etc., processing means falls behind, the nutritive value of product and absorption rate are not very high, utilize the quantity of the high-end products such as blueberry resource production vaccinium oxycoccus pigment also little.Aspect product deep processing, focus mostly in the exploitation of the low value-added products such as instant blueberry and blueberry juice, blueberry sauce, because extracting problem, vaccinium oxycoccus pigment effectively solved all the time, the comprehensive utilization of blueberry is limited to, and therefore the intensive processing of vaccinium oxycoccus pigment and industrialization are rather urgently.
Summary of the invention
The object of the invention is to overcome the deficiency that above-mentioned prior art exists, and a kind of method of extracting anthocyanidin from blueberry is provided.
The technical scheme adopting is
From blueberry, extract a method for anthocyanidin, comprise following technological process:
(1) production of pigmented powder
A. lixiviate
Blueberry fresh fruit 30% extraction using alcohol, raw material and vat liquor ratio are 1:2(mass ratio), alcohol extracting 24 hours.Filter to obtain liquid (alcohol residue 9%) through sieve, method residue alcohol extracting 2 times more according to this, three times filtrate is mixed.
B. concentrated
50 DEG C of rotary evaporations are removed ethanol.
C. chromatography
Concentrated solution, through AB-8 macroporous adsorbent resin, is used 95% ethanol elution.
D. extraction
The petroleum ether extraction of three times of concentrated solution volumes for elutriant, takes off a layer liquid.
E. dry
Through vacuum freeze drier, negative 100 normal atmosphere, trap 40 degrees below zero left and right, under dividing plate 60 degree left and right, dry Dry Sack cellulose crystal.
(2) production of pigment medicinal extract
A. lixiviate
Blueberry fresh fruit 85% extraction using alcohol, raw material and vat liquor ratio are that 1 part of water-containing material (quality) adds 3 portions of (capacity) vat liquors, 1 ㎏ raw material adds 3L 85% ethanol.Owing to containing moisture in raw material, vat liquor alcohol concn reality 65%, mixes raw material and ethanol, is heated to 50-60 DEG C, lixiviate 4 hours, and stir at any time.
B. squeezing
By extractive substance squeeze and filter, remove pomace, obtain filtrate.
C. cooling, filtration
Filtrate is cooled to after room temperature (15-20 DEG C), and fine filtering or centrifugal, except throw outs such as desaccharification, pectin, obtains filtrate.Throw out is used a small amount of 65% washing with alcohol 1 time again, and merging filtrate is centrifugal.
D. concentrated
Heating is concentrated, makes the water content of concentrated solution lower than 30%, obtains blueberry pigment medicinal extract.
The present invention utilizes ultrasonic wave double solvents extractive technique to be applied in Anthocyanin from Blueberry extraction process, can extract to greatest extent blueberry pigment, the physiologically active of protection Anthocyanin from Blueberry.Meanwhile, utilize chromatographic technique, chromatographic separation technology, the ultra-filtration technique of double solvents gradient elution to remove impurity, technique is simple, can greatly improve yield, purity and the look valency of vaccinium oxycoccus pigment.
Embodiment
A kind of method of extracting anthocyanidin from blueberry of the present invention, comprises following technological process:
(1) production of pigmented powder
A. lixiviate
Blueberry fresh fruit 30% extraction using alcohol, raw material and vat liquor ratio are 1:2(mass ratio), alcohol extracting 24 hours.Filter to obtain liquid (alcohol residue 9%) through sieve, method residue alcohol extracting 2 times more according to this, three times filtrate is mixed.
B. concentrated
50 DEG C of rotary evaporations are removed ethanol.
C. chromatography
Concentrated solution, through AB-8 macroporous adsorbent resin, is used 95% ethanol elution.
D. extraction
The petroleum ether extraction of three times of concentrated solution volumes for elutriant, takes off a layer liquid.
E. dry
Through vacuum freeze drier, negative 100 normal atmosphere, trap 40 degrees below zero, under dividing plate 60 degree, dry Dry Sack cellulose crystal.
(2) production of pigment medicinal extract
A. lixiviate
Blueberry fresh fruit 85% extraction using alcohol, raw material and vat liquor ratio are that 1 part of water-containing material taking quality as unit adds
3 portions of vat liquors taking capacity as unit, 1 ㎏ raw material adds 3L 85% ethanol, and owing to containing moisture in raw material, vat liquor alcohol concn reality 65%, mixes raw material and ethanol, is heated to 60 DEG C, lixiviate 4 hours, and stir at any time;
B. squeezing;
By extractive substance squeeze and filter, remove pomace, obtain filtrate;
C. cooling, filtration;
Filtrate is cooled to after room temperature 16, and fine filtering or centrifugal, except throw outs such as desaccharification, pectin, obtains filtrate.Throw out is used a small amount of 65% washing with alcohol 1 time again, and merging filtrate is centrifugal;
D. concentrated
Heating is concentrated, makes the water content of concentrated solution lower than 30%, obtains blueberry pigment medicinal extract.
Claims (1)
1. a method of extracting anthocyanidin from blueberry, its feature comprises following technological process:
(1) production of pigmented powder
A. lixiviate
Blueberry fresh fruit 30% extraction using alcohol, raw material and vat liquor in mass ratio example are 1:2, alcohol extracting 24 hours, filters to obtain liquid through sieve, method residue alcohol extracting 2 times more according to this, three times filtrate is mixed;
B. concentrated
50 DEG C of rotary evaporations are removed ethanol;
C. chromatography
Concentrated solution, through AB-8 macroporous adsorbent resin, is used 95% ethanol elution;
D. extraction
The petroleum ether extraction of three times of concentrated solution volumes for elutriant, takes off a layer liquid;
E. dry
Through vacuum freeze drier, negative 100 normal atmosphere, trap 40 degrees below zero, under dividing plate 60 degree, dry Dry Sack cellulose crystal;
(2) production of pigment medicinal extract
A. lixiviate
Blueberry fresh fruit 85% extraction using alcohol, raw material and vat liquor ratio are that 1 part of water-containing material taking quality as unit adds 3 portions of vat liquors taking capacity as unit, 1 ㎏ raw material adds 3L 85% ethanol, owing to containing moisture in raw material, vat liquor alcohol concn reality 65%, mixes raw material and ethanol, is heated to 50-60 DEG C, lixiviate 4 hours, and stir at any time;
B. squeezing;
By extractive substance squeeze and filter, remove pomace, obtain filtrate;
C. cooling, filtration;
Filtrate is cooled to after room temperature 15-20 DEG C, and fine filtering or centrifugal, except throw outs such as desaccharification, pectin, obtains filtrate;
Throw out is used a small amount of 65% washing with alcohol 1 time again, and merging filtrate is centrifugal;
D. concentrated
Heating is concentrated, makes the water content of concentrated solution lower than 30%, obtains blueberry pigment medicinal extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410147398.1A CN103880806A (en) | 2014-04-14 | 2014-04-14 | Method for extracting anthocyanin from blueberry |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410147398.1A CN103880806A (en) | 2014-04-14 | 2014-04-14 | Method for extracting anthocyanin from blueberry |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103880806A true CN103880806A (en) | 2014-06-25 |
Family
ID=50949973
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410147398.1A Pending CN103880806A (en) | 2014-04-14 | 2014-04-14 | Method for extracting anthocyanin from blueberry |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103880806A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016146882A1 (en) | 2015-03-16 | 2016-09-22 | Mika Tapio Reijonen | Method of extraction of berries or fruits using an alkaline magnesium compound for pharmaceutical, herbal, nutritional or cosmetic ingredients |
| CN107056740A (en) * | 2017-05-03 | 2017-08-18 | 赣州华汉生物科技有限公司 | A kind of method that anthocyanidin is extracted from blueberry pomace |
| CN107927702A (en) * | 2017-11-06 | 2018-04-20 | 云南省盐业有限公司 | A kind of natural seven-color nourishing salt |
| CN112545951A (en) * | 2019-09-25 | 2021-03-26 | 深圳光彩生命工程技术有限公司 | Nano-liposome antioxidant membrane containing active plant extract |
| CN114230543A (en) * | 2022-01-12 | 2022-03-25 | 万华化学集团股份有限公司 | Method for extracting anthocyanin from arabidopsis thaliana petals |
-
2014
- 2014-04-14 CN CN201410147398.1A patent/CN103880806A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016146882A1 (en) | 2015-03-16 | 2016-09-22 | Mika Tapio Reijonen | Method of extraction of berries or fruits using an alkaline magnesium compound for pharmaceutical, herbal, nutritional or cosmetic ingredients |
| CN107056740A (en) * | 2017-05-03 | 2017-08-18 | 赣州华汉生物科技有限公司 | A kind of method that anthocyanidin is extracted from blueberry pomace |
| CN107927702A (en) * | 2017-11-06 | 2018-04-20 | 云南省盐业有限公司 | A kind of natural seven-color nourishing salt |
| CN112545951A (en) * | 2019-09-25 | 2021-03-26 | 深圳光彩生命工程技术有限公司 | Nano-liposome antioxidant membrane containing active plant extract |
| CN114230543A (en) * | 2022-01-12 | 2022-03-25 | 万华化学集团股份有限公司 | Method for extracting anthocyanin from arabidopsis thaliana petals |
| CN114230543B (en) * | 2022-01-12 | 2023-12-19 | 万华化学集团股份有限公司 | Method for extracting anthocyanin from Arabidopsis petals |
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| Date | Code | Title | Description |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140625 |