CN107353356A - The extracting method of Polysaccharides of L. deliciosus - Google Patents
The extracting method of Polysaccharides of L. deliciosus Download PDFInfo
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- CN107353356A CN107353356A CN201710482209.XA CN201710482209A CN107353356A CN 107353356 A CN107353356 A CN 107353356A CN 201710482209 A CN201710482209 A CN 201710482209A CN 107353356 A CN107353356 A CN 107353356A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
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Abstract
The invention discloses the extracting method of Polysaccharides of L. deliciosus, method includes the pretreatment of fructification, Steam explosion treatment, and hot water extraction takes off albumen, and alcohol precipitation is dried.Have the beneficial effect that：The water extraction Polysaccharides of L. deliciosus time is long and efficiency is low, the present invention carries out Steam explosion treatment to the delicious lactarius by pretreatment first, polysaccharide in reusable heat flooding delicious lactarius, the defects of overcoming prior art, the yield of Polysaccharides of L. deliciosus is improved, shorten the time of extraction, and extracting method rational technology, it is simple to operate, production cost is reduced, advantage is provided for industrial applications.
Description
Technical field
The present invention relates to medical processing technique field, more particularly, to the extracting method of Polysaccharides of L. deliciosus.
Background technology
Delicious lactarius, delicious lactarius category Russulaceae, Lactarius, also known as delicious delicious lactarius, pine tree mushroom, loose bacterium, fir mushroom, cogongrass
Mushroom, it is a kind of well received delicious edible mushroom.Fructification is medium to large-scale, the flat hemispherical of cap, peeled shrimp color, carrot yellow
Or darkorange, just band is white for bacterial context, rear to become carrot yellow.Amount of milk is few, and salmon pink, lamella is homochromy with cap, the nearly cylinder of stem
Shape is simultaneously tapered to base portion, the summer and autumn single life in ground or all living creatures in broad-leaf forest, forms mycorhiza.Edible.Taste is soft, and rear taste is slightly
Pungent, taste is good.Widely distributed in China, often yield is big, is easy to collect, processes, sells.Polysaccharide is important informational molecule,
Participate in a variety of vital movements of body.Numerous studies confirm that polysaccharide has various biological effect, such as antiviral, antitumor, drop blood
Sugar, reducing blood lipid and immunological regulation etc..Polysaccharide compound has wide in fields such as health care, medicine, cosmetics and food additives
General application prospect.Deep development of the extraction process to delicious lactarius resource of Polysaccharides of L. deliciosus is studied with the scientific method in modern times
There is facilitation, improve the economic benefit of agricultural byproducts.
Prior art such as Authorization Notice No. is the B of CN 102850462 Chinese invention patent, and it is more to disclose a kind of delicious lactarius
The extracting method of sugar, with hot water extraction's delicious lactarius three times, each 6h, then by separating to obtain Polysaccharides of L. deliciosus, hot water in the invention
Long the time required to extraction, recovery rate is low, causes the waste of delicious lactarius resource, adds the cost of technique.
The content of the invention
It is an object of the invention to provide a kind of extracting method of Polysaccharides of L. deliciosus, the method overcome prior art extraction
The shortcomings that rate is low, extraction time is long.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：The extracting method of Polysaccharides of L. deliciosus,
Pretreatment including fructification, Steam explosion treatment, hot water extraction take off albumen, and alcohol precipitation is dried.Method is reasonable, simple to operate, carries
Take rate high, the Polysaccharides of L. deliciosus activity obtained is good, reduces production cost, and advantage is provided for industrial applications.
Preferably, in the preprocessing process of fructification, take delicious lactarius fructification to clean, 30- is dried at 60-65 DEG C
40min, crush, cross 40-45 mesh sieves, obtain delicious lactarius powder.Delicious lactarius passes through pulverization process, improves it in steam blasting process
In contact area with water vapour, be beneficial to infiltration of the steam to cell membrane, so as to improve the shell-broken effect of steam.
Preferably, during Steam explosion treatment, 5-7 part delicious lactarius powder is taken, adds distilled water, adjustment ratio of water to material is
12-15%, 30-35min is stirred, sealing and standing 7-9h, obtains prepreg solution, 3-7 part bioactive peptides, stirring are added into prepreg solution
10-16min, then Steam explosion treatment is carried out, Stress control 2-2.6MPa, the dimension pressure time is 35-43s, obtains explosion liquid.To pine
Newborn mushroom carries out Steam explosion treatment, can greatly promote the rupture of cell membrane and the release of polysaccharide, reduce the resistance of hot water extraction,
Reduce the time of extraction, improve the recovery rate of Polysaccharides of L. deliciosus.
Preferably, the amino acid sequence of the bioactive peptide added during Steam explosion treatment is：
HSHACASYKLCVCVNCSR.During steam blasting, the steam penetrated into the space of cell membrane is limited, causes broken wall degree
It is not high, the addition of bioactive peptide, the infiltration of steam is promoted, improves shell-broken effect, significantly reduce the resistance of hot water extraction
Power, greatly shorten extraction time.
Preferably, during hot water extraction, 6-10 part water is added into explosion liquid, 40- is extracted at 92-99 DEG C
51min, 10-14min is centrifuged in the case where rotating speed is 8000-10000r/min, supernatant is taken, obtains leaching liquor.Using water as extraction
Polysaccharide, safety and environmental protection, cost is cheap, because Steam explosion treatment has abolished delicious lactarius cell membrane, greatly releases intracellular
Polysaccharide, in a short time can be to reach good extracting effect.
Preferably, in de- protein process, chloroform and n-butanol are configured to mixed liquor, the wherein chloromethane of volume ratio three
Alkane/n-butanol=3.3/1-3.5/1,1-3 part leaching liquors are taken, add the mixed liquor that 14-18 parts are configured thereto, stir 32-
40min, centrifuge, phase of fetching water.Solution obtained by hot water extraction's process contains many free proteins, drops the purity of polysaccharide
Low, after adding the mixed liquor of chloroform and n-butanol, protein can form precipitation and be separated out from solution, improve the pure of polysaccharide
Degree, strengthen the bioactivity of product.
Preferably, in alcohol precipitation drying process, 22-26 parts 86%-93% second is added in the aqueous phase obtained to de- albumen
Alcoholic solution, 16-26min, filtering are stirred, setting vacuum is 3000-5000Pa, and temperature is 62-70 DEG C, by filter residue and drying 25-
36min obtains polyoses extract.Polysaccharide is dissolved in hot water and the ethanol insoluble in high concentration, after 86%-93% ethanol solution is added,
Polysaccharides of L. deliciosus is separated out in the form of precipitating from aqueous phase, and the Polysaccharides of L. deliciosus purity of gained is high, quality better, improves product
Economic value.
Compared with prior art, the advantage of the invention is that：The water extraction Polysaccharides of L. deliciosus time is long and efficiency is low, the present invention
Steam explosion treatment is carried out to the delicious lactarius by pretreatment first, has effectively abolished the cell membrane for hindering mass transport process, is promoted
Enter the release of polysaccharide, reduced the resistance to mass tranfer of hot water extraction, shortened the time of extraction, improve the receipts of Polysaccharides of L. deliciosus
Rate, plant resources is fully utilized, meet green requirement, and extracting method is reasonable, it is simple to operate, reduce
Production cost, for industrial applications provides advantage.
Embodiment
Below by embodiment, the present invention will be further described：
Embodiment 1：
The extracting method of Polysaccharides of L. deliciosus, comprises the following steps：It is the pretreatment of fructification first：Take delicious lactarius fructification clear
Wash, 40min is dried at 65 DEG C, crush, cross 45 mesh sieves, obtain delicious lactarius powder；Followed by Steam explosion treatment：Take 5 parts of pine breasts
Mushroom powder end, is put into open-top receptacle, adds distilled water, and adjustment ratio of water to material is 12%, stirs 30min, is sealed and sealed with preservative film,
7h is stood, obtains prepreg solution, 3 parts of bioactive peptides are added into prepreg solution, stirs 10min, then carries out Steam explosion treatment, pressure control
2MPa is made as, the dimension pressure time is 35s, obtains explosion liquid.Steam explosion treatment is carried out to delicious lactarius, the broken of cell membrane can be greatly promoted
The release with polysaccharide is split, reduces the resistance of hot water extraction, reduces the time of extraction, improves the extraction of Polysaccharides of L. deliciosus
Rate；Followed by hot water extraction：6 parts of water are added into explosion liquid, it is 92 DEG C, stirring and leaching 40min to set water-bath pot temperature, is being turned
Speed is to centrifuge 10min under 8000r/min, takes supernatant, obtains leaching liquor.Using water as extraction, safety economy, and due to steam
Explosion treatment greatly releases intracellular polysaccharide, in a short time can be to reach good extracting effect；Followed by de-
Albumen：Chloroform and n-butanol are configured to mixed liquor, wherein volume ratio chloroform/n-butanol=3.3/1, take 1 part of extraction
Liquid, 14 portions of mixed liquors configured are added thereto, stir 32min, centrifuge, phase of fetching water.Obtained by hot water extraction's process
Solution contains many free proteins, makes the purity of polysaccharide reduce, after adding the mixed liquor of chloroform and n-butanol, protein
Precipitation can be formed to separate out from solution, the purity of polysaccharide is improved, strengthen the bioactivity of product；It is finally that alcohol precipitation is dried：To de-
22 part 86% of ethanol solution is added in the aqueous phase that albumen is obtained, stirs 16min, precipitation is collected by filtration, setting vacuum is
3000Pa, temperature are 62 DEG C, and filter residue and drying 25min is obtained into polyoses extract.After the ethanol solution of high concentration is added, delicious lactarius
Polysaccharide forms precipitation, realizes the separation of product.
Embodiment 2：
The extracting method of Polysaccharides of L. deliciosus, comprises the following steps：
1) preprocessing process of fructification：Take delicious lactarius fructification to clean, 30min dried at 60 DEG C, crush, cross 40 mesh sieves,
Obtain delicious lactarius powder.Delicious lactarius passes through pulverization process, improves its contact area during steam blasting with water vapour, has
Beneficial to infiltration of the steam to cell membrane, so as to improve the shell-broken effect of steam；
2) Steam explosion treatment：7 parts of delicious lactarius powder are taken, add distilled water, adjustment ratio of water to material is 14%, stirs 34min, sealing
8h is stood, obtains prepreg solution, 6 parts of bioactive peptides are added into prepreg solution, stirs 15min, then carries out Steam explosion treatment, pressure control
2.5MPa is made as, the dimension pressure time is 42s, obtains explosion liquid.Steam explosion treatment is carried out to delicious lactarius, cell membrane can be greatly promoted
Rupture and the release of polysaccharide, reduce the resistance of hot water extraction, reduce the time of extraction, improve the extraction of Polysaccharides of L. deliciosus
Rate；
3) hot water extraction：9 parts of water are added into explosion liquid, 50min is extracted at 98 DEG C, are centrifuged in the case where rotating speed is 10000r/min
13min, supernatant is taken, obtain leaching liquor.Using water as extraction polysaccharide, safety and environmental protection, cost is cheap, due to Steam explosion treatment
Delicious lactarius cell membrane has been abolished, has greatly released intracellular polysaccharide, can have been imitated in a short time with reaching extraction well
Fruit；
4) albumen is taken off：Chloroform and n-butanol are configured to mixed liquor, wherein volume ratio chloroform/n-butanol=3.5/1,
3 portions of leaching liquors are taken, add 18 portions of mixed liquors configured thereto, stir 40min, are centrifuged, phase of fetching water.Hot water extraction's mistake
Solution obtained by journey contains many free proteins, makes the purity of polysaccharide reduce, and adds the mixed liquor of chloroform and n-butanol
Afterwards, protein can form precipitation and be separated out from solution, improve the purity of polysaccharide, strengthen the bioactivity of product；
5) alcohol precipitation is dried：25 part 92% of ethanol solution is added in the aqueous phase obtained to de- albumen, stirs 25min, is filtered, if
It is 5000Pa to put vacuum, and temperature is 70 DEG C, and filter residue and drying 35min is obtained into polyoses extract.Polysaccharide is dissolved in hot water and not dissolved in
The ethanol of high concentration, after ethanol solution is added, Polysaccharides of L. deliciosus is separated out in the form of precipitating from aqueous phase, the delicious lactarius of gained
Purity of polysaccharide is high, quality better, improves the economic value of product.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Lanxi City silence bio tech ltd
<120>The extracting method of Polysaccharides of L. deliciosus
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 18
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Ala Cys Ala Ser Tyr Lys Leu Cys Val Cys Val Asn Cys
1 5 10 15
Ser Arg
Claims (8)
1. the extracting method of Polysaccharides of L. deliciosus, include the pretreatment of fructification, Steam explosion treatment, hot water extraction, take off albumen, alcohol
Heavy drying, it is characterised in that：The Steam explosion treatment process is：5-7 part delicious lactarius powder is taken, adds distilled water, adjusts water
Expect that ratio is 12-15%, stir 30-35min, sealing and standing 7-9h, obtain prepreg solution, 3-7 part bioactive peptides are added into prepreg solution,
10-16min is stirred, then carries out Steam explosion treatment, Stress control 2-2.6MPa, the dimension pressure time is 35-43s, obtains explosion liquid.
2. the extracting method of Polysaccharides of L. deliciosus according to claim 1, it is characterised in that：The amino of described bioactive peptide
Acid sequence is：HSHACASYKLCVCVNCSR.
3. the extracting method of Polysaccharides of L. deliciosus according to claim 1, it is characterised in that：Described hot water extraction's process
For：6-10 part water is added into explosion liquid, 40-51min is extracted at 92-99 DEG C, centrifuges to obtain leaching liquor.
4. the extracting method of Polysaccharides of L. deliciosus according to claim 3, it is characterised in that：In described centrifugal separation processes
Rotating speed is 8000-10000r/min, time 10-14min.
5. the extracting method of Polysaccharides of L. deliciosus according to claim 1, it is characterised in that：The pretreatment of described fructification
Process is：Take delicious lactarius fructification to clean, 30-40min is dried at 60-65 DEG C, crush, cross 40-45 mesh sieves, obtain delicious lactarius powder
End.
6. the extracting method of Polysaccharides of L. deliciosus according to claim 1, it is characterised in that：Described de- protein process is：
Chloroform and n-butanol are configured to mixed liquor, wherein volume ratio chloroform/n-butanol=3.3/1-3.5/1, take 1-3 parts to soak
Extract, the mixed liquor that 14-18 parts are configured is added thereto, stir 32-40min, centrifuge, phase of fetching water.
7. the extracting method of Polysaccharides of L. deliciosus according to claim 1, it is characterised in that：Described alcohol precipitation drying process
For：22-26 parts 86%-93% ethanol solution is added into the aqueous phase obtained by de- albumen, stirs 16-26min, filtering, by filter residue
It is dried in vacuo to obtain polyoses extract.
8. the extracting method of Polysaccharides of L. deliciosus according to claim 7, it is characterised in that：In described alcohol precipitation drying process
Vacuum drying condition is：Vacuum is 3000-5000Pa, and temperature is 62-70 DEG C, time 25-36min.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108341892A (en) * | 2018-05-09 | 2018-07-31 | 四川岚晟生物科技有限公司 | A kind of preparation method of Inonotue obliquus |
| CN110357982A (en) * | 2019-07-25 | 2019-10-22 | 芜湖职业技术学院 | A method of extracting Polysaccharide in Pleurotus eryngii |
| CN113185393A (en) * | 2021-04-30 | 2021-07-30 | 中南林业科技大学 | Guaiane type sesquiterpene compound, preparation method and application thereof |
| CN115975068A (en) * | 2022-12-26 | 2023-04-18 | 上海市农业科学院 | Velvet antler mushroom polysaccharide rich in beta-glucan and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010018607A (en) * | 2008-06-11 | 2010-01-28 | Univ Of Ryukyus | Physiologically active polysaccharide and use thereof |
| CN102268467A (en) * | 2010-06-02 | 2011-12-07 | 湖南民康生物技术研究所 | Polysaccharide extract of Lactarius deliciosus hypha and application thereof |
| CN102824377A (en) * | 2012-09-20 | 2012-12-19 | 江南大学 | Method for extracting functional ingredients from lucid ganoderma sporocarp |
| CN102850462A (en) * | 2012-03-23 | 2013-01-02 | 西华师范大学 | Lactarius deliciosus polysaccharide and application thereof |
| CN103626886A (en) * | 2013-11-20 | 2014-03-12 | 中国农业科学院农产品加工研究所 | Method for extracting cereal seed coat active polysaccharide through assisted treatment of hot-pressing steam spray explosion method |
-
2017
- 2017-06-22 CN CN201710482209.XA patent/CN107353356A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010018607A (en) * | 2008-06-11 | 2010-01-28 | Univ Of Ryukyus | Physiologically active polysaccharide and use thereof |
| CN102268467A (en) * | 2010-06-02 | 2011-12-07 | 湖南民康生物技术研究所 | Polysaccharide extract of Lactarius deliciosus hypha and application thereof |
| CN102850462A (en) * | 2012-03-23 | 2013-01-02 | 西华师范大学 | Lactarius deliciosus polysaccharide and application thereof |
| CN102824377A (en) * | 2012-09-20 | 2012-12-19 | 江南大学 | Method for extracting functional ingredients from lucid ganoderma sporocarp |
| CN103626886A (en) * | 2013-11-20 | 2014-03-12 | 中国农业科学院农产品加工研究所 | Method for extracting cereal seed coat active polysaccharide through assisted treatment of hot-pressing steam spray explosion method |
Non-Patent Citations (1)
| Title |
|---|
| 敖常伟 等: "松乳菇营养成分分析及松乳菇多糖的提取分离", 《食品工业科技》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108341892A (en) * | 2018-05-09 | 2018-07-31 | 四川岚晟生物科技有限公司 | A kind of preparation method of Inonotue obliquus |
| CN110357982A (en) * | 2019-07-25 | 2019-10-22 | 芜湖职业技术学院 | A method of extracting Polysaccharide in Pleurotus eryngii |
| CN113185393A (en) * | 2021-04-30 | 2021-07-30 | 中南林业科技大学 | Guaiane type sesquiterpene compound, preparation method and application thereof |
| CN115975068A (en) * | 2022-12-26 | 2023-04-18 | 上海市农业科学院 | Velvet antler mushroom polysaccharide rich in beta-glucan and preparation method thereof |
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