CN109644776A - Method is grown in the auxiliary cordyceps sinensis training for educating combination of a kind of artificial propagation and field - Google Patents

Method is grown in the auxiliary cordyceps sinensis training for educating combination of a kind of artificial propagation and field Download PDF

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Publication number
CN109644776A
CN109644776A CN201910037806.0A CN201910037806A CN109644776A CN 109644776 A CN109644776 A CN 109644776A CN 201910037806 A CN201910037806 A CN 201910037806A CN 109644776 A CN109644776 A CN 109644776A
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cordyceps sinensis
larva
auxiliary
worm
grown
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CN201910037806.0A
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王颖
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms

Abstract

The invention discloses the auxiliary cordyceps sinensis trainings for educating combination of a kind of artificial propagation and field to grow method, comprising steps of step 1, artificial breeding, indoors in environment, under conditions of temperature and humidity control, successively carry out worm pupa hatch, mate, adopting ovum, larva culture, Spawn incubation process, obtains of the right age larva, Chinese coat spore bacterium solution;Step 2, wild auxiliary carry out field exercise a period of time educated, of the right age larva dispensing to suitable cordyceps sinensis is grown, carry out bacterium worm with Chinese coat spore bacterium solution to infect, and carry out include it is wild it is auxiliary educate illumination, moisture, temperature controlled final-period management, timely collecting obtains cordyceps sinensis.Cordyceps sinensis training is grown and is divided into two stages, differentiation control, both it had been able to achieve the segmentation cultivation of cordyceps sinensis, divide and is completed by producer, peasants and herdsmen, realize that enterprise, peasants and herdsmen's economic benefits and social benefits increase income, while being also able to achieve the wild and artificial combination of cordyceps sinensis, while improving output, enhance its drug genuineness, promotes cordyceps sinensis quality.

Description

Method is grown in the auxiliary cordyceps sinensis training for educating combination of a kind of artificial propagation and field
Technical field
The invention belongs to cordyceps sinensis to train plantation technology field, specifically, being related to a kind of artificial propagation and field is auxiliary educates knot Method is grown in the cordyceps sinensis training of conjunction.
Background technique
Cordyceps sinensis, nickname cordyceps are the complexs of aweto and Hepialidae larva, and cordyceps sinensis effect is many It is more, it be by Hypocreales, snakelike cordyceps sinensis section, snakelike Cordyceps aweto parasitize in alpine meadow soil bat moth children Worm makes larva body ossify, and under optimum conditions, summer is gone out long rod-shaped stroma by bombys batryticatus head end pumping and formed, i.e., The complex that the fructification and bombys batryticatus sclerotium (larva corpse) of aweto are constituted.Main active is cordycepin, winter worm Summer grass effect, which mainly has, adjusts function of immune system, antitumor, antifatigue, invigorating the lung and the kidney, hemostasis and phlegm, secret lean gas, whitening It the multiple efficacies such as dispels black.Currently, cordyceps sinensis artificial propagation techniques popularization be used widely, artificial propagation techniques majority be It is cultivated under conditions of indoor temperature control control is wet, though can quickly breed, disadvantage is also obvious: first is that it cannot be guaranteed that cordyceps sinensis Medicinal material genuineness;It is promoted second is that can not achieve to peasants and herdsmen, can not solve the problems, such as that peasants and herdsmen increase income, easily cause artificial propagation work Work is hindered in producing region.
In view of this present invention is specifically proposed.
Summary of the invention
The technical problem to be solved in the present invention is that overcoming the deficiencies of the prior art and provide a kind of artificial propagation and field is auxiliary Method is grown in the cordyceps sinensis training for educating combination, in order to solve the above technical problems, the present invention is using the basic conception of technical solution:
Method is grown in the auxiliary cordyceps sinensis training for educating combination of a kind of artificial propagation and field, comprising the following steps:
Step 1, artificial breeding indoors in environment, under conditions of temperature and humidity control, successively carries out worm pupa and hatches, mates, adopts Ovum, larva culture, Spawn incubation process obtain of the right age larva, Chinese coat spore bacterium solution;
Step 2, it is wild it is auxiliary educate, when of the right age larva to be launched to one section of the exercise of carry out field grow to suitable cordyceps sinensis Between, with Chinese coat spore bacterium solution carry out bacterium worm infect, and carry out include it is wild it is auxiliary educate illumination, moisture, the temperature controlled later period pipe Reason, timely collecting obtain cordyceps sinensis.
Further, in the step 1,
The hatching of worm pupa: temperature control is at 20-25 DEG C, and humid control is in 45-55%, and hatching is at moth within 20-30 days;
Mating: epidemic disaster remains unchanged, and keeps quite, and avoids noise effect, completes to hand in fly net enclosed environment Match;
It adopts ovum: in worm moth mating place, adding 1% urea solution to attract worm moth natural spawning with soft wheat bran, and receive in time Collect worm's ovum, surveys sample quantity;
Egg hatch: improving 5-8 DEG C for indoor temperature, worm's ovum be put into hatching house, is up to 80% or more to hatching rate Move out larva culture between;
Larva culture: between larva culture, temperature is adjusted to 20-25 DEG C, raises after being smashed with dedicated plant feed to 3-4 Age obtains of the right age larva;
Cultural hypha: the Chinese coat spore starter kind of purified domestication is cultivated into 3 periods, stoste in cultural hypha tank It is spare in 0-4 DEG C of refrigeration.
Further, in the step 2,
Field take exercise: carried out at 10 DEG C of natural temperature or more, band raising box soil, be placed into no mouse, without miscellaneous bacteria, without other The sand of insect pest is taken exercise, covering non-woven fabrics or stalk, and exercise time 5-10 days, wherein soil moisture 60%;
Bacterium worm is infected: Chinese coat spore bacterium solution being diluted to 0.1-0.5% concentration, imposes on larva soil surface by spraying, often It is applied at dusk, and continuous 7 days, discovery larva appearance was ossified, that is, shows to infect success;
Final-period management: after keeping soil epidemic disaster, discovery stroma to grow polypide, humidity can be suitably reduced, interval provides light According to 1 centimetre of stroma or more can harvest, and soil and coating are brushed away after harvest, obtains cordyceps sinensis.
After adopting the above technical scheme, the present invention has the advantages that compared with prior art.
The present invention, which grows cordyceps sinensis training, is divided into two stages, and by the artificial breeding stage, larva growth period environment is suitable The management of answering property solves the problems, such as to adapt to local climate difficulty;It is wild it is auxiliary educate the stage, management is infected by larva field, auxiliary educates pipe Reason, infection rate is high under indoor conditions by no means for solution, the undesirable problem of cordyceps sinensis growing environment.It is controlled using two stage differentiation System had both been able to achieve the segmentation cultivation of cordyceps sinensis, artificial breeding part had been completed by producer, by wild auxiliary part of educating by peasants and herdsmen It completes, peasants and herdsmen is allowed to support and participate in artificial breeding process, realize that enterprise, peasants and herdsmen's economic benefits and social benefits increase income, while being also able to achieve winter worm The wild and artificial combination of summer grass enhances its drug genuineness while improving output, promotes cordyceps sinensis quality.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below to the technical side in embodiment Case is clearly and completely described, and the following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..If not It refers in particular to, all parts, percentage are unit of weight.
Embodiment 1
Method is grown in the auxiliary cordyceps sinensis training for educating combination of a kind of artificial propagation and field, comprising the following steps:
Step 1, artificial breeding indoors in environment, under conditions of temperature and humidity control, successively carries out worm pupa and hatches, mates, adopts Ovum, larva culture, Spawn incubation process obtain of the right age larva, Chinese coat spore bacterium solution.Wherein, worm pupa is hatched: temperature control exists 20-25 DEG C, humid control is in 45-55%, and hatching is at moth within 20-30 days;Mating: epidemic disaster remains unchanged, and keeps quite, and avoids Mating is completed in noise effect in fly net enclosed environment;It adopts ovum: in worm moth mating place, adding 1% urea with soft wheat bran Liquid attracts worm moth natural spawning, and collects worm's ovum in time, surveys sample quantity;Egg hatch: indoor temperature is improved into 5-8 DEG C, worm's ovum is put into hatching house, to hatching rate up to 80% or more i.e. move out larva culture between;Larva culture: it is cultivated in larva Between, temperature is adjusted to 20-25 DEG C, and raising obtains of the right age larva to 3-4 age after being smashed with dedicated plant feed;Cultural hypha: will The Chinese coat spore starter kind of purified domestication is cultivated 3 periods in cultural hypha tank, and stoste is spare in 0-4 DEG C of refrigeration.People Work breeds the stage, by larva growth period environmental suitability management, solves the problems, such as to adapt to local climate difficulty, to improve children The survival rate and quality rate of worm.
Step 2, it is wild it is auxiliary educate, when of the right age larva to be launched to one section of the exercise of carry out field grow to suitable cordyceps sinensis Between, with Chinese coat spore bacterium solution carry out bacterium worm infect, and carry out include it is wild it is auxiliary educate illumination, moisture, the temperature controlled later period pipe Reason, timely collecting obtain cordyceps sinensis.Wherein, field is taken exercise: being carried out at 10 DEG C of natural temperature or more, band raising box soil is put Set no mouse, sand without miscellaneous bacteria, without other insect pests is taken exercise, cover non-woven fabrics or stalk, exercise time 5-10 days, In, soil moisture 60%;Bacterium worm is infected: Chinese coat spore bacterium solution being diluted to 0.1-0.5% concentration, imposes on larva soil by spraying Earth surface is applied, continuous 7 days at dusk daily, and discovery larva appearance is ossified, that is, shows to infect success;Final-period management: soil is kept Epidemic disaster after discovery stroma grows polypide, can suitably reduce humidity, and interval provides illumination, and 1 centimetre of stroma or more can harvest, Soil and coating are brushed away after harvest, obtain cordyceps sinensis.It is wild it is auxiliary educate the stage, by larva field infect management, it is auxiliary educate management, Infection rate is high under indoor conditions by no means for solution, the undesirable problem of cordyceps sinensis growing environment, promotes the quality of cordyceps sinensis.
The present invention, which grows cordyceps sinensis training, is divided into two stages, is controlled using two stage differentiation, had both been able to achieve winter worm The segmentation of summer grass cultivates, and artificial breeding part is completed by producer, by it is wild it is auxiliary educate part and completed by peasants and herdsmen, allow peasants and herdsmen's branch Hold and participate in artificial breeding process, realize that enterprise, peasants and herdsmen's economic benefits and social benefits increase income, at the same be also able to achieve cordyceps sinensis it is wild with it is artificial Combination enhance its drug genuineness while improving output, promote cordyceps sinensis quality.
The above is only presently preferred embodiments of the present invention, is not intended to limit the present invention in any form, though So the present invention has been disclosed as a preferred embodiment, and however, it is not intended to limit the invention, any technology people for being familiar with this patent Member without departing from the scope of the present invention, when the technology contents using above-mentioned prompt make it is a little change or be modified to The equivalent embodiment of equivalent variations, but anything that does not depart from the technical scheme of the invention content, it is right according to the technical essence of the invention Any simple modification, equivalent change and modification made by above embodiments, in the range of still falling within the present invention program.

Claims (3)

1. method is grown in the auxiliary cordyceps sinensis training for educating combination of a kind of artificial propagation and field, which comprises the following steps:
Step 1, artificial breeding, indoors in environment, under conditions of temperature and humidity control, successively carry out worm pupa hatch, mate, adopt ovum, Larva culture, Spawn incubation process obtain of the right age larva, Chinese coat spore bacterium solution;
Step 2, it wild auxiliary carry out field exercise a period of time educated, of the right age larva dispensing to suitable cordyceps sinensis is grown, uses Chinese coat spore bacterium solution is carried out bacterium worm and is infected, and carry out include it is wild it is auxiliary educate illumination, moisture, temperature controlled final-period management, fit Shi Caishou obtains cordyceps sinensis.
2. method, feature are grown in the auxiliary cordyceps sinensis training for educating combination of a kind of artificial propagation according to claim 1 and field It is, in the step 1,
The hatching of worm pupa: temperature control is at 20-25 DEG C, and humid control is in 45-55%, and hatching is at moth within 20-30 days;
Mating: epidemic disaster remains unchanged, and keeps quite, and avoids noise effect, and mating is completed in fly net enclosed environment;
It adopts ovum: in worm moth mating place, adding 1% urea solution to attract worm moth natural spawning with soft wheat bran, and collect worm in time Ovum surveys sample quantity;
Egg hatch: improving 5-8 DEG C for indoor temperature, worm's ovum be put into hatching house, moves out to hatching rate up to 80% or more To between larva culture;
Larva culture: between larva culture, temperature is adjusted to 20-25 DEG C, is raised after being smashed with dedicated plant feed to 3-4 age, Obtain of the right age larva;
Cultural hypha: the Chinese coat spore starter kind of purified domestication is cultivated into 3 periods in cultural hypha tank, stoste is in 0- 4 DEG C of refrigerations are spare.
3. method, feature are grown in the auxiliary cordyceps sinensis training for educating combination of a kind of artificial propagation according to claim 1 and field It is, in the step 2,
Field take exercise: carried out at 10 DEG C of natural temperature or more, band raising box soil, be placed into no mouse, without miscellaneous bacteria, without other insect pests Sand take exercise, cover non-woven fabrics or stalk, exercise time 5-10 days, wherein soil moisture 60%;
Bacterium worm is infected: Chinese coat spore bacterium solution being diluted to 0.1-0.5% concentration, larva soil surface is imposed on by spraying, is close to daily Evening application, continuous 7 days, discovery larva appearance was ossified, that is, showed to infect success;
Final-period management: after keeping soil epidemic disaster, discovery stroma to grow polypide, can suitably reduce humidity, and interval provides illumination, 1 centimetre of stroma or more can harvest, and soil and coating are brushed away after harvest, obtains cordyceps sinensis.
CN201910037806.0A 2019-01-16 2019-01-16 Method is grown in the auxiliary cordyceps sinensis training for educating combination of a kind of artificial propagation and field Pending CN109644776A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020087193A (en) * 2001-05-14 2002-11-22 주식회사 엔에스케이텍 Liquid cultivation method of cordyceps sinensis
CN101412968A (en) * 2007-10-20 2009-04-22 朱斗锡 Novel method for increasing yield of Tibetan plateau aweto
CN102696555A (en) * 2012-06-13 2012-10-03 重庆市中药研究院 Semi-wild manual Cordyceps-cultivating method
CN103548779A (en) * 2013-11-15 2014-02-05 重庆市中药研究院 Outdoor breeding method for ophiocordyceps sinensis Berk. G.H. Sung et al. host hepialus armorieanus oberthur larvae
CN105075667A (en) * 2015-08-25 2015-11-25 四川藏宝虫草生物科技有限公司 Method used for artificially cultivating Chinese caterpillar fungus in low altitude region
CN105941340A (en) * 2016-05-25 2016-09-21 浙江农林大学 An artificial culture method for ghost moth larvae, the hosts of ophiocordyceps sinensis

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020087193A (en) * 2001-05-14 2002-11-22 주식회사 엔에스케이텍 Liquid cultivation method of cordyceps sinensis
CN101412968A (en) * 2007-10-20 2009-04-22 朱斗锡 Novel method for increasing yield of Tibetan plateau aweto
CN102696555A (en) * 2012-06-13 2012-10-03 重庆市中药研究院 Semi-wild manual Cordyceps-cultivating method
CN103548779A (en) * 2013-11-15 2014-02-05 重庆市中药研究院 Outdoor breeding method for ophiocordyceps sinensis Berk. G.H. Sung et al. host hepialus armorieanus oberthur larvae
CN105075667A (en) * 2015-08-25 2015-11-25 四川藏宝虫草生物科技有限公司 Method used for artificially cultivating Chinese caterpillar fungus in low altitude region
CN105941340A (en) * 2016-05-25 2016-09-21 浙江农林大学 An artificial culture method for ghost moth larvae, the hosts of ophiocordyceps sinensis

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Application publication date: 20190419

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