CN109633189A - Fluorescence standard card and test method for fluorescence immunity analyzer calibration and Quality Control - Google Patents
Fluorescence standard card and test method for fluorescence immunity analyzer calibration and Quality Control Download PDFInfo
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- CN109633189A CN109633189A CN201910104547.9A CN201910104547A CN109633189A CN 109633189 A CN109633189 A CN 109633189A CN 201910104547 A CN201910104547 A CN 201910104547A CN 109633189 A CN109633189 A CN 109633189A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00594—Quality control, including calibration or testing of components of the analyser
- G01N35/00693—Calibration
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00594—Quality control, including calibration or testing of components of the analyser
- G01N35/00613—Quality control
- G01N35/00623—Quality control of instruments
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6497—Miscellaneous applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00594—Quality control, including calibration or testing of components of the analyser
- G01N35/00613—Quality control
- G01N35/00623—Quality control of instruments
- G01N2035/00633—Quality control of instruments logging process history of individual samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00594—Quality control, including calibration or testing of components of the analyser
- G01N35/00693—Calibration
- G01N2035/00702—Curve-fitting; Parameter matching; Calibration constants
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Abstract
The invention discloses a kind of fluorescence standard cards for fluorescence immunity analyzer calibration and Quality Control, including fluorescence standard card ontology and fluorescence detection unit, fluorescence detection unit to be made by fluorescent solutions, photocuring fluorescent material, fluorescent ink or fluorescent ink.The fluorescence standard card is compared with using quality-control product, reference material or standard items, with luminosity stabilization, it is not easy to decay at any time, Line-width precision height, fluorescent material uniform spatial distribution, the feature that the amount and fluorescence intensity of fluorescent material are linear and preparation consistency is good.Also disclose a kind of test method, establish the method and parameter of a kind of fluorescence standard card assessment equipment, this method assesses content intact, easily operated, it is the measurement evaluation scheme of set of system, be conducive to carry out fluorescence immunity analyzer calibration and Quality Control, improve the detection accuracy of fluorescence immunity analyzer and the scheme of tracing to the source of each method, can reliably guarantee fluorescence standard be stuck in production and use process itself attribute it is rigorously controlled.
Description
Technical field
The invention belongs to fluoroimmunoassay technical fields, relate in particular to a kind of for fluorescence analyser calibration and matter
The fluorescence standard card and test method of control.
Background technique
Fluoroimmunoassay technology is to develop earliest one kind in immuno-labelling technique, is by the special of antigen-antibody reaction
Property with the sensibility of fluorescent material detection and a kind of method for combining of intuitive.Its principle are as follows: antigen, antibody will not be influenced
Active fluorochrome mark is on antibody (or antigen), after its corresponding antigen (or antibody) combines, in fluorescence microscope
Under a kind of reaction of specificity fluorescent is presented.It is excellent that fluorescence immunoassay detection technique has that specificity is strong, high sensitivity, practicability are good etc.
Point can be used for the very low bioactive compound of detection level, as protein (enzyme, acceptor, antibody), hormone, drug and
Microorganism etc..
Currently, generallyd use in industry fluorescence immunity analyzer carry out fluoroimmunoassay detection, instrument due to itself because
The interference of element and external environment, testing result can have certain deviation sometimes, for the accuracy for promoting instrument detection data, make
Before instrument, need to carry out instrument calibration and Quality Control.Calibration and appraisal procedure to device performance parameters generally comprise following
It is several: (1) to be formulated as solution using nanocrystalline equal fluorescent materials, solution is printed or sprays or is solidificated in by the methods of gel
Production becomes standard card on substrate, measures the performance parameter with assessment equipment to equipment using the standard card；(2) it uses
LED light or lamp bar are fabricated to standard card, measure the performance parameter with assessment equipment to equipment using the standard card；(3) make
Standard card is made with fluorescent minerals matter or fluorescent minerals matter mixture, equipment is measured with assessment equipment using standard card
Performance parameter.
The above method has the following technical problems: (1) nanocrystalline material solution is in the case where preparing unfavorable condition, often at any time
Between apparent decaying occurs, cause the signal of standard card to change, equipment can not be carried out reliably as stable standard items
Assessment, meanwhile, when solution carries out filament printing, Line-width precision is difficult to ensure, if solution occurs to chromatograph or be spread on substrate,
The consistency of standard card is more difficult to ensure, and is solidified by gel method, and good dispersibility is often not achieved, production
Standard card individual difference is big, can not be as the judgement object of standard.(2) LED light itself has the difference of luminous intensity and color, uses
LED light or the standard card consistency of lamp bar production are poor, in addition, LED light or lamp bar itself are light emitting sources rather than stimulated luminescence, it is difficult
It is assessed with the laser light source performance to optical module in equipment.Currently existing scheme, which generally passes through, designs single T line, passes through
The fluorescence signal intensity of T line and the relationship of solution concentration carry out linear fit, but for the design principle of fluorescence analyser,
Since the signal of final output is usually the signal by amplifying, and the multiple of signal amplification is often dynamic, such as to carry out
Linear fit then needs for multiple to be normalized, inconvenient, meanwhile, it is in the production process, low dense for single T line
The T line that degree and the solution of high concentration are made, attenuation degree often at any time is inconsistent, it is difficult to ensure that after being used for a long time,
Maintain stable linear relationship.And existing method is only illustrated the production method and structure of fluorescence standard card at present, does not have
Have the characteristic of fluorescence standard card bonding apparatus, is formed rationally and the measurement evaluation scheme of system and scheme of tracing to the source.
Summary of the invention
For this purpose, the present invention exactly to solve above-mentioned technical problem, thus propose a kind of luminescent substance it is stable, it is structurally reasonable,
Preservation and solid luminous substance can be stablized, can rational judgment equipment repeatability, stability, accuracy and linear dependence and can be into
The fluorescence standard card and test method for fluorescence analyser calibration and Quality Control that row is traced to the source.
In order to solve the above technical problems, the technical solution of the present invention is as follows:
The present invention provides a kind of fluorescence standard card for fluorescence immunity analyzer calibration and Quality Control, the fluorescence standard card
Including fluorescence standard card ontology and fluorescence detection unit, the fluorescence detection unit is by fluorescent solutions, photocuring fluorescent material, glimmering
Light ink or fluorescent ink are made.
Preferably, being installed with groove or accommodating cavity, the fluorescence at the observation window of the fluorescence standard card ontology
Solution, photocuring fluorescent material are placed in the groove or accommodating cavity；Or the fluorescent solutions, photocuring fluorescent material hold
It is placed in the runner of capillary or micro-fluidic chip, the capillary or the micro-fluidic chip are set to the observation window
Place.
Preferably, being provided with slide glass in the fluorescence standard card ontology, the slide surface production has fluorescence detection portion,
The fluorescence detection portion production has fluorescent ink goods fluorescent ink water layer, and the fluorescence detection portion is set to fluorescence standard card ontology
At observation window.
Preferably, the slide surface is additionally provided with anti-dazzling screen, the anti-dazzling screen corresponds to the fluorescence detection portion
Position is hollow out position.
There are also a kind of test methods using the fluorescence standard card by the present invention, according to fluorescence in fluorescence detection unit
Fluorescence standard card is set as 1-9 grades of gradients by the area of the concentration of matter or fluorescence detection unit, and concentration can be from 0~100%, example
Such as 0%, 1%, 5%, 10%, 20%, 30%, 50%, 60%, 90%, corresponding signal value can from 0-100000000,
Such as card 1~9 signal values of card can be with are as follows: 10,100,500,1000,5000,10000,50000,100000,500000, it uses
Fluorescence standard card with various concentration or area gradient carries out calibration test, the calibration test packet to fluorescence immunity analyzer
Include reperformance test, stability test, accuracy test and linearly related test.
Preferably, the reperformance test includes: that will distinguish with the fluorescence standard card of 1-9 grades of concentration or area gradient
The detection mouth of insertion fluorescence immunity analyzer continuously measures ten times, calculates each fluorescence standard card measurement result according to following formula
The coefficient of variation, according to the coefficient of variation determine fluorescence immunity analyzer repeatability:Wherein, s
For the standard deviation of sample test value；For the average value of sample test value.
Preferably, the stability test include: take the 1st, 5, the fluorescence standard card of 9 concentration or area gradient inserts respectively
Enter continuously to measure 3 times in the card slot of fluorescence immunity analyzer, 4, measure again respectively 3 times after 8h, being averaged for every 3 measurements is taken respectively
Value is used as measured value, and the average value of initial 3 measurements is as a reference value, according to following formula computational stability coefficient:Wherein,The average values of 3 measurements when for 4h, 8h；For the average value of first 3 times measurements of beginning；
Accuracy test include: take the 1st, 5, the fluorescence standard card of 9 concentration or area gradient is inserted into fluorescence respectively and exempts from
Each continuous measurement 3 times in the card slot of epidemic disease analyzer, take the average value of every 3 measurements as each measured value, respectively with fluorescence
The nominal value of standard card is true value, and the coefficient of accuracy of measurement is calculated according to following formula:Wherein, xiFor
Each measured value, n are pendulous frequency, and X is true value.
Preferably, the linear dependence test includes: the fluorescence standard card for taking T/C value as different gradients, it is described glimmering
The order of magnitude of the gradient of the nominal value of light standard card is not less than 2, and gradient magnitude is not less than 5, and the fluorescence standard card is inserted respectively
Enter fluorescence immunity analyzer continuously to measure 3 times, calculate per the average value measured three times, and calculates linear relationship according to following formula
Coefficient:Wherein, XiFor the nominal value of fluorescence standard card；YiFor
Standard card measures the average value of 3 measured values.
Preferably, further include the steps that fluorescence standard card is traced to the source before calibration test, fluorescence in the fluorescence standard card
Substance is fluorescent solutions or photocuring fluorescent material, according to the requirement that linear test concentrations match, by the fluorescent solutions or light
Solidification fluorescent material is diluted to 1-9 grades of concentration gradients, and the fluorescent solutions of various concentration gradient or photocuring fluorescent material are distinguished
It injects in the card slot or accommodating cavity of fluorescence standard card, obtains the fluorescence standard card of various concentration gradient；Or using same concentration
Fluorescent material is injected separately into card slot or accommodating cavity that area is 1-9 grades, the different test strip of area is obtained, by above-mentioned
Step of tracing to the source is traced to the source.
Preferably, further include the steps that fluorescence standard card is traced to the source before calibration test, fluorescence in the fluorescence standard card
Substance is fluorescent ink or fluorescent ink, and fluorescent ink or fluorescent ink are prepared in slide surface, according to fluorescent ink or glimmering
The concentration of fluorescent material is prepared as 1-9 grades of fluorescence color lumps in light ink, irradiates the fluorescence color lump with standard sources and measures institute
The brightness for stating color lump takes 3 times and measures brightness measurements of the obtained average value as color lump, when the brightness measurement of fluorescence color lump
Relative deviation is then installed on fluorescence standard card ontology less than 1% between the nominal value that value and color lump define；Or use same fluorescence
Matter concentration prepares the fluorescence color lump of 1-9 grades of areas, is traced to the source by above-mentioned step of tracing to the source.
The above technical solution of the present invention has the following advantages over the prior art:
(1) the fluorescence standard card of the present invention for fluorescence immunity analyzer calibration and Quality Control, the fluorescence standard
Card includes fluorescence standard card ontology and fluorescence detection unit, and the fluorescence detection unit is consolidated by fluorescent solutions, fluorescent solutions and light
Change gluing agent composition or fluorescent ink is made.Fluorescent material in fluorescence standard card is compared with conventional fluorescent material, photism
Matter is stablized, and is not easy to decay at any time, and Line-width precision is high, and the consistency of standard card is good.It can be theoretical and in practice, by standard card
Fluorescent material and the fluorescence intensity that detects of equipment form linear relationship, can analysis and assessment instrument linear dependence.
(2) test method of the present invention, according to the concentration of fluorescent material in fluorescence detection unit by fluorescence standard card
It is set as 1-9 grades of concentration or area gradient, using the fluorescence standard card with various concentration or area gradient to fluorescence immunoassay point
Analyzer carries out calibration test, and the calibration test includes reperformance test, stability test, accuracy test and linearly related survey
Examination.The method and assessment parameter for establishing a kind of fluorescence standard card assessment equipment, additionally provide the preparation method of fluorescence standard card,
This method assesses content intact, easily operated, is the measurement evaluation scheme of set of system, be conducive to fluorescence immunity analyzer into
Row calibration and Quality Control, improve the detection accuracy of fluorescence immunity analyzer, while can be realized tracing to the source for standard card, Neng Gouke
It can be rigorous controlled by guaranteeing that fluorescence standard is stuck in production and use process attribute itself.
Detailed description of the invention
In order to make the content of the present invention more clearly understood, it below according to specific embodiments of the present invention and combines
Attached drawing, the present invention is described in further detail, wherein
Fig. 1 is the structural schematic diagram of fluorescence standard card described in the embodiment of the present invention 1；
Fig. 2 is fluorescence immunity analyzer described in the embodiment of the present invention and fluorescence standard card schematic diagram；
Fig. 3 is the structural schematic diagram of fluorescence standard card described in the embodiment of the present invention 2；
Fig. 4 is the schematic diagram that area refers to method in test method described in the embodiment of the present invention；
Fig. 5 is that fluorescent ink is traced to the source the schematic diagram of process in test method described in the embodiment of the present invention；
Fig. 6 is Quality Control curve in test method described in the embodiment of the present invention 3.
Appended drawing reference indicates in figure are as follows: 1- fluorescence standard card；11- fluorescence standard card ontology；12- fluorescence detection unit；
121- slide glass；13- observation window；14- anti-dazzling screen；2- fluorescence immunity analyzer；21- detection mouth.
Specific embodiment
Embodiment 1
The present embodiment provides a kind of fluorescence standard cards for fluorescence immunity analyzer calibration and Quality Control, referring to Fig. 1, institute
Stating fluorescence standard card 1 includes fluorescence standard card ontology 11 and fluorescence detection unit 12, and the fluorescence detection unit 12 is molten by fluorescence
Liquid, photocuring fluorescent material, fluorescent ink or fluorescent ink are made.
Specifically, the fluorescence standard card ontology 11 is organic material, metal material, inorganic non-metallic material or timber system
At shell be provided with a detection card in the observation window 13 with observation window 13, be arranged on the detection card useful
In the groove for accommodating the fluorescence detection unit 12, the groove is in long strip, is provided with fluorescent solutions, is equipped with fluorescence
The groove of solution forms detection line, and the fluorescent solutions are mixed with solvent by mechanical stirring or sonic oscillation by fluorescence radiation substance
It closes uniformly and obtains.In the present embodiment, the fluorescence radiation substance is white azoic coupling component N- (the chloro- 2- methoxyphenyl of 5-) -3- hydroxyl -
2- naphthalenecarboxamide and Ba3MgSi2O8: Eu2+,M n2+, the solvent is NaOH solution, white azoic coupling component N- (the chloro- 2- methoxy of 5-
Base phenyl) -3- hydroxyl -2- naphthalenecarboxamide be 1g, the Ba3MgSi2O8: Eu2+,M n2+For 0.5g, the NaOH solution concentration
For 1mol/L, volume 5ml.The advantages of fluorescent solutions, is that wherein fluorescent material is evenly distributed, and solution state can solve linearly
It needs optical signal carrying out corresponding problem with material concentration in measurement.
Difference is produced according to different groove widths by preparing the fluorescent solutions of various concentration, or with the solution of same concentration
The standard card of line width has the fluorescence standard card of different fluorescence signal gradients to fluorescence immunity analyzer 2 to obtain some column
The performance of (as shown in Figure 2) equipment is tested.
The fluorescence immunity analyzer 2 is using fluorescence immune chromatography quantitative detection, fluorescence immunoassay determining adsorption, time point
Distinguish one of fluorescence detection, in situ hybridization, the quantitative detection equipment of biochip grade microflow control technique.It is with detection mouth
21, the fluorescence standard card 1 is inserted into the detection mouth 21 and carries out calibration measurement to equipment.
Alternatively, the fluorescence detection unit 12 is by obtained, the photocuring fluorescence in photocuring fluorescent material injection groove
Material is above-mentioned white color by the way that fluorescent material dissolution in a solvent, to be formed to fluorescent material solution, the fluorescent material solution
Phenol N- (the chloro- 2- methoxyphenyl of 5-) -3- hydroxyl -2- naphthalenecarboxamide and Ba3MgSi2O8: Eu2+,Mn2+Sodium hydroxide solution,
A certain amount of photocuring adhesive is mixed again, and mechanical stirring or sonic oscillation, which are sufficiently mixed, to be uniformly made.The photocuring adhesive
Including photo-curing monomer, prepolymer and dispersing agent, the ratio of three is in the ratio of curing adhesive for wherein photo-curing monomer
The component ratio of 1-60%, pre-polymer ratio 1-60%, dispersing agent 1-10%, fluorescent solutions and light curing agent is 1:
0.001 arrives 1:1000.Wherein, the photo-curing monomer be bifunctional monomer TPGDA, HDDA, it is DEGDA, NPGDA, multifunctional
At least one of single group body TMPTA, PETA.The prepolymer is epoxy acrylate, urethane acrylate, polyethers propylene
At least one of acid esters, polyester acrylate, acrylic resin.The photoinitiator is 1173,184,907, benzophenone
At least one of.The photocuring adhesive obtained under mentioned component and proportion can solidify under the irradiation of ultraviolet light.
After in the fluorescent solutions and photocuring gluing agent composition injection groove, make its solidification with ultraviolet light, thus
So that fluorescent material is dispersed in cured gel, long-term preservation can be stablized.
As disposable embodiment, the groove may be an accommodating cavity, and the accommodating cavity surface has transparent
Window, in order to observe fluorescence detection unit.The fluorescent solutions or fluorescent solutions can also be first with photocuring gluing agent composition
It is flowed into the runner of capillary or micro-fluidic chip, then the capillary or micro-fluidic chip is placed in groove, or is set
At observation window 13.
Embodiment 2
The present embodiment provides a kind of fluorescence standard cards for fluorescence immunity analyzer calibration and Quality Control, referring to Fig. 3, its
Including fluorescence standard card ontology 1 and fluorescence detection unit 12, the fluorescence detection unit 12 by fluorescent ink or fluorescent ink,
Fluorescent pigment is made, and antiager and stabilizer are contained in fluorescent ink, fluorescent ink, and fluorescent material therein has good
Dispersibility and granulation uniformity have higher stability compared with other conventional fluorescent materials.It is set to the fluorescence standard
In card ontology 1.In the present embodiment, the fluorescence detection unit 12 includes a slide glass 121, and surface printing or printing and making have glimmering
Optical detection part, the fluorescence detection portion are the fluorescent ink layer or fluorescent ink water layer of printing or printing, and the fluorescence detection portion can
It is observed from observation window 13.Further, 121 surface of slide glass is additionally provided with an anti-dazzling screen 14, the anti-dazzling screen pair
The position in fluorescence detection portion described in Ying Yu is hollow out position, blocks fluorescent ink test section by anti-dazzling screen, the effect of anti-dazzling screen
It is to make the zone boundary of hollow out position light leakage neat, so that the region consistency of light leakage is high.
The fluorescence detection unit 12 can be firsthand fluorescence colour atla, can also be and print fluorescent ink by lithographic plate
The techniques such as brush, intaglio printing, letterpress, porous printing, flexible version printing, silk-screen printing and inkjet printing are in slide glass 121
Surface prepares fluorescence striped (fluorescent ink detection line) or fluorescent ink detects color lump, and the slide glass is art paper, offset paper, gold
Belong to the textiles, glass such as (such as aluminium foil, stainless steel plate), plastics (such as PVC, PET), adhesive sticker paster, ventilated membrane, non-woven fabrics
The materials such as glass, ceramics or wood, shading sheet material can be plastics, metal etc., be cut by machining, laser cutting or line
Mode makes, and can keep very high precision size degree, guarantees the consistency and accuracy of standard card test signal window.
Embodiment 3
The present embodiment, which also provides, a kind of carries out school to fluorescence immunity analyzer using fluorescence standard card described in embodiment 1,2
Quasi- and Quality Control test method, utilizes relationship, the relationship of fluorescence intensity and light-emitting area of fluorescence intensity and fluorescent material concentration
Principle is respectively adopted concentration and assesses with reference to method equipment performance with reference to method and area.
1, concentration refers to method
Within the scope of a certain concentration, the solution concentration and light intensity of fluorescent material are (Lambert-Beer's law) in a linear relationship, when
When using fluorescent solutions and photocuring fluorescent material production fluorescence standard card, the solid space of fluorescent material curing agent after hardening
Interior degree of scatter is constant, i.e. the concentration in space is constant, since photocuring adhesive is transparency material, does not influence the transmission of light.Cause
This, this fluorescence standard card can form the linear relationship of concentration and light intensity.When using fluorescent ink (or fluorescent ink, fluorescence face
Material) production fluorescence standard card when, fluorescence detection line or fluorescence color lump are obtained, since fluorescent ink have passed through point in the production process
It dissipates or mixes, after printing or printing to 121 surface of slide glass, fluorescent material is also uniformly distributed.
Pass through the fluorescent solutions and optic-solidified adhesive of the light intensity for testing equipment and a series of concentration for making standard card
Glutinous agent composition or fluorescent ink correspondence analysis, can be fitted to obtain the linear relationship of measurement signal value and concentration, to rationally comment
Estimate the measurement linearity of equipment.
2, area refers to method
Solid matter is prepared using fluorescent solutions and photocuring gluing agent composition or fluorescent ink, is dispersed in fluorescent material
In good situation, surface unit area fluorescent material be may be considered uniformly, can be by fluorescent solutions and photocuring gluing
Agent or fluorescent ink support a series of bands according to different length-width ratios, and in the present embodiment, fluorescent bands width is 5mm, high
Degree is respectively 0.05mm, 0.1mm, 0.3mm, 0.5mm, 1mm, 2mm, 2.5mm, 3mm and 5mm (as shown in Figure 4).It is minimum and maximum
The strip width ratio of height is 100, when strip face fluorescent material is evenly distributed and in the identical situation of concentration, unit area
The fluorescence signal value that band issues is theoretically identical, when standard card passes through optical module bottom in an identical manner and carries out
When scanning, the fluorescence signal value that takes for the item that optical module receives by be unit area fluorescence signal integral.Such as:
When the height of standard card fluorescent bands is respectively 0.1mm (standard card 1) and 2mm (standard card 2), two standard cards are respectively with phase
When same speed passes through from fluorescence immunity analyzer optical module bottom, in the exciting light situation identical with reception device state
Under, when optical module scanning card 2, it is believed that optical module continuously has read the fluorescence of 20 standard cards 1 with identical condition
Signal, i.e. fluorescence measurement value are the integrated value of 20 standard cards 1, therefore, the height of fluorescent bands or the area of band and equipment
The fluorescence signal value detected is directly proportional.Width correspondence analysis by the light intensity and band that measure equipment, can be fitted to obtain
The linear relationship of measurement signal value and concentration, to rationally assess the measurement linearity of measuring device.
Before production fluorescence standard card and test, the operation of tracing to the source of fluorescence detection unit is carried out first, when fluorescent material is
When fluorescent solutions or photo-curing material, tracing to the source for fluorescent solutions stoste is carried out first: it is (such as white to purchase or prepare fluorescent material raw material
Color azoic coupling component N- (the chloro- 2- methoxyphenyl of 5-) -3- hydroxyl -2- naphthalenecarboxamide and Ba3MgSi2O8: Eu2+,Mn2+), by fluorescent material
Starting material with water or other solvents are formulated as the fluorescent solutions stoste that concentration is 5mg/mL, using sepectrophotofluorometer to preparation
The luminous intensity of fluorescent solutions stoste measures, when the luminous intensity of fluorescent solutions stoste and the deviation of standard value of preparation exist
Within 5%, fluorescent solutions or photocuring fluorescent material are made with it.
The source tracing method of fluorescent solutions or photocuring fluorescent material are as follows:, will be upper according to the requirement that existing test concentrations match
It states fluorescent solutions stoste water or other solvents is diluted to the fluorescent solutions or photocuring fluorescent material of various concentration, it will be different dense
The fluorescent solutions of degree are filled into fluorescence standard card, the fluorescence standard card with various concentration ratio are obtained, using fluorescence obtained
Standard card measures the linear dependence of fluorescence immunity analyzer.Specifically, production has the fluorescence of 1-9 grades of concentration gradients
Fluorescent material in the fluorescence detection unit of fluorescence standard card (fluorescent solutions or photocuring fluorescent material) is made as by standard card
9 grades of concentration from low to high, in the present embodiment, concentration is respectively 0%, 1%, 5%, 10%, 20%, 30%, 50%, 60%,
90%, or the fluorescent bands (as shown in Figure 4) that 1-9 level width is identical, height is different are prepared with the fluorescent material of same concentration,
And then obtain the fluorescence standard card of 9 kinds of fluorescence signal gradients, corresponding signal value can for 10,100,500,1000,5000,
10000,50000,100000,500000, method is referred to reference to method or area according to concentration, using the glimmering of above-mentioned various concentration gradient
Light standard card carries out calibration test to fluorescence immunity analyzer.
In the preparation process of fluorescence standard card, the structure for accommodating fluorescent material can be to be set to fluorescence standard card
The groove on 1 surface of ontology, can make the fluorescent material that groove area is identical, compound concentration is different, obtain one with concentration gradient
Group fluorescence standard card, can also make fluorescent material concentration identical, one group of different fluorescence standard card of production groove area.The fluorescence
Substance can be fluorescent solutions, obtain liquid phase standard card, can also (photocuring is glimmering for the mixture of fluorescent solutions and photocuring adhesive
Luminescent material), when its filling is after in groove, makes its solidification with ultraviolet light, form card slot and solidify fluorescence standard card.
Structure for accommodating fluorescent material can also be capillary, and fluorescent material is fluorescent solutions or photo-curing material, system
When making, capillary (such as capillary of capillary heparin tube or quartz, transparent plastics material) is inserted into the fluorescent solutions or light is solid
Change in material, liquid forms continuous liquid section since capillary effect is drawn into capillary, when fluorescent material is photocuring material
When material, with ultraviolet light capillary, solidify mixture therein, be stored in capillary, luminescent material is after hardening
Dispersity is kept in mixture, and capillary is intercepted into one section (such as 5mm), by it at the observation window 13 of standard card, is obtained
Diameter to capillary tube solidification fluorescence standard card, capillary is similar with detection width of fringe.According to fluorescent material concentration difference system
Standby is the fluorescence standard card (1-9 grades) of various concentration, or is prepared as different line widths or sectional area according to capillary diameter difference
Fluorescence standard card.
Structure for accommodating fluorescent material can also be the runner of micro-fluidic chip, and fluorescent material is filled by injection needle
Into the runner of micro-fluidic chip, continuous liquid section is formed.Fluorescent material is the mixture of fluorescent solutions and photocuring adhesive, purple
After outer solidification, fluorescent material solidifies in runner, and the fluorescence standard card of various concentration is prepared as according to fluorescent material concentration difference
(1-9 grades), or it is prepared as according to width of flow path difference the fluorescence standard card of different line widths or sectional area.
Alternatively, the fluorescent material be fluorescent ink, slide glass 121 (art paper/offset paper/plastic tab/sheet glass/
Sheet metal) on printing prepare fluorescent bands, which is cut and pastes standard card surface or is placed in slide glass
Inside fluorescence standard card, observation window 13 is exposed in fluorescent bands part, when using fluorescent ink, without making groove or accommodating
Chamber, capillary etc..Can also directly fluorescent ink print on 1 surface of fluorescence standard card.1-9 grades of concentration ladders are prepared according to printing ink concentration
The fluorescence standard card of degree, or the fluorescent bands of different in width are prepared with same concentration fluorescent ink printing, obtain fluorescence standard card.
The fluorescent material is the source tracing method of fluorescent ink are as follows: prepares fluorescence color lump, foundation using fluorescent ink printing
The concentration of fluorescent material prints to different fluorescence color lumps in fluorescent ink or ink, and in the present embodiment, fluorescence color lump is 9, according to
The secondary concentration (such as 0%, 1%, 5%, 10%, 20%, 30%, 50%, 60%, 90%) for corresponding to 9 fluorescent materials, color lump system
After standby, with standard sources (UV- ultraviolet source, wavelength: 365nm, power: 200W) with 45 degree of angular illumination fluorescent bands, use
For opacimeter in the fluorescent brightness measured value (as shown in Figure 5) of the position of vertical fluorescence color lump measurement color lump transmitting, when measurement, can
Optical filter is used to filter interference light, optical filter wavelength is 615nm, half-peak breadth 40nm, takes 3 calculations for measuring obtained brightness value
Final testing result of the number average value as the fluorescence color lump, measures 9 fluorescence color lumps, and mark with defined in band respectively
Title value compares, and relative deviation is less than 5%, then the fluorescent ink with above-mentioned fluorescence intensity can be prepared as in fluorescence standard card and appropriate
It is kind save, be moisture-proof, anti-pollution, anti-long-time strong illumination.Fluorescent ink or fluorescent ink are printed as what color lump was tested
Reason is mainly that the hot spot of standard sources is that have certain area, needs certain color lump area that could accommodate hot spot to carry out
Test.By same printing ink to manufacture color lump and production standard card, as long as color lump controls its signal by this method, so that it may pass through
This method is controlled and is traced to the source to the technique and performance parameter of the fluorescent bands of standard card.
When the fluorescent material be fluorescent ink, the fluorescence of various concentration or different area gradient is prepared by inkjet printing
Band, fluorescence standard card preparation process and process of tracing to the source with fluorescent ink are essentially identical with fluorescent ink.
Furthermore it is also possible to which anti-dazzling screen is arranged in slide surface, the fluorescence standard card of sandwich structure is obtained.
Calibration test to fluorescence immunity analyzer includes reperformance test, stability test, accuracy test and linear
Four indexs of dependence test:
(1) reperformance test refers to the degree for being repeated several times and being consistent each other between each measured value when measuring same amount, indicates
The size of random error in continuous mode.Taking concentration gradient (or area gradient) is that 1-9 grades of fluorescence standard card is inserted into sets respectively
Continuously measurement 10 times of standby detection mouth 21, read measured value, calculate each fluorescence standard card measurement result according to following formula
The coefficient of variation (CV, %) determines the repeatability of fluorescence immunity analyzer according to the coefficient of variation:
Wherein, s is the standard deviation of sample test value；For the average value of sample test value, it as a result should meet and be related to the requirement of parameter value.
(2) stability test after referring to separated in time, tests the same object, the difference between front and back measurement numerical value
It is different, indicate the measurement degree of stability of system.Take the 1st, 5, the fluorescence standard card of 9 concentration or area gradient be inserted into fluorescence immunoassay respectively
Continuously measure 3 times in the card slot of analyzer, 4, measure again respectively 3 times after 8h, take the average values of every 3 measurements as measurement respectively
Value, the average value of initial 3 measurements is as a reference value, according to following formula computational stability coefficient (a, %):Wherein,The average values of 3 measurements when for 4h, 8h；For being averaged for first 3 times measurements of beginning
Value.
(3) the accuracy test refers to the journey that the average value repeatedly measured under certain experiment condition is consistent with true value
Degree indicates that it is used to indicate the size of systematic error with relative error.Include: take the 1st, 5,9 concentration or area gradient it is glimmering
Light standard card is inserted into each continuous measurement 3 times in the card slot of fluorescence immunity analyzer respectively, and the average value of every 3 measurements is taken to make respectively
It compares to obtain its measurement with the test result of machine to be measured using the nominal value of standard card as true value for each measured value
Accuracy calculates the coefficient of accuracy of measurement according to following formula:Wherein, xiFor each measured value, n
For pendulous frequency, X is true value.
(4) linear dependence, in the measured value range not less than 2 orders of magnitude, linearly dependent coefficient (r) >=0.99.
Taking T/C value is the fluorescence standard card of different gradients, and the order of magnitude of the gradient of the nominal value of the fluorescence standard card is not less than 2, ladder
Degree amount is not less than 5, and the fluorescence standard card is inserted into fluorescence immunity analyzer respectively and is continuously measured 3 times, and calculating is every to survey three times
The average value (Yi) of amount, and linear relationship coefficient is calculated according to following formula:
Wherein, XiFor the nominal value of fluorescence standard card；YiFor the average value of 3 measured values of standard card measurement.
Fluorescence nominal value is by standard sources and standard meter (such as opacimeter or light power meter) by anti-
Repetition measurement measures assembly average and obtains, or the numerical value obtained by theoretical calculation.It is surveyed by using standard sources and standard
The signal value for measuring large stretch of color lump of instrument measurement preparation, as the nominal value for the fluorescence standard card that same batch is produced, for checking
Whether the quality and parameter with the standard card that the batch is produced of tracing to the source are in use scope.
In above-mentioned parameter, assigned when the true value of accuracy and linear dependence is by preparing the fluorescence standard card according to technique
Signal value, measured measured value and true value are carried out to calculate relative deviation and linear correlation, it can be estimated that equipment
The performance parameter of itself.Fluorescence immunity analyzer is measured by using the fluorescence standard locking phase, and is recorded every time
Measurement data, the Quality Control data of available equipment measurement performance itself, and the equipment Quality Control software function carried by analyzer
Energy recording and displaying comes out (as shown in Figure 6), and the present embodiment is distinguished by detecting the Quality Control of equipment performance Quality Control and reagent card
It carries out, in conjunction with the fluorescence standard card, easily and reliably the operating status of equipment itself is detected, is determining equipment sheet
The Quality Control of reagent card is carried out on the basis of body is functional again, two data acquisitions are independent mutually, but detect the matter of whole system
Amount monitoring is assisted mutually again, is complemented one another, for monitoring device from design, produce, use and repair etc. whole process have it is heavy
It acts on.
Obviously, the above embodiments are merely examples for clarifying the description, and does not limit the embodiments.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or
It changes.There is no necessity and possibility to exhaust all the enbodiments.And it is extended from this it is obvious variation or
It changes still within the protection scope of the invention.
Claims (10)
1. a kind of fluorescence standard card for fluorescence immunity analyzer calibration and Quality Control, which is characterized in that the fluorescence standard card
Including fluorescence standard card ontology and fluorescence detection unit, the fluorescence detection unit is by fluorescent solutions, photocuring fluorescent material, glimmering
Light ink or fluorescent ink are made.
2. the fluorescence standard card according to claim 1 for fluorescence immunity analyzer calibration and Quality Control, which is characterized in that
Groove or accommodating cavity, the fluorescent solutions, photocuring phosphor are installed at the observation window of the fluorescence standard card ontology
Material is placed in the groove or accommodating cavity；Or the fluorescent solutions, photocuring fluorescent material are placed in capillary or miniflow
In the runner for controlling chip, the capillary or the micro-fluidic chip are set at the observation window.
3. the fluorescence standard card according to claim 1 for fluorescence immunity analyzer calibration and Quality Control, which is characterized in that
Slide glass is provided in the fluorescence standard card ontology, the slide surface production has fluorescence detection portion, fluorescence detection portion system
Work has fluorescent ink or fluorescent ink water layer, and the fluorescence detection portion is set at the observation window of fluorescence standard card ontology.
4. the fluorescence standard card according to claim 3 for fluorescence immunity analyzer calibration and Quality Control, which is characterized in that
The slide surface is additionally provided with anti-dazzling screen, and the position that the anti-dazzling screen corresponds to the fluorescence detection portion is hollow out position.
5. a kind of test method using fluorescence standard card according to any one of claims 1-4, which is characterized in that according to glimmering
Fluorescence standard card is set as 1-9 grades of gradients by the area of the concentration of fluorescent material or fluorescence detection unit in optical detecting unit, is adopted
Calibration test, the calibration test are carried out to fluorescence immunity analyzer with the fluorescence standard card with various concentration or area gradient
Including reperformance test, stability test, accuracy test and linearly related test.
6. test method according to claim 5, which is characterized in that the reperformance test includes: will be dense with 1-9 grades
The detection mouth that degree or the fluorescence standard card of area gradient are inserted into fluorescence immunity analyzer respectively continuously measures ten times, according to following public affairs
Formula calculates the coefficient of variation of each fluorescence standard card measurement result, and the weight of fluorescence immunity analyzer is determined according to the coefficient of variation
Renaturation:Wherein, s is the standard deviation of sample test value；For the average value of sample test value.
7. test method according to claim 6, which is characterized in that the stability test includes: to take the 1st, 5,9 concentration
Or the fluorescence standard card of area gradient is inserted into the card slot of fluorescence immunity analyzer respectively and continuously measures 3 times, 4, after 8h respectively again
Measurement 3 times takes the average values of every 3 measurements as measured value respectively, the initial 3 times average values measured as a reference value, according to
Following formula computational stability coefficient:Wherein,The average values of 3 measurements when for 4h, 8h；For the average value of first 3 times measurements of beginning；Accuracy test include: take the 1st, 5, the fluorescence standard of 9 concentration or area gradient
Card is inserted into each continuous measurement 3 times in the card slot of fluorescence immunity analyzer respectively, takes the average value of every 3 measurements as each respectively
Measured value, using the nominal value of fluorescence standard card as true value, according to following formula calculate measurement coefficient of accuracy:Wherein, xiFor each measured value, n is pendulous frequency, and X is true value.
8. test method according to claim 7, which is characterized in that linear dependence test includes: to take the T/C value to be
The order of magnitude of the fluorescence standard card of different gradients, the gradient of the nominal value of the fluorescence standard card is not less than 2, and gradient magnitude is not small
In 5, the fluorescence standard card is inserted into fluorescence immunity analyzer respectively and is continuously measured 3 times, calculated per the average value measured three times,
And linear relationship coefficient is calculated according to following formula:Wherein,
XiFor the nominal value of fluorescence standard card；YiFor the average value of 3 measured values of standard card measurement.
9. test method according to claim 8, which is characterized in that further include that fluorescence standard card is traced to the source before calibration test
The step of, fluorescent material is fluorescent solutions or photocuring fluorescent material in the fluorescence standard card, is matched according to linear test concentrations
The fluorescent solutions or photocuring fluorescent material are diluted to 1-9 grades of concentration gradients, by the glimmering of various concentration gradient by the requirement of ratio
Light solution or photocuring fluorescent material are injected separately into the card slot or accommodating cavity of fluorescence standard card, obtain the glimmering of various concentration gradient
Light standard card；Or the fluorescent material using same concentration, it is injected separately into card slot or accommodating cavity that area is 1-9 grades, obtains face
The different test strip of product, is traced to the source by above-mentioned step of tracing to the source.
10. test method according to claim 8, which is characterized in that further include that fluorescence standard card traces back before calibration test
The step of source, fluorescent material is fluorescent ink or fluorescent ink in the fluorescence standard card, by fluorescent ink or fluorescent ink system
For in slide surface, 1-9 grades of fluorescence color lumps are prepared as according to the concentration of fluorescent material in fluorescent ink or fluorescent ink, use standard
Light source irradiates the fluorescence color lump and measures the brightness of the color lump, takes 3 times and measures obtained average value as the bright of color lump
Measured value is spent, when relative deviation is then installed on glimmering less than 5% between the nominal value that the brightness measurements and color lump of fluorescence color lump define
Light standard card ontology；Or same fluorescent material concentration is used, the fluorescence color lump of 1-9 grades of areas is prepared, above-mentioned step of tracing to the source is passed through
It traces to the source.
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| PCT/CN2019/076036 WO2020155241A1 (en) | 2019-02-01 | 2019-02-25 | Fluorescence standard card for calibration and quality control of fluorescence immunoassay analyzer and test method |
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