CN208043837U - A kind of corrector strip for the calibration of dry type immunity analysis instrument - Google Patents
A kind of corrector strip for the calibration of dry type immunity analysis instrument Download PDFInfo
- Publication number
- CN208043837U CN208043837U CN201721463906.2U CN201721463906U CN208043837U CN 208043837 U CN208043837 U CN 208043837U CN 201721463906 U CN201721463906 U CN 201721463906U CN 208043837 U CN208043837 U CN 208043837U
- Authority
- CN
- China
- Prior art keywords
- raceway groove
- corrector strip
- calibration
- dry type
- analysis instrument
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model discloses a kind of corrector strips for the calibration of dry type immunity analysis instrument, the corrector strip is made of upper cover plate and the conjunction of lower cover lid, sample introduction window and venthole are provided on the upper cover plate, the lower cover is provided with raceway groove, the raceway groove at least one end has accommodating chamber, wherein, an accommodating chamber of the raceway groove of lower cover is corresponded to when combining installation above and below position with the sample introduction window of upper cover plate.The corrector strip of the utility model has the raceway groove of different shape and working depth, by the way that different liquid dyes and dye strength are added, it can be achieved that the debugging of the functions such as the assessment of dry type immunity analysis instrument automatic sample, automatic dilution assessment, quantitative detection is calibrated.
Description
Technical field
The utility model belongs to instrument calibration technical field, is related to a kind of corrector strip of special designing, through specific dye is added
Material, can be used for the evaluation of alignment of dry type immunity analysis instrument.
Background technology
Dry type immunity analysis instrument is answered extensively as a kind of POCT (point-of-care testing, real-time test) equipment
For biomedical inspection field.The mark substance that can be used for quantitative analysis determinand is varied, from colloidal gold, color latex
Microballoon, to fluorescent microsphere, quantum dot etc..However when reality calibrates equipment we have found that following problem：1, at present
Corrector strip for calibration is mostly dry plate, it is impossible to be used in liquid channel system assessment, dilution assessment and whole system evaluation；2, for calibrating
Corrector strip itself there are unstable, such as fluorescence dry plate, quantum dot dry plate；3, for the corrector strip of calibration, there is also more serious
Non-uniform problem, such as the gray scale piece of inkjet printing.Piece calibration and standardization point of the above problem in dry type immunity analysis instrument
Larger interference is generated when analysis.
And the conventional cuvette hardly possible for liquid channel system meets the ultra-thin of dry type immunity analysis instrument requirement on thickness
The requirement of standard film.The apparent thickness of standard film is 3.2~3.7mm, indeed according to different demands can with flexible design, but
Cuvette is fixed structure, apparent thickness should in 1cm or so, if using the cuvette of minimum volume, apparent thickness
It is constant.Even in addition, cuvette minimum gauge, the design of inner cavity also far less likely to occur μm of grade.
This with special flow passage can be added different dyes and dye according to demand with Demand Design for these reasons
Expect the corrector strip of concentration.
Utility model content
The purpose of this utility model is to provide a kind of corrector strip that can be used for the calibration of dry type immunity analysis instrument, solves existing
Unstable, the non-uniform problem of dry corrector strip.
In order to achieve the above object, the utility model provides a kind of corrector strip for the calibration of dry type immunity analysis instrument,
The corrector strip is made of upper cover plate and the conjunction of lower cover lid, and sample introduction window and venthole are provided on the upper cover plate, lower cover setting
There is raceway groove, the raceway groove at least one end has accommodating chamber, wherein an accommodating chamber of the raceway groove of lower cover and the sample introduction window of upper cover plate
It is corresponding above and below position when combining installation.
Preferably, the raceway groove is in several continuous U-shapeds.
Preferably, the both ends of raceway groove are equipped with accommodating chamber.
Preferably, the U-shaped is evenly arranged, U-shaped opening is in the same size, and one end of raceway groove is equipped with accommodating chamber.
Preferably, raceway groove is linear, the both ends of raceway groove are equipped with accommodating chamber.
Preferably, the raceway groove forms rectangular array by the hole that several volumes are incremented by successively or successively decrease from left to right, and
Through entire array and both ends accommodating chamber is connected by an axis wire channel.The hole of rectangular array is not limited to rectangle, or oval
Or circle etc..The characteristics of array, has a series of hole, and the volume in hole has the trend for being incremented by or successively decreasing, while having one
Raceway groove can connect this some holes, sample introduction window, waste liquid pool (or being liquid storage pool), may be implemented to add at sample introduction window so primary
Sample can generate signal (the generation liquid that not only and light path related to solution concentration penetrates of signal of varying strength in array area
Body thickness, liquid cross-sectional product size are all related).
Preferably, upper cover plate is not connected to lower cover or one end is connect.
The utility model designs a series of micro flow channels (i.e. raceway groove) in lower cover plate surface, on upper cover plate punching conduct into
Sample window and venthole.Upper and lower cover plates is become into the corrector strip that can be used for calibrating after being bonded or bonding or buckle is handled.In runner
Sample-adding window at be added liquid dyes, drive through capillary action, liquid dyes oozed under occurring and to leading portion migrate, at 1 point
In clock, dyestuff can submerge entire runner and reach equilibrium state.The shape and processing thickness of runner determine open ended liquid
Volume (flow area * flow channel depths), 15 μ L samples, same runner processing can about be accommodated by such as processing the runner of 50 μ m-thicks
1mm thickness can then accommodate 300 μ L samples.In addition, gate thickness is deepened, corresponding optical signalling can also change, root
According to testing principle difference, signal can rise (transmitting or reflection light detection) or decline and (absorb light detection).
According to equipment evaluation content to be checked, runner has different shapes and combination, generally comprise snakelike, rectangular array, it is linear,
Hole etc..
The advantages of the utility model：
1. corrector strip described in the utility model can according to equipment Alignment require sample-adding window at be added specified amount, have
The particular dye of different excitation-emission spectrum realizes the optical module detection under the spectral conditions, while according to detection signal
Difference between standard value calibrates light source detection module.Such as visible detection module is detected and is calibrated, can be added
Add Conventional dye such as Ponceaux etc.；Into when assessment, specific fluorescent dye should then added to fluoroscopic examination module.
2. corrector strip described in the utility model devises different runners, when being applied in combination, these runners are in equipment
When horizontal direction or directions of rays deviate, detected signal also can accordingly deviate standard value, can carry out equipment accordingly
The detection and calibration of horizontal direction and directions of rays.
3. corrector strip trial described in the utility model designs different size of hole on same lower cover, these holes
It communicates with each other, dye strength is consistent, and hole volume is in incremental or decline trend.Result, it is believed that same sample can be in different holes
Place generates the signal of varying strength, and the difference between signal should there are consistency with hole volume difference, such as there is significant difference,
Should be only related to the resolution ratio of light source detection module, the ratio of high lower value signals difference and hole volume difference is used as equipment
The evaluation criteria of resolution ratio accordingly can also calibrate equipment.
4. corrector strip described in the utility model can be used for the optical detecting module of assessment equipment, since its is disposable
The characteristics of, can avoid very well dyestuff from repeatedly excite in occur fluorescent quenching, decaying the problems such as.Simultaneously as solution
The characteristics of homogeneity, can avoid the dry plate non-uniform negative effect brought to equipment Alignment when inkjet printing very well.
5. corrector strip described in the utility model can be according to the automatic dilution precision of device requirement assessment equipment.
6. corrector strip described in the utility model can be according to the automatic sample precision of device requirement assessment equipment.
Description of the drawings
Fig. 1 is a kind of corrector strip (corrector strip of embodiment 1 for the calibration of dry type immunity analysis instrument of the utility model
1) structural schematic diagram.
Fig. 2 is a kind of corrector strip (corrector strip of embodiment 2 for the calibration of dry type immunity analysis instrument of the utility model
2) structural schematic diagram.
Fig. 3 is a kind of corrector strip (corrector strip of embodiment 3 for the calibration of dry type immunity analysis instrument of the utility model
3) structural schematic diagram.
Fig. 4 is a kind of corrector strip (corrector strip of embodiment 4 for the calibration of dry type immunity analysis instrument of the utility model
4) structural schematic diagram.
Specific implementation mode
The technical solution of the utility model is described further below in conjunction with drawings and examples.
Embodiment 1
A kind of corrector strip for the calibration of dry type immunity analysis instrument by upper cover plate 10 and the lid of lower cover 20 as shown in Figure 1, closed
It constitutes, is provided with sample introduction window 11 and venthole 12 on the upper cover plate 10, is preferably placed at the both ends of upper cover plate, the lower cover 20 setting
There is raceway groove 21, which is equipped with accommodating chamber 211, wherein an accommodating chamber of raceway groove and the sample introduction window 11 of upper cover plate exist
Position is corresponding up and down when combination installation, another accommodating chamber is corresponding with 12 upper and lower position of venthole.If the raceway groove is in involvement
Continuous U-shaped, the U-shaped opening it is in the same size or inconsistent.
With PMMA (polymethyl methacrylate) for base material, processing prepares the above, the lower lid, and the working depth on lower cover
For the raceway groove of 50 μm~1mm, on upper cover plate punching prepare sample introduction window and venthole, then upper and lower cover plates is adhesively fixed
Or buckle is fixed, that is, obtains corrector strip 1.
Corrector strip 1 prepared by the embodiment 1 is used for detection module standardised assessment.It is loaded window on the right side of corrector strip
Standardized dyestuff (such as Ponceaux, bromobenzene gentian violet) is added in place, and the runner of 50 μ m-thicks can about accommodate 15 μ L samples, same to flow
Road processing 1mm thickness can then accommodate 300 μ L samples, liquid flowing reach balance after (about 1 minute), be placed in it is standardized can
Signal acquisition is carried out on light-exposed detection module.Then, corrector strip is placed on the optical detecting module of equipment to be checked and carries out signal
Acquisition, whether the optical detecting module that equipment to be checked is assessed according to the up-regulation of signal or decline reaches expected requirement, and treats
The component of inspection equipment carries out debugging until the collected signal of equipment to be checked is consistent with the signal that standardized optical modules acquire.
The signal that the corrector strip is obtained is relatively uniform smooth, does not occur often occurring when the dry plate calibration such as inkjet printing
Signal burr (i.e. ink-jet uneven caused by higher background noise), with relatively good excellent when normalized signal
Gesture.
Embodiment 2
A kind of corrector strip for the calibration of dry type immunity analysis instrument by upper cover plate 10 and the lid of lower cover 20 as shown in Fig. 2, closed
It constitutes, is provided with sample introduction window 11 and venthole 12 on the upper cover plate 10, is preferably placed at the both ends of upper cover plate, the lower cover 20 setting
There is raceway groove 21, which is equipped with accommodating chamber 211, wherein an accommodating chamber of raceway groove and the sample introduction window 11 of upper cover plate exist
Position is corresponding up and down when combination installation, another accommodating chamber is corresponding with 12 upper and lower position of venthole.The raceway groove is linear,
Uniformly it is laid with (channel width is consistent).
Using PDMS as base material, processing prepares the above, the lower lid, and working depth is the ditch of 50 μm~1mm on lower cover
Road, on upper cover plate punching prepare sample introduction window 11 and venthole 12, then upper and lower cover plates is adhesively fixed or is buckled is solid
It is fixed, that is, obtain corrector strip 2.
Corrector strip prepared by embodiment 1 and embodiment 2 is applied in combination, for commenting equipment level and vertical direction
Estimate.It is loaded at window on the right side of corrector strip 1 and corrector strip 2 and a certain amount of standardized solution is added, after ready to balance, be put into be checked
In the fluoroscopic examination module of equipment, right-to-left scans and generates signal, if corrector strip 1 and 2 signals have unilateral side to raise up simultaneously
Or the case where lowering, you can judge that the equipment level direction calibration is problematic；Only signal deviation occurs for corrector strip 2, then illustrates
Directions of rays is not calibrated complete.Since the corrector strip belongs to medical disposable material, the fluorescent material of addition, which only excites 1 time, to be thrown
It abandons, avoids fluorescent material because exciting unstable disadvantage repeatedly well.
As it can be seen that using corrector strip 1 and corrector strip 2 simultaneously, the calibration of assessment equipment horizontal direction and directions of rays can be used to.
Embodiment 3
A kind of corrector strip for the calibration of dry type immunity analysis instrument by upper cover plate 10 and the lid of lower cover 20 as shown in figure 3, closed
It constitutes, is provided with sample introduction window 11 and venthole 12 on the upper cover plate 10, is preferably placed at the both ends of upper cover plate, the lower cover 20 setting
There is raceway groove 21, which is equipped with accommodating chamber 211, wherein an accommodating chamber of raceway groove and the sample introduction window 11 of upper cover plate exist
Position is corresponding up and down when combination installation, another accommodating chamber is corresponding with 12 upper and lower position of venthole.The raceway groove is by one under
First raceway groove 212 of cover board longitudinal direction, and, several with the first channel vertical and communicate setting the second raceway groove 213 constitute, should
First raceway groove 212 is linear, and 213 uniform intervals of the second raceway groove setting, every second raceway groove 213 is divided by the first raceway groove 212
Symmetrical two sections are divided into, is located at the both sides of the first raceway groove 212, and length is in incremental or decline trend.Preferably, second raceway groove
Length is successively decreased from close accommodating chamber corresponding with sample-adding window to other side accommodating chamber (corresponding with well) direction length.
Using BF33 high-boron-silicon glass as base material, processing prepare the above, the lower lid, and on lower cover working depth be 50 μm~
The raceway groove of 1mm, on upper cover plate punching prepare sample introduction window 11 and venthole 12, then upper and lower cover plates is adhesively fixed or
Buckle is fixed, that is, obtains corrector strip 3.
Corrector strip 3 is used for the assessment to can detect range and resolution ratio.On the right side of corrector strip 3, sample-adding window is added specific
Dyestuff can be used for assessing and calibrating equipment is immunized in dry type resolution ratio and detection range, from left-hand such as Ponceaux or fluorescein
Right signal should increase successively, with the resolution ratio of assessment equipment and can be examined according to signal amplification difference and lowest position signal value
Survey range, and ensure that equipment is reaching certain response while having preferable resolution ratio, to reach to not on the same stage equipment into
Row effectively standardized requirement.
Embodiment 4
A kind of corrector strip for the calibration of dry type immunity analysis instrument by upper cover plate 10 and the lid of lower cover 20 as shown in figure 4, closed
It constitutes, is provided with sample introduction window 11 and venthole 12 on the upper cover plate 10, is preferably placed at the both ends of upper cover plate, the lower cover 20 setting
Have raceway groove 21, which is equipped with accommodating chamber 211, the accommodating chamber and the sample introduction window 11 of upper cover plate combine install when position on
Lower correspondence.The raceway groove is in several continuous U-shapeds, in the same size, the uniformly laying of the U-shaped opening.
Using quartz glass as base material, processing prepares the above, the lower lid, and the ditch that working depth is 200 μm on lower cover
Road, on upper cover plate punching prepare sample introduction window 11 and venthole 12, then upper and lower cover plates is adhesively fixed or is buckled is solid
It is fixed, that is, obtain corrector strip 4.
Corrector strip 4 is for the assessment to automatic loading precision.The raceway groove of corrector strip 4 need to accurately control mismachining tolerance.Such as set
Timing can accommodate the sample of 70 μ L, can be used for assessing the accurate of the loading within the 60 μ L of dry type immunity analysis instrument of automatic loading
Property, the length of raceway groove is filled according to sample and the collected continuous peak area of signal on standardized detection module, can used
To judge the equipment loading precision.
Embodiment 5
Corrector strip 1 prepared by embodiment 1 can also be used for the assessment to automatic dilution precision.Using 100 × (i.e. 100 times,
If sample is 1g/mL, 100 × mother liquor is exactly 100g/mL；If sample is 0.01%, 100 × mother liquor is exactly 1%；As sample is
1mmol/L, 100 × mother liquor are exactly 100mmol/L) standardization mother liquor, by equipment automatic dilution at 1 × sample (that is, dilution
100 times), it is added in the sample-adding window on 1 right side of corrector strip, corresponding signal is acquired on optical detecting module, with 1 × normalization solution
Produced signal fusing, it may be determined that the precision of automatic dilution.
Embodiment 6
Corrector strip described in the utility model can be according to the automatic sample precision of device requirement assessment equipment.
Using 1 × solution after standardization, sample is dripped using equipment automatic sampling to be checked again and arrives corrector strip (by being loaded window)
On, normalised detection device detection collects optical detector signal, by the optical detector signal being collected into and standardized 1
The signal of the normalised detection device detection of × solution relatively judges the precision of loading.
Although the content of the utility model is discussed in detail by above preferred embodiment, but it should be appreciated that on
The description stated is not considered as limitations of the present invention.After those skilled in the art have read the above, for
A variety of modifications and substitutions of the utility model all will be apparent.Therefore, the scope of protection of the utility model should be by appended
Claim limit.
Claims (9)
1. a kind of corrector strip for the calibration of dry type immunity analysis instrument, which is characterized in that the corrector strip is by upper cover plate and lower cover
Lid, which closes, to be constituted, and is provided with sample introduction window and venthole on the upper cover plate, which is provided with raceway groove, and the raceway groove at least one end has
Accommodating chamber a, wherein accommodating chamber of the raceway groove of lower cover is corresponded to when combining installation above and below position with the sample introduction window of upper cover plate.
2. the corrector strip for the calibration of dry type immunity analysis instrument as described in claim 1, which is characterized in that the raceway groove is in
Snakelike, rectangular array, linear, hole.
3. the corrector strip for the calibration of dry type immunity analysis instrument as described in claim 1, which is characterized in that the raceway groove is in
Several continuous U-shapeds.
4. the corrector strip for the calibration of dry type immunity analysis instrument as claimed in claim 3, which is characterized in that the both ends of raceway groove are equal
Equipped with accommodating chamber.
5. the corrector strip for the calibration of dry type immunity analysis instrument as claimed in claim 3, which is characterized in that the U-shaped is equal
Even arrangement, U-shaped opening is in the same size, and one end of raceway groove is equipped with accommodating chamber.
6. the corrector strip for the calibration of dry type immunity analysis instrument as described in claim 1, which is characterized in that raceway groove is linear
The both ends of shape, raceway groove are equipped with accommodating chamber.
7. the corrector strip for the calibration of dry type immunity analysis instrument as claimed in claim 1 or 2, which is characterized in that the ditch
The both ends in road are equipped with accommodating chamber, and the raceway groove forms square by the hole that several volumes are incremented by successively or successively decrease from left to right
Array, the hole select rectangle, ellipse or circle, and run through entire rectangular array by an axis wire channel and connect the receiving at both ends
Chamber.
8. the corrector strip for the calibration of dry type immunity analysis instrument as described in claim 1, which is characterized in that upper cover plate and lower cover
Plate is not connected to or one end connection.
9. the corrector strip for the calibration of dry type immunity analysis instrument as described in claim 1, which is characterized in that upper cover plate and lower cover
Plate is closed through bonding or buckling lid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721463906.2U CN208043837U (en) | 2017-11-06 | 2017-11-06 | A kind of corrector strip for the calibration of dry type immunity analysis instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721463906.2U CN208043837U (en) | 2017-11-06 | 2017-11-06 | A kind of corrector strip for the calibration of dry type immunity analysis instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208043837U true CN208043837U (en) | 2018-11-02 |
Family
ID=63957765
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721463906.2U Active CN208043837U (en) | 2017-11-06 | 2017-11-06 | A kind of corrector strip for the calibration of dry type immunity analysis instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208043837U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020155241A1 (en) * | 2019-02-01 | 2020-08-06 | 深圳市金准生物医学工程有限公司 | Fluorescence standard card for calibration and quality control of fluorescence immunoassay analyzer and test method |
-
2017
- 2017-11-06 CN CN201721463906.2U patent/CN208043837U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020155241A1 (en) * | 2019-02-01 | 2020-08-06 | 深圳市金准生物医学工程有限公司 | Fluorescence standard card for calibration and quality control of fluorescence immunoassay analyzer and test method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2648147C (en) | System and method for determining the concentration of an analyte in a liquid sample | |
| US8679426B2 (en) | Microscope accessory and microplate apparatus for measuring phosphorescence and cellular oxygen consumption | |
| US10807087B2 (en) | Lateral flow device, assay device and kit and method for analyzing a fluid sample | |
| WO2015070701A1 (en) | Test strip card | |
| US20060057708A1 (en) | Testing plate | |
| KR20190059307A (en) | Analytical test equipment | |
| ES2388005T3 (en) | Internal test standardization procedure | |
| CN107664695A (en) | A kind of corrector strip and calibration method for the calibration of dry type immunity analysis instrument | |
| RU2519505C2 (en) | Sensor device for target substance identification | |
| CN105510577A (en) | Method for rapidly and quantitatively detecting multiple analytes in blood by adopting multi-point calibration | |
| Wu et al. | Widefield frequency domain fluorescence lifetime imaging microscopy (FD-FLIM) for accurate measurement of oxygen gradients within microfluidic devices | |
| CN208043837U (en) | A kind of corrector strip for the calibration of dry type immunity analysis instrument | |
| DE102010038431A1 (en) | Measuring cassette and measuring device for the detection of target molecules in a liquid sample by measuring fluorescence emission after excitation in the evanescent field | |
| US8865454B2 (en) | Flow through system, flow through device and a method of performing a test | |
| JP4406587B2 (en) | Inspection plate | |
| US20040069639A1 (en) | Resin chip | |
| CN106605144B (en) | Method for measuring and system are solidified for carrying out point-of-care by light detection | |
| KR102626214B1 (en) | A Disposable Diagnostic Cartridge | |
| KR102224374B1 (en) | Reference cassette for fluorescence type diagnostic apparatus for immunoassay | |
| CN114931987B (en) | Centrifugal micro-fluidic chip and method for synchronously detecting multiple ions in soil | |
| EP3484686B1 (en) | Sample vessel having opaque and translucent portions, and sample analyzer system with such a sample vessel | |
| JP2008197053A (en) | Flow-channel formation chip for biological sample, and manufacturing method for the flow-channel formation chip for biological sample | |
| DE112022002144T5 (en) | FLEX FILM, MICROFLUIDIC CARTRIDGE AND MICROFLUIDIC ARRANGEMENT | |
| KR20230058321A (en) | Vertical Flow Analysis Apparatus and Method for Determination of Hemoglobin Concentration | |
| CN108226552A (en) | A kind of full-automatic high-throughput batch processing instrument for measuring micro-example optical characteristics |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |