CN111766387A - Preparation method of quality control card and quality control card - Google Patents
Preparation method of quality control card and quality control card Download PDFInfo
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- CN111766387A CN111766387A CN202010663605.4A CN202010663605A CN111766387A CN 111766387 A CN111766387 A CN 111766387A CN 202010663605 A CN202010663605 A CN 202010663605A CN 111766387 A CN111766387 A CN 111766387A
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Abstract
The invention provides a preparation method of a quality control card and the quality control card, wherein the method comprises the following steps: dissolving sodium salt to form sodium salt solution; trehalose, bovine serum albumin and glycerol are put in a solvent to form a protective solution; respectively adding a protective solution into the sodium salt solution to dilute the sodium salt solution to obtain disodium salt standard solutions with different concentrations; forming reference scribes on the three substrate films respectively by using the disodium salt standard solution with the first sodium salt concentration, forming first scribes on the first substrate film by using the disodium salt standard solution with the first sodium salt concentration, forming second scribes on the second substrate film by using the disodium salt standard solution with the second sodium salt concentration, and forming third scribes on the third substrate film by using the disodium salt standard solution with the third sodium salt concentration; the first sodium salt concentration is different from the second sodium salt concentration, and the first sodium salt concentration is different from the third sodium salt concentration; and drying to obtain the quality control card. The quality control card prepared by the method has good stability, is not easily influenced by the environment, and can effectively control the quality of instruments.
Description
Technical Field
The invention relates to the field of quality control cards, in particular to a preparation method of a quality control card and the quality control card.
Background
The fluorescence immunochromatography is to mark fluorescent substances which do not influence the activity of antigen-antibody on the antibody, combine with corresponding antigen, make the tracer send out fluorescent signal under the action of the signal source of the fluorescence immunoassay analyzer, and then scan and draw the signal intensity curve of the tracer by the sensor to carry out data analysis, thereby obtaining the content of the object to be detected. The fluorescence immunochromatography does not need complex operation flow, professional technicians and expensive equipment, has the advantages of short detection time, quantifiability, high sensitivity, good stability and the like, and is widely applied to the fields of medical treatment, environment, food and the like.
The fluorescence immunoassay analyzer is susceptible to the influence of factors such as signal source intensity, sensor stability, electronic element attenuation and test environment, so that the test result of the analyzer is inaccurate, the difference between batch test results and batch test results is large, even the test results of the same analyzer in different time periods are inconsistent, and a quality control card for production, debugging and self-checking of the analyzer needs to be prepared.
At present, related manufacturers generally adopt fluorescent microspheres, quantum dots or isothiocyanic acid and other substances as a quality control card, but fluorescent substances or fluorescent quantum dots are easily influenced by the surrounding environment, the stability is difficult to guarantee, so that the standard making and long-term calibration tracing are difficult, a tested fluorescent card strip cannot be stored for a long time, a tested fluorescent card strip needs to be reused every time equipment is detected, the difference among different batches is large, and accurate debugging on an instrument is difficult.
Disclosure of Invention
In view of this, the invention provides a quality control card and a preparation method thereof, which are used for solving the problems that the existing quality control card is poor in stability, not easy to store for a long time, easy to be influenced by external environment and not easy to perform self-checking on instruments.
In order to solve the technical problems, the invention adopts the following technical scheme:
in a first aspect, a method for preparing a quality control card according to an embodiment of the present invention includes:
dissolving sodium salt to form sodium salt solution;
placing trehalose, bovine serum albumin and glycerol in a solvent to form a protective solution;
adding the protective solution to the sodium salt solution respectively to dilute the sodium salt solution to obtain a disodium salt standard solution with a first sodium salt concentration, a second sodium salt concentration and a third sodium salt concentration;
respectively scribing reference scribing lines on the three substrate films by using disodium salt standard solution with first sodium salt concentration, scribing first scribing lines on the first substrate film by using disodium salt standard solution with first sodium salt concentration, scribing second scribing lines on the second substrate film by using disodium salt standard solution with second sodium salt concentration, and scribing third scribing lines on the third substrate film by using disodium salt standard solution with third sodium salt concentration;
wherein the two scribe lines on each of the substrate films are parallel, the first sodium salt concentration is different from the second sodium salt concentration, and the first sodium salt concentration is different from the third sodium salt concentration; after drying, two marking lines on each substrate film respectively form a fluorescent strip to obtain the quality control card;
the sodium salt is bis [4- (N-ethyl-N-3-sulfonic acid benzyl) aminophenyl ] -2-sulfonic acid tolyl disodium salt.
Wherein, placing trehalose, bovine serum albumin and glycerol in a solvent to form a protective solution, comprising:
dissolving trehalose, bovine serum albumin and glycerol in pure water, and uniformly mixing to form the protective solution.
Wherein the concentration of sodium salt in the disodium salt standard solution is 1-100 mg/L.
Wherein, in the protective solution:
the concentration of trehalose is 1-10%;
the concentration of bovine serum albumin is 1-10%;
the concentration of glycerol is 0.1-5%.
Wherein, in the protective solution:
the concentration of trehalose is 5%;
the concentration of bovine serum albumin is 4%;
the concentration of glycerol was 1%.
In a second aspect, a quality control card according to an embodiment of the present invention includes:
the fluorescent film comprises three substrate films, wherein each substrate film is respectively provided with a reference fluorescent strip, a first fluorescent strip is formed on the first substrate film, a second fluorescent strip is formed on the second substrate film, and a third fluorescent strip is formed on the third substrate film;
wherein the two fluorescence bands on each substrate film are parallel, and the reference fluorescence band, the first fluorescence band, the second fluorescence band and the third fluorescence band respectively have sodium salt, trehalose, bovine serum albumin and glycerol therein; the reference fluorescence band is the same as the first fluorescence band, the sodium salt content in the first fluorescence band is different from that in the second fluorescence band, and the sodium salt content in the first fluorescence band is different from that in the third fluorescence band;
the sodium salt is bis [4- (N-ethyl-N-3-sulfonic acid benzyl) aminophenyl ] -2-sulfonic acid tolyl disodium salt.
Wherein the trehalose content in each fluorescent strip is 1-10 parts by weight;
the content of bovine serum albumin is 1-10 parts by weight;
the content of glycerin is 0.1-5 weight parts.
Wherein the trehalose content in each fluorescent strip is 5 parts by weight;
the bovine serum albumin content was 4 parts by weight;
the content of glycerin was 1 part by weight.
Wherein the sodium salt content in the second fluorescent band is different from that in the third fluorescent band.
Wherein, still include:
the bottom plate, be equipped with the recess on the bottom plate, the substrate membrane is established in the recess.
The technical scheme of the invention has the following beneficial effects:
according to the preparation method of the quality control card, the sodium salt is dissolved to form a sodium salt solution; placing trehalose, bovine serum albumin and glycerol in a solvent to form a protective solution; adding the protective solution to the sodium salt solution respectively to dilute the sodium salt solution to obtain a disodium salt standard solution with a first sodium salt concentration, a second sodium salt concentration and a third sodium salt concentration; respectively scribing reference scribing lines on the three substrate films by using disodium salt standard solution with first sodium salt concentration, scribing first scribing lines on the first substrate film by using disodium salt standard solution with first sodium salt concentration, scribing second scribing lines on the second substrate film by using disodium salt standard solution with second sodium salt concentration, and scribing third scribing lines on the third substrate film by using disodium salt standard solution with third sodium salt concentration; wherein the two scribe lines on each of the substrate films are parallel, the first sodium salt concentration is different from the second sodium salt concentration, and the first sodium salt concentration is different from the third sodium salt concentration; after drying, two marking lines on each substrate film respectively form a fluorescent strip to obtain the quality control card; the sodium salt is bis [4- (N-ethyl-N-3-sulfonic acid benzyl) aminophenyl ] -2-sulfonic acid tolyl disodium salt. The quality control card prepared by the method has the advantages of light resistance, heat resistance, good stability and long-term storage, can be used for self-checking of a fluorescence analyzer by adopting a reagent which is not easily influenced by excitation frequency tests, is not easily influenced by factors such as environment and the like, and the test result is not gradually attenuated along with the time, so that the validity of the fluorescence quality control card is ensured, the fluorescence analyzer is favorably subjected to effective quality control, and the use is convenient and rapid.
Drawings
FIG. 1 is a schematic flow chart of a preparation method of an embodiment of the present invention;
FIG. 2 is a schematic diagram showing the distribution of fluorescent stripes in the quality control card according to the embodiment of the present invention;
FIG. 3 is a diagram of a quality control card according to an embodiment of the present invention.
Reference numerals
A substrate film 10; a first fluorescent strip 11; a second fluorescent strip 12; a third fluorescent strip 13; a reference fluorescence band 14;
a base plate 20.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the drawings of the embodiments of the present invention. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the invention, are within the scope of the invention.
The method for preparing the quality control card according to the embodiment of the present invention is described in detail below.
As shown in fig. 1, the method for preparing a quality control card according to an embodiment of the present invention includes:
step S1, dissolving sodium salt to form sodium salt solution;
step S2, placing trehalose, bovine serum albumin and glycerol in a solvent to form a protective solution;
step S3, respectively adding a protective solution into the sodium salt solution to dilute the sodium salt solution to obtain a disodium salt standard solution with a first sodium salt concentration, a second sodium salt concentration and a third sodium salt concentration;
step S4, respectively scribing reference scribing lines on the three substrate films by using the disodium salt standard solution with the first sodium salt concentration, scribing first scribing lines on the first substrate film by using the disodium salt standard solution with the first sodium salt concentration, scribing second scribing lines on the second substrate film by using the disodium salt standard solution with the second sodium salt concentration, and scribing third scribing lines on the third substrate film by using the disodium salt standard solution with the third sodium salt concentration;
wherein the two scribe lines on each of the substrate films are parallel, the first sodium salt concentration is different from the second sodium salt concentration, and the first sodium salt concentration is different from the third sodium salt concentration;
and step S5, respectively forming fluorescent strips on the two scribed lines on each substrate film after drying, and obtaining the quality control card. Wherein the sodium salt is bis [4- (N-ethyl-N-3-sulfonic acid benzyl) aminophenyl ] -2-sulfonic acid tolyl disodium salt.
That is, during the preparation process, the sodium salt can be dissolved to form a sodium salt solution, the trehalose, the bovine serum albumin and the glycerol are placed in a solvent to form a protective solution, the solvent can be deionized water or high-purity water, diluting the sodium salt solution by the protective solution, respectively adding the protective solution into the sodium salt solution to dilute the sodium salt solution to obtain disodium salt standard solution with a plurality of sodium salt concentrations, a disodium salt standard solution having a first sodium salt concentration, a second sodium salt concentration and a third sodium salt concentration can be obtained, the concentration of sodium salt in the disodium salt standard solution can be 1-100mg/L, the concentration of sodium salt in the disodium salt standard solution can be three concentrations, such as 50mg/L, 5mg/L and 1mg/L, the first sodium salt concentration can be 5mg/L, the second sodium salt concentration can be 50mg/L and the third sodium salt concentration can be 1 mg/L. Sodium salt can be protected through trehalose, bovine serum albumin and glycerine for the sodium salt is stable, is difficult for receiving external influence, and the substrate membrane can be nitrocellulose membrane or polyvinylidene fluoride (PVDF) membrane, and the substrate membrane can be established on the bottom plate, and the bottom plate can be polyvinyl chloride (PVC) board or polyvinylpyrrolidone (PVP) board.
The second sodium salt concentration and the third sodium salt concentration may be different, for example, the first sodium salt concentration may be 5mg/L, the second sodium salt concentration may be 50mg/L, and the third sodium salt concentration may be 1 mg/L. The reference scribing lines can be formed by respectively scribing on the three substrate films by using a disodium salt standard solution with a first sodium salt concentration, then scribing on the first substrate film by using the disodium salt standard solution with the first sodium salt concentration to form first scribing lines, scribing on the second substrate film by using a disodium salt standard solution with a second sodium salt concentration to form second scribing lines, and scribing on the third substrate film by using a disodium salt standard solution with a third sodium salt concentration to form third scribing lines; wherein the sodium salt is bis [4- (N-ethyl-N-3-sulfonic acid benzyl) aminophenyl ] -2-sulfonic acid tolyl disodium salt, the first sodium salt concentration can be 5mg/L, the second sodium salt concentration can be 50mg/L, and the third sodium salt concentration can be 1mg/L, two lines on each substrate film are parallel, the first sodium salt concentration is different from the second sodium salt concentration, and the first sodium salt concentration is different from the third sodium salt concentration; the reference scribe line on the first substrate film is parallel to the first scribe line, the reference scribe line on the second substrate film is parallel to the second scribe line, and the reference scribe line on the third substrate film is parallel to the third scribe line.
And (3) scribing by using a scribing instrument, drying the scribed substrate film in a drying oven at 37 ℃ for 5 hours, and respectively forming fluorescent strips by two scribed lines after drying to obtain the quality control card. After drying, as shown in fig. 2 and 3, the reference scribe lines on the substrate films form reference fluorescent stripes, the first scribe lines on the first substrate film form first fluorescent stripes, the second scribe lines on the second substrate film form second fluorescent stripes, and the third scribe lines on the third substrate film form third fluorescent stripes; the reference fluorescence band on the first substrate film is parallel to the first fluorescence band, the reference fluorescence band on the second substrate film is parallel to the second fluorescence band, and the reference fluorescence band on the third substrate film is parallel to the third fluorescence band.
The bottom plate with the fluorescent strips can be cut, packaged and pressed according to requirements, the dried bottom plate can be respectively cut into small strips with the width (3.90 +/-0.01) mm and placed into a matched card shell, and quality control cards with different concentrations in different heights can be respectively taken and packaged in a self-sealing bag, so that the complete quality control card set is formed, and the fluorescent immunoassay analyzer can be used for testing. The quality control card prepared by the method can protect sodium salt through trehalose, bovine serum albumin and glycerol, so that the sodium salt is stable and is not easily influenced by the outside, the quality control card can resist light and heat, has good stability and can be stored for a long time, the fluorescent quality control card is prepared by adopting a reagent which is not easily influenced by the excitation frequency test, the fluorescent quality control card can be used for self-checking of a fluorescence analyzer and is not easily influenced by factors such as the environment, the test result is not attenuated gradually along with the time, the validity of the fluorescent quality control card is ensured, the fluorescent quality control card is favorable for carrying out effective quality control on the fluorescence analyzer, and the use is convenient and rapid.
In some embodiments of the invention, dissolving the sodium salt forms a sodium salt solution comprising:
bis [4- (N-ethyl-N-3-sulfonic acid benzyl) aminophenyl ] -2-sulfonic acid tolyl disodium salt is dissolved in dimethyl sulfoxide to form a sodium salt solution, for example, 0.01g of bis [4- (N-ethyl-N-3-sulfonic acid benzyl) aminophenyl ] -2-sulfonic acid tolyl disodium salt is weighed and placed in a 10ml volumetric flask, dissolved with dimethyl sulfoxide to a constant volume, and sufficiently and uniformly mixed to obtain a sodium salt solution (mother liquor) with a concentration of 1000 mg/L.
In some embodiments of the invention, trehalose, bovine serum albumin and glycerol are placed in a solvent to form a protective solution comprising:
dissolving trehalose, bovine serum albumin and glycerol in pure water, and uniformly mixing to form the protective solution. For example, trehalose, bovine serum albumin and glycerol may be dissolved in pure water to obtain a protective solution, and different amounts of protective solutions may be added to a mother liquor having a sodium salt concentration of 1000mg/L to dilute the mother liquor, respectively, to obtain disodium salt standard solutions having sodium salt concentrations of 50mg/L, 5mg/L and 1 mg/L.
The bis [4- (N-ethyl-N-3-sulfobenzyl) aminophenyl ] -2-sulfomethylphenyl disodium salt is a substance prepared by condensing and oxidizing benzaldehyde o-sulfonic acid and N-ethyl-N- (3-sulfobenzyl) -aniline, is light-resistant and heat-resistant, is stable to citric acid, tartaric acid and alkali, and is not easily influenced by factors such as environment and the like.
Optionally, in the protective solution, the concentration of trehalose is 1-10%, the concentration of bovine serum albumin is 1-10%, and the concentration of glycerol is 0.1-5%. For example, in the protective solution, deionized water can be used as a solvent, the concentration of trehalose is 5%, the concentration of bovine serum albumin is 4%, and the concentration of glycerol is 1%; according to some embodiments, deionized water can be used as a solvent in the protective solution, the concentration of trehalose is 1%, the concentration of bovine serum albumin is 10%, and the concentration of glycerol is 0.1%; in some embodiments, the protecting solution may be prepared by using deionized water as solvent, and has trehalose concentration of 10%, bovine serum albumin concentration of 1%, and glycerin concentration of 5%, and has good stability.
Similar substances can be selected according to different wavelengths, for example, a fluorescence analyzer with the wavelength of about 430nm can use 1- (4-sulfophenyl) -4- (4-sulfophenylazo) -5-pyrazolone-3-carboxylic acid trisodium salt as a sodium salt, and a fluorescence analyzer with the wavelength of about 500nm can use 6-hydroxy-5- (2-methoxy-4-sulfoacid-5-methylphenyl) azonaphthalene-2-sulfoacid disodium salt as a sodium salt, so that the problem that the fluorescence signal value tested by an instrument is possibly not matched with the fluorescence signal value tested by a test strip due to the fact that a quality control card and a fluorescent substance tested by the immune test strip are not the same in wavelength, and the instrument cannot be effectively controlled in quality is solved.
The embodiment of the invention provides a quality control card, and the quality control card in the embodiment of the invention can be prepared by the preparation method in the embodiment.
As shown in fig. 2 and 3, the quality control card in the embodiment of the present invention includes: three substrate films 10, wherein each substrate film 10 is respectively provided with a reference fluorescent strip 14, a first substrate film 10 is provided with a first fluorescent strip 11, a second substrate film 10 is provided with a second fluorescent strip 12, and a third substrate film 10 is provided with a third fluorescent strip 13; wherein, the two fluorescence bands on each substrate film 10 are parallel, and the reference fluorescence band 14, the first fluorescence band 11, the second fluorescence band 12 and the third fluorescence band 13 respectively have sodium salt, trehalose, bovine serum albumin and glycerol; the reference fluorescence band 14 is the same as the first fluorescence band 11, the sodium salt content in the first fluorescence band 11 is different from that in the second fluorescence band 12, and the sodium salt content in the first fluorescence band 11 is different from that in the third fluorescence band 13; the sodium salt is bis [4- (N-ethyl-N-3-sulfonic acid benzyl) aminophenyl ] -2-sulfonic acid tolyl disodium salt.
That is, the quality control card includes three substrate films 10, a reference fluorescence stripe 14 and a first fluorescence stripe 11 are formed on the first substrate film 10, the reference fluorescence stripe 14 and the first fluorescence stripe 11 on the first substrate film 10 are parallel, a reference fluorescence stripe 14 and a second fluorescence stripe 12 are formed on the second substrate film 10, the reference fluorescence stripe 14 and the second fluorescence stripe 12 on the second substrate film 10 are parallel, a reference fluorescence stripe 14 and a third fluorescence stripe 13 are formed on the third substrate film 10, and the reference fluorescence stripe 14 and the third fluorescence stripe 13 on the third substrate film 10 are parallel;
wherein, the reference fluorescence band 14, the first fluorescence band 11, the second fluorescence band 12 and the third fluorescence band 13 respectively have sodium salt, trehalose, bovine serum albumin and glycerol; the components and the component contents in the reference fluorescence band 14 and the first fluorescence band 11 may be the same, the sodium salt contents in the first fluorescence band 11 and the second fluorescence band 12 may be different, and the sodium salt contents in the first fluorescence band 11 and the third fluorescence band 13 may be different.
The second fluorescent stripe 12 and the third fluorescent stripe 13 may be different, and the sodium salt content in the second fluorescent stripe 12 and the third fluorescent stripe 13 may be different, for example, the sodium salt content in the first fluorescent stripe 11 may be 5 parts by weight, the sodium salt content in the second fluorescent stripe 12 may be 50 parts by weight, and the sodium salt content in the third fluorescent stripe 13 may be 1 part by weight, which may be reasonably selected according to actual needs.
The sodium salt in the fluorescent strip is stable, the sodium salt can be protected by trehalose, bovine serum albumin and glycerol, the stability of the sodium salt is ensured, the sodium salt is not easily influenced by the outside, the quality control card can resist light and heat, has good stability and can be stored for a long time, the fluorescent quality control card is manufactured by adopting a reagent which is not easily influenced by the excitation frequency test, the fluorescent quality control card can be used for self-checking of a fluorescence analyzer, the test result is not gradually attenuated along with the time, the fluorescent quality control card is beneficial to effective quality control of the fluorescence analyzer, and the use is convenient and rapid.
In the embodiment of the present invention, the trehalose content in each fluorescent strip is 1 to 10 parts by weight, the bovine serum albumin content is 1 to 10 parts by weight, and the glycerol content is 0.1 to 5 parts by weight, for example, the trehalose content in each fluorescent strip is 5 parts by weight, the bovine serum albumin content is 4 parts by weight, and the glycerol content is 1 part by weight, and after the trehalose, the bovine serum albumin, and the glycerol content in each fluorescent strip are also the disodium salt standard solutions with different sodium salt concentrations are scribed, the solvent content of the disodium salt standard solution is removed after the scribing and drying of the disodium salt standard solution.
According to some embodiments, the trehalose is present in an amount of 1 part by weight, the bovine serum albumin is present in an amount of 10 parts by weight, and the glycerol is present in an amount of 0.1 part by weight; in some embodiments, the trehalose content in each fluorescent strip is 10 parts by weight, the bovine serum albumin content is 1 part by weight, and the glycerol content is 5 parts by weight, and the trehalose, bovine serum albumin and glycerol content in each fluorescent strip, that is, the content of the solvent is removed after the disodium salt standard solution with different sodium salt concentrations is scribed, and the stability of the fluorescent strip with the components in the ratio is good.
Alternatively, the substrate film 10 may be a nitrocellulose film or polyvinylidene fluoride. Wherein, the quality control card can also include: the substrate 20, the substrate 20 may be a polyvinyl chloride (PVC) plate or a polyvinyl pyrrolidone (PVP) plate, the substrate 20 may be provided with a groove, and the substrate film 10 may be provided in the groove. The groove is internally provided with a contrast area and a detection area, the positions of the contrast area and the detection area correspond to the positions of a quality control line and a detection line in the fluorescence immunochromatography test paper, and the contrast area and the detection area are both provided with fluorescence strips. In practical application, quality control cards with different fluorescence signal values can be selected to form a quality control card set, the result of each quality control card is tested and recorded in sequence and is compared with a standard curve tested by the same method, and when the error is higher than a certain value, the reliability of the test result of the judging instrument is low.
This is further illustrated by the following specific examples.
Example 1
Dissolving sodium salt to form sodium salt solution, and obtaining mother liquor with the sodium salt concentration of 1000 mg/L; dissolving trehalose, bovine serum albumin and glycerol in pure water, and mixing uniformly to form a protective solution; wherein, in the protective solution: the concentration of trehalose was 1%, the concentration of bovine serum albumin was 5%, and the concentration of glycerol was 3%.
Respectively adding a protective solution into the sodium salt solution to dilute the sodium salt solution to obtain disodium salt standard solutions with a plurality of sodium salt concentrations, wherein the sodium salt is bis [4- (N-ethyl-N-3-sulfobenzyl) aminophenyl ] -2-sulfomethylphenyl disodium salt, for example, the first sodium salt concentration is 5mg/L, the second sodium salt concentration is 50mg/L and the third sodium salt concentration is 1mg/L in the three disodium salt standard solutions;
respectively scribing reference scribing lines on the three substrate films by using disodium salt standard solution with first sodium salt concentration, scribing first scribing lines on the first substrate film by using disodium salt standard solution with first sodium salt concentration, scribing second scribing lines on the second substrate film by using disodium salt standard solution with second sodium salt concentration, and scribing third scribing lines on the third substrate film by using disodium salt standard solution with third sodium salt concentration; the two scribe lines on each substrate film are parallel;
after drying, two marking lines on each substrate film respectively form a fluorescent strip to obtain the quality control card;
after drying, forming a reference fluorescent strip by the reference scribing lines on the substrate films, forming a first fluorescent strip by the first scribing lines on the first substrate film, forming a second fluorescent strip by the second scribing lines on the second substrate film, and forming a third fluorescent strip by the third scribing lines on the third substrate film; the reference fluorescence band on the first substrate film is parallel to the first fluorescence band, the reference fluorescence band on the second substrate film is parallel to the second fluorescence band, and the reference fluorescence band on the third substrate film is parallel to the third fluorescence band.
Example 2
Dissolving sodium salt to form sodium salt solution, and obtaining mother liquor with the sodium salt concentration of 1000 mg/L; dissolving trehalose, bovine serum albumin and glycerol in pure water, and mixing uniformly to form a protective solution; wherein, in the protective solution: the trehalose concentration was 5%, the bovine serum albumin concentration was 1%, and the glycerol concentration was 0.1%.
Respectively adding a protective solution into the sodium salt solution to dilute the sodium salt solution to obtain disodium salt standard solutions with a plurality of sodium salt concentrations, wherein the sodium salt is bis [4- (N-ethyl-N-3-sulfobenzyl) aminophenyl ] -2-sulfomethylphenyl disodium salt, for example, the first sodium salt concentration is 5mg/L, the second sodium salt concentration is 50mg/L and the third sodium salt concentration is 1mg/L in the three disodium salt standard solutions;
respectively scribing reference scribing lines on the three substrate films by using disodium salt standard solution with first sodium salt concentration, scribing first scribing lines on the first substrate film by using disodium salt standard solution with first sodium salt concentration, scribing second scribing lines on the second substrate film by using disodium salt standard solution with second sodium salt concentration, and scribing third scribing lines on the third substrate film by using disodium salt standard solution with third sodium salt concentration; the two scribe lines on each substrate film are parallel;
after drying, two marking lines on each substrate film respectively form a fluorescent strip to obtain the quality control card;
after drying, forming a reference fluorescent strip by the reference scribing lines on the substrate films, forming a first fluorescent strip by the first scribing lines on the first substrate film, forming a second fluorescent strip by the second scribing lines on the second substrate film, and forming a third fluorescent strip by the third scribing lines on the third substrate film; the reference fluorescence band on the first substrate film is parallel to the first fluorescence band, the reference fluorescence band on the second substrate film is parallel to the second fluorescence band, and the reference fluorescence band on the third substrate film is parallel to the third fluorescence band.
Example 3
Dissolving sodium salt to form sodium salt solution, and obtaining mother liquor with the sodium salt concentration of 1000 mg/L; dissolving trehalose, bovine serum albumin and glycerol in pure water, and mixing uniformly to form a protective solution; wherein, in the protective solution: the concentration of trehalose was 10%, the concentration of bovine serum albumin was 10%, and the concentration of glycerol was 5%.
Respectively adding a protective solution into the sodium salt solution to dilute the sodium salt solution to obtain disodium salt standard solutions with a plurality of sodium salt concentrations, wherein the sodium salt is bis [4- (N-ethyl-N-3-sulfobenzyl) aminophenyl ] -2-sulfomethylphenyl disodium salt, for example, the first sodium salt concentration is 5mg/L, the second sodium salt concentration is 50mg/L and the third sodium salt concentration is 1mg/L in the three disodium salt standard solutions;
respectively scribing reference scribing lines on the three substrate films by using disodium salt standard solution with first sodium salt concentration, scribing first scribing lines on the first substrate film by using disodium salt standard solution with first sodium salt concentration, scribing second scribing lines on the second substrate film by using disodium salt standard solution with second sodium salt concentration, and scribing third scribing lines on the third substrate film by using disodium salt standard solution with third sodium salt concentration; the two scribe lines on each substrate film are parallel;
after drying, two marking lines on each substrate film respectively form a fluorescent strip to obtain the quality control card;
after drying, forming a reference fluorescent strip by the reference scribing lines on the substrate films, forming a first fluorescent strip by the first scribing lines on the first substrate film, forming a second fluorescent strip by the second scribing lines on the second substrate film, and forming a third fluorescent strip by the third scribing lines on the third substrate film; the reference fluorescence band on the first substrate film is parallel to the first fluorescence band, the reference fluorescence band on the second substrate film is parallel to the second fluorescence band, and the reference fluorescence band on the third substrate film is parallel to the third fluorescence band.
Example 4
Dissolving sodium salt to form sodium salt solution, and obtaining mother liquor with the sodium salt concentration of 1000 mg/L; dissolving trehalose, bovine serum albumin and glycerol in pure water, and mixing uniformly to form a protective solution; wherein, in the protective solution: the concentration of trehalose was 1%, the concentration of bovine serum albumin was 6%, and the concentration of glycerol was 4%.
Respectively adding a protective solution into the sodium salt solution to dilute the sodium salt solution to obtain disodium salt standard solutions with a plurality of sodium salt concentrations, wherein the sodium salt is bis [4- (N-ethyl-N-3-sulfobenzyl) aminophenyl ] -2-sulfomethylphenyl disodium salt, for example, the first sodium salt concentration is 5mg/L, the second sodium salt concentration is 50mg/L and the third sodium salt concentration is 1mg/L in the three disodium salt standard solutions;
respectively scribing reference scribing lines on the three substrate films by using disodium salt standard solution with first sodium salt concentration, scribing first scribing lines on the first substrate film by using disodium salt standard solution with first sodium salt concentration, scribing second scribing lines on the second substrate film by using disodium salt standard solution with second sodium salt concentration, and scribing third scribing lines on the third substrate film by using disodium salt standard solution with third sodium salt concentration; the two scribe lines on each substrate film are parallel;
after drying, two marking lines on each substrate film respectively form a fluorescent strip to obtain the quality control card;
after drying, forming a reference fluorescent strip by the reference scribing lines on the substrate films, forming a first fluorescent strip by the first scribing lines on the first substrate film, forming a second fluorescent strip by the second scribing lines on the second substrate film, and forming a third fluorescent strip by the third scribing lines on the third substrate film; the reference fluorescence band on the first substrate film is parallel to the first fluorescence band, the reference fluorescence band on the second substrate film is parallel to the second fluorescence band, and the reference fluorescence band on the third substrate film is parallel to the third fluorescence band.
Example 5
Dissolving sodium salt to form sodium salt solution, and obtaining mother liquor with the sodium salt concentration of 1000 mg/L; dissolving trehalose, bovine serum albumin and glycerol in pure water, and mixing uniformly to form a protective solution; wherein, in the protective solution: the trehalose concentration was 5%, the bovine serum albumin concentration was 4%, and the glycerol concentration was 1%.
Respectively adding a protective solution into the sodium salt solution to dilute the sodium salt solution to obtain disodium salt standard solutions with a plurality of sodium salt concentrations, wherein the sodium salt is bis [4- (N-ethyl-N-3-sulfobenzyl) aminophenyl ] -2-sulfomethylphenyl disodium salt, for example, the first sodium salt concentration is 5mg/L, the second sodium salt concentration is 50mg/L and the third sodium salt concentration is 100mg/L in the three disodium salt standard solutions;
respectively scribing reference scribing lines on the three substrate films by using disodium salt standard solution with first sodium salt concentration, scribing first scribing lines on the first substrate film by using disodium salt standard solution with first sodium salt concentration, scribing second scribing lines on the second substrate film by using disodium salt standard solution with second sodium salt concentration, and scribing third scribing lines on the third substrate film by using disodium salt standard solution with third sodium salt concentration; the two scribe lines on each substrate film are parallel;
after drying, two marking lines on each substrate film respectively form a fluorescent strip to obtain the quality control card;
after drying, forming a reference fluorescent strip by the reference scribing lines on the substrate films, forming a first fluorescent strip by the first scribing lines on the first substrate film, forming a second fluorescent strip by the second scribing lines on the second substrate film, and forming a third fluorescent strip by the third scribing lines on the third substrate film; the reference fluorescence band on the first substrate film is parallel to the first fluorescence band, the reference fluorescence band on the second substrate film is parallel to the second fluorescence band, and the reference fluorescence band on the third substrate film is parallel to the third fluorescence band.
Unless defined otherwise, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention belongs. The use of "first," "second," and similar terms in the present application do not denote any order, quantity, or importance, but rather the terms are used to distinguish one element from another. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships are changed accordingly.
While the foregoing is directed to the preferred embodiment of the present invention, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (10)
1. A preparation method of a quality control card is characterized by comprising the following steps:
dissolving sodium salt to form sodium salt solution;
placing trehalose, bovine serum albumin and glycerol in a solvent to form a protective solution;
adding the protective solution to the sodium salt solution respectively to dilute the sodium salt solution to obtain a disodium salt standard solution with a first sodium salt concentration, a second sodium salt concentration and a third sodium salt concentration;
respectively scribing reference scribing lines on the three substrate films by using disodium salt standard solution with first sodium salt concentration, scribing first scribing lines on the first substrate film by using disodium salt standard solution with first sodium salt concentration, scribing second scribing lines on the second substrate film by using disodium salt standard solution with second sodium salt concentration, and scribing third scribing lines on the third substrate film by using disodium salt standard solution with third sodium salt concentration;
wherein the two scribe lines on each of the substrate films are parallel, the first sodium salt concentration is different from the second sodium salt concentration, and the first sodium salt concentration is different from the third sodium salt concentration; after drying, two marking lines on each substrate film respectively form a fluorescent strip to obtain the quality control card;
the sodium salt is bis [4- (N-ethyl-N-3-sulfonic acid benzyl) aminophenyl ] -2-sulfonic acid tolyl disodium salt.
2. The method of claim 1, wherein the forming the protective solution by placing trehalose, bovine serum albumin and glycerol in a solvent comprises:
dissolving trehalose, bovine serum albumin and glycerol in pure water, and uniformly mixing to form the protective solution.
3. The method according to claim 1, wherein the concentration of the sodium salt in the disodium salt standard solution is 1 to 100 mg/L.
4. The production method according to claim 1, characterized in that, in the protective solution:
the concentration of trehalose is 1-10%;
the concentration of bovine serum albumin is 1-10%;
the concentration of glycerol is 0.1-5%.
5. The production method according to claim 4, characterized in that, in the protective solution:
the concentration of trehalose is 5%;
the concentration of bovine serum albumin is 4%;
the concentration of glycerol was 1%.
6. A quality control card, comprising:
the fluorescent film comprises three substrate films, wherein each substrate film is respectively provided with a reference fluorescent strip, a first fluorescent strip is formed on the first substrate film, a second fluorescent strip is formed on the second substrate film, and a third fluorescent strip is formed on the third substrate film;
wherein the two fluorescence bands on each substrate film are parallel, and the reference fluorescence band, the first fluorescence band, the second fluorescence band and the third fluorescence band respectively have sodium salt, trehalose, bovine serum albumin and glycerol therein; the reference fluorescence band is the same as the first fluorescence band, the sodium salt content in the first fluorescence band is different from that in the second fluorescence band, and the sodium salt content in the first fluorescence band is different from that in the third fluorescence band;
the sodium salt is bis [4- (N-ethyl-N-3-sulfonic acid benzyl) aminophenyl ] -2-sulfonic acid tolyl disodium salt.
7. The quality control card according to claim 6, wherein the trehalose content in each of the fluorescent strips is 1 to 10 parts by weight;
the content of bovine serum albumin is 1-10 parts by weight;
the content of glycerin is 0.1-5 weight parts.
8. The quality control card according to claim 7, wherein the trehalose content in each of the fluorescent strips is 5 parts by weight;
the bovine serum albumin content was 4 parts by weight;
the content of glycerin was 1 part by weight.
9. The quality control card of claim 6, wherein the second fluorescent strip has a different sodium salt content than the third fluorescent strip.
10. The quality control card of claim 6, further comprising:
the bottom plate, be equipped with the recess on the bottom plate, the substrate membrane is established in the recess.
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