CN112680336A - Calibrating device of optical traceability standard device of fluorescent quantitative PCR instrument - Google Patents
Calibrating device of optical traceability standard device of fluorescent quantitative PCR instrument Download PDFInfo
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- CN112680336A CN112680336A CN202110271224.6A CN202110271224A CN112680336A CN 112680336 A CN112680336 A CN 112680336A CN 202110271224 A CN202110271224 A CN 202110271224A CN 112680336 A CN112680336 A CN 112680336A
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Abstract
The application discloses a calibrating device of an optical traceability standard device of a fluorescence quantitative PCR instrument, which comprises a measurement closed environment area and an automatic control device area; the measurement closed environment area adopts a light-shading design to form a closed dark environment, and a three-axis positioning system and an optical measurement unit are arranged in the measurement closed environment area; the automation control device area is provided with an automation control device. The device aims at the light source position and the height of fluorescence quantitative PCR appearance optics traceability standard device with optical sensor through automatic aversion and positioning control device's mode under airtight dark environment condition and simulated temperature environment condition, measures the optical parameter of its light source change in-process, realizes measuring spectral range: 380-780nm, resolution of 1.56 nm/pixel, spectral accuracy of +/-1 nm and brightness accuracy of +/-2%. And the CCD field monitoring device is used for carrying out photoelectric conversion on the collected field and displaying the converted field to the display module for positioning and visualization operation.
Description
Technical Field
The application relates to the field of optical device calibration, in particular to a calibration device of an optical traceability standard device of a fluorescence quantitative PCR instrument.
Background
The core technology of the fluorescent quantitative PCR instrument is a method for adding fluorescent groups into a PCR reaction system, monitoring the whole PCR reaction process in real time by using fluorescent signal accumulation, and finally carrying out quantitative analysis on an unknown template through a standard curve, wherein the method mainly relates to contents of thermal and optical parts, and the parameters of the optical part and the optical performance of the instrument are mainly calibrated and traced by two methods of a biological DNA standard substance, a chemical fluorescent dye and an optical physical method, which are collectively called as three methods of biology, chemistry and physics. The biological and chemical methods have full defects in the accuracy and process analysis, and the purpose is to provide a contrast method for the detection result of the instrument, and the process is not traceable; the scientificity, the rigor and the traceability of the method are obviously insufficient, and the method belongs to consumables, cannot be repeatedly used and has high long-term cost. The device for calibrating the fluorescence quantitative PCR instrument by adopting the optical physical method is called a fluorescence quantitative PCR instrument optical traceability standard device, and has the main functions of simulating a standard light source for providing fluorescence brightness change conditions in the PCR reaction process, simultaneously acquiring temperature indexes in a reaction system, acquiring the change conditions of the standard light source brightness by using the fluorescence quantitative PCR instrument, analyzing and comparing the change conditions, further judging the optical performance of the fluorescence quantitative PCR instrument, and judging the influence of the temperature conditions on the optical performance, thereby realizing the judgment and evaluation of the optical performance of the fluorescence quantitative PCR instrument. However, no method and corresponding equipment are available for carrying out superior traceability on an optical traceability standard device of a fluorescence quantitative PCR instrument for carrying out an optical physical method.
Disclosure of Invention
In order to calibrate the optical performance of the optical traceability standard device of the fluorescence quantitative PCR instrument, ensure that the test conditions meet the requirements of measuring the brightness and the spectral range of the light source output by the simulated fluorescent substance in the amplification reaction of the optical traceability standard device of the fluorescence quantitative PCR instrument under the actual application environment condition, realize the automation and the visualization operation of the measurement process and ensure that the measured result has the traceability to the upper standard traceability,
the invention provides a device for measuring accuracy and calibrating optical performance provided by an optical traceability standard device of a fluorescent quantitative PCR instrument, namely a calibrating device of the optical traceability standard device of the fluorescent quantitative PCR instrument.
The calibration device comprises a measurement closed environment area and an automatic control device area;
the measurement closed environment area adopts a light-shading design to form a closed dark environment, and a three-axis positioning system and an optical measurement unit are arranged in the measurement closed environment area; the automatic control device area is provided with an automatic control device;
the optical measurement unit comprises an optical sensor and a CCD (charge coupled device) view field monitoring device; the optical sensor measures the optical parameters of the optical traceability standard device of the fluorescence quantitative PCR instrument; the CCD view field monitoring device is used for view field positioning and observation of the optical sensor measuring position.
The three-axis positioning system comprises a transmission device, a distance measuring device and a bracket; the transmission device drives the optical measurement unit fixed on the bracket or the optical traceability standard device of the fluorescence quantitative PCR instrument to be measured to move in the X, Y and Z three-axis directions, so as to find and position the position of the light-emitting point of the optical traceability standard device of the fluorescence quantitative PCR instrument and scan according to the instruction of the automatic control device; the distance measuring device is used for measuring the movement stroke of the optical measuring unit or the optical traceability standard device of the fluorescent quantitative PCR instrument to be measured;
and the automatic control device is used for realizing automatic control on the movement of the three-axis positioning system.
Furthermore, a temperature condition simulation device is further arranged in the measurement closed environment area and provides temperature environment condition control for the optical traceability standard device of the fluorescence quantitative PCR instrument to be measured, so that the temperature environment condition is consistent with the environment condition applied by the device to be measured.
Further, the calibration apparatus further includes at least one of the following (1) to (6):
(1) the sealed environment area is measured by adopting a sealed cabin, a dark room or a dark box;
(2) the optical parameters comprise luminance, spectral power distribution, luminance linearity, color temperature and/or dominant wavelength;
(3) the optical sensor is a luminance meter, an imaging luminance meter, a spectrometer and/or a spectrophotometer;
(4) the automatic control device comprises a control module;
(5) the temperature condition simulation device is an automatic temperature control metal module;
(6) the optical sensor adopts a photographing type measuring mode, images all point positions in a measuring area at one time, or adopts a measuring mode of single-point measurement and automatic point-by-point scanning.
Further, the calibration apparatus further includes at least one of the following (1) to (6):
(1) the calibration device further comprises a human-computer interaction module, wherein the human-computer interaction module comprises a control panel and a display module;
(2) the calibration device also comprises an alarm module, and when the bracket drives the optical measurement unit or the optical traceability standard device of the fluorescence quantitative PCR instrument to be measured to move beyond a preset stroke, the alarm module sends an alarm signal;
(3) the measurement closed environment area also comprises an illuminating device, and the illuminating device is used for providing a light source for the CCD view field monitoring device during working;
(4) the measurement enclosed environment zone is provided with an opening to facilitate manual operation within the measurement enclosed environment zone;
(5) the automatic control device area is provided with a heat dissipation device for adjusting the temperature of the automatic control device area;
(6) the calibration device further comprises a power supply system.
Furthermore, the automatic control device can realize the control of the lighting device, the alarm module, the heat dissipation device and the temperature condition simulation device.
Further, the calibration device further includes at least one of the following (1) to (3):
(1) the optical measurement unit can be matched with different optical filters to measure in different spectral ranges;
(2) the calibration device adopts a cabinet body design, and a group of universal wheels are arranged at the bottom of the cabinet body so as to facilitate the movement of the device;
(3) and a shading hairbrush is arranged at an opening of the measurement closed environment area and used for blocking an external light source.
Furthermore, the calibration device further comprises a standard light source, wherein the standard light source is arranged in the measurement closed environment area, and can be compared with the device to be measured in the detection range of the optical measurement unit by setting the standard light source, so that the measurement error is corrected.
Further, the travel of the three-axis positioning system 3 in the X-axis direction is set to be 0-100mm, the travel in the Y-axis direction is set to be 0-100mm, and the travel in the Z-axis direction is set to be 0-350 mm.
Further, the variation range of the light-emitting brightness of the optical tracing standard device of the fluorescent quantitative PCR instrument to be detected is between 0% and 100%, or between 0 and 1000 cd/m.
Furthermore, when the optical measurement unit or the optical traceability standard device of the fluorescent quantitative PCR instrument to be measured is inaccurate in position, the three-axis positioning system can also be finely adjusted in a manual positioning mode.
The beneficial effects of the invention include: the calibration device of the optical traceability standard device of the fluorescence quantitative PCR instrument adopts the mode of automatically shifting and positioning the control device to align the optical sensor to the light source position and height of the optical traceability standard device of the fluorescence quantitative PCR instrument under the conditions of closed dark environment and simulated temperature environment, measures the optical parameters of the light source in the change process, such as the brightness, the peak wavelength, the spectral power distribution range and the like, and realizes the measurement in the spectral range: 380-780nm, resolution of 1.56 nm/pixel, spectral accuracy of +/-1 nm and brightness accuracy of +/-2%. And the CCD field monitoring device is used for carrying out photoelectric conversion on the collected field and displaying the converted field to the display module for positioning and visualization operation. The adopted optical sensor can carry out superior traceability calibration, and the accuracy and the effectiveness of the measurement result are ensured. The device is used as a superior standard device, the optical parameter accuracy is transmitted downwards to the optical traceability standard device of the fluorescent quantitative PCR instrument in the metering field, and then the internal optical parameters of the fluorescent quantitative PCR instrument for gene amplification are traced, and the device can be traced upwards to the national standard.
The calibrating device of the optical traceability standard device of the fluorescence quantitative PCR instrument solves the problem that the optical parameter calibrating device for the fluorescence quantitative PCR instrument by a physical method cannot perform traceability on an upper-level standard, fills the blank, measures the optical parameters of the fluorescence quantitative PCR by the physical method, can perform reverse traceability and process analysis on the accuracy of the measurement result, has more rigor and scientificity compared with a biological standard substance and chemical dye method, provides a more accurate implementation mode in a technical method, and provides a new mode in principle.
Drawings
FIG. 1 is a schematic structural diagram of a calibration device of an optical traceability standard device of a fluorescent quantitative PCR instrument according to the present invention;
FIG. 2 is a schematic diagram of the calibrating apparatus of the optical traceability standard apparatus of the fluorescence quantitative PCR apparatus of the present invention;
FIG. 3 is a flowchart illustrating the operation of the calibrating apparatus for the optical traceability standard apparatus of the fluorescence quantitative PCR apparatus according to the present invention;
FIG. 4 is a schematic structural diagram of a three-axis positioning system of the calibrating apparatus of the optical traceability standard apparatus of the fluorescent quantitative PCR apparatus of the present invention;
the system comprises a measurement device, a three-axis positioning system, an optical measurement unit, a temperature condition simulation device, a device to be measured, a control panel, a display module and universal wheels, wherein the measurement device comprises a measurement enclosed environment area 1, an automatic control device area 2, a three-axis positioning system 4, a temperature condition simulation device 5, a device to be measured 6, a control panel 7, a display module 8 and universal wheels 9.
Detailed Description
The following examples and their specific illustrations are intended to illustrate the invention and are not to be construed as limiting the invention.
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
The relative arrangement of the components and steps, the numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present invention unless specifically stated otherwise. Meanwhile, it should be understood that the sizes of the respective portions shown in the drawings are not drawn in an actual proportional relationship for the convenience of description. Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail, but are intended to be part of the specification where appropriate. In all examples shown and discussed herein, any particular value should be construed as merely illustrative, and not limiting. Thus, other examples of the exemplary embodiments may have different values. It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, further discussion thereof is not required in subsequent figures.
As shown in the attached figure 1, the calibration device in the embodiment adopts a cabinet body design, and a group of universal wheels 9 are arranged at the bottom of the cabinet body, so that the device can be conveniently moved. The cabinet body is divided into a measurement closed environment area 1 and an automatic control device area 2. A man-machine interaction module is arranged outside the cabinet body and comprises a control panel 7 and a display module 8.
The measurement closed environment area 1 adopts a light-shading design to form a closed dark environment, can adopt a sealed cabin (a closed cabinet) and can also adopt other dark environment conditions such as a darkroom or a cassette. And a three-axis positioning system 3, an optical measurement unit 4 and a temperature condition simulation device 5 are arranged in the measurement closed environment area 1.
The optical measurement unit 4 comprises an optical sensor and a CCD (charge coupled device) view field monitoring device; measuring optical parameters of an optical traceability standard device of the fluorescence quantitative PCR instrument by using an optical sensor, and transmitting the measured optical parameters to a display module 8 through a data transmission line by using the optical sensor for photoelectric conversion, wherein the optical parameters comprise luminance brightness, spectral power distribution, luminance linearity, color temperature and/or dominant wavelength; the optical sensor can select a brightness meter, an imaging brightness meter, a spectrometer and/or a spectrophotometer, and can select different sensors to match with different auxiliary devices to achieve the purpose of measurement. The CCD view field monitoring device is used for view field positioning and observation of the optical sensor measuring position, and photoelectrically converting the measuring view field signal and transmitting the signal to the display module 8 through a data line.
The optical measurement unit can be matched with different optical filters to measure in different spectral ranges.
The three-axis positioning system 3 comprises a transmission device, a distance measuring device and a support, wherein the transmission device drives the optical measuring unit 4 fixed on the support to move in the directions of three axes of X, Y and Z, so that the optical measuring unit 4 can move in a three-dimensional space, the position of a light emitting point of the optical tracing standard device of the fluorescence quantitative PCR instrument can be found and positioned, and point-by-point moving scanning can be carried out according to the instruction of the automatic control device and a fixed track; the distance measuring device is used for measuring the movement stroke of the optical measuring unit. The device of the invention can also comprise an alarm module, when the bracket drives the movement stroke of the optical measurement unit to exceed the preset stroke, the alarm module sends an alarm signal to the man-machine interaction module, and the man-machine interaction module commands the automatic control device to send an instruction to adjust the work of the transmission device. The three-axis positioning system 3 can also adopt a manual positioning mode to finely adjust the movement stroke of the optical measurement unit 4. As shown in fig. 4, in the present embodiment, the three-axis positioning system 3 has a stroke set to 0 to 100mm in the X-axis direction, a stroke set to 0 to 100mm in the Y-axis direction, and a stroke set to 0 to 350mm in the Z-axis direction.
The temperature condition simulation device 5 provides temperature environment condition control for the device to be tested 6, so that the temperature environment condition is consistent with the environment condition applied by the device to be tested; in this embodiment, the temperature condition simulation apparatus 5 is an automatic temperature control metal module, and the control mode thereof may be controlled by a human-computer interaction module, manual setting and APP setting.
The measurement closed environment area 1 further comprises a device to be measured 6, and the device to be measured 6 is placed in contact with the automatic temperature control metal module and is placed in the monitoring range of the optical measurement unit. The device to be measured 6 is a device for simulating a fluorescent substance to output a physical light source in an amplification reaction to measure the accuracy of the optical performance of the fluorescence quantitative PCR instrument, and in the embodiment, the device to be measured is an optical traceability standard device of the fluorescence quantitative PCR instrument.
The measurement closed environment area 1 further comprises an illuminating device, and the illuminating device is used for providing a light source for the CCD view field monitoring device during working.
The measurement enclosed environment area 1 may further be provided with an opening for facilitating manual operation in the measurement enclosed environment area, and the opening is provided with a shading brush 10 for blocking an external light source.
The automation control device area 2 is provided with an automation control device and a heat dissipation device; the heat dissipation device is used for adjusting the temperature of the automatic control device area; the automatic control device comprises a control module, the control module outputs control signals by converting software commands of the upper computer, and controls the control operations of the three-axis positioning system 3 such as movement, positioning, zeroing and the like; the automatic control device can also realize the control of the lighting device, the alarm module, the heat dissipation device and the temperature condition simulation device 5.
The calibration device further comprises a power supply system, and the power supply system supplies power to the calibration device.
When the temperature measuring device is used, the power supply is switched on, the temperature condition simulation device 5 is started, the device to be measured 6 is placed into the opening of the measurement closed environment area 1, is in contact with the temperature condition simulation device 5, and is placed in the monitoring range of the optical measuring unit 4. The triaxial positioning system 3 is zeroed, a certain luminance (the luminance variation range is between 10% -100%, or between 0-1000 cd/m) of the device under test 6 is set, and the automatic control device moves the optical measurement unit 4 to the measurement point in a positioning manner. And the lighting device is turned on, the CCD view field monitoring device transmits a measurement view field signal to the human-computer interaction module, whether the measurement position of the view field is accurate or not is observed, if the measurement position is accurate, the three-axis positioning system 3 measures the optical parameters of the optical traceability standard device of the fluorescence quantitative PCR instrument according to the instruction of the automatic control device and the coordinates according to the fixed track, and the optical sensor transmits data to the display module 8. If the position is not accurate, the three-axis positioning system 3 can be finely adjusted manually, and measurement is carried out after the adjustment is finished. After the point location measurement, the point location measurement is moved to the next point location measurement, so that the point location scanning measurement is realized, the point location measurement of the next brightness value can be carried out after all the point location scanning measurement is finished, and the next point location can be moved after the measurement of a plurality of brightness values on a certain point location is realized.
Embodiment 2, calibrating device of fluorescence quantitative PCR appearance optics standard device of tracing to source
The calibration device in the embodiment is divided into a measurement closed environment area 1 and an automatic control device area 2. A man-machine interaction module is arranged outside the cabinet body and comprises a control panel 7 and a display module 8.
The measurement closed environment area 1 adopts a light-shading design to form a closed dark environment, can adopt a sealed cabin (a closed cabinet) and can also adopt other dark environment conditions such as a darkroom or a cassette. And a three-axis positioning system 3 and an optical measurement unit 4 are arranged in the measurement closed environment area 1.
The optical measurement unit 4 comprises an optical sensor and a CCD (charge coupled device) view field monitoring device; measuring optical parameters of an optical traceability standard device of the fluorescence quantitative PCR instrument by using an optical sensor, and transmitting the measured optical parameters to a display module 8 through a data transmission line by using the optical sensor for photoelectric conversion, wherein the optical parameters comprise luminance brightness, spectral power distribution, luminance linearity, color temperature and/or dominant wavelength; the optical sensor can select a brightness meter, an imaging brightness meter, a spectrometer and/or a spectrophotometer, and can select different sensors to match with different auxiliary devices to achieve the purpose of measurement. The CCD view field monitoring device is used for view field positioning and observation of the optical sensor measuring position, and photoelectrically converting the measuring view field signal and transmitting the signal to the display module 8 through a data line. The optical measurement unit 4 is fixedly arranged in the measurement closed environment area 1.
The optical measurement unit can be matched with different optical filters to measure in different spectral ranges.
The three-axis positioning system 3 comprises a transmission device, a distance measuring device and a support, wherein the transmission device drives the device to be measured 6 fixed on the support to move in the directions of three axes of X, Y and Z, so that the device to be measured 6 can move in a three-dimensional space, and can perform point-by-point moving scanning according to a fixed track according to an instruction of the automatic control device; the distance measuring device is used for measuring the movement stroke of the device to be measured 6. The device can also comprise an alarm module, when the movement stroke of the device to be tested 6 exceeds the preset stroke, the alarm module sends an alarm signal to the human-computer interaction module, and the human-computer interaction module commands the automatic control device to send out an instruction to adjust the work of the transmission device. The triaxial positioning system 3 can also adopt a manual positioning mode to finely adjust the movement stroke of the device to be measured 6.
The device to be measured 6 is fixed on the bracket of the triaxial positioning system 3 and is arranged in the monitoring range of the optical measurement unit. The device to be measured 6 is a device for simulating a fluorescent substance to output a physical light source in an amplification reaction to measure the accuracy of the optical performance of the fluorescent quantitative PCR instrument.
The measurement closed environment area 1 further comprises an illuminating device, and the illuminating device is used for providing a light source for the CCD view field monitoring device during working.
The measurement enclosed environment area 1 may further be provided with an opening for facilitating manual operation in the measurement enclosed environment area, and the opening is provided with a shading brush 10 for blocking an external light source.
The automation control device area 2 is provided with an automation control device and a heat dissipation device; the heat dissipation device is used for adjusting the temperature of the automatic control device area; the automatic control device comprises a control module, the control module outputs control signals by converting software commands of the upper computer, and controls the control operations of the three-axis positioning system 3 such as movement, positioning, zeroing and the like; the automatic control device can also realize the control of the lighting device, the alarm module and the heat dissipation device.
When the device is used, a power supply is switched on, the device to be measured 6 is placed into the device from the opening of the measurement closed environment area 1, is fixed on the bracket of the three-axis positioning system 3 and is arranged in the monitoring range of the optical measurement unit 4. The triaxial positioning system 3 is zeroed, the light emitting brightness (within the range of 10% -100% or between 0-1000 cd/m) of the device under test 6 is set, and the automatic control device moves the device under test 6 to the measurement point in a positioning manner. And (3) turning on the lighting device, transmitting a measurement view field signal to the display module 8 by the CCD view field monitoring device, observing whether the measurement position of the view field is accurate, if so, measuring the optical parameters of the optical traceability standard device of the fluorescence quantitative PCR instrument by the optical sensor according to the fixed track and the coordinates by the triaxial positioning system 3 according to the instruction of the automatic control device, and transmitting data to the display module 8. If the position is not accurate, the three-axis positioning system 3 can be finely adjusted manually, and measurement is carried out after the adjustment is finished. After the point location measurement, the point location measurement is moved to the next point location measurement, so that the point location scanning measurement is realized, the point location measurement of the next brightness value can be carried out after all the point location scanning measurement is finished, and the next point location can be moved after the measurement of a plurality of brightness values on a certain point location is realized.
The calibration device in this embodiment is added with a standard light source on the basis of the calibration device in embodiment 1, and the standard light source is arranged in the measurement enclosed environment area 1 and in the detection range of the optical measurement unit 4. The standard light source is arranged to compare the value with the value of the device to be measured, and then the measurement error is corrected. The calibration device of the optical traceability standard device of the fluorescence quantitative PCR instrument can be self-calibrated by adding the standard light source, so that the measurement accuracy of the optical sensor is improved, and the measured device is more accurate.
In addition, in this embodiment, a photographing type measurement mode is performed by using an imaging optical sensor, and all points in the measurement area are imaged once, so that the measurement mode of single-point measurement and automatic point-by-point scanning in embodiment 1 is replaced.
In the description of the present invention, it is to be understood that the orientation or positional relationship indicated by the orientation words such as "front, rear, upper, lower, left, right", "lateral, vertical, horizontal" and "top, bottom", etc. are usually based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and in the case of not making a reverse description, these orientation words do not indicate and imply that the device or element being referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore, should not be considered as limiting the scope of the present invention; the terms "inner and outer" refer to the inner and outer relative to the profile of the respective component itself.
Spatially relative terms, such as "above … …," "above … …," "above … …," "above," and the like, may be used herein for ease of description to describe one device or feature's spatial relationship to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if a device in the figures is turned over, devices described as "above" or "on" other devices or configurations would then be oriented "below" or "under" the other devices or configurations. Thus, the exemplary term "above … …" can include both an orientation of "above … …" and "below … …". The device may be otherwise variously oriented (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.
It should be noted that the terms "first", "second", and the like are used to define the components, and are only used for convenience of distinguishing the corresponding components, and the terms have no special meanings unless otherwise stated, and therefore, the scope of the present invention should not be construed as being limited.
Claims (10)
1. A calibration device of an optical traceability standard device of a fluorescence quantitative PCR instrument is characterized by comprising a measurement closed environment area and an automatic control device area;
the measurement closed environment area adopts a light-shading design to form a closed dark environment, and a three-axis positioning system and an optical measurement unit are arranged in the measurement closed environment area; the automatic control device area is provided with an automatic control device;
the optical measurement unit comprises an optical sensor and a CCD (charge coupled device) view field monitoring device; the optical sensor measures the optical parameters of the optical traceability standard device of the fluorescence quantitative PCR instrument; the CCD view field monitoring device is used for view field positioning and observation of the measurement position of the optical sensor;
the three-axis positioning system comprises a transmission device, a distance measuring device and a bracket; the transmission device drives the optical measurement unit fixed on the bracket or the optical traceability standard device of the fluorescence quantitative PCR instrument to be measured to move in the X, Y and Z three-axis directions, so as to find and position the position of the light-emitting point of the optical traceability standard device of the fluorescence quantitative PCR instrument and scan according to the instruction of the automatic control device; the distance measuring device is used for measuring the movement stroke of the optical measuring unit or the optical traceability standard device of the fluorescent quantitative PCR instrument to be measured;
and the automatic control device is used for realizing automatic control on the movement of the three-axis positioning system.
2. The calibrating device for the optical traceability standard device of the fluorescence quantitative PCR instrument as claimed in claim 1, wherein a temperature condition simulator is further arranged in the sealed environment measuring area, and the temperature condition simulator provides temperature environment condition control for the optical traceability standard device of the fluorescence quantitative PCR instrument to be measured, so that the temperature environment condition is consistent with the environment condition applied by the device to be measured.
3. The calibrating device for the optical traceability standard device of the quantitative fluorescence PCR instrument as claimed in claim 2, further comprising at least one of the following (1) - (6):
(1) the sealed environment area is measured by adopting a sealed cabin, a dark room or a dark box;
(2) the optical parameters comprise luminance, spectral power distribution, luminance linearity, color temperature and/or dominant wavelength;
(3) the optical sensor is a luminance meter, an imaging luminance meter, a spectrometer and/or a spectrophotometer;
(4) the automatic control device comprises a control module;
(5) the temperature condition simulation device is an automatic temperature control metal module;
(6) the optical sensor adopts a photographing type measuring mode, images all point positions in a measuring area at one time, or adopts a measuring mode of single-point measurement and automatic point-by-point scanning.
4. The calibrating device for the optical traceability standard device of the quantitative fluorescence PCR instrument as claimed in claim 3, further comprising at least one of the following (1) - (6):
(1) the calibration device further comprises a human-computer interaction module, wherein the human-computer interaction module comprises a control panel and a display module;
(2) the calibration device also comprises an alarm module, and when the bracket drives the optical measurement unit or the optical traceability standard device of the fluorescence quantitative PCR instrument to be measured to move beyond a preset stroke, the alarm module sends an alarm signal;
(3) the measurement closed environment area also comprises an illuminating device, and the illuminating device is used for providing a light source for the CCD view field monitoring device during working;
(4) the measurement enclosed environment zone is provided with an opening to facilitate manual operation within the measurement enclosed environment zone;
(5) the automatic control device area is provided with a heat dissipation device for adjusting the temperature of the automatic control device area;
(6) the calibration device further comprises a power supply system.
5. The calibrating device for the optical traceability standard device of the fluorescence quantitative PCR instrument as claimed in claim 4, wherein the automatic control device can control the illumination device, the alarm module, the heat dissipation device and the temperature condition simulation device.
6. The calibrating device for the optical traceability standard device of the quantitative fluorescence PCR instrument as claimed in claim 4, further comprising at least one of the following (1) - (3):
(1) the optical measurement unit can be matched with different optical filters to measure in different spectral ranges;
(2) the calibration device adopts a cabinet body design, and a group of universal wheels are arranged at the bottom of the cabinet body so as to facilitate the movement of the device;
(3) and a shading hairbrush is arranged at an opening of the measurement closed environment area and used for blocking an external light source.
7. The calibrating device for the optical traceability standard device of the fluorescence quantitative PCR instrument as claimed in any one of claims 1 to 6, further comprising a standard light source, wherein the standard light source is disposed in the measurement enclosed environment region, and can perform numerical comparison with the device to be measured by disposing the standard light source within the detection range of the optical measurement unit, thereby correcting the measurement error.
8. The calibrating device for the optical traceability standard unit of the quantitative fluorescence PCR instrument as claimed in any one of claims 1 to 6, wherein the three-axis positioning system has a stroke set to 0-300mm in the X-axis direction, 0-300mm in the Y-axis direction and 0-350mm in the Z-axis direction.
9. The calibration device for the optical tracing standard device of the fluorescent quantitative PCR instrument as claimed in any one of claims 1 to 6, wherein the variation range of the light emission brightness of the optical tracing standard device of the fluorescent quantitative PCR instrument to be tested is between 0% and 100%, or between 0-1000 cd/m.
10. The calibrating device for the optical traceability standard device of the fluorescence quantitative PCR instrument as claimed in any one of claims 1 to 6, wherein when the position of the optical measurement unit or the optical traceability standard device of the fluorescence quantitative PCR instrument to be measured is inaccurate, the three-axis positioning system can be finely adjusted by manual positioning.
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Inventor after: Li Zheng Inventor after: Fu Zhi Yong Inventor after: Sun Junfeng Inventor after: Zhou Wen Inventor after: Zhang Jun Inventor after: Wan Quanshou Inventor after: Zhao Dandan Inventor before: Li Zheng Inventor before: Fu Zhi Yong Inventor before: Zhang Chen Inventor before: Sun Junfeng Inventor before: Zhou Wen Inventor before: Zhang Jun Inventor before: Wan Quanshou Inventor before: Zhao Dandan |