WO2022183567A1 - Automatic calibration mechanism for fluorescence immunoassay analyser and automatic calibration method thereof - Google Patents

Abstract

An automatic calibration mechanism for a fluorescence immunoassay analyser and an automatic calibration method thereof, the automatic calibration mechanism comprising: a base (11), an optical module (12) fixed above the base (11); an outer ring frame (13) rotatably disposed above the base (11); a rotation drive module having a transmission connection to the outer ring frame (13); and a data processor having a wireless connection to the optical module (12); the outer ring frame (13) is provided with a standard substance, and the rotation drive module periodically drives the outer ring frame to rotate along a direction M or a direction N in order to control the standard substance (14) to be positioned directly below the optical module (12). The present automatic calibration mechanism is safe, reliable, and stable, does not produce consumables, saves costs and does not require manual operation, has fully automatic calibration and a high degree of automation, and greatly increases working efficiency.

Description

Automatic calibration mechanism and automatic calibration method for fluorescence immunoassay analyzer technical field

The present invention relates to the field of in vitro diagnostic POCT. More specifically, the present invention relates to an automatic calibration mechanism for a fluorescence immunoassay analyzer and an automatic calibration method thereof.

Background technique

In vitro diagnostic POCT is a kind of detection technology with great potential. It has the advantages of rapidity, simplicity, high efficiency, low cost, short test cycle, and small amount of specimens. It has been widely used in clinical practice. As a new development direction, POCT has developed rapidly in recent years.

In the field of in vitro diagnostic POCT, it is well known to use fluorescence immunoassay analyzers with different structures to achieve accurate and rapid detection of samples. In the process of researching and realizing the accurate and rapid detection of samples, the inventor found that the fluorescence immunoassay analyzer in the prior art has at least the following problems:

Optical modules in existing instruments are composed of multiple precision components and selected lenses, which play a vital role in data detection. With the accumulation of the use cycle of the instrument itself, the encounter or change of the external environment may affect the optical module, resulting in inaccurate detection and analysis results.

In view of this, it is necessary to develop an automatic calibration mechanism for a fluorescence immunoassay analyzer and an automatic calibration method thereof to solve the above problems.

SUMMARY OF THE INVENTION

In view of the deficiencies in the prior art, the main purpose of the present invention is to provide an automatic calibration mechanism for a fluorescence immunoassay analyzer and an automatic calibration method thereof, which periodically control the reference material to be located in the optical system by rotating the drive module. Just below the module, the optical module is automatically calibrated, which is safe, reliable and stable, without consumables, saving costs and no manual operation, fully automatic calibration, high degree of automation, greatly improving work efficiency, and has a broad market application value.

To achieve these objects and other advantages according to the present invention, there is provided an automatic calibration mechanism for a fluorescence immunoassay analyzer comprising: a base,

an optical module, which is fixed on the top of the base;

an outer ring frame, which is rotatably arranged above the base;

a rotating drive module, which is in driving connection with the outer ring frame; and

a data processor wirelessly connected to the optical module;

Wherein, the outer ring frame is provided with a standard substance, and the rotation driving module periodically drives the outer ring frame to rotate along the direction M or the direction N, so as to control the standard substance to be located directly under the optical module.

Preferably, the optical module includes: a fixed frame with a hollow interior; and

a laser emitter, a dichroic mirror, a first lens, an optical filter, a second lens, a pinhole diaphragm and a detector arranged inside the fixed frame;

Wherein, the first lens, the dichroic mirror, the filter, the second lens, the pinhole diaphragm and the detector are arranged in order from bottom to top along the vertical direction, and the first lens, the dichroic mirror, the filter The light sheet, the second lens, the pinhole diaphragm and the detector are arranged coaxially in the vertical direction, the laser emitter and the dichroic mirror are located at the same height in the vertical direction, and the dichroic mirror is arranged obliquely, so The detector is wirelessly connected to the data processor.

Preferably, the outer ring frame includes a fluorescence focusing portion, and a positioning groove is formed at the top of the fluorescence focusing portion, the positioning groove is adapted to the standard substance, and the standard substance is placed in the positioning groove;

The fluorescent focusing part is also provided with a fluorescent focusing groove, the fluorescent focusing groove is located directly below the positioning groove, the cross section of the fluorescent focusing groove is arc-shaped, and the surface of the fluorescent focusing groove serves as a light source. face treatment.

In order to achieve these objects and other advantages according to the present invention, there is also provided an automatic calibration method for a fluorescence immunoassay analyzer as described in any one of the above, characterized in that it comprises the following steps:

S1. Select the standard material;

S2. Establish a database of reference materials and optical modules;

S3. Periodically automatically collect the data information of the optical module and the reference material, and upload it to the database for analysis and comparison with the data in the database;

S4. Use the parameter correction table to correct the setting parameters of the optical module;

S5, the correction effect verification.

Preferably, the standard substance in step S1 is selected as ruby.

Preferably, the specific steps of establishing the database of the reference material and the optical module in step S2 are:

a1, the rotating drive module drives the outer ring frame to rotate along the direction M, so that the standard material is located directly below the optical module;

a2. Set the parameter low value A, the parameter median value B and the parameter high value C of the laser transmitter, and the laser transmitter scans the standard material for 20 rounds with the parameter low value A, the parameter median value B and the parameter high value C respectively;

a3. The detector obtains the data information of the standard material for 20 rounds of scanning by the laser transmitter with the parameter low value A, the parameter median value B and the parameter high value C respectively, and then uploads the obtained data information to the data processor for processing, so as to store the data in the data The database is generated in the processor.

Preferably, in step S3, the data information of the optical module and the reference material is periodically and automatically collected, and the specific steps of uploading it to the database and analyzing and comparing the data in the database are as follows:

b1, the rotating drive module drives the outer ring frame to rotate along the direction M, so that the standard material is located directly below the optical module;

b2. Set the parameter low value A, the parameter median value B and the parameter high value C to the laser transmitter, and the laser transmitter scans the standard material with the parameter low value A, the parameter median value B and the parameter high value C respectively;

b3. The detector acquires the data information of the reference material with the low value A, the median value B and the high value C of the parameter respectively scanned by the laser transmitter, and then uploads the obtained data information to the data processor and the data information of the database for analysis and comparison ;

b4. If the data information collected by the parameter low value A, the parameter median value B and the parameter high value C is within the preset range of the data information of the database, the data processor will record the collected data information and end the verification at the same time; If the data information collected by the parameter low value A, the parameter medium value B, and the parameter high value C is not within the preset range of the data information in the database, enter the parameter correction.

Preferably, the specific steps of using the parameter correction table to correct the setting parameters of the optical module in step S4 are:

c1. If the data analysis and comparison exceeds the preset range, take the current reading range as the benchmark to obtain the corresponding parameter value from the parameter comparison table, and modify the current parameter value of the laser transmitter to the obtained parameter value;

c2. After the initial parameter correction is completed, the laser transmitter repeatedly scans the reference material with the modified parameter value for verification until the verification is completed.

Preferably, the specific steps of verifying the correction effect in step S5 are:

d1. Select 4 fluorescence immunoassay analyzers, marked as No. 1, No. 2, No. 3 and No. 4, No. 1 and No. 2 instruments are not automatically calibrated, and No. 3 and No. 4 instruments are automatically calibrated every 50 days;

d2. Set the laser emitters of the No. 1 Fluorescence Immunoassay Analyzer, No. 2 Fluorescence Immunoassay Analyzer, No. 3 Fluorescence Immunoassay Analyzer, and No. 4 Fluorescence Immunoassay Analyzer to the same parameter value, and collect each Fluorescence Immunoassay Analyzer every 10 days. Data information of the optical module of the instrument, and record;

d3. Compare and analyze the collected data information of the optical modules of each fluorescence immunoassay analyzer.

One of the above technical solutions has the following advantages or beneficial effects: the standard material is periodically controlled to be located directly under the optical module by rotating the driving module, and the optical module is automatically calibrated, which is safe, reliable and stable, no consumables are generated, and the cost is saved. At the same time of cost, no manual operation is required, and the calibration is completed automatically. The degree of automation is high, which greatly improves the work efficiency and has a broad market application value.

Other advantages, objects, and features of the present invention will appear in part from the description that follows, and in part will be appreciated by those skilled in the art from the study and practice of the invention.

Description of drawings

In order to illustrate the technical solutions of the embodiments of the present invention more clearly, the accompanying drawings of the embodiments will be briefly introduced below. Obviously, the drawings in the following description only relate to some embodiments of the present invention, rather than limit the present invention. in:

1 is a top view of an automatic calibration mechanism for a fluorescence immunoassay analyzer proposed according to an embodiment of the present invention;

2 is a partial exploded cross-sectional view of an automatic calibration mechanism for a fluorescence immunoassay analyzer proposed according to an embodiment of the present invention;

3 is a partial cross-sectional view of an automatic calibration mechanism for a fluorescence immunoassay analyzer proposed according to an embodiment of the present invention;

4 is a partial cross-sectional view of an automatic calibration mechanism for a fluorescence immunoassay analyzer proposed according to an embodiment of the present invention;

5 is a fluorescence spectrum diagram of ruby in an automatic calibration method for a fluorescence immunoassay analyzer proposed according to an embodiment of the present invention;

FIG. 6 is a step diagram of an automatic calibration method for a fluorescence immunoassay analyzer proposed according to an embodiment of the present invention.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

In the drawings, the shapes and dimensions may be exaggerated for clarity, and the same reference numerals will be used throughout the drawings to refer to the same or like parts.

Unless otherwise defined, technical or scientific terms used herein should have the ordinary meaning as understood by one of ordinary skill in the art to which this invention belongs. The terms "first", "second" and similar terms used in the description of the patent application and the claims of the present invention do not denote any order, quantity or importance, but are only used to distinguish different components. Likewise, words such as "a," "an," or "the" do not denote a limitation of quantity, but rather denote the presence of at least one. Words like "include" or "include" mean that the elements or items appearing before "including" or "including" cover the elements or items listed after "including" or "including" and their equivalents, and do not exclude other component or object. "Up", "Down", "Left", "Right", etc. are only used to represent the relative positional relationship, and when the absolute position of the described object changes, the relative positional relationship may also change accordingly.

In the following description, terms such as center, thickness, height, length, front, back, rear, left, right, top, bottom, upper, lower, etc. are defined relative to the configurations shown in the various figures. , in particular, "height" corresponds to the size from top to bottom, "width" corresponds to the size from left to right, "depth" corresponds to the size from front to back, they are relative concepts, so it is possible to These or other orientations should not be construed as limiting terms because they vary accordingly in different positions and different usage states.

Terms referring to attached, coupled, etc. (eg, "connected" and "attached") refer to the fixed or attached relationship, as well as the movable or rigidly attached relationship, of these structures, directly or indirectly, to each other through intervening structures, unless in the The other way is explicitly stated.

According to an embodiment of the present invention, combined with the illustrations in FIGS. 1 to 4 , it can be seen that the automatic calibration mechanism for the fluorescence immunoassay analyzer includes: a base 11 ,

The optical module 12 is fixed on the top of the base 11;

The outer ring frame 13 is rotatably arranged above the base 11;

a rotating drive module, which is connected with the outer ring frame 13 in a driving manner; and

a data processor, which is wirelessly connected to the optical module 12;

The outer ring frame 13 is provided with a standard substance 14, and the rotation driving module periodically drives the outer ring frame 13 to rotate along the direction M or the direction N, so as to control the standard substance 14 to be located in the optical module. directly below 12.

In a preferred embodiment of the present invention, the standard substance 13 is ruby.

It is understandable that ruby contains Cr element, which can undergo electronic transitions under the excitation of a specific wavelength of laser light.

With reference to FIG. 5 , it is a fluorescence spectrum of ruby under the irradiation of excitation light of different wavelengths.

The wave peaks in the figure are all around 691.3 nm, which varies with the wavelength of the excitation light, and can be used as a reference material 13 for verification.

The data processor is a tablet computer.

Further, the optical module 12 includes: a fixed frame body 121 with a hollow interior; and

a laser emitter 122, a dichroic mirror 123, a first lens 124, a filter 125, a second lens 126, a pinhole aperture 127 and a detector 128 disposed inside the fixed frame 121;

Wherein, the first lens 124, the dichroic mirror 123, the filter 125, the second lens 126, the pinhole diaphragm 127 and the detector 128 are arranged in order from bottom to top along the vertical direction, and the first lens 124, the dichroic mirror 123, the filter 125, the second lens 126, the pinhole diaphragm 127 and the detector 128 are arranged coaxially in the vertical direction, and the laser emitter 121 and the dichroic mirror 123 are arranged in the vertical direction. The directions are at the same height, the dichroic mirror 123 is tilted, and the detector 128 is wirelessly connected to the data processor.

Further, the outer ring frame 13 includes a fluorescence focusing portion 131, and a positioning groove 132 is formed at the top of the fluorescence focusing portion 131. The positioning groove 132 is adapted to the standard material 14, and the standard material 14 is placed in the positioning groove 132;

The fluorescence focusing part 131 is also provided with a fluorescence focusing groove 133. The fluorescence focusing groove 133 is located directly under the positioning groove 132. The cross section of the fluorescence focusing groove 133 is arc-shaped. The surface of the groove 133 is smooth-finished.

It is understandable that, with reference to Figure 4, after the ruby is excited to be fluorescent, its fluorescence is three-dimensional luminescence. The traditional capture method is only collected by a lens on one side of the ruby, and the collection effect is poor, and most of the fluorescence cannot be effectively collected;

3, the present invention is provided with a fluorescent focusing groove 133 just below the positioning groove 132 where the ruby is placed, and at the same time, the surface of the fluorescent focusing groove 133 is subjected to smooth surface treatment, so that the fluorescence emitted by the lower end face of the ruby is in contact with the The light surface of the fluorescence focusing groove 133 is refracted immediately, and the fluorescence generated on the upper end face of the ruby is focused on one point, which greatly reduces the dissipation of fluorescence in other directions, and amplifies the fluorescence signal by increasing the collection surface area.

In a preferred embodiment of the present invention, the automatic calibration mechanism for the fluorescence immunoassay analyzer further comprises: an alarm, which is wirelessly connected to the data processor.

It is understandable that, when the automatic calibration mechanism for the fluorescence immunoassay analyzer is automatically calibrated and identified for many times, the data processor controls the alarm to issue an alarm to remind the staff to perform manual calibration.

To sum up, the rotation driving module periodically drives the outer ring frame 13 to rotate along the direction M or the direction N, so as to control the standard substance 14 to be located directly under the optical module 12, and the laser emits The excitation light emitted by the device 122 is refracted by the dichroic mirror 123 and then focused on the ruby through the first lens 124. The ruby is excited to emit fluorescence, and the excited fluorescence is focused and collected by the fluorescence focusing groove 133 to the first lens 124. The focal point of a lens 124 passes through the first lens 124 in parallel with the dichroic mirror 123 , the filter 124 , the second lens 126 and the pinhole diaphragm 127 and then reaches the detector 128, the detector 128 reads the fluorescence data and uploads the data to the data processor.

The excitation light is emitted by the laser on the right, refracted by the dichroic mirror and then focused on the 1# ruby by the lens, and the ruby is excited to fluoresce; the excited fluorescence is focused by the fluorescence focusing device and collected to the focus of the lens, and then parallel to the dichroic after the lens. After the mirror, filter, lens and pinhole aperture, it reaches the detector to read the signal.

With reference to Fig. 6, the present invention also provides an automatic calibration method for a fluorescence immunoassay analyzer as described above, comprising the following steps:

S1. Select the standard material;

S2. Establish a database of reference materials and optical modules;

S3. Periodically automatically collect the data information of the optical module and the reference material, and upload it to the database for analysis and comparison with the data in the database;

S4. Use the parameter correction table to correct the setting parameters of the optical module;

S5, the correction effect verification.

Further, the standard substance in step S1 is selected as ruby.

It is understandable that ruby contains Cr element, which can undergo electronic transitions under the excitation of a specific wavelength of laser light.

With reference to FIG. 5 , it is a fluorescence spectrum of ruby under the irradiation of excitation light of different wavelengths.

The wave peaks in the figure are all around 691.3 nm, which varies with the wavelength of the excitation light, and can be used as a reference material 13 for verification.

Further, the specific steps of establishing the database of the reference material and the optical module in step S2 are:

a1, the rotating drive module drives the outer ring frame 13 to rotate along the direction M, so that the standard material 14 is located directly below the optical module 12;

a2. The laser transmitter 122 is set to the parameter low value A, the parameter median value B and the parameter high value C, and the laser transmitter 122 scans the standard material for 1420 rounds with the parameter low value A, the parameter median value B and the parameter high value C respectively;

a3. The detector 128 acquires the data information of the laser transmitter 122 scanning the standard material 14 with the low parameter value A, the median value B and the high parameter C respectively for 20 rounds, and then uploads the obtained data information to the data processor for processing, to generate the database in the data processor.

In a preferred mode of the present invention, the parameter adjustable range of the laser transmitter 122 is 0-110.

In an embodiment of the present invention, set the parameter low value A=1, the parameter median value B=10, and the parameter high value C=100, take the ruby and place it in the positioning groove 132, respectively use the parameter low value A=1, the parameter The median value B=10, the parameter high value C=100, the ruby was scanned for 20 rounds, and the obtained data information is shown in the following table:

serial number	low value A	median B	High value C		Remark
1	1.01	10.02	100.02
2	1.01	10.00	100.02
3	1.01	10.01	100.03

4	1.01	10.00	100.00
5	1.00	10.02	100.03
6	1.01	10.01	100.00
7	1.00	10.01	100.03
8	1.00	10.02	100.00
9	1.01	10.01	100.01
10	1.00	10.01	100.01
11	1.00	10.02	100.02
12	1.00	10.00	100.02
13	1.00	10.00	100.03
14	1.00	10.00	100.01
15	1.00	10.02	100.05
16	1.00	10.01	100.00
17	1.00	10.02	100.04
18	1.00	10.02	100.05
19	1.00	9.99	100.00
20	1.01	10.00	100.05
CV	0.49%	0.09%	0.02%

Combining the data in the above table, it can be seen that the ruby has been excited by multiple rounds of excitation light and emits stable and reliable fluorescence, which can be used as an effective basis for comparison. The specific values can be used as database data for data analysis and comparison.

Further, in step S3, the data information of the optical module and the standard substance is periodically and automatically collected, and the specific steps of uploading it to the database and analyzing and comparing the data in the database are as follows:

b1, the rotating drive module drives the outer ring frame 13 to rotate along the direction M, so that the standard material 14 is located directly below the optical module 12;

b2. Set the parameter low value A, the parameter median value B and the parameter high value C to the laser transmitter 122, and the laser transmitter 122 scans the standard material 14 with the parameter low value A, the parameter median value B and the parameter high value C respectively;

b3. The detector 128 acquires the data information of the standard material 14 by the laser transmitter 122 respectively using the parameter low value A, the parameter median value B and the parameter high value C to scan the data information of the standard material 14, and then uploads the obtained data information to the data processor and the data information of the database for processing. analysis and comparison;

b4. If the data information collected by the parameter low value A, the parameter median value B and the parameter high value C is within the preset range of the data information of the database, the data processor will record the collected data information and end the verification at the same time; If the data information collected by the parameter low value A, the parameter medium value B, and the parameter high value C is not within the preset range of the data information in the database, enter the parameter correction.

In an embodiment of the present invention, the laser transmitter 122 in the optical module 12 is set to three groups of parameters when it is factory-set, which are the parameter low value A, the parameter median value B, and the parameter high value C, respectively. The acceptance range is respectively A±10%, B±10%, C±10% as qualified, the rest are unqualified.

Further, in step S4, the specific steps of using the parameter correction table to correct the setting parameters of the optical module are:

c1, if the data analysis and comparison exceeds the preset range, take the current reading range as a benchmark to obtain the corresponding parameter value in the parameter comparison table, and modify the current parameter value of the laser transmitter 122 to the obtained parameter value;

c2. After the initial parameter correction is completed, the laser transmitter 122 repeatedly scans the reference material 14 with the modified parameter values for verification until the verification ends.

In a preferred embodiment of the present invention, if the parameter correction cannot be completed after three times of correction, the data processor controls the alarm device to issue an alarm to remind the staff to perform manual calibration.

In an embodiment of the present invention, the following parameter correction table can be used to read data corresponding to correction coefficient correction parameters.

serial number	read value		Correction coefficient
1	5.9	+0.2
2	5.8	+0.1
3	5.7	0
4	5.6	-0.1
5	5.5	-0.2

Further, the specific steps of the correction effect verification in step S5 are:

d1. Select 4 fluorescence immunoassay analyzers, marked as No. 1, No. 2, No. 3 and No. 4, No. 1 and No. 2 instruments are not automatically calibrated, and No. 3 and No. 4 instruments are automatically calibrated every 50 days;

d2. Set the laser emitters 122 of the No. 1 fluorescent immunoassay analyzer, No. 2 fluorescent immunoassay analyzer, No. 3 fluorescent immunoassay analyzer, and No. 4 fluorescent immunoassay analyzer to the same parameter value, and collect each fluorescent immunoassay every 10 days. Data information of the optical module 12 of the analyzer, and record;

d3. Compare and analyze the collected data information of the optical module 12 of each fluorescence immunoassay analyzer.

In an embodiment of the present invention, the parameter value of the laser emitter 122 of the No. 1 fluorescence immunoassay analyzer, No. 2 fluorescent immunoassay analyzer, No. 3 fluorescent immunoassay analyzer, and No. The data information of the optical module 12 of each fluorescence immunoassay analyzer was collected in 10 days, and recorded in the following table;

days		Unit 1	Unit 2	Unit 3	Unit 4	Remark
10	10.02	10.11	10.06	10.03
20	10.12	10.22	10.05	10.09
30	10.13	10.22	10.16	10.14
40	10.24	10.33	10.18	10.25
50	10.35	10.35	10.26	10.38	automatic verification
60	10.45	10.36	10.03	10.02
70	10.46	10.46	10.09	10.12
80	10.45	10.42	10.12	10.17
90	10.52	10.49	10.19	10.24
100	10.55	10.52	10.22	10.29	automatic verification

In the above table, the data values detected by the No. 1 and No. 2 fluorescent immunoassay analyzers increase by the number of days. With the accumulation of the instrument's own life cycle, the encounter or change of the external environment affects the optical module, resulting in the gradual change of the detected data. However, the No. 3 fluorescence immunoassay analyzer and the No. 4 fluorescence immunoassay analyzer can return to normal after automatic calibration. The automatic calibration method is effective, safe, reliable and stable.

To sum up, the present invention provides an automatic calibration mechanism for a fluorescence immunoassay analyzer and an automatic calibration method thereof, which periodically control the reference material to be located directly under the optical module by rotating the driving module, so that the optical module can be adjusted accordingly. Automatic calibration, safe, reliable and stable, no consumables, cost saving and no manual operation, fully automatic calibration, high degree of automation, greatly improved work efficiency, and has broad market application value.

The number of apparatuses and processing scales described here are intended to simplify the description of the present invention. Applications, modifications and variations to the present invention will be apparent to those skilled in the art.

Although embodiments of the present invention have been disclosed above, they are not limited to the applications set forth in the specification and embodiments. It can be fully adapted to various fields suitable for the present invention. Additional modifications can readily be implemented by those skilled in the art. Therefore, the invention is not to be limited to the specific details and illustrations herein shown and described, without departing from the general concept defined by the appended claims and the scope of equivalents.

Claims (9)

1. An automatic calibration mechanism for a fluorescence immunoassay analyzer, characterized in that it comprises: a base (11),

   an optical module (12), which is fixed above the base (11);

   an outer ring frame (13), which is rotatably arranged above the base (11);

   a rotary drive module, which is in driving connection with the outer ring frame (13); and

   a data processor, which is wirelessly connected to the optical module (12);

   Wherein, the outer ring frame (13) is provided with a standard substance (14), and the rotation driving module periodically drives the outer ring frame (13) to rotate along the direction M or the direction N, so as to control the standard substance (14). 14) is located directly below the optical module (12).
2. The automatic calibration mechanism for a fluorescence immunoassay analyzer according to claim 1, wherein the optical module (12) comprises: a fixed frame body (121) with a hollow interior; and

   a laser emitter (122), a dichroic mirror (123), a first lens (124), a filter (125), a second lens (126), and a pinhole light that are arranged inside the fixed frame (121) A stop (127) and a detector (128);

   Wherein, the first lens (124), the dichroic mirror (123), the filter (125), the second lens (126), the pinhole aperture (127) and the detector (128) extend from the vertical direction are arranged in sequence from bottom to top, and the first lens (124), the dichroic mirror (123), the filter (125), the second lens (126), the pinhole aperture (127) and the detector (128) are arranged coaxially in the vertical direction, the laser emitter (121) and the dichroic mirror (123) are located at the same height in the vertical direction, the dichroic mirror (123) is arranged obliquely, and the detector (128) ) is wirelessly connected to the data processor.
3. The automatic calibration mechanism for a fluorescence immunoassay analyzer according to claim 1, wherein the outer ring frame (13) comprises a fluorescence focusing part (131), and a top end of the fluorescence focusing part (131) is provided with a a positioning groove (132), the positioning groove (132) is adapted to the standard substance (14), and the standard substance (14) is placed in the positioning groove (132);

   The fluorescence focusing part (131) is further provided with a fluorescence focusing groove (133), the fluorescence focusing groove (133) is located directly below the positioning groove (132), and the fluorescence focusing groove (133) is horizontally arranged. The cross section is arc-shaped, and the surface of the fluorescent focusing groove (133) is treated with a smooth surface.
4. An automatic calibration method for a fluorescence immunoassay analyzer according to any one of claims 1 to 3, characterized in that it comprises the following steps:

   S1. Select the standard material;

   S2. Establish a database of reference materials and optical modules;

   S3. Periodically automatically collect the data information of the optical module and the reference material, and upload it to the database for analysis and comparison with the data in the database;

   S4. Use the parameter correction table to correct the setting parameters of the optical module;

   S5, the correction effect verification.
5. The automatic calibration method for a fluorescence immunoassay analyzer according to claim 4, wherein the standard substance in step S1 is selected as ruby.
6. The automatic calibration method of the fluorescence immunoassay analyzer according to claim 4, wherein the specific steps of establishing the database of the reference material and the optical module in step S2 are:

   a1, the rotating drive module drives the outer ring frame (13) to rotate along the direction M, so that the standard substance (14) is located directly below the optical module (12);

   a2. Set the parameter low value A, the parameter median value B and the parameter high value C to the laser transmitter (122), and the laser transmitter (122) scans the standard with the parameter low value A, the parameter median value B and the parameter high value C respectively Substance (14) 20 rounds;

   a3. The detector (128) acquires the data information of the laser transmitter (122) scanning the standard material (14) with the low parameter value A, the median value B and the high parameter C respectively for 20 rounds, and then uploads the obtained data information to the The data processor performs processing to generate a database in the data processor.
7. The automatic calibration method for a fluorescence immunoassay analyzer according to claim 4, characterized in that in step S3, the data information of the optical module and the standard substance is automatically collected periodically, and uploaded to the database and the data in the database is analyzed and compared The correct steps are:

   b1, the rotating drive module drives the outer ring frame (13) to rotate along the direction M, so that the standard substance (14) is located directly below the optical module (12);

   b2. The laser transmitter (122) is set to the low parameter value A, the median value B and the high value C of the parameter, and the laser transmitter (122) scans the standard with the low value A of the parameter, the median value B and the high value C of the parameter respectively. Substance (14);

   b3. The detector (128) acquires the data information of the standard material (14) by scanning the laser transmitter (122) with the parameter low value A, the parameter median value B and the parameter high value C respectively, and uploads the obtained data information to the data processor Analysis and comparison with the data information in the database;

   b4. If the data information collected by the parameter low value A, the parameter median value B and the parameter high value C is within the preset range of the data information of the database, the data processor will record the collected data information and end the verification at the same time; If the data information collected by the parameter low value A, the parameter medium value B, and the parameter high value C is not within the preset range of the data information in the database, enter the parameter correction.
8. The automatic calibration method for a fluorescence immunoassay analyzer according to claim 4, wherein the specific steps of using the parameter correction table to correct the set parameters of the optical module in step S4 are:

   c1. If the data analysis and comparison exceeds the preset range, take the current reading range as the benchmark to obtain the corresponding parameter value from the parameter comparison table, and modify the current parameter value of the laser transmitter (122) to the obtained parameter value ;

   c2. After the initial parameter correction is completed, the laser transmitter (122) repeatedly scans the reference material (14) with the modified parameter values for verification until the verification is completed.
9. The automatic calibration method for a fluorescence immunoassay analyzer according to claim 4, wherein the specific steps of verifying the correction effect in step S5 are:

   d1. Select 4 fluorescence immunoassay analyzers, marked as No. 1, No. 2, No. 3 and No. 4, No. 1 and No. 2 instruments are not automatically calibrated, and No. 3 and No. 4 instruments are automatically calibrated every 50 days;

   d2. Set the laser emitters (122) of the No. 1 Fluorescence Immunoassay Analyzer, No. 2 Fluorescence Immunoassay Analyzer, No. 3 Fluorescence Immunoassay Analyzer, and No. 4 Fluorescence Immunoassay Analyzer to the same parameter value, and collect each sample every 10 days. Data information of the optical module (12) of the fluorescence immunoassay analyzer, and record;

   d3. Compare and analyze the collected data information of the optical module (12) of each fluorescence immunoassay analyzer.

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